Rw1G011090

Ribosomal protein S14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006836
Physical Location & Seq
Reverse (-)
25017734 .. 25021624
3891 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G011090.1

Sequence Viewer

Length: 312 bp
ATGTCAGTTTGCCGAGAAAACGCCAACATCCGAGACAACCACCGGCGGTTGCTGGCCGAGAAATTCGAGCTCCGGCGGAACCTGTACAAGGCGCTGTGCAAAGACACGAGCCTCCCCGACGAGTTGCGTGAGGAAAACCGGTACAAGCTCTCCAAGCTTCCTCGAAACAGCTCCTTCACTCGAATCAGGAACCGCTGCATCTTCTCCGGCCGAGGCCGCTCCGTCTACGAGACCTTCCGTATGTCTCGTATCGTCTTCCGCGCTCTCGCCTCCAAGGGCATGCTCAACGGCGTCAAGAAAGCGTCCTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S14

Protein Analysis

103

Amino Acids

12.23

Weight (kDa)

10.9

Isoelectric Point (pI)

46.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S14 PF00253 49 - 102 9.1e-24 Ribosomal protein S14p/S29e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 219
AccI GTMKAC 1 cut(s) 225
AccII CGCG 1 cut(s) 261
AciI CCGC 5 cut(s) 46, 76, 193, 217, 259
AcoI YGGCCR 2 cut(s) 54, 208
AcsI RAATTY 1 cut(s) 62
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 2 cut(s) 86, 143
AfiI CCNNNNNNNGG 1 cut(s) 88
AgeI ACCGGT 1 cut(s) 138
AjnI CCWGG 1 cut(s) 305
AluBI AGCT 4 cut(s) 70, 148, 157, 171
AluI AGCT 4 cut(s) 70, 148, 157, 171
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 3 cut(s) 27, 224, 249
AoxI GGCC 3 cut(s) 54, 208, 214
ApeKI GCWGC 1 cut(s) 195
ApoI RAATTY 1 cut(s) 62
AsiGI ACCGGT 1 cut(s) 138
AspLEI GCGC 2 cut(s) 94, 263
BanII GRGCYC 1 cut(s) 72
BauI CACGAG 1 cut(s) 106
BbsI GAAGAC 1 cut(s) 247
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 1 cut(s) 182
BceAI ACGGC 1 cut(s) 304
BciT130I CCWGG 1 cut(s) 307
BcoDI GTCTC 3 cut(s) 27, 224, 249
BfoI RGCGCY 1 cut(s) 95
BisI GCNGC 2 cut(s) 196, 217
BlsI GCNGC 2 cut(s) 197, 218
Bme1390I CCNGG 1 cut(s) 307
BmiI GGNNCC 2 cut(s) 80, 191
BmrFI CCNGG 1 cut(s) 307
BmsI GCATC 1 cut(s) 207
BpiI GAAGAC 1 cut(s) 247
BsaHI GRCGYC 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 224
BsaJI CCNNGG 2 cut(s) 211, 273
BsaWI WCCGGW 1 cut(s) 138
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse118I RCCGGY 2 cut(s) 42, 138
BseBI CCWGG 1 cut(s) 307
BseDI CCNNGG 2 cut(s) 211, 273
BseGI GGATG 1 cut(s) 27
BseLI CCNNNNNNNGG 1 cut(s) 88
BseX3I CGGCCG 1 cut(s) 208
BseXI GCAGC 1 cut(s) 182
Bsh1236I CGCG 1 cut(s) 261
Bsh1285I CGRYCG 1 cut(s) 211
BshFI GGCC 3 cut(s) 56, 210, 216
