MD07G1070600.v1.1

Ribosomal protein S14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006836
Physical Location & Seq
Reverse (-)
6672417 .. 6676914
4498 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1070600.v1.1.491

Sequence Viewer

Length: 312 bp
ATGTCAGTGTCTGAGGAAAATGCCAACATACGGGACCACCACCGCCGCTTGCTGGCGGCGAAATACGAGCTGAGACGGAACCTGTACAAGGCCCTCTGCAAGGACCCAAGCCTTCCCAGCGATCTGCGCGAGGAGAACCGCTTCAAGCTCTCCAAGCTGCCCCGGAACAGCTCCTTCACGCGCGTTAGGAACCGCTGCATCTTCTCTGGCCGCGCACGCGCCGTCTACGAGACCTTCCGTATGTCCCGTATCGTTTTCCGTTCTCTTGCCTCCAAAGGCATGCTCAATGGCGTGAAGAAAGCGTCTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S14

Protein Analysis

104

Amino Acids

12.1

Weight (kDa)

11.11

Isoelectric Point (pI)

56.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S14 PF00253 49 - 102 7.7e-24 Ribosomal protein S14p/S29e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 225
AccII CGCG 5 cut(s) 129, 181, 183, 213, 219
AciI CCGC 6 cut(s) 43, 46, 56, 139, 193, 211
AcoI YGGCCR 1 cut(s) 208
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 4 cut(s) 30, 52, 88, 100
AgsI TTSAA 1 cut(s) 145
AluBI AGCT 4 cut(s) 70, 148, 157, 171
AluI AGCT 4 cut(s) 70, 148, 157, 171
Alw26I GTCTC 2 cut(s) 67, 224
AlwNI CAGNNNCTG 1 cut(s) 11
AoxI GGCC 2 cut(s) 90, 208
ApeKI GCWGC 2 cut(s) 157, 195
Asp700I GAANNNNTTC 1 cut(s) 140
AspLEI GCGC 4 cut(s) 129, 183, 215, 221
AspS9I GGNCC 3 cut(s) 34, 91, 103
AsuC2I CCSGG 1 cut(s) 163
AvaII GGWCC 2 cut(s) 34, 103
BbvI GCAGC 2 cut(s) 144, 182
BceAI ACGGC 1 cut(s) 206
BcnI CCSGG 1 cut(s) 163
BcoDI GTCTC 2 cut(s) 67, 224
BisI GCNGC 5 cut(s) 46, 57, 158, 196, 211
BlsI GCNGC 5 cut(s) 47, 58, 159, 197, 212
Bme1390I CCNGG 1 cut(s) 163
Bme18I GGWCC 2 cut(s) 34, 103
BmgT120I GGNCC 3 cut(s) 34, 91, 103
BmiI GGNNCC 4 cut(s) 35, 80, 105, 191
BmrFI CCNGG 1 cut(s) 163
BmsI GCATC 1 cut(s) 207
BpuMI CCSGG 1 cut(s) 163
BsaI GGTCTC 1 cut(s) 224
BsaJI CCNNGG 1 cut(s) 161
Bsc4I CCNNNNNNNGG 4 cut(s) 30, 52, 88, 100
BseDI CCNNGG 1 cut(s) 161
BseLI CCNNNNNNNGG 4 cut(s) 30, 52, 88, 100
BseMII CTCAG 1 cut(s) 62
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 2 cut(s) 144, 182
BseYI CCCAGC 1 cut(s) 116
Bsh1236I CGCG 5 cut(s) 129, 181, 183, 213, 219
BshFI GGCC 2 cut(s) 92, 210
BsiSI CCGG 1 cut(s) 163
BslFI GGGAC 2 cut(s) 47, 229
BslI CCNNNNNNNGG 4 cut(s) 30, 52, 88, 100
BsmAI GTCTC 2 cut(s) 67, 224
BsmBI CGTCTC 1 cut(s) 67
BsmFI GGGAC 2 cut(s) 47, 229
BsnI GGCC 2 cut(s) 92, 210
Bso31I GGTCTC 1 cut(s) 224
Bsp1407I TGTACA 1 cut(s) 84
Bsp143I GATC 1 cut(s) 121
BspACI CCGC 6 cut(s) 43, 46, 56, 139, 193, 211
BspANI GGCC 2 cut(s) 92, 210
BspCNI CTCAG 2 cut(s) 4, 63
BspFNI CGCG 5 cut(s) 129, 181, 183, 213, 219
BspLI GGNNCC 4 cut(s) 35, 80, 105, 191
BspTNI GGTCTC 1 cut(s) 224
BsrGI TGTACA 1 cut(s) 84
BssECI CCNNGG 1 cut(s) 161
BssMI GATC 1 cut(s) 121
BstAUI TGTACA 1 cut(s) 84
BstC8I GCNNGC 4 cut(s) 50, 54, 217, 281
BstDEI CTNAG 2 cut(s) 12, 71
BstENI CCTNNNNNAGG 2 cut(s) 86, 98
BstFNI CGCG 5 cut(s) 129, 181, 183, 213, 219
BstHHI GCGC 4 cut(s) 129, 183, 215, 221
BstKTI GATC 1 cut(s) 124
BstMAI GTCTC 2 cut(s) 67, 224
BstMBI GATC 1 cut(s) 121
BstMWI GCNNNNNNNGC 4 cut(s) 117, 126, 154, 216
BstNSI RCATGY 1 cut(s) 283
BstSCI CCNGG 1 cut(s) 161
BstUI CGCG 5 cut(s) 129, 181, 183, 213, 219
