Rorug05G0350000

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009518
Physical Location & Seq
Forward (+)
43458037 .. 43459832
1796 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0350000.1

Sequence Viewer

Length: 981 bp
ATGAATAGCCTGTATTTGGAGGCTAATGTTAACTTTACGGGAAAGAAGAAATCCACCGTTACTGAAGCTGAGGGAAGTGGAAAAATAAAGGCCACATGGCCTGATGAAATAGTAGCAACATTTTGTGATATAGTTGTTAAGGAAGTGGCAAAGGGAAACAGGCCAGGTACTCATTTTGCTGATAAAAAGAGATGGTCTAATGTTGTTGAAGCCTTTAAAAAGTTAACCGGAAGGGACTATGATAAAAGGCAATTGAAAAACAAGTGGGATTCGCTTAAAAATGATTGGAAATTGTGGAGTTCTCTATTGCATAAGGAAACTAGTATTGGATGGGATCCTTCTAAGAAGACCGTTGATGCACCTCCTGAATGGTGGCAAAGCAAAATTCAGGAAAATCCAGAGTATCTCAAATTTCGCGACATGGGAATTTTTCCTGACATGATGGACATTTATGATAAGATGTTCAAGGGTAGCTCAGCTCTAGGCCATTGTGTCATGATTCCTTCAACTAGTATAGATGTTGAAGAGGTTGTGGAGGATTCTGAGTTTAATGTTATTTCTGCTGATGATGAGGAGGATCCTGAGCTAGGTAATGAAGATAGGGGGAAAAAGAGAACAAATGAGGACAGACCAGCAAAAGCTAATAAGGACAAAGGAGTTATGAGAGGGCCAAAGGGGAAGAAAGCAAAGGTGGGAGGTGCAGTGAAATTGTCTAAACAAATTTATCTTCTTGTTGAGGTAGTTGAGAGTAGGAGTACAGCGACTTCAGTTCACACAAATTCACAAGGAACTAGTATTCAGGAGGTGATGCAAGTTGTTGCAAGTTTACCAGGAGCAGAAACTGGTACCAAGCTGTGGTGGTTTGCAACAGAGTTGTTTTGCTCTCAAGAGAAGCAAGAGATGTTTTCAGTTATGACAGATCCTGATTTTAAGCTGCAGTTTCTGATTCTTAATCAGAAAAAGGCTGATTCCACTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

326

Amino Acids

36.83

Weight (kDa)

6.49

Isoelectric Point (pI)

