RchiOBHm_Chr5g0055031

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0014702
Physical Location & Seq
Forward (+)
57807264 .. 57813630
6367 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33208

Sequence Viewer

Length: 3408 bp
ATGAAGAAGCGGAGGTATTGTGACTTTTCGGGTCGGTCTTCGACTAACGACGGTGAGACGACGGAAGGATACCGACGTTCGTACTCGGGTCGGGTTTCGGGTTCGGACCAAACCGAAAGAGAGTACTTTGAAGACGACGTCGGTTCGGATAGGCAACCGTTGAAACCGTCGCAGACACATTTGAGCGGCGGCTTGCGTCAATGCGAATCAATGTCGATGAAGTTTCAGTGGAACAATCTGCTGGCCCAGAATCCGAATCCGGCTTCCAACCCGAGAAAGGTTCCGGGTCTGAACCGACCCGACTTGGATTATAGCAACCGGAGTGGTAGTTATAGACTGGATTATGAAATGGGTTATGGCGGAAGGGTTTTGGGTGTGGACCAGAGAAAGCAGTATGGGCATTTTGATCATCGTGGGTTCACGTCGCCATTGCCGAAATCCCATCTTGGTCTCCAAGAAAACGAGGAGGACCATCGCCCAATCCCATATGCGACTCTGGAAGACGAGCCCAACTTTACTTCTGGAACTGGGTATTCATCAATGGAGGGTTTTCGCGGCGTTCGAGATGAATATGACAGAAGCAGAGCAATGGCTGGGTTGCCAATAGGTCACCCGCCTAGACAGAATGAGTTGGTGGACTATGGAGGTTCATCAATTGCTTTGAGTGAGGGTTTTCGCGGGGAGTTCATTAGAAGCAGAGAAATGGGCCATGGTGGATATTCTCTAGTTCAAACAGCGAGACACGATGAGTTGGTAGACAATGCTGGTGGTGCATCAGAAGCTTTGAATGAGGGTTTTCGTGGCAGAAGCAGAGCAATGGCTGAGGGGCCTGGGCCTATTGGCCATGGTGGATATTCTCATGGTCAAACACCAAGAAAGAATGAGTTGGTGCACCCCAGGTGGGCTCAGCAGAGTCCGAAAGAGGAAATGGGTAGGGACTACAAGGTTCCTGGAATGAAAAGAAATGACAATGGTGACATGGATGCCTTGTTCCTTCTTGACTTGCTCTATAAAAAGATGGCTACTGTTGGAGAAGACTCAAGGCCTCCAAGTCATTTGACGCCAAGCGTTGTTGATTCTGTTGTGTACAGGGTTGGTGGTAGCAATGGCTCTGTTGGAGCAGGCACTCATGAGAATTGCCAGTTGGACCAATATCATGATTATATGGAACCAACAAATGTTTTCCCCAACCATCAATCTACAAGTGAACCATCAATCCCCTGCTATGATGAAGTTTTGGGTTCTTCTAATTCAAGAACAGAGGTGTGCCGGAACAATGGAACCCAGGGCTTCAGATTAGGAGTAGATGTTGGTCATGAAAGAGAGGTCGAGCCTTTATCATATGAAGGTAACCTAGAGAGACTGGTTCTGCCTGATGAAGATCAAGGCTTGCTCGAGGCTTATGGTGGTTGCCAACAGAGGTTGGCAGCAGCCGAAAGCCTGGCATCCATGACCCCTCTGGGAAGGTGGGCATTGAGGAAAGTTGCCATGAAGGAGCAACAATCCGGCAATCACTCAAAGTCTAAGTTCTTCCCTGATTTGAACCCCTCTTGTAATGTACAGAACTCGAGCTATGAAGTTGACATGCAGTTGACTACTACTGAGGATATGCAGCAGCAATATGCTAACGAAGATATTCAACAATATGCTGCTACCTGTGGAGAGGATCATTGGAACATGGAGCAAAATGTGATCTGTGGAGATGGTCAATGGAACATTGACCAAGGTGTGACCCGTGCAGTGGATCAAAGGAACACTGAACAAGGTTCCATTGACCAAGGTGTGACCTGTGGGGTGGATCAAAGGAACATTGCACAAGGTGTGACCTCTGGAGAGGATCAATGTAACATCCATCGAGGTGTGATCTCCGTAGGGGATCAATGGAACAATGATCAAGGTTTGACAACCTATGGAGAGCAACAATGGTGTATGGACAATGGAGGGGAGCAATGGAACAATGATCAAGGCGTCACAACCTCAGGAGAGCAACAGTGGCGTATGAACTATGGAGAGGACCAATGGAATGCTGAGCATATCAACAACCTGAATTCTGACAGTTTGTTACCAAATTCTGAACTGCCAGCATCTACGCAAGAGCATTATGGCGAACAATCCAAATCTGCTAGGATACATGTAAAGGAGCGTTTGGGACCTCCCAAATTATCAACTGGAAACATAAAAACGCGTTCGAGACCACCTATGAAATCACCTGGCAGTGTGAAGATGCGTTTGGGGCCTTCTGCAAATGATCCTAATATCAACATTTTAGGTTTCAAGCCTCGAGAACAATACAAATCTCGAATAAGAAAAACAGATGACTTCAGTGGAAGTATTCATCCTGAAGGGGATGGAATTCCAAAAGCTAAGACTGATGCTGCCGCTGGGAGCATTCATCCTCAGGGGAGTGCTCTTCCACAAGTCAAGACGGATGGTTTTATTGGAAGCATTCATCCTCAAGGGGGTGGTCATTCAATAGTTAAGACAGATGCTACTGGAAGCATTTATCCTCAAGGGAATGGTATTCCAAATGTTAAGGAAGGTGATTTTAATGGAAGCATTCGTCCTCAAGGGGGTAGTCTTTCCAAAGTTGATGTGAGAGCTGCAAAGAAAGAACCGCCTGAAGACTCTAAGGAGTTTAAGCAGCTGGTAGATGATGCCTTCATTAAGTTCATTAACATTTTGAAAGAGAACCCACCTCAAAGAAGGAAAATCATGGAAGGAAAAGGAGGAAAGTGTTGCGTGTGTGCCAGCAATTCCAAAGAGTTTGTAGAAACGGCAACCTTAGCAAGGCATGCCTTCTTGTCGCCAAAGGTTGGTTTCAAAGCAGAACACCTGGGCTTTCATAAAGCACTTTGTGTGCTCTTGGGATGGGACAGTACAGCAGTTTCAAACACTAGGTGGGTTCAGCAGAATTTGCCAGATGCAGAATCCTTGGCTCTGAAGGAGGACCTAGTTATCTGGCCTCCTGTTGTTGTCATACACAATAGTTCTGTCGCAGATAAAAATCCTGAAGATGAAAGGCTGACGGTAAATGTTGAAGTTCTAGAGGCTATTCTTACAAGTATAGGATTTGTTGGGAAGAAGACCATGTACTGTGGGAAACCTGCCGATGGGAGTGTCATGGTGGTGAAGTTTCAGCCTACATTTTCTGGTTTGCAAGAAGCAGAAAGGCTTCACAAGCTGTTTGCTGATAACAAACATGGGAAGGCTGAGTGGCGGTACATCAATTCTGTTGGCCTCAGCAACAGCAGCAATGGAAAGCAAGACTTAGAGGCTGATGAGGCGAATTGTCTATATGGTTATTTGGGAAATGCAGACGATTGGGACGAACTTGACTTGGGAACCAAGAAGCACTTCGGGGTGAAGAGCAAGAAACAAATCAAGGCTATTGCAAATGCTTCTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

1135

Amino Acids

125.28

Weight (kDa)

5.75

Isoelectric Point (pI)

39.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 982 - 1109 5.3e-18 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014702)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g29670
malus_domestica MD03G1136700.v1.1
prunus_persica Prupe.6G121100_v2.0.a1 Prupe.6G121100_v2.0.a1
pyrus_communis pycom03g09250
rosa_chinensis RchiOBHm_Chr5g0055031
rosa_laevigata RLG00000034958
rosa_multiflora Rmu_co8511567.1_g000001 Rmu_sc0000925.1_g000013
rosa_roxburghii Rroxscaffold_1G00025460
rosa_rugosa Rorug05G0290200 Rorug05G0290300
rosa_samantha Rh5AG360200 Rh5BG371200 Rh5CG393500 Rh5DG385000
rosa_wichuraiana Rw5G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 141
Acc36I ACCTGC 1 cut(s) 3112
AccB7I CCANNNNNTGG 1 cut(s) 2813
AccBSI CCGCTC 1 cut(s) 186
AccI GTMKAC 1 cut(s) 756
AccII CGCG 3 cut(s) 555, 678, 2185
AclWI GGATC 6 cut(s) 1674, 1752, 1806, 1845, 1884, 2243
