RLG00000029548

Ribosomal protein S14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0006836
Physical Location & Seq
Forward (+)
40212323 .. 40212976
654 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029548

Sequence Viewer

Length: 438 bp
ATGTACAAAACTTCGAACTGGCCCATTCATCGTGTACCATCCTTAGTCCATCCAGAGGCGACCGGCTACGGAGATCACTTCAAATCTCCTGGCGGCGATGGAATGAGTGGCTGCGTAAAACCCAAACTGTCAGTTTGCCGAGAAAACGCCAACATCCAAGACAACCACCGGCGGTTGCTGGCAGAGAAATTCGAGCTCCGGCGGAACCTGTACAAGGCGCTGTGCAAAGACACGGGCCTCCCCGACGAGTTGCGCGAGGAGAACCGGTACAAGCTCTCAAGGCTTCCTCGAAACAGCTCCTTAACACGCATCAGGAACCGCTGCATCTTCTCCGGCCGAGGCCGCGCCGTCTACGAGACCTTCCGTATGTCTCGTATCGTCTTCCGCGCTCTCGCCTCCAAGGCCATGCTCAACGGCGTCAAGAAAGCGTCATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S14

Protein Analysis

146

Amino Acids

16.7

Weight (kDa)

10.32

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S14 PF00253 91 - 144 2.5e-22 Ribosomal protein S14p/S29e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 351
AccII CGCG 3 cut(s) 255, 345, 387
AciI CCGC 6 cut(s) 93, 172, 202, 319, 343, 385
AcoI YGGCCR 1 cut(s) 334
AcsI RAATTY 1 cut(s) 188
AcyI GRCGYC 1 cut(s) 417
AfaI GTAC 4 cut(s) 5, 36, 212, 269
AfiI CCNNNNNNNGG 2 cut(s) 55, 214
AgeI ACCGGT 1 cut(s) 264
AgsI TTSAA 1 cut(s) 82
AjnI CCWGG 1 cut(s) 88
AluBI AGCT 3 cut(s) 196, 274, 297
AluI AGCT 3 cut(s) 196, 274, 297
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 2 cut(s) 350, 375
AoxI GGCC 5 cut(s) 20, 235, 334, 340, 402
ApeKI GCWGC 2 cut(s) 111, 321
ApoI RAATTY 1 cut(s) 188
AsiGI ACCGGT 1 cut(s) 264
AspLEI GCGC 4 cut(s) 220, 255, 347, 389
AspS9I GGNCC 2 cut(s) 21, 235
AsuII TTCGAA 1 cut(s) 14
BanII GRGCYC 1 cut(s) 198
BbsI GAAGAC 1 cut(s) 373
Bbv12I GWGCWC 1 cut(s) 198
BbvI GCAGC 2 cut(s) 98, 308
BccI CCATC 3 cut(s) 46, 57, 92
BceAI ACGGC 2 cut(s) 332, 430
BciT130I CCWGG 1 cut(s) 90
BcoDI GTCTC 2 cut(s) 350, 375
BfoI RGCGCY 1 cut(s) 221
BglI GCCNNNNNGGC 1 cut(s) 401
BisI GCNGC 4 cut(s) 94, 112, 322, 343
BlsI GCNGC 4 cut(s) 95, 113, 323, 344
Bme1390I CCNGG 1 cut(s) 90
BmgT120I GGNCC 2 cut(s) 21, 235
BmiI GGNNCC 2 cut(s) 206, 317
BmrFI CCNGG 1 cut(s) 90
BmsI GCATC 2 cut(s) 318, 333
BpiI GAAGAC 1 cut(s) 373
Bpu14I TTCGAA 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 262
BsaHI GRCGYC 1 cut(s) 417
BsaI GGTCTC 1 cut(s) 350
BsaJI CCNNGG 2 cut(s) 337, 399
BsaWI WCCGGW 1 cut(s) 264
BsaXI ACNNNNNCTCC 2 cut(s) 251, 281
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 214
Bse118I RCCGGY 3 cut(s) 62, 168, 264
Bse1I ACTGG 1 cut(s) 23
BseBI CCWGG 1 cut(s) 90
BseDI CCNNGG 2 cut(s) 337, 399
BseGI GGATG 3 cut(s) 38, 49, 153
BseLI CCNNNNNNNGG 2 cut(s) 55, 214
BseNI ACTGG 1 cut(s) 23
BseRI GAGGAG 1 cut(s) 272
BseX3I CGGCCG 1 cut(s) 334
BseXI GCAGC 2 cut(s) 98, 308
Bsh1236I CGCG 3 cut(s) 255, 345, 387
