Rh5AG360200

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0014702
Physical Location & Seq
Forward (+)
60613095 .. 60619199
6105 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG360200.1

Sequence Viewer

Length: 3567 bp
ATGAAGAAGCGGAGGTATTGTGACTTTGCGGGTCGGTCTTCGACTAACGACGGTGAGACGACGGAAGGAGACCGACGTTCGTACTCGGGTCGGGTTTCGGGTTCGGACCAAACCGAAAACCTGGCCCGGAGAGAGTACTACGAAGACGGCGGTACGGATAGGCAACCGTTGAAACCGTCGCAGACGCATTTGAGCGGCGGCTTGCGTCAATGCGAATCACTGTCGATGAAGTTTCAGTGGAACAATCTGCTGGCCCAGAATCCGAATCCGGGTTCCAACCCGAGAAAGGTTCCGGGTCTGAACCGACCCGACTTGGATTATAGCAACCGGAGTGGTAGTTATAGACTGGATTATGAAATGGGTTATGGCGGAAGGGGTTTGGGTGTGGACCAGAGAAAGCAGTATGGGCATTTTGATCATCGTGGGTTCACGTCGCCGTTGCCGAAATCCCATCTTGGTCTCCAAGAAAACGAGGAGGACCATCGCCCAATCCCATATGTGACTGTGGAAGACGAGCCCAACTTTGCTTCTGGAACTGGGTATTCATCAATGGAGGGTTTTCGCGGCGTTCGAGATGAATACGACAGAAGCAGAGCAATGGCTGAGTTGCCAATAGGTCACCCGCCTAGACAGAATGAGTTGGTGGACTATGGAGGTTCATCAATTGCTTTGAGTGAGGGTTTTCGCGGGGAGTTCATTAGAAGCAGAGAAATGGGCCGTGGTGGATATTCTCAAGGTCAAACAGCGAGACACGATGAGTTGGTAGACAATGCAGGTGGTGCATCAGAGGCTTTGAATGAGGGTTTTCGCGGCAGAAGCAGAGCAATGGCTGAGGGGCCTGTTGGCAGCCATGCTGGATATTCTCAAGGACTTACGCCAAGACACTCACATGGTGAGCTCCCATTTGCTTTGAATGAGGGTTTTCGCGGTTCGGATGAGTTCAACAAAAGCAGAGCAATGGCTGAGGGGCCTATTGGCCATGGTGGATATTCTCATGGTCAAACACCAAGAAAGAATGAGTTGGTGCACCCCAGGTGGGCTCAGCAGAGTCCGAAAGAGGAAATGGGTAGGGACTACAAGGTTCCTGGAATGAAAAGAAATGACAATGGTGACATGGATGCCTTGTTCCTTCTTGACTTGCTCTATAAAAAGATGGCTACTGTTGGAGAAGACTCAAGGCCTCCAAGTCATTTGACGCCAAGCGTTGTTGATTCCGTTGTGTACAGGGTTGGTGGTAGCAATGGCTCTGTTGGAGCAGGCACTCATGAGAATTGCCAGTTGGACCAATATCATGATTATATGGAACCAACAAATGTTTTCCCCAACCATCAATCTACAAGTGAACCATCAATCCCCTGCTATGATGAAGTTTTGGGTTCTTCTAATTCAAGAACAGAGGTGTGCCGGAACAATGAAACCCAGGGCTTCAGATTAGGAGTAGATGTTGGTCATGAAAGAGAGGTCGAGCCTTTATCATATGAAGGTAACCTAGAGAGACTGGCTCTGCCTGATGAAGATCAAGGCTTGCTCGAGGCTTATGGTGGTTGCCAACGGAGGTTGGCAGCAGCCGAAAGCCTGGCATCCATGACCCCTCTGGGAAGGTGGGCATTGAGAAAAGTTGCCATGAAGGAGCAACAATCCGGCAATCACTCAAAGTCTAAGTTCTTCCCTGATTTGAACCCCTCTTGTAATGTACAGAACTCGAGCTATGAAGTTGACATGCAGTTGACTACTACTGAGGATATGCAGCAGCAATATGCTAACGAAGATATTCAACAATATGCTGCTACCTGTGGAGAGGATCACTGGAACATGGAGCAAAATGTGATCTGTGGAGATGGTCAATGTTCCATTGACCAAGGTGTGACCCGTGCAGTGGATCAAAGGAACACTGAACAAGGTTCCATTGACCAAGGTGTGACCTGTGGAGTGGATCAAAGGAACATTGCACAAGTTGTGACCTCTGGAGAGGATCAATGTAACATCCATCGAGGTGTGATCTCCAGAGGGGATCAATGGAACAATGATCAAGGTGTGACAACCTATGGAGAGCAACAATGGCGTATGGACAATTTAGGGGAACAATGGAACAATGATCAAGGCGTCACAACCTCTGGAGAGCAACAGTGGCGTATGAACTATGGAGAGGACCAATGGAATGCTGAGCATATCAACAACCTGAATTCTGACAGTTTGTTACCGAATTCTGAACTGCCAGCATCTACGCAAGAGCATTATGGCGAACAATCCAAATCTGCTAGGATACATGTAAAGGAGCGTTTGGGACCTCCCAAATTATCAACTGGAAGCATAAAAACGCGTTTGAGACCACCTATGAAATCACCTGGCAGTGTGAAGATGCGTTTGGGGCCTTCTGCAAATGATCCTAATATCAACATTTTAGATCCTAATATCAACATTTTAGGTTTCAAGCCTCGAGAACAATACAAATCTCGAATAAGAAAAACAGATGACTTCAGTGGAAGTATTCATCCTGAAGGGGATGGAATTCCAAAAGCTAAGACTGATGCTGCCGCTGGGAGCATTCATCCTCAGGCGAGTGCTCTTCCACAAGTCAAGACGGATGGTTTTATTGGAAGCATTCATCCTCAAGAGGGTGCTCATTCAATAGTTAAGACAGATGCTACTGGAAGCATTCATCCTCAAGGGAATGGTATTCCAAATGTTAAGAAAGGTGATTTTAATGGAAGCATTCGTCCTCAAGGGGGTGGTCTTTCAAAAGTTGATGTGAGAGCTGCAAAGAAAGAACCGCCTGAAGACTCTAAGGAGTTTAAGCAGCTGGTAGATGATGTCTTCATTAAGTTCATTAACATTTTGATTGAGAACCCACCTCAAAGAAGGAAAATCATGGAAGGAAAAGGAGGAAAGTGTTGCGTGTGTGCCAGCAATTCCAAAGAGTTTGTAGAAACGGCAACCTTGGCAAGGCATGCCTTCTTGTCGCCAAAGGTTGGTTTCAAAGCAGAACACCTGGGCTTTCATAAAGCACTTTGTGTGCTCTTGGGATGGGACAGTACAGCAGTCTCAAACACTAGGTGGGTTCAGCGGAACTTGCCAGATGCAGAATCCTTGGCTCTGAAGGAGGACCTAGTTATCTGGCCTCCTGTTGTTGTCATACACAATAGTTCTGTAGCAGATAAAAATCCTGAAGATGAAAGGCTGACGGTAAATGTTGAAGTTCTAGAGGCTATTCTTACAAGTATAGGATTTGTTGGGAGGAAGACCAATATGTACTGTGGGAAACCTGCCGATGGGAGTGTCATGGTGGTGAGGTTTCAGCCTACATTTTCTGGTTTGCAAGAAGCAGAAAGGCTTCACAAGCTGTTTGCTGATAACAAACATGGGAAGGCGGAGTGGCGGTATATCAATTCTGTTGGCCTCAGCAACAGCAGCAATGGAAAACAAGACTTAGAGGCTGATGAGGCGAATTGTCTATATGGTTATTTGGGAAATGCAGACGATTGGGATGAACTTGACTTGGGAACCAAGAAGCACTTCGGGGTGAAGAGCAAGAAACAAATCAAGGCTATTGCAAACGCTTCTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

1188

Amino Acids

131.23

Weight (kDa)

5.88

Isoelectric Point (pI)

39.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 1034 - 1162 7.2e-19 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014702)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g29670
malus_domestica MD03G1136700.v1.1
prunus_persica Prupe.6G121100_v2.0.a1 Prupe.6G121100_v2.0.a1
pyrus_communis pycom03g09250
rosa_chinensis RchiOBHm_Chr5g0055031
rosa_laevigata RLG00000034958
rosa_multiflora Rmu_co8511567.1_g000001 Rmu_sc0000925.1_g000013
