FvH4_3g37161

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016808
Physical Location & Seq
Forward (+)
31843323 .. 31844731
1409 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g37161.t1

Sequence Viewer

Length: 1059 bp
ATGACACGGGTCATGGAACCCACACTTGCCTCAACGGCAGCAGGAAATGCTACAAGAGATGTAAGCTTTTATGGACTAGCACAAGGTACTGCAAGAGGAGGAGTTCCCTCTGCAAGAATTGCTGCATACAAAGTCTGTACTGCAGGCACATTGTGTAGCGCACATGATACCTTGGCTGCTTTTGATGATGCAATTGCTGATGGAGTTGATATTATTACAGCTTCAGTTGCATTTAGTATGATCCAAGAATTTGATGTGGATCCTCTTGCAATTGGTACTTTTCATGCGATGGAGAAAGGGATACTAACCTCACATGCTGCAGGCAACAATGGACCTTCCGGTGCTACTGTATCAAGTGTAGCACCTTGGGTGCTTACAGTTGCAGCCAGTAGCATGGACCAGCGGATCATTGACAAGGTTGTCCTTGGAAACGAAACCACAGTAATTGGGAATTCTGTGAACTCTTTCACATTAAATGGTACAAGTTTTCCTTTGATACATGGAAAAGATGCTTCAAGGAATTGCACAGGAACAGAAGCAAGAGATTGTGAACAAGGTTACCTGGACAGTGATCTAGTGAAGGGAAGGGTTCTCCTATGCGATAGATACAGACTTCTTGAAGCATTTCAATCTGGTGCACTGGGTTCCATTTTGCCCAAGTTTCGAGTTGTTGATGATGCCTCTTATGTTCTCCCATTAGCTGGCATGACTATAAACAACGAAAACTATAATGTAGTGATGTCCTACATGAACTCCACCAGAGATCCTCGTGTGAACATATTAAAAAGTGAAGTCATCAAAAATCCTGCTGCACCTGCTGTTGCTTCATTCTCCGCACGTGGACCAAATCTAATTTTACCTGATATTATTAAGCCAGATACAAGGCCATCTCCAGTAATAGAGGCTATAGAGCTAAATCCTAAATTTTGGCCTTATTTTGATCTCCAACAAGAACTTAGAAGAGGGTCAAAGGGCCATAAAAGGGCCAAAGGGCCATCAAAAGGGCCAAACATAGCCTTTTGGCTAGCCTGGGCCAAACCAAATTGTGGTCCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

353

Amino Acids

37.55

Weight (kDa)

7.6

Isoelectric Point (pI)

