pycom11g09760

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0009518
Physical Location & Seq
Reverse (-)
8076351 .. 8077753
1403 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g09760.1

Sequence Viewer

Length: 846 bp
ATGAAAGGAATGGATATTTTCTGTGCATCTCCAGCTTCCACAGCCATTTGCTCTAGCCTGGATGCCGTGGTCCGCCGAGGCCCCAGACCCCTTGATCATGGTCATTATCATCGTCTTCATGATCACGATCAACGGAAAAACCAACCTCATCATCAAACTCACCATGCCCCTTGCTCATCTCAGTTACCTATTGATCCCAAACCATTTTACGAAATATGTAGAAAGAGTTTTTCTAGTCCACAAAGGCAGCTACGTACAAAAAGCTCTGCTGAGATACATGACTTAACAAGGACTAAGAGTGCCAATGGCTCTTCTTCTTCTCCTTCTCGGTTCCTCTTAAGCGACTCGCCCTTCATTGACAGGATATCAGAGCCCGATTGTAAGAATGTCTCTGCAGCTGCATTTGTTCCTCCTCAAGTACCCTCTAAGCCTAGAGACTCGATGAGCTCAAATATTCCTCCTCAAGTACCCTCTAAAGTACCCTCTAAGCCTAGAGACCCGATGAGCTCAAATGTTCCTCCTCAAGTACCCTCTAAGCCTAGAGGCTCGATGAGCTCAAATGGTCATTGTAGTACTGTTTTGAAGTCTTCTTCTACTAGTTCTCGTCACCAGGTTGTTGTTTTGAGGGTGTCACTGCATTGCAAGGGTTGTGAAGGAAAAGTGAGAAAACACCTCTCTAAAATGGAAGGAGTGACTTCATTTAGCATAGATTTCCAAACAAAGAGAGTCACTGTGGTGGGGGATGTCACCACATTAGGTGTGCTTTCAAGTGTATCAAAGGTGAGGAAATCCCAGCTCTGGCCTTCTCCAACATCATCATCACCATCCTCTCGGTTGCCAGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

282

Amino Acids

30.64

Weight (kDa)

10.07

Isoelectric Point (pI)

