Prupe.6G089500_v2.0.a1

Nucleolar protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0009144
Physical Location & Seq
Reverse (-)
6111586 .. 6113749
2164 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G089500.1

Sequence Viewer

Length: 945 bp
ATGACGGTCTCAGGGACCGTCCTCTCCAGAAAGCCAATCTCTCTCTCCAAAGCGACCTCGACTTTGTCCTCCTTCGTCTCCAGCGACACCGGCGCGTCGCAAGAGTTCAGCGTGTACCTCCAACGCGCCTTGGCATCGTTCAAGGAGCTGAAGCAGCTCCATAAGGAGCTCAAAGCGCCGAGGTCCCAACGCAGGCGATCGCGGCATAGGTCGGAGGCTGAAAATGACGACGCAGAGACCGTCGGAGAGAATCCGACTCGGAGTGTTGTGGTGAGTCAGGAAGTGAGTCATGGGCTCGAGTTAGAAACAAAGCCCAAGCCCAAAAGGCAGCGACAGGAGAGTAATAGCAATGGGAGGCTTGAGAATGAAGAACCCAATCGTCCGGTAGTGGATGCTGTTGTGAGGAGGAAGGAGGACGAGAAGAAGGAGAAGAAGGGTGAGTTTCTTAATTTCAGGGAACATAGAGGTGCAATTGCAGAGAGGGGAGGTGAGGGTGCTGAAAAGAGAGAGGCTTCTGATCCAGTAGAGGTGCATGACAAGAAGAAGAAAAAGAAGAGGGACAATGTTAGTAATTGTGATGGAAATGGGGATCATTTTGACAAGGGATATGGCGGGGGTAAAGTTGCAGAGGGGCCAGGGGAGGGCGTTGAAAGAAGAGAGGCAGCTGATTCAGCAGAGATGCATAATAAGAAAAAGAACAAAAAGAAGGGGGACACTGTTGGTAATTCCGTGGCAGTAGAGATGCATGACAAGAAGAAGAAGAAAAATAAGGAGGATAATGTTGGTAATTCGGATGAGAATGCAGTGCAAATTGAGAAGGGAGATGGTGAGGCTAAGATCAGAAACGAAGAGGTAAAGAAGGAAGAGGGAAAGAAGAGGAAGAGTGGGGTGTTAGATGCTATGGAGGAAAATCTGGATAAGAAAAAGAAGAGGAGGAAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

315

Amino Acids

35.14

Weight (kDa)