30.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_3 PF12776 33 - 126 2.6e-21 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 845
AccB1I GGYRCC 1 cut(s) 845
AccB7I CCANNNNNTGG 1 cut(s) 855
AccII CGCG 1 cut(s) 417
AclWI GGATC 5 cut(s) 329, 342, 572, 585, 914
AcsI RAATTY 5 cut(s) 384, 410, 426, 720, 778
AcuI CTGAAG 2 cut(s) 84, 750
AfaI GTAC 3 cut(s) 169, 757, 847
AfiI CCNNNNNNNGG 3 cut(s) 16, 587, 855
AgsI TTSAA 5 cut(s) 209, 256, 466, 507, 524
AhlI ACTAGT 3 cut(s) 320, 509, 791
AjnI CCWGG 2 cut(s) 163, 829
AjuI GAANNNNNNNTTGG 2 cut(s) 309, 341
AluBI AGCT 7 cut(s) 68, 474, 479, 586, 641, 853, 934
AluI AGCT 7 cut(s) 68, 474, 479, 586, 641, 853, 934
AlwI GGATC 5 cut(s) 329, 342, 572, 585, 914
AlwNI CAGNNNCTG 3 cut(s) 842, 923, 943
AoxI GGCC 5 cut(s) 90, 98, 161, 484, 668
ApeKI GCWGC 1 cut(s) 934
ApoI RAATTY 5 cut(s) 384, 410, 426, 720, 778
Asp718I GGTACC 1 cut(s) 845
AspS9I GGNCC 1 cut(s) 668
AsuHPI GGTGA 1 cut(s) 817
BamHI GGATCC 2 cut(s) 334, 577
BanI GGYRCC 1 cut(s) 845
BarI GAAGNNNNNNTAC 2 cut(s) 748, 780
BbsI GAAGAC 1 cut(s) 353
BbvCI CCTCAGC 1 cut(s) 69
BbvI GCAGC 1 cut(s) 921
BccI CCATC 3 cut(s) 186, 324, 436
BciT130I CCWGG 2 cut(s) 165, 831
BcuI ACTAGT 3 cut(s) 320, 509, 791
BfaI CTAG 5 cut(s) 321, 482, 510, 587, 792
BfmI CTRYAG 1 cut(s) 935
BisI GCNGC 1 cut(s) 935
BlpI GCTNAGC 1 cut(s) 475
BlsI GCNGC 1 cut(s) 936
Bme1390I CCNGG 2 cut(s) 165, 831
BmgT120I GGNCC 1 cut(s) 668
BmiI GGNNCC 3 cut(s) 336, 579, 847
BmrFI CCNGG 2 cut(s) 165, 831
BmsI GCATC 2 cut(s) 346, 798
BpiI GAAGAC 1 cut(s) 353
Bpu10I CCTNAGC 2 cut(s) 69, 582
Bpu1102I GCTNAGC 1 cut(s) 475
BpuEI CTTGAG 1 cut(s) 870
BsaWI WCCGGW 1 cut(s) 227
BsaXI ACNNNNNCTCC 2 cut(s) 687, 717
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 587, 855
Bse1I ACTGG 1 cut(s) 847
BseBI CCWGG 2 cut(s) 165, 831
BseGI GGATG 1 cut(s) 335
BseLI CCNNNNNNNGG 3 cut(s) 16, 587, 855
BseMII CTCAG 4 cut(s) 60, 489, 534, 573
BseNI ACTGG 1 cut(s) 847
BseRI GAGGAG 1 cut(s) 587
BseXI GCAGC 1 cut(s) 921
BsgI GTGCAG 1 cut(s) 720
Bsh1236I CGCG 1 cut(s) 417
BshFI GGCC 5 cut(s) 92, 100, 163, 486, 670
BshNI GGYRCC 1 cut(s) 845
BsiSI CCGG 1 cut(s) 228
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 3 cut(s) 16, 587, 855
BsmFI GGGAC 1 cut(s) 248
BsnI GGCC 5 cut(s) 92, 100, 163, 486, 670
Bsp143I GATC 3 cut(s) 334, 577, 919
Bsp1720I GCTNAGC 1 cut(s) 475
Bsp68I TCGCGA 1 cut(s) 417
BspANI GGCC 5 cut(s) 92, 100, 163, 486, 670
BspCNI CTCAG 4 cut(s) 61, 488, 535, 574
BspFNI CGCG 1 cut(s) 417
BspHI TCATGA 1 cut(s) 495
BspLI GGNNCC 3 cut(s) 336, 579, 847
BspMAI CTGCAG 1 cut(s) 939
BspPI GGATC 5 cut(s) 329, 342, 572, 585, 914
BspT107I GGYRCC 1 cut(s) 845
BsrI ACTGG 1 cut(s) 847
BssMI GATC 3 cut(s) 334, 577, 919
Bst2UI CCWGG 2 cut(s) 165, 831
Bst4CI ACNGT 2 cut(s) 58, 352
Bst6I CTCTTC 1 cut(s) 519
BstDEI CTNAG 5 cut(s) 69, 342, 475, 543, 582
BstENI CCTNNNNNAGG 1 cut(s) 585
BstF5I GGATG 1 cut(s) 335
BstFNI CGCG 1 cut(s) 417
BstKTI GATC 3 cut(s) 337, 580, 922
BstMBI GATC 3 cut(s) 334, 577, 919
BstNI CCWGG 2 cut(s) 165, 831
BstSCI CCNGG 2 cut(s) 163, 829
BstSFI CTRYAG 1 cut(s) 935
BstUI CGCG 1 cut(s) 417
BstV1I GCAGC 1 cut(s) 921
BstV2I GAAGAC 1 cut(s) 353
BstX2I RGATCY 3 cut(s) 334, 577, 919
BstYI RGATCY 3 cut(s) 334, 577, 919
BsuRI GGCC 5 cut(s) 92, 100, 163, 486, 670
BtsCI GGATG 1 cut(s) 335
BtsI GCAGTG 1 cut(s) 708
BtsIMutI CAGTG 1 cut(s) 708
BtuMI TCGCGA 1 cut(s) 417
CaiI CAGNNNCTG 3 cut(s) 842, 923, 943
CciI TCATGA 1 cut(s) 495
Cfr13I GGNCC 1 cut(s) 668
Csp6I GTAC 3 cut(s) 168, 756, 846
