Prupe.6G007500_v2.0.a1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0009518
Physical Location & Seq
Reverse (-)
630847 .. 632817
1971 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G007500.1

Sequence Viewer

Length: 744 bp
ATGAAAGGACTGGATATTTTCTGTGCTTCTCCAGCTTCCACATCCATTTGCTCTAGCATGGACCAACATGCCATGGTCCGCCTAGGCCCCGCATCCATTGATCATCCTCATCATCATCCTCATCATCGTCTTCATGATCGACCCAAAAACCAGCCTCACAATCAAACTCACCATCACCATGTCCCTTGCTCATCTCAGCTGCCAATTGATCCCAAACCATTTTATGAGAAATGTAGGAAGAGTTTTTCTAGTGCACAAACTCAGCTACGTAGAAAAAGCTCTGCTGACATTCATGACTTAACAAGGACTAGGAGCCTCAATGGTTCTTCTTCTTCTCGGTATCTTTTAAGTGACTCGCCCTTCGTAGGCTGGTTATCGGAGGCCGATCGGAATGTTTCTGCTAATACAATATTGCTCCCTCGCCAAGTGCCTTCTAAGCCTAGACGGTTGATGATCTCAAGTGATCACTCTCCTGCTTTAAGGTCTTCTTCCACTCGTTCTCGTCACCAGGTTGTTGTTTTGAGGGTGTCACTTCATTGCAAGGGCTGTGAAGGAAAAGTGAGAAAGCATCTTTCTAAAATGGAAGGAGTGACATCATTTAGTATAGATTTTCCAACCAAAAAGGTCACAGTCATTGGAGATGTTACCCCATTAGGCGTGCTCTCAAGTGTCTCCAAGGTGAAGAAGGCTCAGCTCTGGCCTTCTCCAACATCATCATCACCATCCTCTCGATGGTCAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

248

Amino Acids

27.42

Weight (kDa)

10.29

Isoelectric Point (pI)

