Rh1AG133200

Ribosomal protein S14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0006836
Physical Location & Seq
Reverse (-)
25568756 .. 25572003
3248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG133200.1

Sequence Viewer

Length: 348 bp
ATGTCAGTTTGCCGAGAAAACGCCAACATCCGAGACAACCACCGGCGGTTGCTGGCGGAGAAATTCGAGCTCCGGAGGAACCTGTACAAGGCGCTGTGCAAAGACACGAGCCTCCCCGACGAGTTGCGCGAGGAGAACCGGTACAAGCTCTCCAAGCTTCCTCGAAATAGCTCTTTCACTCGAATCAGGAACCGCTGCATCTTCTCCGGCCGAGGCCGCGCCGTCTACGAGACCTTCCGTATGTCTCGTATCGCTTCCGCGCTCTCGCCTCCAAGGGCATGCTCAACGGCGTCAAGAAAGCGTCATGGTAGTCCGTCCTCCCAAAACGGTTCTGGCAGCGGAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S14

Protein Analysis

115

Amino Acids

13.14

Weight (kDa)

11.02

Isoelectric Point (pI)

63.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S14 PF00253 49 - 84 8.9e-14 Ribosomal protein S14p/S29e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 225
AccII CGCG 3 cut(s) 129, 219, 260
AccIII TCCGGA 1 cut(s) 72
AciI CCGC 6 cut(s) 46, 56, 193, 217, 258, 339
AcoI YGGCCR 1 cut(s) 208
AcsI RAATTY 1 cut(s) 62
AcyI GRCGYC 1 cut(s) 290
AfaI GTAC 2 cut(s) 86, 143
AfiI CCNNNNNNNGG 1 cut(s) 88
AgeI ACCGGT 1 cut(s) 138
AluBI AGCT 4 cut(s) 70, 148, 157, 171
AluI AGCT 4 cut(s) 70, 148, 157, 171
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 3 cut(s) 27, 224, 249
Aor13HI TCCGGA 1 cut(s) 72
AoxI GGCC 2 cut(s) 208, 214
ApeKI GCWGC 2 cut(s) 195, 336
ApoI RAATTY 1 cut(s) 62
AsiGI ACCGGT 1 cut(s) 138
AspLEI GCGC 4 cut(s) 94, 129, 221, 262
BanII GRGCYC 1 cut(s) 72
BauI CACGAG 1 cut(s) 106
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 1 cut(s) 182
BceAI ACGGC 2 cut(s) 206, 303
BcoDI GTCTC 3 cut(s) 27, 224, 249
BfoI RGCGCY 1 cut(s) 95
BisI GCNGC 3 cut(s) 196, 217, 337
BlsI GCNGC 3 cut(s) 197, 218, 338
BmiI GGNNCC 2 cut(s) 80, 191
BmsI GCATC 1 cut(s) 207
BsaHI GRCGYC 1 cut(s) 290
BsaI GGTCTC 1 cut(s) 224
BsaJI CCNNGG 2 cut(s) 211, 272
BsaWI WCCGGW 2 cut(s) 72, 138
BsaXI ACNNNNNCTCC 4 cut(s) 125, 134, 155, 164
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse118I RCCGGY 2 cut(s) 42, 138
BseAI TCCGGA 1 cut(s) 72
BseDI CCNNGG 2 cut(s) 211, 272
BseGI GGATG 1 cut(s) 27
BseLI CCNNNNNNNGG 1 cut(s) 88
BseRI GAGGAG 1 cut(s) 146
BseX3I CGGCCG 1 cut(s) 208
BseXI GCAGC 1 cut(s) 182
Bsh1236I CGCG 3 cut(s) 129, 219, 260
Bsh1285I CGRYCG 1 cut(s) 211
BshFI GGCC 2 cut(s) 210, 216