AcoI YGGCCR 1 cut(s) 841
AcsI RAATTY 4 cut(s) 2047, 2068, 2352, 2911
AcuI CTGAAG 6 cut(s) 1276, 2305, 2361, 2640, 2960, 3030
AcyI GRCGYC 3 cut(s) 138, 1061, 1968
AdeI CACNNNGTG 3 cut(s) 1820, 2855, 2898
AfaI GTAC 7 cut(s) 83, 125, 1088, 1560, 2878, 3092, 3221
AfiI CCNNNNNNNGG 8 cut(s) 277, 901, 1741, 2459, 2570, 2787, 2813, 3110
AflIII ACRYGT 2 cut(s) 2131, 2183
AjiI CACGTC 1 cut(s) 423
AjnI CCWGG 7 cut(s) 829, 896, 949, 1284, 1440, 2209, 2832
AjuI GAANNNNNNNTTGG 6 cut(s) 1127, 1159, 2213, 2245, 3338, 3370
AloI GAACNNNNNNTCC 4 cut(s) 974, 1006, 1200, 1232
AluBI AGCT 6 cut(s) 782, 1572, 2363, 2600, 2644, 3181
AluI AGCT 6 cut(s) 782, 1572, 2363, 2600, 2644, 3181
Alw21I GWGCWC 3 cut(s) 894, 2410, 2862
Alw26I GTCTC 5 cut(s) 50, 455, 733, 1354, 2185
Alw44I GTGCAC 1 cut(s) 890
AlwI GGATC 6 cut(s) 1674, 1752, 1806, 1845, 1884, 2243
Ama87I CYCGRG 5 cut(s) 85, 271, 1394, 1567, 2280
AoxI GGCC 9 cut(s) 243, 706, 827, 833, 841, 1043, 2234, 2960, 3235
ApaLI GTGCAC 1 cut(s) 890
ApeKI GCWGC 9 cut(s) 1427, 1430, 1612, 1615, 1649, 2375, 2600, 2641, 3249
ApoI RAATTY 4 cut(s) 2047, 2068, 2352, 2911
Asp700I GAANNNNTTC 3 cut(s) 1635, 3171, 3353
AsuC2I CCSGG 1 cut(s) 285
AsuHPI GGTGA 7 cut(s) 65, 602, 986, 2199, 2552, 3139, 3373
AvaI CYCGRG 5 cut(s) 85, 271, 1394, 1567, 2280
AvaII GGWCC 7 cut(s) 106, 379, 469, 1147, 2014, 2150, 2947
AxyI CCTNAGG 2 cut(s) 1978, 2397
BaeGI GKGCMC 1 cut(s) 894
BaeI ACNNNNGTAYC 2 cut(s) 61, 94
BalI TGGCCA 1 cut(s) 843
BanII GRGCYC 2 cut(s) 510, 907
BarI GAAGNNNNNNTAC 2 cut(s) 3074, 3106
BbsI GAAGAC 6 cut(s) 30, 138, 507, 1041, 2628, 3089
Bbv12I GWGCWC 3 cut(s) 894, 2410, 2862
BbvCI CCTCAGC 2 cut(s) 822, 3239
BbvI GCAGC 9 cut(s) 1439, 1442, 1624, 1627, 1636, 2362, 2587, 2653, 3261
BceAI ACGGC 1 cut(s) 2790
BciT130I CCWGG 7 cut(s) 831, 898, 951, 1286, 1442, 2211, 2834
BciVI GTATCC 2 cut(s) 62, 2121
BclI TGATCA 3 cut(s) 406, 1891, 1960
BcnI CCSGG 1 cut(s) 285
BcoDI GTCTC 5 cut(s) 50, 455, 733, 1354, 2185
BfaI CTAG 7 cut(s) 618, 725, 1355, 2124, 2895, 2951, 3044
BfuAI ACCTGC 1 cut(s) 3112
BfuI GTATCC 2 cut(s) 62, 2121
BlpI GCTNAGC 2 cut(s) 906, 2028
BmcAI AGTACT 1 cut(s) 125
Bme1390I CCNGG 8 cut(s) 285, 831, 898, 951, 1286, 1442, 2211, 2834
Bme18I GGWCC 7 cut(s) 106, 379, 469, 1147, 2014, 2150, 2947
BmeT110I CYCGRG 5 cut(s) 85, 271, 1394, 1567, 2280
BmgBI CACGTC 1 cut(s) 423
