MD09G1128100.v1.1

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0016808
Physical Location & Seq
Forward (+)
9854499 .. 9856357
1859 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1128100.v1.1.491

Sequence Viewer

Length: 663 bp
ATGGACACTGGAATTTGGCCTGAAGCTGAGAGCTTTAAAGATGAAGGTTTTGGTCCTCCTCCTAAGAAGTGGAAAGGTGTTTGTGAAGGCGGCAAAAATTTCACCTGCAACAAAAAGATCGTTGGTGCTCGATATTACTTAACAGACTCTGCAAGGGATTTCTGGGGTCATGGAACTCACTGTGCATCAATTGTAGCCAGGAGTCTTGTAAAGGATGTAAGCTTTTACGGACTAGCAAATGGTACTGCAAGAGGAGGTGTACCTGCTGCGAGAATCACAGCATATAAAGTCTGTGATCTTGATTTCGGGTGTAGTTCAGACAGTATCATGGCTGCTTTTGACGATGCAATTGCTGATGAAGTTGACATCATTTCAATTTCACTTAATGGATTTTTTCGTTCTGAGCCTCATTTTTATATTGACCCGATTGCAATTGGTGCCTTTCATGCCATGAAGAAAGGGATACTAACTTCACAGTCTGCCGAAACCATATTTAATAGTTATAAGGTTGGTCATGTGTCAAGTGTAGCACCATGGATATTCACAGTTGCAGCTAGTACCACAGATCGCCGGATTATTGACAGCACTGTTCTTGCAAACGGAAAGAAACTAGTCGTATGTCTCATCCACTTCCTTTTTCAACAAAATAAAAACATTTTATGA

Protein Analysis

221

Amino Acids

23.97

Weight (kDa)

7.63

Isoelectric Point (pI)

