MD03G1136700.v1.1

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014702
Physical Location & Seq
Forward (+)
13743556 .. 13748440
4885 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1136700.v1.1.491

Sequence Viewer

Length: 2544 bp
ATGAGTGGGCGGAGGCTCGGGGACTACCCGAGTCGTTCTTTGGCGAACCACTGCGCGAAAGCCGAGGAGAGTCGACGTTCGTACTCGGGTCGGGTTTCGGGTTCGGCAAGAGCAAGACCTGAACCGAATTCCCGGAGAGAGTACGATAACCGTCGTTATTATGATGGAGAAAGAGAGAGAGACAGGCGGCCGCAGGAACCGGCAGGTCTCGGCGCCGGCTTGCCCCTCTCGGAATCGACGTCGATGAAGTTCGAGTGGAACAATCTGCTAGCCCGGAGACCCGCCGTTTCGACCGCGAATCCGGGTTGGAACCGACCCGACTTCGGTTTCAGCTCCGGAAGTGGGAAACTTGGGGTTCCGGACCCTGGTGTGGACCCCACAAGGAAGCTTGCGTATTTTCATGGCGGATTCCCGGCTGCGTTGCCAAAACCCCATGTGGGTATTCAAAAAGTTGAAGACTTTAGGCTCGGTGACCACAATGCATACGTGGGTCCAGAAGATAAGGCCGTGTTTGGCAACGGACCTGCGCGCTCGAGCATTTTGGATCCGGGTTTTCACGGGGTTTCTGATGAATTCAACACAAGTCAAATAACGGGACCACATCAGTCGGTTGATGCCGGTTGGGGATGGCGGAATCCGAAATGGGACTACAATGCCCCTGCATATGGTGTTAGAGAAGTTCCGAGGCCTCCGGTTTACAATGCTTCACTCAGAGCAGGTACTCAGGAGATTAGCCAGTGGAACCAATATCCTAATTTTATGGACCAAACACAACCCACTGTTCTGAATTATCAATCTTTGAGCGAACAATCAATCTCTGGTTATGATGAAGTTTTGGGTTCTAGTCCGCGAACCCGGAGCTTCGGATTTGGAAGAGATGATGGTCTCGAAAGAGGGCGAGAAGCGTTGTCGTATGAAGGTACATTGAAGAAATTGGCTCCGCTTGAGGGTGATCAAAGTTTGGACGAGCTGACCACCCTGCAAATGTGTGCAATGAGGAAACGTGCTCTAGCTAGGAAACTACGTCGGAATGAATCAAATTCTGAATTGTCCAATGATTCGAGTGACGCATGCGATGTACCTGACTTGAGTTTTGGGTACGACCAGTGGAGTAATGAAGATTTCAAACATTATGGAATCGTTGGTCAGGATCAATTGAACATTGACCAAGCTGTGACCTGTGGTGAGGGTCAATGGAACGTTGACCAAGATGTGAATTTTGGAGTTAATCGCGTGAACATTAACCAAAATTTGAATTTTGGAGAGGATCAATGGTACACCGACCGGGATTCTATCTATGGAGAGGATCACCGTGGTCCGAACCATGTTGTGGACCACTGGAAAGCAGAAGATGTCAACAAACTAAATTCCGACAATTTGTTACCATCTTCTAAACTGCCAGTACCTGTCCAGCAGCGTCATGGAGAACAATTCCAATCTGCCAGGTTGGATGTAAAGAGGCGTTTGGGACCTTTGAAGTCACCTGGCAGTATAAAGAAGCGCTTGGGACCGTTAAAGTCCTCTGTTAGTGTAAAAAGGCGTTTGGGACCATTGCAATCACCGGGCAGTGTAAAGAAGCATCTCAGGCCTGCTCAAGATGTTCCTAATCTCGAATTCTCAGATTACAAACCTCATGATCAGTACAAATCTCAAATAAGAGAAACCGATGATTTTAGTGGAAGCATTCATCCTCAAGGGGATGACATTCCAAAAGTTAAGGCGAAACCTGCGGAGATGGAACTTCCTGAAGATTCTGAGGAGTTCAAGAGACTGGTGGATAGTGCCTTTTTTAAGCACATTAAGCGTTTGCATGAAAACCCAGCTCTAAGAAGACAATTAACGGGAGGAGGAAGTTTTACTACAAAGTGTTGTGTATGTGCCAGCAACTCGAAAGAGTTTGCAGGTGTGGTGCCCTTGGCAATACATGCCTTCATGTCACGTATGGCTGGTTCCAGAGCAGAGCACTTGGGTTTTCATAAAGCACTTTGTGTGCTAATGGGATGGGACAGTGCATCCGTCTCGAATGGTACATGGGTTCAGCGGTTCTTGCCTGATGCTGAAGCCTTGGCTCTGAAGGAGGACATGGTTATCTTTCCTCCGGTTGTTGTCATTCACAACACTTCCATAGCAAATAAAAATCCGAATAAAAGGGTGATTGTAACTATTGAAGGGCTGGAGGATATTCTTAAAGGTATAGGTTTTGGTGGGGGGAAGGCTAAGGTGTGCCGTGGGAAGCCTGCAAATTACAGCATCTTGGTGGTGACATTTAATGCCACATTTTCTGGTTTTCGGGAAGCAGAAAGGCTTCACAAGCTTTTTGCTGAGAACAAACACGGGAGGGCTGAGTTCCAGCAGATCACCTCAGCTTCCCTCAGCAACAGCAGCAGCGAAGGGAAGCAAAACTCGCTGGAAGACCAAGAAAATTGTTTATATGGCTATGTGGGACTTGCAGAGGATTTTGATAAACTTGAAAGCGAGACCAAGAAGCGGTGTCGGGTGAAGAGCAAGAAGGAAATCCAGGCTACTGCAATTACTTATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

848

Amino Acids

94.35

Weight (kDa)

8.41

Isoelectric Point (pI)

52.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 693 - 821 2.4e-20 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014702)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g29670
malus_domestica MD03G1136700.v1.1
prunus_persica Prupe.6G121100_v2.0.a1 Prupe.6G121100_v2.0.a1
pyrus_communis pycom03g09250
rosa_chinensis RchiOBHm_Chr5g0055031
rosa_laevigata RLG00000034958
rosa_multiflora Rmu_co8511567.1_g000001 Rmu_sc0000925.1_g000013
rosa_roxburghii Rroxscaffold_1G00025460
rosa_rugosa Rorug05G0290200 Rorug05G0290300
rosa_samantha Rh5AG360200 Rh5BG371200 Rh5CG393500 Rh5DG385000
rosa_wichuraiana Rw5G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1892
AatII GACGTC 1 cut(s) 242
Acc36I ACCTGC 5 cut(s) 194, 532, 707, 1735, 1892
AccB1I GGYRCC 2 cut(s) 212, 1909
AccB7I CCANNNNNTGG 1 cut(s) 1330
AccI GTMKAC 1 cut(s) 73
AccII CGCG 5 cut(s) 56, 296, 529, 850, 1233
AccIII TCCGGA 2 cut(s) 335, 358
AclI AACGTT 1 cut(s) 1200
AclWI GGATC 5 cut(s) 539, 552, 1158, 1275, 1314
AcoI YGGCCR 1 cut(s) 188
AcsI RAATTY 8 cut(s) 127, 572, 1039, 1216, 1249, 1255, 1366, 1613
AcuI CTGAAG 3 cut(s) 1767, 2079, 2093
AcyI GRCGYC 2 cut(s) 213, 239
AdeI CACNNNGTG 1 cut(s) 1988
AfeI AGCGCT 1 cut(s) 1502
AfiI CCNNNNNNNGG 9 cut(s) 323, 342, 365, 370, 437, 665, 947, 1330, 2487
AjnI CCWGG 4 cut(s) 364, 1442, 1483, 2517
AluBI AGCT 9 cut(s) 333, 388, 861, 970, 1013, 1172, 1823, 2314, 2366
AluI AGCT 9 cut(s) 333, 388, 861, 970, 1013, 1172, 1823, 2314, 2366
Alw21I GWGCWC 2 cut(s) 1009, 1965
