RLG00000035560

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0009518
Physical Location & Seq
Forward (+)
68580990 .. 68584641
3652 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035560

Sequence Viewer

Length: 1389 bp
ATGAAGGGAACGGATCGTTTCTTCTGTGCATCTTCTGCTTCCACAGCCATCATGGATCAAAGAGCCATAGTCCGCCGAGCCCACAGAGTCCCCATTAGCGACCATGTTTATGTTCATGATCGAAGAAAAAACCAACCCCATCTTCCTATGATCCCTTGTTCATTTGATCAGTTACCAATTGATCCGAAACCACTGTACGAAAGATGCAGAAGGAGTTTTTCTTCTGCGCAGAGTAGCGATAGAGATCAGCTACGTCGGAAAAGCTCTGCTGACATCCATGAGCAAAGAAGAAAAAGTACTTCCTCCACTTCTCGGTATCGGATTAGTGACGATTCCACAGTACCCTTCATCGACTGGTTATCGAAAACTGACGCAACGTTGGTTCCTTCTGATCATCATAAAGAAGCAGCTTCTAACCCTAGACTGCGCCTGATCATGAGATCGAATGATGAGTATCGTCGTCCATCCCCAGCTCCATTAAGGTCGTCTTCCACTCGCTCTAGTAACCAGGTTGTGGTTCTGAGGGTGTCAGTGCATTGCAAGGGATGTGAAGAAAAACTGAGAAAGCATCTCTCTAAAATGGAAGGAGTTGCATCATTTAGTATAGATTTACCAACAAAGAAAGTCACGGTCATCGGAGACGTGACACCATTTGGTGTGCTCGCGAGTGTATCCAAGTACATACAAATATCCTTGTTCTTATTTCTCGGCTCTTTTGGACCATCTCTTTTGAAGCTTTTCTACGAATATGTTGCCTCTATAACATTCATGTTACCAAAACGTTCAATCGAAAGCAGTTTTCTTTCATCACTGGCCAAAAAGAACCCCATGTATGCCATCATCAAAATCACATCATCCATCAAGTTGTTCGACTTGACTTGCCCACTGGAACTTGCTATACCTCATAGTTCCTCACCCAACATGGAACTTGCTATACCTCATAGTTCCTCACCCAACATGGAACTTGCTGTACATAAGACTTGTCTACTGAAGAATATCTTTGTTAAGAATCTCACCTTCATCTTTTTGAGTAAGAGTATAAGCTCTGAGGCGGCGGGCATATACAACTGGTCATCTTGGACCACTGAGGGTTTGAGGCAGTGTGGTAGCGCACCGCCTTCACCTCCGAATGACGATGACGTCGACGGCGGTGATACATCGGTGAAGGTGCAGATCCACGATGACCTCAAACACCGCTTCGTCGTTTCGTATCAAAACGAAGTAGAATCGCGCTGCGACGACGGCCAGGAGTTTATGGTCGATAAGCTTCGCAGGCTCCAAGTCGAGGAGCTCCAGCGCGAGGTGAAGAGGATGGAGGAGGAGAACGAAGATCGCAGCTTGAAGGCGGCGAAGGCGGAGCAGCCGGCTCATGCTCGTCTCTTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

463

Amino Acids

52.3

Weight (kDa)

9.2

Isoelectric Point (pI)

