Rorug05G0290200

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0014702
Physical Location & Seq
Reverse (-)
33326242 .. 33326705
464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0290200.1

Sequence Viewer

Length: 312 bp
ATGGCCACCCTCGGCGGAATTCGCGACTCCCTTGCCGGCTCCGAGAACAACCACGAGACCGAATCCCTCGCTCGCTTCTCCATCGACGAGCATAACAAGAAGGAGAATGCTTTGCTGGAGTTTGTTCGAGTGGTGAAGGCTAAGGAAGAAGTGGTTGTCGGCACTCTGCACCATCTGATTGTGGAGGCCATCGACGGCGGCAAGAAGAAGCTTTATGAAGCCAAGGTCTGGGTCAAGCCTTGGTTGGGCTTTAAAGAAGTTCAGGAATTCAAGCACGTTGAGGATCCCGAAGCTCCATCTGAAACACCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

103

Amino Acids

11.58

Weight (kDa)

5.52

Isoelectric Point (pI)

28.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cystatin PF00031 15 - 93 4.9e-13 Cystatin domain
SQAPI PF16845 18 - 93 1.3e-18 Aspartic acid proteinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014702)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g29670
malus_domestica MD03G1136700.v1.1
prunus_persica Prupe.6G121100_v2.0.a1 Prupe.6G121100_v2.0.a1
pyrus_communis pycom03g09250
rosa_chinensis RchiOBHm_Chr5g0055031
rosa_laevigata RLG00000034958
rosa_multiflora Rmu_co8511567.1_g000001 Rmu_sc0000925.1_g000013
rosa_roxburghii Rroxscaffold_1G00025460
rosa_rugosa Rorug05G0290200 Rorug05G0290300
rosa_samantha Rh5AG360200 Rh5BG371200 Rh5CG393500 Rh5DG385000
rosa_wichuraiana Rw5G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 228
AccII CGCG 1 cut(s) 24
AciI CCGC 2 cut(s) 15, 198
AclWI GGATC 2 cut(s) 278, 291
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 18, 266
AfiI CCNNNNNNNGG 2 cut(s) 228, 245
AgsI TTSAA 1 cut(s) 271
AluBI AGCT 2 cut(s) 211, 293
AluI AGCT 2 cut(s) 211, 293
Alw26I GTCTC 1 cut(s) 50
AlwI GGATC 2 cut(s) 278, 291
AoxI GGCC 2 cut(s) 3, 186
ApoI RAATTY 2 cut(s) 18, 266
AsuHPI GGTGA 1 cut(s) 145
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 283
BauI CACGAG 1 cut(s) 53
BccI CCATC 4 cut(s) 89, 180, 197, 304
BceAI ACGGC 1 cut(s) 211
BcgI CGANNNNNNTGC 2 cut(s) 14, 48
BcoDI GTCTC 1 cut(s) 50
BisI GCNGC 1 cut(s) 199
BlsI GCNGC 1 cut(s) 200
BmiI GGNNCC 2 cut(s) 40, 285
BpmI CTGGAG 1 cut(s) 137
Bpu10I CCTNAGC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 50
BsaJI CCNNGG 3 cut(s) 10, 222, 239
Bsc4I CCNNNNNNNGG 2 cut(s) 228, 245
Bse118I RCCGGY 1 cut(s) 35
BseDI CCNNGG 3 cut(s) 10, 222, 239
BseLI CCNNNNNNNGG 2 cut(s) 228, 245
BsgI GTGCAG 1 cut(s) 152
Bsh1236I CGCG 1 cut(s) 24
BshFI GGCC 2 cut(s) 5, 188
BsiSI CCGG 1 cut(s) 36
BslI CCNNNNNNNGG 2 cut(s) 228, 245
BsmAI GTCTC 1 cut(s) 50
BsmI GAATGC 1 cut(s) 112
BsnI GGCC 2 cut(s) 5, 188
Bso31I GGTCTC 1 cut(s) 50
Bsp143I GATC 1 cut(s) 283
Bsp68I TCGCGA 1 cut(s) 24
BspACI CCGC 2 cut(s) 15, 198
BspANI GGCC 2 cut(s) 5, 188
BspFNI CGCG 1 cut(s) 24
BspLI GGNNCC 2 cut(s) 40, 285
BspPI GGATC 2 cut(s) 278, 291
BspTNI GGTCTC 1 cut(s) 50
BsrFI RCCGGY 1 cut(s) 35
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 3 cut(s) 10, 222, 239
BssMI GATC 1 cut(s) 283
BssSI CACGAG 1 cut(s) 53
BssT1I CCWWGG 2 cut(s) 222, 239
Bst2BI CACGAG 1 cut(s) 53
Bst4CI ACNGT 1 cut(s) 309
