pycom07g09910

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0016808
Physical Location & Seq
Reverse (-)
9515196 .. 9517794
2599 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g09910.1

Sequence Viewer

Length: 1422 bp
ATGAGTAAAATTTACAATTTTTTTTTAAAAAAAACCTGTTATTGCACAAAAGCACTTTGCGCGACGTTGAAATGTATACCTGCGTCTTTCAAAAAATTTCCCAAATTTTCCAAACCAAAAGCCTTCCGCTCTTTATCTATCTCGCACTCACCGACTATCCTCCTCAACCAATTCCACTCGAGTTCCAGAATCACTCTCAGACTCTCAGTCCCTCGACTCCCTCTCCCTCGACACCCTTCCCTCGATTCTCCAACTCTCAATCGTCCTCCTCCTCATAGTGCTCACCAGCCATCACCGCCGTCGACCAACACAACGGCACCACCCATCATCCTTCTCGATGACATCCCAGAGAACCCAGGCCATCACCCCATCACCCCCGTCGTCATCCCTCTAAAATTTAGTTCTGAATCTGCTTTATATGCTGCAACTGAAAGTGATATCATTGTTGGTGTGATGGACACTCGAATTTGGCCTGAAGCCGAGAGCTTTAAGGATGAAGGTTTTGGTCCTCCTCCTAAGAAGTGGAAAGGTGTTTGTGAAGGCGGCAAAAATTTCACCTGCAACAAGTATGTCTCTCAATATTTACATGGTCATGGAACTCACTGTGCATCAATTGTAGCCAGGAGTACTGTAAAGGGTGCAAGCTTTTACGGACTAGCAAATGGTATTGCAAGAGGAGGTGTACCTGCTGCGAGAATCGCAGCATATAAAGTCTGTGATCTTGGTTTCGGGTGTAGTTCAGATGGTATCTTGGCTGCTTTTGACGATGCAATTGCTGATGTAGTTGACATCATTTCAATTTCACTTAATGGATTTTTTCGTTCTGAGCCTCATTTTTATATTGACCGGATTGCAATTGGTGCCCTTCGTGCCATCAAGAAAGGGATACTAACTTCACAGTCTGCCAGAACCATATTTAATAGTTATAAGGTTGGTTCTGTGTCAAGTGTAGCACCATGGATATTCACAGTTGCAGCTAGTACCACATATCGCCGGATTAACAGCACTGTTCTTGCAAATGGAAAGAAACTAGTCGGGCACTCTACGAACACGTTCACATTAAATGGAACAAATTTTCCTCTCATATATGGAAAAGATGCTTCAAGAACAAATTGCTCTCAAATGCTAGCTAGTATATGTTGGGAAGGTTGCCTGGACCCTGATTTAGTTAAGGGAAAGATATTTGTATGTGAGAAGAACACTGGAATTCTTGGGGCTTACAGATCTGGGGCAGTTGGGATAATTTTTGTTAATTTTATGAACAAAACTTTTTCTCAAGTTCTCCCATTTCCTGGGCTAAATGTGGTCAAGTCCTACATGAACTCCACCACGTATAGATTCATTTCAATACTGTTAAGTCATAAAAGATGTTGCTGCGCCTCTTGTTTCTATCACGTCTGCGACTGGACCGAATTTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

474

Amino Acids

51.64

Weight (kDa)

9.39

Isoelectric Point (pI)