57.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 174 - 217 2.1e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1139
AatII GACGTC 1 cut(s) 1143
Acc16I TGCGCA 1 cut(s) 228
AccB7I CCANNNNNTGG 1 cut(s) 514
AccI GTMKAC 2 cut(s) 985, 1143
AccII CGCG 3 cut(s) 665, 1231, 1299
AciI CCGC 8 cut(s) 73, 1052, 1055, 1115, 1149, 1195, 1346, 1355
AclI AACGTT 2 cut(s) 377, 781
AclWI GGATC 5 cut(s) 21, 63, 145, 176, 1168
AcoI YGGCCR 2 cut(s) 813, 1243
AcuI CTGAAG 1 cut(s) 1010
AcyI GRCGYC 1 cut(s) 1140
AfaI GTAC 5 cut(s) 197, 298, 342, 680, 972
AfiI CCNNNNNNNGG 4 cut(s) 312, 514, 1285, 1300
AgsI TTSAA 3 cut(s) 733, 786, 1342
AjiI CACGTC 1 cut(s) 643
AjnI CCWGG 2 cut(s) 507, 1245
AjuI GAANNNNNNNTTGG 2 cut(s) 126, 158
AluBI AGCT 9 cut(s) 250, 264, 410, 473, 736, 1044, 1267, 1291, 1338
AluI AGCT 9 cut(s) 250, 264, 410, 473, 736, 1044, 1267, 1291, 1338
Alw21I GWGCWC 2 cut(s) 663, 1293
Alw26I GTCTC 2 cut(s) 633, 1382
AlwI GGATC 5 cut(s) 21, 63, 145, 176, 1168
AoxI GGCC 2 cut(s) 813, 1243
ApeKI GCWGC 4 cut(s) 407, 1233, 1335, 1360
Asp700I GAANNNNTTC 1 cut(s) 737
AspLEI GCGC 5 cut(s) 229, 429, 1112, 1233, 1299
AspS9I GGNCC 2 cut(s) 719, 1080
AsuHPI GGTGA 7 cut(s) 906, 942, 1006, 1113, 1163, 1174, 1315
AvaII GGWCC 2 cut(s) 719, 1080
BaeI ACNNNNGTAYC 2 cut(s) 1146, 1179
BalI TGGCCA 1 cut(s) 815
BanII GRGCYC 2 cut(s) 82, 1293
BarI GAAGNNNNNNTAC 4 cut(s) 280, 312, 725, 757
BbsI GAAGAC 1 cut(s) 480
Bbv12I GWGCWC 2 cut(s) 663, 1293
BbvI GCAGC 4 cut(s) 419, 1220, 1347, 1372
BccI CCATC 7 cut(s) 56, 147, 472, 730, 845, 866, 1306
BceAI ACGGC 2 cut(s) 1162, 1258
BcgI CGANNNNNNTGC 2 cut(s) 734, 768
BciT130I CCWGG 2 cut(s) 509, 1247
BciVI GTATCC 1 cut(s) 682
BclI TGATCA 3 cut(s) 166, 391, 432
BcoDI GTCTC 2 cut(s) 633, 1382
BfaI CTAG 2 cut(s) 420, 501
BfuI GTATCC 1 cut(s) 682
BisI GCNGC 6 cut(s) 408, 1053, 1234, 1336, 1347, 1361
BlsI GCNGC 6 cut(s) 409, 1054, 1235, 1337, 1348, 1362
BmcAI AGTACT 1 cut(s) 298
Bme1390I CCNGG 2 cut(s) 509, 1247
Bme18I GGWCC 2 cut(s) 719, 1080
BmgBI CACGTC 1 cut(s) 643
BmgT120I GGNCC 2 cut(s) 719, 1080
BmiI GGNNCC 2 cut(s) 384, 1277
BmrFI CCNGG 2 cut(s) 509, 1247
BmsI GCATC 4 cut(s) 38, 194, 577, 602
BpiI GAAGAC 1 cut(s) 480
BpmI CTGGAG 1 cut(s) 1277
BsaBI GATNNNNATC 2 cut(s) 243, 453
BsaHI GRCGYC 1 cut(s) 1140
Bsc4I CCNNNNNNNGG 4 cut(s) 312, 514, 1285, 1300
Bse118I RCCGGY 1 cut(s) 1363
Bse1I ACTGG 4 cut(s) 359, 816, 891, 1073
Bse3DI GCAATG 1 cut(s) 535
Bse8I GATNNNNATC 2 cut(s) 243, 453
BseBI CCWGG 2 cut(s) 509, 1247
BseGI GGATG 5 cut(s) 273, 464, 551, 854, 1317
BseJI GATNNNNATC 2 cut(s) 243, 453
BseLI CCNNNNNNNGG 4 cut(s) 312, 514, 1285, 1300
BseMI GCAATG 1 cut(s) 535
BseMII CTCAG 4 cut(s) 512, 551, 1038, 1077