BmiI GGNNCC 9 cut(s) 282, 828, 948, 1170, 1282, 1768, 2151, 2235, 3343
BmrFI CCNGG 8 cut(s) 285, 831, 898, 951, 1286, 1442, 2211, 2834
BmrI ACTGGG 1 cut(s) 537
BmsI GCATC 9 cut(s) 782, 973, 1454, 2093, 2214, 2362, 2476, 2644, 2911
BmuI ACTGGG 1 cut(s) 537
BpiI GAAGAC 6 cut(s) 30, 138, 507, 1041, 2628, 3089
BpmI CTGGAG 1 cut(s) 1851
Bpu10I CCTNAGC 3 cut(s) 822, 2782, 3239
Bpu1102I GCTNAGC 2 cut(s) 906, 2028
BpuEI CTTGAG 4 cut(s) 1024, 2439, 2493, 2550
BpuMI CCSGG 1 cut(s) 285
BsaBI GATNNNNATC 2 cut(s) 1380, 3003
BsaHI GRCGYC 3 cut(s) 138, 1061, 1968
BsaI GGTCTC 2 cut(s) 455, 2185
BsaWI WCCGGW 1 cut(s) 318
BsaXI ACNNNNNCTCC 5 cut(s) 34, 313, 343, 1293, 1323
Bsc4I CCNNNNNNNGG 8 cut(s) 277, 901, 1741, 2459, 2570, 2787, 2813, 3110
Bse1I ACTGG 6 cut(s) 342, 532, 1141, 1368, 2173, 2497
Bse21I CCTNAGG 2 cut(s) 1978, 2397
Bse3DI GCAATG 7 cut(s) 428, 594, 822, 1111, 1809, 1955, 3259
Bse8I GATNNNNATC 2 cut(s) 1380, 3003
BseBI CCWGG 7 cut(s) 831, 898, 951, 1286, 1442, 2211, 2834
BseGI GGATG 9 cut(s) 988, 1445, 1848, 2335, 2353, 2392, 2434, 2449, 2873
BseJI GATNNNNATC 2 cut(s) 1380, 3003
BseLI CCNNNNNNNGG 8 cut(s) 277, 901, 1741, 2459, 2570, 2787, 2813, 3110
BseMI GCAATG 7 cut(s) 428, 594, 822, 1111, 1809, 1955, 3259
BseMII CTCAG 8 cut(s) 813, 920, 1593, 1992, 2019, 2411, 3201, 3253
BseNI ACTGG 6 cut(s) 342, 532, 1141, 1368, 2173, 2497
BseRI GAGGAG 1 cut(s) 479
BseSI GKGCMC 1 cut(s) 894
BseXI GCAGC 9 cut(s) 1439, 1442, 1624, 1627, 1636, 2362, 2587, 2653, 3261
BseYI CCCAGC 2 cut(s) 593, 2381
BsgI GTGCAG 1 cut(s) 1758
Bsh1236I CGCG 3 cut(s) 555, 678, 2185
BshFI GGCC 9 cut(s) 245, 708, 829, 835, 843, 1045, 2236, 2962, 3237
BsiHKAI GWGCWC 3 cut(s) 894, 2410, 2862
BsiHKCI CYCGRG 5 cut(s) 85, 271, 1394, 1567, 2280
BsiSI CCGG 5 cut(s) 260, 284, 319, 1270, 1506
BslFI GGGAC 4 cut(s) 950, 2163, 2885, 3338
BslI CCNNNNNNNGG 8 cut(s) 277, 901, 1741, 2459, 2570, 2787, 2813, 3110
BsmAI GTCTC 5 cut(s) 50, 455, 733, 1354, 2185
BsmBI CGTCTC 1 cut(s) 50
BsmFI GGGAC 4 cut(s) 950, 2163, 2885, 3338
BsmI GAATGC 4 cut(s) 2029, 2388, 2445, 2556
BsnI GGCC 9 cut(s) 245, 708, 829, 835, 843, 1045, 2236, 2962, 3237
Bso31I GGTCTC 2 cut(s) 455, 2185
BsoBI CYCGRG 5 cut(s) 85, 271, 1394, 1567, 2280
Bsp1286I GDGCHC 5 cut(s) 510, 894, 907, 2410, 2862
Bsp1407I TGTACA 2 cut(s) 1086, 1558
Bsp1720I GCTNAGC 2 cut(s) 906, 2028
Bsp19I CCATGG 2 cut(s) 709, 844
