RchiOBHm_Chr5g0061881

Nucleolar protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0009144
Physical Location & Seq
Forward (+)
67493008 .. 67496402
3395 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33822

Sequence Viewer

Length: 777 bp
ATGAAGACAGTCCAAAGCCGGGTACTCTCCTCCGACCCAATCTCTCTCTCCGACGCCACCTCCTTCCTCTCCGACTTCAACAGGTCCGTCTGCGGCGGCGGGGGCGGCGCGTCGGTCGGCGCGTCCCACGAGACCTGCTGGTTCGTCCGACGCGCCTTGGCCTCATTCGAGGAGCTGGAGCAGCTCTATCGCAAGCTCAAGGATCCAAGGTCCGATCGAAAGGGAAGACACCCCGGGTCGGAGACTCGAAACGACGACGTAGAGACCACCGTTGGGCAGAGCTTCGTGGCGAGCCGGGACGTGAGTGCCAAGCCCAAAAGCCACCGTCAGCGGGAGAGTGATGACGTCAATCGGAAAGTTGAGGTTGAAGAACAGAGTAAGGGTGGGAATCGTATTTTGATGGAAGATGGAGGTGAGATTGCAGAGAGGGTAGGGGTAGAGATTGATGATAAGAAGAAGAAGAAAAAGAAGAGGGAGGAAGATGGTGAGAGTGAAATGGGAAACGGAGAGGTAAAGAGGGAGATTGGTTTTCGAGATTTCGAGCAAGATGAGTCACAGAATAAGAAGAAGAAGAAAAAGAAGAGGGACAATGTTGATGAAAAAACTCCACCCCAGAAGGAGGAAGATGGGGAAAGTGAAATGGGGAGTGAAGAGGTAAAGAGGCAAGAGAAGAAGAAGAAGAAGAAGAAGAAGAGGAAGAGTGAGGTGTTAGAGAATGGTGAGATTGATGCCTTGGAGGAACAACAGGAGAAGAGAAAGAAGAGGAGAAAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

258

Amino Acids

29.46

Weight (kDa)

9.14

Isoelectric Point (pI)

