FvH4_3g30490

Ribosomal protein S14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0006836
Physical Location & Seq
Reverse (-)
23762490 .. 23763239
750 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g30490.t1

Sequence Viewer

Length: 330 bp
ATGTCGGTATGCAGAGAAAACGCGAACATCAGAGACAACCACCGGCGATTACTGGCGGAGAAATTCGAGCTGCGGCGGAACCTGTACAAGGCGCTGTGCAAAGACACGAGCCTCCCCGACGATTTACGCGAGGAGAATCGCTACAAGCTCTCGAAGCTTCCTCGGAACAGCTCCTTCACCCGAATCCGGAACCGGTGCATCTTCTCCGGCCGAGGCCGCGCCGTCTACGAGACCTTCCGCATGTCCCGTCTCTTCTCCCGCCTCCAAGGGCATGCTCAACGGCGTCAAGAAAGCCTCCTGGTAGCCAAACGGATTGGTGTGAGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S14

Protein Analysis

110

Amino Acids

12.97

Weight (kDa)

11.17

Isoelectric Point (pI)

61.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S14 PF00253 49 - 90 2.4e-13 Ribosomal protein S14p/S29e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 225
AccII CGCG 3 cut(s) 23, 129, 219
AccIII TCCGGA 1 cut(s) 186
AciI CCGC 6 cut(s) 56, 73, 76, 217, 238, 259
AcoI YGGCCR 1 cut(s) 208
AcsI RAATTY 1 cut(s) 62
AcyI GRCGYC 1 cut(s) 283
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 2 cut(s) 88, 186
AgeI ACCGGT 1 cut(s) 192
AjnI CCWGG 1 cut(s) 297
AluBI AGCT 4 cut(s) 70, 148, 157, 171
AluI AGCT 4 cut(s) 70, 148, 157, 171
Alw26I GTCTC 3 cut(s) 27, 224, 254
Aor13HI TCCGGA 1 cut(s) 186
AoxI GGCC 2 cut(s) 208, 214
ApeKI GCWGC 1 cut(s) 70
ApoI RAATTY 1 cut(s) 62
AsiGI ACCGGT 1 cut(s) 192
AspLEI GCGC 2 cut(s) 94, 221
AsuHPI GGTGA 1 cut(s) 169
BauI CACGAG 1 cut(s) 106
BbvI GCAGC 1 cut(s) 57
BceAI ACGGC 2 cut(s) 206, 296
BciT130I CCWGG 1 cut(s) 299
BcoDI GTCTC 3 cut(s) 27, 224, 254
BfoI RGCGCY 1 cut(s) 95
BisI GCNGC 3 cut(s) 71, 74, 217
BlsI GCNGC 3 cut(s) 72, 75, 218
Bme1390I CCNGG 1 cut(s) 299
BmiI GGNNCC 2 cut(s) 80, 191
BmrFI CCNGG 1 cut(s) 299
BmsI GCATC 1 cut(s) 207
BsaHI GRCGYC 1 cut(s) 283
BsaI GGTCTC 1 cut(s) 224
BsaJI CCNNGG 3 cut(s) 161, 211, 265
BsaWI WCCGGW 2 cut(s) 186, 192
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 186
Bse118I RCCGGY 2 cut(s) 42, 192
Bse1I ACTGG 1 cut(s) 57
BseAI TCCGGA 1 cut(s) 186
BseBI CCWGG 1 cut(s) 299
BseDI CCNNGG 3 cut(s) 161, 211, 265
BseLI CCNNNNNNNGG 2 cut(s) 88, 186
BseNI ACTGG 1 cut(s) 57
BseRI GAGGAG 1 cut(s) 146
BseX3I CGGCCG 1 cut(s) 208
BseXI GCAGC 1 cut(s) 57
Bsh1236I CGCG 3 cut(s) 23, 129, 219
Bsh1285I CGRYCG 1 cut(s) 211
