Rroxscaffold_1G00025460

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014702
Physical Location & Seq
Reverse (-)
31956185 .. 31962646
6462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00025460.1

Sequence Viewer

Length: 3537 bp
ATGAAGAAGCGGAGGTATTGTGACTTTGCGGGTCGGTCTTCGACTAACGACGGTGAGACAACGGAAGGAGACCGACGTTCGTACTCGGGTCGGGTTTCGGGTTCGGACCAAACCGAAAGAGAGTACTATGAAGACGTCGGCGATACAGATAGGCAACCGTTGAAACCGTCGCAGACACATTTGAGCGGCGGCTTGCGTCAATGCGAATCACTGTCGATGAAGTTTCAGTGGAAAAATCTGCTGGCCCAGACCCCGAATCCGGGTTCTAACCCGAGAAAGGTTCCGGGTCTGAACCGACCCGACTTGGATTATAGCAACCGGAGTGGTAGTTATAGACTGGATTATGAAATGGGTTATGGCGGAAGGGGTTTGGGTGTGGACCAGAGAAAGCAGTATGGGCATTTTGATCATCGTGGGTTCACGTCGCCGTTGCCGAAATCCCATCTTGGTCTCCAAGAAAACGAGGAGGACCATCGCCCAATCCCATATGCGACTCTAGAAGACGAGCCCAAGTTTACTTCTGGAACTGGGTATTCATCAATGGGTTTTAAGGAGGGTTTTCGCGGCGTTCGAGATGAATATGACAGAAGCAGAGCAATGGCTGAGTTGCCAATAGGTCACACGCCTAGACGGAATGAGTTGGTGGACTATGGAGGTTCATCAATTGCTTTGAGTGAGGGTTTTCGCGGCGAGTTCAACAGAAGCAGAGAAATGGGCCATGGTGGATGTTCTCAAGGTCAAACAGCGAGACACGATGAGTTGGTAGACAATGCAGGTGGTGCATCCGAAGCTTTGAATGAGGGTTTTCGCGGCAGAAGCAGAGCAATGGCTGAGGGGCCTGTTGGCAGCCATGCTGGATATTCTCAAGGACTTACACCAAGACTCTCACATGGGAGTTCACCAATTGCTTTGAATGAGGGTTTTCGCGGTTCGGATGAGTTTAACAAAAGCACAGCAATGGCTGAGGGGCCTATTGGCCATGGTGGATATTCTCATGGTCAAACACCAAGAAAGAATGAGTTGGTGCACCCCAGGTGGGCTCAGCAGAGTCCGAAAGAGGAAATGGGTAGGGACTACAAGGTTCCTGGAATGAAAAGAAATGACAATGGTGACATGGATGCCTTGTTCCTTCTTGACTTGCTCTATAAAAAGATGGCTACTGTTGGAGAAGACTCAAGGCCTCCAAGTCATTTGACGCCAAGCGTTGTTGATTCTGTTGTGTACAGGGTTGGCGGTAGCAATGGCTCTGTTGGAGCAGGCACTCATGAGAATTGCCAGTTGGACCAATATCATGATTATATGGAACCAACAAATGTTTTCCCCAACCATCAATCTACAAGTGAACCATCAATCCCCTGCTATGATGAAGTTTTGGGTTCTTCTAATTCAAGAACAGAGGTGTGCCGGAACAATGAAACCCAGGGCTTCAGATTAGGAGTAGATGTTGGTCATGAAAGAGAGGTCGAGCCTTTATCATATGAAGGTAACCTAGAGAGACTGGCTCTGCCTGATGTAGATCAAGGCTTGCTCGAGGCTTATGGTGGTTGCCAACAGAGGTTGGCAGCAGCCGAAAGCCTGGCATCCATGACCCCTCTGGGAAGGTGGGCATTGAGAAAAGTTGCCATGAAGGAGCAACAATCCGGCAATCACTCAAAGTCTAAGTTTTTCCCTGATTTGAACCCCTCTTGTAATGTACAGAACTCGAGCTATGAAGTTGACATGCAGTTGACTACTACTGAGGATATGCAGCAGCAATATGCTAACGAAGATATTCAACAATATGCTGCTACCTGTGGAGAGGATCACTGGAACATGGAGCAAAATGTGATCTGTGGAGATGGTCAATGGAACATTGACCAAGGTGTGACCCGTGCAGTGGATCAAAGGAACACTGAACAAGGTTCCATTGACCAAGGTGTGACCTGTGGAGTGGATCAAAGGAACATTGCACAAGGTGTGACCTCTGGAGAGGATCAATGTAACATCCATCGAGGTGTGATCTCCGTAGGGGATCAATGGAACAATGATCAAGGTGTGACAACCTATGGAGAGCAACAATGGCGTATGGACAATGGAGGGGAACAATGGAACAATGATCAAGGCGTCACAACCTCTGGAGAGCAACAGTGGCGTATGAACTATGGAGAGGACCAATGGAATGCTGAGCATATCAACAACCTGAATTCTGACAGTTTGTTACCGAATTCTGAACTGCCAGCATCTACGCAAGAGCATTATGGCGAACAATCCAAATCTGCTAGGATACATGTAAAGGAGCGTTTGGGACCTCCCAAATTATCAACTGGAAGCATAAAAACGCGTTTGAGACCACCTATGAAATCACCTGGCAGTGTGAAGATGCGTTTGGGGCCTTCTGCAAATGATCCTAATATCAACATTTTAGGTTTCAAGCCTCGAGAACAATACAAATCTCGAATAAGAAAAACAGATGACTTCAGTGGAAGTATTCATCCTGAAGGGGATGGAATTCCAAAAGCTAAGACTGATGCTGCTGCTGGGAGCATTCATCCTCAGGGGAGTGCTCTTCCACAAGTCAAGACGGATGGTTTTATTGGAAGCATTCATCCTCAAGGGGGTGGTCATTCAATAGTTAAGACAGATGCTACTGGAAGCATTCATCCTCAAGGGAATGGTATTCCAAATGTTAAGAAAGGTGATTTTAATGGAAGCATTTGTCCTCAAGGGGGTAGGCTTTCAAAAGTTGATGTGAAAGCTGCAAAGAAAGAACCGCCTGAAGACTCTAAGGAGTTTAAGCAGCTAGTAGAGGATGCCTTCATTAAGTTTGTTAACATTTTGATTGAGAACCCACCTCAAAGAAGGAAAATCATGGAAGGAAAAGGAGGAAAGTGTTGCGTGTGTGCCAGCAATTCCAAAGAGTTTGTAGAAACGGCAACCTTAGCAAGGCATGCCTTCTTGTCGCCAAAGGTTGGTTTCAAAGCAGAACACCTGGGCTTTCATAAAGCACTTTGTGTGGTCTTGGGATGGGACAGTACAGCAGTCTCAAACACTAGGTGGGTTCAGCGGAATTTGCCAGATGCAGAAACCTTGGCTCTGAAGGAGGACCTAGTTATCTGGCCTCCTGTTGTTGTCATACACAATAGTTCTGTAGCAAATAAAAATCCTGAAGATGAAAGGCTGACGGTAAATGTTGAAGTTCTAGAGGCTATTCTTACAAGTATAGGATTTATTGGGAGGAAGACCAATATGTACTGTGGGAAACCTGCCGATGGGAGTGTCATGGTGGTGAAGTTTCAGCCTACATTTTCTGGTTTGCTAGAAGCAGAAAGGCTTCACAAGCTGTTTGCTGATAACAAACATGGGAAGGCTGAGTGGCGGTATATCAATTCTGTTGGCCTCAGCAATGGAAAGCAAGACTTAGAGGCTGATGAGGCGAATTGTCTATATGGTTATTTGGGAAATGCGGACGATTGGGATGAACTCGACTTGGGAACCAAGAAGCACTTCGGGGTGAAGAGCAAGAAACAAATCAAGGCTATTGCAAATGCTTCTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

1178

Amino Acids

129.76

Weight (kDa)

5.98

Isoelectric Point (pI)

38.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 1027 - 1152 1e-18 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014702)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g29670
malus_domestica MD03G1136700.v1.1
prunus_persica Prupe.6G121100_v2.0.a1 Prupe.6G121100_v2.0.a1
pyrus_communis pycom03g09250
rosa_chinensis RchiOBHm_Chr5g0055031
rosa_laevigata RLG00000034958
rosa_multiflora Rmu_co8511567.1_g000001 Rmu_sc0000925.1_g000013
rosa_roxburghii Rroxscaffold_1G00025460
rosa_rugosa Rorug05G0290200 Rorug05G0290300
rosa_samantha Rh5AG360200 Rh5BG371200 Rh5CG393500 Rh5DG385000