BspANI GGCC 9 cut(s) 245, 708, 829, 835, 843, 1045, 2236, 2962, 3237
BspCNI CTCAG 8 cut(s) 814, 919, 1594, 1991, 2020, 2410, 3202, 3252
BspFNI CGCG 3 cut(s) 555, 678, 2185
BspHI TCATGA 3 cut(s) 1129, 1156, 1315
BspLI GGNNCC 9 cut(s) 282, 828, 948, 1170, 1282, 1768, 2151, 2235, 3343
BspMI ACCTGC 1 cut(s) 3112
BspPI GGATC 6 cut(s) 1674, 1752, 1806, 1845, 1884, 2243
BspQI GCTCTTC 2 cut(s) 2415, 3359
BspTNI GGTCTC 2 cut(s) 455, 2185
BsrBI CCGCTC 1 cut(s) 186
BsrDI GCAATG 7 cut(s) 428, 594, 822, 1111, 1809, 1955, 3259
BsrGI TGTACA 2 cut(s) 1086, 1558
BsrI ACTGG 6 cut(s) 342, 532, 1141, 1368, 2173, 2497
BssNI GRCGYC 3 cut(s) 138, 1061, 1968
BssT1I CCWWGG 5 cut(s) 709, 844, 1723, 1777, 2931
Bst2UI CCWGG 7 cut(s) 831, 898, 951, 1286, 1442, 2211, 2834
Bst4CI ACNGT 9 cut(s) 53, 159, 168, 1027, 1992, 2057, 2876, 3028, 3095
Bst6I CTCTTC 2 cut(s) 2415, 3359
BstACI GRCGYC 3 cut(s) 138, 1061, 1968
BstAPI GCANNNNNTGC 2 cut(s) 2792, 2914
BstAUI TGTACA 2 cut(s) 1086, 1558
BstC8I GCNNGC 7 cut(s) 194, 243, 1123, 1391, 2082, 2749, 2793
BstDSI CCRYGG 2 cut(s) 709, 844
BstEII GGTNACC 2 cut(s) 608, 1349
BstENI CCTNNNNNAGG 1 cut(s) 2785
BstF5I GGATG 9 cut(s) 988, 1445, 1848, 2335, 2353, 2392, 2434, 2449, 2873
BstFNI CGCG 3 cut(s) 555, 678, 2185
BstMAI GTCTC 5 cut(s) 50, 455, 733, 1354, 2185
BstNI CCWGG 7 cut(s) 831, 898, 951, 1286, 1442, 2211, 2834
BstNSI RCATGY 3 cut(s) 1588, 2135, 2795
BstPI GGTNACC 2 cut(s) 608, 1349
BstSCI CCNGG 8 cut(s) 283, 829, 896, 949, 1284, 1440, 2209, 2832
BstSLI GKGCMC 1 cut(s) 894
BstUI CGCG 3 cut(s) 555, 678, 2185
BstV1I GCAGC 9 cut(s) 1439, 1442, 1624, 1627, 1636, 2362, 2587, 2653, 3261
BstV2I GAAGAC 6 cut(s) 30, 138, 507, 1041, 2628, 3089
Bsu36I CCTNAGG 2 cut(s) 1978, 2397
BsuI GTATCC 2 cut(s) 62, 2121
BsuRI GGCC 9 cut(s) 245, 708, 829, 835, 843, 1045, 2236, 2962, 3237
BtgI CCRYGG 2 cut(s) 709, 844
BtgZI GCGATG 1 cut(s) 458
BtrI CACGTC 1 cut(s) 423
BtsCI GGATG 9 cut(s) 988, 1445, 1848, 2335, 2353, 2392, 2434, 2449, 2873
BtsI GCAGTG 2 cut(s) 1746, 2221
BtsIMutI CAGTG 6 cut(s) 233, 1746, 1755, 1997, 2221, 2329
BveI ACCTGC 1 cut(s) 3112
Cac8I GCNNGC 7 cut(s) 194, 243, 1123, 1391, 2082, 2749, 2793
CciI TCATGA 3 cut(s) 1129, 1156, 1315
CseI GACGC 3 cut(s) 185, 1069, 1957
Csp6I GTAC 7 cut(s) 82, 124, 1087, 1559, 2877, 3091, 3220
CspCI CAANNNNNGTGG 4 cut(s) 304, 339, 748, 783