BshFI GGCC 2 cut(s) 210, 216
BshTI ACCGGT 1 cut(s) 192
BsiEI CGRYCG 1 cut(s) 211
BsiSI CCGG 4 cut(s) 43, 187, 193, 207
BslFI GGGAC 1 cut(s) 229
BslI CCNNNNNNNGG 2 cut(s) 88, 186
BsmAI GTCTC 3 cut(s) 27, 224, 254
BsmBI CGTCTC 1 cut(s) 254
BsmFI GGGAC 1 cut(s) 229
BsnI GGCC 2 cut(s) 210, 216
Bso31I GGTCTC 1 cut(s) 224
Bsp13I TCCGGA 1 cut(s) 186
Bsp1407I TGTACA 1 cut(s) 84
BspACI CCGC 6 cut(s) 56, 73, 76, 217, 238, 259
BspANI GGCC 2 cut(s) 210, 216
BspEI TCCGGA 1 cut(s) 186
BspFNI CGCG 3 cut(s) 23, 129, 219
BspLI GGNNCC 2 cut(s) 80, 191
BspTNI GGTCTC 1 cut(s) 224
BsrFI RCCGGY 2 cut(s) 42, 192
BsrGI TGTACA 1 cut(s) 84
BsrI ACTGG 1 cut(s) 57
BssAI RCCGGY 2 cut(s) 42, 192
BssECI CCNNGG 3 cut(s) 161, 211, 265
BssNI GRCGYC 1 cut(s) 283
BssSI CACGAG 1 cut(s) 106
BssT1I CCWWGG 1 cut(s) 265
Bst2BI CACGAG 1 cut(s) 106
Bst2UI CCWGG 1 cut(s) 299
Bst6I CTCTTC 1 cut(s) 257
BstACI GRCGYC 1 cut(s) 283
BstAUI TGTACA 1 cut(s) 84
BstC8I GCNNGC 1 cut(s) 273
BstENI CCTNNNNNAGG 1 cut(s) 86
BstFNI CGCG 3 cut(s) 23, 129, 219
BstH2I RGCGCY 1 cut(s) 95
BstHHI GCGC 2 cut(s) 94, 221
BstMAI GTCTC 3 cut(s) 27, 224, 254
BstMCI CGRYCG 1 cut(s) 211
BstMWI GCNNNNNNNGC 2 cut(s) 154, 216
BstNI CCWGG 1 cut(s) 299
BstNSI RCATGY 2 cut(s) 244, 275
BstSCI CCNGG 1 cut(s) 297
BstUI CGCG 3 cut(s) 23, 129, 219
BstV1I GCAGC 1 cut(s) 57
BstZI CGGCCG 1 cut(s) 208
BsuRI GGCC 2 cut(s) 210, 216
Cac8I GCNNGC 1 cut(s) 273
CfoI GCGC 2 cut(s) 94, 221
Cfr10I RCCGGY 2 cut(s) 42, 192
CseI GACGC 1 cut(s) 272
Csp6I GTAC 1 cut(s) 85
CspAI ACCGGT 1 cut(s) 192
CviAII CATG 2 cut(s) 241, 272
CviJI RGCY 9 cut(s) 70, 111, 148, 157, 171, 210, 216, 294, 305
CviKI_1 RGCY 9 cut(s) 70, 111, 148, 157, 171, 210, 216, 294, 305
CviQI GTAC 1 cut(s) 85
EaeI YGGCCR 1 cut(s) 208
EagI CGGCCG 1 cut(s) 208
Eam1104I CTCTTC 1 cut(s) 257
EarI CTCTTC 1 cut(s) 257
EciI GGCGGA 2 cut(s) 71, 91
EclXI CGGCCG 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 265
Eco31I GGTCTC 1 cut(s) 224
Eco52I CGGCCG 1 cut(s) 208
EcoNI CCTNNNNNAGG 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 297
EcoT14I CCWWGG 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 265
Esp3I CGTCTC 1 cut(s) 254
FaeI CATG 2 cut(s) 244, 275
FaiI YATR 3 cut(s) 10, 242, 273
FaqI GGGAC 1 cut(s) 229
FatI CATG 2 cut(s) 240, 271
FauI CCCGC 1 cut(s) 266
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 3 cut(s) 71, 74, 217