rosa_roxburghii Rroxscaffold_1G00025460
rosa_rugosa Rorug05G0290200 Rorug05G0290300
rosa_samantha Rh5AG360200 Rh5BG371200 Rh5CG393500 Rh5DG385000
rosa_wichuraiana Rw5G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 764
Acc36I ACCTGC 2 cut(s) 764, 3271
AccB7I CCANNNNNTGG 1 cut(s) 2969
AccBSI CCGCTC 1 cut(s) 195
AccI GTMKAC 1 cut(s) 765
AccII CGCG 5 cut(s) 564, 687, 810, 927, 2320
AclWI GGATC 7 cut(s) 1809, 1887, 1941, 1980, 2019, 2378, 2399
AcoI YGGCCR 1 cut(s) 976
AcsI RAATTY 3 cut(s) 2182, 2203, 2508
AcuI CTGAAG 6 cut(s) 1411, 2461, 2517, 2796, 3116, 3186
AcyI GRCGYC 2 cut(s) 1196, 2103
AdeI CACNNNGTG 3 cut(s) 893, 3011, 3054
AfaI GTAC 7 cut(s) 83, 137, 154, 1223, 1695, 3034, 3251
AfiI CCNNNNNNNGG 9 cut(s) 269, 286, 1036, 1876, 2615, 2726, 2943, 2969, 3269
AflIII ACRYGT 2 cut(s) 2266, 2318
AjiI CACGTC 1 cut(s) 432
AjnI CCWGG 7 cut(s) 120, 1031, 1084, 1419, 1575, 2344, 2988
AjuI GAANNNNNNNTTGG 6 cut(s) 1262, 1294, 2348, 2380, 3497, 3529
AloI GAACNNNNNNTCC 4 cut(s) 1109, 1141, 1335, 1367
AluBI AGCT 6 cut(s) 898, 1707, 2519, 2756, 2800, 3340
AluI AGCT 6 cut(s) 898, 1707, 2519, 2756, 2800, 3340
Alw21I GWGCWC 5 cut(s) 900, 1029, 2566, 2623, 3018
Alw26I GTCTC 7 cut(s) 50, 63, 464, 742, 1489, 2320, 3046
Alw44I GTGCAC 1 cut(s) 1025
AlwI GGATC 7 cut(s) 1809, 1887, 1941, 1980, 2019, 2378, 2399
Ama87I CYCGRG 5 cut(s) 85, 280, 1529, 1702, 2436
ApaLI GTGCAC 1 cut(s) 1025
ApoI RAATTY 3 cut(s) 2182, 2203, 2508
Asp700I GAANNNNTTC 3 cut(s) 1770, 3330, 3512
AsuC2I CCSGG 3 cut(s) 127, 270, 294
AsuHPI GGTGA 8 cut(s) 65, 611, 905, 1121, 2334, 2708, 3298, 3532
AvaI CYCGRG 5 cut(s) 85, 280, 1529, 1702, 2436
AvaII GGWCC 7 cut(s) 106, 388, 478, 1282, 2149, 2285, 3103
AxyI CCTNAGG 1 cut(s) 2553
BaeGI GKGCMC 1 cut(s) 1029
BalI TGGCCA 1 cut(s) 978
BanII GRGCYC 3 cut(s) 519, 900, 1042
BbsI GAAGAC 7 cut(s) 30, 150, 516, 1176, 2784, 2806, 3245
Bbv12I GWGCWC 5 cut(s) 900, 1029, 2566, 2623, 3018
BbvCI CCTCAGC 3 cut(s) 831, 963, 3398
BceAI ACGGC 4 cut(s) 163, 421, 702, 2946
BciT130I CCWGG 7 cut(s) 122, 1033, 1086, 1421, 1577, 2346, 2990
BciVI GTATCC 1 cut(s) 2256
BclI TGATCA 3 cut(s) 415, 2026, 2095
BcnI CCSGG 3 cut(s) 127, 270, 294