32.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 9 - 292 4.1e-13 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 823
AasI GACNNNNNNGTC 1 cut(s) 419
Acc36I ACCTGC 1 cut(s) 823
AccB7I CCANNNNNTGG 2 cut(s) 701, 1046
AciI CCGC 2 cut(s) 403, 834
AclWI GGATC 5 cut(s) 235, 254, 267, 413, 758
AcsI RAATTY 3 cut(s) 248, 451, 923
AcuI CTGAAG 1 cut(s) 207
AcvI CACGTG 1 cut(s) 839
AdeI CACNNNGTG 1 cut(s) 153
AfaI GTAC 4 cut(s) 88, 139, 277, 481
AfiI CCNNNNNNNGG 4 cut(s) 701, 982, 1001, 1046
AgsI TTSAA 3 cut(s) 516, 620, 629
AjnI CCWGG 2 cut(s) 561, 1028
AluBI AGCT 4 cut(s) 66, 221, 701, 913
AluI AGCT 4 cut(s) 66, 221, 701, 913
Alw21I GWGCWC 1 cut(s) 640
Alw44I GTGCAC 1 cut(s) 636
AlwI GGATC 5 cut(s) 235, 254, 267, 413, 758
AoxI GGCC 7 cut(s) 884, 929, 973, 984, 992, 1004, 1032
ApaLI GTGCAC 1 cut(s) 636
ApeKI GCWGC 6 cut(s) 38, 122, 176, 317, 383, 809
ApoI RAATTY 3 cut(s) 248, 451, 923
Asp700I GAANNNNTTC 2 cut(s) 464, 624
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 9 cut(s) 332, 397, 842, 973, 984, 992, 1004, 1032, 1049
AsuNHI GCTAGC 1 cut(s) 1024
AvaII GGWCC 4 cut(s) 332, 397, 842, 1049
BaeGI GKGCMC 1 cut(s) 640
BamHI GGATCC 1 cut(s) 259
BauI CACGAG 1 cut(s) 768
BbrPI CACGTG 1 cut(s) 839
Bbv12I GWGCWC 1 cut(s) 640
BbvI GCAGC 6 cut(s) 50, 109, 163, 304, 395, 796
BccI CCATC 4 cut(s) 194, 283, 895, 1003
BceAI ACGGC 1 cut(s) 51
BciT130I CCWGG 2 cut(s) 563, 1030
BciVI GTATCC 1 cut(s) 294
BfaI CTAG 3 cut(s) 77, 575, 1025
BfmI CTRYAG 3 cut(s) 141, 318, 906
BfuAI ACCTGC 1 cut(s) 823
BfuI GTATCC 1 cut(s) 294
BglI GCCNNNNNGGC 1 cut(s) 35
BisI GCNGC 6 cut(s) 39, 123, 177, 318, 384, 810
BlsI GCNGC 6 cut(s) 40, 124, 178, 319, 385, 811
Bme1390I CCNGG 2 cut(s) 563, 1030
Bme18I GGWCC 4 cut(s) 332, 397, 842, 1049
BmgT120I GGNCC 9 cut(s) 332, 397, 842, 973, 984, 992, 1004, 1032, 1049
BmiI GGNNCC 4 cut(s) 18, 261, 646, 1051
BmrFI CCNGG 2 cut(s) 563, 1030
BmrI ACTGGG 1 cut(s) 650
BmsI GCATC 3 cut(s) 178, 499, 667
BmtI GCTAGC 1 cut(s) 1028
BmuI ACTGGG 1 cut(s) 650
BoxI GACNNNNGTC 1 cut(s) 8
BpmI CTGGAG 1 cut(s) 876
BsaAI YACGTR 1 cut(s) 839
BsaBI GATNNNNATC 1 cut(s) 258
BsaJI CCNNGG 4 cut(s) 171, 365, 424, 1029
BsaWI WCCGGW 1 cut(s) 338
BsaXI ACNNNNNCTCC 2 cut(s) 737, 767
Bsc4I CCNNNNNNNGG 4 cut(s) 701, 982, 1001, 1046
Bse1I ACTGG 3 cut(s) 387, 645, 893
Bse8I GATNNNNATC 1 cut(s) 258
BseBI CCWGG 2 cut(s) 563, 1030
BseDI CCNNGG 4 cut(s) 171, 365, 424, 1029
BseJI GATNNNNATC 1 cut(s) 258
BseLI CCNNNNNNNGG 4 cut(s) 701, 982, 1001, 1046
BseNI ACTGG 3 cut(s) 387, 645, 893
BseRI GAGGAG 2 cut(s) 111, 114
BseSI GKGCMC 1 cut(s) 640
BseXI GCAGC 6 cut(s) 50, 109, 163, 304, 395, 796
BsgI GTGCAG 1 cut(s) 795
BshFI GGCC 7 cut(s) 886, 931, 975, 986, 994, 1006, 1034
BsiHKAI GWGCWC 1 cut(s) 640
BsiSI CCGG 1 cut(s) 339
BslFI GGGAC 1 cut(s) 1035
BslI CCNNNNNNNGG 4 cut(s) 701, 982, 1001, 1046
BsmFI GGGAC 1 cut(s) 1035
BsnI GGCC 7 cut(s) 886, 931, 975, 986, 994, 1006, 1034
Bsp1286I GDGCHC 1 cut(s) 640
Bsp143I GATC 6 cut(s) 240, 259, 405, 571, 763, 940
BspACI CCGC 2 cut(s) 403, 834
