Rh5DG385000

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0014702
Physical Location & Seq
Forward (+)
59262134 .. 59268135
6002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG385000.1

Sequence Viewer

Length: 3522 bp
ATGAAGAAGCGGAGGTATTGTGACTTTGCGGGTCGGTCTTCGACTAACGACGGTGAGACGACGGAAGGAGACCGACGTTCGTACTCGGGTCGGGTTTCGGGTTCGGACCAAACCGAAAGAGAGTACTTTGAAGACGACGGCGGTACGGCTAGGCAACCGTTGAAACCGTCGCAGACGCATTTGAGCGGCGGCTTGCGTCAATGCGAATCACTGTCGATGAAGTTTCAGTGGAACAATCTGCTGGCCCAGAATCCGAATCCGGGTTCTAACCCGAGAAAGGTTCCGGGTCTGAACCGACCCGACTTGGATTATAGCAACCGGAGTGGTAGTTATAGACTGGATTATGAAATGGGTTATGGCGGAAGGGGTTTGGGTGTGGACCAGAGACAGCAGTATGGGCATTTTGATCATCGTGGGTTCACGTCGCCGTTGCCGAAATCCCATCTTGGTGGTCTCCAAGAAAACGAGGAGGACCACCGCCCAATCCCATATGCGACTCTGGAAGACGAGCCCAACTTTAGTTCTGGAACTGGGTATTCATCAATGGAGGGTTTTCGCGGCGTTCGAGATGAATATGACAGAAGCAGAGCAATGGCTGGGTTGCCAATAGGTCACCCGCCTAGACAGAATGAGTTGGTGGACTATGGAGGTTCATCAATTGCTTTGAGTGAGGGTTTTCGCGGGGAGTTCATTAGAAGCAGAGAAATGGGCCATGGTGGATATTCTCAAGGTCAAACAGCGAGAGACGATGAGTTGGTGGACAATGCAGGTGGTGCATCAGAAGCTTTGAATGAGGGTTTTCGCGGCAGAAGCAGAGCAATGGCTGAGGGGCCTGTTGGCAGCCATGCTGGATATACTCAAGGACTTACGCCAAGACACTCACATGGTGAACTCCCATTTGCTTTGAATGAGGGTTTTCGCGGTTCGGATGAGTTTAACAAAAGCAGAGCAATGGCTGAGGGGCCTATTGGCCATGGTGGATATTCTCATGGTCAAACACCAAGAAAGAATGAGTTGGTGCACCCCAGGTGGGCTCAGCAGAGTCCGAAAGAGGAAATGGGTAGGGACTACAAGGTTCCTGGAATGAAAAGAAATGACAATGGTGACATGGATGCCTTGTTCCTTCTTGACTTGCTCTATAAAAAGATGGCTACTGTTGGAGAAGACTCAAGGCCTCCAAGTCATTTGACGCCAAGCGTTGTTGATTCCGTTGTGCACAGGGTTGGTGGTAGCAATGGCTCTGTTGGAGCAGGCACTCATGAGAATTGCCGGTTGGACCAATATCATGATTATATGGAACCAACAAATGTTTTCCCCAACCATCAATCTACAAGTGAACCATCAATCCCCTGCTATGATGAAGTTTTGGGTTCTTCTAACTCAAGATTAGGAGTAGATGTTGGTCATGAAAGAGAGGTCGAGCCTTTATCATATGAAGGTAACCGAGAGAGACTGGTTCTGCCTGATGAAGATCAAGGCTTGCTCGAGGCTTATGGTGGTTGCCAACAGAGGTTGGCAGCAGCCGAAAGCCTGGCATCCATGACCCCTCTGGGAAGGTGGGCATTAAGAAAAGTTGCCATGAAGGAGCAACAATCCGGCAATCACTCAAAGTCTAAGTTCTTCCCTGATTTGAACCCCTCTTGTAATGTACAGAACTCGAGCTATGAAGTTGACATGCAGTTGACTACTACTGAGGATATGCAGCAGCAATATGCTAACGAAGATATTCAACAATATGCTGCTACCTGTGGAGAGGATCATTGGAACATGGAGCAAAATGTGATCTGCGGAGATGGTCAATGGAACATTGACCAAGGTGTGACCCGTGCAGTGGATCAAAGGAACACTGAACAAGGTTCCATTGACCAAGGTGTGACCTGTGGAGTGGATCAAAGGAACATTGCACAAGTTGTGACCTCTGGAGAGGATCAATGTAACATCCATCGAGGTGTGATCTCCAGAGGGAATCAATGGAACAATGATCAAGGTTTGACAACCTATGGAGAGCAACAATGGCGTATGGACAATGGAGGGGAGCAATGGAACAATGATCAAGGCGTCACAACCTCTGGAGAGCAACAGTGGCGTATGAACTATGGAGAGGACCAATGGAATGCTGAGCATATCAACAACCTGAATTCTGACAGTTTGTTACCGAATTCTGAACTGCCAGCATCTACGCAAGAGCATTATGGCGAACAATCCAAATCTGCTAGGATACATGTAAAGGAGCGTTTGGGACCTCCCAAATTATCAACTGGAAGCATAAAAACGCGTTTGAGACCACCTATGAAATCACCTGGCAGTGTGAAGATGCGTTTGGGGCCTTCTGCAAATGATCCTAATATCAACATTTTAGATCCTAATATCAACATTTTAGGTTTCAAGCCTCGAGAACAATACAAATCTCAAATAAGAAAAACAGATGACCTCAGTGGAAGTATTCATCCTGAAGGGGATGGAATTCCAAAAGCTAAGACTGATGCTGCCGCTGGGAGCATTCATCCTCAGGCGAGTGCTCTTCCACAAGTCAAGACGGATGGTTTTATTGGAAGCATTCATCCTCAAGGGGGTGCTCATTCAATAGTTAAGACAGATGCTACTGGAAGCATTCATCCTCAAGGGAATGGTATTCCAAATGTTAAGAAAGGTGATTTTAATGGAAGCATTCGTCCTCAAGGGGCTGGTCTTTCAAAAGTTGATGTGAGAGCTGCAAAGAAAGAACCGCCTGAAGACTCTAAGGAGTTTAAGCAGCTGGTAGATGATGCCTTCATTAAGTTCATTAACATTTTGATTGAGAACCCACCTCAAAGAAGGAAAATCATGGAAGGAAAAGGAGGAAAGTGTTGCGTGTGTGCCAGCAATTCCAAAGAGTTTGTAGAAACGGCAACCTTAGCAAGGCATGCCTTCTTATCGCCAAAGGTTGGTTTCAAAGCAGAACACCTGGGCTTTCATAAAGCACTTTGTGTGCTCTTGGGATGGGACAATACAGCAGTCTCAAACACTAGGTGGGTTCAGCGGAACTTGCCAGATGCAGAATCCTTGGCTCTGAAGGAGGACCTAGTTATCTGGCCTCCTGTTGTTGTCATACACAATAGTTCTGTAGCAGATAAAAATCCTGAAGATGAAAGGCTGACGGTAAATGTTGAAGTTCTAGAGGCTATTCTTACAAGTATAGGATTTGTTGGGAGGAAGACCAATATGTACTGTGGGAAACCTGCCGATGGGAGTGTCATGGTGGTGAAGTTTCAGCCTACATTTTCTGGTTTGCAAGAAGCAGAAAGGCTTCACAAGCTGTTTGCTGATAACAACCATGGGAAGGCTGAGTGGCGGTATATCAATTCTGTTGGCCTCAGCAACAGCAGCAATGGAAAACAAGACTTAGAGGCTGATGAGGCGAATTGTCTATATGGTTATTTGGGAAATGCAGACGATTGGGATGAAGTTGACTTGGGAACCAAGAAGCACTTCGGGGTGAAGAGCAAGAAACAAATCAAGGCTATTGCAAATGCTTCTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

1173

Amino Acids

129.19

Weight (kDa)

5.83

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 1019 - 1147 4.2e-19 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014702)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g29670
malus_domestica MD03G1136700.v1.1
prunus_persica Prupe.6G121100_v2.0.a1 Prupe.6G121100_v2.0.a1
pyrus_communis pycom03g09250
rosa_chinensis RchiOBHm_Chr5g0055031
rosa_laevigata RLG00000034958
rosa_multiflora Rmu_co8511567.1_g000001 Rmu_sc0000925.1_g000013
rosa_roxburghii Rroxscaffold_1G00025460
rosa_rugosa Rorug05G0290200 Rorug05G0290300
rosa_samantha Rh5AG360200 Rh5BG371200 Rh5CG393500 Rh5DG385000
rosa_wichuraiana Rw5G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 758
Acc36I ACCTGC 2 cut(s) 758, 3226
AccB7I CCANNNNNTGG 1 cut(s) 2924
AccBSI CCGCTC 1 cut(s) 186
