Rroxscaffold_1G00023870

Ribosomal protein S14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006836
Physical Location & Seq
Forward (+)
29508388 .. 29508699
312 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_1G00023870.1

Sequence Viewer

Length: 312 bp
ATGTCAGTGTGTAGAGAAAACGCGAACATCAGAGACAACCACCGCCGTCTGCTGGCGGAGAAATTCGAGCTGCGGCGGAACCTGTACAAGGCGCTGTGCAAGGACACGAGCCTCCCCGACGAGTTGCGCGAGGAGAATCGGTACAAGCTCTCGAAGCTTCCTCGGAACAGCTCCTTCACCAGGATCAGGAACCGCTGCATCTTCTCCGGCCGAGGCCGCGCCGTCTACGAGACCTTCCGCATGTCGCGTATCGTCTTCCGCGCTCTCGCCTCCAAGGGCATGCTCAACGGCGTCAAGAAGGCTTCGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S14

Protein Analysis

103

Amino Acids

12.21

Weight (kDa)

10.9

Isoelectric Point (pI)

42.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S14 PF00253 49 - 102 5.9e-24 Ribosomal protein S14p/S29e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 225
AccII CGCG 5 cut(s) 23, 129, 219, 247, 261
AciI CCGC 8 cut(s) 43, 56, 73, 76, 193, 217, 238, 259
AclWI GGATC 1 cut(s) 191
AcoI YGGCCR 1 cut(s) 208
AcsI RAATTY 1 cut(s) 62
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 2 cut(s) 86, 143
AfiI CCNNNNNNNGG 4 cut(s) 52, 88, 180, 186
AjnI CCWGG 1 cut(s) 179
AluBI AGCT 4 cut(s) 70, 148, 157, 171
AluI AGCT 4 cut(s) 70, 148, 157, 171
Alw26I GTCTC 2 cut(s) 27, 224
AlwI GGATC 1 cut(s) 191
AoxI GGCC 2 cut(s) 208, 214
ApeKI GCWGC 2 cut(s) 70, 195
ApoI RAATTY 1 cut(s) 62
AspLEI GCGC 4 cut(s) 94, 129, 221, 263
AsuHPI GGTGA 1 cut(s) 169
BauI CACGAG 1 cut(s) 106
BbsI GAAGAC 1 cut(s) 247
BbvI GCAGC 2 cut(s) 57, 182
BceAI ACGGC 3 cut(s) 30, 206, 304
BciT130I CCWGG 1 cut(s) 181
BcoDI GTCTC 2 cut(s) 27, 224
BfoI RGCGCY 1 cut(s) 95
BisI GCNGC 4 cut(s) 71, 74, 196, 217
BlsI GCNGC 4 cut(s) 72, 75, 197, 218
Bme1390I CCNGG 1 cut(s) 181
BmiI GGNNCC 2 cut(s) 80, 191
BmrFI CCNGG 1 cut(s) 181
BmsI GCATC 1 cut(s) 207
BpiI GAAGAC 1 cut(s) 247
BsaHI GRCGYC 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 224
BsaJI CCNNGG 3 cut(s) 161, 211, 273
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 88, 180, 186
BseBI CCWGG 1 cut(s) 181
BseDI CCNNGG 3 cut(s) 161, 211, 273
BseLI CCNNNNNNNGG 4 cut(s) 52, 88, 180, 186
BseRI GAGGAG 1 cut(s) 146
BseX3I CGGCCG 1 cut(s) 208
BseXI GCAGC 2 cut(s) 57, 182
Bsh1236I CGCG 5 cut(s) 23, 129, 219, 247, 261
Bsh1285I CGRYCG 1 cut(s) 211
