Rh1AG082700

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0016808
Physical Location & Seq
Forward (+)
14176710 .. 14178249
1540 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG082700.1

Sequence Viewer

Length: 837 bp
ATGGCTAAGCATGGAGCTATCCTATTTTCTTATGTTTTCCCCAGTCTTATACTTACCTTGAACTTCTTCCTGTGCAAAGCCATCGATGAAAACAGAAAGGATGCAAGGGATCAAATAGGCCATGGAACCCACACAGCCTCAACTGCAGCAGGAAACGTTGTAAAGGATGTGAGCTTTTACGGACTGGCACAAGGTACTGCAAGAGGAGGGGTTCCCTCAGCCAGAATTGCTGCGTATAAAGTCTGCGATGCTTTTGGGTGTGCTACAGAGAATATCTTGGCTGCTTTTGACGATGCTATTGCCGATGGAGTTGATATCATTACAATTTCAATAGGAAGTGTGCAAGCAGCTTTATTACAGCATGATCCTATTGCTATTGGTGCTTTTCATGCAATGACAAAGGGGATACTTACATCAAATTCTGCAGGCAACACTGGTCCTGATCATTCAAGTGTTGTAAGTGTAGCACCATGGATACTTGCAGTTGCAGCAAGTAGTACTGATCGTAGAATCATTGACAACGTTGTTCTCGAAAATGGGACGACATTGGTTGGGGCTACAGTGAACACTTTCACATTAAATGGGACAAGTTTTCCATTGATATATGGAAAAGATGCTTCAAGTCAATGCTCCGAGTCCAAAGCTGAGAGTTGTACAGAAGGCTGCTTAGACAGTGATTTAGTTAAGGGAAAGGTTGTGTTATGTGATGAGCCTTTCGGAGTTTATGAAGCCAATAGTTCTGGAGCAGTAGGCTCGATTCTGAGATTTGATTGGGATGATGTTTCTCCAATTGTACCCTTTCCTGCAACAGGAGTCTTGAAGACACAAGGCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

278

Amino Acids

28.9

Weight (kDa)

4.92

Isoelectric Point (pI)