9.52

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 95, 126, 202
AciI CCGC 2 cut(s) 202, 612
AclWI GGATC 2 cut(s) 512, 597
AcuI CTGAAG 1 cut(s) 170
AfaI GTAC 1 cut(s) 116
AfiI CCNNNNNNNGG 2 cut(s) 192, 641
AgsI TTSAA 2 cut(s) 142, 650
AjnI CCWGG 1 cut(s) 634
AluBI AGCT 4 cut(s) 148, 157, 169, 665
AluI AGCT 4 cut(s) 148, 157, 169, 665
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 3 cut(s) 13, 82, 230
AlwI GGATC 2 cut(s) 512, 597
Ama87I CYCGRG 1 cut(s) 296
AoxI GGCC 1 cut(s) 632
ApeKI GCWGC 3 cut(s) 154, 328, 662
ArsI GACNNNNNNTTYG 2 cut(s) 46, 78
AsiSI GCGATCGC 1 cut(s) 200
AspLEI GCGC 3 cut(s) 95, 128, 178
AspS9I GGNCC 3 cut(s) 15, 183, 632
AsuHPI GGTGA 4 cut(s) 283, 449, 500, 839
AvaI CYCGRG 1 cut(s) 296
AvaII GGWCC 2 cut(s) 15, 183
BanII GRGCYC 2 cut(s) 171, 297
Bbv12I GWGCWC 1 cut(s) 171
BbvI GCAGC 3 cut(s) 166, 340, 674
BccI CCATC 2 cut(s) 572, 818
BciT130I CCWGG 1 cut(s) 636
BcoDI GTCTC 3 cut(s) 13, 82, 230
BfoI RGCGCY 1 cut(s) 179
BglI GCCNNNNNGGC 1 cut(s) 325
BisI GCNGC 4 cut(s) 155, 203, 329, 663
BlsI GCNGC 4 cut(s) 156, 204, 330, 664
Bme1390I CCNGG 1 cut(s) 636
Bme18I GGWCC 2 cut(s) 15, 183
BmeT110I CYCGRG 1 cut(s) 296
BmgT120I GGNCC 3 cut(s) 15, 183, 632
BmiI GGNNCC 3 cut(s) 16, 185, 633
BmrFI CCNGG 1 cut(s) 636
BmsI GCATC 5 cut(s) 143, 382, 669, 732, 886
BpmI CTGGAG 2 cut(s) 10, 64
BpuEI CTTGAG 1 cut(s) 380
BsaI GGTCTC 2 cut(s) 13, 230
BsaJI CCNNGG 4 cut(s) 129, 179, 635, 729
BsaWI WCCGGW 1 cut(s) 382
BsaXI ACNNNNNCTCC 1 cut(s) 925
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 641
Bse118I RCCGGY 1 cut(s) 89
Bse1I ACTGG 1 cut(s) 521
Bse3DI GCAATG 1 cut(s) 355
BseBI CCWGG 1 cut(s) 636
BseDI CCNNGG 4 cut(s) 129, 179, 635, 729
BseGI GGATG 2 cut(s) 397, 799
BseLI CCNNNNNNNGG 2 cut(s) 192, 641
BseMI GCAATG 1 cut(s) 355
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 1 cut(s) 521
BseRI GAGGAG 1 cut(s) 418
BseXI GCAGC 3 cut(s) 166, 340, 674
Bsh1236I CGCG 3 cut(s) 95, 126, 202
Bsh1285I CGRYCG 1 cut(s) 200
BshFI GGCC 1 cut(s) 634
BsiEI CGRYCG 1 cut(s) 200
BsiHKAI GWGCWC 1 cut(s) 171
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 2 cut(s) 90, 383
BslFI GGGAC 4 cut(s) 28, 169, 572, 725
BslI CCNNNNNNNGG 2 cut(s) 192, 641
BsmAI GTCTC 3 cut(s) 13, 82, 230
BsmBI CGTCTC 1 cut(s) 82
BsmFI GGGAC 4 cut(s) 28, 169, 572, 725
BsmI GAATGC 1 cut(s) 805
BsnI GGCC 1 cut(s) 634
Bso31I GGTCTC 2 cut(s) 13, 230
BsoBI CYCGRG 1 cut(s) 296
Bsp1286I GDGCHC 2 cut(s) 171, 297
Bsp143I GATC 4 cut(s) 197, 517, 589, 837
BspACI CCGC 2 cut(s) 202, 612
BspANI GGCC 1 cut(s) 634
BspCNI CTCAG 1 cut(s) 23
BspFNI CGCG 3 cut(s) 95, 126, 202
BspLI GGNNCC 3 cut(s) 16, 185, 633
BspPI GGATC 2 cut(s) 512, 597
BspTNI GGTCTC 2 cut(s) 13, 230
BsrDI GCAATG 1 cut(s) 355
BsrFI RCCGGY 1 cut(s) 89
BsrI ACTGG 1 cut(s) 521
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 4 cut(s) 129, 179, 635, 729
BssMI GATC 4 cut(s) 197, 517, 589, 837
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 1 cut(s) 636