35.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 145 - 339 2.9e-15 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 927
AarI CACCTGC 1 cut(s) 566
Acc36I ACCTGC 3 cut(s) 88, 566, 694
AccB1I GGYRCC 2 cut(s) 316, 860
AccB7I CCANNNNNTGG 1 cut(s) 1292
AccBSI CCGCTC 1 cut(s) 129
AccI GTMKAC 2 cut(s) 76, 302
AccII CGCG 1 cut(s) 62
AciI CCGC 3 cut(s) 127, 296, 543
AcsI RAATTY 9 cut(s) 9, 95, 104, 395, 465, 550, 1072, 1206, 1412
AcuI CTGAAG 1 cut(s) 495
AfaI GTAC 3 cut(s) 628, 684, 982
AfiI CCNNNNNNNGG 1 cut(s) 1292
AflIII ACRYGT 1 cut(s) 1050
AgsI TTSAA 5 cut(s) 70, 91, 798, 1104, 1347
AhdI GACNNNNNGTC 1 cut(s) 206
AhlI ACTAGT 1 cut(s) 1030
AjiI CACGTC 1 cut(s) 1396
AjnI CCWGG 4 cut(s) 355, 620, 1152, 1291
AloI GAACNNNNNNTCC 2 cut(s) 1060, 1092
AluBI AGCT 4 cut(s) 486, 645, 977, 1130
AluI AGCT 4 cut(s) 486, 645, 977, 1130
Alw21I GWGCWC 1 cut(s) 283
Alw26I GTCTC 1 cut(s) 577
Ama87I CYCGRG 1 cut(s) 178
AoxI GGCC 2 cut(s) 358, 470
ApeKI GCWGC 6 cut(s) 422, 689, 701, 755, 974, 1374
ApoI RAATTY 9 cut(s) 9, 95, 104, 395, 465, 550, 1072, 1206, 1412
Asp700I GAANNNNTTC 1 cut(s) 1052
AspLEI GCGC 2 cut(s) 62, 1379
AspS9I GGNCC 3 cut(s) 506, 1156, 1407
AsuHPI GGTGA 6 cut(s) 141, 275, 285, 356, 364, 547
AsuNHI GCTAGC 1 cut(s) 1126
AvaI CYCGRG 1 cut(s) 178
AvaII GGWCC 3 cut(s) 506, 1156, 1407
BaeGI GKGCMC 2 cut(s) 865, 1041
BanI GGYRCC 2 cut(s) 316, 860
Bbv12I GWGCWC 1 cut(s) 283
BbvI GCAGC 6 cut(s) 409, 676, 713, 742, 986, 1361
BccI CCATC 7 cut(s) 298, 332, 369, 377, 448, 737, 881
BceAI ACGGC 2 cut(s) 283, 330
BcgI CGANNNNNNTGC 2 cut(s) 755, 789
BciT130I CCWGG 4 cut(s) 357, 622, 1154, 1293
BciVI GTATCC 1 cut(s) 879
BcoDI GTCTC 1 cut(s) 577
BcuI ACTAGT 1 cut(s) 1030
BfaI CTAG 5 cut(s) 656, 978, 1031, 1127, 1131
BfuAI ACCTGC 3 cut(s) 88, 566, 694
BfuI GTATCC 1 cut(s) 879
BglII AGATCT 1 cut(s) 1223
BisI GCNGC 7 cut(s) 423, 544, 690, 702, 756, 975, 1375
BlsI GCNGC 7 cut(s) 424, 545, 691, 703, 757, 976, 1376
BmcAI AGTACT 1 cut(s) 628
Bme1390I CCNGG 4 cut(s) 357, 622, 1154, 1293
Bme18I GGWCC 3 cut(s) 506, 1156, 1407
BmeRI GACNNNNNGTC 1 cut(s) 206
BmeT110I CYCGRG 1 cut(s) 178
BmgBI CACGTC 1 cut(s) 1396
BmgT120I GGNCC 3 cut(s) 506, 1156, 1407
BmiI GGNNCC 3 cut(s) 318, 862, 1158
BmrFI CCNGG 4 cut(s) 357, 622, 1154, 1293
BmsI GCATC 3 cut(s) 617, 757, 1087
BmtI GCTAGC 1 cut(s) 1130
BpuEI CTTGAG 1 cut(s) 1260
BsaAI YACGTR 1 cut(s) 1332
BsaJI CCNNGG 3 cut(s) 355, 956, 1292
BsaWI WCCGGW 1 cut(s) 846
BsaXI ACNNNNNCTCC 4 cut(s) 207, 237, 1307, 1337
Bsc4I CCNNNNNNNGG 1 cut(s) 1292
Bse1I ACTGG 2 cut(s) 1207, 1409
BseBI CCWGG 4 cut(s) 357, 622, 1154, 1293