59.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 79, 90
AclWI GGATC 1 cut(s) 203
AfiI CCNNNNNNNGG 2 cut(s) 365, 732
AjnI CCWGG 1 cut(s) 507
AluBI AGCT 5 cut(s) 35, 199, 265, 279, 694
AluI AGCT 5 cut(s) 35, 199, 265, 279, 694
Alw21I GWGCWC 2 cut(s) 256, 663
Alw26I GTCTC 1 cut(s) 676
Alw44I GTGCAC 1 cut(s) 252
AlwI GGATC 1 cut(s) 203
AoxI GGCC 3 cut(s) 85, 381, 698
ApaLI GTGCAC 1 cut(s) 252
ApeKI GCWGC 1 cut(s) 199
ArsI GACNNNNNNTTYG 2 cut(s) 344, 376
AspA2I CCTAGG 1 cut(s) 82
AspS9I GGNCC 3 cut(s) 61, 76, 86
AsuHPI GGTGA 5 cut(s) 161, 167, 497, 691, 711
AvaII GGWCC 2 cut(s) 61, 76
AvrII CCTAGG 1 cut(s) 82
BaeGI GKGCMC 1 cut(s) 256
BbsI GAAGAC 2 cut(s) 122, 477
Bbv12I GWGCWC 2 cut(s) 256, 663
BbvI GCAGC 1 cut(s) 186
BccI CCATC 3 cut(s) 180, 726, 730
BciT130I CCWGG 1 cut(s) 509
BclI TGATCA 2 cut(s) 100, 463
BcoDI GTCTC 1 cut(s) 676
BfaI CTAG 5 cut(s) 54, 83, 249, 309, 441
BisI GCNGC 1 cut(s) 200
BlnI CCTAGG 1 cut(s) 82
BlpI GCTNAGC 1 cut(s) 690
BlsI GCNGC 1 cut(s) 201
Bme1390I CCNGG 1 cut(s) 509
Bme18I GGWCC 2 cut(s) 61, 76
BmgT120I GGNCC 3 cut(s) 61, 76, 86
BmiI GGNNCC 2 cut(s) 88, 314
BmrFI CCNGG 1 cut(s) 509
BmsI GCATC 2 cut(s) 101, 577
BpiI GAAGAC 2 cut(s) 122, 477
BpmI CTGGAG 1 cut(s) 15
Bpu1102I GCTNAGC 1 cut(s) 690
BpuEI CTTGAG 2 cut(s) 442, 649
BsaAI YACGTR 1 cut(s) 269
BsaJI CCNNGG 3 cut(s) 72, 82, 675
Bsc4I CCNNNNNNNGG 2 cut(s) 365, 732
Bse1I ACTGG 1 cut(s) 15
Bse3DI GCAATG 1 cut(s) 535
BseBI CCWGG 1 cut(s) 509
BseDI CCNNGG 3 cut(s) 72, 82, 675
BseGI GGATG 5 cut(s) 41, 92, 103, 115, 722
BseLI CCNNNNNNNGG 2 cut(s) 365, 732
BseMI GCAATG 1 cut(s) 535
BseMII CTCAG 3 cut(s) 209, 275, 704
BseNI ACTGG 1 cut(s) 15
BseSI GKGCMC 1 cut(s) 256
BseXI GCAGC 1 cut(s) 186
Bsh1285I CGRYCG 1 cut(s) 388
BshFI GGCC 3 cut(s) 87, 383, 700
BsiEI CGRYCG 1 cut(s) 388
BsiHKAI GWGCWC 2 cut(s) 256, 663
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 2 cut(s) 365, 732
BsmAI GTCTC 1 cut(s) 676
BsmFI GGGAC 1 cut(s) 167
BsnI GGCC 3 cut(s) 87, 383, 700
Bsp1286I GDGCHC 2 cut(s) 256, 663
Bsp143I GATC 6 cut(s) 100, 136, 208, 385, 453, 463
Bsp1720I GCTNAGC 1 cut(s) 690
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 2 cut(s) 79, 90
BspANI GGCC 3 cut(s) 87, 383, 700
BspCNI CTCAG 3 cut(s) 208, 274, 703
BspHI TCATGA 2 cut(s) 133, 292
BspLI GGNNCC 2 cut(s) 88, 314
BspPI GGATC 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 535
BsrI ACTGG 1 cut(s) 15
BssECI CCNNGG 3 cut(s) 72, 82, 675
BssMI GATC 6 cut(s) 100, 136, 208, 385, 453, 463
BssT1I CCWWGG 3 cut(s) 72, 82, 675