Alw26I GTCTC 7 cut(s) 174, 212, 271, 890, 1762, 2023, 2471
AlwI GGATC 5 cut(s) 539, 552, 1158, 1275, 1314
AlwNI CAGNNNCTG 1 cut(s) 1406
Ama87I CYCGRG 4 cut(s) 17, 28, 85, 532
Aor13HI TCCGGA 2 cut(s) 335, 358
Aor51HI AGCGCT 1 cut(s) 1502
AoxI GGCC 4 cut(s) 188, 504, 686, 1586
ApeKI GCWGC 4 cut(s) 416, 1414, 2382, 2385
ApoI RAATTY 8 cut(s) 127, 572, 1039, 1216, 1249, 1255, 1366, 1613
ArsI GACNNNNNNTTYG 2 cut(s) 1076, 1108
Asp700I GAANNNNTTC 1 cut(s) 2304
AspLEI GCGC 5 cut(s) 56, 215, 529, 531, 1503
AsuC2I CCSGG 8 cut(s) 133, 274, 303, 413, 549, 856, 1286, 1563
AsuNHI GCTAGC 1 cut(s) 268
AvaI CYCGRG 4 cut(s) 17, 28, 85, 532
BaeGI GKGCMC 1 cut(s) 1914
BaeI ACNNNNGTAYC 2 cut(s) 2019, 2052
BamHI GGATCC 1 cut(s) 544
BanI GGYRCC 2 cut(s) 212, 1909
BarI GAAGNNNNNNTAC 2 cut(s) 1843, 1875
BbsI GAAGAC 3 cut(s) 462, 1837, 2418
Bbv12I GWGCWC 2 cut(s) 1009, 1965
BbvCI CCTCAGC 2 cut(s) 2362, 2372
BbvI GCAGC 4 cut(s) 403, 1426, 2394, 2397
BccI CCATC 6 cut(s) 158, 621, 875, 1393, 1729, 1995
BceAI ACGGC 3 cut(s) 269, 491, 2211
BcgI CGANNNNNNTGC 2 cut(s) 2001, 2035
BciT130I CCWGG 4 cut(s) 366, 1444, 1485, 2519
BclI TGATCA 2 cut(s) 952, 1636
BcnI CCSGG 8 cut(s) 133, 274, 303, 413, 549, 856, 1286, 1563
BcoDI GTCTC 7 cut(s) 174, 212, 271, 890, 1762, 2023, 2471
BfaI CTAG 4 cut(s) 269, 843, 1010, 1014
BfoI RGCGCY 2 cut(s) 216, 1504
BfuAI ACCTGC 5 cut(s) 194, 532, 707, 1735, 1892
BisI GCNGC 6 cut(s) 188, 191, 417, 1415, 2383, 2386
BlsI GCNGC 6 cut(s) 189, 192, 418, 1416, 2384, 2387
BmeT110I CYCGRG 4 cut(s) 17, 28, 85, 532
BmsI GCATC 5 cut(s) 604, 1588, 2021, 2044, 2259
BmtI GCTAGC 1 cut(s) 272
BpiI GAAGAC 3 cut(s) 462, 1837, 2418
BpmI CTGGAG 1 cut(s) 2195
Bpu10I CCTNAGC 3 cut(s) 2217, 2362, 2372
BpuEI CTTGAG 4 cut(s) 965, 1108, 1578, 1677
BpuMI CCSGG 8 cut(s) 133, 274, 303, 413, 549, 856, 1286, 1563
BsaAI YACGTR 2 cut(s) 487, 1940
BsaHI GRCGYC 2 cut(s) 213, 239
BsaI GGTCTC 4 cut(s) 212, 271, 890, 2471
BsaJI CCNNGG 7 cut(s) 63, 364, 683, 1312, 1914, 2064, 2227
BsaWI WCCGGW 4 cut(s) 335, 358, 691, 2098
Bsc4I CCNNNNNNNGG 9 cut(s) 323, 342, 365, 370, 437, 665, 947, 1330, 2487
Bse118I RCCGGY 3 cut(s) 199, 215, 617
Bse1I ACTGG 5 cut(s) 736, 1105, 1343, 1400, 1776
