MD11G1115900.v1.1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0009518
Physical Location & Seq
Reverse (-)
10466133 .. 10467748
1616 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1115900.v1.1.491

Sequence Viewer

Length: 831 bp
ATGAAAGGAATGGATATTTTCTGTGCATCTTCAGCTTCCACAGCCATTTGCTCTAGCCTGGATGCCTTGGTCCGCCGAGGCCCCAGACCCCTTGATCATGGTCCTTATCATCGTCTTCGTGATCACGATCAACGGAAAAACCAACCTCATCATCAAACTCACCATGCCCCCTGCTCATCTCAGTTACCTATTGATCCCAAACCATTTTACGAAATATGTAGAAAGAGTTTTTCTAGTGCACAAAGGCAGCTACGTACAAAAAGCTCTGCTGAGATACATGACTTAACAAGGACTAAGAGTACCAATGGCTCTTCTTCTTCTTCTCGGTTCCTCTCAAGTGACTCGCCCTTCATTGACAGGATAACGGAGCCCGATTGTAAGAATGTCTCTGCAGCTGCATTTGTTCCTCCTCAAGTACCCTCTAAGCCTAAAGACTCGATGAGCTCAAATGTTCCTCCTCAAGTACCTTCTAAGCCTAGAGTCTCGACGAGCTCAAATGTTCCTCCTCAAGTACCCTCTAAGCCTAGAAGGTCGATGAGCTCAAATGGTCATTGTAGTACCGTTTTGAAGTCTTCTTCTACTAGTTCTCGTCACCAGGTTGTTGTTTTGAGGGTGTCACTGCATTGCAAGGGTTGTGAAGGAAAAGTGAGAAAACACCTCTCTAAAATGGAAGGAGTGACTTCATTTAGCATAGATTTCCAAACAAAGAGAGTCACAGTGGTGGGGGATGTCACCCCATTAGGTGTGCTCTCAAGTGTATCCAAGGTGAGGAAATCCCAGCTCTGGCCTTCTCCAACATCACCATCACCATCCTCTCGGTTGCCAGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

277

Amino Acids

30.11

Weight (kDa)

10.22

Isoelectric Point (pI)