59.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 348
Acc36I ACCTGC 1 cut(s) 143
AccII CGCG 3 cut(s) 110, 122, 153
AciI CCGC 5 cut(s) 93, 96, 99, 105, 331
AclWI GGATC 2 cut(s) 197, 210
AcyI GRCGYC 2 cut(s) 54, 345
AfaI GTAC 1 cut(s) 24
AfiI CCNNNNNNNGG 5 cut(s) 19, 238, 273, 331, 619
AgsI TTSAA 2 cut(s) 79, 368
AjiI CACGTC 1 cut(s) 301
AluBI AGCT 4 cut(s) 175, 184, 196, 282
AluI AGCT 4 cut(s) 175, 184, 196, 282
Alw26I GTCTC 3 cut(s) 125, 236, 257
AlwI GGATC 2 cut(s) 197, 210
Ama87I CYCGRG 1 cut(s) 233
AoxI GGCC 1 cut(s) 159
ApeKI GCWGC 1 cut(s) 181
AspLEI GCGC 3 cut(s) 110, 122, 155
AspS9I GGNCC 2 cut(s) 84, 210
AsuC2I CCSGG 4 cut(s) 20, 234, 235, 296
AsuHPI GGTGA 3 cut(s) 425, 497, 731
AvaI CYCGRG 1 cut(s) 233
AvaII GGWCC 2 cut(s) 84, 210
BamHI GGATCC 1 cut(s) 202
BauI CACGAG 1 cut(s) 128
BbsI GAAGAC 2 cut(s) 11, 232
BbvI GCAGC 1 cut(s) 193
BccI CCATC 4 cut(s) 394, 401, 476, 620
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BcnI CCSGG 4 cut(s) 20, 234, 235, 296
BcoDI GTCTC 3 cut(s) 125, 236, 257
BfuAI ACCTGC 1 cut(s) 143
BisI GCNGC 4 cut(s) 94, 97, 106, 182
BlsI GCNGC 4 cut(s) 95, 98, 107, 183
Bme1390I CCNGG 4 cut(s) 20, 234, 235, 296
Bme18I GGWCC 2 cut(s) 84, 210
BmeT110I CYCGRG 1 cut(s) 233
BmgBI CACGTC 1 cut(s) 301
BmgT120I GGNCC 2 cut(s) 84, 210
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 4 cut(s) 20, 234, 235, 296
BmsI GCATC 1 cut(s) 718
BpiI GAAGAC 2 cut(s) 11, 232
BpmI CTGGAG 1 cut(s) 197
BpuEI CTTGAG 1 cut(s) 182
BpuMI CCSGG 4 cut(s) 20, 234, 235, 296
BsaHI GRCGYC 2 cut(s) 54, 345
BsaI GGTCTC 2 cut(s) 125, 257
BsaJI CCNNGG 5 cut(s) 156, 206, 232, 233, 732
BsaXI ACNNNNNCTCC 3 cut(s) 44, 74, 757
Bsc4I CCNNNNNNNGG 5 cut(s) 19, 238, 273, 331, 619
BseDI CCNNGG 5 cut(s) 156, 206, 232, 233, 732
BseLI CCNNNNNNNGG 5 cut(s) 19, 238, 273, 331, 619
BseRI GAGGAG 2 cut(s) 19, 185
BseXI GCAGC 1 cut(s) 193
Bsh1236I CGCG 3 cut(s) 110, 122, 153
Bsh1285I CGRYCG 2 cut(s) 117, 217
BshFI GGCC 1 cut(s) 161
BsiEI CGRYCG 2 cut(s) 117, 217
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 3 cut(s) 19, 234, 295
BslFI GGGAC 3 cut(s) 109, 311, 599
BslI CCNNNNNNNGG 5 cut(s) 19, 238, 273, 331, 619
BsmAI GTCTC 3 cut(s) 125, 236, 257
BsmFI GGGAC 3 cut(s) 109, 311, 599
BsnI GGCC 1 cut(s) 161
Bso31I GGTCTC 2 cut(s) 125, 257
BsoBI CYCGRG 1 cut(s) 233
Bsp143I GATC 2 cut(s) 202, 214
BspACI CCGC 5 cut(s) 93, 96, 99, 105, 331
BspANI GGCC 1 cut(s) 161
BspFNI CGCG 3 cut(s) 110, 122, 153
BspLI GGNNCC 1 cut(s) 204
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 2 cut(s) 197, 210
BspTNI GGTCTC 2 cut(s) 125, 257
BssECI CCNNGG 5 cut(s) 156, 206, 232, 233, 732
BssMI GATC 2 cut(s) 202, 214
BssNI GRCGYC 2 cut(s) 54, 345
BssSI CACGAG 1 cut(s) 128
BssT1I CCWWGG 3 cut(s) 156, 206, 732
Bst2BI CACGAG 1 cut(s) 128
Bst4CI ACNGT 3 cut(s) 10, 271, 326
Bst6I CTCTTC 7 cut(s) 464, 575, 645, 686, 692, 746, 755
BstACI GRCGYC 2 cut(s) 54, 345
BstC8I GCNNGC 2 cut(s) 194, 292
BstFNI CGCG 3 cut(s) 110, 122, 153
BstHHI GCGC 3 cut(s) 110, 122, 155
BstKTI GATC 2 cut(s) 205, 217
BstMAI GTCTC 3 cut(s) 125, 236, 257
BstMBI GATC 2 cut(s) 202, 214
BstMCI CGRYCG 2 cut(s) 117, 217
BstMWI GCNNNNNNNGC 3 cut(s) 102, 105, 181
BstSCI CCNGG 4 cut(s) 18, 232, 233, 294
BstUI CGCG 3 cut(s) 110, 122, 153
BstV1I GCAGC 1 cut(s) 193
BstV2I GAAGAC 2 cut(s) 11, 232
BstX2I RGATCY 1 cut(s) 202
BstYI RGATCY 1 cut(s) 202
BsuRI GGCC 1 cut(s) 161
BtrI CACGTC 1 cut(s) 301
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 194, 292
CfoI GCGC 3 cut(s) 110, 122, 155
Cfr13I GGNCC 2 cut(s) 84, 210