BseNI ACTGG 4 cut(s) 359, 816, 891, 1073
BseRI GAGGAG 3 cut(s) 1301, 1331, 1334
BseXI GCAGC 4 cut(s) 419, 1220, 1347, 1372
BseYI CCCAGC 1 cut(s) 469
BsgI GTGCAG 1 cut(s) 1190
Bsh1236I CGCG 3 cut(s) 665, 1231, 1299
BshFI GGCC 2 cut(s) 815, 1245
BsiHKAI GWGCWC 2 cut(s) 663, 1293
BsiSI CCGG 1 cut(s) 1364
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 4 cut(s) 312, 514, 1285, 1300
BsmAI GTCTC 2 cut(s) 633, 1382
BsmBI CGTCTC 2 cut(s) 633, 1382
BsmFI GGGAC 1 cut(s) 74
BsnI GGCC 2 cut(s) 815, 1245
Bsp1286I GDGCHC 3 cut(s) 82, 663, 1293
Bsp1407I TGTACA 1 cut(s) 970
Bsp68I TCGCGA 1 cut(s) 665
BspACI CCGC 8 cut(s) 73, 1052, 1055, 1115, 1149, 1195, 1346, 1355
BspANI GGCC 2 cut(s) 815, 1245
BspCNI CTCAG 4 cut(s) 513, 552, 1039, 1078
BspFNI CGCG 3 cut(s) 665, 1231, 1299
BspHI TCATGA 2 cut(s) 115, 435
BspLI GGNNCC 2 cut(s) 384, 1277
BspPI GGATC 5 cut(s) 21, 63, 145, 176, 1168
BsrDI GCAATG 1 cut(s) 535
BsrFI RCCGGY 1 cut(s) 1363
BsrGI TGTACA 1 cut(s) 970
BsrI ACTGG 4 cut(s) 359, 816, 891, 1073
BssAI RCCGGY 1 cut(s) 1363
BssNI GRCGYC 1 cut(s) 1140
Bst2UI CCWGG 2 cut(s) 509, 1247
Bst4CI ACNGT 3 cut(s) 195, 340, 631
Bst6I CTCTTC 1 cut(s) 1301
BstACI GRCGYC 1 cut(s) 1140
BstAPI GCANNNNNTGC 1 cut(s) 35
BstAUI TGTACA 1 cut(s) 970
BstC8I GCNNGC 4 cut(s) 663, 1057, 1274, 1365
BstDEI CTNAG 4 cut(s) 521, 560, 1047, 1086
BstF5I GGATG 5 cut(s) 273, 464, 551, 854, 1317
BstFNI CGCG 3 cut(s) 665, 1231, 1299
BstHHI GCGC 5 cut(s) 229, 429, 1112, 1233, 1299
BstMAI GTCTC 2 cut(s) 633, 1382
BstMWI GCNNNNNNNGC 5 cut(s) 35, 44, 1242, 1273, 1352
BstNI CCWGG 2 cut(s) 509, 1247
BstSCI CCNGG 2 cut(s) 507, 1245
BstUI CGCG 3 cut(s) 665, 1231, 1299
BstV1I GCAGC 4 cut(s) 419, 1220, 1347, 1372
BstV2I GAAGAC 1 cut(s) 480
BstX2I RGATCY 1 cut(s) 1173
BstYI RGATCY 1 cut(s) 1173
BsuI GTATCC 1 cut(s) 682
BsuRI GGCC 2 cut(s) 815, 1245
BtrI CACGTC 1 cut(s) 643
BtsCI GGATG 5 cut(s) 273, 464, 551, 854, 1317
BtsI GCAGTG 1 cut(s) 1106
BtsIMutI CAGTG 6 cut(s) 191, 537, 809, 884, 1083, 1106
BtuMI TCGCGA 1 cut(s) 665
Cac8I GCNNGC 4 cut(s) 663, 1057, 1274, 1365
CciI TCATGA 2 cut(s) 115, 435
CfoI GCGC 5 cut(s) 229, 429, 1112, 1233, 1299
Cfr10I RCCGGY 1 cut(s) 1363
Cfr13I GGNCC 2 cut(s) 719, 1080
CseI GACGC 1 cut(s) 380
CsiI ACCWGGT 1 cut(s) 507
Csp6I GTAC 5 cut(s) 196, 297, 341, 679, 971
CviQI GTAC 5 cut(s) 196, 297, 341, 679, 971
DdeI CTNAG 4 cut(s) 521, 560, 1047, 1086
DrdI GACNNNNNNGTC 1 cut(s) 1139
DseDI GACNNNNNNGTC 1 cut(s) 1139
EaeI YGGCCR 2 cut(s) 813, 1243
Eam1104I CTCTTC 1 cut(s) 1301
EarI CTCTTC 1 cut(s) 1301
EciI GGCGGA 2 cut(s) 62, 1370
Ecl136II GAGCTC 1 cut(s) 1291
Eco24I GRGCYC 2 cut(s) 82, 1293
Eco47I GGWCC 2 cut(s) 719, 1080
Eco53kI GAGCTC 1 cut(s) 1291