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 2 cut(s) 447, 631
Bst6I CTCTTC 1 cut(s) 233
BstBAI YACGTR 1 cut(s) 269
BstC8I GCNNGC 1 cut(s) 659
BstDEI CTNAG 4 cut(s) 195, 261, 435, 690
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 5 cut(s) 41, 92, 103, 115, 722
BstKTI GATC 6 cut(s) 103, 139, 211, 388, 456, 466
BstMAI GTCTC 1 cut(s) 676
BstMBI GATC 6 cut(s) 100, 136, 208, 385, 453, 463
BstMCI CGRYCG 1 cut(s) 388
BstMWI GCNNNNNNNGC 2 cut(s) 32, 436
BstNI CCWGG 1 cut(s) 509
BstNSI RCATGY 1 cut(s) 71
BstSCI CCNGG 1 cut(s) 507
BstSLI GKGCMC 1 cut(s) 256
BstSNI TACGTA 1 cut(s) 269
BstV1I GCAGC 1 cut(s) 186
BstV2I GAAGAC 2 cut(s) 122, 477
BsuRI GGCC 3 cut(s) 87, 383, 700
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 5 cut(s) 41, 92, 103, 115, 722
Cac8I GCNNGC 1 cut(s) 659
CciI TCATGA 2 cut(s) 133, 292
Cfr13I GGNCC 3 cut(s) 61, 76, 86
CsiI ACCWGGT 1 cut(s) 507
CviAII CATG 7 cut(s) 58, 68, 73, 134, 179, 293, 741
DdeI CTNAG 4 cut(s) 195, 261, 435, 690
DpnI GATC 6 cut(s) 102, 138, 210, 387, 455, 465
DpnII GATC 6 cut(s) 100, 136, 208, 385, 453, 463
Eam1104I CTCTTC 1 cut(s) 233
EarI CTCTTC 1 cut(s) 233
EciI GGCGGA 1 cut(s) 68
Eco105I TACGTA 1 cut(s) 269
Eco130I CCWWGG 3 cut(s) 72, 82, 675
Eco47I GGWCC 2 cut(s) 61, 76
EcoO109I RGGNCCY 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 507
EcoT14I CCWWGG 3 cut(s) 72, 82, 675
ErhI CCWWGG 3 cut(s) 72, 82, 675
FaeI CATG 7 cut(s) 61, 71, 76, 137, 182, 296, 744
FaiI YATR 9 cut(s) 59, 69, 74, 135, 180, 225, 294, 605, 742
FalI AAGNNNNNCTT 2 cut(s) 472, 504
FaqI GGGAC 1 cut(s) 167
FatI CATG 7 cut(s) 57, 67, 72, 133, 178, 292, 740
FauI CCCGC 1 cut(s) 97
FbaI TGATCA 2 cut(s) 100, 463
Fnu4HI GCNGC 1 cut(s) 200
FokI GGATG 5 cut(s) 28, 79, 90, 102, 709
Fsp4HI GCNGC 1 cut(s) 200
FspBI CTAG 5 cut(s) 54, 83, 249, 309, 441
GluI GCNGC 1 cut(s) 200
GsuI CTGGAG 1 cut(s) 15
HaeIII GGCC 3 cut(s) 87, 383, 700
Hin1II CATG 7 cut(s) 61, 71, 76, 137, 182, 296, 744
HinfI GANTC 1 cut(s) 353
HphI GGTGA 5 cut(s) 161, 167, 497, 691, 711
Hpy166II GTNNAC 1 cut(s) 254
Hpy188I TCNGA 2 cut(s) 379, 390
Hpy188III TCNNGA 3 cut(s) 134, 293, 729
Hpy8I GTNNAC 1 cut(s) 254
HpyAV CCTTC 6 cut(s) 370, 441, 545, 578, 679, 711
HpyCH4III ACNGT 2 cut(s) 447, 631
HpyCH4IV ACGT 1 cut(s) 268
HpyCH4V TGCA 2 cut(s) 254, 540
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 436
HpyF3I CTNAG 4 cut(s) 195, 261, 435, 690
HpySE526I ACGT 1 cut(s) 268
Hsp92II CATG 7 cut(s) 61, 71, 76, 137, 182, 296, 744
Ksp22I TGATCA 2 cut(s) 100, 463
Kzo9I GATC 6 cut(s) 100, 136, 208, 385, 453, 463
LmnI GCTCC 2 cut(s) 312, 420