AccII CGCG 5 cut(s) 558, 681, 804, 921, 2275
AclWI GGATC 6 cut(s) 1764, 1842, 1896, 1935, 2333, 2354
AcoI YGGCCR 1 cut(s) 970
AcsI RAATTY 3 cut(s) 2137, 2158, 2463
AcuI CTGAAG 4 cut(s) 2472, 2751, 3071, 3141
AcyI GRCGYC 2 cut(s) 1190, 2058
AdeI CACNNNGTG 3 cut(s) 887, 2966, 3009
AfaI GTAC 5 cut(s) 83, 125, 145, 1650, 3206
AfiI CCNNNNNNNGG 9 cut(s) 260, 277, 1030, 1831, 2570, 2898, 2924, 3224, 3319
AflIII ACRYGT 2 cut(s) 2221, 2273
AjiI CACGTC 1 cut(s) 423
AjnI CCWGG 5 cut(s) 1025, 1078, 1530, 2299, 2943
AjuI GAANNNNNNNTTGG 6 cut(s) 1256, 1288, 2303, 2335, 3452, 3484
AloI GAACNNNNNNTCC 4 cut(s) 1103, 1135, 1329, 1361
AluBI AGCT 6 cut(s) 785, 1662, 2474, 2711, 2755, 3295
AluI AGCT 6 cut(s) 785, 1662, 2474, 2711, 2755, 3295
Alw21I GWGCWC 5 cut(s) 1023, 1218, 2521, 2578, 2973
Alw26I GTCTC 8 cut(s) 50, 63, 379, 458, 738, 1444, 2275, 3001
Alw44I GTGCAC 2 cut(s) 1019, 1214
AlwI GGATC 6 cut(s) 1764, 1842, 1896, 1935, 2333, 2354
Ama87I CYCGRG 5 cut(s) 85, 271, 1484, 1657, 2391
AoxI GGCC 9 cut(s) 243, 709, 830, 962, 970, 1172, 2324, 3071, 3349
ApaLI GTGCAC 2 cut(s) 1019, 1214
ApoI RAATTY 3 cut(s) 2137, 2158, 2463
Asp700I GAANNNNTTC 3 cut(s) 1725, 3285, 3467
AsuC2I CCSGG 2 cut(s) 261, 285
AsuHPI GGTGA 8 cut(s) 65, 605, 899, 1115, 2289, 2663, 3253, 3487
AvaI CYCGRG 5 cut(s) 85, 271, 1484, 1657, 2391
AvaII GGWCC 7 cut(s) 106, 379, 472, 1276, 2104, 2240, 3058
AxyI CCTNAGG 1 cut(s) 2508
BaeGI GKGCMC 2 cut(s) 1023, 1218
BalI TGGCCA 1 cut(s) 972
BanII GRGCYC 2 cut(s) 513, 1036
BbsI GAAGAC 6 cut(s) 30, 138, 510, 1170, 2739, 3200
Bbv12I GWGCWC 5 cut(s) 1023, 1218, 2521, 2578, 2973
BbvCI CCTCAGC 3 cut(s) 825, 957, 3353
BceAI ACGGC 4 cut(s) 154, 162, 412, 2901
BciT130I CCWGG 5 cut(s) 1027, 1080, 1532, 2301, 2945
BciVI GTATCC 1 cut(s) 2211
BclI TGATCA 3 cut(s) 406, 1981, 2050
BcnI CCSGG 2 cut(s) 261, 285
BcoDI GTCTC 8 cut(s) 50, 63, 379, 458, 738, 1444, 2275, 3001
BfaI CTAG 6 cut(s) 150, 621, 2214, 3006, 3062, 3155
BfmI CTRYAG 1 cut(s) 3102
BfuAI ACCTGC 2 cut(s) 758, 3226
BfuI GTATCC 1 cut(s) 2211
BlpI GCTNAGC 2 cut(s) 1035, 2118
BmcAI AGTACT 1 cut(s) 125
Bme1390I CCNGG 7 cut(s) 261, 285, 1027, 1080, 1532, 2301, 2945
Bme18I GGWCC 7 cut(s) 106, 379, 472, 1276, 2104, 2240, 3058
BmeT110I CYCGRG 5 cut(s) 85, 271, 1484, 1657, 2391
BmgBI CACGTC 1 cut(s) 423
BmiI GGNNCC 9 cut(s) 282, 831, 963, 1077, 1299, 1858, 2241, 2325, 3457