Eco57I CTGAAG 1 cut(s) 1010
EcoICRI GAGCTC 1 cut(s) 1291
EcoRII CCWGG 2 cut(s) 507, 1245
EcoT38I GRGCYC 2 cut(s) 82, 1293
Esp3I CGTCTC 2 cut(s) 633, 1382
FalI AAGNNNNNCTT 4 cut(s) 472, 504, 983, 1015
FaqI GGGAC 1 cut(s) 74
FauI CCCGC 1 cut(s) 1048
FbaI TGATCA 3 cut(s) 166, 391, 432
FblI GTMKAC 2 cut(s) 985, 1143
Fnu4HI GCNGC 6 cut(s) 408, 1053, 1234, 1336, 1347, 1361
FokI GGATG 5 cut(s) 260, 451, 558, 841, 1324
FriOI GRGCYC 2 cut(s) 82, 1293
Fsp4HI GCNGC 6 cut(s) 408, 1053, 1234, 1336, 1347, 1361
FspBI CTAG 2 cut(s) 420, 501
FspI TGCGCA 1 cut(s) 228
GlaI GCGC 5 cut(s) 228, 428, 1111, 1232, 1298
GluI GCNGC 6 cut(s) 408, 1053, 1234, 1336, 1347, 1361
GsaI CCCAGC 1 cut(s) 473
GsuI CTGGAG 1 cut(s) 1277
HaeIII GGCC 2 cut(s) 815, 1245
HapII CCGG 1 cut(s) 1364
HgaI GACGC 1 cut(s) 380
HhaI GCGC 5 cut(s) 229, 429, 1112, 1233, 1299
Hin1I GRCGYC 1 cut(s) 1140
Hin6I GCGC 5 cut(s) 227, 427, 1110, 1231, 1297
HinP1I GCGC 5 cut(s) 227, 427, 1110, 1231, 1297
HincII GTYRAC 1 cut(s) 1144
HindII GTYRAC 1 cut(s) 1144
HindIII AAGCTT 2 cut(s) 734, 1265
HinfI GANTC 4 cut(s) 87, 332, 1009, 1226
HpaII CCGG 1 cut(s) 1364
HphI GGTGA 7 cut(s) 906, 942, 1006, 1113, 1163, 1174, 1315
Hpy166II GTNNAC 2 cut(s) 986, 1144
Hpy188I TCNGA 8 cut(s) 186, 258, 321, 391, 522, 638, 1048, 1128
Hpy188III TCNNGA 3 cut(s) 116, 436, 664
Hpy8I GTNNAC 2 cut(s) 986, 1144
Hpy99I CGWCG 7 cut(s) 258, 462, 1145, 1148, 1205, 1241, 1244
HpyAV CCTTC 9 cut(s) 204, 355, 396, 578, 1027, 1128, 1159, 1336, 1345
HpyCH4III ACNGT 3 cut(s) 195, 340, 631
HpyCH4IV ACGT 5 cut(s) 253, 377, 642, 781, 1140
HpyCH4V TGCA 6 cut(s) 29, 207, 535, 540, 593, 1171
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 44, 1242, 1273, 1352
HpyF3I CTNAG 4 cut(s) 521, 560, 1047, 1086
HpySE526I ACGT 5 cut(s) 253, 377, 642, 781, 1140
Hsp92I GRCGYC 1 cut(s) 1140
HspAI GCGC 5 cut(s) 227, 427, 1110, 1231, 1297
KroI GCCGGC 1 cut(s) 1363
KroNI GCCGGC 1 cut(s) 1365
Ksp22I TGATCA 3 cut(s) 166, 391, 432
LmnI GCTCC 5 cut(s) 478, 1281, 1288, 1296, 1357
Lsp1109I GCAGC 4 cut(s) 419, 1220, 1347, 1372
LweI GCATC 4 cut(s) 38, 194, 577, 602
MabI ACCWGGT 1 cut(s) 507
MaeI CTAG 2 cut(s) 420, 501
MaeII ACGT 5 cut(s) 253, 377, 642, 781, 1140
MaeIII GTNAC 6 cut(s) 171, 326, 503, 625, 643, 771
MfeI CAATTG 1 cut(s) 177
MflI RGATCY 1 cut(s) 1173
MhlI GDGCHC 3 cut(s) 82, 663, 1293
MlsI TGGCCA 1 cut(s) 815
MluCI AATT 1 cut(s) 177
MluNI TGGCCA 1 cut(s) 815
MlyI GAGTC 1 cut(s) 96
MmeI TCCRAC 1 cut(s) 236
Mox20I TGGCCA 1 cut(s) 815
MroNI GCCGGC 1 cut(s) 1363
MroXI GAANNNNTTC 1 cut(s) 737
MscI TGGCCA 1 cut(s) 815
MseI TTAA 2 cut(s) 479, 1005
MslI CAYNNNNRTG 1 cut(s) 108
Msp20I TGGCCA 1 cut(s) 815
MspI CCGG 1 cut(s) 1364
