FvH4_3g34750

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009518
Physical Location & Seq
Forward (+)
30072829 .. 30074853
2025 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g34750.t1

Sequence Viewer

Length: 759 bp
ATGAAAGGAATGGATCGCTTCCACTGTTCATCTTCTGCTTCCACGGCCATCATGGATCAAAGAGCCATAGTCCGCAGAGCCCACAGAGTCCCCATCAGCGACCATGTTCATGTTTATGATCGAAGAAGAAACCAATCCCATCTTCCTTTGATCCCTTGTTCATTTGATCAGTTACCAATTGATCCGAAGCCACTGTACGAAAAATGCAGAAGGAGTTTCTCTTCTGCGCAGTGTAGCGATAGAGATCAGCTACGTCGGAGAAGCTATGCTGAGTTCCATGAGCAAAGGAGGAAGAGTACTTCGTCCAGTTCTTCTCGGTATCGGACTAGTGACGATTCCACGGTGCCCTTCATCGACTGGTTATCGGAAACTGATGCAACGTTGGTTCCTTCTGATCACCATGTAGCTGCAGCAGCATCTAACCCTAGAATGCGCCTGATCATGAGATCGAATGATGAACATCGTCGTCCATCCCCAGCTCCATTAAGGTCTTCTTCCACTCGCTCTCGTAACCAGGTTGTGGTTCTGAGGGTGTCAGTGCATTGCAAAGGATGTGAAGAGAAACTGAGGAAGCATCTCTCTAAAATGGAAGGAGTTACATCGTTTAGTATAGATTTACCAACAAAGAAAGTCACGGTAATGGGAGACGTGACACCATTGGGTGTGCTCGCGAGTGTATCCAAGGTGAAGAAAGCCCAGCTTTGGTCTTCTCCAACATCTTCATCACCTTATTCACCATCTTCTCGATGGTCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

253

Amino Acids

28.52

Weight (kDa)

10.19

Isoelectric Point (pI)