BcoDI GTCTC 7 cut(s) 50, 63, 464, 742, 1489, 2320, 3046
BfaI CTAG 6 cut(s) 627, 1490, 2259, 3051, 3107, 3200
BfmI CTRYAG 1 cut(s) 3147
BfuAI ACCTGC 2 cut(s) 764, 3271
BfuI GTATCC 1 cut(s) 2256
BlpI GCTNAGC 2 cut(s) 1041, 2163
BmcAI AGTACT 1 cut(s) 137
Bme18I GGWCC 7 cut(s) 106, 388, 478, 1282, 2149, 2285, 3103
BmeT110I CYCGRG 5 cut(s) 85, 280, 1529, 1702, 2436
BmgBI CACGTC 1 cut(s) 432
BmrI ACTGGG 1 cut(s) 546
BmsI GCATC 8 cut(s) 791, 1108, 1589, 2228, 2349, 2518, 2632, 3067
BmuI ACTGGG 1 cut(s) 546
BpiI GAAGAC 7 cut(s) 30, 150, 516, 1176, 2784, 2806, 3245
BplI GAGNNNNNCTC 2 cut(s) 1486, 1518
BpmI CTGGAG 3 cut(s) 1986, 1987, 2136
Bpu10I CCTNAGC 3 cut(s) 831, 963, 3398
Bpu1102I GCTNAGC 2 cut(s) 1041, 2163
BpuEI CTTGAG 6 cut(s) 717, 849, 1159, 2595, 2649, 2706
BpuMI CCSGG 3 cut(s) 127, 270, 294
BsaBI GATNNNNATC 2 cut(s) 1515, 3159
BsaHI GRCGYC 2 cut(s) 1196, 2103
BsaI GGTCTC 3 cut(s) 63, 464, 2320
BsaWI WCCGGW 1 cut(s) 327
BsaXI ACNNNNNCTCC 7 cut(s) 34, 322, 352, 1428, 1458, 3362, 3392
Bsc4I CCNNNNNNNGG 9 cut(s) 269, 286, 1036, 1876, 2615, 2726, 2943, 2969, 3269
Bse1I ACTGG 7 cut(s) 351, 541, 1276, 1503, 1811, 2308, 2653
Bse21I CCTNAGG 1 cut(s) 2553
Bse3DI GCAATG 6 cut(s) 603, 831, 963, 1246, 1944, 3418
Bse8I GATNNNNATC 2 cut(s) 1515, 3159
BseBI CCWGG 7 cut(s) 122, 1033, 1086, 1421, 1577, 2346, 2990
BseJI GATNNNNATC 2 cut(s) 1515, 3159
BseLI CCNNNNNNNGG 9 cut(s) 269, 286, 1036, 1876, 2615, 2726, 2943, 2969, 3269
BseMI GCAATG 6 cut(s) 603, 831, 963, 1246, 1944, 3418
BseMII CTCAG 8 cut(s) 594, 822, 954, 1055, 1728, 2154, 2567, 3412
BseNI ACTGG 7 cut(s) 351, 541, 1276, 1503, 1811, 2308, 2653
BseRI GAGGAG 1 cut(s) 488
BseSI GKGCMC 1 cut(s) 1029
BseYI CCCAGC 1 cut(s) 2537
BsgI GTGCAG 1 cut(s) 1893
Bsh1236I CGCG 5 cut(s) 564, 687, 810, 927, 2320
BsiHKAI GWGCWC 5 cut(s) 900, 1029, 2566, 2623, 3018
BsiHKCI CYCGRG 5 cut(s) 85, 280, 1529, 1702, 2436
BsiSI CCGG 6 cut(s) 127, 269, 293, 328, 1405, 1641
BslFI GGGAC 3 cut(s) 1085, 2298, 3041
BslI CCNNNNNNNGG 9 cut(s) 269, 286, 1036, 1876, 2615, 2726, 2943, 2969, 3269
BsmAI GTCTC 7 cut(s) 50, 63, 464, 742, 1489, 2320, 3046
BsmBI CGTCTC 1 cut(s) 50
BsmFI GGGAC 3 cut(s) 1085, 2298, 3041
BsmI GAATGC 5 cut(s) 2164, 2544, 2601, 2655, 2712