Fsp4HI GCNGC 3 cut(s) 71, 74, 217
GlaI GCGC 2 cut(s) 93, 220
GluI GCNGC 3 cut(s) 71, 74, 217
HaeII RGCGCY 1 cut(s) 95
HaeIII GGCC 2 cut(s) 210, 216
HapII CCGG 4 cut(s) 43, 187, 193, 207
HgaI GACGC 1 cut(s) 272
HhaI GCGC 2 cut(s) 94, 221
Hin1I GRCGYC 1 cut(s) 283
Hin1II CATG 2 cut(s) 244, 275
Hin6I GCGC 2 cut(s) 92, 219
HinP1I GCGC 2 cut(s) 92, 219
HindIII AAGCTT 1 cut(s) 155
HinfI GANTC 2 cut(s) 136, 183
HpaII CCGG 4 cut(s) 43, 187, 193, 207
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 2 cut(s) 32, 165
Hpy188III TCNNGA 3 cut(s) 151, 187, 287
Hpy8I GTNNAC 1 cut(s) 226
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 2 cut(s) 184, 244
HpyCH4V TGCA 3 cut(s) 12, 99, 198
HpyF10VI GCNNNNNNNGC 2 cut(s) 154, 216
Hsp92I GRCGYC 1 cut(s) 283
Hsp92II CATG 2 cut(s) 244, 275
HspAI GCGC 2 cut(s) 92, 219
Kpn2I TCCGGA 1 cut(s) 186
LmnI GCTCC 1 cut(s) 176
LpnPI CCDG 8 cut(s) 38, 56, 95, 200, 206, 220, 284, 311
Lsp1109I GCAGC 1 cut(s) 57
LweI GCATC 1 cut(s) 207
MboII GAAGA 2 cut(s) 193, 244
MluCI AATT 1 cut(s) 62
MnlI CCTC 6 cut(s) 122, 124, 171, 206, 272, 305
MroI TCCGGA 1 cut(s) 186
MspI CCGG 4 cut(s) 43, 187, 193, 207
MspR9I CCNGG 1 cut(s) 299
MvaI CCWGG 1 cut(s) 299
MvnI CGCG 3 cut(s) 23, 129, 219
MwoI GCNNNNNNNGC 2 cut(s) 154, 216
NlaIII CATG 2 cut(s) 244, 275
NlaIV GGNNCC 2 cut(s) 80, 191
NmeAIII GCCGAG 1 cut(s) 236
NspI RCATGY 2 cut(s) 244, 275
PaeI GCATGC 1 cut(s) 275
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PfeI GAWTC 2 cut(s) 136, 183
PinAI ACCGGT 1 cut(s) 192
PkrI GCNGC 3 cut(s) 72, 75, 218
Psp6I CCWGG 1 cut(s) 297
PspGI CCWGG 1 cut(s) 297
PspN4I GGNNCC 2 cut(s) 80, 191
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SatI GCNGC 3 cut(s) 71, 74, 217
ScrFI CCNGG 1 cut(s) 299
SetI ASST 6 cut(s) 72, 84, 150, 159, 173, 236
SfaNI GCATC 1 cut(s) 207
SgrAI CRCCGGYG 1 cut(s) 42
SphI GCATGC 1 cut(s) 275
Sse9I AATT 1 cut(s) 62
SsiI CCGC 6 cut(s) 56, 73, 76, 217, 238, 259
StyD4I CCNGG 1 cut(s) 297
StyI CCWWGG 1 cut(s) 265
TaqI TCGA 2 cut(s) 66, 152
TasI AATT 1 cut(s) 62
TatI WGTACW 1 cut(s) 84
TauI GCSGC 2 cut(s) 76, 219
TfiI GAWTC 2 cut(s) 136, 183
TseI GCWGC 1 cut(s) 70
TspGWI ACGGA 1 cut(s) 325
XagI CCTNNNNNAGG 1 cut(s) 86
XapI RAATTY 1 cut(s) 62
XceI RCATGY 2 cut(s) 244, 275
XmiI GTMKAC 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.