Rroxscaffold_4G00322790

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0016808
Physical Location & Seq
Reverse (-)
53303029 .. 53303726
698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00322790.1

Sequence Viewer

Length: 621 bp
ATGAAGGTTTTGGTCCTCCTCCCAAGAAGTGGAAAGGATGCAAGGGATCAAATAGGCCATGGAACCCACACAGCCTCAACTACAGCAGGGAACGTTGTAAAGGATGTGAACTTTTATGGACTAGCACAAGGTACTGCAAGAGGAAGGGTTCCCTCGCCTAGAATTGCTGCGTATAAAGTCCGTGATGCTTTTGGGTGTTCTACAGAGAATATCTTGGCTGCTTTTGATGATGCTATTGTCGATGGAGTTGACATCATTACAATTTCAATAGGAAGCATGCAAGCATCTTTATTACAGCATGATCCTATTGCTATTGGTGTTTTTCATGCAATGACAAAGGGGATACTTACATCAAATTCTGCAGCAACAAGTGGTCCCGATGATTCAAGTGTTGCAAGTGTAGCACCATGGATACTTGCAGTTCCAACAAGTAGTACTAATCGTAGAATCATTGACAACGTTGTTCTCGAAAATGGGACGACATTGGTTAGGGCTACAGTGAACACTTTCACATTAAATGGGACAAGTTTTCCATTGATATATGGAAAAGATGCTTCAACTCAATGCTCGAGTCCAAAGCCGAGAGTTGTACGAAGGCTGCTTAGACAGTGGTTTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

206

Amino Acids

21.85

Weight (kDa)

9.51

Isoelectric Point (pI)