BstC8I GCNNGC 2 cut(s) 37, 73
BstDEI CTNAG 1 cut(s) 141
BstFNI CGCG 1 cut(s) 24
BstKTI GATC 1 cut(s) 286
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 1 cut(s) 283
BstMWI GCNNNNNNNGC 1 cut(s) 21
BstUI CGCG 1 cut(s) 24
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
BsuRI GGCC 2 cut(s) 5, 188
BtuMI TCGCGA 1 cut(s) 24
Cac8I GCNNGC 2 cut(s) 37, 73
Cfr10I RCCGGY 1 cut(s) 35
CviJI RGCY 9 cut(s) 5, 39, 140, 188, 211, 221, 238, 249, 293
CviKI_1 RGCY 9 cut(s) 5, 39, 140, 188, 211, 221, 238, 249, 293
DdeI CTNAG 1 cut(s) 141
DpnI GATC 1 cut(s) 285
DpnII GATC 1 cut(s) 283
DraI TTTAAA 1 cut(s) 253
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 30
Eco130I CCWWGG 2 cut(s) 222, 239
Eco31I GGTCTC 1 cut(s) 50
EcoRI GAATTC 2 cut(s) 18, 266
EcoT14I CCWWGG 2 cut(s) 222, 239
ErhI CCWWGG 2 cut(s) 222, 239
FaiI YATR 2 cut(s) 93, 216
Fnu4HI GCNGC 1 cut(s) 199
Fsp4HI GCNGC 1 cut(s) 199
GluI GCNGC 1 cut(s) 199
GsuI CTGGAG 1 cut(s) 137
HaeIII GGCC 2 cut(s) 5, 188
HapII CCGG 1 cut(s) 36
HindIII AAGCTT 1 cut(s) 209
HinfI GANTC 2 cut(s) 26, 62
HpaII CCGG 1 cut(s) 36
HphI GGTGA 1 cut(s) 145
Hpy188I TCNGA 3 cut(s) 43, 177, 301
Hpy188III TCNNGA 3 cut(s) 23, 263, 287
Hpy99I CGWCG 2 cut(s) 89, 197
HpyAV CCTTC 2 cut(s) 94, 130
HpyCH4III ACNGT 1 cut(s) 309
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 1 cut(s) 169
HpyF10VI GCNNNNNNNGC 1 cut(s) 21
HpyF3I CTNAG 1 cut(s) 141
HpySE526I ACGT 1 cut(s) 276
KroI GCCGGC 1 cut(s) 35
KroNI GCCGGC 1 cut(s) 37
Kzo9I GATC 1 cut(s) 283
LmnI GCTCC 2 cut(s) 44, 298
LpnPI CCDG 4 cut(s) 49, 101, 214, 248
MaeII ACGT 1 cut(s) 276
MalI GATC 1 cut(s) 285
MboI GATC 1 cut(s) 283
MboII GAAGA 2 cut(s) 158, 217
MflI RGATCY 1 cut(s) 283
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 18, 266
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 20
MnlI CCTC 4 cut(s) 20, 77, 178, 274
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 35
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 252
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 36
Mva1269I GAATGC 1 cut(s) 112
MvnI CGCG 1 cut(s) 24
MwoI GCNNNNNNNGC 1 cut(s) 21
NaeI GCCGGC 1 cut(s) 37
NdeII GATC 1 cut(s) 283
NgoMIV GCCGGC 1 cut(s) 35
NlaIV GGNNCC 2 cut(s) 40, 285
NruI TCGCGA 1 cut(s) 24
PctI GAATGC 1 cut(s) 112
PdiI GCCGGC 1 cut(s) 37
PfeI GAWTC 1 cut(s) 62
PflMI CCANNNNNTGG 1 cut(s) 228
PkrI GCNGC 1 cut(s) 200
PleI GAGTC 1 cut(s) 20
PpsI GAGTC 1 cut(s) 20
PspN4I GGNNCC 2 cut(s) 40, 285
PsuI RGATCY 1 cut(s) 283
RruI TCGCGA 1 cut(s) 24
SaqAI TTAA 1 cut(s) 252
SatI GCNGC 1 cut(s) 199
Sau3AI GATC 1 cut(s) 283
SchI GAGTC 1 cut(s) 20
SetI ASST 4 cut(s) 213, 228, 279, 295
Sse9I AATT 2 cut(s) 18, 266
SsiI CCGC 2 cut(s) 15, 198
StyI CCWWGG 2 cut(s) 222, 239
TaaI ACNGT 1 cut(s) 309
TaiI ACGT 1 cut(s) 279
TaqI TCGA 3 cut(s) 84, 127, 192
TaqII GACCGA 1 cut(s) 74
TasI AATT 2 cut(s) 18, 266
TauI GCSGC 1 cut(s) 201
TfiI GAWTC 1 cut(s) 62
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TspDTI ATGAA 1 cut(s) 231
Van91I CCANNNNNTGG 1 cut(s) 228
XapI RAATTY 2 cut(s) 18, 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.