pycom01g09810

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0016808
Physical Location & Seq
Reverse (-)
10605866 .. 10607438
1573 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g09810.5

Sequence Viewer

Length: 690 bp
ATGAAATGTATACCTGCGTCGCGCATTTGTGATAATTTTGGCGGGGCAATAGTAAAAGATTTTCAAAAAATTTCCCAAATTTCCCAAACCAAAAGCCTTCCGCTCTTTATCTATCTCCCGACTATCCTCCTCAACCAATTCCACTCGAGTTCCAGAATCACTCTCAGACTCTCAGTCCCTCGACTCCCTCTCCCTCAACACCCTTCCCTCAACTCTCCAACTCTCAATCGTCCTCCTCCTCATAGTGCTCACCAGCCATCATCGCCGTCGACCAACACAACGGCACCACCCATCATCCTTCTCGATGACATCCTAGAGAACCCAGGCCATCACCCCATCACCCCCGTCGTCATCCCTCTAAAATTTAGTTCTGAATCTGCTTTATATGCTGCAACTGAAAGTGATATCATTGTTGGTGTGATGGACACTCGAATTTGGCCTGAAGCCGAGAGCTTTAAAGATGAAGGTTTTGGTCCTCCTCCTAAGAAGTGGAAACGTGTTTGTGAAGGCGGCAAAAATTTCACCTGCAACAAAAAGATTGTTGGTGCTCGATATTACTTAACAGACTCTGCAAGGGATTTCCAGGGTCATGGAACTCACTGTGCATCAATTGTAGCCAGGAGTACTGTAAAGGATGCAAGCTTTTACGGACTAGCAAATGGTATTGCAAGAGGAGGTTTTCGTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

230

Amino Acids

25.07

Weight (kDa)