62.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 203 - 249 2.8e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 73
AclWI GGATC 1 cut(s) 188
AcuI CTGAAG 1 cut(s) 15
AfaI GTAC 6 cut(s) 256, 301, 417, 465, 513, 559
AfiI CCNNNNNNNGG 2 cut(s) 768, 783
AgsI TTSAA 1 cut(s) 568
AhlI ACTAGT 1 cut(s) 581
AjnI CCWGG 2 cut(s) 57, 594
AleI CACNNNNGTG 1 cut(s) 719
AluBI AGCT 8 cut(s) 35, 250, 264, 395, 444, 492, 540, 781
AluI AGCT 8 cut(s) 35, 250, 264, 395, 444, 492, 540, 781
Alw21I GWGCWC 5 cut(s) 241, 446, 494, 542, 750
Alw26I GTCTC 2 cut(s) 391, 487
Alw44I GTGCAC 1 cut(s) 237
AlwI GGATC 1 cut(s) 188
AoxI GGCC 2 cut(s) 79, 785
ApaLI GTGCAC 1 cut(s) 237
ApeKI GCWGC 3 cut(s) 247, 392, 395
AspS9I GGNCC 3 cut(s) 70, 80, 101
AsuHPI GGTGA 6 cut(s) 152, 584, 724, 778, 792, 798
AvaII GGWCC 2 cut(s) 70, 101
BaeGI GKGCMC 1 cut(s) 241
BanII GRGCYC 4 cut(s) 372, 446, 494, 542
BbsI GAAGAC 2 cut(s) 107, 564
Bbv12I GWGCWC 5 cut(s) 241, 446, 494, 542, 750
BbvI GCAGC 3 cut(s) 259, 382, 404
BccI CCATC 2 cut(s) 811, 817
BciT130I CCWGG 2 cut(s) 59, 596
BciVI GTATCC 1 cut(s) 769
BclI TGATCA 2 cut(s) 94, 121
BcoDI GTCTC 2 cut(s) 391, 487
BcuI ACTAGT 1 cut(s) 581
BfaI CTAG 5 cut(s) 54, 234, 477, 525, 582
BfmI CTRYAG 1 cut(s) 390
BfuI GTATCC 1 cut(s) 769
BisI GCNGC 3 cut(s) 248, 393, 396
BlsI GCNGC 3 cut(s) 249, 394, 397
Bme1390I CCNGG 2 cut(s) 59, 596
Bme18I GGWCC 2 cut(s) 70, 101
BmgT120I GGNCC 3 cut(s) 70, 80, 101
BmiI GGNNCC 3 cut(s) 82, 329, 369
BmrFI CCNGG 2 cut(s) 59, 596
BmsI GCATC 2 cut(s) 35, 52
BpiI GAAGAC 2 cut(s) 107, 564
BpuEI CTTGAG 5 cut(s) 319, 396, 444, 492, 736
BsaAI YACGTR 1 cut(s) 254
BsaBI GATNNNNATC 1 cut(s) 126
BsaJI CCNNGG 3 cut(s) 66, 76, 762
Bsc4I CCNNNNNNNGG 2 cut(s) 768, 783
Bse3DI GCAATG 1 cut(s) 622
Bse8I GATNNNNATC 1 cut(s) 126
BseBI CCWGG 2 cut(s) 59, 596
BseDI CCNNGG 3 cut(s) 66, 76, 762
BseGI GGATG 3 cut(s) 67, 733, 809
BseJI GATNNNNATC 1 cut(s) 126
BseLI CCNNNNNNNGG 2 cut(s) 768, 783
BseMI GCAATG 1 cut(s) 622
BseMII CTCAG 2 cut(s) 194, 261
BseRI GAGGAG 3 cut(s) 399, 447, 495
BseSI GKGCMC 1 cut(s) 241
BseXI GCAGC 3 cut(s) 259, 382, 404
BseYI CCCAGC 1 cut(s) 777
BshFI GGCC 2 cut(s) 81, 787
BsiHKAI GWGCWC 5 cut(s) 241, 446, 494, 542, 750
BslI CCNNNNNNNGG 2 cut(s) 768, 783
BsmAI GTCTC 2 cut(s) 391, 487
BsnI GGCC 2 cut(s) 81, 787
Bsp1286I GDGCHC 6 cut(s) 241, 372, 446, 494, 542, 750
Bsp143I GATC 4 cut(s) 94, 121, 127, 193
BspACI CCGC 1 cut(s) 73
BspANI GGCC 2 cut(s) 81, 787
BspCNI CTCAG 2 cut(s) 193, 262
BspLI GGNNCC 3 cut(s) 82, 329, 369
BspMAI CTGCAG 1 cut(s) 394
BspPI GGATC 1 cut(s) 188
BspQI GCTCTTC 1 cut(s) 316
BsrDI GCAATG 1 cut(s) 622
BssECI CCNNGG 3 cut(s) 66, 76, 762
BssMI GATC 4 cut(s) 94, 121, 127, 193
BssT1I CCWWGG 2 cut(s) 66, 762
Bst2UI CCWGG 2 cut(s) 59, 596
