Rh5BG371200

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0014702
Physical Location & Seq
Forward (+)
58341851 .. 58347625
5775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG371200.1

Sequence Viewer

Length: 3549 bp
ATGAAGAAGCGGAGGTATTGTGACTTTGCGGGTCGGTCTTCGACTAACGACGGTGAGACGACCGAAGGAGGCCGACGTTCGTACTCGGGTCGGGTTTCGGGTTCGGACCAAACCGAAAGAGAGTACTTTGAAGACGACGGCGGTACGGATAGGCAACCGTTGAAACCGTCGCAGACGCATTTGAGCGGCGGCTTGCGTCAATGCGAATCACTGTCGATGAAGTTTCAGTGGAACAATCTGCTGGCCCAGAATCCGAATCCGGGTTCTAACCCGAGAAAGGTTCCGGGTCTGAACCGACCCGACTTGGATTATAGCAACCGGAGTGGTAGTTATAGACTGGATTATGGCGGAAGGGGTTTGGGTGTGGACCAGAGAAAGCAGTATGGGCATTTTGATCATCGTGGGTTCACGTCGCCATTGCCGAAATCCCATCTTGGTGGTCTCCAAGAAAACGAGGAGGACCACCGCCCAATCCCATATGCGACTCTGGAAGACGAGCCCAACTTTACTTCTGGAACTGGGTATCCATCAATGGAGGGTTTTCGCGGCGTTCGAGATGAATATGACAGAAGCAGAGCAATGGCTGGGTTGGCCATAGGTCACCCGCCTAGACAGAATGAGTTGGTGGAATATGGAGGTTCATCAATTGCTTTGAGTGAGGGTTTTCGCGGGGAGTTCATTAGAAGCAGAGAAATGGGCCATGGTGGATATTCTCAAGGTCAAACAGCGAGACACGATGAGTTGGTGGACAATGCAGGTGGTGCATCAGAAGCTTTGAATGAGGGTTTTCGCGGCAGAAGCAGAGCAATGGCTGAGGGGCCTGTTGGCAGCCATGCTGGATATACTCAAGGACTTACGCCAAGACACTCACATGGTGAACTCCCATTTGCTTTGAATGAGGGTTTCCGCGGTTCGGATGAGTTTAACAAAAGCAGAGCAATGGCTGAGGGGCCTATTGGCCATGGTGGATATTCTCATGGTCAAACACCAAGAAAGAATGAGTTGGTGCACCCCAGGTGGGCTCAGCAGAGTCCGAAAGAGGAAATGGGTAGGGACTACAAGGTTCCTGGAATGAAAAGAAATGACAATGGTGACATGGATGCCTTGTTCCTTCTTGACTTGCTCTATAAAAAGATGGCCACTGTTGGAGAAGACTCAAGGCCTCCAAGTCATTTGACGCCAAGCGTTGTTGATTCCGTTGTGTACAGGGTTGGTGGTAGCAATGGCTCTGTTGGAGCAGGCACTCATGAGAATTGCCAGTTGGACCAATATCATGATTATATGGAACCAACAAATGTTTTCCCCAACCATCAATCTACAAGTGAACCATCAATCCCCTGCTATGATGAAGTTTTGGGTTCATCTAATTCAAGAACAGAGGTGTGCCGGAACAATGAAACCCAGGGCTTCAGATTAGGAGTAGATGTTGGTCACGAAAGAGAGGTCGAGCCTTTATCATATGAAGGTAACCTAGAGAGACTGGCTCTGCCTGATGAAGATCAAGGCTTGCTCGAGGCTTATGGTGGTTGCCAACAGAGGTTGGCAGCAGCCGAAAGCCTGGCATCCATGACCCCTCTGGGAAGGTGGGCATTGAGAAAAGTTGCCATGAAGGAGCAACAATCCGGCAATCACTCAAAGTCTAAGTTCTTCCCTGATTTGAACCCCTCTTGTAATGTACAGAACTCGAGCTATGAAGTTGACATGCAGTTGACTACTACTGAGGATATGCAGCAGCAATATGCTAACAAAGATATTCAACAATATGCTGCTACCTGTGGAGAGGATCACTGGAACATGGAGCAAAATGTGATCTGTGGAGATGGTCAATGGAACATTGACCAAGGTGTGACCCGTGCAGTGGATCAAAGGAACACTGAACAAGGTTCCATTGACCAAGGTGTGACCTGTGGAGTGGATCAAAGGAACATTGCACAAGTTGTGACCTCTGGAGAGGATCAATGTAACATCCATCGAGGTGTGATCTCCAGAGGGGATCAATGGAACAATGATCAAGGTGTGACAACCTATGGAGAGCAACAATGGCGTATGGACAATTTAGGGGAACAATGGAACAATGATCAAGGCGTCACAACCTCTGGAGAGCAACAGTGGCGTATGAACTATGGAGAGGACCAATGGAATGCTGAGCATATCAACAACCTGAATTCTGACAGTTTGTTACCGAATTCTGAACTGCCAGCATCTACGCAAGAGCATTATGGCGAACAATCCAAATCTGCTAGGATACATGTAAAGGAGCGTTTGGGACCTCCCAAATTATCAACTGGAAGCATAAAAACGTGTTTGAGACCACCTATGAAATCACCTGGCAGTGTGAAGATGCGCTTGGGGCCTTCTGCAAATGATCCTAATATCAACATTTTAGATCCTAATATCAACATTTTAGGTTTCAAGCCTCGAGAACAATACAAATCTCGAATAAGAAAAACAGATGACTTCAGTGGAAGTATTCATCCTGAAGGGGATGGAATTCCAAAAGCTAAGACTGATGCTGCCGCTGGGAGCATTCATCCTCAGGCGAGTGCTCTTCCACAAGTCAAGACGGATGGTTTTATTGGAAGCATTCATCCTCAAGGGGGTGCTCATTCAATAGTTAAGACAGATGCTACTGGAAGCATTCATCCTCAAGGGAATGGTATTCCAAATGTTAAGAAAGGTGATTTTAATGGAAGCATTCGTCCTCAAGGGGCTGGTCTTTCAAAAGTTGATGTGAGAGCTGCAAAGAAAGAACCGCCTGAAGACTCTAAGGAGTTTAAGCAGCTGGTAGATGATGCCTTCATTAAGTTCATTAACATTTTGATTGAGAACCAACCTCAAAGAAGGAAAATCATGGAAGGAAAAGGAGGAAAGTGTTGCGTGTGTGCCAGCAATTCCAAAGAGTTTGTAGAAACGGCAACCTTAGCAAGGCATGCCTTCTTGTCGCCAAAGGTTGGTTTCAAAGCAGAACACCTGGGCTTTCATAAAGCACTTTGTGTGCTCTTGGGATGGGACAGTACAGCAGTCTCAAACACTAGGTGGGTTCAGCGGAACTTGCCAGATGCAGAATCCTTGGCTCTGAAGGAGGACCTAGTTATCTGGCCTCCTGTTGTTGTCATACACAATAGTTCTGTAGCAGATAAAAATCCTGAAGATGAAAGGCTGACGGTAAATGTTGAAGTTCTAGAGGCTATTCTTACAAGTATAGGATTTGTTGGGAGGAAGACCAATATGTACTGTGGGAAACCTGCCGATGGGAGTGTCATGGTGGTGAAGTTTCAGCCTACATTTTCTGGTTTGCAAGAAGCAGAAAGGCTTCACAAGCTGTTTGCTGATAACAAACATGGGAAGGCTGACTGGCGGTATATCAATTCTGTTGGCCTCAGCAACAGCAGCAATGGAAAACAAGACTTAGAGGCTGATGAGGCGAATTGTCTATATGGTTATTTGGGAAATGCAGACGATTGGGATGAACTTGACTTGGGAACCAAGAAGCACTTCGGGGTGAAGAGCAAGAAACAAATCAAGGCTATTGCAAATGCTTCTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

1182

Amino Acids

130.26

Weight (kDa)

5.93

Isoelectric Point (pI)

39.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 1028 - 1156 2.7e-19 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014702)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g29670
malus_domestica MD03G1136700.v1.1
prunus_persica Prupe.6G121100_v2.0.a1 Prupe.6G121100_v2.0.a1
pyrus_communis pycom03g09250
rosa_chinensis RchiOBHm_Chr5g0055031
rosa_laevigata RLG00000034958
rosa_multiflora Rmu_co8511567.1_g000001 Rmu_sc0000925.1_g000013
rosa_roxburghii Rroxscaffold_1G00025460
rosa_rugosa Rorug05G0290200 Rorug05G0290300
rosa_samantha Rh5AG360200 Rh5BG371200 Rh5CG393500 Rh5DG385000
rosa_wichuraiana Rw5G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 746