9.38

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 533
Acc36I ACCTGC 2 cut(s) 22, 533
AccB1I GGYRCC 1 cut(s) 283
AccBSI CCGCTC 1 cut(s) 103
AccI GTMKAC 2 cut(s) 10, 269
AccII CGCG 1 cut(s) 22
AciI CCGC 3 cut(s) 42, 101, 510
AcsI RAATTY 5 cut(s) 69, 78, 362, 432, 517
AcuI CTGAAG 1 cut(s) 462
AfaI GTAC 1 cut(s) 625
AflIII ACRYGT 1 cut(s) 496
AgsI TTSAA 1 cut(s) 65
AhdI GACNNNNNGTC 1 cut(s) 173
AjnI CCWGG 3 cut(s) 322, 582, 617
AluBI AGCT 2 cut(s) 453, 642
AluI AGCT 2 cut(s) 453, 642
Alw21I GWGCWC 2 cut(s) 250, 550
AlwNI CAGNNNCTG 1 cut(s) 569
Ama87I CYCGRG 1 cut(s) 145
AoxI GGCC 2 cut(s) 325, 437
ApeKI GCWGC 1 cut(s) 389
ApoI RAATTY 5 cut(s) 69, 78, 362, 432, 517
AspLEI GCGC 1 cut(s) 24
AspS9I GGNCC 1 cut(s) 473
AsuHPI GGTGA 4 cut(s) 242, 323, 331, 514
AvaI CYCGRG 1 cut(s) 145
AvaII GGWCC 1 cut(s) 473
BanI GGYRCC 1 cut(s) 283
Bbv12I GWGCWC 2 cut(s) 250, 550
BbvI GCAGC 1 cut(s) 376
BccI CCATC 5 cut(s) 265, 299, 336, 344, 415
BceAI ACGGC 2 cut(s) 250, 297
BciT130I CCWGG 3 cut(s) 324, 584, 619
BfaI CTAG 2 cut(s) 314, 653
BfuAI ACCTGC 2 cut(s) 22, 533
BisI GCNGC 2 cut(s) 390, 511
BlsI GCNGC 2 cut(s) 391, 512
BmcAI AGTACT 1 cut(s) 625
Bme1390I CCNGG 3 cut(s) 324, 584, 619
Bme18I GGWCC 1 cut(s) 473
BmeRI GACNNNNNGTC 1 cut(s) 173
BmeT110I CYCGRG 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 473
BmiI GGNNCC 1 cut(s) 285
BmrFI CCNGG 3 cut(s) 324, 584, 619
BmsI GCATC 2 cut(s) 614, 625
BsaJI CCNNGG 2 cut(s) 322, 583
BsaXI ACNNNNNCTCC 2 cut(s) 174, 204
BseBI CCWGG 3 cut(s) 324, 584, 619
BseDI CCNNGG 2 cut(s) 322, 583
BseGI GGATG 4 cut(s) 294, 309, 351, 640
BseMII CTCAG 2 cut(s) 178, 186
BseRI GAGGAG 5 cut(s) 119, 225, 228, 468, 687
BseXI GCAGC 1 cut(s) 376
Bsh1236I CGCG 1 cut(s) 22
BshFI GGCC 2 cut(s) 327, 439
BshNI GGYRCC 1 cut(s) 283
BsiHKAI GWGCWC 2 cut(s) 250, 550
BsiHKCI CYCGRG 1 cut(s) 145
BslFI GGGAC 1 cut(s) 161
BsmFI GGGAC 1 cut(s) 161
BsnI GGCC 2 cut(s) 327, 439
BsoBI CYCGRG 1 cut(s) 145
Bsp1286I GDGCHC 2 cut(s) 250, 550
BspACI CCGC 3 cut(s) 42, 101, 510
BspANI GGCC 2 cut(s) 327, 439
BspCNI CTCAG 2 cut(s) 177, 185
BspFNI CGCG 1 cut(s) 22
BspLI GGNNCC 1 cut(s) 285
BspMI ACCTGC 2 cut(s) 22, 533
BspT107I GGYRCC 1 cut(s) 283
BsrBI CCGCTC 1 cut(s) 103
BssECI CCNNGG 2 cut(s) 322, 583
BssNAI GTATAC 1 cut(s) 11
Bst1107I GTATAC 1 cut(s) 11
Bst2UI CCWGG 3 cut(s) 324, 584, 619
Bst4CI ACNGT 2 cut(s) 602, 628
BstC8I GCNNGC 1 cut(s) 640
BstDEI CTNAG 3 cut(s) 164, 172, 483
BstF5I GGATG 4 cut(s) 294, 309, 351, 640
BstFNI CGCG 1 cut(s) 22
BstHHI GCGC 1 cut(s) 24
BstMWI GCNNNNNNNGC 2 cut(s) 262, 386
BstNI CCWGG 3 cut(s) 324, 584, 619
BstSCI CCNGG 3 cut(s) 322, 582, 617
BstUI CGCG 1 cut(s) 22
BstV1I GCAGC 1 cut(s) 376
BstXI CCANNNNNNTGG 1 cut(s) 590
BstZ17I GTATAC 1 cut(s) 11
BsuRI GGCC 2 cut(s) 327, 439
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 4 cut(s) 294, 309, 351, 640
BtsIMutI CAGTG 1 cut(s) 598
BveI ACCTGC 2 cut(s) 22, 533
Cac8I GCNNGC 1 cut(s) 640
CaiI CAGNNNCTG 1 cut(s) 569
CfoI GCGC 1 cut(s) 24
Cfr13I GGNCC 1 cut(s) 473
CseI GACGC 1 cut(s) 6
Csp6I GTAC 1 cut(s) 624
CviAII CATG 1 cut(s) 590
CviJI RGCY 8 cut(s) 96, 256, 327, 439, 446, 453, 617, 642
CviKI_1 RGCY 8 cut(s) 96, 256, 327, 439, 446, 453, 617, 642
CviQI GTAC 1 cut(s) 624
DdeI CTNAG 3 cut(s) 164, 172, 483
DraI TTTAAA 1 cut(s) 457
DriI GACNNNNNGTC 1 cut(s) 173
Eam1105I GACNNNNNGTC 1 cut(s) 173
Eco32I GATATC 1 cut(s) 406
Eco47I GGWCC 1 cut(s) 473
Eco57I CTGAAG 1 cut(s) 462
Eco88I CYCGRG 1 cut(s) 145