28.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 12 - 135 2.9e-10 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 29
AclI AACGTT 2 cut(s) 93, 459
AclWI GGATC 2 cut(s) 54, 296
AcsI RAATTY 1 cut(s) 355
AfaI GTAC 3 cut(s) 133, 436, 591
AfiI CCNNNNNNNGG 1 cut(s) 29
AgsI TTSAA 3 cut(s) 267, 387, 558
AlwI GGATC 2 cut(s) 54, 296
Ama87I CYCGRG 1 cut(s) 568
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 4 cut(s) 167, 218, 362, 598
ApoI RAATTY 1 cut(s) 355
Asp700I GAANNNNTTC 1 cut(s) 506
AspS9I GGNCC 2 cut(s) 13, 374
AvaI CYCGRG 1 cut(s) 568
AvaII GGWCC 2 cut(s) 13, 374
BbvI GCAGC 4 cut(s) 154, 205, 374, 585
BccI CCATC 1 cut(s) 236
BciVI GTATCC 2 cut(s) 336, 405
BfaI CTAG 2 cut(s) 122, 159
BfmI CTRYAG 4 cut(s) 81, 201, 360, 495
BfuI GTATCC 2 cut(s) 336, 405
BisI GCNGC 4 cut(s) 168, 219, 363, 599
BlsI GCNGC 4 cut(s) 169, 220, 364, 600
BmcAI AGTACT 1 cut(s) 436
Bme18I GGWCC 2 cut(s) 13, 374
BmeT110I CYCGRG 1 cut(s) 568
BmgT120I GGNCC 2 cut(s) 13, 374
BmiI GGNNCC 3 cut(s) 64, 150, 376
BmsI GCATC 5 cut(s) 28, 175, 220, 293, 541
BsaJI CCNNGG 2 cut(s) 58, 407
Bsc4I CCNNNNNNNGG 1 cut(s) 29
Bse3DI GCAATG 1 cut(s) 336
BseDI CCNNGG 2 cut(s) 58, 407
BseGI GGATG 2 cut(s) 43, 109
BseLI CCNNNNNNNGG 1 cut(s) 29
BseMI GCAATG 1 cut(s) 336
BseRI GAGGAG 1 cut(s) 8
BseXI GCAGC 4 cut(s) 154, 205, 374, 585
BshFI GGCC 1 cut(s) 57
BsiHKCI CYCGRG 1 cut(s) 568
BslFI GGGAC 3 cut(s) 360, 490, 535
BslI CCNNNNNNNGG 1 cut(s) 29
BsmFI GGGAC 3 cut(s) 360, 490, 535
BsnI GGCC 1 cut(s) 57
BsoBI CYCGRG 1 cut(s) 568
Bsp143I GATC 2 cut(s) 46, 301
Bsp19I CCATGG 2 cut(s) 58, 407
BspANI GGCC 1 cut(s) 57
BspLI GGNNCC 3 cut(s) 64, 150, 376
BspMAI CTGCAG 1 cut(s) 364
BspPI GGATC 2 cut(s) 54, 296
BsrDI GCAATG 1 cut(s) 336
BssECI CCNNGG 2 cut(s) 58, 407
BssMI GATC 2 cut(s) 46, 301
BssT1I CCWWGG 2 cut(s) 58, 407
Bst4CI ACNGT 2 cut(s) 499, 609
BstC8I GCNNGC 2 cut(s) 278, 282
BstDEI CTNAG 1 cut(s) 602
BstDSI CCRYGG 2 cut(s) 58, 407
BstF5I GGATG 2 cut(s) 43, 109
BstKTI GATC 2 cut(s) 49, 304
BstMBI GATC 2 cut(s) 46, 301
BstMWI GCNNNNNNNGC 1 cut(s) 401
BstNSI RCATGY 1 cut(s) 280
BstSFI CTRYAG 4 cut(s) 81, 201, 360, 495
BstV1I GCAGC 4 cut(s) 154, 205, 374, 585
BsuI GTATCC 2 cut(s) 336, 405
BsuRI GGCC 1 cut(s) 57
BtgI CCRYGG 2 cut(s) 58, 407
BtsCI GGATG 2 cut(s) 43, 109
BtsIMutI CAGTG 2 cut(s) 504, 614
Cac8I GCNNGC 2 cut(s) 278, 282
Cfr13I GGNCC 2 cut(s) 13, 374
Csp6I GTAC 3 cut(s) 132, 435, 590
CviAII CATG 5 cut(s) 59, 277, 299, 326, 408
CviJI RGCY 6 cut(s) 57, 74, 218, 494, 580, 598
CviKI_1 RGCY 6 cut(s) 57, 74, 218, 494, 580, 598
CviQI GTAC 3 cut(s) 132, 435, 590
DdeI CTNAG 1 cut(s) 602
DpnI GATC 2 cut(s) 48, 303
DpnII GATC 2 cut(s) 46, 301
Eco130I CCWWGG 2 cut(s) 58, 407
Eco47I GGWCC 2 cut(s) 13, 374
Eco88I CYCGRG 1 cut(s) 568
EcoT14I CCWWGG 2 cut(s) 58, 407
ErhI CCWWGG 2 cut(s) 58, 407
FaeI CATG 5 cut(s) 62, 280, 302, 329, 411
FaiI YATR 9 cut(s) 60, 117, 174, 278, 300, 327, 409, 541, 543
FaqI GGGAC 3 cut(s) 360, 490, 535
FatI CATG 5 cut(s) 58, 276, 298, 325, 407
Fnu4HI GCNGC 4 cut(s) 168, 219, 363, 599
