Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0016808

Physical Location & Seq

Genomic Coordinates Reverse (-)

12250758 .. 12253919

Gene Structure 3162 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1133600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2103 bp

ATGGCTAAGCATGGAGCTTCTCTGTGTTATTATCCTTTCTTCACTCTCTTCTTACTCACAATGATCTTGTTATGCAAGGCCGCTGACGAGGAAAGAAGGGTCCATATTGTGTACATGGGATCGCTTCCTGATGGGAATCCAGCGTACTCACCGTTGTCTCACCACCTTAGTATTCTACAGCGAGTTGTGCGGGGAAGTTTGGCAGAAACTATATTGGTCAGAAGTTATAAAAGGAGTTTCAATGGATTTGCAGCCAACCTCACTGACCAAGAAAGGAAGAAAATTGCGAACATGGAAGAAATAGTCTCTGTCTTTCCGAGCATAAGAATGGAACCTCAAACAACAAGGTCTTGGGACTTTATGGGTTTCGATGAGAAAGTCAGTCGAAATGCCACAACTGAAAGTGATATCATTGTTGGCGTGATGGACACTGGAATTTGGCCTGAAGCTGAGGGCTTTAAAGATGAAGGTTTTGGTCCTCCTCCTAAGAAGTGGAAAGGTGTTTGTGAAGGCGGCAAAAATTTCACCTGCAACAAGTATATCGTTGGTGCTCGATATTACTTAACAGACTCTGCAAGGGATTTCGGGGGTCATGGAACTCACTGTGCATCAATTGTAGCCGGGAGTCCTGTAAAGGATGTAAGCTTCTACAGACTAGCAAACGGTACTGCAGGAGGAGGTGTACCTGCTGCGAGAATCGCAGCATATAAAGTCTGTGATCTTGATTTCGGGTGTAGTTCAGATGGTATCTTGGCTGCTTTTGACGATGCAATTGCCGATGAAGTTGACCTCATTTCAAATTCACTTAATGGATTTTTTCGTTCTGAGCCTCATTTTTATATTGACCCGGTTGCAATTGGTGCCTTTCATGCCATGAAGAAAGGGATACTAACTTCACAGTCTGCCGGAACCATATTTAATAGTGATAAGGTTGGTCCTGTGTCAAGTGTAGCACCATGGATATTCACAGTTGCAGCTAGTACCACAGATCGCCGGATTATTGACAGCACTGTTCTTGCAAACGGAAAGAAACTAGTCGGGCACTCTACGAACACGTTCACATTAAATGGAACAAATTTTCCTCTCGTATATGGAAAAGATGCTTCAAGAACAAATTGCTCTCAAATGCTAGCTAGTATATGTTGGGAAGGTTGCCTGGACCCTGATTTAGTTAAGGGAAAGATAGTTGTATGTGAGAAGAACACTGGAATTCCTGGGACTTACAGATCTGGGGCAGTTGGGATAATTTTTGTTAATTTTATGAACGAAACTTCTTCTCAAGTTCTCCCATTTCCTGGAGTGAATTTAATCACAGAAGAGCTAAATGTGGTCAAGTCCTACATGAACTCCACCAGGGTCCCTCAAGCGACGATATTACAAAGTGAAGTCATAAAAGATGTTGCTGCACCTCTTGTTTCTATCACGTCTGCAACTGGACCGAATTTAGTTGTACCCGATATTATTAAGCCAGATATAAGTGCCCCAGGCGTAGAAATTTTGGCTGCATATTCAACTTTTGCTACTGCCAGCGAATATCCAGAAGACAATAGGCATGTAAAGTACAATATACTAAGTGGAACCTCCGTATCTTGCCCCCACGTTGCCGGGGCAGCTGCATATGTTAAATCATTCCATCCTGATTGGTCTCTGGCTGCCATCAAATCATCTCTTATGACTACTGTCTATGATGAAGGAAAAGTTAGGCTCATAACAGGAGAAAATATCACATGCCCTAAAGATCCTGCTAAATGGTCTCCACGGGATCATAATTACCCTTTAATGGGAGCTAAAGTTGATCCGATGAAACCTTTCACGGTTCAATTTCATAGAACAGTTAAAAATGTTGGCCTTGCGAACTCCACATATAGGGCTAAAATCTTTTCAAACTCCAAACTTGATATCAAAGTGGTGCCTGAAGTTCTTTCGTTCAAGTCCTTGAACGAGCAGATGGATTTTGACGTGACTGTTGGTGGAAGCGATTTGCAAGATCGATCACAAGTATCTGGGTCACTGGTCTGGTCTGATGGTACTCATAGCGTTAGAAGTCCAATCCTGGTTTATGCATATGTTCCAATAAGCTCCAGAACATGGTTACATGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

701

Amino Acids

76.13

Weight (kDa)