BmrFI CCNGG 7 cut(s) 261, 285, 1027, 1080, 1532, 2301, 2945
BmrI ACTGGG 1 cut(s) 540
BmsI GCATC 9 cut(s) 785, 1102, 1544, 2183, 2304, 2473, 2587, 2755, 3022
BmuI ACTGGG 1 cut(s) 540
BpiI GAAGAC 6 cut(s) 30, 138, 510, 1170, 2739, 3200
BpmI CTGGAG 3 cut(s) 1941, 1942, 2091
Bpu10I CCTNAGC 4 cut(s) 825, 957, 2893, 3353
Bpu1102I GCTNAGC 2 cut(s) 1035, 2118
BpuEI CTTGAG 7 cut(s) 711, 843, 1153, 1366, 2550, 2604, 2661
BpuMI CCSGG 2 cut(s) 261, 285
BsaBI GATNNNNATC 2 cut(s) 1470, 3114
BsaHI GRCGYC 2 cut(s) 1190, 2058
BsaI GGTCTC 3 cut(s) 63, 458, 2275
BsaJI CCNNGG 8 cut(s) 712, 973, 1025, 1813, 1867, 2944, 3042, 3313
BsaWI WCCGGW 1 cut(s) 318
BsaXI ACNNNNNCTCC 5 cut(s) 34, 313, 343, 1383, 1413
Bsc4I CCNNNNNNNGG 9 cut(s) 260, 277, 1030, 1831, 2570, 2898, 2924, 3224, 3319
Bse118I RCCGGY 1 cut(s) 1269
Bse1I ACTGG 5 cut(s) 342, 535, 1458, 2263, 2608
Bse21I CCTNAGG 1 cut(s) 2508
Bse3DI GCAATG 7 cut(s) 597, 825, 957, 1240, 1899, 2045, 3373
Bse8I GATNNNNATC 2 cut(s) 1470, 3114
BseBI CCWGG 5 cut(s) 1027, 1080, 1532, 2301, 2945
BseDI CCNNGG 8 cut(s) 712, 973, 1025, 1813, 1867, 2944, 3042, 3313
BseJI GATNNNNATC 2 cut(s) 1470, 3114
BseLI CCNNNNNNNGG 9 cut(s) 260, 277, 1030, 1831, 2570, 2898, 2924, 3224, 3319
BseMI GCAATG 7 cut(s) 597, 825, 957, 1240, 1899, 2045, 3373
BseMII CTCAG 9 cut(s) 816, 948, 1049, 1683, 2109, 2446, 2522, 3315, 3367
BseNI ACTGG 5 cut(s) 342, 535, 1458, 2263, 2608
BseRI GAGGAG 1 cut(s) 482
BseSI GKGCMC 2 cut(s) 1023, 1218
BseYI CCCAGC 2 cut(s) 596, 2492
BsgI GTGCAG 1 cut(s) 1848
Bsh1236I CGCG 5 cut(s) 558, 681, 804, 921, 2275
BshFI GGCC 9 cut(s) 245, 711, 832, 964, 972, 1174, 2326, 3073, 3351
BsiHKAI GWGCWC 5 cut(s) 1023, 1218, 2521, 2578, 2973
BsiHKCI CYCGRG 5 cut(s) 85, 271, 1484, 1657, 2391
BsiSI CCGG 5 cut(s) 260, 284, 319, 1270, 1596
BslFI GGGAC 3 cut(s) 1079, 2253, 2996
BslI CCNNNNNNNGG 9 cut(s) 260, 277, 1030, 1831, 2570, 2898, 2924, 3224, 3319
BsmAI GTCTC 8 cut(s) 50, 63, 379, 458, 738, 1444, 2275, 3001
BsmBI CGTCTC 2 cut(s) 50, 738
BsmFI GGGAC 3 cut(s) 1079, 2253, 2996
BsmI GAATGC 5 cut(s) 2119, 2499, 2556, 2610, 2667
BsnI GGCC 9 cut(s) 245, 711, 832, 964, 972, 1174, 2326, 3073, 3351
Bso31I GGTCTC 3 cut(s) 63, 458, 2275
BsoBI CYCGRG 5 cut(s) 85, 271, 1484, 1657, 2391
Bsp1286I GDGCHC 7 cut(s) 513, 1023, 1036, 1218, 2521, 2578, 2973