Bse3DI GCAATG 2 cut(s) 999, 1550
BseAI TCCGGA 2 cut(s) 335, 358
BseBI CCWGG 4 cut(s) 366, 1444, 1485, 2519
BseDI CCNNGG 7 cut(s) 63, 364, 683, 1312, 1914, 2064, 2227
BseGI GGATG 6 cut(s) 632, 1456, 1687, 1705, 2006, 2012
BseLI CCNNNNNNNGG 9 cut(s) 323, 342, 365, 370, 437, 665, 947, 1330, 2487
BseMI GCAATG 2 cut(s) 999, 1550
BseMII CTCAG 9 cut(s) 724, 737, 1597, 1632, 1746, 2313, 2334, 2376, 2386
BseNI ACTGG 5 cut(s) 736, 1105, 1343, 1400, 1776
BsePI GCGCGC 1 cut(s) 527
BseRI GAGGAG 3 cut(s) 80, 1772, 1860
BseSI GKGCMC 1 cut(s) 1914
BseX3I CGGCCG 1 cut(s) 188
BseXI GCAGC 4 cut(s) 403, 1426, 2394, 2397
BseYI CCCAGC 1 cut(s) 1819
Bsh1236I CGCG 5 cut(s) 56, 296, 529, 850, 1233
Bsh1285I CGRYCG 3 cut(s) 191, 294, 1285
BshFI GGCC 4 cut(s) 190, 506, 688, 1588
BshNI GGYRCC 2 cut(s) 212, 1909
BsiEI CGRYCG 3 cut(s) 191, 294, 1285
BsiHKAI GWGCWC 2 cut(s) 1009, 1965
BsiHKCI CYCGRG 4 cut(s) 17, 28, 85, 532
BslFI GGGAC 8 cut(s) 35, 609, 659, 1482, 1521, 1560, 2018, 2457
BslI CCNNNNNNNGG 9 cut(s) 323, 342, 365, 370, 437, 665, 947, 1330, 2487
BsmAI GTCTC 7 cut(s) 174, 212, 271, 890, 1762, 2023, 2471
BsmBI CGTCTC 1 cut(s) 2023
BsmFI GGGAC 8 cut(s) 35, 609, 659, 1482, 1521, 1560, 2018, 2457
BsmI GAATGC 1 cut(s) 1683
BsnI GGCC 4 cut(s) 190, 506, 688, 1588
Bso31I GGTCTC 4 cut(s) 212, 271, 890, 2471
BsoBI CYCGRG 4 cut(s) 17, 28, 85, 532
Bsp1286I GDGCHC 3 cut(s) 1009, 1914, 1965
Bsp13I TCCGGA 2 cut(s) 335, 358
Bsp143I GATC 7 cut(s) 544, 952, 1150, 1267, 1306, 1636, 2355
BspANI GGCC 4 cut(s) 190, 506, 688, 1588
BspCNI CTCAG 9 cut(s) 723, 736, 1596, 1631, 1747, 2314, 2335, 2375, 2385
BspEI TCCGGA 2 cut(s) 335, 358
BspFNI CGCG 5 cut(s) 56, 296, 529, 850, 1233
BspHI TCATGA 1 cut(s) 1633
BspMI ACCTGC 5 cut(s) 194, 532, 707, 1735, 1892
BspOI GCTAGC 1 cut(s) 272
BspPI GGATC 5 cut(s) 539, 552, 1158, 1275, 1314
BspQI GCTCTTC 1 cut(s) 2495
BspT107I GGYRCC 2 cut(s) 212, 1909
BspTNI GGTCTC 4 cut(s) 212, 271, 890, 2471
BsrDI GCAATG 2 cut(s) 999, 1550
BsrFI RCCGGY 3 cut(s) 199, 215, 617
BsrI ACTGG 5 cut(s) 736, 1105, 1343, 1400, 1776
BssAI RCCGGY 3 cut(s) 199, 215, 617
BssECI CCNNGG 7 cut(s) 63, 364, 683, 1312, 1914, 2064, 2227
BssHII GCGCGC 1 cut(s) 527
BssMI GATC 7 cut(s) 544, 952, 1150, 1267, 1306, 1636, 2355