BstV1I GCAGC 2 cut(s) 144, 182
BsuRI GGCC 2 cut(s) 92, 210
BtsIMutI CAGTG 1 cut(s) 12
Cac8I GCNNGC 4 cut(s) 50, 54, 217, 281
CaiI CAGNNNCTG 1 cut(s) 11
CfoI GCGC 4 cut(s) 129, 183, 215, 221
Cfr13I GGNCC 3 cut(s) 34, 91, 103
CseI GACGC 1 cut(s) 291
Csp6I GTAC 1 cut(s) 85
CviAII CATG 1 cut(s) 280
CviJI RGCY 7 cut(s) 70, 92, 111, 148, 157, 171, 210
CviKI_1 RGCY 7 cut(s) 70, 92, 111, 148, 157, 171, 210
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 2 cut(s) 12, 71
DpnI GATC 1 cut(s) 123
DpnII GATC 1 cut(s) 121
EaeI YGGCCR 1 cut(s) 208
Eco31I GGTCTC 1 cut(s) 224
Eco47I GGWCC 2 cut(s) 34, 103
EcoNI CCTNNNNNAGG 2 cut(s) 86, 98
EcoO109I RGGNCCY 2 cut(s) 91, 103
Esp3I CGTCTC 1 cut(s) 67
FaeI CATG 1 cut(s) 283
FaiI YATR 3 cut(s) 29, 242, 281
FaqI GGGAC 2 cut(s) 47, 229
FatI CATG 1 cut(s) 279
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 5 cut(s) 46, 57, 158, 196, 211
Fsp4HI GCNGC 5 cut(s) 46, 57, 158, 196, 211
GlaI GCGC 4 cut(s) 128, 182, 214, 220
GluI GCNGC 5 cut(s) 46, 57, 158, 196, 211
GsaI CCCAGC 1 cut(s) 120
HaeIII GGCC 2 cut(s) 92, 210
HapII CCGG 1 cut(s) 163
HgaI GACGC 1 cut(s) 291
HhaI GCGC 4 cut(s) 129, 183, 215, 221
Hin1II CATG 1 cut(s) 283
Hin6I GCGC 4 cut(s) 127, 181, 213, 219
HinP1I GCGC 4 cut(s) 127, 181, 213, 219
HpaII CCGG 1 cut(s) 163
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 1 cut(s) 13
Hpy8I GTNNAC 1 cut(s) 226
HpyAV CCTTC 3 cut(s) 122, 184, 244
HpyCH4V TGCA 2 cut(s) 99, 198
HpyF10VI GCNNNNNNNGC 4 cut(s) 117, 126, 154, 216
HpyF3I CTNAG 2 cut(s) 12, 71
Hsp92II CATG 1 cut(s) 283
HspAI GCGC 4 cut(s) 127, 181, 213, 219
Kzo9I GATC 1 cut(s) 121
LmnI GCTCC 1 cut(s) 176
LpnPI CCDG 5 cut(s) 38, 95, 130, 176, 192
Lsp1109I GCAGC 2 cut(s) 144, 182
LweI GCATC 1 cut(s) 207
MalI GATC 1 cut(s) 123
MboI GATC 1 cut(s) 121
MboII GAAGA 2 cut(s) 193, 307
MnlI CCTC 4 cut(s) 7, 104, 124, 280
MroXI GAANNNNTTC 1 cut(s) 140
MspA1I CMGCKG 1 cut(s) 195
MspI CCGG 1 cut(s) 163
MspR9I CCNGG 1 cut(s) 163
MvnI CGCG 5 cut(s) 129, 181, 183, 213, 219
MwoI GCNNNNNNNGC 4 cut(s) 117, 126, 154, 216
NciI CCSGG 1 cut(s) 163
NdeII GATC 1 cut(s) 121
NlaIII CATG 1 cut(s) 283
NlaIV GGNNCC 4 cut(s) 35, 80, 105, 191
NspI RCATGY 1 cut(s) 283
PaeI GCATGC 1 cut(s) 283
PdmI GAANNNNTTC 1 cut(s) 140
PkrI GCNGC 5 cut(s) 47, 58, 159, 197, 212
PpuMI RGGWCCY 1 cut(s) 103
Psp5II RGGWCCY 1 cut(s) 103
PspFI CCCAGC 1 cut(s) 116
PspN4I GGNNCC 4 cut(s) 35, 80, 105, 191
PspPI GGNCC 3 cut(s) 34, 91, 103
PspPPI RGGWCCY 1 cut(s) 103
PstNI CAGNNNCTG 1 cut(s) 11
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SatI GCNGC 5 cut(s) 46, 57, 158, 196, 211
Sau3AI GATC 1 cut(s) 121
Sau96I GGNCC 3 cut(s) 34, 91, 103
ScrFI CCNGG 1 cut(s) 163
SetI ASST 6 cut(s) 72, 84, 150, 159, 173, 236
SfaNI GCATC 1 cut(s) 207
SinI GGWCC 2 cut(s) 34, 103
SphI GCATGC 1 cut(s) 283
SsiI CCGC 6 cut(s) 43, 46, 56, 139, 193, 211
StyD4I CCNGG 1 cut(s) 161
TatI WGTACW 1 cut(s) 84
TauI GCSGC 3 cut(s) 48, 59, 213
TscAI CASTG 1 cut(s) 12
TseI GCWGC 2 cut(s) 157, 195
TspGWI ACGGA 3 cut(s) 91, 227, 248
TspRI CASTG 1 cut(s) 12
VpaK11BI GGWCC 2 cut(s) 34, 103
XagI CCTNNNNNAGG 2 cut(s) 86, 98
XceI RCATGY 1 cut(s) 283
XmiI GTMKAC 1 cut(s) 225
XmnI GAANNNNTTC 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.