FokI GGATG 2 cut(s) 50, 116
Fsp4HI GCNGC 4 cut(s) 168, 219, 363, 599
FspBI CTAG 2 cut(s) 122, 159
GluI GCNGC 4 cut(s) 168, 219, 363, 599
HaeIII GGCC 1 cut(s) 57
Hin1II CATG 5 cut(s) 62, 280, 302, 329, 411
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HinfI GANTC 3 cut(s) 383, 447, 571
Hpy166II GTNNAC 3 cut(s) 109, 250, 502
Hpy188III TCNNGA 2 cut(s) 377, 467
Hpy8I GTNNAC 3 cut(s) 109, 250, 502
HpyAV CCTTC 2 cut(s) 138, 588
HpyCH4III ACNGT 2 cut(s) 499, 609
HpyCH4IV ACGT 2 cut(s) 93, 459
HpyCH4V TGCA 7 cut(s) 41, 137, 280, 329, 362, 395, 419
HpyF10VI GCNNNNNNNGC 1 cut(s) 401
HpyF3I CTNAG 1 cut(s) 602
HpySE526I ACGT 2 cut(s) 93, 459
Hsp92II CATG 5 cut(s) 62, 280, 302, 329, 411
Kzo9I GATC 2 cut(s) 46, 301
LpnPI CCDG 1 cut(s) 72
Lsp1109I GCAGC 4 cut(s) 154, 205, 374, 585
LweI GCATC 5 cut(s) 28, 175, 220, 293, 541
MaeI CTAG 2 cut(s) 122, 159
MaeII ACGT 2 cut(s) 93, 459
MalI GATC 2 cut(s) 48, 303
MboI GATC 2 cut(s) 46, 301
MluCI AATT 3 cut(s) 162, 261, 355
MlyI GAGTC 1 cut(s) 580
MmeI TCCRAC 1 cut(s) 449
MnlI CCTC 5 cut(s) 26, 29, 85, 134, 163
MroXI GAANNNNTTC 1 cut(s) 506
MseI TTAA 2 cut(s) 515, 619
MwoI GCNNNNNNNGC 1 cut(s) 401
NcoI CCATGG 2 cut(s) 58, 407
NdeII GATC 2 cut(s) 46, 301
NlaIII CATG 5 cut(s) 62, 280, 302, 329, 411
NlaIV GGNNCC 3 cut(s) 64, 150, 376
NmeAIII GCCGAG 1 cut(s) 606
NspI RCATGY 1 cut(s) 280
PaeI GCATGC 1 cut(s) 280
PaeR7I CTCGAG 1 cut(s) 568
PcsI WCGNNNNNNNCGW 1 cut(s) 465
PdmI GAANNNNTTC 1 cut(s) 506
PfeI GAWTC 2 cut(s) 383, 447
PflMI CCANNNNNTGG 1 cut(s) 29
PkrI GCNGC 4 cut(s) 169, 220, 364, 600
PleI GAGTC 1 cut(s) 579
PpsI GAGTC 1 cut(s) 579
Psp1406I AACGTT 2 cut(s) 93, 459
PspN4I GGNNCC 3 cut(s) 64, 150, 376
PspPI GGNCC 2 cut(s) 13, 374
PspXI VCTCGAGB 1 cut(s) 568
PstI CTGCAG 1 cut(s) 364
RsaI GTAC 3 cut(s) 133, 436, 591
RsaNI GTAC 3 cut(s) 132, 435, 590
SaqAI TTAA 2 cut(s) 515, 619
SatI GCNGC 4 cut(s) 168, 219, 363, 599
Sau3AI GATC 2 cut(s) 46, 301
Sau96I GGNCC 2 cut(s) 13, 374
ScaI AGTACT 1 cut(s) 436
SchI GAGTC 1 cut(s) 580
SetI ASST 4 cut(s) 9, 96, 133, 462
SfaNI GCATC 5 cut(s) 28, 175, 220, 293, 541
SfcI CTRYAG 4 cut(s) 81, 201, 360, 495
Sfr274I CTCGAG 1 cut(s) 568
SinI GGWCC 2 cut(s) 13, 374
SlaI CTCGAG 1 cut(s) 568
SmlI CTYRAG 1 cut(s) 568
SmoI CTYRAG 1 cut(s) 568
SphI GCATGC 1 cut(s) 280
Sse9I AATT 3 cut(s) 162, 261, 355
SspMI CTAG 2 cut(s) 122, 159
StyI CCWWGG 2 cut(s) 58, 407
TaaI ACNGT 2 cut(s) 499, 609
TaiI ACGT 2 cut(s) 96, 462
TaqI TCGA 3 cut(s) 240, 468, 569
TasI AATT 3 cut(s) 162, 261, 355
TatI WGTACW 1 cut(s) 434
TfiI GAWTC 2 cut(s) 383, 447
Tru1I TTAA 2 cut(s) 515, 619
Tru9I TTAA 2 cut(s) 515, 619
TscAI CASTG 2 cut(s) 504, 614
TseI GCWGC 4 cut(s) 167, 218, 362, 598
TspDTI ATGAA 2 cut(s) 17, 314
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 2 cut(s) 504, 614
Van91I CCANNNNNTGG 1 cut(s) 29
VpaK11BI GGWCC 2 cut(s) 13, 374
XapI RAATTY 1 cut(s) 355
XceI RCATGY 1 cut(s) 280
XhoI CTCGAG 1 cut(s) 568
XmnI GAANNNNTTC 1 cut(s) 506
XspI CTAG 2 cut(s) 122, 159
ZrmI AGTACT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.