BshFI GGCC 2 cut(s) 210, 216
BsiEI CGRYCG 1 cut(s) 211
BsiSI CCGG 1 cut(s) 207
BslI CCNNNNNNNGG 4 cut(s) 52, 88, 180, 186
BsmAI GTCTC 2 cut(s) 27, 224
BsnI GGCC 2 cut(s) 210, 216
Bso31I GGTCTC 1 cut(s) 224
Bsp1407I TGTACA 1 cut(s) 84
Bsp143I GATC 1 cut(s) 183
BspACI CCGC 8 cut(s) 43, 56, 73, 76, 193, 217, 238, 259
BspANI GGCC 2 cut(s) 210, 216
BspFNI CGCG 5 cut(s) 23, 129, 219, 247, 261
BspLI GGNNCC 2 cut(s) 80, 191
BspPI GGATC 1 cut(s) 191
BspTNI GGTCTC 1 cut(s) 224
BsrGI TGTACA 1 cut(s) 84
BssECI CCNNGG 3 cut(s) 161, 211, 273
BssMI GATC 1 cut(s) 183
BssNI GRCGYC 1 cut(s) 291
BssSI CACGAG 1 cut(s) 106
BssT1I CCWWGG 1 cut(s) 273
Bst2BI CACGAG 1 cut(s) 106
Bst2UI CCWGG 1 cut(s) 181
BstACI GRCGYC 1 cut(s) 291
BstAUI TGTACA 1 cut(s) 84
BstC8I GCNNGC 2 cut(s) 54, 281
BstENI CCTNNNNNAGG 2 cut(s) 86, 178
BstFNI CGCG 5 cut(s) 23, 129, 219, 247, 261
BstH2I RGCGCY 1 cut(s) 95
BstHHI GCGC 4 cut(s) 94, 129, 221, 263
BstKTI GATC 1 cut(s) 186
BstMAI GTCTC 2 cut(s) 27, 224
BstMBI GATC 1 cut(s) 183
BstMCI CGRYCG 1 cut(s) 211
BstMWI GCNNNNNNNGC 2 cut(s) 154, 216
BstNI CCWGG 1 cut(s) 181
BstNSI RCATGY 2 cut(s) 244, 283
BstSCI CCNGG 1 cut(s) 179
BstUI CGCG 5 cut(s) 23, 129, 219, 247, 261
BstV1I GCAGC 2 cut(s) 57, 182
BstV2I GAAGAC 1 cut(s) 247
BstZI CGGCCG 1 cut(s) 208
BsuRI GGCC 2 cut(s) 210, 216
BtsIMutI CAGTG 1 cut(s) 12
Cac8I GCNNGC 2 cut(s) 54, 281
CfoI GCGC 4 cut(s) 94, 129, 221, 263
CseI GACGC 1 cut(s) 280
Csp6I GTAC 2 cut(s) 85, 142
CviAII CATG 2 cut(s) 241, 280
CviJI RGCY 8 cut(s) 70, 111, 148, 157, 171, 210, 216, 302
CviKI_1 RGCY 8 cut(s) 70, 111, 148, 157, 171, 210, 216, 302
CviQI GTAC 2 cut(s) 85, 142
DpnI GATC 1 cut(s) 185
DpnII GATC 1 cut(s) 183
EaeI YGGCCR 1 cut(s) 208
EagI CGGCCG 1 cut(s) 208
EciI GGCGGA 2 cut(s) 71, 91
EclXI CGGCCG 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 273
Eco31I GGTCTC 1 cut(s) 224
Eco52I CGGCCG 1 cut(s) 208
EcoNI CCTNNNNNAGG 2 cut(s) 86, 178
EcoRII CCWGG 1 cut(s) 179
EcoT14I CCWWGG 1 cut(s) 273
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 2 cut(s) 244, 283
FaiI YATR 2 cut(s) 242, 281
FatI CATG 2 cut(s) 240, 279
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 4 cut(s) 71, 74, 196, 217
Fsp4HI GCNGC 4 cut(s) 71, 74, 196, 217
GlaI GCGC 4 cut(s) 93, 128, 220, 262
GluI GCNGC 4 cut(s) 71, 74, 196, 217
HaeII RGCGCY 1 cut(s) 95
