FvH4_3g34300

Nucleolar protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009144
Physical Location & Seq
Forward (+)
29809504 .. 29810673
1170 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g34300.t1

Sequence Viewer

Length: 753 bp
ATGAAGACGGTCCAAAGCCATGTCCTCTCCTCCGACCCAATCTCCCTCTCCAACGCCACCTCCTTCCTCTCCGCCTTCAACAAGTCCGTCTGCGGAGGCTTTGGCGCCGCCTCGCTCGCCGCGTCCCACGAGACCTGCTGGTTCGTCCGACGCGCCCTGGCCTCCTTCGAGGAGCTGGAGCAGCTCCACCACAAGCTCAAGGGGCCCAAGTCCAATCGCAATCGGAGGTCGGAGTCTCAAAACGACGACGTAGAGACCGCCGTTGGGGACAATCCGGCGCAGAGTTTCGTGTCGAGCCGGGACGTGAGCTCCATGCCCACAAGCCACCGTCATGATGACGTCATCGATCGGAAAGTCGCGGTTGGAGAAGAGAGTAAGGGCGGGAGTCGTAATTTGAAGGATGGAGGTGAAATTGCGGAGATTGATGATAAGAAGAAGAAGGAAAAGGAGGTGAAGGGTGCTATTGGTAATATTGGCGAAAACGGAGGCCGAATTGAGGAGGGAGATGGTGAGAGTAAGATGCGAAATGGAGAGGTAAAGAGGGAGAAGAAGAAGAAGAAAAAGAAGAAGGGGGACAATGTGGATGAAAGTGGGGGTGGAATTAAGGAGGAGGAGGATGGGGAAGGTAAAATGGGGAGCGAAGAGGCGAAGAAGGAAGAGAAGAAGGAGAGGAAGAGTGAGGTGTTGGAAAATGACGAGGTTGATGCTTTGGAGGAACAACAGGAGAAGAGAAAGAAGAGGAGGAAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

251

Amino Acids

27.58

Weight (kDa)

7.04

Isoelectric Point (pI)