73.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 176 - 222 2.5e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 228
AccB1I GGYRCC 1 cut(s) 343
AccB7I CCANNNNNTGG 1 cut(s) 520
AccII CGCG 1 cut(s) 671
AciI CCGC 1 cut(s) 73
AclI AACGTT 1 cut(s) 380
AclWI GGATC 4 cut(s) 21, 63, 145, 176
AcoI YGGCCR 1 cut(s) 45
AfaI GTAC 2 cut(s) 197, 298
AfiI CCNNNNNNNGG 2 cut(s) 520, 702
AhlI ACTAGT 1 cut(s) 326
AjiI CACGTC 1 cut(s) 649
AjnI CCWGG 1 cut(s) 513
AjuI GAANNNNNNNTTGG 2 cut(s) 126, 158
AluBI AGCT 5 cut(s) 250, 264, 407, 479, 700
AluI AGCT 5 cut(s) 250, 264, 407, 479, 700
Alw21I GWGCWC 1 cut(s) 669
Alw26I GTCTC 1 cut(s) 639
AlwI GGATC 4 cut(s) 21, 63, 145, 176
AoxI GGCC 1 cut(s) 45
ApeKI GCWGC 3 cut(s) 407, 410, 413
AspLEI GCGC 2 cut(s) 229, 435
AsuHPI GGTGA 4 cut(s) 389, 697, 717, 726
BaeGI GKGCMC 1 cut(s) 348
BanI GGYRCC 1 cut(s) 343
BanII GRGCYC 1 cut(s) 82
BarI GAAGNNNNNNTAC 2 cut(s) 179, 211
BbsI GAAGAC 2 cut(s) 483, 699
Bbv12I GWGCWC 1 cut(s) 669
BbvI GCAGC 3 cut(s) 394, 422, 425
BccI CCATC 6 cut(s) 56, 101, 147, 478, 741, 745
BceAI ACGGC 1 cut(s) 60
BciT130I CCWGG 1 cut(s) 515
BciVI GTATCC 1 cut(s) 688
BclI TGATCA 3 cut(s) 166, 394, 438
BcoDI GTCTC 1 cut(s) 639
BcuI ACTAGT 1 cut(s) 326
BfaI CTAG 2 cut(s) 327, 426
BfmI CTRYAG 1 cut(s) 408
BfuI GTATCC 1 cut(s) 688
BisI GCNGC 3 cut(s) 408, 411, 414
BlsI GCNGC 3 cut(s) 409, 412, 415
BmcAI AGTACT 1 cut(s) 298
Bme1390I CCNGG 1 cut(s) 515
BmgBI CACGTC 1 cut(s) 649
BmiI GGNNCC 2 cut(s) 345, 387
BmrFI CCNGG 1 cut(s) 515
BmsI GCATC 3 cut(s) 364, 425, 583
BpiI GAAGAC 2 cut(s) 483, 699
BsaBI GATNNNNATC 2 cut(s) 243, 459
BsaJI CCNNGG 3 cut(s) 42, 339, 681
BsaXI ACNNNNNCTCC 2 cut(s) 280, 310
Bsc4I CCNNNNNNNGG 2 cut(s) 520, 702
Bse1I ACTGG 2 cut(s) 306, 362
Bse3DI GCAATG 1 cut(s) 541
Bse8I GATNNNNATC 2 cut(s) 243, 459
BseBI CCWGG 1 cut(s) 515
BseDI CCNNGG 3 cut(s) 42, 339, 681
BseGI GGATG 2 cut(s) 470, 557
BseJI GATNNNNATC 2 cut(s) 243, 459
BseLI CCNNNNNNNGG 2 cut(s) 520, 702
BseMI GCAATG 1 cut(s) 541
BseMII CTCAG 3 cut(s) 261, 518, 557
BseNI ACTGG 2 cut(s) 306, 362
BseSI GKGCMC 1 cut(s) 348
BseXI GCAGC 3 cut(s) 394, 422, 425
BseYI CCCAGC 2 cut(s) 475, 696
Bsh1236I CGCG 1 cut(s) 671
BshFI GGCC 1 cut(s) 47
BshNI GGYRCC 1 cut(s) 343
BsiHKAI GWGCWC 1 cut(s) 669
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 2 cut(s) 520, 702
BsmAI GTCTC 1 cut(s) 639
BsmBI CGTCTC 1 cut(s) 639
BsmFI GGGAC 1 cut(s) 74
BsmI GAATGC 1 cut(s) 435
BsnI GGCC 1 cut(s) 47
Bsp1286I GDGCHC 3 cut(s) 82, 348, 669
Bsp68I TCGCGA 1 cut(s) 671
BspACI CCGC 1 cut(s) 73
BspANI GGCC 1 cut(s) 47
BspCNI CTCAG 3 cut(s) 262, 519, 558
BspFNI CGCG 1 cut(s) 671
BspHI TCATGA 1 cut(s) 441