Bsp1407I TGTACA 1 cut(s) 1648
Bsp1720I GCTNAGC 2 cut(s) 1035, 2118
Bsp19I CCATGG 3 cut(s) 712, 973, 3313
BspANI GGCC 9 cut(s) 245, 711, 832, 964, 972, 1174, 2326, 3073, 3351
BspCNI CTCAG 9 cut(s) 817, 949, 1048, 1684, 2110, 2445, 2521, 3316, 3366
BspFNI CGCG 5 cut(s) 558, 681, 804, 921, 2275
BspHI TCATGA 3 cut(s) 1258, 1285, 1405
BspLI GGNNCC 9 cut(s) 282, 831, 963, 1077, 1299, 1858, 2241, 2325, 3457
BspMI ACCTGC 2 cut(s) 758, 3226
BspPI GGATC 6 cut(s) 1764, 1842, 1896, 1935, 2333, 2354
BspQI GCTCTTC 2 cut(s) 2526, 3473
BspTNI GGTCTC 3 cut(s) 63, 458, 2275
BsrBI CCGCTC 1 cut(s) 186
BsrDI GCAATG 7 cut(s) 597, 825, 957, 1240, 1899, 2045, 3373
BsrFI RCCGGY 1 cut(s) 1269
BsrGI TGTACA 1 cut(s) 1648
BsrI ACTGG 5 cut(s) 342, 535, 1458, 2263, 2608
BssAI RCCGGY 1 cut(s) 1269
BssECI CCNNGG 8 cut(s) 712, 973, 1025, 1813, 1867, 2944, 3042, 3313
BssNI GRCGYC 2 cut(s) 1190, 2058
BssT1I CCWWGG 6 cut(s) 712, 973, 1813, 1867, 3042, 3313
Bst2UI CCWGG 5 cut(s) 1027, 1080, 1532, 2301, 2945
Bst4CI ACNGT 9 cut(s) 53, 159, 168, 213, 1156, 2082, 2147, 3139, 3209
Bst6I CTCTTC 2 cut(s) 2526, 3473
BstACI GRCGYC 2 cut(s) 1190, 2058
BstAPI GCANNNNNTGC 2 cut(s) 773, 2903
BstAUI TGTACA 1 cut(s) 1648
BstC8I GCNNGC 7 cut(s) 194, 243, 1252, 1481, 2172, 2860, 2904
BstDSI CCRYGG 3 cut(s) 712, 973, 3313
BstEII GGTNACC 2 cut(s) 611, 1439
BstENI CCTNNNNNAGG 1 cut(s) 2896
BstFNI CGCG 5 cut(s) 558, 681, 804, 921, 2275
BstMAI GTCTC 8 cut(s) 50, 63, 379, 458, 738, 1444, 2275, 3001
BstNI CCWGG 5 cut(s) 1027, 1080, 1532, 2301, 2945
BstNSI RCATGY 3 cut(s) 1678, 2225, 2906
BstPI GGTNACC 2 cut(s) 611, 1439
BstSCI CCNGG 7 cut(s) 259, 283, 1025, 1078, 1530, 2299, 2943
BstSFI CTRYAG 1 cut(s) 3102
BstSLI GKGCMC 2 cut(s) 1023, 1218
BstUI CGCG 5 cut(s) 558, 681, 804, 921, 2275
BstV2I GAAGAC 6 cut(s) 30, 138, 510, 1170, 2739, 3200
BstX2I RGATCY 1 cut(s) 2359
BstXI CCANNNNNNTGG 1 cut(s) 449
BstYI RGATCY 1 cut(s) 2359
Bsu36I CCTNAGG 1 cut(s) 2508
BsuI GTATCC 1 cut(s) 2211
BsuRI GGCC 9 cut(s) 245, 711, 832, 964, 972, 1174, 2326, 3073, 3351
BtgI CCRYGG 3 cut(s) 712, 973, 3313
BtrI CACGTC 1 cut(s) 423
BtsI GCAGTG 2 cut(s) 1836, 2311
BtsIMutI CAGTG 7 cut(s) 209, 233, 1836, 1845, 2087, 2311, 2440
BveI ACCTGC 2 cut(s) 758, 3226
Cac8I GCNNGC 7 cut(s) 194, 243, 1252, 1481, 2172, 2860, 2904
CciI TCATGA 3 cut(s) 1258, 1285, 1405