EcoRII CCWGG 3 cut(s) 322, 582, 617
EcoRV GATATC 1 cut(s) 406
FaeI CATG 1 cut(s) 593
FaiI YATR 5 cut(s) 11, 243, 385, 387, 591
FaqI GGGAC 1 cut(s) 161
FatI CATG 1 cut(s) 589
FauI CCCGC 1 cut(s) 35
FblI GTMKAC 2 cut(s) 10, 269
Fnu4HI GCNGC 2 cut(s) 390, 511
FokI GGATG 4 cut(s) 281, 296, 338, 647
Fsp4HI GCNGC 2 cut(s) 390, 511
FspBI CTAG 2 cut(s) 314, 653
GlaI GCGC 1 cut(s) 23
GluI GCNGC 2 cut(s) 390, 511
HaeIII GGCC 2 cut(s) 327, 439
HgaI GACGC 1 cut(s) 6
HhaI GCGC 1 cut(s) 24
Hin1II CATG 1 cut(s) 593
Hin6I GCGC 1 cut(s) 22
HinP1I GCGC 1 cut(s) 22
HincII GTYRAC 1 cut(s) 270
HindII GTYRAC 1 cut(s) 270
HindIII AAGCTT 1 cut(s) 640
HinfI GANTC 5 cut(s) 156, 168, 183, 374, 566
HphI GGTGA 4 cut(s) 242, 323, 331, 514
Hpy166II GTNNAC 2 cut(s) 11, 270
Hpy188I TCNGA 2 cut(s) 167, 373
Hpy188III TCNNGA 3 cut(s) 118, 153, 302
Hpy8I GTNNAC 2 cut(s) 11, 270
Hpy99I CGWCG 3 cut(s) 22, 271, 350
HpyAV CCTTC 5 cut(s) 107, 213, 308, 458, 500
HpyCH4III ACNGT 2 cut(s) 602, 628
HpyCH4IV ACGT 1 cut(s) 496
HpyCH4V TGCA 6 cut(s) 392, 528, 572, 605, 638, 668
HpyF10VI GCNNNNNNNGC 2 cut(s) 262, 386
HpyF3I CTNAG 3 cut(s) 164, 172, 483
HpySE526I ACGT 1 cut(s) 496
Hsp92II CATG 1 cut(s) 593
HspAI GCGC 1 cut(s) 22
Lsp1109I GCAGC 1 cut(s) 376
LweI GCATC 2 cut(s) 614, 625
MaeI CTAG 2 cut(s) 314, 653
MaeII ACGT 1 cut(s) 496
MbiI CCGCTC 1 cut(s) 103
MfeI CAATTG 1 cut(s) 609
MhlI GDGCHC 2 cut(s) 250, 550
MluCI AATT 8 cut(s) 34, 69, 78, 137, 362, 432, 517, 609
MlyI GAGTC 3 cut(s) 162, 177, 560
MmeI TCCRAC 1 cut(s) 242
MseI TTAA 2 cut(s) 456, 560
MspR9I CCNGG 3 cut(s) 324, 584, 619
MunI CAATTG 1 cut(s) 609
MvaI CCWGG 3 cut(s) 324, 584, 619
MvnI CGCG 1 cut(s) 22
MwoI GCNNNNNNNGC 2 cut(s) 262, 386
NlaIII CATG 1 cut(s) 593
NlaIV GGNNCC 1 cut(s) 285
NmeAIII GCCGAG 1 cut(s) 472
PaeR7I CTCGAG 1 cut(s) 145
PaqCI CACCTGC 1 cut(s) 533
PfeI GAWTC 2 cut(s) 156, 374
PkrI GCNGC 2 cut(s) 391, 512
PleI GAGTC 3 cut(s) 162, 177, 560
PpsI GAGTC 3 cut(s) 162, 177, 560
Psp6I CCWGG 3 cut(s) 322, 582, 617
PspGI CCWGG 3 cut(s) 322, 582, 617
PspN4I GGNNCC 1 cut(s) 285
PspPI GGNCC 1 cut(s) 473
PspXI VCTCGAGB 1 cut(s) 145
PstNI CAGNNNCTG 1 cut(s) 569
RsaI GTAC 1 cut(s) 625
RsaNI GTAC 1 cut(s) 624
SalI GTCGAC 1 cut(s) 268
SaqAI TTAA 2 cut(s) 456, 560
SatI GCNGC 2 cut(s) 390, 511
Sau96I GGNCC 1 cut(s) 473
ScaI AGTACT 1 cut(s) 625
SchI GAGTC 3 cut(s) 162, 177, 560
ScrFI CCNGG 3 cut(s) 324, 584, 619
SduI GDGCHC 2 cut(s) 250, 550
SetI ASST 7 cut(s) 16, 455, 469, 499, 527, 644, 679
SfaNI GCATC 2 cut(s) 614, 625
Sfr274I CTCGAG 1 cut(s) 145
SinI GGWCC 1 cut(s) 473
SlaI CTCGAG 1 cut(s) 145
SmlI CTYRAG 1 cut(s) 145
SmoI CTYRAG 1 cut(s) 145
Sse9I AATT 8 cut(s) 34, 69, 78, 137, 362, 432, 517, 609
SsiI CCGC 3 cut(s) 42, 101, 510
SspMI CTAG 2 cut(s) 314, 653
StyD4I CCNGG 3 cut(s) 322, 582, 617
TaaI ACNGT 2 cut(s) 602, 628
TaiI ACGT 1 cut(s) 499
TaqI TCGA 6 cut(s) 146, 181, 269, 303, 430, 550
TasI AATT 8 cut(s) 34, 69, 78, 137, 362, 432, 517, 609
TatI WGTACW 1 cut(s) 623
TauI GCSGC 1 cut(s) 513
TfiI GAWTC 2 cut(s) 156, 374
Tru1I TTAA 2 cut(s) 456, 560
Tru9I TTAA 2 cut(s) 456, 560
TscAI CASTG 1 cut(s) 605
TseI GCWGC 1 cut(s) 389
TspDTI ATGAA 2 cut(s) 17, 477
TspGWI ACGGA 1 cut(s) 663
TspRI CASTG 1 cut(s) 605
VpaK11BI GGWCC 1 cut(s) 473
XapI RAATTY 5 cut(s) 69, 78, 362, 432, 517
XhoI CTCGAG 1 cut(s) 145
XmiI GTMKAC 2 cut(s) 10, 269
XspI CTAG 2 cut(s) 314, 653
ZrmI AGTACT 1 cut(s) 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.