BshTI ACCGGT 1 cut(s) 138
BsiEI CGRYCG 1 cut(s) 211
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 4 cut(s) 43, 73, 139, 207
BslI CCNNNNNNNGG 1 cut(s) 88
BsmAI GTCTC 3 cut(s) 27, 224, 249
BsnI GGCC 2 cut(s) 210, 216
Bso31I GGTCTC 1 cut(s) 224
Bsp1286I GDGCHC 1 cut(s) 72
Bsp13I TCCGGA 1 cut(s) 72
Bsp1407I TGTACA 1 cut(s) 84
BspACI CCGC 6 cut(s) 46, 56, 193, 217, 258, 339
BspANI GGCC 2 cut(s) 210, 216
BspEI TCCGGA 1 cut(s) 72
BspFNI CGCG 3 cut(s) 129, 219, 260
BspLI GGNNCC 2 cut(s) 80, 191
BspTNI GGTCTC 1 cut(s) 224
BsrFI RCCGGY 2 cut(s) 42, 138
BsrGI TGTACA 1 cut(s) 84
BssAI RCCGGY 2 cut(s) 42, 138
BssECI CCNNGG 2 cut(s) 211, 272
BssNI GRCGYC 1 cut(s) 290
BssSI CACGAG 1 cut(s) 106
BssT1I CCWWGG 1 cut(s) 272
Bst2BI CACGAG 1 cut(s) 106
Bst4CI ACNGT 1 cut(s) 329
BstACI GRCGYC 1 cut(s) 290
BstAUI TGTACA 1 cut(s) 84
BstC8I GCNNGC 2 cut(s) 54, 280
BstENI CCTNNNNNAGG 1 cut(s) 86
BstF5I GGATG 1 cut(s) 27
BstFNI CGCG 3 cut(s) 129, 219, 260
BstH2I RGCGCY 1 cut(s) 95
BstHHI GCGC 4 cut(s) 94, 129, 221, 262
BstMAI GTCTC 3 cut(s) 27, 224, 249
BstMCI CGRYCG 1 cut(s) 211
BstMWI GCNNNNNNNGC 2 cut(s) 154, 216
BstNSI RCATGY 1 cut(s) 282
BstUI CGCG 3 cut(s) 129, 219, 260
BstV1I GCAGC 1 cut(s) 182
BstZI CGGCCG 1 cut(s) 208
BsuRI GGCC 2 cut(s) 210, 216
BtsCI GGATG 1 cut(s) 27
Cac8I GCNNGC 2 cut(s) 54, 280
CfoI GCGC 4 cut(s) 94, 129, 221, 262
Cfr10I RCCGGY 2 cut(s) 42, 138
CseI GACGC 2 cut(s) 279, 290
Csp6I GTAC 2 cut(s) 85, 142
CspAI ACCGGT 1 cut(s) 138
CviAII CATG 2 cut(s) 279, 305
CviJI RGCY 7 cut(s) 70, 111, 148, 157, 171, 210, 216
CviKI_1 RGCY 7 cut(s) 70, 111, 148, 157, 171, 210, 216
CviQI GTAC 2 cut(s) 85, 142
EaeI YGGCCR 1 cut(s) 208
EagI CGGCCG 1 cut(s) 208
EciI GGCGGA 1 cut(s) 71
Ecl136II GAGCTC 1 cut(s) 70
EclXI CGGCCG 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 272
Eco24I GRGCYC 1 cut(s) 72
Eco31I GGTCTC 1 cut(s) 224
Eco52I CGGCCG 1 cut(s) 208
Eco53kI GAGCTC 1 cut(s) 70
EcoICRI GAGCTC 1 cut(s) 70
EcoNI CCTNNNNNAGG 1 cut(s) 86
EcoT14I CCWWGG 1 cut(s) 272
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 272
FaeI CATG 2 cut(s) 282, 308
FaiI YATR 3 cut(s) 242, 280, 306
FatI CATG 2 cut(s) 278, 304
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 3 cut(s) 196, 217, 337
FokI GGATG 1 cut(s) 14
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 3 cut(s) 196, 217, 337
GlaI GCGC 4 cut(s) 93, 128, 220, 261