30.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 30 - 222 2.6e-17 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 2 cut(s) 156, 522
AclWI GGATC 2 cut(s) 117, 359
AcsI RAATTY 1 cut(s) 418
AfaI GTAC 4 cut(s) 196, 499, 655, 795
AfiI CCNNNNNNNGG 1 cut(s) 809
AgsI TTSAA 5 cut(s) 61, 330, 450, 621, 820
AluBI AGCT 4 cut(s) 17, 174, 350, 644
AluI AGCT 4 cut(s) 17, 174, 350, 644
AlwI GGATC 2 cut(s) 117, 359
AoxI GGCC 1 cut(s) 118
ApeKI GCWGC 6 cut(s) 146, 230, 281, 347, 488, 663
ApoI RAATTY 1 cut(s) 418
Asp700I GAANNNNTTC 2 cut(s) 65, 569
AspS9I GGNCC 1 cut(s) 437
AvaII GGWCC 1 cut(s) 437
BbsI GAAGAC 1 cut(s) 827
BbvCI CCTCAGC 1 cut(s) 217
BbvI GCAGC 6 cut(s) 158, 217, 268, 359, 500, 650
BccI CCATC 2 cut(s) 89, 299
BcgI CGANNNNNNTGC 6 cut(s) 64, 98, 281, 315, 735, 769
BciVI GTATCC 2 cut(s) 399, 468
BclI TGATCA 1 cut(s) 442
BfmI CTRYAG 4 cut(s) 144, 264, 423, 558
BfuI GTATCC 2 cut(s) 399, 468
BisI GCNGC 6 cut(s) 147, 231, 282, 348, 489, 664
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 6 cut(s) 148, 232, 283, 349, 490, 665
BmcAI AGTACT 1 cut(s) 499
Bme18I GGWCC 1 cut(s) 437
BmgT120I GGNCC 1 cut(s) 437
BmiI GGNNCC 2 cut(s) 127, 213
BmrI ACTGGG 1 cut(s) 36
BmsI GCATC 4 cut(s) 91, 238, 283, 604
BmuI ACTGGG 1 cut(s) 36
BpiI GAAGAC 1 cut(s) 827
BpmI CTGGAG 1 cut(s) 762
Bpu10I CCTNAGC 1 cut(s) 217
Bpu1102I GCTNAGC 1 cut(s) 6
Bsa29I ATCGAT 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 121, 470
Bsc4I CCNNNNNNNGG 1 cut(s) 809
Bse1I ACTGG 3 cut(s) 42, 189, 439
Bse3DI GCAATG 1 cut(s) 399
BseCI ATCGAT 1 cut(s) 84
BseDI CCNNGG 2 cut(s) 121, 470
BseGI GGATG 3 cut(s) 106, 172, 781
BseLI CCNNNNNNNGG 1 cut(s) 809
BseMI GCAATG 1 cut(s) 399
BseMII CTCAG 3 cut(s) 231, 636, 752
BseNI ACTGG 3 cut(s) 42, 189, 439
BseRI GAGGAG 1 cut(s) 219
BseXI GCAGC 6 cut(s) 158, 217, 268, 359, 500, 650
BshFI GGCC 1 cut(s) 120
BshVI ATCGAT 1 cut(s) 84
BslFI GGGAC 2 cut(s) 553, 598
BslI CCNNNNNNNGG 1 cut(s) 809
BsmFI GGGAC 2 cut(s) 553, 598
BsnI GGCC 1 cut(s) 120
Bsp1407I TGTACA 1 cut(s) 653
Bsp143I GATC 4 cut(s) 109, 364, 442, 502
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 2 cut(s) 121, 470
BspANI GGCC 1 cut(s) 120
BspCNI CTCAG 3 cut(s) 230, 637, 753
BspDI ATCGAT 1 cut(s) 84
BspLI GGNNCC 2 cut(s) 127, 213
BspMAI CTGCAG 2 cut(s) 148, 427
BspPI GGATC 2 cut(s) 117, 359
BsrDI GCAATG 1 cut(s) 399
BsrGI TGTACA 1 cut(s) 653
BsrI ACTGG 3 cut(s) 42, 189, 439
BssECI CCNNGG 2 cut(s) 121, 470
BssMI GATC 4 cut(s) 109, 364, 442, 502
BssT1I CCWWGG 2 cut(s) 121, 470
Bst4CI ACNGT 2 cut(s) 562, 674
BstAUI TGTACA 1 cut(s) 653
BstC8I GCNNGC 2 cut(s) 345, 427
BstDEI CTNAG 5 cut(s) 6, 217, 645, 667, 761
BstDSI CCRYGG 2 cut(s) 121, 470
BstENI CCTNNNNNAGG 1 cut(s) 807
BstF5I GGATG 3 cut(s) 106, 172, 781
BstKTI GATC 4 cut(s) 112, 367, 445, 505
BstMBI GATC 4 cut(s) 109, 364, 442, 502
BstMWI GCNNNNNNNGC 5 cut(s) 143, 227, 380, 389, 488
BstNSI RCATGY 1 cut(s) 835
BstSFI CTRYAG 4 cut(s) 144, 264, 423, 558
BstV1I GCAGC 6 cut(s) 158, 217, 268, 359, 500, 650
BstV2I GAAGAC 1 cut(s) 827
Bsu15I ATCGAT 1 cut(s) 84
BsuI GTATCC 2 cut(s) 399, 468
BsuRI GGCC 1 cut(s) 120
BsuTUI ATCGAT 1 cut(s) 84
BtgI CCRYGG 2 cut(s) 121, 470
BtgZI GCGATG 1 cut(s) 261
BtsCI GGATG 3 cut(s) 106, 172, 781
BtsIMutI CAGTG 3 cut(s) 432, 567, 679
Cac8I GCNNGC 2 cut(s) 345, 427
Cfr13I GGNCC 1 cut(s) 437
ClaI ATCGAT 1 cut(s) 84
Csp6I GTAC 4 cut(s) 195, 498, 654, 794
CviAII CATG 6 cut(s) 11, 122, 362, 389, 471, 832
CviQI GTAC 4 cut(s) 195, 498, 654, 794
DdeI CTNAG 5 cut(s) 6, 217, 645, 667, 761
