Rroxscaffold_1G00018120

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0009518
Physical Location & Seq
Reverse (-)
22262746 .. 22265875
3130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00018120.1

Sequence Viewer

Length: 750 bp
ATGAAAGAAATGGATCGTTTCTTCTGTGCATCTTCTGCTTCCACAGCCATCATGGATCAAAGAGCCATAGTCCGCCGAGCCCACAGAGTCCCCATTAGCGACCATGTTTATGTTCATGATCGAAGAAAAAACCAACCCCATGTTCCTATGATCCCTTGTTCATTTGATCAGTTACCAATTGATCCGAAACCACTATACGAAAGATGCAGAAGGAGTTTTTCTTCTGCGCAGAGTAGCGATAGAGATCAGCTACGTCGGAAAAGCTCTGCTGACATCCATGAGCAAAGAAGAAAGAGTACTTCCTCCACTTCTCGGTATGGGATTAGTGACGATTCCACAGTACCCTTCATCGACTGGTTATCTGAAACTGACGCAACGTTGGTTCCTTCTGATCATCATAAAGAAGCAGCATCTAACCCTAGACTGCGGCTGATCATGAGATCGAATGATGAATATCGTCGTCCATCCCCAGCTCCATTAAGGTCGTCTTCCACTCGCTCTCGTAACCAGGTTGTGGTTATGAGGGTGTCAGTGCATTGCAAGGGATGTGAAGAAAAACTGAGAAAGCATCTCTCTAAAATGGAAGGAGTTGCATCATTTAGTATAGATTTACCAACAAAGAAAGTCACAGTCATCGGAGACGTGACACCATTAGGTGTGCTCGCGAGTGTATCCAAGGTGAAGAAAGCTCAGCTCTGGTCGTCTCCATCATTTTCATCACCTTCACCATCTTCTCGATGGTCAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

249

Amino Acids

28.15

Weight (kDa)

10.06

Isoelectric Point (pI)

