MD03G1106700.v1.1

Nucleolar protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009144
Physical Location & Seq
Reverse (-)
9183850 .. 9184677
828 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1106700.v1.1.491

Sequence Viewer

Length: 828 bp
ATGAAAACGGTATCGGGGATCGTCCTCTCGTCGAAGCCAATCTCTCTCTCCAAAGCGACCTCCACTCTCTCTTCCTTCGTCTCCAGCGACACCGGCGCGTCGCAAGAGTTCAGCGTCTACCTCCGACGCACCTTGGCATCGTTCAAGGAGCTGAAGCAGTTGCACAAGGAGCTCAAATCGCCGAGATCGGAAAGGAAGCGGCCGCGGCGGCACAGGTCGGAGACCGAAAACGACGACGTAGAGATCGTCGGAGAGAATCCGGCGCAGAGTACCGGTGTGAGTGAGGTGAGTCATGGGCTTGAGTCCAAGCCGGAAACGCAGCGACAGGTAGTGGATGCCCAGGTGAAGAAGAAGGAGAGGAAGAAGGGTGAAGTTCGTAAATTTGGGGAAGATGAAGGTGGAATTTCAGAGATGGGAGGGGAGGGTGGTGAAAAGAAAGAGGCAGCTGATTCAGTAGAGGTACTTAGGACGAAGAAGAAGAAGAAGAAGAGGGACAATGTAGATAATTCTAATGAAGATGGGGATCGAATTGAGAAGGGAGATGGCGGGGTCAAAATTGCAGAGAGGGCGAATGGCGGTGTTGAAAAGAGAGAGCCGGCTGATTCAGTTGATACACAAAATAAGAAGAAGAAAAAGAGGGACAATGTTGGCAATTCCGGTGAAAACGGGGGTCAAATGAAGAAGGGAGATGGCGGGGCTAAAACAGGGAGCGAAGAGGTAAAGAAGGAAGAGAGTAAGAAGAGGAAGAGTGGGGTGTTAGAAGATGGAAGCTCTGGTGCTTTAGGAGGAGAGCAAGGGGACAAGAAAAAGAAGAGGAGGAAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

276

Amino Acids

29.97

Weight (kDa)