rosa_wichuraiana Rw5G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 764
AatII GACGTC 1 cut(s) 138
Acc36I ACCTGC 2 cut(s) 764, 3250
AccB7I CCANNNNNTGG 1 cut(s) 2948
AccBSI CCGCTC 1 cut(s) 186
AccI GTMKAC 1 cut(s) 765
AccII CGCG 5 cut(s) 564, 687, 810, 927, 2320
AclWI GGATC 6 cut(s) 1809, 1887, 1941, 1980, 2019, 2378
AcoI YGGCCR 1 cut(s) 976
AcsI RAATTY 4 cut(s) 2182, 2203, 2487, 3046
AcuI CTGAAG 6 cut(s) 1411, 2440, 2496, 2775, 3095, 3165
AcyI GRCGYC 3 cut(s) 135, 1196, 2103
AdeI CACNNNGTG 3 cut(s) 1955, 2990, 3033
AfaI GTAC 6 cut(s) 83, 125, 1223, 1695, 3013, 3230
AflIII ACRYGT 2 cut(s) 2266, 2318
AjiI CACGTC 1 cut(s) 423
AjnI CCWGG 6 cut(s) 1031, 1084, 1419, 1575, 2344, 2967
AjuI GAANNNNNNNTTGG 6 cut(s) 1262, 1294, 2348, 2380, 3467, 3499
AloI GAACNNNNNNTCC 4 cut(s) 1109, 1141, 1335, 1367
AluBI AGCT 6 cut(s) 791, 1707, 2498, 2735, 2779, 3319
AluI AGCT 6 cut(s) 791, 1707, 2498, 2735, 2779, 3319
Alw21I GWGCWC 2 cut(s) 1029, 2545
Alw26I GTCTC 7 cut(s) 50, 63, 455, 742, 1489, 2320, 3025
Alw44I GTGCAC 1 cut(s) 1025
AlwI GGATC 6 cut(s) 1809, 1887, 1941, 1980, 2019, 2378
Ama87I CYCGRG 5 cut(s) 85, 271, 1529, 1702, 2415
AoxI GGCC 9 cut(s) 243, 715, 836, 968, 976, 1178, 2369, 3095, 3373
ApaLI GTGCAC 1 cut(s) 1025
ApoI RAATTY 4 cut(s) 2182, 2203, 2487, 3046
Asp700I GAANNNNTTC 3 cut(s) 1770, 3309, 3482
AsuC2I CCSGG 2 cut(s) 261, 285
AsuHPI GGTGA 7 cut(s) 65, 891, 1121, 2334, 2687, 3277, 3502
AvaI CYCGRG 5 cut(s) 85, 271, 1529, 1702, 2415
AvaII GGWCC 7 cut(s) 106, 379, 469, 1282, 2149, 2285, 3082
AxyI CCTNAGG 1 cut(s) 2532
BaeGI GKGCMC 1 cut(s) 1029
BalI TGGCCA 1 cut(s) 978
BanII GRGCYC 2 cut(s) 510, 1042
BbsI GAAGAC 6 cut(s) 30, 138, 507, 1176, 2763, 3224
Bbv12I GWGCWC 2 cut(s) 1029, 2545
BbvCI CCTCAGC 3 cut(s) 831, 963, 3377
BceAI ACGGC 2 cut(s) 412, 2925
BciT130I CCWGG 6 cut(s) 1033, 1086, 1421, 1577, 2346, 2969
BciVI GTATCC 1 cut(s) 2256
BclI TGATCA 3 cut(s) 406, 2026, 2095
BcnI CCSGG 2 cut(s) 261, 285
BcoDI GTCTC 7 cut(s) 50, 63, 455, 742, 1489, 2320, 3025
BfaI CTAG 9 cut(s) 497, 627, 1490, 2259, 2780, 3030, 3086, 3179, 3296
BfmI CTRYAG 1 cut(s) 3126
BfuAI ACCTGC 2 cut(s) 764, 3250
BfuI GTATCC 1 cut(s) 2256
BlpI GCTNAGC 2 cut(s) 1041, 2163
BmcAI AGTACT 1 cut(s) 125