66.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 174 - 220 8.3e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 228
AccB7I CCANNNNNTGG 1 cut(s) 514
AccII CGCG 1 cut(s) 665
AciI CCGC 2 cut(s) 73, 427
AclI AACGTT 1 cut(s) 377
AclWI GGATC 4 cut(s) 21, 63, 145, 176
AfaI GTAC 2 cut(s) 298, 342
AfiI CCNNNNNNNGG 2 cut(s) 312, 514
AjiI CACGTC 1 cut(s) 643
AjnI CCWGG 1 cut(s) 507
AjuI GAANNNNNNNTTGG 2 cut(s) 126, 158
AluBI AGCT 5 cut(s) 250, 264, 473, 689, 694
AluI AGCT 5 cut(s) 250, 264, 473, 689, 694
Alw21I GWGCWC 1 cut(s) 663
Alw26I GTCTC 2 cut(s) 633, 708
AlwI GGATC 4 cut(s) 21, 63, 145, 176
ApeKI GCWGC 1 cut(s) 407
AspLEI GCGC 1 cut(s) 229
AsuHPI GGTGA 3 cut(s) 691, 711, 717
BanII GRGCYC 1 cut(s) 82
BarI GAAGNNNNNNTAC 2 cut(s) 280, 312
BbsI GAAGAC 1 cut(s) 480
Bbv12I GWGCWC 1 cut(s) 663
BbvI GCAGC 1 cut(s) 419
BccI CCATC 5 cut(s) 56, 472, 715, 732, 736
BciT130I CCWGG 1 cut(s) 509
BciVI GTATCC 1 cut(s) 682
BclI TGATCA 3 cut(s) 166, 391, 432
BcoDI GTCTC 2 cut(s) 633, 708
BfaI CTAG 1 cut(s) 420
BfuI GTATCC 1 cut(s) 682
BisI GCNGC 2 cut(s) 408, 428
BlpI GCTNAGC 1 cut(s) 690
BlsI GCNGC 2 cut(s) 409, 429
BmcAI AGTACT 1 cut(s) 298
Bme1390I CCNGG 1 cut(s) 509
BmgBI CACGTC 1 cut(s) 643
BmiI GGNNCC 1 cut(s) 384
BmrFI CCNGG 1 cut(s) 509
BmsI GCATC 5 cut(s) 38, 194, 419, 577, 602
BpiI GAAGAC 1 cut(s) 480
Bpu1102I GCTNAGC 1 cut(s) 690
BsaBI GATNNNNATC 2 cut(s) 243, 453
BsaJI CCNNGG 1 cut(s) 675
Bsc4I CCNNNNNNNGG 2 cut(s) 312, 514
Bse1I ACTGG 1 cut(s) 359
Bse3DI GCAATG 1 cut(s) 535
Bse8I GATNNNNATC 2 cut(s) 243, 453
BseBI CCWGG 1 cut(s) 509
BseDI CCNNGG 1 cut(s) 675
BseGI GGATG 3 cut(s) 273, 464, 551
BseJI GATNNNNATC 2 cut(s) 243, 453
BseLI CCNNNNNNNGG 2 cut(s) 312, 514
BseMI GCAATG 1 cut(s) 535
BseMII CTCAG 2 cut(s) 551, 704
BseNI ACTGG 1 cut(s) 359
BseXI GCAGC 1 cut(s) 419
BseYI CCCAGC 1 cut(s) 469
Bsh1236I CGCG 1 cut(s) 665
BsiHKAI GWGCWC 1 cut(s) 663
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 2 cut(s) 312, 514
BsmAI GTCTC 2 cut(s) 633, 708
BsmBI CGTCTC 2 cut(s) 633, 708
BsmFI GGGAC 1 cut(s) 74
Bsp1286I GDGCHC 2 cut(s) 82, 663
Bsp1720I GCTNAGC 1 cut(s) 690
Bsp68I TCGCGA 1 cut(s) 665
BspACI CCGC 2 cut(s) 73, 427
BspCNI CTCAG 2 cut(s) 552, 703
BspFNI CGCG 1 cut(s) 665
BspHI TCATGA 2 cut(s) 115, 435
BspLI GGNNCC 1 cut(s) 384
BspPI GGATC 4 cut(s) 21, 63, 145, 176
BsrDI GCAATG 1 cut(s) 535
BsrI ACTGG 1 cut(s) 359
BssECI CCNNGG 1 cut(s) 675
BssT1I CCWWGG 1 cut(s) 675
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 2 cut(s) 340, 631
BstAPI GCANNNNNTGC 1 cut(s) 35
BstC8I GCNNGC 1 cut(s) 663
BstDEI CTNAG 2 cut(s) 560, 690
BstF5I GGATG 3 cut(s) 273, 464, 551
BstFNI CGCG 1 cut(s) 665
BstHHI GCGC 1 cut(s) 229
BstMAI GTCTC 2 cut(s) 633, 708
BstMWI GCNNNNNNNGC 2 cut(s) 35, 44
BstNI CCWGG 1 cut(s) 509
BstSCI CCNGG 1 cut(s) 507
BstUI CGCG 1 cut(s) 665
BstV1I GCAGC 1 cut(s) 419
BstV2I GAAGAC 1 cut(s) 480
BsuI GTATCC 1 cut(s) 682
BtrI CACGTC 1 cut(s) 643
BtsCI GGATG 3 cut(s) 273, 464, 551
BtsIMutI CAGTG 1 cut(s) 537
BtuMI TCGCGA 1 cut(s) 665
Cac8I GCNNGC 1 cut(s) 663
CciI TCATGA 2 cut(s) 115, 435
CfoI GCGC 1 cut(s) 229
CseI GACGC 1 cut(s) 380
CsiI ACCWGGT 1 cut(s) 507
Csp6I GTAC 2 cut(s) 297, 341
CviAII CATG 7 cut(s) 52, 104, 116, 140, 278, 436, 747
CviJI RGCY 9 cut(s) 47, 65, 80, 250, 264, 430, 473, 689, 694
CviKI_1 RGCY 9 cut(s) 47, 65, 80, 250, 264, 430, 473, 689, 694
CviQI GTAC 2 cut(s) 297, 341
DdeI CTNAG 2 cut(s) 560, 690
EciI GGCGGA 1 cut(s) 62
Eco130I CCWWGG 1 cut(s) 675
Eco24I GRGCYC 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 507
EcoT14I CCWWGG 1 cut(s) 675