GluI GCNGC 3 cut(s) 196, 217, 337
HaeII RGCGCY 1 cut(s) 95
HaeIII GGCC 2 cut(s) 210, 216
HapII CCGG 4 cut(s) 43, 73, 139, 207
HgaI GACGC 2 cut(s) 279, 290
HhaI GCGC 4 cut(s) 94, 129, 221, 262
Hin1I GRCGYC 1 cut(s) 290
Hin1II CATG 2 cut(s) 282, 308
Hin6I GCGC 4 cut(s) 92, 127, 219, 260
HinP1I GCGC 4 cut(s) 92, 127, 219, 260
HindIII AAGCTT 1 cut(s) 155
HinfI GANTC 1 cut(s) 183
HpaII CCGG 4 cut(s) 43, 73, 139, 207
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 1 cut(s) 32
Hpy188III TCNNGA 3 cut(s) 73, 187, 294
Hpy8I GTNNAC 1 cut(s) 226
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 1 cut(s) 244
HpyCH4III ACNGT 1 cut(s) 329
HpyCH4V TGCA 2 cut(s) 99, 198
HpyF10VI GCNNNNNNNGC 2 cut(s) 154, 216
Hsp92I GRCGYC 1 cut(s) 290
Hsp92II CATG 2 cut(s) 282, 308
HspAI GCGC 4 cut(s) 92, 127, 219, 260
Kpn2I TCCGGA 1 cut(s) 72
LmnI GCTCC 1 cut(s) 75
LpnPI CCDG 8 cut(s) 38, 56, 86, 95, 152, 172, 220, 318
Lsp1109I GCAGC 1 cut(s) 182
LweI GCATC 1 cut(s) 207
MboII GAAGA 1 cut(s) 193
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 1 cut(s) 62
MnlI CCTC 7 cut(s) 69, 122, 124, 171, 206, 279, 328
MroI TCCGGA 1 cut(s) 72
MspA1I CMGCKG 2 cut(s) 195, 339
MspI CCGG 4 cut(s) 43, 73, 139, 207
MvnI CGCG 3 cut(s) 129, 219, 260
MwoI GCNNNNNNNGC 2 cut(s) 154, 216
NlaIII CATG 2 cut(s) 282, 308
NlaIV GGNNCC 2 cut(s) 80, 191
NmeAIII GCCGAG 2 cut(s) 38, 236
NspI RCATGY 1 cut(s) 282
PaeI GCATGC 1 cut(s) 282
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PfeI GAWTC 1 cut(s) 183
PinAI ACCGGT 1 cut(s) 138
PkrI GCNGC 3 cut(s) 197, 218, 338
Psp124BI GAGCTC 1 cut(s) 72
PspN4I GGNNCC 2 cut(s) 80, 191
RsaI GTAC 2 cut(s) 86, 143
RsaNI GTAC 2 cut(s) 85, 142
SacI GAGCTC 1 cut(s) 72
SatI GCNGC 3 cut(s) 196, 217, 337
SduI GDGCHC 1 cut(s) 72
SetI ASST 6 cut(s) 72, 84, 150, 159, 173, 236
SfaNI GCATC 1 cut(s) 207
SgrAI CRCCGGYG 1 cut(s) 42
SphI GCATGC 1 cut(s) 282
Sse9I AATT 1 cut(s) 62
SsiI CCGC 6 cut(s) 46, 56, 193, 217, 258, 339
SstI GAGCTC 1 cut(s) 72
StyI CCWWGG 1 cut(s) 272
TaaI ACNGT 1 cut(s) 329
TaqI TCGA 3 cut(s) 66, 163, 181
TasI AATT 1 cut(s) 62
TatI WGTACW 1 cut(s) 84
TauI GCSGC 1 cut(s) 219
TfiI GAWTC 1 cut(s) 183
TseI GCWGC 2 cut(s) 195, 336
TspGWI ACGGA 2 cut(s) 227, 303
XagI CCTNNNNNAGG 1 cut(s) 86
XapI RAATTY 1 cut(s) 62
XceI RCATGY 1 cut(s) 282
XcmI CCANNNNNNNNNTGG 1 cut(s) 329
XmiI GTMKAC 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.