Cfr10I RCCGGY 1 cut(s) 1269
CseI GACGC 4 cut(s) 184, 185, 1198, 2047
Csp6I GTAC 5 cut(s) 82, 124, 144, 1649, 3205
CspCI CAANNNNNGTGG 4 cut(s) 304, 339, 751, 786
CviQI GTAC 5 cut(s) 82, 124, 144, 1649, 3205
DraIII CACNNNGTG 3 cut(s) 887, 2966, 3009
EaeI YGGCCR 1 cut(s) 970
Eam1104I CTCTTC 2 cut(s) 2526, 3473
EarI CTCTTC 2 cut(s) 2526, 3473
EciI GGCGGA 1 cut(s) 375
Eco130I CCWWGG 6 cut(s) 712, 973, 1813, 1867, 3042, 3313
Eco147I AGGCCT 1 cut(s) 1174
Eco24I GRGCYC 2 cut(s) 513, 1036
Eco31I GGTCTC 3 cut(s) 63, 458, 2275
Eco47I GGWCC 7 cut(s) 106, 379, 472, 1276, 2104, 2240, 3058
Eco57I CTGAAG 4 cut(s) 2472, 2751, 3071, 3141
Eco81I CCTNAGG 1 cut(s) 2508
Eco88I CYCGRG 5 cut(s) 85, 271, 1484, 1657, 2391
Eco91I GGTNACC 2 cut(s) 611, 1439
EcoNI CCTNNNNNAGG 1 cut(s) 2896
EcoO109I RGGNCCY 5 cut(s) 830, 962, 2240, 2324, 3058
EcoO65I GGTNACC 2 cut(s) 611, 1439
EcoRI GAATTC 3 cut(s) 2137, 2158, 2463
EcoRII CCWGG 5 cut(s) 1025, 1078, 1530, 2299, 2943
EcoT14I CCWWGG 6 cut(s) 712, 973, 1813, 1867, 3042, 3313
EcoT38I GRGCYC 2 cut(s) 513, 1036
ErhI CCWWGG 6 cut(s) 712, 973, 1813, 1867, 3042, 3313
Esp3I CGTCTC 2 cut(s) 50, 738
FalI AAGNNNNNCTT 2 cut(s) 3452, 3484
FaqI GGGAC 3 cut(s) 1079, 2253, 2996
FauI CCCGC 3 cut(s) 22, 624, 674
FauNDI CATATG 2 cut(s) 490, 1432
FbaI TGATCA 3 cut(s) 406, 1981, 2050
FriOI GRGCYC 2 cut(s) 513, 1036
FspBI CTAG 6 cut(s) 150, 621, 2214, 3006, 3062, 3155
GsaI CCCAGC 2 cut(s) 600, 2496
GsuI CTGGAG 3 cut(s) 1941, 1942, 2091
HaeIII GGCC 9 cut(s) 245, 711, 832, 964, 972, 1174, 2326, 3073, 3351
HapII CCGG 5 cut(s) 260, 284, 319, 1270, 1596
HgaI GACGC 4 cut(s) 184, 185, 1198, 2047
Hin1I GRCGYC 2 cut(s) 1190, 2058
HincII GTYRAC 3 cut(s) 1672, 1683, 3448
HindII GTYRAC 3 cut(s) 1672, 1683, 3448
HindIII AAGCTT 1 cut(s) 783
HpaII CCGG 5 cut(s) 260, 284, 319, 1270, 1596
HphI GGTGA 8 cut(s) 65, 605, 899, 1115, 2289, 2663, 3253, 3487
Hpy188I TCNGA 9 cut(s) 106, 255, 291, 781, 928, 1047, 2143, 2164, 3051
Hpy99I CGWCG 6 cut(s) 53, 64, 78, 140, 172, 427
HpyCH4III ACNGT 9 cut(s) 53, 159, 168, 213, 1156, 2082, 2147, 3139, 3209
HpyCH4IV ACGT 2 cut(s) 76, 422
HpySE526I ACGT 2 cut(s) 76, 422
Hsp92I GRCGYC 2 cut(s) 1190, 2058
Ksp22I TGATCA 3 cut(s) 406, 1981, 2050
LguI GCTCTTC 2 cut(s) 2526, 3473
LmnI GCTCC 6 cut(s) 1247, 1585, 1771, 2035, 2230, 2496