Acc36I ACCTGC 2 cut(s) 746, 3253
AccB7I CCANNNNNTGG 1 cut(s) 2951
AccBSI CCGCTC 1 cut(s) 186
AccII CGCG 4 cut(s) 546, 669, 792, 909
AclWI GGATC 7 cut(s) 1791, 1869, 1923, 1962, 2001, 2360, 2381
AcoI YGGCCR 3 cut(s) 591, 958, 1137
AcsI RAATTY 3 cut(s) 2164, 2185, 2490
AcuI CTGAAG 6 cut(s) 1393, 2443, 2499, 2778, 3098, 3168
AcyI GRCGYC 2 cut(s) 1178, 2085
AdeI CACNNNGTG 3 cut(s) 875, 2993, 3036
AfaI GTAC 7 cut(s) 83, 125, 145, 1205, 1677, 3016, 3233
AfiI CCNNNNNNNGG 9 cut(s) 260, 277, 913, 1018, 1858, 2597, 2925, 2951, 3251
AflIII ACRYGT 2 cut(s) 2248, 2300
AjiI CACGTC 1 cut(s) 411
AjnI CCWGG 6 cut(s) 1013, 1066, 1401, 1557, 2326, 2970
AjuI GAANNNNNNNTTGG 6 cut(s) 1244, 1276, 2330, 2362, 3479, 3511
AloI GAACNNNNNNTCC 6 cut(s) 508, 540, 1091, 1123, 1317, 1349
AluBI AGCT 6 cut(s) 773, 1689, 2501, 2738, 2782, 3322
AluI AGCT 6 cut(s) 773, 1689, 2501, 2738, 2782, 3322
Alw21I GWGCWC 4 cut(s) 1011, 2548, 2605, 3000
Alw26I GTCTC 6 cut(s) 50, 446, 724, 1471, 2302, 3028
Alw44I GTGCAC 1 cut(s) 1007
AlwI GGATC 7 cut(s) 1791, 1869, 1923, 1962, 2001, 2360, 2381
Ama87I CYCGRG 5 cut(s) 85, 271, 1511, 1684, 2418
ApaLI GTGCAC 1 cut(s) 1007
ApoI RAATTY 3 cut(s) 2164, 2185, 2490
ArsI GACNNNNNNTTYG 2 cut(s) 1428, 1460
Asp700I GAANNNNTTC 2 cut(s) 3312, 3494
AspLEI GCGC 1 cut(s) 2346
AsuC2I CCSGG 2 cut(s) 261, 285
AsuHPI GGTGA 8 cut(s) 65, 593, 887, 1103, 2316, 2690, 3280, 3514
AvaI CYCGRG 5 cut(s) 85, 271, 1511, 1684, 2418
AvaII GGWCC 7 cut(s) 106, 367, 460, 1264, 2131, 2267, 3085
AxyI CCTNAGG 1 cut(s) 2535
BaeGI GKGCMC 1 cut(s) 1011
BalI TGGCCA 3 cut(s) 593, 960, 1139
BanII GRGCYC 2 cut(s) 501, 1024
BbsI GAAGAC 6 cut(s) 30, 138, 498, 1158, 2766, 3227
Bbv12I GWGCWC 4 cut(s) 1011, 2548, 2605, 3000
BbvCI CCTCAGC 3 cut(s) 813, 945, 3380
BceAI ACGGC 2 cut(s) 154, 2928
BciT130I CCWGG 6 cut(s) 1015, 1068, 1403, 1559, 2328, 2972
BciVI GTATCC 2 cut(s) 534, 2238
BclI TGATCA 3 cut(s) 394, 2008, 2077
BcnI CCSGG 2 cut(s) 261, 285
BcoDI GTCTC 6 cut(s) 50, 446, 724, 1471, 2302, 3028
BfaI CTAG 6 cut(s) 609, 1472, 2241, 3033, 3089, 3182
BfmI CTRYAG 1 cut(s) 3129
BfuAI ACCTGC 2 cut(s) 746, 3253
BfuI GTATCC 2 cut(s) 534, 2238
BlpI GCTNAGC 2 cut(s) 1023, 2145
BmcAI AGTACT 1 cut(s) 125
Bme1390I CCNGG 8 cut(s) 261, 285, 1015, 1068, 1403, 1559, 2328, 2972
Bme18I GGWCC 7 cut(s) 106, 367, 460, 1264, 2131, 2267, 3085