6.77

Isoelectric Point (pI)

36.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	34 - 111	3.4e-14	Peptidase inhibitor I9
Peptidase_S8	PF00082	135 - 561	1.7e-37	Subtilase family
fn3_6	PF17766	588 - 686	1.1e-24	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016808)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g37161
malus_domestica	MD09G1128100.v1.1 MD11G1133600.v1.1
pyrus_communis	pycom01g09800 pycom01g09810 pycom03g06640 pycom07g09910
rosa_roxburghii	Rroxscaffold_4G00322790
rosa_samantha	Rh1AG055600 Rh1AG082700 Rh1CG056200 Rh1CG080700 Rh1DG060100 Rh1DG087300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	228
AarI	CACCTGC	1 cut(s)	536
Acc36I	ACCTGC	2 cut(s)	536, 694
AccB1I	GGYRCC	2 cut(s)	860, 1909
AccB7I	CCANNNNNTGG	2 cut(s)	1295, 2088
AciI	CCGC	3 cut(s)	81, 190, 513
AclWI	GGATC	4 cut(s)	127, 1733, 1770, 1790
AcsI	RAATTY	8 cut(s)	435, 520, 799, 1075, 1209, 1303, 1441, 1494
AcuI	CTGAAG	2 cut(s)	465, 1935
AfaI	GTAC	8 cut(s)	113, 146, 667, 684, 982, 1452, 1562, 2029
AfiI	CCNNNNNNNGG	5 cut(s)	1295, 1780, 1781, 1866, 2088
AflIII	ACRYGT	1 cut(s)	1053
AgsI	TTSAA	8 cut(s)	241, 798, 1107, 1512, 1820, 1884, 1930, 1939
AhlI	ACTAGT	1 cut(s)	1033
AjiI	CACGTC	2 cut(s)	1425, 1960
AjnI	CCWGG	6 cut(s)	1155, 1213, 1294, 1352, 1483, 2052
AloI	GAACNNNNNNTCC	2 cut(s)	1063, 1095
AluBI	AGCT	9 cut(s)	17, 449, 645, 977, 1133, 1321, 1613, 1787, 2079
AluI	AGCT	9 cut(s)	17, 449, 645, 977, 1133, 1321, 1613, 1787, 2079
Alw21I	GWGCWC	1 cut(s)	553
Alw26I	GTCTC	4 cut(s)	162, 310, 1650, 1758
AlwI	GGATC	4 cut(s)	127, 1733, 1770, 1790
AlwNI	CAGNNNCTG	1 cut(s)	572
AoxI	GGCC	3 cut(s)	78, 440, 1846
ApoI	RAATTY	8 cut(s)	435, 520, 799, 1075, 1209, 1303, 1441, 1494
Asp700I	GAANNNNTTC	2 cut(s)	1055, 1808
AspS9I	GGNCC	6 cut(s)	100, 476, 935, 1159, 1357, 1436
AsuC2I	CCSGG	3 cut(s)	622, 848, 1606
AsuHPI	GGTGA	3 cut(s)	141, 152, 517
AsuNHI	GCTAGC	1 cut(s)	1129
AvaII	GGWCC	6 cut(s)	100, 476, 935, 1159, 1357, 1436
BaeGI	GKGCMC	2 cut(s)	1044, 1483
BaeI	ACNNNNGTAYC	2 cut(s)	1569, 1602
BanI	GGYRCC	2 cut(s)	860, 1909
BbsI	GAAGAC	1 cut(s)	1548
Bbv12I	GWGCWC	1 cut(s)	553
BbvCI	CCTCAGC	1 cut(s)	450
BccI	CCATC	7 cut(s)	125, 418, 737, 1641, 1664, 1942, 2018
BcgI	CGANNNNNNTGC	2 cut(s)	755, 789
BciT130I	CCWGG	6 cut(s)	1157, 1215, 1296, 1354, 1485, 2054
BciVI	GTATCC	1 cut(s)	879
BcnI	CCSGG	3 cut(s)	622, 848, 1606
BcoDI	GTCTC	4 cut(s)	162, 310, 1650, 1758
BcuI	ACTAGT	1 cut(s)	1033
BfaI	CTAG	5 cut(s)	656, 978, 1034, 1130, 1134
BfmI	CTRYAG	3 cut(s)	176, 649, 669
BfuAI	ACCTGC	2 cut(s)	536, 694