LweI GCATC 9 cut(s) 785, 1102, 1544, 2183, 2304, 2473, 2587, 2755, 3022
MaeI CTAG 6 cut(s) 150, 621, 2214, 3006, 3062, 3155
MaeII ACGT 2 cut(s) 76, 422
MbiI CCGCTC 1 cut(s) 186
MfeI CAATTG 1 cut(s) 657
MflI RGATCY 1 cut(s) 2359
MhlI GDGCHC 7 cut(s) 513, 1023, 1036, 1218, 2521, 2578, 2973
MlsI TGGCCA 1 cut(s) 972
MluCI AATT 9 cut(s) 657, 1264, 2137, 2158, 2249, 2463, 2863, 3340, 3400
MluI ACGCGT 1 cut(s) 2273
MluNI TGGCCA 1 cut(s) 972
MlyI GAGTC 4 cut(s) 490, 1051, 1160, 2729
MmeI TCCRAC 3 cut(s) 1138, 1225, 1254
Mox20I TGGCCA 1 cut(s) 972
MroXI GAANNNNTTC 3 cut(s) 1725, 3285, 3467
MscI TGGCCA 1 cut(s) 972
MseI TTAA 8 cut(s) 936, 1565, 2589, 2643, 2658, 2748, 2775, 2784
MslI CAYNNNNRTG 4 cut(s) 447, 882, 1947, 3239
Msp20I TGGCCA 1 cut(s) 972
MspA1I CMGCKG 3 cut(s) 2492, 2755, 3019
MspI CCGG 5 cut(s) 260, 284, 319, 1270, 1596
MspR9I CCNGG 7 cut(s) 261, 285, 1027, 1080, 1532, 2301, 2945
MunI CAATTG 1 cut(s) 657
Mva1269I GAATGC 5 cut(s) 2119, 2499, 2556, 2610, 2667
MvaI CCWGG 5 cut(s) 1027, 1080, 1532, 2301, 2945
MvnI CGCG 5 cut(s) 558, 681, 804, 921, 2275
NciI CCSGG 2 cut(s) 261, 285
NcoI CCATGG 3 cut(s) 712, 973, 3313
NdeI CATATG 2 cut(s) 490, 1432
NlaIV GGNNCC 9 cut(s) 282, 831, 963, 1077, 1299, 1858, 2241, 2325, 3457
NmuCI GTSAC 7 cut(s) 20, 611, 1103, 1819, 1873, 1912, 2059
NspI RCATGY 3 cut(s) 1678, 2225, 2906
PaeI GCATGC 1 cut(s) 2906
PaeR7I CTCGAG 3 cut(s) 1484, 1657, 2391
PagI TCATGA 3 cut(s) 1258, 1285, 1405
PaqCI CACCTGC 1 cut(s) 758
PceI AGGCCT 1 cut(s) 1174
PciI ACATGT 1 cut(s) 2221
PciSI GCTCTTC 2 cut(s) 2526, 3473
PcsI WCGNNNNNNNCGW 2 cut(s) 431, 562
PctI GAATGC 5 cut(s) 2119, 2499, 2556, 2610, 2667
PdmI GAANNNNTTC 3 cut(s) 1725, 3285, 3467
PfeI GAWTC 6 cut(s) 206, 250, 256, 1205, 1966, 3038
PflMI CCANNNNNTGG 1 cut(s) 2924
PfoI TCCNGGA 1 cut(s) 1078
PleI GAGTC 4 cut(s) 490, 1050, 1160, 2729
PpsI GAGTC 4 cut(s) 490, 1050, 1160, 2729
PpuMI RGGWCCY 2 cut(s) 2240, 3058
PscI ACATGT 1 cut(s) 2221
Psp5II RGGWCCY 2 cut(s) 2240, 3058
Psp6I CCWGG 5 cut(s) 1025, 1078, 1530, 2299, 2943
PspEI GGTNACC 2 cut(s) 611, 1439
PspFI CCCAGC 2 cut(s) 596, 2492
PspGI CCWGG 5 cut(s) 1025, 1078, 1530, 2299, 2943
PspN4I GGNNCC 9 cut(s) 282, 831, 963, 1077, 1299, 1858, 2241, 2325, 3457
PspPPI RGGWCCY 2 cut(s) 2240, 3058
PspXI VCTCGAGB 2 cut(s) 1484, 1657
PsuI RGATCY 1 cut(s) 2359
PvuII CAGCTG 1 cut(s) 2755