Bst4CI ACNGT 4 cut(s) 7, 19, 241, 718
Bst6I CTCTTC 7 cut(s) 548, 649, 843, 858, 869, 875, 923
BstC8I GCNNGC 1 cut(s) 194
BstDEI CTNAG 2 cut(s) 10, 834
BstDSI CCRYGG 1 cut(s) 729
BstF5I GGATG 2 cut(s) 397, 799
BstFNI CGCG 3 cut(s) 95, 126, 202
BstH2I RGCGCY 1 cut(s) 179
BstHHI GCGC 3 cut(s) 95, 128, 178
BstKTI GATC 4 cut(s) 200, 520, 592, 840
BstMAI GTCTC 3 cut(s) 13, 82, 230
BstMBI GATC 4 cut(s) 197, 517, 589, 837
BstMCI CGRYCG 1 cut(s) 200
BstMWI GCNNNNNNNGC 6 cut(s) 90, 154, 175, 202, 325, 671
BstNI CCWGG 1 cut(s) 636
BstSCI CCNGG 1 cut(s) 634
BstUI CGCG 3 cut(s) 95, 126, 202
BstV1I GCAGC 3 cut(s) 166, 340, 674
BsuRI GGCC 1 cut(s) 634
BtgI CCRYGG 1 cut(s) 729
BtsCI GGATG 2 cut(s) 397, 799
BtsI GCAGTG 1 cut(s) 810
BtsIMutI CAGTG 2 cut(s) 714, 810
Cac8I GCNNGC 1 cut(s) 194
CfoI GCGC 3 cut(s) 95, 128, 178
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 3 cut(s) 15, 183, 632
CseI GACGC 2 cut(s) 84, 239
Csp6I GTAC 1 cut(s) 115
CviAII CATG 3 cut(s) 290, 533, 746
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 2 cut(s) 10, 834
DpnI GATC 4 cut(s) 199, 519, 591, 839
DpnII GATC 4 cut(s) 197, 517, 589, 837
Eam1104I CTCTTC 7 cut(s) 548, 649, 843, 858, 869, 875, 923
EarI CTCTTC 7 cut(s) 548, 649, 843, 858, 869, 875, 923
Ecl136II GAGCTC 1 cut(s) 169
Eco130I CCWWGG 1 cut(s) 129
Eco24I GRGCYC 2 cut(s) 171, 297
Eco31I GGTCTC 2 cut(s) 13, 230
Eco47I GGWCC 2 cut(s) 15, 183
Eco53kI GAGCTC 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 170
Eco88I CYCGRG 1 cut(s) 296
EcoICRI GAGCTC 1 cut(s) 169
EcoO109I RGGNCCY 1 cut(s) 183
EcoRII CCWGG 1 cut(s) 634
EcoT14I CCWWGG 1 cut(s) 129
EcoT22I ATGCAT 2 cut(s) 684, 747
EcoT38I GRGCYC 2 cut(s) 171, 297
ErhI CCWWGG 1 cut(s) 129
Esp3I CGTCTC 1 cut(s) 82
FaeI CATG 3 cut(s) 293, 536, 749
FaiI YATR 9 cut(s) 162, 207, 291, 462, 534, 609, 684, 747, 902
FaqI GGGAC 4 cut(s) 28, 169, 572, 725
FatI CATG 3 cut(s) 289, 532, 745
FauI CCCGC 1 cut(s) 605
Fnu4HI GCNGC 4 cut(s) 155, 203, 329, 663
FokI GGATG 2 cut(s) 404, 806
FriOI GRGCYC 2 cut(s) 171, 297
Fsp4HI GCNGC 4 cut(s) 155, 203, 329, 663
GlaI GCGC 3 cut(s) 94, 127, 177
GluI GCNGC 4 cut(s) 155, 203, 329, 663
GsuI CTGGAG 2 cut(s) 10, 64
HaeII RGCGCY 1 cut(s) 179
HaeIII GGCC 1 cut(s) 634
HapII CCGG 2 cut(s) 90, 383
HgaI GACGC 2 cut(s) 84, 239
HhaI GCGC 3 cut(s) 95, 128, 178
Hin1II CATG 3 cut(s) 293, 536, 749
Hin6I GCGC 3 cut(s) 93, 126, 176
HinP1I GCGC 3 cut(s) 93, 126, 176
HinfI GANTC 5 cut(s) 250, 256, 274, 286, 668
HpaII CCGG 2 cut(s) 90, 383
HphI GGTGA 4 cut(s) 283, 449, 500, 839
Hpy166II GTNNAC 1 cut(s) 115
Hpy188I TCNGA 7 cut(s) 214, 245, 255, 261, 517, 793, 842
Hpy188III TCNNGA 3 cut(s) 27, 278, 914
Hpy8I GTNNAC 1 cut(s) 115
Hpy99I CGWCG 3 cut(s) 100, 233, 245
HpyAV CCTTC 7 cut(s) 82, 403, 418, 427, 700, 811, 853
HpyCH4III ACNGT 4 cut(s) 7, 19, 241, 718
HpyCH4V TGCA 8 cut(s) 470, 476, 532, 626, 682, 745, 803, 808
HpyF10VI GCNNNNNNNGC 6 cut(s) 90, 154, 175, 202, 325, 671
HpyF3I CTNAG 2 cut(s) 10, 834
Hsp92II CATG 3 cut(s) 293, 536, 749