BfuI	GTATCC	1 cut(s)	879
BglII	AGATCT	1 cut(s)	1226
BlpI	GCTNAGC	1 cut(s)	6
Bme1390I	CCNGG	9 cut(s)	622, 848, 1157, 1215, 1296, 1354, 1485, 1606, 2054
Bme18I	GGWCC	6 cut(s)	100, 476, 935, 1159, 1357, 1436
BmgBI	CACGTC	2 cut(s)	1425, 1960
BmgT120I	GGNCC	6 cut(s)	100, 476, 935, 1159, 1357, 1436
BmiI	GGNNCC	9 cut(s)	101, 333, 862, 910, 1161, 1358, 1359, 1579, 1911
BmrFI	CCNGG	9 cut(s)	622, 848, 1157, 1215, 1296, 1354, 1485, 1606, 2054
BmsI	GCATC	3 cut(s)	617, 757, 1090
BmtI	GCTAGC	1 cut(s)	1133
BoxI	GACNNNNGTC	1 cut(s)	1679
BpiI	GAAGAC	1 cut(s)	1548
BpmI	CTGGAG	2 cut(s)	1317, 2065
Bpu10I	CCTNAGC	1 cut(s)	450
Bpu1102I	GCTNAGC	1 cut(s)	6
BpuEI	CTTGAG	2 cut(s)	1263, 1347
BpuMI	CCSGG	3 cut(s)	622, 848, 1606
Bsa29I	ATCGAT	1 cut(s)	1990
BsaBI	GATNNNNATC	1 cut(s)	135
BsaI	GGTCTC	2 cut(s)	1650, 1758
BsaJI	CCNNGG	6 cut(s)	956, 1214, 1353, 1483, 1605, 1757
BsaXI	ACNNNNNCTCC	6 cut(s)	666, 696, 1331, 1361, 1776, 1806
Bsc4I	CCNNNNNNNGG	5 cut(s)	1295, 1780, 1781, 1866, 2088
Bse1I	ACTGG	4 cut(s)	436, 1210, 1438, 2016
Bse8I	GATNNNNATC	1 cut(s)	135
BseBI	CCWGG	6 cut(s)	1157, 1215, 1296, 1354, 1485, 2054
BseCI	ATCGAT	1 cut(s)	1990
BseDI	CCNNGG	6 cut(s)	956, 1214, 1353, 1483, 1605, 1757
BseGI	GGATG	2 cut(s)	643, 1633
BseJI	GATNNNNATC	1 cut(s)	135
BseLI	CCNNNNNNNGG	5 cut(s)	1295, 1780, 1781, 1866, 2088
BseMII	CTCAG	2 cut(s)	441, 816
BseNI	ACTGG	4 cut(s)	436, 1210, 1438, 2016
BseRI	GAGGAG	2 cut(s)	471, 690
BseSI	GKGCMC	2 cut(s)	1044, 1483
BsgI	GTGCAG	1 cut(s)	1389
BshFI	GGCC	3 cut(s)	80, 442, 1848
BshNI	GGYRCC	2 cut(s)	860, 1909
BshVI	ATCGAT	1 cut(s)	1990
BsiHKAI	GWGCWC	1 cut(s)	553
BsiSI	CCGG	5 cut(s)	621, 848, 906, 994, 1605
BslFI	GGGAC	3 cut(s)	368, 1231, 1343
BslI	CCNNNNNNNGG	5 cut(s)	1295, 1780, 1781, 1866, 2088
BsmAI	GTCTC	4 cut(s)	162, 310, 1650, 1758
BsmFI	GGGAC	3 cut(s)	368, 1231, 1343
BsnI	GGCC	3 cut(s)	80, 442, 1848
Bso31I	GGTCTC	2 cut(s)	1650, 1758
Bsp1286I	GDGCHC	3 cut(s)	553, 1044, 1483
Bsp1407I	TGTACA	1 cut(s)	111
Bsp1720I	GCTNAGC	1 cut(s)	6
Bsp19I	CCATGG	1 cut(s)	956
BspACI	CCGC	3 cut(s)	81, 190, 513
BspANI	GGCC	3 cut(s)	80, 442, 1848
BspCNI	CTCAG	2 cut(s)	442, 817
BspDI	ATCGAT	1 cut(s)	1990
BspLI	GGNNCC	9 cut(s)	101, 333, 862, 910, 1161, 1358, 1359, 1579, 1911
BspMAI	CTGCAG	1 cut(s)	673
BspMI	ACCTGC	2 cut(s)	536, 694
BspOI	GCTAGC	1 cut(s)	1133
BspPI	GGATC	4 cut(s)	127, 1733, 1770, 1790
BspQI	GCTCTTC	1 cut(s)	1311
BspT107I	GGYRCC	2 cut(s)	860, 1909
BspTNI	GGTCTC	2 cut(s)	1650, 1758
BsrGI	TGTACA	1 cut(s)	111
BsrI	ACTGG	4 cut(s)	436, 1210, 1438, 2016