29.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 45 - 190 1.1e-13 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 504
AarI CACCTGC 1 cut(s) 113
Acc36I ACCTGC 2 cut(s) 113, 271
AccB1I GGYRCC 1 cut(s) 437
AciI CCGC 1 cut(s) 90
AcsI RAATTY 2 cut(s) 12, 97
AcuI CTGAAG 1 cut(s) 42
AfaI GTAC 3 cut(s) 244, 261, 559
AgsI TTSAA 2 cut(s) 375, 641
AhlI ACTAGT 1 cut(s) 610
AjnI CCWGG 1 cut(s) 197
AluBI AGCT 4 cut(s) 26, 33, 222, 554
AluI AGCT 4 cut(s) 26, 33, 222, 554
Alw21I GWGCWC 1 cut(s) 130
Alw26I GTCTC 1 cut(s) 626
AlwNI CAGNNNCTG 1 cut(s) 149
AoxI GGCC 1 cut(s) 17
ApeKI GCWGC 3 cut(s) 266, 332, 551
ApoI RAATTY 2 cut(s) 12, 97
AspS9I GGNCC 1 cut(s) 53
AsuHPI GGTGA 1 cut(s) 94
AvaII GGWCC 1 cut(s) 53
BanI GGYRCC 1 cut(s) 437
Bbv12I GWGCWC 1 cut(s) 130
BbvI GCAGC 3 cut(s) 253, 319, 563
BcgI CGANNNNNNTGC 2 cut(s) 332, 366
BciT130I CCWGG 1 cut(s) 199
BciVI GTATCC 1 cut(s) 456
BcoDI GTCTC 1 cut(s) 626
BcuI ACTAGT 1 cut(s) 610
BfaI CTAG 3 cut(s) 233, 555, 611
BfuAI ACCTGC 2 cut(s) 113, 271
BfuI GTATCC 1 cut(s) 456
BisI GCNGC 4 cut(s) 91, 267, 333, 552
BlsI GCNGC 4 cut(s) 92, 268, 334, 553
Bme1390I CCNGG 1 cut(s) 199
Bme18I GGWCC 1 cut(s) 53
BmgT120I GGNCC 1 cut(s) 53
BmiI GGNNCC 1 cut(s) 439
BmrFI CCNGG 1 cut(s) 199
BmsI GCATC 2 cut(s) 194, 334
BsaJI CCNNGG 1 cut(s) 533
Bse1I ACTGG 1 cut(s) 13
BseBI CCWGG 1 cut(s) 199
BseDI CCNNGG 1 cut(s) 533
BseGI GGATG 2 cut(s) 220, 624
BseMII CTCAG 2 cut(s) 18, 393
BseNI ACTGG 1 cut(s) 13
BseRI GAGGAG 2 cut(s) 48, 267
BseXI GCAGC 3 cut(s) 253, 319, 563
BshFI GGCC 1 cut(s) 19
BshNI GGYRCC 1 cut(s) 437
BsiHKAI GWGCWC 1 cut(s) 130
BsiSI CCGG 1 cut(s) 571
BsmAI GTCTC 1 cut(s) 626
BsnI GGCC 1 cut(s) 19
Bsp1286I GDGCHC 1 cut(s) 130
Bsp143I GATC 3 cut(s) 117, 295, 565
Bsp19I CCATGG 1 cut(s) 533
BspACI CCGC 1 cut(s) 90
BspANI GGCC 1 cut(s) 19
BspCNI CTCAG 2 cut(s) 19, 394
BspLI GGNNCC 1 cut(s) 439
BspMI ACCTGC 2 cut(s) 113, 271
BspT107I GGYRCC 1 cut(s) 437
BsrI ACTGG 1 cut(s) 13
BssECI CCNNGG 1 cut(s) 533
BssMI GATC 3 cut(s) 117, 295, 565
BssT1I CCWWGG 1 cut(s) 533
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 5 cut(s) 182, 323, 477, 547, 589
BstAPI GCANNNNNTGC 1 cut(s) 437
BstDEI CTNAG 3 cut(s) 27, 63, 402
BstDSI CCRYGG 1 cut(s) 533
BstF5I GGATG 2 cut(s) 220, 624
BstKTI GATC 3 cut(s) 120, 298, 568
BstMAI GTCTC 1 cut(s) 626
BstMBI GATC 3 cut(s) 117, 295, 565
BstMWI GCNNNNNNNGC 2 cut(s) 437, 446
BstNI CCWGG 1 cut(s) 199
BstSCI CCNGG 1 cut(s) 197
BstV1I GCAGC 3 cut(s) 253, 319, 563
BsuI GTATCC 1 cut(s) 456
BsuRI GGCC 1 cut(s) 19
BtgI CCRYGG 1 cut(s) 533
BtsCI GGATG 2 cut(s) 220, 624
BtsIMutI CAGTG 3 cut(s) 6, 178, 585
BveI ACCTGC 2 cut(s) 113, 271
CaiI CAGNNNCTG 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 53
Csp6I GTAC 3 cut(s) 243, 260, 558
CviAII CATG 6 cut(s) 170, 328, 446, 451, 515, 534
CviJI RGCY 8 cut(s) 19, 26, 33, 197, 222, 332, 406, 554
CviKI_1 RGCY 8 cut(s) 19, 26, 33, 197, 222, 332, 406, 554
CviQI GTAC 3 cut(s) 243, 260, 558
DdeI CTNAG 3 cut(s) 27, 63, 402
DpnI GATC 3 cut(s) 119, 297, 567
DpnII GATC 3 cut(s) 117, 295, 565
DraI TTTAAA 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 533
Eco47I GGWCC 1 cut(s) 53
Eco57I CTGAAG 1 cut(s) 42