57.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 342
Acc36I ACCTGC 1 cut(s) 143
AccB1I GGYRCC 1 cut(s) 104
AccII CGCG 3 cut(s) 122, 153, 359
AciI CCGC 8 cut(s) 72, 93, 108, 120, 258, 359, 381, 416
AcyI GRCGYC 2 cut(s) 105, 339
AfiI CCNNNNNNNGG 2 cut(s) 264, 496
AgsI TTSAA 2 cut(s) 79, 397
AjiI CACGTC 1 cut(s) 304
AjnI CCWGG 1 cut(s) 156
AluBI AGCT 5 cut(s) 175, 184, 196, 309, 750
AluI AGCT 5 cut(s) 175, 184, 196, 309, 750
Alw21I GWGCWC 1 cut(s) 311
Alw26I GTCTC 3 cut(s) 125, 240, 248
AoxI GGCC 3 cut(s) 159, 203, 487
ApaI GGGCCC 1 cut(s) 207
ApeKI GCWGC 1 cut(s) 181
AspLEI GCGC 3 cut(s) 107, 155, 280
AspS9I GGNCC 3 cut(s) 10, 203, 204
AsuC2I CCSGG 1 cut(s) 299
AsuHPI GGTGA 3 cut(s) 419, 463, 521
AvaII GGWCC 1 cut(s) 10
BaeGI GKGCMC 1 cut(s) 207
BanI GGYRCC 1 cut(s) 104
BanII GRGCYC 2 cut(s) 207, 311
BauI CACGAG 1 cut(s) 128
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 311
BbvI GCAGC 1 cut(s) 193
BccI CCATC 3 cut(s) 395, 500, 611
BceAI ACGGC 1 cut(s) 245
BcgI CGANNNNNNTGC 2 cut(s) 686, 720
BciT130I CCWGG 1 cut(s) 158
BcnI CCSGG 1 cut(s) 299
BcoDI GTCTC 3 cut(s) 125, 240, 248
BfoI RGCGCY 1 cut(s) 108
BfuAI ACCTGC 1 cut(s) 143
BisI GCNGC 3 cut(s) 108, 120, 182
BlsI GCNGC 3 cut(s) 109, 121, 183
Bme1390I CCNGG 2 cut(s) 158, 299
Bme18I GGWCC 1 cut(s) 10
BmgBI CACGTC 1 cut(s) 304
BmgT120I GGNCC 3 cut(s) 10, 203, 204
BmiI GGNNCC 3 cut(s) 106, 204, 205
BmrFI CCNGG 2 cut(s) 158, 299
BmsI GCATC 2 cut(s) 510, 694
BpiI GAAGAC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 197
BpuEI CTTGAG 1 cut(s) 182
BpuMI CCSGG 1 cut(s) 299
Bsa29I ATCGAT 1 cut(s) 345
BsaHI GRCGYC 2 cut(s) 105, 339
BsaI GGTCTC 2 cut(s) 125, 248
BsaJI CCNNGG 1 cut(s) 156
BsaXI ACNNNNNCTCC 6 cut(s) 26, 44, 56, 74, 293, 323
Bsc4I CCNNNNNNNGG 2 cut(s) 264, 496
BseBI CCWGG 1 cut(s) 158
BseCI ATCGAT 1 cut(s) 345
BseDI CCNNGG 1 cut(s) 156
BseGI GGATG 3 cut(s) 406, 589, 622
BseLI CCNNNNNNNGG 2 cut(s) 264, 496
BseRI GAGGAG 5 cut(s) 19, 185, 512, 623, 626
BseSI GKGCMC 1 cut(s) 207
BseXI GCAGC 1 cut(s) 193
Bsh1236I CGCG 3 cut(s) 122, 153, 359
Bsh1285I CGRYCG 1 cut(s) 349
BshFI GGCC 3 cut(s) 161, 205, 489
BshNI GGYRCC 1 cut(s) 104
BshVI ATCGAT 1 cut(s) 345
BsiEI CGRYCG 1 cut(s) 349
BsiHKAI GWGCWC 1 cut(s) 311
BsiSI CCGG 2 cut(s) 275, 298
BslFI GGGAC 4 cut(s) 109, 281, 314, 587
BslI CCNNNNNNNGG 2 cut(s) 264, 496
BsmAI GTCTC 3 cut(s) 125, 240, 248
BsmFI GGGAC 4 cut(s) 109, 281, 314, 587
BsnI GGCC 3 cut(s) 161, 205, 489
Bso31I GGTCTC 2 cut(s) 125, 248
Bsp120I GGGCCC 1 cut(s) 203
Bsp1286I GDGCHC 2 cut(s) 207, 311
Bsp143I GATC 1 cut(s) 346
BspACI CCGC 8 cut(s) 72, 93, 108, 120, 258, 359, 381, 416
BspANI GGCC 3 cut(s) 161, 205, 489
BspDI ATCGAT 1 cut(s) 345