Bme1390I CCNGG 8 cut(s) 261, 285, 1033, 1086, 1421, 1577, 2346, 2969
Bme18I GGWCC 7 cut(s) 106, 379, 469, 1282, 2149, 2285, 3082
BmeT110I CYCGRG 5 cut(s) 85, 271, 1529, 1702, 2415
BmgBI CACGTC 1 cut(s) 423
BmiI GGNNCC 9 cut(s) 282, 837, 969, 1083, 1305, 1903, 2286, 2370, 3472
BmrFI CCNGG 8 cut(s) 261, 285, 1033, 1086, 1421, 1577, 2346, 2969
BmrI ACTGGG 1 cut(s) 537
BmsI GCATC 9 cut(s) 791, 1108, 1589, 2228, 2349, 2497, 2611, 2779, 3046
BmuI ACTGGG 1 cut(s) 537
BpiI GAAGAC 6 cut(s) 30, 138, 507, 1176, 2763, 3224
BplI GAGNNNNNCTC 2 cut(s) 1486, 1518
BpmI CTGGAG 2 cut(s) 1986, 2136
Bpu10I CCTNAGC 4 cut(s) 831, 963, 2917, 3377
Bpu1102I GCTNAGC 2 cut(s) 1041, 2163
BpuEI CTTGAG 6 cut(s) 717, 849, 1159, 2574, 2628, 2685
BpuMI CCSGG 2 cut(s) 261, 285
BsaBI GATNNNNATC 1 cut(s) 1515
BsaHI GRCGYC 3 cut(s) 135, 1196, 2103
BsaI GGTCTC 3 cut(s) 63, 455, 2320
BsaJI CCNNGG 9 cut(s) 718, 979, 1031, 1419, 1420, 1858, 1912, 2968, 3066
BsaWI WCCGGW 1 cut(s) 318
BsaXI ACNNNNNCTCC 5 cut(s) 34, 313, 343, 1428, 1458
Bse1I ACTGG 7 cut(s) 342, 532, 1276, 1503, 1811, 2308, 2632
Bse21I CCTNAGG 1 cut(s) 2532
Bse3DI GCAATG 6 cut(s) 603, 831, 963, 1246, 1944, 3388
Bse8I GATNNNNATC 1 cut(s) 1515
BseBI CCWGG 6 cut(s) 1033, 1086, 1421, 1577, 2346, 2969
BseDI CCNNGG 9 cut(s) 718, 979, 1031, 1419, 1420, 1858, 1912, 2968, 3066
BseJI GATNNNNATC 1 cut(s) 1515
BseMI GCAATG 6 cut(s) 603, 831, 963, 1246, 1944, 3388
BseMII CTCAG 9 cut(s) 594, 822, 954, 1055, 1728, 2154, 2546, 3339, 3391
BseNI ACTGG 7 cut(s) 342, 532, 1276, 1503, 1811, 2308, 2632
BseRI GAGGAG 1 cut(s) 479
BseSI GKGCMC 1 cut(s) 1029
BseYI CCCAGC 1 cut(s) 2516
BsgI GTGCAG 1 cut(s) 1893
Bsh1236I CGCG 5 cut(s) 564, 687, 810, 927, 2320
BshFI GGCC 9 cut(s) 245, 717, 838, 970, 978, 1180, 2371, 3097, 3375
BsiHKAI GWGCWC 2 cut(s) 1029, 2545
BsiHKCI CYCGRG 5 cut(s) 85, 271, 1529, 1702, 2415
BsiSI CCGG 5 cut(s) 260, 284, 319, 1405, 1641
BslFI GGGAC 3 cut(s) 1085, 2298, 3020
BsmAI GTCTC 7 cut(s) 50, 63, 455, 742, 1489, 2320, 3025
BsmFI GGGAC 3 cut(s) 1085, 2298, 3020
BsmI GAATGC 4 cut(s) 2164, 2523, 2580, 2634
BsnI GGCC 9 cut(s) 245, 717, 838, 970, 978, 1180, 2371, 3097, 3375
Bso31I GGTCTC 3 cut(s) 63, 455, 2320
BsoBI CYCGRG 5 cut(s) 85, 271, 1529, 1702, 2415
Bsp1286I GDGCHC 4 cut(s) 510, 1029, 1042, 2545