BspANI GGCC 7 cut(s) 886, 931, 975, 986, 994, 1006, 1034
BspLI GGNNCC 4 cut(s) 18, 261, 646, 1051
BspMAI CTGCAG 2 cut(s) 145, 322
BspMI ACCTGC 1 cut(s) 823
BspOI GCTAGC 1 cut(s) 1028
BspPI GGATC 5 cut(s) 235, 254, 267, 413, 758
BsrI ACTGG 3 cut(s) 387, 645, 893
BssECI CCNNGG 4 cut(s) 171, 365, 424, 1029
BssMI GATC 6 cut(s) 240, 259, 405, 571, 763, 940
BssSI CACGAG 1 cut(s) 768
BssT1I CCWWGG 3 cut(s) 171, 365, 424
Bst2BI CACGAG 1 cut(s) 768
Bst2UI CCWGG 2 cut(s) 563, 1030
Bst4CI ACNGT 4 cut(s) 349, 379, 442, 569
Bst6I CTCTTC 1 cut(s) 955
BstAPI GCANNNNNTGC 2 cut(s) 47, 119
BstBAI YACGTR 1 cut(s) 839
BstC8I GCNNGC 4 cut(s) 145, 322, 703, 1026
BstDEI CTNAG 1 cut(s) 956
BstEII GGTNACC 1 cut(s) 557
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 6 cut(s) 243, 262, 408, 574, 766, 943
BstMBI GATC 6 cut(s) 240, 259, 405, 571, 763, 940
BstMWI GCNNNNNNNGC 5 cut(s) 35, 47, 119, 227, 815
BstNI CCWGG 2 cut(s) 563, 1030
BstNSI RCATGY 1 cut(s) 317
BstPAI GACNNNNGTC 1 cut(s) 8
BstPI GGTNACC 1 cut(s) 557
BstSCI CCNGG 2 cut(s) 561, 1028
BstSFI CTRYAG 3 cut(s) 141, 318, 906
BstSLI GKGCMC 1 cut(s) 640
BstV1I GCAGC 6 cut(s) 50, 109, 163, 304, 395, 796
BstX2I RGATCY 2 cut(s) 259, 763
BstXI CCANNNNNNTGG 1 cut(s) 394
BstYI RGATCY 2 cut(s) 259, 763
BsuI GTATCC 1 cut(s) 294
BsuRI GGCC 7 cut(s) 886, 931, 975, 986, 994, 1006, 1034
BtgZI GCGATG 1 cut(s) 302
BtsIMutI CAGTG 2 cut(s) 574, 638
BveI ACCTGC 1 cut(s) 823
Cac8I GCNNGC 4 cut(s) 145, 322, 703, 1026
CfoI GCGC 1 cut(s) 161
Cfr13I GGNCC 9 cut(s) 332, 397, 842, 973, 984, 992, 1004, 1032, 1049
Csp6I GTAC 4 cut(s) 87, 138, 276, 480
CspCI CAANNNNNGTGG 2 cut(s) 427, 462
CviAII CATG 8 cut(s) 13, 164, 284, 314, 394, 500, 706, 748
CviQI GTAC 4 cut(s) 87, 138, 276, 480
DdeI CTNAG 1 cut(s) 956
DpnI GATC 6 cut(s) 242, 261, 407, 573, 765, 942
DpnII GATC 6 cut(s) 240, 259, 405, 571, 763, 940
DraIII CACNNNGTG 1 cut(s) 153
DrdI GACNNNNNNGTC 1 cut(s) 419
DseDI GACNNNNNNGTC 1 cut(s) 419
Eam1104I CTCTTC 1 cut(s) 955
EarI CTCTTC 1 cut(s) 955
Eco130I CCWWGG 3 cut(s) 171, 365, 424
Eco47I GGWCC 4 cut(s) 332, 397, 842, 1049
Eco57I CTGAAG 1 cut(s) 207
Eco72I CACGTG 1 cut(s) 839
Eco91I GGTNACC 1 cut(s) 557
EcoO65I GGTNACC 1 cut(s) 557
EcoRI GAATTC 1 cut(s) 451
EcoRII CCWGG 2 cut(s) 561, 1028
EcoT14I CCWWGG 3 cut(s) 171, 365, 424
ErhI CCWWGG 3 cut(s) 171, 365, 424
FaeI CATG 8 cut(s) 16, 167, 287, 317, 397, 503, 709, 751
FalI AAGNNNNNCTT 2 cut(s) 475, 507
FaqI GGGAC 1 cut(s) 1035
FatI CATG 8 cut(s) 12, 163, 283, 313, 393, 499, 705, 747
Fnu4HI GCNGC 6 cut(s) 39, 123, 177, 318, 384, 810
Fsp4HI GCNGC 6 cut(s) 39, 123, 177, 318, 384, 810
FspBI CTAG 3 cut(s) 77, 575, 1025
GlaI GCGC 1 cut(s) 160
GluI GCNGC 6 cut(s) 39, 123, 177, 318, 384, 810
GsuI CTGGAG 1 cut(s) 876
HaeIII GGCC 7 cut(s) 886, 931, 975, 986, 994, 1006, 1034
HapII CCGG 1 cut(s) 339
HhaI GCGC 1 cut(s) 161
Hin1II CATG 8 cut(s) 16, 167, 287, 317, 397, 503, 709, 751
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HindIII AAGCTT 1 cut(s) 64
HpaII CCGG 1 cut(s) 339
Hpy166II GTNNAC 5 cut(s) 460, 551, 638, 775, 842