BshTI ACCGGT 1 cut(s) 138
BsiEI CGRYCG 1 cut(s) 211
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 4 cut(s) 43, 73, 139, 207
BslI CCNNNNNNNGG 1 cut(s) 88
BsmAI GTCTC 3 cut(s) 27, 224, 249
BsnI GGCC 3 cut(s) 56, 210, 216
Bso31I GGTCTC 1 cut(s) 224
Bsp1286I GDGCHC 1 cut(s) 72
Bsp1407I TGTACA 1 cut(s) 84
BspACI CCGC 5 cut(s) 46, 76, 193, 217, 259
BspANI GGCC 3 cut(s) 56, 210, 216
BspFNI CGCG 1 cut(s) 261
BspLI GGNNCC 2 cut(s) 80, 191
BspTNI GGTCTC 1 cut(s) 224
BsrBI CCGCTC 1 cut(s) 219
BsrFI RCCGGY 2 cut(s) 42, 138
BsrGI TGTACA 1 cut(s) 84
BssAI RCCGGY 2 cut(s) 42, 138
BssECI CCNNGG 2 cut(s) 211, 273
BssNI GRCGYC 1 cut(s) 291
BssSI CACGAG 1 cut(s) 106
BssT1I CCWWGG 1 cut(s) 273
Bst2BI CACGAG 1 cut(s) 106
Bst2UI CCWGG 1 cut(s) 307
BstACI GRCGYC 1 cut(s) 291
BstAUI TGTACA 1 cut(s) 84
BstC8I GCNNGC 2 cut(s) 54, 281
BstENI CCTNNNNNAGG 1 cut(s) 86
BstF5I GGATG 1 cut(s) 27
BstFNI CGCG 1 cut(s) 261
BstH2I RGCGCY 1 cut(s) 95
BstHHI GCGC 2 cut(s) 94, 263
BstMAI GTCTC 3 cut(s) 27, 224, 249
BstMCI CGRYCG 1 cut(s) 211
BstMWI GCNNNNNNNGC 2 cut(s) 154, 216
BstNI CCWGG 1 cut(s) 307
BstNSI RCATGY 1 cut(s) 283
BstSCI CCNGG 1 cut(s) 305
BstUI CGCG 1 cut(s) 261
BstV1I GCAGC 1 cut(s) 182
BstV2I GAAGAC 1 cut(s) 247
BstZI CGGCCG 1 cut(s) 208
BsuRI GGCC 3 cut(s) 56, 210, 216
BtsCI GGATG 1 cut(s) 27
Cac8I GCNNGC 2 cut(s) 54, 281
CfoI GCGC 2 cut(s) 94, 263
Cfr10I RCCGGY 2 cut(s) 42, 138
CseI GACGC 2 cut(s) 280, 291
Csp6I GTAC 2 cut(s) 85, 142
CspAI ACCGGT 1 cut(s) 138
CviAII CATG 1 cut(s) 280
CviJI RGCY 8 cut(s) 56, 70, 111, 148, 157, 171, 210, 216
CviKI_1 RGCY 8 cut(s) 56, 70, 111, 148, 157, 171, 210, 216
CviQI GTAC 2 cut(s) 85, 142
EaeI YGGCCR 2 cut(s) 54, 208
EagI CGGCCG 1 cut(s) 208
EciI GGCGGA 1 cut(s) 91
Ecl136II GAGCTC 1 cut(s) 70
EclXI CGGCCG 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 273
Eco24I GRGCYC 1 cut(s) 72
Eco31I GGTCTC 1 cut(s) 224
Eco52I CGGCCG 1 cut(s) 208
Eco53kI GAGCTC 1 cut(s) 70
EcoICRI GAGCTC 1 cut(s) 70
EcoNI CCTNNNNNAGG 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 305
EcoT14I CCWWGG 1 cut(s) 273
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 1 cut(s) 283
FaiI YATR 2 cut(s) 242, 281
FatI CATG 1 cut(s) 279
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 2 cut(s) 196, 217
FokI GGATG 1 cut(s) 14
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 2 cut(s) 196, 217