Bsp1407I TGTACA 2 cut(s) 1221, 1693
Bsp1720I GCTNAGC 2 cut(s) 1041, 2163
Bsp19I CCATGG 2 cut(s) 718, 979
BspANI GGCC 9 cut(s) 245, 717, 838, 970, 978, 1180, 2371, 3097, 3375
BspCNI CTCAG 9 cut(s) 595, 823, 955, 1054, 1729, 2155, 2545, 3340, 3390
BspFNI CGCG 5 cut(s) 564, 687, 810, 927, 2320
BspHI TCATGA 3 cut(s) 1264, 1291, 1450
BspLI GGNNCC 9 cut(s) 282, 837, 969, 1083, 1305, 1903, 2286, 2370, 3472
BspMI ACCTGC 2 cut(s) 764, 3250
BspPI GGATC 6 cut(s) 1809, 1887, 1941, 1980, 2019, 2378
BspQI GCTCTTC 2 cut(s) 2550, 3488
BspTNI GGTCTC 3 cut(s) 63, 455, 2320
BsrBI CCGCTC 1 cut(s) 186
BsrDI GCAATG 6 cut(s) 603, 831, 963, 1246, 1944, 3388
BsrGI TGTACA 2 cut(s) 1221, 1693
BsrI ACTGG 7 cut(s) 342, 532, 1276, 1503, 1811, 2308, 2632
BssECI CCNNGG 9 cut(s) 718, 979, 1031, 1419, 1420, 1858, 1912, 2968, 3066
BssNI GRCGYC 3 cut(s) 135, 1196, 2103
BssT1I CCWWGG 5 cut(s) 718, 979, 1858, 1912, 3066
Bst2UI CCWGG 6 cut(s) 1033, 1086, 1421, 1577, 2346, 2969
Bst6I CTCTTC 2 cut(s) 2550, 3488
BstACI GRCGYC 3 cut(s) 135, 1196, 2103
BstAPI GCANNNNNTGC 2 cut(s) 779, 2927
BstAUI TGTACA 2 cut(s) 1221, 1693
BstC8I GCNNGC 7 cut(s) 194, 243, 1258, 1526, 2217, 2884, 2928
BstDSI CCRYGG 2 cut(s) 718, 979
BstEII GGTNACC 1 cut(s) 1484
BstENI CCTNNNNNAGG 1 cut(s) 2920
BstFNI CGCG 5 cut(s) 564, 687, 810, 927, 2320
BstMAI GTCTC 7 cut(s) 50, 63, 455, 742, 1489, 2320, 3025
BstNI CCWGG 6 cut(s) 1033, 1086, 1421, 1577, 2346, 2969
BstNSI RCATGY 3 cut(s) 1723, 2270, 2930
BstPI GGTNACC 1 cut(s) 1484
BstSCI CCNGG 8 cut(s) 259, 283, 1031, 1084, 1419, 1575, 2344, 2967
BstSFI CTRYAG 1 cut(s) 3126
BstSLI GKGCMC 1 cut(s) 1029
BstUI CGCG 5 cut(s) 564, 687, 810, 927, 2320
BstV2I GAAGAC 6 cut(s) 30, 138, 507, 1176, 2763, 3224
Bsu36I CCTNAGG 1 cut(s) 2532
BsuI GTATCC 1 cut(s) 2256
BsuRI GGCC 9 cut(s) 245, 717, 838, 970, 978, 1180, 2371, 3097, 3375
BtgI CCRYGG 2 cut(s) 718, 979
BtgZI GCGATG 1 cut(s) 458
BtrI CACGTC 1 cut(s) 423
BtsI GCAGTG 2 cut(s) 1881, 2356
BtsIMutI CAGTG 8 cut(s) 209, 233, 1804, 1881, 1890, 2132, 2356, 2464
BveI ACCTGC 2 cut(s) 764, 3250
Cac8I GCNNGC 7 cut(s) 194, 243, 1258, 1526, 2217, 2884, 2928
CciI TCATGA 3 cut(s) 1264, 1291, 1450
CseI GACGC 3 cut(s) 185, 1204, 2092
Csp6I GTAC 6 cut(s) 82, 124, 1222, 1694, 3012, 3229