Hpy188III TCNNGA 1 cut(s) 617
Hpy8I GTNNAC 5 cut(s) 460, 551, 638, 775, 842
HpyAV CCTTC 3 cut(s) 345, 574, 579
HpyCH4III ACNGT 4 cut(s) 349, 379, 442, 569
HpyCH4IV ACGT 1 cut(s) 838
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 47, 119, 227, 815
HpyF3I CTNAG 1 cut(s) 956
HpySE526I ACGT 1 cut(s) 838
Hsp92II CATG 8 cut(s) 16, 167, 287, 317, 397, 503, 709, 751
HspAI GCGC 1 cut(s) 159
Kzo9I GATC 6 cut(s) 240, 259, 405, 571, 763, 940
Lsp1109I GCAGC 6 cut(s) 50, 109, 163, 304, 395, 796
LweI GCATC 3 cut(s) 178, 499, 667
MaeI CTAG 3 cut(s) 77, 575, 1025
MaeII ACGT 1 cut(s) 838
MaeIII GTNAC 1 cut(s) 557
MalI GATC 6 cut(s) 242, 261, 407, 573, 765, 942
MboI GATC 6 cut(s) 240, 259, 405, 571, 763, 940
MboII GAAGA 1 cut(s) 972
MfeI CAATTG 2 cut(s) 192, 270
MflI RGATCY 2 cut(s) 259, 763
MhlI GDGCHC 1 cut(s) 640
MmeI TCCRAC 1 cut(s) 970
MroXI GAANNNNTTC 2 cut(s) 464, 624
MseI TTAA 3 cut(s) 473, 782, 870
MspA1I CMGCKG 1 cut(s) 403
MspI CCGG 1 cut(s) 339
MspR9I CCNGG 2 cut(s) 563, 1030
MunI CAATTG 2 cut(s) 192, 270
MvaI CCWGG 2 cut(s) 563, 1030
MwoI GCNNNNNNNGC 5 cut(s) 35, 47, 119, 227, 815
NdeII GATC 6 cut(s) 240, 259, 405, 571, 763, 940
NheI GCTAGC 1 cut(s) 1024
NlaIII CATG 8 cut(s) 16, 167, 287, 317, 397, 503, 709, 751
NlaIV GGNNCC 4 cut(s) 18, 261, 646, 1051
NspI RCATGY 1 cut(s) 317
PaqCI CACCTGC 1 cut(s) 823
PdmI GAANNNNTTC 2 cut(s) 464, 624
PflMI CCANNNNNTGG 2 cut(s) 701, 1046
PkrI GCNGC 6 cut(s) 40, 124, 178, 319, 385, 811
PmaCI CACGTG 1 cut(s) 839
PmlI CACGTG 1 cut(s) 839
Ppu21I YACGTR 1 cut(s) 839
PshAI GACNNNNGTC 1 cut(s) 8
Psp6I CCWGG 2 cut(s) 561, 1028
PspCI CACGTG 1 cut(s) 839
PspEI GGTNACC 1 cut(s) 557
PspGI CCWGG 2 cut(s) 561, 1028
PspN4I GGNNCC 4 cut(s) 18, 261, 646, 1051
PspPI GGNCC 9 cut(s) 332, 397, 842, 973, 984, 992, 1004, 1032, 1049
PstI CTGCAG 2 cut(s) 145, 322
PsuI RGATCY 2 cut(s) 259, 763
RsaI GTAC 4 cut(s) 88, 139, 277, 481
RsaNI GTAC 4 cut(s) 87, 138, 276, 480
SaqAI TTAA 3 cut(s) 473, 782, 870
SatI GCNGC 6 cut(s) 39, 123, 177, 318, 384, 810
Sau3AI GATC 6 cut(s) 240, 259, 405, 571, 763, 940
Sau96I GGNCC 9 cut(s) 332, 397, 842, 973, 984, 992, 1004, 1032, 1049
ScrFI CCNGG 2 cut(s) 563, 1030
SduI GDGCHC 1 cut(s) 640
SfaNI GCATC 3 cut(s) 178, 499, 667
SfcI CTRYAG 3 cut(s) 141, 318, 906
SinI GGWCC 4 cut(s) 332, 397, 842, 1049
SsiI CCGC 2 cut(s) 403, 834
SspMI CTAG 3 cut(s) 77, 575, 1025
StyD4I CCNGG 2 cut(s) 561, 1028
StyI CCWWGG 3 cut(s) 171, 365, 424
TaaI ACNGT 4 cut(s) 349, 379, 442, 569
TaiI ACGT 1 cut(s) 841
TaqI TCGA 1 cut(s) 664
TatI WGTACW 1 cut(s) 137
Tru1I TTAA 3 cut(s) 473, 782, 870
Tru9I TTAA 3 cut(s) 473, 782, 870
TscAI CASTG 2 cut(s) 574, 645
TseI GCWGC 6 cut(s) 38, 122, 176, 317, 383, 809
TspDTI ATGAA 3 cut(s) 272, 764, 816
TspRI CASTG 2 cut(s) 574, 645
Van91I CCANNNNNTGG 2 cut(s) 701, 1046
VneI GTGCAC 1 cut(s) 636
VpaK11BI GGWCC 4 cut(s) 332, 397, 842, 1049
XapI RAATTY 3 cut(s) 248, 451, 923
XceI RCATGY 1 cut(s) 317
XmnI GAANNNNTTC 2 cut(s) 464, 624
XspI CTAG 3 cut(s) 77, 575, 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.