CviAII CATG 4 cut(s) 96, 421, 439, 496
CviQI GTAC 3 cut(s) 168, 756, 846
DdeI CTNAG 5 cut(s) 69, 342, 475, 543, 582
DpnI GATC 3 cut(s) 336, 579, 921
DpnII GATC 3 cut(s) 334, 577, 919
DraI TTTAAA 1 cut(s) 217
Eam1104I CTCTTC 1 cut(s) 519
EarI CTCTTC 1 cut(s) 519
Eco57I CTGAAG 2 cut(s) 84, 750
EcoNI CCTNNNNNAGG 1 cut(s) 585
EcoRII CCWGG 2 cut(s) 163, 829
FaeI CATG 4 cut(s) 99, 424, 442, 499
FaqI GGGAC 1 cut(s) 248
FatI CATG 4 cut(s) 95, 420, 438, 495
Fnu4HI GCNGC 1 cut(s) 935
FokI GGATG 1 cut(s) 342
Fsp4HI GCNGC 1 cut(s) 935
FspBI CTAG 5 cut(s) 321, 482, 510, 587, 792
GluI GCNGC 1 cut(s) 935
HaeIII GGCC 5 cut(s) 92, 100, 163, 486, 670
HapII CCGG 1 cut(s) 228
Hin1II CATG 4 cut(s) 99, 424, 442, 499
HincII GTYRAC 2 cut(s) 31, 225
HindII GTYRAC 2 cut(s) 31, 225
HinfI GANTC 5 cut(s) 269, 499, 539, 946, 968
HpaI GTTAAC 2 cut(s) 31, 225
HpaII CCGG 1 cut(s) 228
HphI GGTGA 1 cut(s) 817
Hpy166II GTNNAC 4 cut(s) 31, 225, 772, 827
Hpy188I TCNGA 3 cut(s) 544, 945, 957
Hpy8I GTNNAC 4 cut(s) 31, 225, 772, 827
HpyAV CCTTC 3 cut(s) 225, 348, 513
HpyCH4III ACNGT 2 cut(s) 58, 352
HpyCH4V TGCA 7 cut(s) 310, 359, 701, 811, 821, 866, 937
HpyF3I CTNAG 5 cut(s) 69, 342, 475, 543, 582
Hsp92II CATG 4 cut(s) 99, 424, 442, 499
KpnI GGTACC 1 cut(s) 849
KspAI GTTAAC 2 cut(s) 31, 225
Kzo9I GATC 3 cut(s) 334, 577, 919
LmnI GCTCC 1 cut(s) 833
Lsp1109I GCAGC 1 cut(s) 921
LweI GCATC 2 cut(s) 346, 798
MaeI CTAG 5 cut(s) 321, 482, 510, 587, 792
MaeIII GTNAC 1 cut(s) 58
MalI GATC 3 cut(s) 336, 579, 921
MboI GATC 3 cut(s) 334, 577, 919
MboII GAAGA 6 cut(s) 58, 358, 536, 608, 691, 719
MfeI CAATTG 1 cut(s) 251
MflI RGATCY 3 cut(s) 334, 577, 919
MluCI AATT 8 cut(s) 251, 290, 384, 410, 426, 707, 720, 778
MseI TTAA 8 cut(s) 30, 138, 216, 224, 276, 549, 930, 951
MspI CCGG 1 cut(s) 228
MspR9I CCNGG 2 cut(s) 165, 831
MunI CAATTG 1 cut(s) 251
MvaI CCWGG 2 cut(s) 165, 831
MvnI CGCG 1 cut(s) 417
NdeII GATC 3 cut(s) 334, 577, 919
NlaIII CATG 4 cut(s) 99, 424, 442, 499
NlaIV GGNNCC 3 cut(s) 336, 579, 847
NruI TCGCGA 1 cut(s) 417
PagI TCATGA 1 cut(s) 495
PfeI GAWTC 5 cut(s) 269, 499, 539, 946, 968
PflMI CCANNNNNTGG 1 cut(s) 855
PkrI GCNGC 1 cut(s) 936
Psp6I CCWGG 2 cut(s) 163, 829
PspGI CCWGG 2 cut(s) 163, 829
PspN4I GGNNCC 3 cut(s) 336, 579, 847
PspPI GGNCC 1 cut(s) 668
PstI CTGCAG 1 cut(s) 939
PstNI CAGNNNCTG 3 cut(s) 842, 923, 943
PsuI RGATCY 3 cut(s) 334, 577, 919
RruI TCGCGA 1 cut(s) 417
RsaI GTAC 3 cut(s) 169, 757, 847
RsaNI GTAC 3 cut(s) 168, 756, 846
SaqAI TTAA 8 cut(s) 30, 138, 216, 224, 276, 549, 930, 951
SatI GCNGC 1 cut(s) 935
Sau3AI GATC 3 cut(s) 334, 577, 919
Sau96I GGNCC 1 cut(s) 668
ScrFI CCNGG 2 cut(s) 165, 831
SfaNI GCATC 2 cut(s) 346, 798
SfcI CTRYAG 1 cut(s) 935
SmlI CTYRAG 1 cut(s) 885
SmoI CTYRAG 1 cut(s) 885
SpeI ACTAGT 3 cut(s) 320, 509, 791
Sse9I AATT 8 cut(s) 251, 290, 384, 410, 426, 707, 720, 778
SspMI CTAG 5 cut(s) 321, 482, 510, 587, 792
StyD4I CCNGG 2 cut(s) 163, 829
TaaI ACNGT 2 cut(s) 58, 352
TasI AATT 8 cut(s) 251, 290, 384, 410, 426, 707, 720, 778
TatI WGTACW 1 cut(s) 755
TfiI GAWTC 5 cut(s) 269, 499, 539, 946, 968
Tru1I TTAA 8 cut(s) 30, 138, 216, 224, 276, 549, 930, 951
Tru9I TTAA 8 cut(s) 30, 138, 216, 224, 276, 549, 930, 951
TscAI CASTG 1 cut(s) 708
TseI GCWGC 1 cut(s) 934
TspDTI ATGAA 3 cut(s) 17, 120, 609
TspRI CASTG 1 cut(s) 708
Van91I CCANNNNNTGG 1 cut(s) 855
XagI CCTNNNNNAGG 1 cut(s) 585
XapI RAATTY 5 cut(s) 384, 410, 426, 720, 778
XspI CTAG 5 cut(s) 321, 482, 510, 587, 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.