RchiOBHm_Chr5g0062481

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0009518
Physical Location & Seq
Forward (+)
68066402 .. 68068986
2585 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33873

Sequence Viewer

Length: 741 bp
ATGGATCGTTTCTTCTGTGCATCTTCTGCTTCCACAGCCATCATGGATCAAAGAGCCATAGTCCGCCGAGCCCACAGAGTCCCCATTAGCGACCATGTTTATGTTCATGGTCGAAGAAAAAACCAACCCCATCTTCCTATGATCCCTTGTTCATTTGACCAGTTACCAATTGATCCGAAACCACTGTACGAAAGATGCAGAAGGAGTTTTTCTTCTGCGCAGTGTAGCGATAGAGATCAGCTACGTCGGAAAAGCTCTGCTGACATCCATGATCAAAGAAGAAAGAGTACTTCCTCCACTTCTCGGTATCGGATTAGTGACGGTTCCACAGTACCCTTCATCGACTGGTTATCGGAAACTGACGCAACGTTGGTTCCTTCTGATCATCATAAAGAAGCAGCATCTAACCCTAGACTGCGCCTGATCATGAGATCGAATGATGAATATCGTCGTCCATCCCCAGCTCCATTAAGGTCGTCTTCCACTCGCTCTCGTAACCAGGTTGTGGTTCTGAGGGTGTCAGTGCATTGCAAGGGATGTGAAGAAAAACTGAGAAAGCATCTCTCTAAAATGGAAGGAGTTGCATCATTTAGTATAGATTTACCAACAAAGAAAGTCACAGTCATCGGAGACGTGACACCATTAGGTGTGCTCGCGAGTGTATCCAAGGTGAAGAAAGCTCAGCTCTGGTCGTCTCCAACATCTTCATCACCTTCACCATCTTCTCGATGGTCAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

246

Amino Acids

27.69

Weight (kDa)

10.28

Isoelectric Point (pI)