Bst4CI ACNGT 2 cut(s) 562, 718
Bst6I CTCTTC 1 cut(s) 316
BstBAI YACGTR 1 cut(s) 254
BstC8I GCNNGC 1 cut(s) 825
BstDEI CTNAG 6 cut(s) 180, 270, 294, 423, 471, 519
BstF5I GGATG 3 cut(s) 67, 733, 809
BstKTI GATC 4 cut(s) 97, 124, 130, 196
BstMAI GTCTC 2 cut(s) 391, 487
BstMBI GATC 4 cut(s) 94, 121, 127, 193
BstMWI GCNNNNNNNGC 2 cut(s) 32, 41
BstNI CCWGG 2 cut(s) 59, 596
BstSCI CCNGG 2 cut(s) 57, 594
BstSFI CTRYAG 1 cut(s) 390
BstSLI GKGCMC 1 cut(s) 241
BstSNI TACGTA 1 cut(s) 254
BstV1I GCAGC 3 cut(s) 259, 382, 404
BstV2I GAAGAC 2 cut(s) 107, 564
BsuI GTATCC 1 cut(s) 769
BsuRI GGCC 2 cut(s) 81, 787
BtsCI GGATG 3 cut(s) 67, 733, 809
BtsI GCAGTG 1 cut(s) 617
BtsIMutI CAGTG 2 cut(s) 617, 723
Cac8I GCNNGC 1 cut(s) 825
Cfr13I GGNCC 3 cut(s) 70, 80, 101
CsiI ACCWGGT 1 cut(s) 594
Csp6I GTAC 6 cut(s) 255, 300, 416, 464, 512, 558
CviAII CATG 3 cut(s) 98, 164, 278
CviQI GTAC 6 cut(s) 255, 300, 416, 464, 512, 558
DdeI CTNAG 6 cut(s) 180, 270, 294, 423, 471, 519
DpnI GATC 4 cut(s) 96, 123, 129, 195
DpnII GATC 4 cut(s) 94, 121, 127, 193
Eam1104I CTCTTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 316
EciI GGCGGA 1 cut(s) 62
Ecl136II GAGCTC 3 cut(s) 444, 492, 540
Eco105I TACGTA 1 cut(s) 254
Eco130I CCWWGG 2 cut(s) 66, 762
Eco24I GRGCYC 4 cut(s) 372, 446, 494, 542
Eco47I GGWCC 2 cut(s) 70, 101
Eco53kI GAGCTC 3 cut(s) 444, 492, 540
Eco57I CTGAAG 1 cut(s) 15
EcoICRI GAGCTC 3 cut(s) 444, 492, 540
EcoO109I RGGNCCY 1 cut(s) 80
EcoRII CCWGG 2 cut(s) 57, 594
EcoT14I CCWWGG 2 cut(s) 66, 762
EcoT38I GRGCYC 4 cut(s) 372, 446, 494, 542
ErhI CCWWGG 2 cut(s) 66, 762
FaeI CATG 3 cut(s) 101, 167, 281
FaiI YATR 5 cut(s) 99, 165, 217, 279, 692
FatI CATG 3 cut(s) 97, 163, 277
FbaI TGATCA 2 cut(s) 94, 121
Fnu4HI GCNGC 3 cut(s) 248, 393, 396
FokI GGATG 3 cut(s) 74, 740, 796
FriOI GRGCYC 4 cut(s) 372, 446, 494, 542
Fsp4HI GCNGC 3 cut(s) 248, 393, 396
FspBI CTAG 5 cut(s) 54, 234, 477, 525, 582
GluI GCNGC 3 cut(s) 248, 393, 396
GsaI CCCAGC 1 cut(s) 781
HaeIII GGCC 2 cut(s) 81, 787
Hin1II CATG 3 cut(s) 101, 167, 281
HinfI GANTC 4 cut(s) 341, 434, 480, 711
HphI GGTGA 6 cut(s) 152, 584, 724, 778, 792, 798
Hpy166II GTNNAC 1 cut(s) 239
Hpy188III TCNNGA 3 cut(s) 119, 125, 484
Hpy8I GTNNAC 1 cut(s) 239
Hpy99I CGWCG 1 cut(s) 490
HpyAV CCTTC 6 cut(s) 358, 477, 522, 632, 665, 798
HpyCH4III ACNGT 2 cut(s) 562, 718
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 6 cut(s) 26, 239, 392, 398, 622, 627
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 41
HpyF3I CTNAG 6 cut(s) 180, 270, 294, 423, 471, 519
HpySE526I ACGT 1 cut(s) 253
Hsp92II CATG 3 cut(s) 101, 167, 281
Ksp22I TGATCA 2 cut(s) 94, 121
Kzo9I GATC 4 cut(s) 94, 121, 127, 193
LguI GCTCTTC 1 cut(s) 316
LmnI GCTCC 1 cut(s) 367