BspFNI CGCG 3 cut(s) 122, 153, 359
BspHI TCATGA 1 cut(s) 331
BspLI GGNNCC 3 cut(s) 106, 204, 205
BspMI ACCTGC 1 cut(s) 143
BspT107I GGYRCC 1 cut(s) 104
BspTNI GGTCTC 2 cut(s) 125, 248
BssECI CCNNGG 1 cut(s) 156
BssMI GATC 1 cut(s) 346
BssNI GRCGYC 2 cut(s) 105, 339
BssSI CACGAG 1 cut(s) 128
Bst2BI CACGAG 1 cut(s) 128
Bst2UI CCWGG 1 cut(s) 158
Bst4CI ACNGT 2 cut(s) 10, 329
Bst6I CTCTTC 6 cut(s) 363, 636, 651, 668, 722, 731
BstACI GRCGYC 2 cut(s) 105, 339
BstC8I GCNNGC 1 cut(s) 117
BstF5I GGATG 3 cut(s) 406, 589, 622
BstFNI CGCG 3 cut(s) 122, 153, 359
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 3 cut(s) 107, 155, 280
BstKTI GATC 1 cut(s) 349
BstMAI GTCTC 3 cut(s) 125, 240, 248
BstMBI GATC 1 cut(s) 346
BstMCI CGRYCG 1 cut(s) 349
BstMWI GCNNNNNNNGC 3 cut(s) 116, 181, 202
BstNI CCWGG 1 cut(s) 158
BstSCI CCNGG 2 cut(s) 156, 297
BstSLI GKGCMC 1 cut(s) 207
BstUI CGCG 3 cut(s) 122, 153, 359
BstV1I GCAGC 1 cut(s) 193
BstV2I GAAGAC 1 cut(s) 11
Bsu15I ATCGAT 1 cut(s) 345
BsuRI GGCC 3 cut(s) 161, 205, 489
BsuTUI ATCGAT 1 cut(s) 345
BtrI CACGTC 1 cut(s) 304
BtsCI GGATG 3 cut(s) 406, 589, 622
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 1 cut(s) 117
CciI TCATGA 1 cut(s) 331
CfoI GCGC 3 cut(s) 107, 155, 280
Cfr13I GGNCC 3 cut(s) 10, 203, 204
ClaI ATCGAT 1 cut(s) 345
CseI GACGC 2 cut(s) 111, 159
CviAII CATG 3 cut(s) 20, 313, 332
DinI GGCGCC 1 cut(s) 106
DpnI GATC 1 cut(s) 348
DpnII GATC 1 cut(s) 346
Eam1104I CTCTTC 6 cut(s) 363, 636, 651, 668, 722, 731
EarI CTCTTC 6 cut(s) 363, 636, 651, 668, 722, 731
EciI GGCGGA 1 cut(s) 61
Ecl136II GAGCTC 1 cut(s) 309
Eco24I GRGCYC 2 cut(s) 207, 311
Eco31I GGTCTC 2 cut(s) 125, 248
Eco47I GGWCC 1 cut(s) 10
Eco53kI GAGCTC 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 309
EcoO109I RGGNCCY 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 156
EcoT38I GRGCYC 2 cut(s) 207, 311
EgeI GGCGCC 1 cut(s) 106
EheI GGCGCC 1 cut(s) 106
FaeI CATG 3 cut(s) 23, 316, 335
FaiI YATR 3 cut(s) 21, 314, 333
FaqI GGGAC 4 cut(s) 109, 281, 314, 587
FatI CATG 3 cut(s) 19, 312, 331
FauI CCCGC 1 cut(s) 374
Fnu4HI GCNGC 3 cut(s) 108, 120, 182
FokI GGATG 3 cut(s) 413, 596, 629
FriOI GRGCYC 2 cut(s) 207, 311
Fsp4HI GCNGC 3 cut(s) 108, 120, 182
GlaI GCGC 3 cut(s) 106, 154, 279
GluI GCNGC 3 cut(s) 108, 120, 182
GsuI CTGGAG 1 cut(s) 197
HaeII RGCGCY 1 cut(s) 108
HaeIII GGCC 3 cut(s) 161, 205, 489
HapII CCGG 2 cut(s) 275, 298
HgaI GACGC 2 cut(s) 111, 159
HhaI GCGC 3 cut(s) 107, 155, 280
Hin1I GRCGYC 2 cut(s) 105, 339
Hin1II CATG 3 cut(s) 23, 316, 335
Hin6I GCGC 3 cut(s) 105, 153, 278
HinP1I GCGC 3 cut(s) 105, 153, 278
HinfI GANTC 2 cut(s) 233, 385
HpaII CCGG 2 cut(s) 275, 298