BssNI GRCGYC 2 cut(s) 213, 239
BssT1I CCWWGG 2 cut(s) 1914, 2064
Bst2UI CCWGG 4 cut(s) 366, 1444, 1485, 2519
Bst4CI ACNGT 5 cut(s) 152, 781, 1313, 1512, 2009
Bst6I CTCTTC 2 cut(s) 868, 2495
BstACI GRCGYC 2 cut(s) 213, 239
BstAPI GCANNNNNTGC 1 cut(s) 1925
BstBAI YACGTR 2 cut(s) 487, 1940
BstC8I GCNNGC 9 cut(s) 217, 221, 270, 390, 529, 1072, 1590, 1882, 2238
BstDSI CCRYGG 2 cut(s) 1312, 2227
BstEII GGTNACC 1 cut(s) 470
BstF5I GGATG 6 cut(s) 632, 1456, 1687, 1705, 2006, 2012
BstFNI CGCG 5 cut(s) 56, 296, 529, 850, 1233
BstH2I RGCGCY 2 cut(s) 216, 1504
BstHHI GCGC 5 cut(s) 56, 215, 529, 531, 1503
BstKTI GATC 7 cut(s) 547, 955, 1153, 1270, 1309, 1639, 2358
BstMAI GTCTC 7 cut(s) 174, 212, 271, 890, 1762, 2023, 2471
BstMBI GATC 7 cut(s) 544, 952, 1150, 1267, 1306, 1636, 2355
BstMCI CGRYCG 3 cut(s) 191, 294, 1285
BstMWI GCNNNNNNNGC 8 cut(s) 1585, 1727, 1801, 1925, 2047, 2311, 2382, 2404
BstNI CCWGG 4 cut(s) 366, 1444, 1485, 2519
BstNSI RCATGY 2 cut(s) 1074, 1928
BstPI GGTNACC 1 cut(s) 470
BstSLI GKGCMC 1 cut(s) 1914
BstUI CGCG 5 cut(s) 56, 296, 529, 850, 1233
BstV1I GCAGC 4 cut(s) 403, 1426, 2394, 2397
BstV2I GAAGAC 3 cut(s) 462, 1837, 2418
BstX2I RGATCY 1 cut(s) 544
BstYI RGATCY 1 cut(s) 544
BstZI CGGCCG 1 cut(s) 188
BsuRI GGCC 4 cut(s) 190, 506, 688, 1588
BtgI CCRYGG 2 cut(s) 1312, 2227
BtgZI GCGATG 1 cut(s) 1089
BtsCI GGATG 6 cut(s) 632, 1456, 1687, 1705, 2006, 2012
BtsI GCAGTG 2 cut(s) 49, 1573
BtsIMutI CAGTG 7 cut(s) 49, 743, 777, 1112, 1336, 1573, 2014
BveI ACCTGC 5 cut(s) 194, 532, 707, 1735, 1892
Cac8I GCNNGC 9 cut(s) 217, 221, 270, 390, 529, 1072, 1590, 1882, 2238
CaiI CAGNNNCTG 1 cut(s) 1406
CciI TCATGA 1 cut(s) 1633
CciNI GCGGCCGC 1 cut(s) 188
CfoI GCGC 5 cut(s) 56, 215, 529, 531, 1503
Cfr10I RCCGGY 3 cut(s) 199, 215, 617
CseI GACGC 2 cut(s) 1076, 1406
DinI GGCGCC 1 cut(s) 214
DpnI GATC 7 cut(s) 546, 954, 1152, 1269, 1308, 1638, 2357
DpnII GATC 7 cut(s) 544, 952, 1150, 1267, 1306, 1636, 2355
DraIII CACNNNGTG 1 cut(s) 1988
EaeI YGGCCR 1 cut(s) 188
EagI CGGCCG 1 cut(s) 188
Eam1104I CTCTTC 2 cut(s) 868, 2495
EarI CTCTTC 2 cut(s) 868, 2495
EciI GGCGGA 3 cut(s) 25, 420, 646
EclXI CGGCCG 1 cut(s) 188
Eco130I CCWWGG 2 cut(s) 1914, 2064