CviQI GTAC 7 cut(s) 82, 124, 1087, 1559, 2877, 3091, 3220
DraIII CACNNNGTG 3 cut(s) 1820, 2855, 2898
EaeI YGGCCR 1 cut(s) 841
Eam1104I CTCTTC 2 cut(s) 2415, 3359
EarI CTCTTC 2 cut(s) 2415, 3359
EciI GGCGGA 1 cut(s) 375
Eco130I CCWWGG 5 cut(s) 709, 844, 1723, 1777, 2931
Eco147I AGGCCT 1 cut(s) 1045
Eco24I GRGCYC 2 cut(s) 510, 907
Eco31I GGTCTC 2 cut(s) 455, 2185
Eco47I GGWCC 7 cut(s) 106, 379, 469, 1147, 2014, 2150, 2947
Eco57I CTGAAG 6 cut(s) 1276, 2305, 2361, 2640, 2960, 3030
Eco81I CCTNAGG 2 cut(s) 1978, 2397
Eco88I CYCGRG 5 cut(s) 85, 271, 1394, 1567, 2280
Eco91I GGTNACC 2 cut(s) 608, 1349
EcoNI CCTNNNNNAGG 1 cut(s) 2785
EcoO109I RGGNCCY 4 cut(s) 827, 2150, 2234, 2947
EcoO65I GGTNACC 2 cut(s) 608, 1349
EcoRI GAATTC 2 cut(s) 2047, 2352
EcoRII CCWGG 7 cut(s) 829, 896, 949, 1284, 1440, 2209, 2832
EcoT14I CCWWGG 5 cut(s) 709, 844, 1723, 1777, 2931
EcoT38I GRGCYC 2 cut(s) 510, 907
ErhI CCWWGG 5 cut(s) 709, 844, 1723, 1777, 2931
Esp3I CGTCTC 1 cut(s) 50
FalI AAGNNNNNCTT 4 cut(s) 3251, 3283, 3338, 3370
FaqI GGGAC 4 cut(s) 950, 2163, 2885, 3338
FauI CCCGC 2 cut(s) 621, 671
FauNDI CATATG 2 cut(s) 487, 1342
FbaI TGATCA 3 cut(s) 406, 1891, 1960
FblI GTMKAC 1 cut(s) 756
FokI GGATG 9 cut(s) 995, 1432, 1835, 2322, 2360, 2379, 2436, 2441, 2880
FriOI GRGCYC 2 cut(s) 510, 907
FspBI CTAG 7 cut(s) 618, 725, 1355, 2124, 2895, 2951, 3044
GsaI CCCAGC 2 cut(s) 597, 2385
GsuI CTGGAG 1 cut(s) 1851
HaeIII GGCC 9 cut(s) 245, 708, 829, 835, 843, 1045, 2236, 2962, 3237
HapII CCGG 5 cut(s) 260, 284, 319, 1270, 1506
HgaI GACGC 3 cut(s) 185, 1069, 1957
Hin1I GRCGYC 3 cut(s) 138, 1061, 1968
HincII GTYRAC 2 cut(s) 1582, 1593
HindII GTYRAC 2 cut(s) 1582, 1593
HindIII AAGCTT 1 cut(s) 780
HinfI GANTC 9 cut(s) 206, 250, 256, 493, 913, 1037, 1076, 2624, 2927
HpaII CCGG 5 cut(s) 260, 284, 319, 1270, 1506
HphI GGTGA 7 cut(s) 65, 602, 986, 2199, 2552, 3139, 3373
Hpy166II GTNNAC 9 cut(s) 379, 420, 637, 757, 892, 1087, 1208, 1582, 1593
Hpy8I GTNNAC 9 cut(s) 379, 420, 637, 757, 892, 1087, 1208, 1582, 1593
Hpy99I CGWCG 7 cut(s) 53, 64, 78, 140, 143, 172, 427
HpyCH4III ACNGT 9 cut(s) 53, 159, 168, 1027, 1992, 2057, 2876, 3028, 3095
HpyCH4IV ACGT 3 cut(s) 76, 138, 422
HpySE526I ACGT 3 cut(s) 76, 138, 422
Hsp92I GRCGYC 3 cut(s) 138, 1061, 1968
Ksp22I TGATCA 3 cut(s) 406, 1891, 1960
LguI GCTCTTC 2 cut(s) 2415, 3359
LmnI GCTCC 6 cut(s) 1118, 1495, 1681, 1945, 2140, 2385