Bso31I GGTCTC 3 cut(s) 63, 464, 2320
BsoBI CYCGRG 5 cut(s) 85, 280, 1529, 1702, 2436
Bsp1286I GDGCHC 7 cut(s) 519, 900, 1029, 1042, 2566, 2623, 3018
Bsp1407I TGTACA 2 cut(s) 1221, 1693
Bsp1720I GCTNAGC 2 cut(s) 1041, 2163
Bsp19I CCATGG 1 cut(s) 979
BspCNI CTCAG 8 cut(s) 595, 823, 955, 1054, 1729, 2155, 2566, 3411
BspFNI CGCG 5 cut(s) 564, 687, 810, 927, 2320
BspHI TCATGA 3 cut(s) 1264, 1291, 1450
BspMI ACCTGC 2 cut(s) 764, 3271
BspPI GGATC 7 cut(s) 1809, 1887, 1941, 1980, 2019, 2378, 2399
BspQI GCTCTTC 2 cut(s) 2571, 3518
BspTNI GGTCTC 3 cut(s) 63, 464, 2320
BsrBI CCGCTC 1 cut(s) 195
BsrDI GCAATG 6 cut(s) 603, 831, 963, 1246, 1944, 3418
BsrGI TGTACA 2 cut(s) 1221, 1693
BsrI ACTGG 7 cut(s) 351, 541, 1276, 1503, 1811, 2308, 2653
BssNI GRCGYC 2 cut(s) 1196, 2103
BssT1I CCWWGG 5 cut(s) 979, 1858, 1912, 2937, 3087
Bst2UI CCWGG 7 cut(s) 122, 1033, 1086, 1421, 1577, 2346, 2990
Bst6I CTCTTC 2 cut(s) 2571, 3518
BstACI GRCGYC 2 cut(s) 1196, 2103
BstAPI GCANNNNNTGC 2 cut(s) 779, 2948
BstAUI TGTACA 2 cut(s) 1221, 1693
BstC8I GCNNGC 7 cut(s) 203, 252, 1258, 1526, 2217, 2905, 2949
BstDSI CCRYGG 2 cut(s) 718, 979
BstEII GGTNACC 2 cut(s) 617, 1484
BstENI CCTNNNNNAGG 1 cut(s) 2941
BstFNI CGCG 5 cut(s) 564, 687, 810, 927, 2320
BstMAI GTCTC 7 cut(s) 50, 63, 464, 742, 1489, 2320, 3046
BstNI CCWGG 7 cut(s) 122, 1033, 1086, 1421, 1577, 2346, 2990
BstNSI RCATGY 3 cut(s) 1723, 2270, 2951
BstPI GGTNACC 2 cut(s) 617, 1484
BstSFI CTRYAG 1 cut(s) 3147
BstSLI GKGCMC 1 cut(s) 1029
BstUI CGCG 5 cut(s) 564, 687, 810, 927, 2320
BstV2I GAAGAC 7 cut(s) 30, 150, 516, 1176, 2784, 2806, 3245
BstX2I RGATCY 1 cut(s) 2404
BstYI RGATCY 1 cut(s) 2404
Bsu36I CCTNAGG 1 cut(s) 2553
BsuI GTATCC 1 cut(s) 2256
BtgI CCRYGG 2 cut(s) 718, 979
BtgZI GCGATG 1 cut(s) 467
BtrI CACGTC 1 cut(s) 432
BtsI GCAGTG 2 cut(s) 1881, 2356
BtsIMutI CAGTG 8 cut(s) 218, 242, 1804, 1881, 1890, 2132, 2356, 2485
BveI ACCTGC 2 cut(s) 764, 3271
Cac8I GCNNGC 7 cut(s) 203, 252, 1258, 1526, 2217, 2905, 2949
CciI TCATGA 3 cut(s) 1264, 1291, 1450
CseI GACGC 4 cut(s) 193, 194, 1204, 2092
Csp6I GTAC 7 cut(s) 82, 136, 153, 1222, 1694, 3033, 3250
CspCI CAANNNNNGTGG 4 cut(s) 313, 348, 757, 792