HphI GGTGA 3 cut(s) 419, 463, 521
Hpy188I TCNGA 5 cut(s) 34, 149, 225, 232, 351
Hpy188III TCNNGA 1 cut(s) 332
Hpy99I CGWCG 3 cut(s) 153, 248, 251
HpyCH4III ACNGT 2 cut(s) 10, 329
HpyCH4IV ACGT 3 cut(s) 249, 303, 339
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 181, 202
HpySE526I ACGT 3 cut(s) 249, 303, 339
Hsp92I GRCGYC 2 cut(s) 105, 339
Hsp92II CATG 3 cut(s) 23, 316, 335
HspAI GCGC 3 cut(s) 105, 153, 278
KasI GGCGCC 1 cut(s) 104
Kzo9I GATC 1 cut(s) 346
LmnI GCTCC 5 cut(s) 172, 178, 189, 314, 636
LpnPI CCDG 8 cut(s) 124, 143, 148, 161, 170, 288, 311, 707
Lsp1109I GCAGC 1 cut(s) 193
LweI GCATC 2 cut(s) 510, 694
MaeII ACGT 3 cut(s) 249, 303, 339
MalI GATC 1 cut(s) 348
MboI GATC 1 cut(s) 346
MhlI GDGCHC 2 cut(s) 207, 311
MluCI AATT 4 cut(s) 391, 411, 492, 600
Mly113I GGCGCC 1 cut(s) 105
MlyI GAGTC 2 cut(s) 242, 394
MmeI TCCRAC 6 cut(s) 57, 75, 172, 210, 343, 666
MseI TTAA 1 cut(s) 603
MslI CAYNNNNRTG 1 cut(s) 330
MspI CCGG 2 cut(s) 275, 298
MspR9I CCNGG 2 cut(s) 158, 299
MvaI CCWGG 1 cut(s) 158
MvnI CGCG 3 cut(s) 122, 153, 359
MwoI GCNNNNNNNGC 3 cut(s) 116, 181, 202
NarI GGCGCC 1 cut(s) 105
NciI CCSGG 1 cut(s) 299
NdeII GATC 1 cut(s) 346
NlaIII CATG 3 cut(s) 23, 316, 335
NlaIV GGNNCC 3 cut(s) 106, 204, 205
PagI TCATGA 1 cut(s) 331
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PkrI GCNGC 3 cut(s) 109, 121, 183
Ple19I CGATCG 1 cut(s) 349
PleI GAGTC 2 cut(s) 241, 393
PluTI GGCGCC 1 cut(s) 108
PpsI GAGTC 2 cut(s) 241, 393
Psp124BI GAGCTC 1 cut(s) 311
Psp6I CCWGG 1 cut(s) 156
PspGI CCWGG 1 cut(s) 156
PspN4I GGNNCC 3 cut(s) 106, 204, 205
PspOMI GGGCCC 1 cut(s) 203
PspPI GGNCC 3 cut(s) 10, 203, 204
PvuI CGATCG 1 cut(s) 349
RseI CAYNNNNRTG 1 cut(s) 330
SacI GAGCTC 1 cut(s) 311
SaqAI TTAA 1 cut(s) 603
SatI GCNGC 3 cut(s) 108, 120, 182
Sau3AI GATC 1 cut(s) 346
Sau96I GGNCC 3 cut(s) 10, 203, 204
SchI GAGTC 2 cut(s) 242, 394
ScrFI CCNGG 2 cut(s) 158, 299
SduI GDGCHC 2 cut(s) 207, 311
SfaNI GCATC 2 cut(s) 510, 694
SfoI GGCGCC 1 cut(s) 106
SinI GGWCC 1 cut(s) 10
SmiMI CAYNNNNRTG 1 cut(s) 330
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
Sse9I AATT 4 cut(s) 391, 411, 492, 600
SsiI CCGC 8 cut(s) 72, 93, 108, 120, 258, 359, 381, 416
SspDI GGCGCC 1 cut(s) 104
SspI AATATT 1 cut(s) 472
SstI GAGCTC 1 cut(s) 311
StyD4I CCNGG 2 cut(s) 156, 297
TaaI ACNGT 2 cut(s) 10, 329
TaiI ACGT 3 cut(s) 252, 306, 342
TaqI TCGA 3 cut(s) 168, 293, 345
TasI AATT 4 cut(s) 391, 411, 492, 600
TauI GCSGC 2 cut(s) 110, 122
Tru1I TTAA 1 cut(s) 603
Tru9I TTAA 1 cut(s) 603
TseI GCWGC 1 cut(s) 181
TspDTI ATGAA 2 cut(s) 17, 600
TspGWI ACGGA 2 cut(s) 76, 498
VpaK11BI GGWCC 1 cut(s) 10
ZraI GACGTC 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.