DpnI GATC 4 cut(s) 111, 366, 444, 504
DpnII GATC 4 cut(s) 109, 364, 442, 502
Eco130I CCWWGG 2 cut(s) 121, 470
Eco32I GATATC 1 cut(s) 316
Eco47I GGWCC 1 cut(s) 437
EcoNI CCTNNNNNAGG 1 cut(s) 807
EcoRV GATATC 1 cut(s) 316
EcoT14I CCWWGG 2 cut(s) 121, 470
ErhI CCWWGG 2 cut(s) 121, 470
FaeI CATG 6 cut(s) 14, 125, 365, 392, 474, 835
FaqI GGGAC 2 cut(s) 553, 598
FatI CATG 6 cut(s) 10, 121, 361, 388, 470, 831
FbaI TGATCA 1 cut(s) 442
Fnu4HI GCNGC 6 cut(s) 147, 231, 282, 348, 489, 664
FokI GGATG 3 cut(s) 113, 179, 788
Fsp4HI GCNGC 6 cut(s) 147, 231, 282, 348, 489, 664
GluI GCNGC 6 cut(s) 147, 231, 282, 348, 489, 664
GsuI CTGGAG 1 cut(s) 762
HaeIII GGCC 1 cut(s) 120
Hin1II CATG 6 cut(s) 14, 125, 365, 392, 474, 835
HinfI GANTC 4 cut(s) 510, 635, 757, 813
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 3 cut(s) 634, 719, 762
Hpy188III TCNNGA 4 cut(s) 440, 530, 741, 817
Hpy8I GTNNAC 1 cut(s) 565
HpyAV CCTTC 1 cut(s) 653
HpyCH4III ACNGT 2 cut(s) 562, 674
HpyCH4IV ACGT 2 cut(s) 156, 522
HpyF10VI GCNNNNNNNGC 5 cut(s) 143, 227, 380, 389, 488
HpyF3I CTNAG 5 cut(s) 6, 217, 645, 667, 761
HpySE526I ACGT 2 cut(s) 156, 522
Hsp92II CATG 6 cut(s) 14, 125, 365, 392, 474, 835
Ksp22I TGATCA 1 cut(s) 442
Kzo9I GATC 4 cut(s) 109, 364, 442, 502
LmnI GCTCC 3 cut(s) 14, 635, 743
Lsp1109I GCAGC 6 cut(s) 158, 217, 268, 359, 500, 650
LweI GCATC 4 cut(s) 91, 238, 283, 604
MaeII ACGT 2 cut(s) 156, 522
MalI GATC 4 cut(s) 111, 366, 444, 504
MboI GATC 4 cut(s) 109, 364, 442, 502
MboII GAAGA 2 cut(s) 58, 832
MfeI CAATTG 1 cut(s) 789
MluCI AATT 4 cut(s) 225, 324, 418, 789
MlyI GAGTC 2 cut(s) 644, 822
MnlI CCTC 4 cut(s) 148, 197, 200, 226
MroXI GAANNNNTTC 2 cut(s) 65, 569
MseI TTAA 2 cut(s) 578, 684
MslI CAYNNNNRTG 1 cut(s) 450
MunI CAATTG 1 cut(s) 789
MwoI GCNNNNNNNGC 5 cut(s) 143, 227, 380, 389, 488
NcoI CCATGG 2 cut(s) 121, 470
NdeII GATC 4 cut(s) 109, 364, 442, 502
NlaIII CATG 6 cut(s) 14, 125, 365, 392, 474, 835
NlaIV GGNNCC 2 cut(s) 127, 213
NspI RCATGY 1 cut(s) 835
PcsI WCGNNNNNNNCGW 1 cut(s) 528
PdmI GAANNNNTTC 2 cut(s) 65, 569
PfeI GAWTC 2 cut(s) 510, 757
PkrI GCNGC 6 cut(s) 148, 232, 283, 349, 490, 665
PleI GAGTC 2 cut(s) 643, 821
PpsI GAGTC 2 cut(s) 643, 821
Psp1406I AACGTT 2 cut(s) 156, 522
PspN4I GGNNCC 2 cut(s) 127, 213
PspPI GGNCC 1 cut(s) 437
PstI CTGCAG 2 cut(s) 148, 427
RsaI GTAC 4 cut(s) 196, 499, 655, 795
RsaNI GTAC 4 cut(s) 195, 498, 654, 794
RseI CAYNNNNRTG 1 cut(s) 450
SaqAI TTAA 2 cut(s) 578, 684
SatI GCNGC 6 cut(s) 147, 231, 282, 348, 489, 664
Sau3AI GATC 4 cut(s) 109, 364, 442, 502
Sau96I GGNCC 1 cut(s) 437
ScaI AGTACT 1 cut(s) 499
SchI GAGTC 2 cut(s) 644, 822
SetI ASST 9 cut(s) 19, 59, 159, 176, 196, 352, 525, 646, 696
SfaNI GCATC 4 cut(s) 91, 238, 283, 604
SfcI CTRYAG 4 cut(s) 144, 264, 423, 558
SinI GGWCC 1 cut(s) 437
SmiMI CAYNNNNRTG 1 cut(s) 450
Sse9I AATT 4 cut(s) 225, 324, 418, 789
StyI CCWWGG 2 cut(s) 121, 470
TaaI ACNGT 2 cut(s) 562, 674
TaiI ACGT 2 cut(s) 159, 525
TaqI TCGA 3 cut(s) 84, 531, 755
TasI AATT 4 cut(s) 225, 324, 418, 789
TatI WGTACW 2 cut(s) 497, 653
TfiI GAWTC 2 cut(s) 510, 757
Tru1I TTAA 2 cut(s) 578, 684
Tru9I TTAA 2 cut(s) 578, 684
TscAI CASTG 3 cut(s) 439, 567, 679
TseI GCWGC 6 cut(s) 146, 230, 281, 347, 488, 663
TspDTI ATGAA 3 cut(s) 102, 377, 741
TspGWI ACGGA 1 cut(s) 195
TspRI CASTG 3 cut(s) 439, 567, 679
VpaK11BI GGWCC 1 cut(s) 437
XagI CCTNNNNNAGG 1 cut(s) 807
XapI RAATTY 1 cut(s) 418
XceI RCATGY 1 cut(s) 835
XmnI GAANNNNTTC 2 cut(s) 65, 569
ZrmI AGTACT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.