65.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 208 - 251 2.1e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 73
AclWI GGATC 1 cut(s) 188
AfaI GTAC 6 cut(s) 256, 420, 468, 480, 528, 574
AfiI CCNNNNNNNGG 1 cut(s) 798
AflII CTTAAG 1 cut(s) 337
AgsI TTSAA 2 cut(s) 583, 768
AhlI ACTAGT 1 cut(s) 596
AjnI CCWGG 2 cut(s) 57, 609
AleI CACNNNNGTG 1 cut(s) 734
AluBI AGCT 8 cut(s) 35, 250, 264, 398, 447, 507, 555, 796
AluI AGCT 8 cut(s) 35, 250, 264, 398, 447, 507, 555, 796
Alw21I GWGCWC 3 cut(s) 449, 509, 557
Alw26I GTCTC 3 cut(s) 394, 429, 489
AlwI GGATC 1 cut(s) 188
AoxI GGCC 2 cut(s) 79, 800
ApeKI GCWGC 3 cut(s) 247, 395, 398
AspS9I GGNCC 2 cut(s) 70, 80
AsuHPI GGTGA 5 cut(s) 152, 599, 739, 793, 813
AvaII GGWCC 1 cut(s) 70
BanII GRGCYC 4 cut(s) 375, 449, 509, 557
BbsI GAAGAC 2 cut(s) 107, 579
Bbv12I GWGCWC 3 cut(s) 449, 509, 557
BbvI GCAGC 3 cut(s) 259, 385, 407
BccI CCATC 1 cut(s) 832
BceAI ACGGC 1 cut(s) 50
BciT130I CCWGG 2 cut(s) 59, 611
BclI TGATCA 2 cut(s) 94, 121
BcoDI GTCTC 3 cut(s) 394, 429, 489
BcuI ACTAGT 1 cut(s) 596
BfaI CTAG 6 cut(s) 54, 234, 432, 492, 540, 597
BfmI CTRYAG 1 cut(s) 393
BfrI CTTAAG 1 cut(s) 337
BisI GCNGC 3 cut(s) 248, 396, 399
BlsI GCNGC 3 cut(s) 249, 397, 400
BmcAI AGTACT 1 cut(s) 574
Bme1390I CCNGG 2 cut(s) 59, 611
Bme18I GGWCC 1 cut(s) 70
BmgT120I GGNCC 2 cut(s) 70, 80
BmiI GGNNCC 2 cut(s) 82, 332
BmrFI CCNGG 2 cut(s) 59, 611
BmsI GCATC 2 cut(s) 35, 52
BpiI GAAGAC 2 cut(s) 107, 579
BpmI CTGGAG 1 cut(s) 15
BpuEI CTTGAG 3 cut(s) 399, 447, 507
BsaAI YACGTR 1 cut(s) 254
BsaBI GATNNNNATC 1 cut(s) 126
BsaI GGTCTC 1 cut(s) 489
BsaJI CCNNGG 2 cut(s) 66, 76
Bsc4I CCNNNNNNNGG 1 cut(s) 798
Bse3DI GCAATG 1 cut(s) 637
Bse8I GATNNNNATC 1 cut(s) 126
BseBI CCWGG 2 cut(s) 59, 611
BseDI CCNNGG 2 cut(s) 66, 76
BseGI GGATG 3 cut(s) 67, 748, 824
BseJI GATNNNNATC 1 cut(s) 126
BseLI CCNNNNNNNGG 1 cut(s) 798
BseMI GCAATG 1 cut(s) 637
BseMII CTCAG 2 cut(s) 194, 261
BseRI GAGGAG 3 cut(s) 402, 450, 510
BseXI GCAGC 3 cut(s) 259, 385, 407
BseYI CCCAGC 1 cut(s) 792
BshFI GGCC 2 cut(s) 81, 802
BsiHKAI GWGCWC 3 cut(s) 449, 509, 557
BslI CCNNNNNNNGG 1 cut(s) 798
BsmAI GTCTC 3 cut(s) 394, 429, 489
BsnI GGCC 2 cut(s) 81, 802
Bso31I GGTCTC 1 cut(s) 489
Bsp1286I GDGCHC 4 cut(s) 375, 449, 509, 557
Bsp143I GATC 4 cut(s) 94, 121, 127, 193
BspACI CCGC 1 cut(s) 73
BspANI GGCC 2 cut(s) 81, 802
BspCNI CTCAG 2 cut(s) 193, 262
BspHI TCATGA 1 cut(s) 118
BspLI GGNNCC 2 cut(s) 82, 332
BspMAI CTGCAG 1 cut(s) 397
BspPI GGATC 1 cut(s) 188
BspQI GCTCTTC 1 cut(s) 316
BspTI CTTAAG 1 cut(s) 337
BspTNI GGTCTC 1 cut(s) 489
BsrDI GCAATG 1 cut(s) 637
BssECI CCNNGG 2 cut(s) 66, 76
BssMI GATC 4 cut(s) 94, 121, 127, 193
Bst2UI CCWGG 2 cut(s) 59, 611
Bst4CI ACNGT 2 cut(s) 577, 733
Bst6I CTCTTC 1 cut(s) 316