Lsp1109I GCAGC 9 cut(s) 1439, 1442, 1624, 1627, 1636, 2362, 2587, 2653, 3261
LweI GCATC 9 cut(s) 782, 973, 1454, 2093, 2214, 2362, 2476, 2644, 2911
MaeI CTAG 7 cut(s) 618, 725, 1355, 2124, 2895, 2951, 3044
MaeII ACGT 3 cut(s) 76, 138, 422
MbiI CCGCTC 1 cut(s) 186
MfeI CAATTG 1 cut(s) 654
MhlI GDGCHC 5 cut(s) 510, 894, 907, 2410, 2862
MlsI TGGCCA 1 cut(s) 843
MluI ACGCGT 1 cut(s) 2183
MluNI TGGCCA 1 cut(s) 843
MlyI GAGTC 4 cut(s) 487, 922, 1031, 2618
MmeI TCCRAC 4 cut(s) 291, 1009, 1096, 1125
Mox20I TGGCCA 1 cut(s) 843
MroXI GAANNNNTTC 3 cut(s) 1635, 3171, 3353
MscI TGGCCA 1 cut(s) 843
MseI TTAA 6 cut(s) 2478, 2532, 2547, 2637, 2664, 2673
MslI CAYNNNNRTG 2 cut(s) 1857, 3125
Msp20I TGGCCA 1 cut(s) 843
MspA1I CMGCKG 2 cut(s) 2381, 2644
MspI CCGG 5 cut(s) 260, 284, 319, 1270, 1506
MspR9I CCNGG 8 cut(s) 285, 831, 898, 951, 1286, 1442, 2211, 2834
MunI CAATTG 1 cut(s) 654
Mva1269I GAATGC 4 cut(s) 2029, 2388, 2445, 2556
MvaI CCWGG 7 cut(s) 831, 898, 951, 1286, 1442, 2211, 2834
MvnI CGCG 3 cut(s) 555, 678, 2185
NciI CCSGG 1 cut(s) 285
NcoI CCATGG 2 cut(s) 709, 844
NdeI CATATG 2 cut(s) 487, 1342
NlaIV GGNNCC 9 cut(s) 282, 828, 948, 1170, 1282, 1768, 2151, 2235, 3343
NmuCI GTSAC 7 cut(s) 20, 608, 974, 1729, 1783, 1822, 1969
NspI RCATGY 3 cut(s) 1588, 2135, 2795
PaeI GCATGC 1 cut(s) 2795
PaeR7I CTCGAG 3 cut(s) 1394, 1567, 2280
PagI TCATGA 3 cut(s) 1129, 1156, 1315
PasI CCCWGGG 1 cut(s) 1285
PceI AGGCCT 1 cut(s) 1045
PciI ACATGT 1 cut(s) 2131
PciSI GCTCTTC 2 cut(s) 2415, 3359
PcsI WCGNNNNNNNCGW 3 cut(s) 431, 559, 3324
PctI GAATGC 4 cut(s) 2029, 2388, 2445, 2556
PdmI GAANNNNTTC 3 cut(s) 1635, 3171, 3353
PfeI GAWTC 5 cut(s) 206, 250, 256, 1076, 2927
PflFI GACNNNGTC 1 cut(s) 137
PflMI CCANNNNNTGG 1 cut(s) 2813
PfoI TCCNGGA 1 cut(s) 949
PleI GAGTC 4 cut(s) 487, 921, 1031, 2618
PpsI GAGTC 4 cut(s) 487, 921, 1031, 2618
PpuMI RGGWCCY 2 cut(s) 2150, 2947
PscI ACATGT 1 cut(s) 2131
Psp5II RGGWCCY 2 cut(s) 2150, 2947
Psp6I CCWGG 7 cut(s) 829, 896, 949, 1284, 1440, 2209, 2832
PspEI GGTNACC 2 cut(s) 608, 1349
PspFI CCCAGC 2 cut(s) 593, 2381
PspGI CCWGG 7 cut(s) 829, 896, 949, 1284, 1440, 2209, 2832
PspN4I GGNNCC 9 cut(s) 282, 828, 948, 1170, 1282, 1768, 2151, 2235, 3343
PspPPI RGGWCCY 2 cut(s) 2150, 2947
PspXI VCTCGAGB 2 cut(s) 1394, 1567
PsyI GACNNNGTC 1 cut(s) 137
PvuII CAGCTG 1 cut(s) 2644