BspLI GGNNCC 2 cut(s) 345, 387
BspMAI CTGCAG 1 cut(s) 412
BspPI GGATC 4 cut(s) 21, 63, 145, 176
BspT107I GGYRCC 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 541
BsrI ACTGG 2 cut(s) 306, 362
BssECI CCNNGG 3 cut(s) 42, 339, 681
BssT1I CCWWGG 1 cut(s) 681
Bst2UI CCWGG 1 cut(s) 515
Bst4CI ACNGT 4 cut(s) 26, 195, 343, 637
Bst6I CTCTTC 3 cut(s) 226, 287, 552
BstC8I GCNNGC 1 cut(s) 669
BstDEI CTNAG 3 cut(s) 270, 527, 566
BstDSI CCRYGG 2 cut(s) 42, 339
BstF5I GGATG 2 cut(s) 470, 557
BstFNI CGCG 1 cut(s) 671
BstHHI GCGC 2 cut(s) 229, 435
BstMAI GTCTC 1 cut(s) 639
BstMWI GCNNNNNNNGC 2 cut(s) 44, 413
BstNI CCWGG 1 cut(s) 515
BstSCI CCNGG 1 cut(s) 513
BstSFI CTRYAG 1 cut(s) 408
BstSLI GKGCMC 1 cut(s) 348
BstUI CGCG 1 cut(s) 671
BstV1I GCAGC 3 cut(s) 394, 422, 425
BstV2I GAAGAC 2 cut(s) 483, 699
BsuI GTATCC 1 cut(s) 688
BsuRI GGCC 1 cut(s) 47
BtgI CCRYGG 2 cut(s) 42, 339
BtrI CACGTC 1 cut(s) 649
BtsCI GGATG 2 cut(s) 470, 557
BtsI GCAGTG 1 cut(s) 236
BtsIMutI CAGTG 4 cut(s) 22, 191, 236, 543
BtuMI TCGCGA 1 cut(s) 671
Cac8I GCNNGC 1 cut(s) 669
CciI TCATGA 1 cut(s) 441
CfoI GCGC 2 cut(s) 229, 435
CsiI ACCWGGT 1 cut(s) 513
Csp6I GTAC 2 cut(s) 196, 297
CviAII CATG 7 cut(s) 52, 104, 110, 278, 401, 442, 756
CviQI GTAC 2 cut(s) 196, 297
DdeI CTNAG 3 cut(s) 270, 527, 566
EaeI YGGCCR 1 cut(s) 45
Eam1104I CTCTTC 3 cut(s) 226, 287, 552
EarI CTCTTC 3 cut(s) 226, 287, 552
Eco130I CCWWGG 1 cut(s) 681
Eco24I GRGCYC 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 513
EcoT14I CCWWGG 1 cut(s) 681
EcoT38I GRGCYC 1 cut(s) 82
ErhI CCWWGG 1 cut(s) 681
Esp3I CGTCTC 1 cut(s) 639
FaeI CATG 7 cut(s) 55, 107, 113, 281, 404, 445, 759
FalI AAGNNNNNCTT 4 cut(s) 478, 510, 684, 716
FaqI GGGAC 1 cut(s) 74
FatI CATG 7 cut(s) 51, 103, 109, 277, 400, 441, 755
FbaI TGATCA 3 cut(s) 166, 394, 438
Fnu4HI GCNGC 3 cut(s) 408, 411, 414
FokI GGATG 2 cut(s) 457, 564
FriOI GRGCYC 1 cut(s) 82
Fsp4HI GCNGC 3 cut(s) 408, 411, 414
FspBI CTAG 2 cut(s) 327, 426
FspI TGCGCA 1 cut(s) 228
GlaI GCGC 2 cut(s) 228, 434
GluI GCNGC 3 cut(s) 408, 411, 414
GsaI CCCAGC 2 cut(s) 479, 700
HaeIII GGCC 1 cut(s) 47
HhaI GCGC 2 cut(s) 229, 435
Hin1II CATG 7 cut(s) 55, 107, 113, 281, 404, 445, 759
Hin6I GCGC 2 cut(s) 227, 433
HinP1I GCGC 2 cut(s) 227, 433
HinfI GANTC 2 cut(s) 87, 335
HphI GGTGA 4 cut(s) 389, 697, 717, 726
Hpy188I TCNGA 6 cut(s) 186, 258, 324, 367, 394, 528
Hpy188III TCNNGA 3 cut(s) 442, 670, 744
Hpy99I CGWCG 2 cut(s) 258, 468
HpyAV CCTTC 4 cut(s) 204, 358, 399, 584
HpyCH4III ACNGT 4 cut(s) 26, 195, 343, 637
HpyCH4IV ACGT 3 cut(s) 253, 380, 648
HpyCH4V TGCA 6 cut(s) 207, 377, 410, 541, 546, 755
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 413