CspCI CAANNNNNGTGG 4 cut(s) 304, 339, 757, 792
CviQI GTAC 6 cut(s) 82, 124, 1222, 1694, 3012, 3229
DraIII CACNNNGTG 3 cut(s) 1955, 2990, 3033
EaeI YGGCCR 1 cut(s) 976
Eam1104I CTCTTC 2 cut(s) 2550, 3488
EarI CTCTTC 2 cut(s) 2550, 3488
EciI GGCGGA 1 cut(s) 375
Eco130I CCWWGG 5 cut(s) 718, 979, 1858, 1912, 3066
Eco147I AGGCCT 1 cut(s) 1180
Eco24I GRGCYC 2 cut(s) 510, 1042
Eco31I GGTCTC 3 cut(s) 63, 455, 2320
Eco47I GGWCC 7 cut(s) 106, 379, 469, 1282, 2149, 2285, 3082
Eco57I CTGAAG 6 cut(s) 1411, 2440, 2496, 2775, 3095, 3165
Eco81I CCTNAGG 1 cut(s) 2532
Eco88I CYCGRG 5 cut(s) 85, 271, 1529, 1702, 2415
Eco91I GGTNACC 1 cut(s) 1484
EcoNI CCTNNNNNAGG 1 cut(s) 2920
EcoO109I RGGNCCY 5 cut(s) 836, 968, 2285, 2369, 3082
EcoO65I GGTNACC 1 cut(s) 1484
EcoRI GAATTC 3 cut(s) 2182, 2203, 2487
EcoRII CCWGG 6 cut(s) 1031, 1084, 1419, 1575, 2344, 2967
EcoT14I CCWWGG 5 cut(s) 718, 979, 1858, 1912, 3066
EcoT38I GRGCYC 2 cut(s) 510, 1042
ErhI CCWWGG 5 cut(s) 718, 979, 1858, 1912, 3066
FalI AAGNNNNNCTT 4 cut(s) 3380, 3412, 3467, 3499
FaqI GGGAC 3 cut(s) 1085, 2298, 3020
FauI CCCGC 1 cut(s) 22
FauNDI CATATG 2 cut(s) 487, 1477
FbaI TGATCA 3 cut(s) 406, 2026, 2095
FblI GTMKAC 1 cut(s) 765
FriOI GRGCYC 2 cut(s) 510, 1042
FspBI CTAG 9 cut(s) 497, 627, 1490, 2259, 2780, 3030, 3086, 3179, 3296
GsaI CCCAGC 1 cut(s) 2520
GsuI CTGGAG 2 cut(s) 1986, 2136
HaeIII GGCC 9 cut(s) 245, 717, 838, 970, 978, 1180, 2371, 3097, 3375
HapII CCGG 5 cut(s) 260, 284, 319, 1405, 1641
HgaI GACGC 3 cut(s) 185, 1204, 2092
Hin1I GRCGYC 3 cut(s) 135, 1196, 2103
HincII GTYRAC 3 cut(s) 1717, 1728, 2809
HindII GTYRAC 3 cut(s) 1717, 1728, 2809
HindIII AAGCTT 1 cut(s) 789
HinfI GANTC 8 cut(s) 206, 256, 493, 882, 1048, 1172, 1211, 2759
HpaI GTTAAC 1 cut(s) 2809
HpaII CCGG 5 cut(s) 260, 284, 319, 1405, 1641
HphI GGTGA 7 cut(s) 65, 891, 1121, 2334, 2687, 3277, 3502
Hpy188I TCNGA 9 cut(s) 106, 291, 787, 934, 1053, 1430, 2188, 2209, 3075
Hpy99I CGWCG 5 cut(s) 53, 78, 140, 172, 427
HpyCH4IV ACGT 3 cut(s) 76, 135, 422
HpySE526I ACGT 3 cut(s) 76, 135, 422
Hsp92I GRCGYC 3 cut(s) 135, 1196, 2103
Ksp22I TGATCA 3 cut(s) 406, 2026, 2095
KspAI GTTAAC 1 cut(s) 2809
LguI GCTCTTC 2 cut(s) 2550, 3488
LmnI GCTCC 5 cut(s) 1253, 1630, 1816, 2275, 2520