64.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 171 - 217 5.5e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 219
AccB7I CCANNNNNTGG 1 cut(s) 505
AccII CGCG 1 cut(s) 656
AciI CCGC 1 cut(s) 64
AclI AACGTT 1 cut(s) 368
AclWI GGATC 4 cut(s) 12, 54, 136, 167
AfaI GTAC 3 cut(s) 188, 289, 333
AfiI CCNNNNNNNGG 2 cut(s) 303, 505
AjiI CACGTC 1 cut(s) 634
AjnI CCWGG 1 cut(s) 498
AjuI GAANNNNNNNTTGG 2 cut(s) 117, 149
AluBI AGCT 5 cut(s) 241, 255, 464, 680, 685
AluI AGCT 5 cut(s) 241, 255, 464, 680, 685
Alw21I GWGCWC 1 cut(s) 654
Alw26I GTCTC 2 cut(s) 624, 699
AlwI GGATC 4 cut(s) 12, 54, 136, 167
ApeKI GCWGC 1 cut(s) 398
AspLEI GCGC 2 cut(s) 220, 420
AsuHPI GGTGA 3 cut(s) 682, 702, 708
BanII GRGCYC 1 cut(s) 73
BarI GAAGNNNNNNTAC 2 cut(s) 271, 303
BbsI GAAGAC 1 cut(s) 471
Bbv12I GWGCWC 1 cut(s) 654
BbvI GCAGC 1 cut(s) 410
BccI CCATC 5 cut(s) 47, 138, 463, 723, 727
BciT130I CCWGG 1 cut(s) 500
BciVI GTATCC 1 cut(s) 673
BclI TGATCA 3 cut(s) 271, 382, 423
BcoDI GTCTC 2 cut(s) 624, 699
BfaI CTAG 1 cut(s) 411
BfuI GTATCC 1 cut(s) 673
BisI GCNGC 1 cut(s) 399
BlpI GCTNAGC 1 cut(s) 681
BlsI GCNGC 1 cut(s) 400
BmcAI AGTACT 1 cut(s) 289
Bme1390I CCNGG 1 cut(s) 500
BmgBI CACGTC 1 cut(s) 634
BmiI GGNNCC 2 cut(s) 325, 375
BmrFI CCNGG 1 cut(s) 500
BmsI GCATC 5 cut(s) 29, 185, 410, 568, 593
BpiI GAAGAC 1 cut(s) 471
Bpu1102I GCTNAGC 1 cut(s) 681
BsaBI GATNNNNATC 2 cut(s) 234, 444
BsaJI CCNNGG 1 cut(s) 666
Bsc4I CCNNNNNNNGG 2 cut(s) 303, 505
Bse1I ACTGG 2 cut(s) 160, 350
Bse3DI GCAATG 1 cut(s) 526
Bse8I GATNNNNATC 2 cut(s) 234, 444
BseBI CCWGG 1 cut(s) 500
BseDI CCNNGG 1 cut(s) 666
BseGI GGATG 3 cut(s) 264, 455, 542
BseJI GATNNNNATC 2 cut(s) 234, 444
BseLI CCNNNNNNNGG 2 cut(s) 303, 505
BseMI GCAATG 1 cut(s) 526
BseMII CTCAG 3 cut(s) 503, 542, 695
BseNI ACTGG 2 cut(s) 160, 350
BseXI GCAGC 1 cut(s) 410
BseYI CCCAGC 1 cut(s) 460
Bsh1236I CGCG 1 cut(s) 656
BsiHKAI GWGCWC 1 cut(s) 654
BslFI GGGAC 1 cut(s) 65
BslI CCNNNNNNNGG 2 cut(s) 303, 505
BsmAI GTCTC 2 cut(s) 624, 699
BsmBI CGTCTC 2 cut(s) 624, 699
BsmFI GGGAC 1 cut(s) 65
Bsp1286I GDGCHC 2 cut(s) 73, 654
Bsp143I GATC 9 cut(s) 4, 46, 141, 172, 235, 271, 382, 423, 431
Bsp1720I GCTNAGC 1 cut(s) 681
Bsp68I TCGCGA 1 cut(s) 656
BspACI CCGC 1 cut(s) 64
BspCNI CTCAG 3 cut(s) 504, 543, 694
BspFNI CGCG 1 cut(s) 656
BspHI TCATGA 1 cut(s) 426
BspLI GGNNCC 2 cut(s) 325, 375
BspPI GGATC 4 cut(s) 12, 54, 136, 167
BsrDI GCAATG 1 cut(s) 526
BsrI ACTGG 2 cut(s) 160, 350
BssECI CCNNGG 1 cut(s) 666
BssMI GATC 9 cut(s) 4, 46, 141, 172, 235, 271, 382, 423, 431
BssT1I CCWWGG 1 cut(s) 666
Bst2UI CCWGG 1 cut(s) 500