RsaI GTAC 7 cut(s) 83, 125, 1088, 1560, 2878, 3092, 3221
RsaNI GTAC 7 cut(s) 82, 124, 1087, 1559, 2877, 3091, 3220
RseI CAYNNNNRTG 2 cut(s) 1857, 3125
SapI GCTCTTC 2 cut(s) 2415, 3359
SaqAI TTAA 6 cut(s) 2478, 2532, 2547, 2637, 2664, 2673
ScaI AGTACT 1 cut(s) 125
SchI GAGTC 4 cut(s) 487, 922, 1031, 2618
ScrFI CCNGG 8 cut(s) 285, 831, 898, 951, 1286, 1442, 2211, 2834
SduI GDGCHC 5 cut(s) 510, 894, 907, 2410, 2862
SfaNI GCATC 9 cut(s) 782, 973, 1454, 2093, 2214, 2362, 2476, 2644, 2911
Sfr274I CTCGAG 3 cut(s) 1394, 1567, 2280
SinI GGWCC 7 cut(s) 106, 379, 469, 1147, 2014, 2150, 2947
SlaI CTCGAG 3 cut(s) 1394, 1567, 2280
SmiMI CAYNNNNRTG 2 cut(s) 1857, 3125
SmlI CTYRAG 7 cut(s) 1039, 1394, 1567, 2280, 2454, 2508, 2565
SmoI CTYRAG 7 cut(s) 1039, 1394, 1567, 2280, 2454, 2508, 2565
SphI GCATGC 1 cut(s) 2795
SseBI AGGCCT 1 cut(s) 1045
SspMI CTAG 7 cut(s) 618, 725, 1355, 2124, 2895, 2951, 3044
StuI AGGCCT 1 cut(s) 1045
StyD4I CCNGG 8 cut(s) 283, 829, 896, 949, 1284, 1440, 2209, 2832
StyI CCWWGG 5 cut(s) 709, 844, 1723, 1777, 2931
TaaI ACNGT 9 cut(s) 53, 159, 168, 1027, 1992, 2057, 2876, 3028, 3095
TaiI ACGT 3 cut(s) 79, 141, 425
TaqII GACCGA 1 cut(s) 24
TatI WGTACW 5 cut(s) 123, 1086, 1558, 2876, 3090
TauI GCSGC 4 cut(s) 189, 192, 558, 2381
TfiI GAWTC 5 cut(s) 206, 250, 256, 1076, 2927
Tru1I TTAA 6 cut(s) 2478, 2532, 2547, 2637, 2664, 2673
Tru9I TTAA 6 cut(s) 2478, 2532, 2547, 2637, 2664, 2673
TscAI CASTG 6 cut(s) 233, 1746, 1762, 1997, 2221, 2329
TseFI GTSAC 7 cut(s) 20, 608, 974, 1729, 1783, 1822, 1969
TseI GCWGC 9 cut(s) 1427, 1430, 1612, 1615, 1649, 2375, 2600, 2641, 3249
Tsp45I GTSAC 7 cut(s) 20, 608, 974, 1729, 1783, 1822, 1969
TspGWI ACGGA 3 cut(s) 77, 1858, 2441
TspRI CASTG 6 cut(s) 233, 1746, 1762, 1997, 2221, 2329
Tth111I GACNNNGTC 1 cut(s) 137
Van91I CCANNNNNTGG 1 cut(s) 2813
VneI GTGCAC 1 cut(s) 890
VpaK11BI GGWCC 7 cut(s) 106, 379, 469, 1147, 2014, 2150, 2947
XagI CCTNNNNNAGG 1 cut(s) 2785
XapI RAATTY 4 cut(s) 2047, 2068, 2352, 2911
XbaI TCTAGA 1 cut(s) 3043
XceI RCATGY 3 cut(s) 1588, 2135, 2795
XcmI CCANNNNNNNNNTGG 1 cut(s) 1456
XhoI CTCGAG 3 cut(s) 1394, 1567, 2280
XmiI GTMKAC 1 cut(s) 756
XmnI GAANNNNTTC 3 cut(s) 1635, 3171, 3353
XspI CTAG 7 cut(s) 618, 725, 1355, 2124, 2895, 2951, 3044
ZraI GACGTC 1 cut(s) 139
ZrmI AGTACT 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.