BseDI CCNNGG 3 cut(s) 355, 956, 1292
BseGI GGATG 4 cut(s) 327, 342, 384, 499
BseLI CCNNNNNNNGG 1 cut(s) 1292
BseMII CTCAG 3 cut(s) 211, 219, 816
BseNI ACTGG 2 cut(s) 1207, 1409
BseRI GAGGAG 5 cut(s) 152, 258, 261, 501, 690
BseSI GKGCMC 2 cut(s) 865, 1041
BseXI GCAGC 6 cut(s) 409, 676, 713, 742, 986, 1361
Bsh1236I CGCG 1 cut(s) 62
BshFI GGCC 2 cut(s) 360, 472
BshNI GGYRCC 2 cut(s) 316, 860
BsiHKAI GWGCWC 1 cut(s) 283
BsiHKCI CYCGRG 1 cut(s) 178
BsiSI CCGG 2 cut(s) 847, 994
BslFI GGGAC 1 cut(s) 194
BslI CCNNNNNNNGG 1 cut(s) 1292
BsmAI GTCTC 1 cut(s) 577
BsmFI GGGAC 1 cut(s) 194
BsnI GGCC 2 cut(s) 360, 472
BsoBI CYCGRG 1 cut(s) 178
Bsp1286I GDGCHC 3 cut(s) 283, 865, 1041
Bsp143I GATC 2 cut(s) 718, 1223
Bsp19I CCATGG 1 cut(s) 956
BspACI CCGC 3 cut(s) 127, 296, 543
BspANI GGCC 2 cut(s) 360, 472
BspCNI CTCAG 3 cut(s) 210, 218, 817
BspFNI CGCG 1 cut(s) 62
BspLI GGNNCC 3 cut(s) 318, 862, 1158
BspMI ACCTGC 3 cut(s) 88, 566, 694
BspOI GCTAGC 1 cut(s) 1130
BspT107I GGYRCC 2 cut(s) 316, 860
BsrBI CCGCTC 1 cut(s) 129
BsrI ACTGG 2 cut(s) 1207, 1409
BssECI CCNNGG 3 cut(s) 355, 956, 1292
BssMI GATC 2 cut(s) 718, 1223
BssNAI GTATAC 1 cut(s) 77
BssT1I CCWWGG 1 cut(s) 956
Bst1107I GTATAC 1 cut(s) 77
Bst2UI CCWGG 4 cut(s) 357, 622, 1154, 1293
Bst4CI ACNGT 6 cut(s) 605, 631, 900, 970, 1009, 1353
BstAPI GCANNNNNTGC 1 cut(s) 860
BstBAI YACGTR 1 cut(s) 1332
BstC8I GCNNGC 2 cut(s) 643, 1128
BstDEI CTNAG 4 cut(s) 197, 205, 516, 825
BstDSI CCRYGG 1 cut(s) 956
BstF5I GGATG 4 cut(s) 327, 342, 384, 499
BstFNI CGCG 1 cut(s) 62
BstHHI GCGC 2 cut(s) 62, 1379
BstKTI GATC 2 cut(s) 721, 1226
BstMAI GTCTC 1 cut(s) 577
BstMBI GATC 2 cut(s) 718, 1223
BstMWI GCNNNNNNNGC 6 cut(s) 59, 295, 419, 698, 860, 869
BstNI CCWGG 4 cut(s) 357, 622, 1154, 1293
BstSCI CCNGG 4 cut(s) 355, 620, 1152, 1291
BstSLI GKGCMC 2 cut(s) 865, 1041
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 6 cut(s) 409, 676, 713, 742, 986, 1361
BstX2I RGATCY 1 cut(s) 1223
BstYI RGATCY 1 cut(s) 1223
BstZ17I GTATAC 1 cut(s) 77
BsuI GTATCC 1 cut(s) 879
BsuRI GGCC 2 cut(s) 360, 472
BtgI CCRYGG 1 cut(s) 956
BtrI CACGTC 1 cut(s) 1396
BtsCI GGATG 4 cut(s) 327, 342, 384, 499
BtsIMutI CAGTG 3 cut(s) 601, 1005, 1200
BveI ACCTGC 3 cut(s) 88, 566, 694
Cac8I GCNNGC 2 cut(s) 643, 1128
CfoI GCGC 2 cut(s) 62, 1379
Cfr13I GGNCC 3 cut(s) 506, 1156, 1407
CseI GACGC 1 cut(s) 72
Csp6I GTAC 3 cut(s) 627, 683, 981
CviAII CATG 4 cut(s) 587, 593, 957, 1318
CviQI GTAC 3 cut(s) 627, 683, 981
DdeI CTNAG 4 cut(s) 197, 205, 516, 825
DpnI GATC 2 cut(s) 720, 1225
DpnII GATC 2 cut(s) 718, 1223
DraI TTTAAA 1 cut(s) 27
