MD03G1102100.v1.1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009518
Physical Location & Seq
Reverse (-)
8742445 .. 8744091
1647 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1102100.v1.1.491

Sequence Viewer

Length: 726 bp
ATGAAAGGAATGGATATTTTCTGTGCATCTCCAGCTTCCACAGCCATTTGCTCTAGCCTGGACCAACGTGCTATGGTCCGCCAAGGCCCCGGGCCCCTTGACCATCGTCATCACTATCATCTTCGTGATCGCGATCAACTGAAAAACCAACCTCATCGTCAAACTCACCATGCCCCTTGCTCATCTCAGTTACCTATCGATCCCAAACCGTTTTACGAGAAATGTAGAAAGAGTTTTTCTAGTGCACAAAGTCAGCCACGCAGAAAGAGATCTGCTGACATACATGACTTAACAAGGACTAAGGGTACCAATGGCTCTTCTTCTTCTCGGTTTCTCTTAAGTGACTCGCCCTTCATCGACTGGATACAGAAGCCTGATCATAAGAATCTCTCGGCTGCGTTTGTTCCTCCTGAAGTACCCTCTAAGCCTAGAGGCTTGATGAGCTCAAATGGTCATTCTAATACTGCCTTGAAGTCTTCTTCTACTCGTTTTCGTCACCAGGTTGTTGTTTTAAGGGTGTCAATGCACTGCAAGGGTTGTGAAGGAAAAGTGAGAAAGCACCTCTCTAAAATGGAAGGAGTGACTTCATTTAGCATAGATTTCCCGACAAAGAAAGTCACGGTGATGGGGGACGTGACCCCATCGGCTGTGCTCTCAAGCGTATCCAAGGTGAAGAAAGCCCAGCTCTGGCCTTCGCCAACATCATCATCACCCTCATCAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

242

Amino Acids

26.65

Weight (kDa)

10.17

Isoelectric Point (pI)