LpnPI CCDG 7 cut(s) 45, 164, 355, 486, 494, 521, 682
Lsp1109I GCAGC 1 cut(s) 186
LweI GCATC 2 cut(s) 101, 577
MabI ACCWGGT 1 cut(s) 507
MaeI CTAG 5 cut(s) 54, 83, 249, 309, 441
MaeII ACGT 1 cut(s) 268
MaeIII GTNAC 6 cut(s) 350, 503, 528, 589, 625, 643
MalI GATC 6 cut(s) 102, 138, 210, 387, 455, 465
MboI GATC 6 cut(s) 100, 136, 208, 385, 453, 463
MboII GAAGA 8 cut(s) 122, 250, 318, 321, 324, 477, 480, 694
MfeI CAATTG 1 cut(s) 204
MhlI GDGCHC 2 cut(s) 256, 663
MluCI AATT 1 cut(s) 204
MlyI GAGTC 1 cut(s) 347
MmeI TCCRAC 2 cut(s) 638, 731
MnlI CCTC 8 cut(s) 117, 129, 165, 326, 373, 429, 516, 736
MseI TTAA 3 cut(s) 299, 347, 479
MslI CAYNNNNRTG 1 cut(s) 177
MspA1I CMGCKG 1 cut(s) 199
MspR9I CCNGG 1 cut(s) 509
MunI CAATTG 1 cut(s) 204
MvaI CCWGG 1 cut(s) 509
MwoI GCNNNNNNNGC 2 cut(s) 32, 436
NcoI CCATGG 1 cut(s) 72
NdeII GATC 6 cut(s) 100, 136, 208, 385, 453, 463
NlaIII CATG 7 cut(s) 61, 71, 76, 137, 182, 296, 744
NlaIV GGNNCC 2 cut(s) 88, 314
NmuCI GTSAC 5 cut(s) 350, 503, 528, 589, 625
NspI RCATGY 1 cut(s) 71
PagI TCATGA 2 cut(s) 133, 292
PkrI GCNGC 1 cut(s) 201
Ple19I CGATCG 1 cut(s) 388
PleI GAGTC 1 cut(s) 347
PpsI GAGTC 1 cut(s) 347
Ppu21I YACGTR 1 cut(s) 269
Psp6I CCWGG 1 cut(s) 507
PspGI CCWGG 1 cut(s) 507
PspN4I GGNNCC 2 cut(s) 88, 314
PspPI GGNCC 3 cut(s) 61, 76, 86
PvuI CGATCG 1 cut(s) 388
PvuII CAGCTG 1 cut(s) 199
RseI CAYNNNNRTG 1 cut(s) 177
SaqAI TTAA 3 cut(s) 299, 347, 479
SatI GCNGC 1 cut(s) 200
Sau3AI GATC 6 cut(s) 100, 136, 208, 385, 453, 463
Sau96I GGNCC 3 cut(s) 61, 76, 86
SchI GAGTC 1 cut(s) 347
ScrFI CCNGG 1 cut(s) 509
SduI GDGCHC 2 cut(s) 256, 663
SexAI ACCWGGT 1 cut(s) 507
SfaNI GCATC 2 cut(s) 101, 577
SinI GGWCC 2 cut(s) 61, 76
SmiMI CAYNNNNRTG 1 cut(s) 177
SmlI CTYRAG 2 cut(s) 457, 664
SmoI CTYRAG 2 cut(s) 457, 664
SnaBI TACGTA 1 cut(s) 269
Sse9I AATT 1 cut(s) 204
SsiI CCGC 2 cut(s) 79, 90
SspI AATATT 1 cut(s) 411
SspMI CTAG 5 cut(s) 54, 83, 249, 309, 441
StyD4I CCNGG 1 cut(s) 507
StyI CCWWGG 3 cut(s) 72, 82, 675
TaaI ACNGT 2 cut(s) 447, 631
TaiI ACGT 1 cut(s) 271
TaqI TCGA 2 cut(s) 139, 730
TasI AATT 1 cut(s) 204
Tru1I TTAA 3 cut(s) 299, 347, 479
Tru9I TTAA 3 cut(s) 299, 347, 479
TseFI GTSAC 5 cut(s) 350, 503, 528, 589, 625
TseI GCWGC 1 cut(s) 199
Tsp45I GTSAC 5 cut(s) 350, 503, 528, 589, 625
TspDTI ATGAA 4 cut(s) 17, 122, 281, 524
VneI GTGCAC 1 cut(s) 252
VpaK11BI GGWCC 2 cut(s) 61, 76
XceI RCATGY 1 cut(s) 71
XcmI CCANNNNNNNNNTGG 1 cut(s) 729
XmaJI CCTAGG 1 cut(s) 82
XspI CTAG 5 cut(s) 54, 83, 249, 309, 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.