HaeIII GGCC 2 cut(s) 210, 216
HapII CCGG 1 cut(s) 207
HgaI GACGC 1 cut(s) 280
HhaI GCGC 4 cut(s) 94, 129, 221, 263
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 2 cut(s) 244, 283
Hin6I GCGC 4 cut(s) 92, 127, 219, 261
HinP1I GCGC 4 cut(s) 92, 127, 219, 261
HindIII AAGCTT 1 cut(s) 155
HinfI GANTC 1 cut(s) 136
HpaII CCGG 1 cut(s) 207
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 2 cut(s) 32, 165
Hpy188III TCNNGA 3 cut(s) 151, 187, 295
Hpy8I GTNNAC 1 cut(s) 226
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 3 cut(s) 184, 244, 292
HpyCH4V TGCA 2 cut(s) 99, 198
HpyF10VI GCNNNNNNNGC 2 cut(s) 154, 216
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 2 cut(s) 244, 283
HspAI GCGC 4 cut(s) 92, 127, 219, 261
Kzo9I GATC 1 cut(s) 183
LmnI GCTCC 1 cut(s) 176
LpnPI CCDG 6 cut(s) 38, 95, 166, 172, 193, 220
Lsp1109I GCAGC 2 cut(s) 57, 182
LweI GCATC 1 cut(s) 207
MalI GATC 1 cut(s) 185
MboI GATC 1 cut(s) 183
MboII GAAGA 2 cut(s) 193, 247
MluCI AATT 1 cut(s) 62
MnlI CCTC 5 cut(s) 122, 124, 171, 206, 280
MspA1I CMGCKG 1 cut(s) 195
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 181
MvaI CCWGG 1 cut(s) 181
MvnI CGCG 5 cut(s) 23, 129, 219, 247, 261
MwoI GCNNNNNNNGC 2 cut(s) 154, 216
NdeII GATC 1 cut(s) 183
NlaIII CATG 2 cut(s) 244, 283
NlaIV GGNNCC 2 cut(s) 80, 191
NmeAIII GCCGAG 1 cut(s) 236
NspI RCATGY 2 cut(s) 244, 283
PaeI GCATGC 1 cut(s) 283
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PfeI GAWTC 1 cut(s) 136
PkrI GCNGC 4 cut(s) 72, 75, 197, 218
Psp6I CCWGG 1 cut(s) 179
PspGI CCWGG 1 cut(s) 179
PspN4I GGNNCC 2 cut(s) 80, 191
RsaI GTAC 2 cut(s) 86, 143
RsaNI GTAC 2 cut(s) 85, 142
SatI GCNGC 4 cut(s) 71, 74, 196, 217
Sau3AI GATC 1 cut(s) 183
ScrFI CCNGG 1 cut(s) 181
SetI ASST 6 cut(s) 72, 84, 150, 159, 173, 236
SfaNI GCATC 1 cut(s) 207
SphI GCATGC 1 cut(s) 283
Sse9I AATT 1 cut(s) 62
SsiI CCGC 8 cut(s) 43, 56, 73, 76, 193, 217, 238, 259
StyD4I CCNGG 1 cut(s) 179
StyI CCWWGG 1 cut(s) 273
TaqI TCGA 2 cut(s) 66, 152
TasI AATT 1 cut(s) 62
TatI WGTACW 1 cut(s) 84
TauI GCSGC 2 cut(s) 76, 219
TfiI GAWTC 1 cut(s) 136
TscAI CASTG 1 cut(s) 12
TseI GCWGC 2 cut(s) 70, 195
TspRI CASTG 1 cut(s) 12
XagI CCTNNNNNAGG 2 cut(s) 86, 178
XapI RAATTY 1 cut(s) 62
XceI RCATGY 2 cut(s) 244, 283
XmiI GTMKAC 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.