BstAFI CTTAAG 1 cut(s) 337
BstBAI YACGTR 1 cut(s) 254
BstC8I GCNNGC 1 cut(s) 840
BstDEI CTNAG 6 cut(s) 180, 270, 294, 426, 486, 534
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 3 cut(s) 67, 748, 824
BstKTI GATC 4 cut(s) 97, 124, 130, 196
BstMAI GTCTC 3 cut(s) 394, 429, 489
BstMBI GATC 4 cut(s) 94, 121, 127, 193
BstMWI GCNNNNNNNGC 3 cut(s) 32, 41, 552
BstNI CCWGG 2 cut(s) 59, 611
BstSCI CCNGG 2 cut(s) 57, 609
BstSFI CTRYAG 1 cut(s) 393
BstSNI TACGTA 1 cut(s) 254
BstV1I GCAGC 3 cut(s) 259, 385, 407
BstV2I GAAGAC 2 cut(s) 107, 579
BsuRI GGCC 2 cut(s) 81, 802
BtgI CCRYGG 1 cut(s) 66
BtsCI GGATG 3 cut(s) 67, 748, 824
BtsI GCAGTG 1 cut(s) 632
BtsIMutI CAGTG 2 cut(s) 632, 729
Cac8I GCNNGC 1 cut(s) 840
CciI TCATGA 1 cut(s) 118
Cfr13I GGNCC 2 cut(s) 70, 80
CsiI ACCWGGT 1 cut(s) 609
Csp6I GTAC 6 cut(s) 255, 419, 467, 479, 527, 573
CviAII CATG 4 cut(s) 98, 119, 164, 278
CviQI GTAC 6 cut(s) 255, 419, 467, 479, 527, 573
DdeI CTNAG 6 cut(s) 180, 270, 294, 426, 486, 534
DpnI GATC 4 cut(s) 96, 123, 129, 195
DpnII GATC 4 cut(s) 94, 121, 127, 193
Eam1104I CTCTTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 316
EciI GGCGGA 1 cut(s) 62
Ecl136II GAGCTC 3 cut(s) 447, 507, 555
Eco105I TACGTA 1 cut(s) 254
Eco24I GRGCYC 4 cut(s) 375, 449, 509, 557
Eco31I GGTCTC 1 cut(s) 489
Eco32I GATATC 1 cut(s) 366
Eco47I GGWCC 1 cut(s) 70
Eco53kI GAGCTC 3 cut(s) 447, 507, 555
EcoICRI GAGCTC 3 cut(s) 447, 507, 555
EcoO109I RGGNCCY 1 cut(s) 80
EcoRII CCWGG 2 cut(s) 57, 609
EcoRV GATATC 1 cut(s) 366
EcoT38I GRGCYC 4 cut(s) 375, 449, 509, 557
FaeI CATG 4 cut(s) 101, 122, 167, 281
FaiI YATR 6 cut(s) 99, 120, 165, 217, 279, 707
FatI CATG 4 cut(s) 97, 118, 163, 277
FbaI TGATCA 2 cut(s) 94, 121
Fnu4HI GCNGC 3 cut(s) 248, 396, 399
FokI GGATG 3 cut(s) 74, 755, 811
FriOI GRGCYC 4 cut(s) 375, 449, 509, 557
Fsp4HI GCNGC 3 cut(s) 248, 396, 399
FspBI CTAG 6 cut(s) 54, 234, 432, 492, 540, 597
GluI GCNGC 3 cut(s) 248, 396, 399
GsaI CCCAGC 1 cut(s) 796
GsuI CTGGAG 1 cut(s) 15
HaeIII GGCC 2 cut(s) 81, 802
Hin1II CATG 4 cut(s) 101, 122, 167, 281
HinfI GANTC 3 cut(s) 344, 437, 726
HphI GGTGA 5 cut(s) 152, 599, 739, 793, 813
Hpy166II GTNNAC 1 cut(s) 239
Hpy188I TCNGA 1 cut(s) 370
Hpy188III TCNNGA 2 cut(s) 119, 125
Hpy8I GTNNAC 1 cut(s) 239
HpyAV CCTTC 5 cut(s) 333, 361, 647, 680, 813
HpyCH4III ACNGT 2 cut(s) 577, 733
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 5 cut(s) 26, 395, 401, 637, 642
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 41, 552
HpyF3I CTNAG 6 cut(s) 180, 270, 294, 426, 486, 534
HpySE526I ACGT 1 cut(s) 253
Hsp92II CATG 4 cut(s) 101, 122, 167, 281
Ksp22I TGATCA 2 cut(s) 94, 121
Kzo9I GATC 4 cut(s) 94, 121, 127, 193
LguI GCTCTTC 1 cut(s) 316
LpnPI CCDG 9 cut(s) 44, 45, 71, 97, 346, 596, 623, 784, 806