BmeT110I CYCGRG 5 cut(s) 85, 271, 1511, 1684, 2418
BmgBI CACGTC 1 cut(s) 411
BmiI GGNNCC 9 cut(s) 282, 819, 951, 1065, 1287, 1885, 2268, 2352, 3484
BmrFI CCNGG 8 cut(s) 261, 285, 1015, 1068, 1403, 1559, 2328, 2972
BmrI ACTGGG 1 cut(s) 528
BmsI GCATC 9 cut(s) 773, 1090, 1571, 2210, 2331, 2500, 2614, 2782, 3049
BmuI ACTGGG 1 cut(s) 528
BpiI GAAGAC 6 cut(s) 30, 138, 498, 1158, 2766, 3227
BplI GAGNNNNNCTC 2 cut(s) 1468, 1500
BpmI CTGGAG 3 cut(s) 1968, 1969, 2118
Bpu10I CCTNAGC 4 cut(s) 813, 945, 2920, 3380
Bpu1102I GCTNAGC 2 cut(s) 1023, 2145
BpuEI CTTGAG 6 cut(s) 699, 831, 1141, 2577, 2631, 2688
BpuMI CCSGG 2 cut(s) 261, 285
BsaBI GATNNNNATC 2 cut(s) 1497, 3141
BsaHI GRCGYC 2 cut(s) 1178, 2085
BsaI GGTCTC 2 cut(s) 446, 2302
BsaWI WCCGGW 1 cut(s) 318
BsaXI ACNNNNNCTCC 5 cut(s) 34, 313, 343, 1410, 1440
Bsc4I CCNNNNNNNGG 9 cut(s) 260, 277, 913, 1018, 1858, 2597, 2925, 2951, 3251
Bse1I ACTGG 8 cut(s) 342, 523, 1258, 1485, 1793, 2290, 2635, 3359
Bse21I CCTNAGG 1 cut(s) 2535
Bse3DI GCAATG 7 cut(s) 416, 585, 813, 945, 1228, 1926, 3400
Bse8I GATNNNNATC 2 cut(s) 1497, 3141
BseBI CCWGG 6 cut(s) 1015, 1068, 1403, 1559, 2328, 2972
BseJI GATNNNNATC 2 cut(s) 1497, 3141
BseLI CCNNNNNNNGG 9 cut(s) 260, 277, 913, 1018, 1858, 2597, 2925, 2951, 3251
BseMI GCAATG 7 cut(s) 416, 585, 813, 945, 1228, 1926, 3400
BseMII CTCAG 7 cut(s) 804, 936, 1037, 1710, 2136, 2549, 3394
BseNI ACTGG 8 cut(s) 342, 523, 1258, 1485, 1793, 2290, 2635, 3359
BseRI GAGGAG 1 cut(s) 470
BseSI GKGCMC 1 cut(s) 1011
BseYI CCCAGC 2 cut(s) 584, 2519
BsgI GTGCAG 1 cut(s) 1875
Bsh1236I CGCG 4 cut(s) 546, 669, 792, 909
Bsh1285I CGRYCG 1 cut(s) 63
BsiEI CGRYCG 1 cut(s) 63
BsiHKAI GWGCWC 4 cut(s) 1011, 2548, 2605, 3000
BsiHKCI CYCGRG 5 cut(s) 85, 271, 1511, 1684, 2418
BsiSI CCGG 5 cut(s) 260, 284, 319, 1387, 1623
BslFI GGGAC 3 cut(s) 1067, 2280, 3023
BslI CCNNNNNNNGG 9 cut(s) 260, 277, 913, 1018, 1858, 2597, 2925, 2951, 3251
BsmAI GTCTC 6 cut(s) 50, 446, 724, 1471, 2302, 3028
BsmBI CGTCTC 1 cut(s) 50
BsmFI GGGAC 3 cut(s) 1067, 2280, 3023
BsmI GAATGC 5 cut(s) 2146, 2526, 2583, 2637, 2694
Bso31I GGTCTC 2 cut(s) 446, 2302
BsoBI CYCGRG 5 cut(s) 85, 271, 1511, 1684, 2418
Bsp1286I GDGCHC 6 cut(s) 501, 1011, 1024, 2548, 2605, 3000
Bsp1407I TGTACA 2 cut(s) 1203, 1675
Bsp1720I GCTNAGC 2 cut(s) 1023, 2145