HspAI GCGC 3 cut(s) 93, 126, 176
Kzo9I GATC 4 cut(s) 197, 517, 589, 837
LmnI GCTCC 3 cut(s) 145, 162, 166
Lsp1109I GCAGC 3 cut(s) 166, 340, 674
LweI GCATC 5 cut(s) 143, 382, 669, 732, 886
MalI GATC 4 cut(s) 199, 519, 591, 839
MboI GATC 4 cut(s) 197, 517, 589, 837
MfeI CAATTG 1 cut(s) 471
MhlI GDGCHC 2 cut(s) 171, 297
MluCI AATT 6 cut(s) 448, 471, 571, 724, 787, 810
MlyI GAGTC 3 cut(s) 250, 283, 295
MmeI TCCRAC 4 cut(s) 145, 192, 223, 278
Mph1103I ATGCAT 2 cut(s) 684, 747
MseI TTAA 1 cut(s) 447
MslI CAYNNNNRTG 1 cut(s) 465
MspA1I CMGCKG 1 cut(s) 665
MspI CCGG 2 cut(s) 90, 383
MspR9I CCNGG 1 cut(s) 636
MunI CAATTG 1 cut(s) 471
Mva1269I GAATGC 1 cut(s) 805
MvaI CCWGG 1 cut(s) 636
MvnI CGCG 3 cut(s) 95, 126, 202
MwoI GCNNNNNNNGC 6 cut(s) 90, 154, 175, 202, 325, 671
NdeII GATC 4 cut(s) 197, 517, 589, 837
NlaIII CATG 3 cut(s) 293, 536, 749
NlaIV GGNNCC 3 cut(s) 16, 185, 633
NmeAIII GCCGAG 1 cut(s) 204
NsiI ATGCAT 2 cut(s) 684, 747
PaeR7I CTCGAG 1 cut(s) 296
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 237
PctI GAATGC 1 cut(s) 805
PfeI GAWTC 2 cut(s) 250, 668
PflFI GACNNNGTC 1 cut(s) 64
PkrI GCNGC 4 cut(s) 156, 204, 330, 664
Ple19I CGATCG 1 cut(s) 200
PleI GAGTC 3 cut(s) 250, 282, 294
PpsI GAGTC 3 cut(s) 250, 282, 294
PpuMI RGGWCCY 1 cut(s) 183
Psp124BI GAGCTC 1 cut(s) 171
Psp5II RGGWCCY 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 634
PspGI CCWGG 1 cut(s) 634
PspN4I GGNNCC 3 cut(s) 16, 185, 633
PspPI GGNCC 3 cut(s) 15, 183, 632
PspPPI RGGWCCY 1 cut(s) 183
PspXI VCTCGAGB 1 cut(s) 296
PsyI GACNNNGTC 1 cut(s) 64
PvuI CGATCG 1 cut(s) 200
PvuII CAGCTG 1 cut(s) 665
RgaI GCGATCGC 1 cut(s) 200
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
RseI CAYNNNNRTG 1 cut(s) 465
SacI GAGCTC 1 cut(s) 171
SaqAI TTAA 1 cut(s) 447
SatI GCNGC 4 cut(s) 155, 203, 329, 663
Sau3AI GATC 4 cut(s) 197, 517, 589, 837
Sau96I GGNCC 3 cut(s) 15, 183, 632
SchI GAGTC 3 cut(s) 250, 283, 295
ScrFI CCNGG 1 cut(s) 636
SduI GDGCHC 2 cut(s) 171, 297
SfaAI GCGATCGC 1 cut(s) 200
SfaNI GCATC 5 cut(s) 143, 382, 669, 732, 886
Sfr274I CTCGAG 1 cut(s) 296
SgfI GCGATCGC 1 cut(s) 200
SgrAI CRCCGGYG 1 cut(s) 89
SinI GGWCC 2 cut(s) 15, 183
SlaI CTCGAG 1 cut(s) 296
SmiMI CAYNNNNRTG 1 cut(s) 465
SmlI CTYRAG 2 cut(s) 296, 359
SmoI CTYRAG 2 cut(s) 296, 359
Sse9I AATT 6 cut(s) 448, 471, 571, 724, 787, 810
SsiI CCGC 2 cut(s) 202, 612
SstI GAGCTC 1 cut(s) 171
StyD4I CCNGG 1 cut(s) 634
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 4 cut(s) 7, 19, 241, 718
TaqI TCGA 2 cut(s) 59, 297
TasI AATT 6 cut(s) 448, 471, 571, 724, 787, 810
TauI GCSGC 1 cut(s) 205
TfiI GAWTC 2 cut(s) 250, 668
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TscAI CASTG 2 cut(s) 721, 810
TseI GCWGC 3 cut(s) 154, 328, 662
TspDTI ATGAA 1 cut(s) 381
TspGWI ACGGA 1 cut(s) 718
TspRI CASTG 2 cut(s) 721, 810
Tth111I GACNNNGTC 1 cut(s) 64
VpaK11BI GGWCC 2 cut(s) 15, 183
XhoI CTCGAG 1 cut(s) 296
Zsp2I ATGCAT 2 cut(s) 684, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.