LweI GCATC 9 cut(s) 791, 1108, 1589, 2228, 2349, 2497, 2611, 2779, 3046
MaeI CTAG 9 cut(s) 497, 627, 1490, 2259, 2780, 3030, 3086, 3179, 3296
MaeII ACGT 3 cut(s) 76, 135, 422
MbiI CCGCTC 1 cut(s) 186
MfeI CAATTG 2 cut(s) 663, 903
MhlI GDGCHC 4 cut(s) 510, 1029, 1042, 2545
MlsI TGGCCA 1 cut(s) 978
MluI ACGCGT 1 cut(s) 2318
MluNI TGGCCA 1 cut(s) 978
MlyI GAGTC 5 cut(s) 487, 876, 1057, 1166, 2753
MmeI TCCRAC 3 cut(s) 1144, 1231, 1260
Mox20I TGGCCA 1 cut(s) 978
MroXI GAANNNNTTC 3 cut(s) 1770, 3309, 3482
MscI TGGCCA 1 cut(s) 978
MseI TTAA 8 cut(s) 549, 942, 2613, 2667, 2682, 2772, 2799, 2808
MslI CAYNNNNRTG 3 cut(s) 956, 1992, 3263
Msp20I TGGCCA 1 cut(s) 978
MspA1I CMGCKG 1 cut(s) 3043
MspI CCGG 5 cut(s) 260, 284, 319, 1405, 1641
MspR9I CCNGG 8 cut(s) 261, 285, 1033, 1086, 1421, 1577, 2346, 2969
MunI CAATTG 2 cut(s) 663, 903
Mva1269I GAATGC 4 cut(s) 2164, 2523, 2580, 2634
MvaI CCWGG 6 cut(s) 1033, 1086, 1421, 1577, 2346, 2969
MvnI CGCG 5 cut(s) 564, 687, 810, 927, 2320
NciI CCSGG 2 cut(s) 261, 285
NcoI CCATGG 2 cut(s) 718, 979
NdeI CATATG 2 cut(s) 487, 1477
NlaIV GGNNCC 9 cut(s) 282, 837, 969, 1083, 1305, 1903, 2286, 2370, 3472
NmuCI GTSAC 8 cut(s) 20, 617, 1109, 1864, 1918, 1957, 2035, 2104
NspI RCATGY 3 cut(s) 1723, 2270, 2930
PaeI GCATGC 1 cut(s) 2930
PaeR7I CTCGAG 3 cut(s) 1529, 1702, 2415
PagI TCATGA 3 cut(s) 1264, 1291, 1450
PaqCI CACCTGC 1 cut(s) 764
PasI CCCWGGG 1 cut(s) 1420
PceI AGGCCT 1 cut(s) 1180
PciI ACATGT 1 cut(s) 2266
PciSI GCTCTTC 2 cut(s) 2550, 3488
PcsI WCGNNNNNNNCGW 2 cut(s) 431, 568
PctI GAATGC 4 cut(s) 2164, 2523, 2580, 2634
PdmI GAANNNNTTC 3 cut(s) 1770, 3309, 3482
PfeI GAWTC 3 cut(s) 206, 256, 1211
PflMI CCANNNNNTGG 1 cut(s) 2948
PfoI TCCNGGA 1 cut(s) 1084
PleI GAGTC 5 cut(s) 487, 876, 1056, 1166, 2753
PpsI GAGTC 5 cut(s) 487, 876, 1056, 1166, 2753
PpuMI RGGWCCY 2 cut(s) 2285, 3082
PscI ACATGT 1 cut(s) 2266
Psp5II RGGWCCY 2 cut(s) 2285, 3082
Psp6I CCWGG 6 cut(s) 1031, 1084, 1419, 1575, 2344, 2967
PspEI GGTNACC 1 cut(s) 1484
PspFI CCCAGC 1 cut(s) 2516
PspGI CCWGG 6 cut(s) 1031, 1084, 1419, 1575, 2344, 2967
PspN4I GGNNCC 9 cut(s) 282, 837, 969, 1083, 1305, 1903, 2286, 2370, 3472
PspPPI RGGWCCY 2 cut(s) 2285, 3082
PspXI VCTCGAGB 2 cut(s) 1529, 1702
RsaI GTAC 6 cut(s) 83, 125, 1223, 1695, 3013, 3230