Bsp19I CCATGG 2 cut(s) 700, 961
BspCNI CTCAG 7 cut(s) 805, 937, 1036, 1711, 2137, 2548, 3393
BspFNI CGCG 4 cut(s) 546, 669, 792, 909
BspHI TCATGA 2 cut(s) 1246, 1273
BspLI GGNNCC 9 cut(s) 282, 819, 951, 1065, 1287, 1885, 2268, 2352, 3484
BspMI ACCTGC 2 cut(s) 746, 3253
BspPI GGATC 7 cut(s) 1791, 1869, 1923, 1962, 2001, 2360, 2381
BspQI GCTCTTC 2 cut(s) 2553, 3500
BspTNI GGTCTC 2 cut(s) 446, 2302
BsrBI CCGCTC 1 cut(s) 186
BsrDI GCAATG 7 cut(s) 416, 585, 813, 945, 1228, 1926, 3400
BsrGI TGTACA 2 cut(s) 1203, 1675
BsrI ACTGG 8 cut(s) 342, 523, 1258, 1485, 1793, 2290, 2635, 3359
BssNI GRCGYC 2 cut(s) 1178, 2085
BssT1I CCWWGG 5 cut(s) 700, 961, 1840, 1894, 3069
Bst2UI CCWGG 6 cut(s) 1015, 1068, 1403, 1559, 2328, 2972
Bst6I CTCTTC 2 cut(s) 2553, 3500
BstACI GRCGYC 2 cut(s) 1178, 2085
BstAPI GCANNNNNTGC 2 cut(s) 761, 2930
BstAUI TGTACA 2 cut(s) 1203, 1675
BstC8I GCNNGC 7 cut(s) 194, 243, 1240, 1508, 2199, 2887, 2931
BstDSI CCRYGG 3 cut(s) 700, 907, 961
BstEII GGTNACC 2 cut(s) 599, 1466
BstENI CCTNNNNNAGG 1 cut(s) 2923
BstFNI CGCG 4 cut(s) 546, 669, 792, 909
BstHHI GCGC 1 cut(s) 2346
BstMAI GTCTC 6 cut(s) 50, 446, 724, 1471, 2302, 3028
BstMCI CGRYCG 1 cut(s) 63
BstNI CCWGG 6 cut(s) 1015, 1068, 1403, 1559, 2328, 2972
BstNSI RCATGY 3 cut(s) 1705, 2252, 2933
BstPI GGTNACC 2 cut(s) 599, 1466
BstSCI CCNGG 8 cut(s) 259, 283, 1013, 1066, 1401, 1557, 2326, 2970
BstSFI CTRYAG 1 cut(s) 3129
BstSLI GKGCMC 1 cut(s) 1011
BstUI CGCG 4 cut(s) 546, 669, 792, 909
BstV2I GAAGAC 6 cut(s) 30, 138, 498, 1158, 2766, 3227
BstX2I RGATCY 1 cut(s) 2386
BstXI CCANNNNNNTGG 1 cut(s) 437
BstYI RGATCY 1 cut(s) 2386
Bsu36I CCTNAGG 1 cut(s) 2535
BsuI GTATCC 2 cut(s) 534, 2238
BtgI CCRYGG 3 cut(s) 700, 907, 961
BtrI CACGTC 1 cut(s) 411
BtsI GCAGTG 2 cut(s) 1863, 2338
BtsIMutI CAGTG 9 cut(s) 209, 233, 1140, 1786, 1863, 1872, 2114, 2338, 2467
BveI ACCTGC 2 cut(s) 746, 3253
Cac8I GCNNGC 7 cut(s) 194, 243, 1240, 1508, 2199, 2887, 2931
CciI TCATGA 2 cut(s) 1246, 1273
CfoI GCGC 1 cut(s) 2346
Cfr42I CCGCGG 1 cut(s) 910
CseI GACGC 4 cut(s) 184, 185, 1186, 2074
Csp6I GTAC 7 cut(s) 82, 124, 144, 1204, 1676, 3015, 3232
CspCI CAANNNNNGTGG 4 cut(s) 304, 339, 739, 774
CviQI GTAC 7 cut(s) 82, 124, 144, 1204, 1676, 3015, 3232
DraIII CACNNNGTG 3 cut(s) 875, 2993, 3036
EaeI YGGCCR 3 cut(s) 591, 958, 1137