MspR9I CCNGG 2 cut(s) 509, 1247
MunI CAATTG 1 cut(s) 177
MvaI CCWGG 2 cut(s) 509, 1247
MvnI CGCG 3 cut(s) 665, 1231, 1299
MwoI GCNNNNNNNGC 5 cut(s) 35, 44, 1242, 1273, 1352
NaeI GCCGGC 1 cut(s) 1365
NgoMIV GCCGGC 1 cut(s) 1363
NlaIV GGNNCC 2 cut(s) 384, 1277
NmeAIII GCCGAG 2 cut(s) 101, 687
NmuCI GTSAC 3 cut(s) 326, 625, 643
NruI TCGCGA 1 cut(s) 665
NsbI TGCGCA 1 cut(s) 228
PagI TCATGA 2 cut(s) 115, 435
PcsI WCGNNNNNNNCGW 1 cut(s) 1140
PdiI GCCGGC 1 cut(s) 1365
PdmI GAANNNNTTC 1 cut(s) 737
PfeI GAWTC 3 cut(s) 332, 1009, 1226
PflMI CCANNNNNTGG 1 cut(s) 514
PkrI GCNGC 6 cut(s) 409, 1054, 1235, 1337, 1348, 1362
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
Psp124BI GAGCTC 1 cut(s) 1293
Psp1406I AACGTT 2 cut(s) 377, 781
Psp6I CCWGG 2 cut(s) 507, 1245
PspFI CCCAGC 1 cut(s) 469
PspGI CCWGG 2 cut(s) 507, 1245
PspN4I GGNNCC 2 cut(s) 384, 1277
PspPI GGNCC 2 cut(s) 719, 1080
PsrI GAACNNNNNNTAC 6 cut(s) 882, 914, 918, 950, 954, 986
PsuI RGATCY 1 cut(s) 1173
RruI TCGCGA 1 cut(s) 665
RsaI GTAC 5 cut(s) 197, 298, 342, 680, 972
RsaNI GTAC 5 cut(s) 196, 297, 341, 679, 971
RseI CAYNNNNRTG 1 cut(s) 108
SacI GAGCTC 1 cut(s) 1293
SalI GTCGAC 1 cut(s) 1142
SaqAI TTAA 2 cut(s) 479, 1005
SatI GCNGC 6 cut(s) 408, 1053, 1234, 1336, 1347, 1361
Sau96I GGNCC 2 cut(s) 719, 1080
ScaI AGTACT 1 cut(s) 298
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 2 cut(s) 509, 1247
SduI GDGCHC 3 cut(s) 82, 663, 1293
SexAI ACCWGGT 1 cut(s) 507
SfaNI GCATC 4 cut(s) 38, 194, 577, 602
SgrDI CGTCGACG 1 cut(s) 1142
SinI GGWCC 2 cut(s) 719, 1080
SmiMI CAYNNNNRTG 1 cut(s) 108
Sse9I AATT 1 cut(s) 177
SsiI CCGC 8 cut(s) 73, 1052, 1055, 1115, 1149, 1195, 1346, 1355
SspMI CTAG 2 cut(s) 420, 501
SstI GAGCTC 1 cut(s) 1293
StyD4I CCNGG 2 cut(s) 507, 1245
TaaI ACNGT 3 cut(s) 195, 340, 631
TaiI ACGT 5 cut(s) 256, 380, 645, 784, 1143
TaqI TCGA 9 cut(s) 121, 351, 362, 443, 789, 870, 1143, 1260, 1284
TasI AATT 1 cut(s) 177
TatI WGTACW 3 cut(s) 296, 678, 970
TauI GCSGC 2 cut(s) 1055, 1349
TfiI GAWTC 3 cut(s) 332, 1009, 1226
Tru1I TTAA 2 cut(s) 479, 1005
Tru9I TTAA 2 cut(s) 479, 1005
TscAI CASTG 6 cut(s) 198, 537, 816, 891, 1090, 1106
TseFI GTSAC 3 cut(s) 326, 625, 643
TseI GCWGC 4 cut(s) 407, 1233, 1335, 1360
Tsp45I GTSAC 3 cut(s) 326, 625, 643
TspDTI ATGAA 7 cut(s) 17, 104, 150, 337, 757, 795, 1009
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 6 cut(s) 198, 537, 816, 891, 1090, 1106
Van91I CCANNNNNTGG 1 cut(s) 514
VpaK11BI GGWCC 2 cut(s) 719, 1080
XcmI CCANNNNNNNNNTGG 1 cut(s) 49
XmiI GTMKAC 2 cut(s) 985, 1143
XmnI GAANNNNTTC 1 cut(s) 737
XspI CTAG 2 cut(s) 420, 501
ZraI GACGTC 1 cut(s) 1141
ZrmI AGTACT 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.