GlaI GCGC 2 cut(s) 93, 262
GluI GCNGC 2 cut(s) 196, 217
HaeII RGCGCY 1 cut(s) 95
HaeIII GGCC 3 cut(s) 56, 210, 216
HapII CCGG 4 cut(s) 43, 73, 139, 207
HgaI GACGC 2 cut(s) 280, 291
HhaI GCGC 2 cut(s) 94, 263
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 1 cut(s) 283
Hin6I GCGC 2 cut(s) 92, 261
HinP1I GCGC 2 cut(s) 92, 261
HindIII AAGCTT 1 cut(s) 155
HinfI GANTC 1 cut(s) 183
HpaII CCGG 4 cut(s) 43, 73, 139, 207
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 1 cut(s) 32
Hpy188III TCNNGA 2 cut(s) 187, 295
Hpy8I GTNNAC 1 cut(s) 226
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 2 cut(s) 184, 244
HpyCH4V TGCA 2 cut(s) 99, 198
HpyF10VI GCNNNNNNNGC 2 cut(s) 154, 216
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 1 cut(s) 283
HspAI GCGC 2 cut(s) 92, 261
LmnI GCTCC 3 cut(s) 75, 176, 224
LpnPI CCDG 8 cut(s) 38, 56, 86, 95, 152, 172, 220, 292
Lsp1109I GCAGC 1 cut(s) 182
LweI GCATC 1 cut(s) 207
MbiI CCGCTC 1 cut(s) 219
MboII GAAGA 2 cut(s) 193, 247
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 1 cut(s) 62
MnlI CCTC 5 cut(s) 122, 124, 171, 206, 280
MspA1I CMGCKG 1 cut(s) 195
MspI CCGG 4 cut(s) 43, 73, 139, 207
MspR9I CCNGG 1 cut(s) 307
MvaI CCWGG 1 cut(s) 307
MvnI CGCG 1 cut(s) 261
MwoI GCNNNNNNNGC 2 cut(s) 154, 216
NlaIII CATG 1 cut(s) 283
NlaIV GGNNCC 2 cut(s) 80, 191
NmeAIII GCCGAG 3 cut(s) 38, 82, 236
NspI RCATGY 1 cut(s) 283
PaeI GCATGC 1 cut(s) 283
PfeI GAWTC 1 cut(s) 183
PinAI ACCGGT 1 cut(s) 138
PkrI GCNGC 2 cut(s) 197, 218
Psp124BI GAGCTC 1 cut(s) 72
Psp6I CCWGG 1 cut(s) 305
PspGI CCWGG 1 cut(s) 305
PspN4I GGNNCC 2 cut(s) 80, 191
RsaI GTAC 2 cut(s) 86, 143
RsaNI GTAC 2 cut(s) 85, 142
SacI GAGCTC 1 cut(s) 72
SatI GCNGC 2 cut(s) 196, 217
ScrFI CCNGG 1 cut(s) 307
SduI GDGCHC 1 cut(s) 72
SetI ASST 6 cut(s) 72, 84, 150, 159, 173, 236
SfaNI GCATC 1 cut(s) 207
SgrAI CRCCGGYG 1 cut(s) 42
SphI GCATGC 1 cut(s) 283
Sse9I AATT 1 cut(s) 62
SsiI CCGC 5 cut(s) 46, 76, 193, 217, 259
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 1 cut(s) 305
StyI CCWWGG 1 cut(s) 273
TaqI TCGA 3 cut(s) 66, 163, 181
TasI AATT 1 cut(s) 62
TatI WGTACW 1 cut(s) 84
TauI GCSGC 1 cut(s) 219
TfiI GAWTC 1 cut(s) 183
TseI GCWGC 1 cut(s) 195
TspGWI ACGGA 2 cut(s) 211, 227
XagI CCTNNNNNAGG 1 cut(s) 86
XapI RAATTY 1 cut(s) 62
XceI RCATGY 1 cut(s) 283
XmiI GTMKAC 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.