56.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 171 - 224 4.4e-15 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 305
AccB1I GGYRCC 1 cut(s) 305
AccII CGCG 1 cut(s) 132
AciI CCGC 1 cut(s) 79
AclWI GGATC 1 cut(s) 194
AcuI CTGAAG 1 cut(s) 432
AfaI GTAC 2 cut(s) 307, 417
AfiI CCNNNNNNNGG 1 cut(s) 687
AflII CTTAAG 1 cut(s) 337
AgsI TTSAA 1 cut(s) 472
AjiI CACGTC 1 cut(s) 634
AjnI CCWGG 2 cut(s) 57, 498
AluBI AGCT 3 cut(s) 35, 444, 685
AluI AGCT 3 cut(s) 35, 444, 685
Alw21I GWGCWC 3 cut(s) 247, 446, 654
Alw44I GTGCAC 1 cut(s) 243
AlwI GGATC 1 cut(s) 194
Ama87I CYCGRG 1 cut(s) 89
AoxI GGCC 3 cut(s) 85, 92, 689
ApaI GGGCCC 1 cut(s) 96
ApaLI GTGCAC 1 cut(s) 243
ApeKI GCWGC 1 cut(s) 395
Asp718I GGTACC 1 cut(s) 305
AspS9I GGNCC 5 cut(s) 61, 76, 86, 92, 93
AsuC2I CCSGG 2 cut(s) 90, 91
AsuHPI GGTGA 5 cut(s) 158, 488, 634, 682, 702
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 2 cut(s) 61, 76
BaeGI GKGCMC 2 cut(s) 96, 247
BanI GGYRCC 1 cut(s) 305
BanII GRGCYC 2 cut(s) 96, 446
BbsI GAAGAC 1 cut(s) 468
Bbv12I GWGCWC 3 cut(s) 247, 446, 654
BbvI GCAGC 1 cut(s) 382
BccI CCATC 3 cut(s) 111, 619, 649
BciT130I CCWGG 2 cut(s) 59, 500
BciVI GTATCC 2 cut(s) 357, 673
BclI TGATCA 1 cut(s) 376
BcnI CCSGG 2 cut(s) 90, 91
BfaI CTAG 3 cut(s) 54, 240, 429
BfrI CTTAAG 1 cut(s) 337
BfuI GTATCC 2 cut(s) 357, 673
BglII AGATCT 1 cut(s) 269
BisI GCNGC 1 cut(s) 396
BlsI GCNGC 1 cut(s) 397
Bme1390I CCNGG 4 cut(s) 59, 90, 91, 500
Bme18I GGWCC 2 cut(s) 61, 76
BmeT110I CYCGRG 1 cut(s) 89
BmgBI CACGTC 1 cut(s) 634
BmgT120I GGNCC 5 cut(s) 61, 76, 86, 92, 93
BmiI GGNNCC 4 cut(s) 88, 94, 95, 307
BmrFI CCNGG 4 cut(s) 59, 90, 91, 500
BmsI GCATC 1 cut(s) 35
BoxI GACNNNNGTC 1 cut(s) 105
BpiI GAAGAC 1 cut(s) 468
BpmI CTGGAG 1 cut(s) 15
BpuEI CTTGAG 1 cut(s) 640
BpuMI CCSGG 2 cut(s) 90, 91
Bsa29I ATCGAT 1 cut(s) 198
BsaBI GATNNNNATC 1 cut(s) 132
BsaJI CCNNGG 4 cut(s) 82, 88, 89, 666
Bsc4I CCNNNNNNNGG 1 cut(s) 687
Bse1I ACTGG 1 cut(s) 365
Bse8I GATNNNNATC 1 cut(s) 132
BseBI CCWGG 2 cut(s) 59, 500
BseCI ATCGAT 1 cut(s) 198
BseDI CCNNGG 4 cut(s) 82, 88, 89, 666
BseJI GATNNNNATC 1 cut(s) 132
BseLI CCNNNNNNNGG 1 cut(s) 687
BseMII CTCAG 1 cut(s) 200
BseNI ACTGG 1 cut(s) 365
BseSI GKGCMC 2 cut(s) 96, 247
BseXI GCAGC 1 cut(s) 382
BseYI CCCAGC 1 cut(s) 681
Bsh1236I CGCG 1 cut(s) 132
BshFI GGCC 3 cut(s) 87, 94, 691
BshNI GGYRCC 1 cut(s) 305
BshVI ATCGAT 1 cut(s) 198
BsiHKAI GWGCWC 3 cut(s) 247, 446, 654
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 1 cut(s) 90
BslFI GGGAC 1 cut(s) 644
BslI CCNNNNNNNGG 1 cut(s) 687
BsmFI GGGAC 1 cut(s) 644
BsnI GGCC 3 cut(s) 87, 94, 691
BsoBI CYCGRG 1 cut(s) 89
Bsp120I GGGCCC 1 cut(s) 92
Bsp1286I GDGCHC 4 cut(s) 96, 247, 446, 654
Bsp143I GATC 5 cut(s) 127, 133, 199, 269, 376
Bsp68I TCGCGA 1 cut(s) 132
BspACI CCGC 1 cut(s) 79
BspANI GGCC 3 cut(s) 87, 94, 691
BspCNI CTCAG 1 cut(s) 199
BspDI ATCGAT 1 cut(s) 198