Lsp1109I GCAGC 3 cut(s) 259, 385, 407
LweI GCATC 2 cut(s) 35, 52
MabI ACCWGGT 1 cut(s) 609
MaeI CTAG 6 cut(s) 54, 234, 432, 492, 540, 597
MaeII ACGT 1 cut(s) 253
MaeIII GTNAC 6 cut(s) 183, 605, 630, 691, 727, 745
MalI GATC 4 cut(s) 96, 123, 129, 195
MboI GATC 4 cut(s) 94, 121, 127, 193
MboII GAAGA 6 cut(s) 107, 303, 306, 309, 579, 582
MhlI GDGCHC 4 cut(s) 375, 449, 509, 557
MlyI GAGTC 3 cut(s) 338, 431, 735
MmeI TCCRAC 1 cut(s) 833
MseI TTAA 2 cut(s) 284, 338
MslI CAYNNNNRTG 1 cut(s) 734
MspA1I CMGCKG 1 cut(s) 398
MspCI CTTAAG 1 cut(s) 337
MspR9I CCNGG 2 cut(s) 59, 611
MvaI CCWGG 2 cut(s) 59, 611
MwoI GCNNNNNNNGC 3 cut(s) 32, 41, 552
NdeII GATC 4 cut(s) 94, 121, 127, 193
NlaIII CATG 4 cut(s) 101, 122, 167, 281
NlaIV GGNNCC 2 cut(s) 82, 332
NmeAIII GCCGAG 1 cut(s) 101
NmuCI GTSAC 5 cut(s) 605, 630, 691, 727, 745
OliI CACNNNNGTG 1 cut(s) 734
PagI TCATGA 1 cut(s) 118
PciSI GCTCTTC 1 cut(s) 316
PkrI GCNGC 3 cut(s) 249, 397, 400
PleI GAGTC 3 cut(s) 338, 431, 734
PpsI GAGTC 3 cut(s) 338, 431, 734
Ppu21I YACGTR 1 cut(s) 254
Psp124BI GAGCTC 3 cut(s) 449, 509, 557
Psp6I CCWGG 2 cut(s) 57, 609
PspFI CCCAGC 1 cut(s) 792
PspGI CCWGG 2 cut(s) 57, 609
PspN4I GGNNCC 2 cut(s) 82, 332
PspPI GGNCC 2 cut(s) 70, 80
PstI CTGCAG 1 cut(s) 397
PvuII CAGCTG 1 cut(s) 398
RsaI GTAC 6 cut(s) 256, 420, 468, 480, 528, 574
RsaNI GTAC 6 cut(s) 255, 419, 467, 479, 527, 573
RseI CAYNNNNRTG 1 cut(s) 734
SacI GAGCTC 3 cut(s) 449, 509, 557
SapI GCTCTTC 1 cut(s) 316
SaqAI TTAA 2 cut(s) 284, 338
SatI GCNGC 3 cut(s) 248, 396, 399
Sau3AI GATC 4 cut(s) 94, 121, 127, 193
Sau96I GGNCC 2 cut(s) 70, 80
ScaI AGTACT 1 cut(s) 574
SchI GAGTC 3 cut(s) 338, 431, 735
ScrFI CCNGG 2 cut(s) 59, 611
SduI GDGCHC 4 cut(s) 375, 449, 509, 557
SexAI ACCWGGT 1 cut(s) 609
SfaNI GCATC 2 cut(s) 35, 52
SfcI CTRYAG 1 cut(s) 393
SinI GGWCC 1 cut(s) 70
SmiMI CAYNNNNRTG 1 cut(s) 734
SmlI CTYRAG 4 cut(s) 337, 414, 462, 522
SmoI CTYRAG 4 cut(s) 337, 414, 462, 522
SnaBI TACGTA 1 cut(s) 254
SpeI ACTAGT 1 cut(s) 596
SsiI CCGC 1 cut(s) 73
SspI AATATT 1 cut(s) 454
SspMI CTAG 6 cut(s) 54, 234, 432, 492, 540, 597
SstI GAGCTC 3 cut(s) 449, 509, 557
StyD4I CCNGG 2 cut(s) 57, 609
TaaI ACNGT 2 cut(s) 577, 733
TaiI ACGT 1 cut(s) 256
TaqI TCGA 2 cut(s) 440, 548
TatI WGTACW 1 cut(s) 572
Tru1I TTAA 2 cut(s) 284, 338
Tru9I TTAA 2 cut(s) 284, 338
TscAI CASTG 2 cut(s) 639, 736
TseFI GTSAC 5 cut(s) 605, 630, 691, 727, 745
TseI GCWGC 3 cut(s) 247, 395, 398
Tsp45I GTSAC 5 cut(s) 605, 630, 691, 727, 745
TspDTI ATGAA 4 cut(s) 17, 107, 343, 687
TspGWI ACGGA 1 cut(s) 148
TspRI CASTG 2 cut(s) 639, 736
Vha464I CTTAAG 1 cut(s) 337
VpaK11BI GGWCC 1 cut(s) 70
XspI CTAG 6 cut(s) 54, 234, 432, 492, 540, 597
ZrmI AGTACT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.