9.65

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 117
AccII CGCG 2 cut(s) 98, 205
AciI CCGC 7 cut(s) 199, 203, 205, 208, 546, 576, 693
AclWI GGATC 2 cut(s) 26, 531
AcoI YGGCCR 1 cut(s) 200
AcsI RAATTY 2 cut(s) 380, 402
AcuI CTGAAG 1 cut(s) 173
AfaI GTAC 2 cut(s) 271, 462
AgeI ACCGGT 1 cut(s) 272
AgsI TTSAA 2 cut(s) 145, 584
AjnI CCWGG 1 cut(s) 339
AluBI AGCT 4 cut(s) 151, 172, 446, 771
AluI AGCT 4 cut(s) 151, 172, 446, 771
Alw21I GWGCWC 1 cut(s) 174
Alw26I GTCTC 2 cut(s) 85, 215
AlwI GGATC 2 cut(s) 26, 531
AoxI GGCC 1 cut(s) 200
ApeKI GCWGC 2 cut(s) 319, 443
ApoI RAATTY 2 cut(s) 380, 402
AsiGI ACCGGT 1 cut(s) 272
AspLEI GCGC 2 cut(s) 98, 265
AsuHPI GGTGA 5 cut(s) 298, 355, 380, 440, 671
BanII GRGCYC 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 2 cut(s) 331, 455
BccI CCATC 5 cut(s) 406, 512, 536, 683, 758
BciT130I CCWGG 1 cut(s) 341
BcoDI GTCTC 2 cut(s) 85, 215
BglI GCCNNNNNGGC 1 cut(s) 208
BisI GCNGC 6 cut(s) 200, 203, 206, 209, 320, 444
BlsI GCNGC 6 cut(s) 201, 204, 207, 210, 321, 445
Bme1390I CCNGG 1 cut(s) 341
BmrFI CCNGG 1 cut(s) 341
BmsI GCATC 2 cut(s) 146, 325
BpmI CTGGAG 1 cut(s) 67
BpuEI CTTGAG 1 cut(s) 320
BsaBI GATNNNNATC 1 cut(s) 522
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 3 cut(s) 132, 203, 339
BsaWI WCCGGW 2 cut(s) 272, 656
BsaXI ACNNNNNCTCC 1 cut(s) 808
Bse118I RCCGGY 3 cut(s) 92, 272, 595
Bse8I GATNNNNATC 1 cut(s) 522
BseBI CCWGG 1 cut(s) 341
BseDI CCNNGG 3 cut(s) 132, 203, 339
BseGI GGATG 1 cut(s) 340
BseJI GATNNNNATC 1 cut(s) 522
BseRI GAGGAG 1 cut(s) 801
BseX3I CGGCCG 1 cut(s) 200
BseXI GCAGC 2 cut(s) 331, 455
Bsh1236I CGCG 2 cut(s) 98, 205
Bsh1285I CGRYCG 1 cut(s) 203
BshFI GGCC 1 cut(s) 202
BshTI ACCGGT 1 cut(s) 272
BsiEI CGRYCG 1 cut(s) 203
BsiHKAI GWGCWC 1 cut(s) 174
BsiSI CCGG 6 cut(s) 93, 260, 273, 311, 596, 657
BslFI GGGAC 3 cut(s) 506, 653, 812
BsmAI GTCTC 2 cut(s) 85, 215
BsmBI CGTCTC 1 cut(s) 85
BsmFI GGGAC 3 cut(s) 506, 653, 812
BsnI GGCC 1 cut(s) 202
Bso31I GGTCTC 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 4 cut(s) 18, 185, 243, 523
BspACI CCGC 7 cut(s) 199, 203, 205, 208, 546, 576, 693
BspANI GGCC 1 cut(s) 202
BspFNI CGCG 2 cut(s) 98, 205
BspPI GGATC 2 cut(s) 26, 531
BspTNI GGTCTC 1 cut(s) 215
BsrFI RCCGGY 3 cut(s) 92, 272, 595
BssAI RCCGGY 3 cut(s) 92, 272, 595
BssECI CCNNGG 3 cut(s) 132, 203, 339
BssMI GATC 4 cut(s) 18, 185, 243, 523
BssT1I CCWWGG 1 cut(s) 132
Bst2UI CCWGG 1 cut(s) 341
Bst4CI ACNGT 1 cut(s) 10
Bst6I CTCTTC 7 cut(s) 76, 482, 708, 723, 734, 740, 806
BstC8I GCNNGC 1 cut(s) 597
BstDEI CTNAG 1 cut(s) 464
BstDSI CCRYGG 1 cut(s) 203
BstF5I GGATG 1 cut(s) 340
BstFNI CGCG 2 cut(s) 98, 205
BstHHI GCGC 2 cut(s) 98, 265
BstKTI GATC 4 cut(s) 21, 188, 246, 526
BstMAI GTCTC 2 cut(s) 85, 215
BstMBI GATC 4 cut(s) 18, 185, 243, 523
BstMCI CGRYCG 1 cut(s) 203
BstMWI GCNNNNNNNGC 7 cut(s) 93, 169, 178, 205, 208, 316, 566
BstNI CCWGG 1 cut(s) 341
BstSCI CCNGG 1 cut(s) 339
BstUI CGCG 2 cut(s) 98, 205
BstV1I GCAGC 2 cut(s) 331, 455
BstZI CGGCCG 1 cut(s) 200
BsuRI GGCC 1 cut(s) 202
BtgI CCRYGG 1 cut(s) 203
BtsCI GGATG 1 cut(s) 340
Cac8I GCNNGC 1 cut(s) 597
CciNI GCGGCCGC 1 cut(s) 200
CfoI GCGC 2 cut(s) 98, 265
Cfr10I RCCGGY 3 cut(s) 92, 272, 595
Cfr42I CCGCGG 1 cut(s) 206
CseI GACGC 3 cut(s) 87, 103, 135
Csp6I GTAC 2 cut(s) 270, 461
CspAI ACCGGT 1 cut(s) 272
CviAII CATG 1 cut(s) 293
CviQI GTAC 2 cut(s) 270, 461
DdeI CTNAG 1 cut(s) 464
DpnI GATC 4 cut(s) 20, 187, 245, 525
DpnII GATC 4 cut(s) 18, 185, 243, 523
EaeI YGGCCR 1 cut(s) 200
EagI CGGCCG 1 cut(s) 200
Eam1104I CTCTTC 7 cut(s) 76, 482, 708, 723, 734, 740, 806
EarI CTCTTC 7 cut(s) 76, 482, 708, 723, 734, 740, 806
Ecl136II GAGCTC 1 cut(s) 172
EclXI CGGCCG 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 132
Eco24I GRGCYC 1 cut(s) 174
Eco31I GGTCTC 1 cut(s) 215