Eco147I AGGCCT 2 cut(s) 688, 1588
Eco31I GGTCTC 4 cut(s) 212, 271, 890, 2471
Eco47III AGCGCT 1 cut(s) 1502
Eco52I CGGCCG 1 cut(s) 188
Eco57I CTGAAG 3 cut(s) 1767, 2079, 2093
Eco88I CYCGRG 4 cut(s) 17, 28, 85, 532
Eco91I GGTNACC 1 cut(s) 470
EcoO109I RGGNCCY 1 cut(s) 1469
EcoO65I GGTNACC 1 cut(s) 470
EcoRI GAATTC 3 cut(s) 127, 572, 1613
EcoRII CCWGG 4 cut(s) 364, 1442, 1483, 2517
EcoT14I CCWWGG 2 cut(s) 1914, 2064
EcoT22I ATGCAT 1 cut(s) 484
EgeI GGCGCC 1 cut(s) 214
EheI GGCGCC 1 cut(s) 214
ErhI CCWWGG 2 cut(s) 1914, 2064
Esp3I CGTCTC 1 cut(s) 2023
FalI AAGNNNNNCTT 2 cut(s) 1487, 1519
FaqI GGGAC 8 cut(s) 35, 609, 659, 1482, 1521, 1560, 2018, 2457
FauI CCCGC 1 cut(s) 289
FauNDI CATATG 1 cut(s) 664
FbaI TGATCA 2 cut(s) 952, 1636
FblI GTMKAC 1 cut(s) 73
Fnu4HI GCNGC 6 cut(s) 188, 191, 417, 1415, 2383, 2386
FokI GGATG 6 cut(s) 639, 1463, 1674, 1712, 1999, 2013
Fsp4HI GCNGC 6 cut(s) 188, 191, 417, 1415, 2383, 2386
FspBI CTAG 4 cut(s) 269, 843, 1010, 1014
GlaI GCGC 5 cut(s) 55, 214, 528, 530, 1502
GluI GCNGC 6 cut(s) 188, 191, 417, 1415, 2383, 2386
GsaI CCCAGC 1 cut(s) 1823
GsuI CTGGAG 1 cut(s) 2195
HaeII RGCGCY 2 cut(s) 216, 1504
HaeIII GGCC 4 cut(s) 190, 506, 688, 1588
HgaI GACGC 2 cut(s) 1076, 1406
HhaI GCGC 5 cut(s) 56, 215, 529, 531, 1503
Hin1I GRCGYC 2 cut(s) 213, 239
Hin6I GCGC 5 cut(s) 54, 213, 527, 529, 1501
HinP1I GCGC 5 cut(s) 54, 213, 527, 529, 1501
HincII GTYRAC 3 cut(s) 74, 1204, 1357
HindII GTYRAC 3 cut(s) 74, 1204, 1357
HindIII AAGCTT 2 cut(s) 386, 2312
Hpy166II GTNNAC 8 cut(s) 74, 373, 697, 1204, 1237, 1278, 1333, 1357
Hpy8I GTNNAC 8 cut(s) 74, 373, 697, 1204, 1237, 1278, 1333, 1357
Hpy99I CGWCG 5 cut(s) 78, 156, 241, 244, 1029
HpyAV CCTTC 7 cut(s) 911, 1939, 2068, 2162, 2206, 2384, 2503
HpyCH4III ACNGT 5 cut(s) 152, 781, 1313, 1512, 2009
HpyCH4IV ACGT 7 cut(s) 76, 239, 486, 1003, 1024, 1200, 1939
HpyF10VI GCNNNNNNNGC 8 cut(s) 1585, 1727, 1801, 1925, 2047, 2311, 2382, 2404
HpySE526I ACGT 7 cut(s) 76, 239, 486, 1003, 1024, 1200, 1939
Hsp92I GRCGYC 2 cut(s) 213, 239
HspAI GCGC 5 cut(s) 54, 213, 527, 529, 1501
KasI GGCGCC 1 cut(s) 212
Kpn2I TCCGGA 2 cut(s) 335, 358
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
Ksp22I TGATCA 2 cut(s) 952, 1636