DriI GACNNNNNGTC 1 cut(s) 206
Eam1105I GACNNNNNGTC 1 cut(s) 206
Eco130I CCWWGG 1 cut(s) 956
Eco32I GATATC 1 cut(s) 439
Eco47I GGWCC 3 cut(s) 506, 1156, 1407
Eco57I CTGAAG 1 cut(s) 495
Eco88I CYCGRG 1 cut(s) 178
EcoRI GAATTC 1 cut(s) 1206
EcoRII CCWGG 4 cut(s) 355, 620, 1152, 1291
EcoRV GATATC 1 cut(s) 439
EcoT14I CCWWGG 1 cut(s) 956
ErhI CCWWGG 1 cut(s) 956
FaeI CATG 4 cut(s) 590, 596, 960, 1321
FaqI GGGAC 1 cut(s) 194
FatI CATG 4 cut(s) 586, 592, 956, 1317
FblI GTMKAC 2 cut(s) 76, 302
Fnu4HI GCNGC 7 cut(s) 423, 544, 690, 702, 756, 975, 1375
FokI GGATG 4 cut(s) 314, 329, 371, 506
Fsp4HI GCNGC 7 cut(s) 423, 544, 690, 702, 756, 975, 1375
FspBI CTAG 5 cut(s) 656, 978, 1031, 1127, 1131
GlaI GCGC 2 cut(s) 61, 1378
GluI GCNGC 7 cut(s) 423, 544, 690, 702, 756, 975, 1375
HaeIII GGCC 2 cut(s) 360, 472
HapII CCGG 2 cut(s) 847, 994
HgaI GACGC 1 cut(s) 72
HhaI GCGC 2 cut(s) 62, 1379
Hin1II CATG 4 cut(s) 590, 596, 960, 1321
Hin6I GCGC 2 cut(s) 60, 1377
HinP1I GCGC 2 cut(s) 60, 1377
HincII GTYRAC 2 cut(s) 303, 787
HindII GTYRAC 2 cut(s) 303, 787
HindIII AAGCTT 1 cut(s) 643
HinfI GANTC 7 cut(s) 189, 201, 216, 245, 407, 696, 1338
HpaII CCGG 2 cut(s) 847, 994
HphI GGTGA 6 cut(s) 141, 275, 285, 356, 364, 547
Hpy166II GTNNAC 5 cut(s) 77, 303, 683, 787, 1056
Hpy188I TCNGA 4 cut(s) 200, 406, 742, 826
Hpy188III TCNNGA 4 cut(s) 186, 335, 877, 1104
Hpy8I GTNNAC 5 cut(s) 77, 303, 683, 787, 1056
Hpy99I CGWCG 3 cut(s) 67, 304, 383
HpyAV CCTTC 7 cut(s) 133, 246, 341, 491, 533, 875, 1139
HpyCH4III ACNGT 6 cut(s) 605, 631, 900, 970, 1009, 1353
HpyCH4IV ACGT 4 cut(s) 65, 1052, 1331, 1395
HpyF10VI GCNNNNNNNGC 6 cut(s) 59, 295, 419, 698, 860, 869
HpyF3I CTNAG 4 cut(s) 197, 205, 516, 825
HpySE526I ACGT 4 cut(s) 65, 1052, 1331, 1395
Hsp92II CATG 4 cut(s) 590, 596, 960, 1321
HspAI GCGC 2 cut(s) 60, 1377
Kzo9I GATC 2 cut(s) 718, 1223
Lsp1109I GCAGC 6 cut(s) 409, 676, 713, 742, 986, 1361
LweI GCATC 3 cut(s) 617, 757, 1087
MaeI CTAG 5 cut(s) 656, 978, 1031, 1127, 1131
MaeII ACGT 4 cut(s) 65, 1052, 1331, 1395
MalI GATC 2 cut(s) 720, 1225
MbiI CCGCTC 1 cut(s) 129
MboI GATC 2 cut(s) 718, 1223
MboII GAAGA 1 cut(s) 1207
MfeI CAATTG 3 cut(s) 612, 771, 855
MflI RGATCY 1 cut(s) 1223
MhlI GDGCHC 3 cut(s) 283, 865, 1041
MlyI GAGTC 2 cut(s) 195, 210
MmeI TCCRAC 1 cut(s) 275
MroXI GAANNNNTTC 1 cut(s) 1052
MseI TTAA 9 cut(s) 26, 489, 807, 918, 999, 1061, 1170, 1251, 1355
MslI CAYNNNNRTG 1 cut(s) 591
MspI CCGG 2 cut(s) 847, 994
MspR9I CCNGG 4 cut(s) 357, 622, 1154, 1293
MunI CAATTG 3 cut(s) 612, 771, 855
MvaI CCWGG 4 cut(s) 357, 622, 1154, 1293