EcoRII CCWGG 1 cut(s) 197
EcoT14I CCWWGG 1 cut(s) 533
ErhI CCWWGG 1 cut(s) 533
FaeI CATG 6 cut(s) 173, 331, 449, 454, 518, 537
FatI CATG 6 cut(s) 169, 327, 445, 450, 514, 533
Fnu4HI GCNGC 4 cut(s) 91, 267, 333, 552
FokI GGATG 2 cut(s) 227, 611
Fsp4HI GCNGC 4 cut(s) 91, 267, 333, 552
FspBI CTAG 3 cut(s) 233, 555, 611
GluI GCNGC 4 cut(s) 91, 267, 333, 552
HaeIII GGCC 1 cut(s) 19
HapII CCGG 1 cut(s) 571
Hin1II CATG 6 cut(s) 173, 331, 449, 454, 518, 537
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HindIII AAGCTT 1 cut(s) 220
HinfI GANTC 3 cut(s) 146, 202, 273
HpaII CCGG 1 cut(s) 571
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 2 cut(s) 260, 364
Hpy188I TCNGA 2 cut(s) 319, 403
Hpy188III TCNNGA 1 cut(s) 299
Hpy8I GTNNAC 2 cut(s) 260, 364
HpyAV CCTTC 2 cut(s) 38, 80
HpyCH4III ACNGT 5 cut(s) 182, 323, 477, 547, 589
HpyCH4V TGCA 8 cut(s) 108, 152, 185, 248, 347, 431, 551, 596
HpyF10VI GCNNNNNNNGC 2 cut(s) 437, 446
HpyF3I CTNAG 3 cut(s) 27, 63, 402
Hsp92II CATG 6 cut(s) 173, 331, 449, 454, 518, 537
Kzo9I GATC 3 cut(s) 117, 295, 565
LpnPI CCDG 7 cut(s) 33, 118, 148, 184, 211, 276, 584
Lsp1109I GCAGC 3 cut(s) 253, 319, 563
LweI GCATC 2 cut(s) 194, 334
MaeI CTAG 3 cut(s) 233, 555, 611
MalI GATC 3 cut(s) 119, 297, 567
MboI GATC 3 cut(s) 117, 295, 565
MboII GAAGA 1 cut(s) 466
MfeI CAATTG 3 cut(s) 189, 348, 432
MhlI GDGCHC 1 cut(s) 130
MluCI AATT 6 cut(s) 12, 97, 189, 348, 375, 432
MlyI GAGTC 2 cut(s) 140, 211
MnlI CCTC 5 cut(s) 66, 69, 245, 248, 417
MseI TTAA 4 cut(s) 36, 140, 384, 495
MspI CCGG 1 cut(s) 571
MspR9I CCNGG 1 cut(s) 199
MunI CAATTG 3 cut(s) 189, 348, 432
MvaI CCWGG 1 cut(s) 199
MwoI GCNNNNNNNGC 2 cut(s) 437, 446
NcoI CCATGG 1 cut(s) 533
NdeII GATC 3 cut(s) 117, 295, 565
NlaIII CATG 6 cut(s) 173, 331, 449, 454, 518, 537
NlaIV GGNNCC 1 cut(s) 439
PaqCI CACCTGC 1 cut(s) 113
PfeI GAWTC 1 cut(s) 273
PkrI GCNGC 4 cut(s) 92, 268, 334, 553
PleI GAGTC 2 cut(s) 140, 210
PpsI GAGTC 2 cut(s) 140, 210
PsiI TTATAA 1 cut(s) 504
Psp6I CCWGG 1 cut(s) 197
PspGI CCWGG 1 cut(s) 197
PspN4I GGNNCC 1 cut(s) 439
PspPI GGNCC 1 cut(s) 53
PstNI CAGNNNCTG 1 cut(s) 149
RsaI GTAC 3 cut(s) 244, 261, 559
RsaNI GTAC 3 cut(s) 243, 260, 558
SaqAI TTAA 4 cut(s) 36, 140, 384, 495
SatI GCNGC 4 cut(s) 91, 267, 333, 552
Sau3AI GATC 3 cut(s) 117, 295, 565
Sau96I GGNCC 1 cut(s) 53
SchI GAGTC 2 cut(s) 140, 211
ScrFI CCNGG 1 cut(s) 199
SduI GDGCHC 1 cut(s) 130
SfaNI GCATC 2 cut(s) 194, 334
SinI GGWCC 1 cut(s) 53
SpeI ACTAGT 1 cut(s) 610
Sse9I AATT 6 cut(s) 12, 97, 189, 348, 375, 432
SsiI CCGC 1 cut(s) 90
SspMI CTAG 3 cut(s) 233, 555, 611
StyD4I CCNGG 1 cut(s) 197
StyI CCWWGG 1 cut(s) 533
TaaI ACNGT 5 cut(s) 182, 323, 477, 547, 589
TaqI TCGA 1 cut(s) 130
TasI AATT 6 cut(s) 12, 97, 189, 348, 375, 432
TauI GCSGC 1 cut(s) 93
TfiI GAWTC 1 cut(s) 273
Tru1I TTAA 4 cut(s) 36, 140, 384, 495
Tru9I TTAA 4 cut(s) 36, 140, 384, 495
TscAI CASTG 3 cut(s) 13, 185, 592
TseI GCWGC 3 cut(s) 266, 332, 551
TspDTI ATGAA 4 cut(s) 57, 372, 434, 467
TspGWI ACGGA 2 cut(s) 243, 615
TspRI CASTG 3 cut(s) 13, 185, 592
VpaK11BI GGWCC 1 cut(s) 53
XapI RAATTY 2 cut(s) 12, 97
XspI CTAG 3 cut(s) 233, 555, 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.