Kzo9I GATC 7 cut(s) 544, 952, 1150, 1267, 1306, 1636, 2355
LguI GCTCTTC 1 cut(s) 2495
LmnI GCTCC 3 cut(s) 338, 858, 943
Lsp1109I GCAGC 4 cut(s) 403, 1426, 2394, 2397
LweI GCATC 5 cut(s) 604, 1588, 2021, 2044, 2259
MaeI CTAG 4 cut(s) 269, 843, 1010, 1014
MaeII ACGT 7 cut(s) 76, 239, 486, 1003, 1024, 1200, 1939
MaeIII GTNAC 8 cut(s) 470, 1064, 1174, 1380, 1479, 1935, 2158, 2260
MalI GATC 7 cut(s) 546, 954, 1152, 1269, 1308, 1638, 2357
MboI GATC 7 cut(s) 544, 952, 1150, 1267, 1306, 1636, 2355
MfeI CAATTG 1 cut(s) 1154
MflI RGATCY 1 cut(s) 544
MhlI GDGCHC 3 cut(s) 1009, 1914, 1965
Mly113I GGCGCC 1 cut(s) 213
MlyI GAGTC 2 cut(s) 40, 79
MmeI TCCRAC 4 cut(s) 287, 1007, 1395, 1428
Mph1103I ATGCAT 1 cut(s) 484
MroI TCCGGA 2 cut(s) 335, 358
MroNI GCCGGC 1 cut(s) 215
MroXI GAANNNNTTC 1 cut(s) 2304
MseI TTAA 9 cut(s) 1227, 1242, 1514, 1716, 1791, 1800, 1838, 2187, 2268
MslI CAYNNNNRTG 1 cut(s) 2255
MspA1I CMGCKG 1 cut(s) 2041
MunI CAATTG 1 cut(s) 1154
Mva1269I GAATGC 1 cut(s) 1683
MvaI CCWGG 4 cut(s) 366, 1444, 1485, 2519
MvnI CGCG 5 cut(s) 56, 296, 529, 850, 1233
MwoI GCNNNNNNNGC 8 cut(s) 1585, 1727, 1801, 1925, 2047, 2311, 2382, 2404
NaeI GCCGGC 1 cut(s) 217
NarI GGCGCC 1 cut(s) 213
NciI CCSGG 8 cut(s) 133, 274, 303, 413, 549, 856, 1286, 1563
NdeI CATATG 1 cut(s) 664
NdeII GATC 7 cut(s) 544, 952, 1150, 1267, 1306, 1636, 2355
NgoMIV GCCGGC 1 cut(s) 215
NheI GCTAGC 1 cut(s) 268
NmeAIII GCCGAG 2 cut(s) 88, 189
NmuCI GTSAC 6 cut(s) 470, 1064, 1174, 1479, 1935, 2260
NotI GCGGCCGC 1 cut(s) 188
NsiI ATGCAT 1 cut(s) 484
NspI RCATGY 2 cut(s) 1074, 1928
PaeI GCATGC 1 cut(s) 1074
PaeR7I CTCGAG 1 cut(s) 532
PagI TCATGA 1 cut(s) 1633
PaqCI CACCTGC 1 cut(s) 1892
PauI GCGCGC 1 cut(s) 527
PceI AGGCCT 2 cut(s) 688, 1588
PciSI GCTCTTC 1 cut(s) 2495
PcsI WCGNNNNNNNCGW 1 cut(s) 236
PctI GAATGC 1 cut(s) 1683
PdiI GCCGGC 1 cut(s) 217
PdmI GAANNNNTTC 1 cut(s) 2304
PfeI GAWTC 9 cut(s) 233, 298, 408, 634, 1034, 1058, 1137, 1289, 1751
PflMI CCANNNNNTGG 1 cut(s) 1330
PfoI TCCNGGA 1 cut(s) 131
PkrI GCNGC 6 cut(s) 189, 192, 418, 1416, 2384, 2387
PleI GAGTC 2 cut(s) 39, 78
PluTI GGCGCC 1 cut(s) 216
PpsI GAGTC 2 cut(s) 39, 78
Ppu21I YACGTR 2 cut(s) 487, 1940
PpuMI RGGWCCY 1 cut(s) 1469
Psp1406I AACGTT 1 cut(s) 1200