Bst4CI ACNGT 4 cut(s) 186, 323, 331, 622
BstAPI GCANNNNNTGC 1 cut(s) 26
BstC8I GCNNGC 1 cut(s) 654
BstDEI CTNAG 3 cut(s) 512, 551, 681
BstF5I GGATG 3 cut(s) 264, 455, 542
BstFNI CGCG 1 cut(s) 656
BstHHI GCGC 2 cut(s) 220, 420
BstKTI GATC 9 cut(s) 7, 49, 144, 175, 238, 274, 385, 426, 434
BstMAI GTCTC 2 cut(s) 624, 699
BstMBI GATC 9 cut(s) 4, 46, 141, 172, 235, 271, 382, 423, 431
BstMWI GCNNNNNNNGC 2 cut(s) 26, 35
BstNI CCWGG 1 cut(s) 500
BstSCI CCNGG 1 cut(s) 498
BstUI CGCG 1 cut(s) 656
BstV1I GCAGC 1 cut(s) 410
BstV2I GAAGAC 1 cut(s) 471
BsuI GTATCC 1 cut(s) 673
BtrI CACGTC 1 cut(s) 634
BtsCI GGATG 3 cut(s) 264, 455, 542
BtsI GCAGTG 1 cut(s) 227
BtsIMutI CAGTG 3 cut(s) 182, 227, 528
BtuMI TCGCGA 1 cut(s) 656
Cac8I GCNNGC 1 cut(s) 654
CciI TCATGA 1 cut(s) 426
CfoI GCGC 2 cut(s) 220, 420
CseI GACGC 1 cut(s) 371
CsiI ACCWGGT 1 cut(s) 498
Csp6I GTAC 3 cut(s) 187, 288, 332
CviAII CATG 6 cut(s) 43, 95, 107, 269, 427, 738
CviJI RGCY 8 cut(s) 38, 56, 71, 241, 255, 464, 680, 685
CviKI_1 RGCY 8 cut(s) 38, 56, 71, 241, 255, 464, 680, 685
CviQI GTAC 3 cut(s) 187, 288, 332
DdeI CTNAG 3 cut(s) 512, 551, 681
DpnI GATC 9 cut(s) 6, 48, 143, 174, 237, 273, 384, 425, 433
DpnII GATC 9 cut(s) 4, 46, 141, 172, 235, 271, 382, 423, 431
EciI GGCGGA 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 666
Eco24I GRGCYC 1 cut(s) 73
EcoRII CCWGG 1 cut(s) 498
EcoT14I CCWWGG 1 cut(s) 666
EcoT38I GRGCYC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 666
Esp3I CGTCTC 2 cut(s) 624, 699
FaeI CATG 6 cut(s) 46, 98, 110, 272, 430, 741
FalI AAGNNNNNCTT 2 cut(s) 463, 495
FaqI GGGAC 1 cut(s) 65
FatI CATG 6 cut(s) 42, 94, 106, 268, 426, 737
FbaI TGATCA 3 cut(s) 271, 382, 423
Fnu4HI GCNGC 1 cut(s) 399
FokI GGATG 3 cut(s) 251, 442, 549
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 1 cut(s) 399
FspBI CTAG 1 cut(s) 411
FspI TGCGCA 1 cut(s) 219
GlaI GCGC 2 cut(s) 219, 419
GluI GCNGC 1 cut(s) 399
GsaI CCCAGC 1 cut(s) 464
HgaI GACGC 1 cut(s) 371
HhaI GCGC 2 cut(s) 220, 420
Hin1II CATG 6 cut(s) 46, 98, 110, 272, 430, 741
Hin6I GCGC 2 cut(s) 218, 418
HinP1I GCGC 2 cut(s) 218, 418
HinfI GANTC 1 cut(s) 78
HphI GGTGA 3 cut(s) 682, 702, 708
Hpy188I TCNGA 7 cut(s) 177, 249, 312, 355, 382, 513, 629
Hpy188III TCNNGA 3 cut(s) 427, 655, 726
Hpy99I CGWCG 2 cut(s) 249, 453
HpyAV CCTTC 5 cut(s) 195, 346, 387, 569, 723
HpyCH4III ACNGT 4 cut(s) 186, 323, 331, 622
HpyCH4IV ACGT 3 cut(s) 244, 368, 633
HpyCH4V TGCA 5 cut(s) 20, 198, 526, 531, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 35
HpyF3I CTNAG 3 cut(s) 512, 551, 681
HpySE526I ACGT 3 cut(s) 244, 368, 633