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 6 cut(s) 59, 295, 419, 698, 860, 869
NcoI CCATGG 1 cut(s) 956
NdeII GATC 2 cut(s) 718, 1223
NheI GCTAGC 1 cut(s) 1126
NlaIII CATG 4 cut(s) 590, 596, 960, 1321
NlaIV GGNNCC 3 cut(s) 318, 862, 1158
NmeAIII GCCGAG 1 cut(s) 505
PaeR7I CTCGAG 1 cut(s) 178
PaqCI CACCTGC 1 cut(s) 566
PcsI WCGNNNNNNNCGW 1 cut(s) 149
PdmI GAANNNNTTC 1 cut(s) 1052
PfeI GAWTC 5 cut(s) 189, 245, 407, 696, 1338
PflMI CCANNNNNTGG 1 cut(s) 1292
PkrI GCNGC 7 cut(s) 424, 545, 691, 703, 757, 976, 1376
PleI GAGTC 2 cut(s) 195, 210
PpsI GAGTC 2 cut(s) 195, 210
Ppu21I YACGTR 1 cut(s) 1332
PsiI TTATAA 1 cut(s) 927
Psp6I CCWGG 4 cut(s) 355, 620, 1152, 1291
PspGI CCWGG 4 cut(s) 355, 620, 1152, 1291
PspN4I GGNNCC 3 cut(s) 318, 862, 1158
PspPI GGNCC 3 cut(s) 506, 1156, 1407
PspXI VCTCGAGB 1 cut(s) 178
PsuI RGATCY 1 cut(s) 1223
RsaI GTAC 3 cut(s) 628, 684, 982
RsaNI GTAC 3 cut(s) 627, 683, 981
RseI CAYNNNNRTG 1 cut(s) 591
SalI GTCGAC 1 cut(s) 301
SaqAI TTAA 9 cut(s) 26, 489, 807, 918, 999, 1061, 1170, 1251, 1355
SatI GCNGC 7 cut(s) 423, 544, 690, 702, 756, 975, 1375
Sau3AI GATC 2 cut(s) 718, 1223
Sau96I GGNCC 3 cut(s) 506, 1156, 1407
ScaI AGTACT 1 cut(s) 628
SchI GAGTC 2 cut(s) 195, 210
ScrFI CCNGG 4 cut(s) 357, 622, 1154, 1293
SduI GDGCHC 3 cut(s) 283, 865, 1041
SfaNI GCATC 3 cut(s) 617, 757, 1087
Sfr274I CTCGAG 1 cut(s) 178
SinI GGWCC 3 cut(s) 506, 1156, 1407
SlaI CTCGAG 1 cut(s) 178
SmiMI CAYNNNNRTG 1 cut(s) 591
SmlI CTYRAG 2 cut(s) 178, 1275
SmoI CTYRAG 2 cut(s) 178, 1275
SpeI ACTAGT 1 cut(s) 1030
SsiI CCGC 3 cut(s) 127, 296, 543
SspI AATATT 1 cut(s) 581
SspMI CTAG 5 cut(s) 656, 978, 1031, 1127, 1131
StyD4I CCNGG 4 cut(s) 355, 620, 1152, 1291
StyI CCWWGG 1 cut(s) 956
TaaI ACNGT 6 cut(s) 605, 631, 900, 970, 1009, 1353
TaiI ACGT 4 cut(s) 68, 1055, 1334, 1398
TaqI TCGA 7 cut(s) 179, 214, 229, 243, 302, 336, 463
TatI WGTACW 1 cut(s) 626
TauI GCSGC 1 cut(s) 546
TfiI GAWTC 5 cut(s) 189, 245, 407, 696, 1338
Tru1I TTAA 9 cut(s) 26, 489, 807, 918, 999, 1061, 1170, 1251, 1355
Tru9I TTAA 9 cut(s) 26, 489, 807, 918, 999, 1061, 1170, 1251, 1355
TscAI CASTG 3 cut(s) 608, 1012, 1207
TseI GCWGC 6 cut(s) 422, 689, 701, 755, 974, 1374
TspDTI ATGAA 4 cut(s) 510, 1274, 1330, 1334
TspGWI ACGGA 1 cut(s) 666
TspRI CASTG 3 cut(s) 608, 1012, 1207
Van91I CCANNNNNTGG 1 cut(s) 1292
VpaK11BI GGWCC 3 cut(s) 506, 1156, 1407
XapI RAATTY 9 cut(s) 9, 95, 104, 395, 465, 550, 1072, 1206, 1412
XhoI CTCGAG 1 cut(s) 178
XmiI GTMKAC 2 cut(s) 76, 302
XmnI GAANNNNTTC 1 cut(s) 1052
XspI CTAG 5 cut(s) 656, 978, 1031, 1127, 1131
ZrmI AGTACT 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.