Psp5II RGGWCCY 1 cut(s) 1469
Psp6I CCWGG 4 cut(s) 364, 1442, 1483, 2517
PspEI GGTNACC 1 cut(s) 470
PspFI CCCAGC 1 cut(s) 1819
PspGI CCWGG 4 cut(s) 364, 1442, 1483, 2517
PspPPI RGGWCCY 1 cut(s) 1469
PspXI VCTCGAGB 1 cut(s) 532
PsrI GAACNNNNNNTAC 2 cut(s) 1933, 1965
PstNI CAGNNNCTG 1 cut(s) 1406
PsuI RGATCY 1 cut(s) 544
PteI GCGCGC 1 cut(s) 527
RseI CAYNNNNRTG 1 cut(s) 2255
SalI GTCGAC 1 cut(s) 72
SapI GCTCTTC 1 cut(s) 2495
SaqAI TTAA 9 cut(s) 1227, 1242, 1514, 1716, 1791, 1800, 1838, 2187, 2268
SatI GCNGC 6 cut(s) 188, 191, 417, 1415, 2383, 2386
Sau3AI GATC 7 cut(s) 544, 952, 1150, 1267, 1306, 1636, 2355
SchI GAGTC 2 cut(s) 40, 79
SduI GDGCHC 3 cut(s) 1009, 1914, 1965
SfaNI GCATC 5 cut(s) 604, 1588, 2021, 2044, 2259
SfoI GGCGCC 1 cut(s) 214
Sfr274I CTCGAG 1 cut(s) 532
SlaI CTCGAG 1 cut(s) 532
SmiMI CAYNNNNRTG 1 cut(s) 2255
SmlI CTYRAG 5 cut(s) 532, 944, 1087, 1593, 1692
SmoI CTYRAG 5 cut(s) 532, 944, 1087, 1593, 1692
SphI GCATGC 1 cut(s) 1074
SseBI AGGCCT 2 cut(s) 688, 1588
SspDI GGCGCC 1 cut(s) 212
SspMI CTAG 4 cut(s) 269, 843, 1010, 1014
StuI AGGCCT 2 cut(s) 688, 1588
StyI CCWWGG 2 cut(s) 1914, 2064
TaaI ACNGT 5 cut(s) 152, 781, 1313, 1512, 2009
TaiI ACGT 7 cut(s) 79, 242, 489, 1006, 1027, 1203, 1942
TatI WGTACW 1 cut(s) 1641
TauI GCSGC 2 cut(s) 190, 193
TfiI GAWTC 9 cut(s) 233, 298, 408, 634, 1034, 1058, 1137, 1289, 1751
Tru1I TTAA 9 cut(s) 1227, 1242, 1514, 1716, 1791, 1800, 1838, 2187, 2268
Tru9I TTAA 9 cut(s) 1227, 1242, 1514, 1716, 1791, 1800, 1838, 2187, 2268
TscAI CASTG 7 cut(s) 56, 743, 784, 1112, 1343, 1573, 2014
TseFI GTSAC 6 cut(s) 470, 1064, 1174, 1479, 1935, 2260
TseI GCWGC 4 cut(s) 416, 1414, 2382, 2385
Tsp45I GTSAC 6 cut(s) 470, 1064, 1174, 1479, 1935, 2260
TspGWI ACGGA 2 cut(s) 534, 2005
TspRI CASTG 7 cut(s) 56, 743, 784, 1112, 1343, 1573, 2014
Van91I CCANNNNNTGG 1 cut(s) 1330
XapI RAATTY 8 cut(s) 127, 572, 1039, 1216, 1249, 1255, 1366, 1613
XceI RCATGY 2 cut(s) 1074, 1928
XcmI CCANNNNNNNNNTGG 1 cut(s) 1418
XhoI CTCGAG 1 cut(s) 532
XmiI GTMKAC 1 cut(s) 73
XmnI GAANNNNTTC 1 cut(s) 2304
XspI CTAG 4 cut(s) 269, 843, 1010, 1014
ZraI GACGTC 1 cut(s) 240
Zsp2I ATGCAT 1 cut(s) 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.