Eco52I CGGCCG 1 cut(s) 200
Eco53kI GAGCTC 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 172
EcoRII CCWGG 1 cut(s) 339
EcoT14I CCWWGG 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 132
Esp3I CGTCTC 1 cut(s) 85
FaeI CATG 1 cut(s) 296
FaiI YATR 1 cut(s) 294
FaqI GGGAC 3 cut(s) 506, 653, 812
FatI CATG 1 cut(s) 292
FauI CCCGC 2 cut(s) 539, 686
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 6 cut(s) 200, 203, 206, 209, 320, 444
FokI GGATG 1 cut(s) 347
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 6 cut(s) 200, 203, 206, 209, 320, 444
GlaI GCGC 2 cut(s) 97, 264
GluI GCNGC 6 cut(s) 200, 203, 206, 209, 320, 444
GsuI CTGGAG 1 cut(s) 67
HaeIII GGCC 1 cut(s) 202
HapII CCGG 6 cut(s) 93, 260, 273, 311, 596, 657
HgaI GACGC 3 cut(s) 87, 103, 135
HhaI GCGC 2 cut(s) 98, 265
Hin1II CATG 1 cut(s) 296
Hin6I GCGC 2 cut(s) 96, 263
HinP1I GCGC 2 cut(s) 96, 263
HinfI GANTC 5 cut(s) 256, 289, 302, 449, 602
HpaII CCGG 6 cut(s) 93, 260, 273, 311, 596, 657
HphI GGTGA 5 cut(s) 298, 355, 380, 440, 671
Hpy166II GTNNAC 1 cut(s) 118
Hpy188I TCNGA 5 cut(s) 125, 190, 220, 251, 409
Hpy8I GTNNAC 1 cut(s) 118
Hpy99I CGWCG 6 cut(s) 34, 103, 129, 236, 239, 251
HpyAV CCTTC 7 cut(s) 85, 346, 358, 389, 529, 676, 718
HpyCH4III ACNGT 1 cut(s) 10
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 2 cut(s) 163, 560
HpyF10VI GCNNNNNNNGC 7 cut(s) 93, 169, 178, 205, 208, 316, 566
HpyF3I CTNAG 1 cut(s) 464
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 1 cut(s) 296
HspAI GCGC 2 cut(s) 96, 263
KroI GCCGGC 1 cut(s) 595
KroNI GCCGGC 1 cut(s) 597
KspI CCGCGG 1 cut(s) 206
Kzo9I GATC 4 cut(s) 18, 185, 243, 523
LmnI GCTCC 3 cut(s) 148, 169, 708
Lsp1109I GCAGC 2 cut(s) 331, 455
LweI GCATC 2 cut(s) 146, 325
MaeII ACGT 1 cut(s) 237
MalI GATC 4 cut(s) 20, 187, 245, 525
MboI GATC 4 cut(s) 18, 185, 243, 523
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 6 cut(s) 380, 402, 505, 528, 555, 652
MlyI GAGTC 2 cut(s) 298, 311
MmeI TCCRAC 3 cut(s) 148, 198, 229
MroNI GCCGGC 1 cut(s) 595
MspA1I CMGCKG 2 cut(s) 205, 446
MspI CCGG 6 cut(s) 93, 260, 273, 311, 596, 657
MspR9I CCNGG 1 cut(s) 341
MvaI CCWGG 1 cut(s) 341
MvnI CGCG 2 cut(s) 98, 205
MwoI GCNNNNNNNGC 7 cut(s) 93, 169, 178, 205, 208, 316, 566
NaeI GCCGGC 1 cut(s) 597
NdeII GATC 4 cut(s) 18, 185, 243, 523
NgoMIV GCCGGC 1 cut(s) 595
NlaIII CATG 1 cut(s) 296
NmeAIII GCCGAG 1 cut(s) 207
NotI GCGGCCGC 1 cut(s) 200
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 243
PdiI GCCGGC 1 cut(s) 597
PfeI GAWTC 3 cut(s) 256, 449, 602
PinAI ACCGGT 1 cut(s) 272
PkrI GCNGC 6 cut(s) 201, 204, 207, 210, 321, 445
PleI GAGTC 2 cut(s) 297, 310
PpsI GAGTC 2 cut(s) 297, 310
Psp124BI GAGCTC 1 cut(s) 174
Psp6I CCWGG 1 cut(s) 339
PspGI CCWGG 1 cut(s) 339
PvuII CAGCTG 1 cut(s) 446
RsaI GTAC 2 cut(s) 271, 462
RsaNI GTAC 2 cut(s) 270, 461
SacI GAGCTC 1 cut(s) 174
SacII CCGCGG 1 cut(s) 206
SatI GCNGC 6 cut(s) 200, 203, 206, 209, 320, 444
Sau3AI GATC 4 cut(s) 18, 185, 243, 523
SchI GAGTC 2 cut(s) 298, 311
ScrFI CCNGG 1 cut(s) 341
SduI GDGCHC 1 cut(s) 174
SfaNI GCATC 2 cut(s) 146, 325
Sfr303I CCGCGG 1 cut(s) 206
SgrAI CRCCGGYG 1 cut(s) 92
SgrBI CCGCGG 1 cut(s) 206
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
Sse9I AATT 6 cut(s) 380, 402, 505, 528, 555, 652
SsiI CCGC 7 cut(s) 199, 203, 205, 208, 546, 576, 693
SstI GAGCTC 1 cut(s) 174
StyD4I CCNGG 1 cut(s) 339
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 1 cut(s) 10
TaiI ACGT 1 cut(s) 240
TaqI TCGA 2 cut(s) 32, 526
TaqII GACCGA 1 cut(s) 239
TasI AATT 6 cut(s) 380, 402, 505, 528, 555, 652
TauI GCSGC 4 cut(s) 202, 205, 208, 211
TfiI GAWTC 3 cut(s) 256, 449, 602
TseI GCWGC 2 cut(s) 319, 443
TspDTI ATGAA 4 cut(s) 17, 408, 528, 692
XapI RAATTY 2 cut(s) 380, 402
XmiI GTMKAC 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.