Hsp92II CATG 6 cut(s) 46, 98, 110, 272, 430, 741
HspAI GCGC 2 cut(s) 218, 418
Ksp22I TGATCA 3 cut(s) 271, 382, 423
Kzo9I GATC 9 cut(s) 4, 46, 141, 172, 235, 271, 382, 423, 431
LmnI GCTCC 1 cut(s) 469
LpnPI CCDG 7 cut(s) 173, 331, 434, 474, 485, 512, 673
Lsp1109I GCAGC 1 cut(s) 410
LweI GCATC 5 cut(s) 29, 185, 410, 568, 593
MabI ACCWGGT 1 cut(s) 498
MaeI CTAG 1 cut(s) 411
MaeII ACGT 3 cut(s) 244, 368, 633
MaeIII GTNAC 5 cut(s) 162, 317, 494, 616, 634
MalI GATC 9 cut(s) 6, 48, 143, 174, 237, 273, 384, 425, 433
MboI GATC 9 cut(s) 4, 46, 141, 172, 235, 271, 382, 423, 431
MfeI CAATTG 1 cut(s) 168
MhlI GDGCHC 2 cut(s) 73, 654
MluCI AATT 1 cut(s) 168
MlyI GAGTC 1 cut(s) 87
MmeI TCCRAC 2 cut(s) 227, 722
MnlI CCTC 2 cut(s) 304, 507
MseI TTAA 1 cut(s) 470
MslI CAYNNNNRTG 1 cut(s) 99
MspR9I CCNGG 1 cut(s) 500
MunI CAATTG 1 cut(s) 168
MvaI CCWGG 1 cut(s) 500
MvnI CGCG 1 cut(s) 656
MwoI GCNNNNNNNGC 2 cut(s) 26, 35
NdeII GATC 9 cut(s) 4, 46, 141, 172, 235, 271, 382, 423, 431
NlaIII CATG 6 cut(s) 46, 98, 110, 272, 430, 741
NlaIV GGNNCC 2 cut(s) 325, 375
NmeAIII GCCGAG 1 cut(s) 92
NmuCI GTSAC 3 cut(s) 317, 616, 634
NruI TCGCGA 1 cut(s) 656
NsbI TGCGCA 1 cut(s) 219
PagI TCATGA 1 cut(s) 426
PflMI CCANNNNNTGG 1 cut(s) 505
PkrI GCNGC 1 cut(s) 400
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
Psp1406I AACGTT 1 cut(s) 368
Psp6I CCWGG 1 cut(s) 498
PspFI CCCAGC 1 cut(s) 460
PspGI CCWGG 1 cut(s) 498
PspN4I GGNNCC 2 cut(s) 325, 375
RruI TCGCGA 1 cut(s) 656
RsaI GTAC 3 cut(s) 188, 289, 333
RsaNI GTAC 3 cut(s) 187, 288, 332
RseI CAYNNNNRTG 1 cut(s) 99
SaqAI TTAA 1 cut(s) 470
SatI GCNGC 1 cut(s) 399
Sau3AI GATC 9 cut(s) 4, 46, 141, 172, 235, 271, 382, 423, 431
ScaI AGTACT 1 cut(s) 289
SchI GAGTC 1 cut(s) 87
ScrFI CCNGG 1 cut(s) 500
SduI GDGCHC 2 cut(s) 73, 654
SexAI ACCWGGT 1 cut(s) 498
SfaNI GCATC 5 cut(s) 29, 185, 410, 568, 593
SmiMI CAYNNNNRTG 1 cut(s) 99
Sse9I AATT 1 cut(s) 168
SsiI CCGC 1 cut(s) 64
SspMI CTAG 1 cut(s) 411
StyD4I CCNGG 1 cut(s) 498
StyI CCWWGG 1 cut(s) 666
TaaI ACNGT 4 cut(s) 186, 323, 331, 622
TaiI ACGT 3 cut(s) 247, 371, 636
TaqI TCGA 4 cut(s) 112, 342, 434, 727
TasI AATT 1 cut(s) 168
TatI WGTACW 1 cut(s) 287
Tru1I TTAA 1 cut(s) 470
Tru9I TTAA 1 cut(s) 470
TscAI CASTG 3 cut(s) 189, 227, 528
TseFI GTSAC 3 cut(s) 317, 616, 634
TseI GCWGC 1 cut(s) 398
Tsp45I GTSAC 3 cut(s) 317, 616, 634
TspDTI ATGAA 5 cut(s) 95, 141, 328, 456, 696
TspRI CASTG 3 cut(s) 189, 227, 528
Van91I CCANNNNNTGG 1 cut(s) 505
XcmI CCANNNNNNNNNTGG 2 cut(s) 40, 726
XspI CTAG 1 cut(s) 411
ZrmI AGTACT 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.