RsaI GTAC 5 cut(s) 83, 125, 145, 1650, 3206
RsaNI GTAC 5 cut(s) 82, 124, 144, 1649, 3205
RseI CAYNNNNRTG 4 cut(s) 447, 882, 1947, 3239
SapI GCTCTTC 2 cut(s) 2526, 3473
SaqAI TTAA 8 cut(s) 936, 1565, 2589, 2643, 2658, 2748, 2775, 2784
ScaI AGTACT 1 cut(s) 125
SchI GAGTC 4 cut(s) 490, 1051, 1160, 2729
ScrFI CCNGG 7 cut(s) 261, 285, 1027, 1080, 1532, 2301, 2945
SduI GDGCHC 7 cut(s) 513, 1023, 1036, 1218, 2521, 2578, 2973
SfaNI GCATC 9 cut(s) 785, 1102, 1544, 2183, 2304, 2473, 2587, 2755, 3022
SfcI CTRYAG 1 cut(s) 3102
Sfr274I CTCGAG 3 cut(s) 1484, 1657, 2391
SinI GGWCC 7 cut(s) 106, 379, 472, 1276, 2104, 2240, 3058
SlaI CTCGAG 3 cut(s) 1484, 1657, 2391
SmiMI CAYNNNNRTG 4 cut(s) 447, 882, 1947, 3239
SphI GCATGC 1 cut(s) 2906
Sse9I AATT 9 cut(s) 657, 1264, 2137, 2158, 2249, 2463, 2863, 3340, 3400
SseBI AGGCCT 1 cut(s) 1174
SspMI CTAG 6 cut(s) 150, 621, 2214, 3006, 3062, 3155
StuI AGGCCT 1 cut(s) 1174
StyD4I CCNGG 7 cut(s) 259, 283, 1025, 1078, 1530, 2299, 2943
StyI CCWWGG 6 cut(s) 712, 973, 1813, 1867, 3042, 3313
TaaI ACNGT 9 cut(s) 53, 159, 168, 213, 1156, 2082, 2147, 3139, 3209
TaiI ACGT 2 cut(s) 79, 425
TaqI TCGA 8 cut(s) 41, 215, 565, 1419, 1485, 1658, 1945, 2392
TaqII GACCGA 2 cut(s) 24, 87
TasI AATT 9 cut(s) 657, 1264, 2137, 2158, 2249, 2463, 2863, 3340, 3400
TatI WGTACW 3 cut(s) 123, 1648, 3204
TauI GCSGC 5 cut(s) 189, 192, 561, 807, 2492
TfiI GAWTC 6 cut(s) 206, 250, 256, 1205, 1966, 3038
Tru1I TTAA 8 cut(s) 936, 1565, 2589, 2643, 2658, 2748, 2775, 2784
Tru9I TTAA 8 cut(s) 936, 1565, 2589, 2643, 2658, 2748, 2775, 2784
TscAI CASTG 7 cut(s) 216, 233, 1836, 1852, 2087, 2311, 2440
TseFI GTSAC 7 cut(s) 20, 611, 1103, 1819, 1873, 1912, 2059
Tsp45I GTSAC 7 cut(s) 20, 611, 1103, 1819, 1873, 1912, 2059
TspGWI ACGGA 3 cut(s) 77, 1198, 2552
TspRI CASTG 7 cut(s) 216, 233, 1836, 1852, 2087, 2311, 2440
Van91I CCANNNNNTGG 1 cut(s) 2924
VneI GTGCAC 2 cut(s) 1019, 1214
VpaK11BI GGWCC 7 cut(s) 106, 379, 472, 1276, 2104, 2240, 3058
XagI CCTNNNNNAGG 1 cut(s) 2896
XapI RAATTY 3 cut(s) 2137, 2158, 2463
XbaI TCTAGA 1 cut(s) 3154
XceI RCATGY 3 cut(s) 1678, 2225, 2906
XcmI CCANNNNNNNNNTGG 1 cut(s) 1546
XhoI CTCGAG 3 cut(s) 1484, 1657, 2391
XmnI GAANNNNTTC 3 cut(s) 1725, 3285, 3467
XspI CTAG 6 cut(s) 150, 621, 2214, 3006, 3062, 3155
ZrmI AGTACT 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.