LpnPI CCDG 9 cut(s) 44, 71, 97, 184, 343, 581, 608, 769, 791
Lsp1109I GCAGC 3 cut(s) 259, 382, 404
LweI GCATC 2 cut(s) 35, 52
MabI ACCWGGT 1 cut(s) 594
MaeI CTAG 5 cut(s) 54, 234, 477, 525, 582
MaeII ACGT 1 cut(s) 253
MaeIII GTNAC 7 cut(s) 183, 338, 590, 615, 676, 712, 730
MalI GATC 4 cut(s) 96, 123, 129, 195
MboI GATC 4 cut(s) 94, 121, 127, 193
MboII GAAGA 8 cut(s) 21, 107, 303, 306, 309, 312, 564, 567
MhlI GDGCHC 6 cut(s) 241, 372, 446, 494, 542, 750
MlyI GAGTC 4 cut(s) 335, 428, 489, 720
MmeI TCCRAC 1 cut(s) 818
MseI TTAA 1 cut(s) 284
MslI CAYNNNNRTG 1 cut(s) 719
MspA1I CMGCKG 1 cut(s) 395
MspR9I CCNGG 2 cut(s) 59, 596
MvaI CCWGG 2 cut(s) 59, 596
MwoI GCNNNNNNNGC 2 cut(s) 32, 41
NdeII GATC 4 cut(s) 94, 121, 127, 193
NlaIII CATG 3 cut(s) 101, 167, 281
NlaIV GGNNCC 3 cut(s) 82, 329, 369
NmeAIII GCCGAG 1 cut(s) 101
NmuCI GTSAC 6 cut(s) 338, 590, 615, 676, 712, 730
OliI CACNNNNGTG 1 cut(s) 719
PciSI GCTCTTC 1 cut(s) 316
PkrI GCNGC 3 cut(s) 249, 394, 397
PleI GAGTC 4 cut(s) 335, 428, 488, 719
PpsI GAGTC 4 cut(s) 335, 428, 488, 719
Ppu21I YACGTR 1 cut(s) 254
Psp124BI GAGCTC 3 cut(s) 446, 494, 542
Psp6I CCWGG 2 cut(s) 57, 594
PspFI CCCAGC 1 cut(s) 777
PspGI CCWGG 2 cut(s) 57, 594
PspN4I GGNNCC 3 cut(s) 82, 329, 369
PspPI GGNCC 3 cut(s) 70, 80, 101
PstI CTGCAG 1 cut(s) 394
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 6 cut(s) 256, 301, 417, 465, 513, 559
RsaNI GTAC 6 cut(s) 255, 300, 416, 464, 512, 558
RseI CAYNNNNRTG 1 cut(s) 719
SacI GAGCTC 3 cut(s) 446, 494, 542
SapI GCTCTTC 1 cut(s) 316
SaqAI TTAA 1 cut(s) 284
SatI GCNGC 3 cut(s) 248, 393, 396
Sau3AI GATC 4 cut(s) 94, 121, 127, 193
Sau96I GGNCC 3 cut(s) 70, 80, 101
SchI GAGTC 4 cut(s) 335, 428, 489, 720
ScrFI CCNGG 2 cut(s) 59, 596
SduI GDGCHC 6 cut(s) 241, 372, 446, 494, 542, 750
SexAI ACCWGGT 1 cut(s) 594
SfaNI GCATC 2 cut(s) 35, 52
SfcI CTRYAG 1 cut(s) 390
SinI GGWCC 2 cut(s) 70, 101
SmiMI CAYNNNNRTG 1 cut(s) 719
SmlI CTYRAG 5 cut(s) 334, 411, 459, 507, 751
SmoI CTYRAG 5 cut(s) 334, 411, 459, 507, 751
SnaBI TACGTA 1 cut(s) 254
SpeI ACTAGT 1 cut(s) 581
SsiI CCGC 1 cut(s) 73
SspMI CTAG 5 cut(s) 54, 234, 477, 525, 582
SstI GAGCTC 3 cut(s) 446, 494, 542
StyD4I CCNGG 2 cut(s) 57, 594
StyI CCWWGG 2 cut(s) 66, 762
TaaI ACNGT 2 cut(s) 562, 718
TaiI ACGT 1 cut(s) 256
TaqI TCGA 3 cut(s) 437, 485, 533
Tru1I TTAA 1 cut(s) 284
Tru9I TTAA 1 cut(s) 284
TscAI CASTG 2 cut(s) 624, 723
TseFI GTSAC 6 cut(s) 338, 590, 615, 676, 712, 730
TseI GCWGC 3 cut(s) 247, 392, 395
Tsp45I GTSAC 6 cut(s) 338, 590, 615, 676, 712, 730
TspDTI ATGAA 3 cut(s) 17, 340, 672
TspGWI ACGGA 2 cut(s) 148, 380
TspRI CASTG 2 cut(s) 624, 723
VneI GTGCAC 1 cut(s) 237
VpaK11BI GGWCC 2 cut(s) 70, 101
XspI CTAG 5 cut(s) 54, 234, 477, 525, 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.