RsaNI GTAC 6 cut(s) 82, 124, 1222, 1694, 3012, 3229
RseI CAYNNNNRTG 3 cut(s) 956, 1992, 3263
SapI GCTCTTC 2 cut(s) 2550, 3488
SaqAI TTAA 8 cut(s) 549, 942, 2613, 2667, 2682, 2772, 2799, 2808
ScaI AGTACT 1 cut(s) 125
SchI GAGTC 5 cut(s) 487, 876, 1057, 1166, 2753
ScrFI CCNGG 8 cut(s) 261, 285, 1033, 1086, 1421, 1577, 2346, 2969
SduI GDGCHC 4 cut(s) 510, 1029, 1042, 2545
SfaNI GCATC 9 cut(s) 791, 1108, 1589, 2228, 2349, 2497, 2611, 2779, 3046
SfcI CTRYAG 1 cut(s) 3126
Sfr274I CTCGAG 3 cut(s) 1529, 1702, 2415
SinI GGWCC 7 cut(s) 106, 379, 469, 1282, 2149, 2285, 3082
SlaI CTCGAG 3 cut(s) 1529, 1702, 2415
SmiMI CAYNNNNRTG 3 cut(s) 956, 1992, 3263
SmlI CTYRAG 9 cut(s) 732, 864, 1174, 1529, 1702, 2415, 2589, 2643, 2700
SmoI CTYRAG 9 cut(s) 732, 864, 1174, 1529, 1702, 2415, 2589, 2643, 2700
SphI GCATGC 1 cut(s) 2930
SseBI AGGCCT 1 cut(s) 1180
SspMI CTAG 9 cut(s) 497, 627, 1490, 2259, 2780, 3030, 3086, 3179, 3296
StuI AGGCCT 1 cut(s) 1180
StyD4I CCNGG 8 cut(s) 259, 283, 1031, 1084, 1419, 1575, 2344, 2967
StyI CCWWGG 5 cut(s) 718, 979, 1858, 1912, 3066
TaiI ACGT 3 cut(s) 79, 138, 425
TaqII GACCGA 2 cut(s) 24, 87
TatI WGTACW 5 cut(s) 123, 1221, 1693, 3011, 3228
TauI GCSGC 5 cut(s) 189, 192, 567, 690, 813
TfiI GAWTC 3 cut(s) 206, 256, 1211
Tru1I TTAA 8 cut(s) 549, 942, 2613, 2667, 2682, 2772, 2799, 2808
Tru9I TTAA 8 cut(s) 549, 942, 2613, 2667, 2682, 2772, 2799, 2808
TscAI CASTG 8 cut(s) 216, 233, 1811, 1881, 1897, 2132, 2356, 2464
TseFI GTSAC 8 cut(s) 20, 617, 1109, 1864, 1918, 1957, 2035, 2104
Tsp45I GTSAC 8 cut(s) 20, 617, 1109, 1864, 1918, 1957, 2035, 2104
TspGWI ACGGA 4 cut(s) 77, 646, 1993, 2576
TspRI CASTG 8 cut(s) 216, 233, 1811, 1881, 1897, 2132, 2356, 2464
Van91I CCANNNNNTGG 1 cut(s) 2948
VneI GTGCAC 1 cut(s) 1025
VpaK11BI GGWCC 7 cut(s) 106, 379, 469, 1282, 2149, 2285, 3082
XagI CCTNNNNNAGG 1 cut(s) 2920
XapI RAATTY 4 cut(s) 2182, 2203, 2487, 3046
XbaI TCTAGA 2 cut(s) 496, 3178
XceI RCATGY 3 cut(s) 1723, 2270, 2930
XcmI CCANNNNNNNNNTGG 1 cut(s) 1591
XhoI CTCGAG 3 cut(s) 1529, 1702, 2415
XmiI GTMKAC 1 cut(s) 765
XmnI GAANNNNTTC 3 cut(s) 1770, 3309, 3482
XspI CTAG 9 cut(s) 497, 627, 1490, 2259, 2780, 3030, 3086, 3179, 3296
ZraI GACGTC 1 cut(s) 136
ZrmI AGTACT 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.