CviQI GTAC 7 cut(s) 82, 136, 153, 1222, 1694, 3033, 3250
DraIII CACNNNGTG 3 cut(s) 893, 3011, 3054
EaeI YGGCCR 1 cut(s) 976
Eam1104I CTCTTC 2 cut(s) 2571, 3518
EarI CTCTTC 2 cut(s) 2571, 3518
EciI GGCGGA 2 cut(s) 384, 3383
Ecl136II GAGCTC 1 cut(s) 898
Eco130I CCWWGG 5 cut(s) 979, 1858, 1912, 2937, 3087
Eco147I AGGCCT 1 cut(s) 1180
Eco24I GRGCYC 3 cut(s) 519, 900, 1042
Eco31I GGTCTC 3 cut(s) 63, 464, 2320
Eco47I GGWCC 7 cut(s) 106, 388, 478, 1282, 2149, 2285, 3103
Eco53kI GAGCTC 1 cut(s) 898
Eco57I CTGAAG 6 cut(s) 1411, 2461, 2517, 2796, 3116, 3186
Eco81I CCTNAGG 1 cut(s) 2553
Eco88I CYCGRG 5 cut(s) 85, 280, 1529, 1702, 2436
Eco91I GGTNACC 2 cut(s) 617, 1484
EcoICRI GAGCTC 1 cut(s) 898
EcoNI CCTNNNNNAGG 1 cut(s) 2941
EcoO109I RGGNCCY 5 cut(s) 836, 968, 2285, 2369, 3103
EcoO65I GGTNACC 2 cut(s) 617, 1484
EcoRI GAATTC 3 cut(s) 2182, 2203, 2508
EcoRII CCWGG 7 cut(s) 120, 1031, 1084, 1419, 1575, 2344, 2988
EcoT14I CCWWGG 5 cut(s) 979, 1858, 1912, 2937, 3087
EcoT38I GRGCYC 3 cut(s) 519, 900, 1042
ErhI CCWWGG 5 cut(s) 979, 1858, 1912, 2937, 3087
Esp3I CGTCTC 1 cut(s) 50
FalI AAGNNNNNCTT 2 cut(s) 3497, 3529
FaqI GGGAC 3 cut(s) 1085, 2298, 3041
FauI CCCGC 3 cut(s) 22, 630, 680
FauNDI CATATG 2 cut(s) 496, 1477
FbaI TGATCA 3 cut(s) 415, 2026, 2095
FblI GTMKAC 1 cut(s) 765
FriOI GRGCYC 3 cut(s) 519, 900, 1042
FspBI CTAG 6 cut(s) 627, 1490, 2259, 3051, 3107, 3200
GsaI CCCAGC 1 cut(s) 2541
GsuI CTGGAG 3 cut(s) 1986, 1987, 2136
HapII CCGG 6 cut(s) 127, 269, 293, 328, 1405, 1641
HgaI GACGC 4 cut(s) 193, 194, 1204, 2092
Hin1I GRCGYC 2 cut(s) 1196, 2103
HincII GTYRAC 2 cut(s) 1717, 1728
HindII GTYRAC 2 cut(s) 1717, 1728
HinfI GANTC 8 cut(s) 215, 259, 265, 1048, 1172, 1211, 2780, 3083
HpaII CCGG 6 cut(s) 127, 269, 293, 328, 1405, 1641
HphI GGTGA 8 cut(s) 65, 611, 905, 1121, 2334, 2708, 3298, 3532
Hpy166II GTNNAC 9 cut(s) 388, 429, 646, 766, 1027, 1222, 1343, 1717, 1728
Hpy8I GTNNAC 9 cut(s) 388, 429, 646, 766, 1027, 1222, 1343, 1717, 1728
Hpy99I CGWCG 5 cut(s) 53, 64, 78, 181, 436
HpyCH4IV ACGT 2 cut(s) 76, 431
HpySE526I ACGT 2 cut(s) 76, 431
Hsp92I GRCGYC 2 cut(s) 1196, 2103
Ksp22I TGATCA 3 cut(s) 415, 2026, 2095
LguI GCTCTTC 2 cut(s) 2571, 3518
LmnI GCTCC 6 cut(s) 903, 1253, 1630, 1816, 2275, 2541