Eam1104I CTCTTC 2 cut(s) 2553, 3500
EarI CTCTTC 2 cut(s) 2553, 3500
EciI GGCGGA 1 cut(s) 363
Eco130I CCWWGG 5 cut(s) 700, 961, 1840, 1894, 3069
Eco147I AGGCCT 1 cut(s) 1162
Eco24I GRGCYC 2 cut(s) 501, 1024
Eco31I GGTCTC 2 cut(s) 446, 2302
Eco47I GGWCC 7 cut(s) 106, 367, 460, 1264, 2131, 2267, 3085
Eco57I CTGAAG 6 cut(s) 1393, 2443, 2499, 2778, 3098, 3168
Eco81I CCTNAGG 1 cut(s) 2535
Eco88I CYCGRG 5 cut(s) 85, 271, 1511, 1684, 2418
Eco91I GGTNACC 2 cut(s) 599, 1466
EcoNI CCTNNNNNAGG 1 cut(s) 2923
EcoO109I RGGNCCY 5 cut(s) 818, 950, 2267, 2351, 3085
EcoO65I GGTNACC 2 cut(s) 599, 1466
EcoRI GAATTC 3 cut(s) 2164, 2185, 2490
EcoRII CCWGG 6 cut(s) 1013, 1066, 1401, 1557, 2326, 2970
EcoT14I CCWWGG 5 cut(s) 700, 961, 1840, 1894, 3069
EcoT38I GRGCYC 2 cut(s) 501, 1024
ErhI CCWWGG 5 cut(s) 700, 961, 1840, 1894, 3069
Esp3I CGTCTC 1 cut(s) 50
FalI AAGNNNNNCTT 4 cut(s) 2330, 2362, 3479, 3511
FaqI GGGAC 3 cut(s) 1067, 2280, 3023
FauI CCCGC 3 cut(s) 22, 612, 662
FauNDI CATATG 2 cut(s) 478, 1459
FbaI TGATCA 3 cut(s) 394, 2008, 2077
FriOI GRGCYC 2 cut(s) 501, 1024
FspBI CTAG 6 cut(s) 609, 1472, 2241, 3033, 3089, 3182
GlaI GCGC 1 cut(s) 2345
GsaI CCCAGC 2 cut(s) 588, 2523
GsuI CTGGAG 3 cut(s) 1968, 1969, 2118
HapII CCGG 5 cut(s) 260, 284, 319, 1387, 1623
HgaI GACGC 4 cut(s) 184, 185, 1186, 2074
HhaI GCGC 1 cut(s) 2346
Hin1I GRCGYC 2 cut(s) 1178, 2085
Hin6I GCGC 1 cut(s) 2344
HinP1I GCGC 1 cut(s) 2344
HincII GTYRAC 2 cut(s) 1699, 1710
HindII GTYRAC 2 cut(s) 1699, 1710
HindIII AAGCTT 1 cut(s) 771
HinfI GANTC 9 cut(s) 206, 250, 256, 484, 1030, 1154, 1193, 2762, 3065
HpaII CCGG 5 cut(s) 260, 284, 319, 1387, 1623
HphI GGTGA 8 cut(s) 65, 593, 887, 1103, 2316, 2690, 3280, 3514
Hpy166II GTNNAC 9 cut(s) 367, 408, 748, 878, 1009, 1204, 1325, 1699, 1710
Hpy8I GTNNAC 9 cut(s) 367, 408, 748, 878, 1009, 1204, 1325, 1699, 1710
Hpy99I CGWCG 5 cut(s) 53, 78, 140, 172, 415
HpyCH4IV ACGT 3 cut(s) 76, 410, 2300
HpySE526I ACGT 3 cut(s) 76, 410, 2300
Hsp92I GRCGYC 2 cut(s) 1178, 2085
HspAI GCGC 1 cut(s) 2344
Ksp22I TGATCA 3 cut(s) 394, 2008, 2077
KspI CCGCGG 1 cut(s) 910
LguI GCTCTTC 2 cut(s) 2553, 3500
LmnI GCTCC 5 cut(s) 1235, 1612, 1798, 2257, 2523
LweI GCATC 9 cut(s) 773, 1090, 1571, 2210, 2331, 2500, 2614, 2782, 3049
MaeI CTAG 6 cut(s) 609, 1472, 2241, 3033, 3089, 3182