Bsh1285I CGRYCG 2 cut(s) 63, 337
BshFI GGCC 5 cut(s) 22, 237, 336, 342, 404
BshTI ACCGGT 1 cut(s) 264
BsiEI CGRYCG 2 cut(s) 63, 337
BsiHKAI GWGCWC 1 cut(s) 198
BsiSI CCGG 5 cut(s) 63, 169, 199, 265, 333
BslI CCNNNNNNNGG 2 cut(s) 55, 214
BsmAI GTCTC 2 cut(s) 350, 375
BsnI GGCC 5 cut(s) 22, 237, 336, 342, 404
Bso31I GGTCTC 1 cut(s) 350
Bsp119I TTCGAA 1 cut(s) 14
Bsp1286I GDGCHC 1 cut(s) 198
Bsp1407I TGTACA 2 cut(s) 3, 210
Bsp143I GATC 1 cut(s) 73
BspACI CCGC 6 cut(s) 93, 172, 202, 319, 343, 385
BspANI GGCC 5 cut(s) 22, 237, 336, 342, 404
BspFNI CGCG 3 cut(s) 255, 345, 387
BspLI GGNNCC 2 cut(s) 206, 317
BspT104I TTCGAA 1 cut(s) 14
BspTNI GGTCTC 1 cut(s) 350
BsrFI RCCGGY 3 cut(s) 62, 168, 264
BsrGI TGTACA 2 cut(s) 3, 210
BsrI ACTGG 1 cut(s) 23
BssAI RCCGGY 3 cut(s) 62, 168, 264
BssECI CCNNGG 2 cut(s) 337, 399
BssMI GATC 1 cut(s) 73
BssNI GRCGYC 1 cut(s) 417
BssT1I CCWWGG 1 cut(s) 399
Bst2UI CCWGG 1 cut(s) 90
Bst4CI ACNGT 1 cut(s) 129
BstACI GRCGYC 1 cut(s) 417
BstAUI TGTACA 2 cut(s) 3, 210
BstBI TTCGAA 1 cut(s) 14
BstC8I GCNNGC 1 cut(s) 180
BstDEI CTNAG 1 cut(s) 43
BstENI CCTNNNNNAGG 1 cut(s) 212
BstF5I GGATG 3 cut(s) 38, 49, 153
BstFNI CGCG 3 cut(s) 255, 345, 387
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 4 cut(s) 220, 255, 347, 389
BstKTI GATC 1 cut(s) 76
BstMAI GTCTC 2 cut(s) 350, 375
BstMBI GATC 1 cut(s) 73
BstMCI CGRYCG 2 cut(s) 63, 337
BstMWI GCNNNNNNNGC 3 cut(s) 280, 342, 401
BstNI CCWGG 1 cut(s) 90
BstSCI CCNGG 1 cut(s) 88
BstUI CGCG 3 cut(s) 255, 345, 387
BstV1I GCAGC 2 cut(s) 98, 308
BstV2I GAAGAC 1 cut(s) 373
BstZI CGGCCG 1 cut(s) 334
BsuRI GGCC 5 cut(s) 22, 237, 336, 342, 404
BtgZI GCGATG 1 cut(s) 111
BtsCI GGATG 3 cut(s) 38, 49, 153
Cac8I GCNNGC 1 cut(s) 180
CfoI GCGC 4 cut(s) 220, 255, 347, 389
Cfr10I RCCGGY 3 cut(s) 62, 168, 264
Cfr13I GGNCC 2 cut(s) 21, 235
CseI GACGC 2 cut(s) 406, 417
Csp6I GTAC 4 cut(s) 4, 35, 211, 268
CspAI ACCGGT 1 cut(s) 264
CviAII CATG 2 cut(s) 406, 432
CviQI GTAC 4 cut(s) 4, 35, 211, 268
DdeI CTNAG 1 cut(s) 43
DpnI GATC 1 cut(s) 75
DpnII GATC 1 cut(s) 73
EaeI YGGCCR 1 cut(s) 334
EagI CGGCCG 1 cut(s) 334
EciI GGCGGA 1 cut(s) 217
Ecl136II GAGCTC 1 cut(s) 196
EclXI CGGCCG 1 cut(s) 334
Eco130I CCWWGG 1 cut(s) 399
Eco24I GRGCYC 1 cut(s) 198
Eco31I GGTCTC 1 cut(s) 350
Eco52I CGGCCG 1 cut(s) 334
Eco53kI GAGCTC 1 cut(s) 196
EcoICRI GAGCTC 1 cut(s) 196
EcoNI CCTNNNNNAGG 1 cut(s) 212
EcoRII CCWGG 1 cut(s) 88
EcoT14I CCWWGG 1 cut(s) 399
EcoT38I GRGCYC 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 399
FaeI CATG 2 cut(s) 409, 435
FaiI YATR 3 cut(s) 368, 407, 433
FatI CATG 2 cut(s) 405, 431
FblI GTMKAC 1 cut(s) 351
Fnu4HI GCNGC 4 cut(s) 94, 112, 322, 343
FokI GGATG 3 cut(s) 25, 36, 140
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 4 cut(s) 94, 112, 322, 343