HpyF3I CTNAG 3 cut(s) 270, 527, 566
HpySE526I ACGT 3 cut(s) 253, 380, 648
Hsp92II CATG 7 cut(s) 55, 107, 113, 281, 404, 445, 759
HspAI GCGC 2 cut(s) 227, 433
Ksp22I TGATCA 3 cut(s) 166, 394, 438
LmnI GCTCC 1 cut(s) 484
LpnPI CCDG 7 cut(s) 319, 343, 449, 489, 500, 527, 710
Lsp1109I GCAGC 3 cut(s) 394, 422, 425
LweI GCATC 3 cut(s) 364, 425, 583
MabI ACCWGGT 1 cut(s) 513
MaeI CTAG 2 cut(s) 327, 426
MaeII ACGT 3 cut(s) 253, 380, 648
MaeIII GTNAC 6 cut(s) 171, 329, 509, 595, 631, 649
MfeI CAATTG 1 cut(s) 177
MhlI GDGCHC 3 cut(s) 82, 348, 669
MluCI AATT 1 cut(s) 177
MlyI GAGTC 1 cut(s) 96
MmeI TCCRAC 2 cut(s) 236, 737
MnlI CCTC 3 cut(s) 282, 522, 561
MseI TTAA 1 cut(s) 485
MslI CAYNNNNRTG 3 cut(s) 108, 114, 638
MspR9I CCNGG 1 cut(s) 515
MunI CAATTG 1 cut(s) 177
Mva1269I GAATGC 1 cut(s) 435
MvaI CCWGG 1 cut(s) 515
MvnI CGCG 1 cut(s) 671
MwoI GCNNNNNNNGC 2 cut(s) 44, 413
NlaIII CATG 7 cut(s) 55, 107, 113, 281, 404, 445, 759
NlaIV GGNNCC 2 cut(s) 345, 387
NmuCI GTSAC 3 cut(s) 329, 631, 649
NruI TCGCGA 1 cut(s) 671
NsbI TGCGCA 1 cut(s) 228
PagI TCATGA 1 cut(s) 441
PctI GAATGC 1 cut(s) 435
PfeI GAWTC 1 cut(s) 335
PflMI CCANNNNNTGG 1 cut(s) 520
PkrI GCNGC 3 cut(s) 409, 412, 415
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
Psp1406I AACGTT 1 cut(s) 380
Psp6I CCWGG 1 cut(s) 513
PspFI CCCAGC 2 cut(s) 475, 696
PspGI CCWGG 1 cut(s) 513
PspN4I GGNNCC 2 cut(s) 345, 387
PstI CTGCAG 1 cut(s) 412
RruI TCGCGA 1 cut(s) 671
RsaI GTAC 2 cut(s) 197, 298
RsaNI GTAC 2 cut(s) 196, 297
RseI CAYNNNNRTG 3 cut(s) 108, 114, 638
SaqAI TTAA 1 cut(s) 485
SatI GCNGC 3 cut(s) 408, 411, 414
ScaI AGTACT 1 cut(s) 298
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 1 cut(s) 515
SduI GDGCHC 3 cut(s) 82, 348, 669
SexAI ACCWGGT 1 cut(s) 513
SfaNI GCATC 3 cut(s) 364, 425, 583
SfcI CTRYAG 1 cut(s) 408
SmiMI CAYNNNNRTG 3 cut(s) 108, 114, 638
SpeI ACTAGT 1 cut(s) 326
Sse9I AATT 1 cut(s) 177
SsiI CCGC 1 cut(s) 73
SspMI CTAG 2 cut(s) 327, 426
StyD4I CCNGG 1 cut(s) 513
StyI CCWWGG 1 cut(s) 681
TaaI ACNGT 4 cut(s) 26, 195, 343, 637
TaiI ACGT 3 cut(s) 256, 383, 651
TaqI TCGA 4 cut(s) 121, 354, 449, 745
TasI AATT 1 cut(s) 177
TatI WGTACW 1 cut(s) 296
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 1 cut(s) 485
Tru9I TTAA 1 cut(s) 485
TscAI CASTG 4 cut(s) 29, 198, 236, 543
TseFI GTSAC 3 cut(s) 329, 631, 649
TseI GCWGC 3 cut(s) 407, 410, 413
Tsp45I GTSAC 3 cut(s) 329, 631, 649
TspDTI ATGAA 7 cut(s) 17, 18, 98, 150, 340, 471, 711
TspRI CASTG 4 cut(s) 29, 198, 236, 543
Van91I CCANNNNNTGG 1 cut(s) 520
XcmI CCANNNNNNNNNTGG 2 cut(s) 49, 744
XspI CTAG 2 cut(s) 327, 426
ZrmI AGTACT 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.