EcoT38I GRGCYC 1 cut(s) 82
ErhI CCWWGG 1 cut(s) 675
Esp3I CGTCTC 2 cut(s) 633, 708
FaeI CATG 7 cut(s) 55, 107, 119, 143, 281, 439, 750
FalI AAGNNNNNCTT 2 cut(s) 472, 504
FaqI GGGAC 1 cut(s) 74
FatI CATG 7 cut(s) 51, 103, 115, 139, 277, 435, 746
FbaI TGATCA 3 cut(s) 166, 391, 432
Fnu4HI GCNGC 2 cut(s) 408, 428
FokI GGATG 3 cut(s) 260, 451, 558
FriOI GRGCYC 1 cut(s) 82
Fsp4HI GCNGC 2 cut(s) 408, 428
FspBI CTAG 1 cut(s) 420
FspI TGCGCA 1 cut(s) 228
GlaI GCGC 1 cut(s) 228
GluI GCNGC 2 cut(s) 408, 428
GsaI CCCAGC 1 cut(s) 473
HgaI GACGC 1 cut(s) 380
HhaI GCGC 1 cut(s) 229
Hin1II CATG 7 cut(s) 55, 107, 119, 143, 281, 439, 750
Hin6I GCGC 1 cut(s) 227
HinP1I GCGC 1 cut(s) 227
HinfI GANTC 2 cut(s) 87, 332
HphI GGTGA 3 cut(s) 691, 711, 717
Hpy188I TCNGA 5 cut(s) 186, 258, 364, 391, 638
Hpy188III TCNNGA 4 cut(s) 116, 436, 664, 735
Hpy99I CGWCG 2 cut(s) 258, 462
HpyAV CCTTC 5 cut(s) 204, 355, 396, 578, 732
HpyCH4III ACNGT 2 cut(s) 340, 631
HpyCH4IV ACGT 3 cut(s) 253, 377, 642
HpyCH4V TGCA 5 cut(s) 29, 207, 535, 540, 593
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 44
HpyF3I CTNAG 2 cut(s) 560, 690
HpySE526I ACGT 3 cut(s) 253, 377, 642
Hsp92II CATG 7 cut(s) 55, 107, 119, 143, 281, 439, 750
HspAI GCGC 1 cut(s) 227
Ksp22I TGATCA 3 cut(s) 166, 391, 432
LmnI GCTCC 1 cut(s) 478
LpnPI CCDG 5 cut(s) 340, 483, 494, 521, 682
Lsp1109I GCAGC 1 cut(s) 419
LweI GCATC 5 cut(s) 38, 194, 419, 577, 602
MabI ACCWGGT 1 cut(s) 507
MaeI CTAG 1 cut(s) 420
MaeII ACGT 3 cut(s) 253, 377, 642
MaeIII GTNAC 5 cut(s) 171, 326, 503, 625, 643
MboII GAAGA 9 cut(s) 13, 24, 135, 213, 300, 480, 563, 694, 723
MfeI CAATTG 1 cut(s) 177
MhlI GDGCHC 2 cut(s) 82, 663
MluCI AATT 1 cut(s) 177
MlyI GAGTC 1 cut(s) 96
MmeI TCCRAC 1 cut(s) 236
MnlI CCTC 2 cut(s) 313, 516
MseI TTAA 1 cut(s) 479
MslI CAYNNNNRTG 1 cut(s) 108
MspR9I CCNGG 1 cut(s) 509
MunI CAATTG 1 cut(s) 177
MvaI CCWGG 1 cut(s) 509
MvnI CGCG 1 cut(s) 665
MwoI GCNNNNNNNGC 2 cut(s) 35, 44
NlaIII CATG 7 cut(s) 55, 107, 119, 143, 281, 439, 750
NlaIV GGNNCC 1 cut(s) 384
NmeAIII GCCGAG 1 cut(s) 101
NmuCI GTSAC 3 cut(s) 326, 625, 643
NruI TCGCGA 1 cut(s) 665
NsbI TGCGCA 1 cut(s) 228
PagI TCATGA 2 cut(s) 115, 435
PfeI GAWTC 1 cut(s) 332
PflMI CCANNNNNTGG 1 cut(s) 514
PkrI GCNGC 2 cut(s) 409, 429
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
Psp1406I AACGTT 1 cut(s) 377
Psp6I CCWGG 1 cut(s) 507
PspFI CCCAGC 1 cut(s) 469
PspGI CCWGG 1 cut(s) 507
PspN4I GGNNCC 1 cut(s) 384
RruI TCGCGA 1 cut(s) 665
RsaI GTAC 2 cut(s) 298, 342
RsaNI GTAC 2 cut(s) 297, 341
RseI CAYNNNNRTG 1 cut(s) 108
SaqAI TTAA 1 cut(s) 479
SatI GCNGC 2 cut(s) 408, 428
ScaI AGTACT 1 cut(s) 298
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 1 cut(s) 509
SduI GDGCHC 2 cut(s) 82, 663
SexAI ACCWGGT 1 cut(s) 507
SfaNI GCATC 5 cut(s) 38, 194, 419, 577, 602
SmiMI CAYNNNNRTG 1 cut(s) 108
Sse9I AATT 1 cut(s) 177
SsiI CCGC 2 cut(s) 73, 427
SspMI CTAG 1 cut(s) 420
StyD4I CCNGG 1 cut(s) 507
StyI CCWWGG 1 cut(s) 675
TaaI ACNGT 2 cut(s) 340, 631
TaiI ACGT 3 cut(s) 256, 380, 645
TaqI TCGA 4 cut(s) 121, 351, 443, 736
TasI AATT 1 cut(s) 177
TatI WGTACW 1 cut(s) 296
TauI GCSGC 1 cut(s) 430
TfiI GAWTC 1 cut(s) 332
Tru1I TTAA 1 cut(s) 479
Tru9I TTAA 1 cut(s) 479
TscAI CASTG 1 cut(s) 537
TseFI GTSAC 3 cut(s) 326, 625, 643
TseI GCWGC 1 cut(s) 407
Tsp45I GTSAC 3 cut(s) 326, 625, 643
TspDTI ATGAA 6 cut(s) 17, 104, 150, 337, 465, 705
TspRI CASTG 1 cut(s) 537
Van91I CCANNNNNTGG 1 cut(s) 514
XcmI CCANNNNNNNNNTGG 2 cut(s) 49, 735
XspI CTAG 1 cut(s) 420
ZrmI AGTACT 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.