BspFNI CGCG 1 cut(s) 132
BspLI GGNNCC 4 cut(s) 88, 94, 95, 307
BspPI GGATC 1 cut(s) 194
BspQI GCTCTTC 1 cut(s) 322
BspT107I GGYRCC 1 cut(s) 305
BspTI CTTAAG 1 cut(s) 337
BsrI ACTGG 1 cut(s) 365
BssECI CCNNGG 4 cut(s) 82, 88, 89, 666
BssMI GATC 5 cut(s) 127, 133, 199, 269, 376
BssT1I CCWWGG 2 cut(s) 82, 666
Bst2UI CCWGG 2 cut(s) 59, 500
Bst4CI ACNGT 2 cut(s) 210, 622
Bst6I CTCTTC 1 cut(s) 322
BstAFI CTTAAG 1 cut(s) 337
BstDEI CTNAG 3 cut(s) 186, 300, 423
BstFNI CGCG 1 cut(s) 132
BstKTI GATC 5 cut(s) 130, 136, 202, 272, 379
BstMBI GATC 5 cut(s) 127, 133, 199, 269, 376
BstMWI GCNNNNNNNGC 3 cut(s) 32, 41, 441
BstNI CCWGG 2 cut(s) 59, 500
BstPAI GACNNNNGTC 1 cut(s) 105
BstSCI CCNGG 4 cut(s) 57, 88, 89, 498
BstSLI GKGCMC 2 cut(s) 96, 247
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 1 cut(s) 382
BstV2I GAAGAC 1 cut(s) 468
BstX2I RGATCY 1 cut(s) 269
BstYI RGATCY 1 cut(s) 269
Bsu15I ATCGAT 1 cut(s) 198
BsuI GTATCC 2 cut(s) 357, 673
BsuRI GGCC 3 cut(s) 87, 94, 691
BsuTUI ATCGAT 1 cut(s) 198
BtrI CACGTC 1 cut(s) 634
BtsI GCAGTG 1 cut(s) 526
BtsIMutI CAGTG 1 cut(s) 526
BtuMI TCGCGA 1 cut(s) 132
Cfr13I GGNCC 5 cut(s) 61, 76, 86, 92, 93
Cfr9I CCCGGG 1 cut(s) 89
ClaI ATCGAT 1 cut(s) 198
CsiI ACCWGGT 1 cut(s) 498
Csp6I GTAC 2 cut(s) 306, 416
CviAII CATG 3 cut(s) 170, 284, 723
CviQI GTAC 2 cut(s) 306, 416
DdeI CTNAG 3 cut(s) 186, 300, 423
DpnI GATC 5 cut(s) 129, 135, 201, 271, 378
DpnII GATC 5 cut(s) 127, 133, 199, 269, 376
Eam1104I CTCTTC 1 cut(s) 322
EarI CTCTTC 1 cut(s) 322
EciI GGCGGA 1 cut(s) 68
Ecl136II GAGCTC 1 cut(s) 444
Eco130I CCWWGG 2 cut(s) 82, 666
Eco24I GRGCYC 2 cut(s) 96, 446
Eco47I GGWCC 2 cut(s) 61, 76
Eco53kI GAGCTC 1 cut(s) 444
Eco57I CTGAAG 1 cut(s) 432
Eco88I CYCGRG 1 cut(s) 89
EcoICRI GAGCTC 1 cut(s) 444
EcoO109I RGGNCCY 2 cut(s) 86, 93
EcoRII CCWGG 2 cut(s) 57, 498
EcoT14I CCWWGG 2 cut(s) 82, 666
EcoT38I GRGCYC 2 cut(s) 96, 446
ErhI CCWWGG 2 cut(s) 82, 666
FaeI CATG 3 cut(s) 173, 287, 726
FaiI YATR 7 cut(s) 74, 171, 281, 285, 381, 596, 724
FaqI GGGAC 1 cut(s) 644
FatI CATG 3 cut(s) 169, 283, 722
FbaI TGATCA 1 cut(s) 376
Fnu4HI GCNGC 1 cut(s) 396
FriOI GRGCYC 2 cut(s) 96, 446
Fsp4HI GCNGC 1 cut(s) 396
FspBI CTAG 3 cut(s) 54, 240, 429
GluI GCNGC 1 cut(s) 396
GsaI CCCAGC 1 cut(s) 685
GsuI CTGGAG 1 cut(s) 15
HaeIII GGCC 3 cut(s) 87, 94, 691
HapII CCGG 1 cut(s) 90
Hin1II CATG 3 cut(s) 173, 287, 726
HinfI GANTC 2 cut(s) 344, 385
HpaII CCGG 1 cut(s) 90
HphI GGTGA 5 cut(s) 158, 488, 634, 682, 702
Hpy166II GTNNAC 1 cut(s) 245
Hpy188III TCNNGA 4 cut(s) 125, 131, 410, 604
Hpy8I GTNNAC 1 cut(s) 245
HpyAV CCTTC 4 cut(s) 361, 536, 569, 702
HpyCH4III ACNGT 2 cut(s) 210, 622
HpyCH4IV ACGT 2 cut(s) 67, 633
HpyCH4V TGCA 4 cut(s) 26, 245, 526, 531
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 41, 441
HpyF3I CTNAG 3 cut(s) 186, 300, 423
HpySE526I ACGT 2 cut(s) 67, 633
Hsp92II CATG 3 cut(s) 173, 287, 726
KpnI GGTACC 1 cut(s) 309