MaeII ACGT 3 cut(s) 76, 410, 2300
MbiI CCGCTC 1 cut(s) 186
MfeI CAATTG 1 cut(s) 645
MflI RGATCY 1 cut(s) 2386
MhlI GDGCHC 6 cut(s) 501, 1011, 1024, 2548, 2605, 3000
MlsI TGGCCA 3 cut(s) 593, 960, 1139
MluNI TGGCCA 3 cut(s) 593, 960, 1139
MlyI GAGTC 4 cut(s) 478, 1039, 1148, 2756
MmeI TCCRAC 3 cut(s) 1126, 1213, 1242
Mox20I TGGCCA 3 cut(s) 593, 960, 1139
MroXI GAANNNNTTC 2 cut(s) 3312, 3494
MscI TGGCCA 3 cut(s) 593, 960, 1139
MseI TTAA 7 cut(s) 924, 2616, 2670, 2685, 2775, 2802, 2811
MslI CAYNNNNRTG 4 cut(s) 435, 870, 1974, 3266
Msp20I TGGCCA 3 cut(s) 593, 960, 1139
MspA1I CMGCKG 4 cut(s) 909, 2519, 2782, 3046
MspI CCGG 5 cut(s) 260, 284, 319, 1387, 1623
MspR9I CCNGG 8 cut(s) 261, 285, 1015, 1068, 1403, 1559, 2328, 2972
MunI CAATTG 1 cut(s) 645
Mva1269I GAATGC 5 cut(s) 2146, 2526, 2583, 2637, 2694
MvaI CCWGG 6 cut(s) 1015, 1068, 1403, 1559, 2328, 2972
MvnI CGCG 4 cut(s) 546, 669, 792, 909
NciI CCSGG 2 cut(s) 261, 285
NcoI CCATGG 2 cut(s) 700, 961
NdeI CATATG 2 cut(s) 478, 1459
NlaIV GGNNCC 9 cut(s) 282, 819, 951, 1065, 1287, 1885, 2268, 2352, 3484
NmuCI GTSAC 9 cut(s) 20, 599, 1091, 1430, 1846, 1900, 1939, 2017, 2086
NspI RCATGY 3 cut(s) 1705, 2252, 2933
PaeI GCATGC 1 cut(s) 2933
PaeR7I CTCGAG 3 cut(s) 1511, 1684, 2418
PagI TCATGA 2 cut(s) 1246, 1273
PaqCI CACCTGC 1 cut(s) 746
PasI CCCWGGG 1 cut(s) 1402
PceI AGGCCT 1 cut(s) 1162
PciI ACATGT 1 cut(s) 2248
PciSI GCTCTTC 2 cut(s) 2553, 3500
PcsI WCGNNNNNNNCGW 2 cut(s) 419, 550
PctI GAATGC 5 cut(s) 2146, 2526, 2583, 2637, 2694
PdmI GAANNNNTTC 2 cut(s) 3312, 3494
PfeI GAWTC 5 cut(s) 206, 250, 256, 1193, 3065
PflMI CCANNNNNTGG 1 cut(s) 2951
PfoI TCCNGGA 1 cut(s) 1066
PleI GAGTC 4 cut(s) 478, 1038, 1148, 2756
PpsI GAGTC 4 cut(s) 478, 1038, 1148, 2756
PpuMI RGGWCCY 2 cut(s) 2267, 3085
PscI ACATGT 1 cut(s) 2248
Psp5II RGGWCCY 2 cut(s) 2267, 3085
Psp6I CCWGG 6 cut(s) 1013, 1066, 1401, 1557, 2326, 2970
PspEI GGTNACC 2 cut(s) 599, 1466
PspFI CCCAGC 2 cut(s) 584, 2519
PspGI CCWGG 6 cut(s) 1013, 1066, 1401, 1557, 2326, 2970
PspN4I GGNNCC 9 cut(s) 282, 819, 951, 1065, 1287, 1885, 2268, 2352, 3484
PspPPI RGGWCCY 2 cut(s) 2267, 3085
PspXI VCTCGAGB 2 cut(s) 1511, 1684
PsuI RGATCY 1 cut(s) 2386
PvuII CAGCTG 1 cut(s) 2782
RsaI GTAC 7 cut(s) 83, 125, 145, 1205, 1677, 3016, 3233
RsaNI GTAC 7 cut(s) 82, 124, 144, 1204, 1676, 3015, 3232