LweI GCATC 8 cut(s) 791, 1108, 1589, 2228, 2349, 2518, 2632, 3067
MaeI CTAG 6 cut(s) 627, 1490, 2259, 3051, 3107, 3200
MaeII ACGT 2 cut(s) 76, 431
MbiI CCGCTC 1 cut(s) 195
MfeI CAATTG 1 cut(s) 663
MflI RGATCY 1 cut(s) 2404
MhlI GDGCHC 7 cut(s) 519, 900, 1029, 1042, 2566, 2623, 3018
MlsI TGGCCA 1 cut(s) 978
MluI ACGCGT 1 cut(s) 2318
MluNI TGGCCA 1 cut(s) 978
MlyI GAGTC 3 cut(s) 1057, 1166, 2774
MmeI TCCRAC 4 cut(s) 300, 1144, 1231, 1260
Mox20I TGGCCA 1 cut(s) 978
MroXI GAANNNNTTC 3 cut(s) 1770, 3330, 3512
MscI TGGCCA 1 cut(s) 978
MseI TTAA 6 cut(s) 2634, 2688, 2703, 2793, 2820, 2829
MslI CAYNNNNRTG 3 cut(s) 888, 1992, 3284
Msp20I TGGCCA 1 cut(s) 978
MspA1I CMGCKG 3 cut(s) 2537, 2800, 3064
MspI CCGG 6 cut(s) 127, 269, 293, 328, 1405, 1641
MunI CAATTG 1 cut(s) 663
Mva1269I GAATGC 5 cut(s) 2164, 2544, 2601, 2655, 2712
MvaI CCWGG 7 cut(s) 122, 1033, 1086, 1421, 1577, 2346, 2990
MvnI CGCG 5 cut(s) 564, 687, 810, 927, 2320
NciI CCSGG 3 cut(s) 127, 270, 294
NcoI CCATGG 1 cut(s) 979
NdeI CATATG 2 cut(s) 496, 1477
NmuCI GTSAC 9 cut(s) 20, 499, 617, 1109, 1864, 1918, 1957, 2035, 2104
NspI RCATGY 3 cut(s) 1723, 2270, 2951
PaeI GCATGC 1 cut(s) 2951
PaeR7I CTCGAG 3 cut(s) 1529, 1702, 2436
PagI TCATGA 3 cut(s) 1264, 1291, 1450
PaqCI CACCTGC 1 cut(s) 764
PasI CCCWGGG 1 cut(s) 1420
PceI AGGCCT 1 cut(s) 1180
PciI ACATGT 1 cut(s) 2266
PciSI GCTCTTC 2 cut(s) 2571, 3518
PcsI WCGNNNNNNNCGW 2 cut(s) 440, 568
PctI GAATGC 5 cut(s) 2164, 2544, 2601, 2655, 2712
PdmI GAANNNNTTC 3 cut(s) 1770, 3330, 3512
PfeI GAWTC 5 cut(s) 215, 259, 265, 1211, 3083
PflMI CCANNNNNTGG 1 cut(s) 2969
PfoI TCCNGGA 1 cut(s) 1084
PleI GAGTC 3 cut(s) 1056, 1166, 2774
PpsI GAGTC 3 cut(s) 1056, 1166, 2774
PpuMI RGGWCCY 2 cut(s) 2285, 3103
PscI ACATGT 1 cut(s) 2266
Psp124BI GAGCTC 1 cut(s) 900
Psp5II RGGWCCY 2 cut(s) 2285, 3103
Psp6I CCWGG 7 cut(s) 120, 1031, 1084, 1419, 1575, 2344, 2988
PspEI GGTNACC 2 cut(s) 617, 1484
PspFI CCCAGC 1 cut(s) 2537
PspGI CCWGG 7 cut(s) 120, 1031, 1084, 1419, 1575, 2344, 2988
PspPPI RGGWCCY 2 cut(s) 2285, 3103
PspXI VCTCGAGB 2 cut(s) 1529, 1702
PsuI RGATCY 1 cut(s) 2404
PvuII CAGCTG 1 cut(s) 2800
RsaI GTAC 7 cut(s) 83, 137, 154, 1223, 1695, 3034, 3251