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 5 cut(s) 127, 133, 199, 269, 376
LguI GCTCTTC 1 cut(s) 322
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 1 cut(s) 35
MabI ACCWGGT 1 cut(s) 498
MaeI CTAG 3 cut(s) 54, 240, 429
MaeII ACGT 2 cut(s) 67, 633
MaeIII GTNAC 6 cut(s) 189, 341, 494, 580, 616, 634
MalI GATC 5 cut(s) 129, 135, 201, 271, 378
MboI GATC 5 cut(s) 127, 133, 199, 269, 376
MboII GAAGA 7 cut(s) 113, 309, 312, 315, 468, 471, 685
MflI RGATCY 1 cut(s) 269
MhlI GDGCHC 4 cut(s) 96, 247, 446, 654
MlyI GAGTC 1 cut(s) 338
MnlI CCTC 6 cut(s) 162, 417, 425, 430, 572, 724
MseI TTAA 3 cut(s) 290, 338, 512
MslI CAYNNNNRTG 3 cut(s) 123, 623, 721
MspCI CTTAAG 1 cut(s) 337
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 4 cut(s) 59, 90, 91, 500
MvaI CCWGG 2 cut(s) 59, 500
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 3 cut(s) 32, 41, 441
NciI CCSGG 2 cut(s) 90, 91
NdeII GATC 5 cut(s) 127, 133, 199, 269, 376
NlaIII CATG 3 cut(s) 173, 287, 726
NlaIV GGNNCC 4 cut(s) 88, 94, 95, 307
NmeAIII GCCGAG 1 cut(s) 371
NmuCI GTSAC 5 cut(s) 341, 494, 580, 616, 634
NruI TCGCGA 1 cut(s) 132
PciSI GCTCTTC 1 cut(s) 322
PfeI GAWTC 1 cut(s) 385
PkrI GCNGC 1 cut(s) 397
PleI GAGTC 1 cut(s) 338
PpsI GAGTC 1 cut(s) 338
PshAI GACNNNNGTC 1 cut(s) 105
Psp124BI GAGCTC 1 cut(s) 446
Psp6I CCWGG 2 cut(s) 57, 498
PspFI CCCAGC 1 cut(s) 681
PspGI CCWGG 2 cut(s) 57, 498
PspN4I GGNNCC 4 cut(s) 88, 94, 95, 307
PspOMI GGGCCC 1 cut(s) 92
PspPI GGNCC 5 cut(s) 61, 76, 86, 92, 93
PsuI RGATCY 1 cut(s) 269
RruI TCGCGA 1 cut(s) 132
RsaI GTAC 2 cut(s) 307, 417
RsaNI GTAC 2 cut(s) 306, 416
RseI CAYNNNNRTG 3 cut(s) 123, 623, 721
SacI GAGCTC 1 cut(s) 446
SapI GCTCTTC 1 cut(s) 322
SaqAI TTAA 3 cut(s) 290, 338, 512
SatI GCNGC 1 cut(s) 396
Sau3AI GATC 5 cut(s) 127, 133, 199, 269, 376
Sau96I GGNCC 5 cut(s) 61, 76, 86, 92, 93
SchI GAGTC 1 cut(s) 338
ScrFI CCNGG 4 cut(s) 59, 90, 91, 500
SduI GDGCHC 4 cut(s) 96, 247, 446, 654
SexAI ACCWGGT 1 cut(s) 498
SfaNI GCATC 1 cut(s) 35
SinI GGWCC 2 cut(s) 61, 76
SmaI CCCGGG 1 cut(s) 91
SmiMI CAYNNNNRTG 3 cut(s) 123, 623, 721
SmlI CTYRAG 2 cut(s) 337, 655
SmoI CTYRAG 2 cut(s) 337, 655
SsiI CCGC 1 cut(s) 79
SspMI CTAG 3 cut(s) 54, 240, 429
SstI GAGCTC 1 cut(s) 446
StyD4I CCNGG 4 cut(s) 57, 88, 89, 498
StyI CCWWGG 2 cut(s) 82, 666
TaaI ACNGT 2 cut(s) 210, 622
TaiI ACGT 2 cut(s) 70, 636
TaqI TCGA 2 cut(s) 198, 357
TfiI GAWTC 1 cut(s) 385
Tru1I TTAA 3 cut(s) 290, 338, 512
Tru9I TTAA 3 cut(s) 290, 338, 512
TscAI CASTG 1 cut(s) 533
TseFI GTSAC 5 cut(s) 341, 494, 580, 616, 634
TseI GCWGC 1 cut(s) 395
Tsp45I GTSAC 5 cut(s) 341, 494, 580, 616, 634
TspDTI ATGAA 3 cut(s) 17, 343, 576
TspMI CCCGGG 1 cut(s) 89
TspRI CASTG 1 cut(s) 533
Vha464I CTTAAG 1 cut(s) 337
VneI GTGCAC 1 cut(s) 243
VpaK11BI GGWCC 2 cut(s) 61, 76
XmaI CCCGGG 1 cut(s) 89
XspI CTAG 3 cut(s) 54, 240, 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.