GlaI GCGC 4 cut(s) 219, 254, 346, 388
GluI GCNGC 4 cut(s) 94, 112, 322, 343
HaeII RGCGCY 1 cut(s) 221
HaeIII GGCC 5 cut(s) 22, 237, 336, 342, 404
HapII CCGG 5 cut(s) 63, 169, 199, 265, 333
HgaI GACGC 2 cut(s) 406, 417
HhaI GCGC 4 cut(s) 220, 255, 347, 389
Hin1I GRCGYC 1 cut(s) 417
Hin1II CATG 2 cut(s) 409, 435
Hin6I GCGC 4 cut(s) 218, 253, 345, 387
HinP1I GCGC 4 cut(s) 218, 253, 345, 387
HpaII CCGG 5 cut(s) 63, 169, 199, 265, 333
Hpy166II GTNNAC 2 cut(s) 35, 352
Hpy188III TCNNGA 3 cut(s) 53, 313, 421
Hpy8I GTNNAC 2 cut(s) 35, 352
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 1 cut(s) 370
HpyCH4III ACNGT 1 cut(s) 129
HpyCH4V TGCA 2 cut(s) 225, 324
HpyF10VI GCNNNNNNNGC 3 cut(s) 280, 342, 401
HpyF3I CTNAG 1 cut(s) 43
Hsp92I GRCGYC 1 cut(s) 417
Hsp92II CATG 2 cut(s) 409, 435
HspAI GCGC 4 cut(s) 218, 253, 345, 387
Kzo9I GATC 1 cut(s) 73
LmnI GCTCC 2 cut(s) 201, 302
Lsp1109I GCAGC 2 cut(s) 98, 308
LweI GCATC 2 cut(s) 318, 333
MalI GATC 1 cut(s) 75
MboI GATC 1 cut(s) 73
MboII GAAGA 2 cut(s) 319, 373
MhlI GDGCHC 1 cut(s) 198
MluCI AATT 1 cut(s) 188
MnlI CCTC 6 cut(s) 49, 248, 250, 297, 332, 406
MseI TTAA 1 cut(s) 302
MspA1I CMGCKG 1 cut(s) 321
MspI CCGG 5 cut(s) 63, 169, 199, 265, 333
MspR9I CCNGG 1 cut(s) 90
MvaI CCWGG 1 cut(s) 90
MvnI CGCG 3 cut(s) 255, 345, 387
MwoI GCNNNNNNNGC 3 cut(s) 280, 342, 401
NdeII GATC 1 cut(s) 73
NlaIII CATG 2 cut(s) 409, 435
NlaIV GGNNCC 2 cut(s) 206, 317
NmeAIII GCCGAG 2 cut(s) 164, 362
NspV TTCGAA 1 cut(s) 14
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PinAI ACCGGT 1 cut(s) 264
PkrI GCNGC 4 cut(s) 95, 113, 323, 344
Psp124BI GAGCTC 1 cut(s) 198
Psp6I CCWGG 1 cut(s) 88
PspGI CCWGG 1 cut(s) 88
PspN4I GGNNCC 2 cut(s) 206, 317
PspPI GGNCC 2 cut(s) 21, 235
RsaI GTAC 4 cut(s) 5, 36, 212, 269
RsaNI GTAC 4 cut(s) 4, 35, 211, 268
SacI GAGCTC 1 cut(s) 198
SaqAI TTAA 1 cut(s) 302
SatI GCNGC 4 cut(s) 94, 112, 322, 343
Sau3AI GATC 1 cut(s) 73
Sau96I GGNCC 2 cut(s) 21, 235
ScrFI CCNGG 1 cut(s) 90
SduI GDGCHC 1 cut(s) 198
SetI ASST 5 cut(s) 198, 210, 276, 299, 362
SfaNI GCATC 2 cut(s) 318, 333
SfuI TTCGAA 1 cut(s) 14
SgrAI CRCCGGYG 1 cut(s) 168
SmlI CTYRAG 1 cut(s) 277
SmoI CTYRAG 1 cut(s) 277
Sse9I AATT 1 cut(s) 188
SsiI CCGC 6 cut(s) 93, 172, 202, 319, 343, 385
SstI GAGCTC 1 cut(s) 198
StyD4I CCNGG 1 cut(s) 88
StyI CCWWGG 1 cut(s) 399
TaaI ACNGT 1 cut(s) 129
TaqI TCGA 3 cut(s) 14, 192, 289
TasI AATT 1 cut(s) 188
TatI WGTACW 2 cut(s) 3, 210
TauI GCSGC 2 cut(s) 96, 345
Tru1I TTAA 1 cut(s) 302
Tru9I TTAA 1 cut(s) 302
TseI GCWGC 2 cut(s) 111, 321
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 84, 353
XagI CCTNNNNNAGG 1 cut(s) 212
XapI RAATTY 1 cut(s) 188
XmiI GTMKAC 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.