Cfr9I CCCGGG 1 cut(s) 233
CseI GACGC 4 cut(s) 62, 99, 111, 159
Csp6I GTAC 1 cut(s) 23
CviJI RGCY 9 cut(s) 18, 161, 175, 184, 196, 282, 294, 313, 321
CviKI_1 RGCY 9 cut(s) 18, 161, 175, 184, 196, 282, 294, 313, 321
CviQI GTAC 1 cut(s) 23
DpnI GATC 2 cut(s) 204, 216
DpnII GATC 2 cut(s) 202, 214
Eam1104I CTCTTC 7 cut(s) 464, 575, 645, 686, 692, 746, 755
EarI CTCTTC 7 cut(s) 464, 575, 645, 686, 692, 746, 755
Eco130I CCWWGG 3 cut(s) 156, 206, 732
Eco31I GGTCTC 2 cut(s) 125, 257
Eco47I GGWCC 2 cut(s) 84, 210
Eco88I CYCGRG 1 cut(s) 233
EcoT14I CCWWGG 3 cut(s) 156, 206, 732
ErhI CCWWGG 3 cut(s) 156, 206, 732
FaqI GGGAC 3 cut(s) 109, 311, 599
FauI CCCGC 2 cut(s) 92, 324
Fnu4HI GCNGC 4 cut(s) 94, 97, 106, 182
Fsp4HI GCNGC 4 cut(s) 94, 97, 106, 182
GlaI GCGC 3 cut(s) 109, 121, 154
GluI GCNGC 4 cut(s) 94, 97, 106, 182
GsuI CTGGAG 1 cut(s) 197
HaeIII GGCC 1 cut(s) 161
HapII CCGG 3 cut(s) 19, 234, 295
HgaI GACGC 4 cut(s) 62, 99, 111, 159
HhaI GCGC 3 cut(s) 110, 122, 155
Hin1I GRCGYC 2 cut(s) 54, 345
Hin6I GCGC 3 cut(s) 108, 120, 153
HinP1I GCGC 3 cut(s) 108, 120, 153
HinfI GANTC 3 cut(s) 244, 388, 551
HpaII CCGG 3 cut(s) 19, 234, 295
HphI GGTGA 3 cut(s) 425, 497, 731
Hpy188I TCNGA 7 cut(s) 34, 52, 73, 149, 214, 241, 354
Hpy188III TCNNGA 1 cut(s) 533
Hpy99I CGWCG 5 cut(s) 56, 115, 153, 257, 260
HpyAV CCTTC 2 cut(s) 73, 610
HpyCH4III ACNGT 3 cut(s) 10, 271, 326
HpyCH4IV ACGT 3 cut(s) 258, 300, 345
HpyCH4V TGCA 1 cut(s) 422
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 105, 181
HpySE526I ACGT 3 cut(s) 258, 300, 345
Hsp92I GRCGYC 2 cut(s) 54, 345
HspAI GCGC 3 cut(s) 108, 120, 153
Kzo9I GATC 2 cut(s) 202, 214
LmnI GCTCC 2 cut(s) 172, 178
LpnPI CCDG 9 cut(s) 32, 67, 124, 148, 161, 247, 308, 626, 731
Lsp1109I GCAGC 1 cut(s) 193
LweI GCATC 1 cut(s) 718
MaeII ACGT 3 cut(s) 258, 300, 345
MaeIII GTNAC 1 cut(s) 552
MalI GATC 2 cut(s) 204, 216
MboI GATC 2 cut(s) 202, 214
MflI RGATCY 1 cut(s) 202
MlyI GAGTC 2 cut(s) 238, 560
MmeI TCCRAC 5 cut(s) 57, 75, 96, 172, 219
MspA1I CMGCKG 1 cut(s) 331
MspI CCGG 3 cut(s) 19, 234, 295
MspR9I CCNGG 4 cut(s) 20, 234, 235, 296
MvnI CGCG 3 cut(s) 110, 122, 153
MwoI GCNNNNNNNGC 3 cut(s) 102, 105, 181
NciI CCSGG 4 cut(s) 20, 234, 235, 296
NdeII GATC 2 cut(s) 202, 214
NlaIV GGNNCC 1 cut(s) 204
NmuCI GTSAC 1 cut(s) 552
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PfeI GAWTC 1 cut(s) 388
PkrI GCNGC 4 cut(s) 95, 98, 107, 183
Ple19I CGATCG 1 cut(s) 217
PleI GAGTC 2 cut(s) 238, 559
PpsI GAGTC 2 cut(s) 238, 559
PspN4I GGNNCC 1 cut(s) 204
PspPI GGNCC 2 cut(s) 84, 210
PsuI RGATCY 1 cut(s) 202
PvuI CGATCG 1 cut(s) 217
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
SatI GCNGC 4 cut(s) 94, 97, 106, 182
Sau3AI GATC 2 cut(s) 202, 214
Sau96I GGNCC 2 cut(s) 84, 210
SchI GAGTC 2 cut(s) 238, 560
ScrFI CCNGG 4 cut(s) 20, 234, 235, 296
SfaNI GCATC 1 cut(s) 718
SinI GGWCC 2 cut(s) 84, 210
SmaI CCCGGG 1 cut(s) 235
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
SsiI CCGC 5 cut(s) 93, 96, 99, 105, 331
StyD4I CCNGG 4 cut(s) 18, 232, 233, 294
StyI CCWWGG 3 cut(s) 156, 206, 732
TaaI ACNGT 3 cut(s) 10, 271, 326
TaiI ACGT 3 cut(s) 261, 303, 348
TaqI TCGA 5 cut(s) 168, 217, 247, 532, 540
TaqII GACCGA 1 cut(s) 103
TauI GCSGC 3 cut(s) 96, 99, 108
TfiI GAWTC 1 cut(s) 388
TseFI GTSAC 1 cut(s) 552
TseI GCWGC 1 cut(s) 181
Tsp45I GTSAC 1 cut(s) 552
TspDTI ATGAA 2 cut(s) 17, 612
TspGWI ACGGA 2 cut(s) 76, 519
TspMI CCCGGG 1 cut(s) 233
VpaK11BI GGWCC 2 cut(s) 84, 210
XmaI CCCGGG 1 cut(s) 233
ZraI GACGTC 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.