RsaNI GTAC 7 cut(s) 82, 136, 153, 1222, 1694, 3033, 3250
RseI CAYNNNNRTG 3 cut(s) 888, 1992, 3284
SacI GAGCTC 1 cut(s) 900
SapI GCTCTTC 2 cut(s) 2571, 3518
SaqAI TTAA 6 cut(s) 2634, 2688, 2703, 2793, 2820, 2829
ScaI AGTACT 1 cut(s) 137
SchI GAGTC 3 cut(s) 1057, 1166, 2774
SduI GDGCHC 7 cut(s) 519, 900, 1029, 1042, 2566, 2623, 3018
SfaNI GCATC 8 cut(s) 791, 1108, 1589, 2228, 2349, 2518, 2632, 3067
SfcI CTRYAG 1 cut(s) 3147
Sfr274I CTCGAG 3 cut(s) 1529, 1702, 2436
SinI GGWCC 7 cut(s) 106, 388, 478, 1282, 2149, 2285, 3103
SlaI CTCGAG 3 cut(s) 1529, 1702, 2436
SmiMI CAYNNNNRTG 3 cut(s) 888, 1992, 3284
SmlI CTYRAG 9 cut(s) 732, 864, 1174, 1529, 1702, 2436, 2610, 2664, 2721
SmoI CTYRAG 9 cut(s) 732, 864, 1174, 1529, 1702, 2436, 2610, 2664, 2721
SphI GCATGC 1 cut(s) 2951
SseBI AGGCCT 1 cut(s) 1180
SspMI CTAG 6 cut(s) 627, 1490, 2259, 3051, 3107, 3200
SstI GAGCTC 1 cut(s) 900
StuI AGGCCT 1 cut(s) 1180
StyI CCWWGG 5 cut(s) 979, 1858, 1912, 2937, 3087
TaiI ACGT 2 cut(s) 79, 434
TaqI TCGA 9 cut(s) 41, 224, 571, 1464, 1530, 1703, 1990, 2437, 2455
TaqII GACCGA 2 cut(s) 24, 87
TatI WGTACW 5 cut(s) 135, 1221, 1693, 3032, 3249
TauI GCSGC 5 cut(s) 198, 201, 567, 813, 2537
TfiI GAWTC 5 cut(s) 215, 259, 265, 1211, 3083
Tru1I TTAA 6 cut(s) 2634, 2688, 2703, 2793, 2820, 2829
Tru9I TTAA 6 cut(s) 2634, 2688, 2703, 2793, 2820, 2829
TscAI CASTG 8 cut(s) 225, 242, 1811, 1881, 1897, 2132, 2356, 2485
TseFI GTSAC 9 cut(s) 20, 499, 617, 1109, 1864, 1918, 1957, 2035, 2104
Tsp45I GTSAC 9 cut(s) 20, 499, 617, 1109, 1864, 1918, 1957, 2035, 2104
TspGWI ACGGA 5 cut(s) 77, 170, 1204, 1567, 2597
TspRI CASTG 8 cut(s) 225, 242, 1811, 1881, 1897, 2132, 2356, 2485
Van91I CCANNNNNTGG 1 cut(s) 2969
VneI GTGCAC 1 cut(s) 1025
VpaK11BI GGWCC 7 cut(s) 106, 388, 478, 1282, 2149, 2285, 3103
XagI CCTNNNNNAGG 1 cut(s) 2941
XapI RAATTY 3 cut(s) 2182, 2203, 2508
XbaI TCTAGA 1 cut(s) 3199
XceI RCATGY 3 cut(s) 1723, 2270, 2951
XcmI CCANNNNNNNNNTGG 1 cut(s) 1591
XhoI CTCGAG 3 cut(s) 1529, 1702, 2436
XmiI GTMKAC 1 cut(s) 765
XmnI GAANNNNTTC 3 cut(s) 1770, 3330, 3512
XspI CTAG 6 cut(s) 627, 1490, 2259, 3051, 3107, 3200
ZrmI AGTACT 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.