RseI CAYNNNNRTG 4 cut(s) 435, 870, 1974, 3266
SacII CCGCGG 1 cut(s) 910
SapI GCTCTTC 2 cut(s) 2553, 3500
SaqAI TTAA 7 cut(s) 924, 2616, 2670, 2685, 2775, 2802, 2811
ScaI AGTACT 1 cut(s) 125
SchI GAGTC 4 cut(s) 478, 1039, 1148, 2756
ScrFI CCNGG 8 cut(s) 261, 285, 1015, 1068, 1403, 1559, 2328, 2972
SduI GDGCHC 6 cut(s) 501, 1011, 1024, 2548, 2605, 3000
SfaNI GCATC 9 cut(s) 773, 1090, 1571, 2210, 2331, 2500, 2614, 2782, 3049
SfcI CTRYAG 1 cut(s) 3129
Sfr274I CTCGAG 3 cut(s) 1511, 1684, 2418
Sfr303I CCGCGG 1 cut(s) 910
SgrBI CCGCGG 1 cut(s) 910
SinI GGWCC 7 cut(s) 106, 367, 460, 1264, 2131, 2267, 3085
SlaI CTCGAG 3 cut(s) 1511, 1684, 2418
SmiMI CAYNNNNRTG 4 cut(s) 435, 870, 1974, 3266
SmlI CTYRAG 9 cut(s) 714, 846, 1156, 1511, 1684, 2418, 2592, 2646, 2703
SmoI CTYRAG 9 cut(s) 714, 846, 1156, 1511, 1684, 2418, 2592, 2646, 2703
SphI GCATGC 1 cut(s) 2933
SseBI AGGCCT 1 cut(s) 1162
SspMI CTAG 6 cut(s) 609, 1472, 2241, 3033, 3089, 3182
StuI AGGCCT 1 cut(s) 1162
StyD4I CCNGG 8 cut(s) 259, 283, 1013, 1066, 1401, 1557, 2326, 2970
StyI CCWWGG 5 cut(s) 700, 961, 1840, 1894, 3069
TaiI ACGT 3 cut(s) 79, 413, 2303
TaqI TCGA 9 cut(s) 41, 215, 553, 1446, 1512, 1685, 1972, 2419, 2437
TaqII GACCGA 2 cut(s) 24, 77
TatI WGTACW 5 cut(s) 123, 1203, 1675, 3014, 3231
TauI GCSGC 5 cut(s) 189, 192, 549, 795, 2519
TfiI GAWTC 5 cut(s) 206, 250, 256, 1193, 3065
Tru1I TTAA 7 cut(s) 924, 2616, 2670, 2685, 2775, 2802, 2811
Tru9I TTAA 7 cut(s) 924, 2616, 2670, 2685, 2775, 2802, 2811
TscAI CASTG 9 cut(s) 216, 233, 1147, 1793, 1863, 1879, 2114, 2338, 2467
TseFI GTSAC 9 cut(s) 20, 599, 1091, 1430, 1846, 1900, 1939, 2017, 2086
Tsp45I GTSAC 9 cut(s) 20, 599, 1091, 1430, 1846, 1900, 1939, 2017, 2086
TspGWI ACGGA 3 cut(s) 161, 1186, 2579
TspRI CASTG 9 cut(s) 216, 233, 1147, 1793, 1863, 1879, 2114, 2338, 2467
Van91I CCANNNNNTGG 1 cut(s) 2951
VneI GTGCAC 1 cut(s) 1007
VpaK11BI GGWCC 7 cut(s) 106, 367, 460, 1264, 2131, 2267, 3085
XagI CCTNNNNNAGG 1 cut(s) 2923
XapI RAATTY 3 cut(s) 2164, 2185, 2490
XbaI TCTAGA 1 cut(s) 3181
XceI RCATGY 3 cut(s) 1705, 2252, 2933
XcmI CCANNNNNNNNNTGG 1 cut(s) 1573
XhoI CTCGAG 3 cut(s) 1511, 1684, 2418
XmnI GAANNNNTTC 2 cut(s) 3312, 3494
XspI CTAG 6 cut(s) 609, 1472, 2241, 3033, 3089, 3182
ZrmI AGTACT 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.