BssECI	CCNNGG	6 cut(s)	956, 1214, 1353, 1483, 1605, 1757
BssT1I	CCWWGG	1 cut(s)	956
Bst2UI	CCWGG	6 cut(s)	1157, 1215, 1296, 1354, 1485, 2054
Bst6I	CTCTTC	2 cut(s)	53, 1311
BstAPI	GCANNNNNTGC	1 cut(s)	860
BstAUI	TGTACA	1 cut(s)	111
BstC8I	GCNNGC	2 cut(s)	1131, 1528
BstDEI	CTNAG	6 cut(s)	6, 167, 450, 486, 825, 1571
BstDSI	CCRYGG	2 cut(s)	956, 1757
BstF5I	GGATG	2 cut(s)	643, 1633
BstMAI	GTCTC	4 cut(s)	162, 310, 1650, 1758
BstMWI	GCNNNNNNNGC	5 cut(s)	187, 698, 860, 869, 1610
BstNI	CCWGG	6 cut(s)	1157, 1215, 1296, 1354, 1485, 2054
BstNSI	RCATGY	2 cut(s)	1556, 1731
BstPAI	GACNNNNGTC	1 cut(s)	1679
BstSCI	CCNGG	9 cut(s)	620, 846, 1155, 1213, 1294, 1352, 1483, 1604, 2052
BstSFI	CTRYAG	3 cut(s)	176, 649, 669
BstSLI	GKGCMC	2 cut(s)	1044, 1483
BstV2I	GAAGAC	1 cut(s)	1548
BstX2I	RGATCY	2 cut(s)	1226, 1738
BstYI	RGATCY	2 cut(s)	1226, 1738
Bsu15I	ATCGAT	1 cut(s)	1990
BsuI	GTATCC	1 cut(s)	879
BsuRI	GGCC	3 cut(s)	80, 442, 1848
BsuTUI	ATCGAT	1 cut(s)	1990
BtgI	CCRYGG	2 cut(s)	956, 1757
BtrI	CACGTC	2 cut(s)	1425, 1960
BtsCI	GGATG	2 cut(s)	643, 1633
BtsIMutI	CAGTG	6 cut(s)	261, 429, 601, 1008, 1203, 2009
BveI	ACCTGC	2 cut(s)	536, 694
Cac8I	GCNNGC	2 cut(s)	1131, 1528
CaiI	CAGNNNCTG	1 cut(s)	572
Cfr13I	GGNCC	6 cut(s)	100, 476, 935, 1159, 1357, 1436
ClaI	ATCGAT	1 cut(s)	1990
Csp6I	GTAC	8 cut(s)	112, 145, 666, 683, 981, 1451, 1561, 2028
CviQI	GTAC	8 cut(s)	112, 145, 666, 683, 981, 1451, 1561, 2028
DdeI	CTNAG	6 cut(s)	6, 167, 450, 486, 825, 1571
DraI	TTTAAA	1 cut(s)	460
Eam1104I	CTCTTC	2 cut(s)	53, 1311
EarI	CTCTTC	2 cut(s)	53, 1311
Eco130I	CCWWGG	1 cut(s)	956
Eco31I	GGTCTC	2 cut(s)	1650, 1758
Eco32I	GATATC	2 cut(s)	409, 1900
Eco47I	GGWCC	6 cut(s)	100, 476, 935, 1159, 1357, 1436
Eco57I	CTGAAG	2 cut(s)	465, 1935
EcoO109I	RGGNCCY	1 cut(s)	1357
EcoRI	GAATTC	1 cut(s)	1209
EcoRII	CCWGG	6 cut(s)	1155, 1213, 1294, 1352, 1483, 2052
EcoRV	GATATC	2 cut(s)	409, 1900
EcoT14I	CCWWGG	1 cut(s)	956
EcoT22I	ATGCAT	1 cut(s)	2065
ErhI	CCWWGG	1 cut(s)	956
FaqI	GGGAC	3 cut(s)	368, 1231, 1343
FauI	CCCGC	1 cut(s)	183
FauNDI	CATATG	2 cut(s)	1618, 2065
FokI	GGATG	2 cut(s)	650, 1620
FspBI	CTAG	5 cut(s)	656, 978, 1034, 1130, 1134
GsuI	CTGGAG	2 cut(s)	1317, 2065
HaeIII	GGCC	3 cut(s)	80, 442, 1848
HapII	CCGG	5 cut(s)	621, 848, 906, 994, 1605
HincII	GTYRAC	1 cut(s)	787
HindII	GTYRAC	1 cut(s)	787
HindIII	AAGCTT	1 cut(s)	643
HinfI	GANTC	4 cut(s)	136, 569, 625, 696
HpaII	CCGG	5 cut(s)	621, 848, 906, 994, 1605
HphI	GGTGA	3 cut(s)	141, 152, 517
Hpy166II	GTNNAC	4 cut(s)	112, 683, 787, 1059
Hpy188I	TCNGA	6 cut(s)	221, 318, 742, 826, 1800, 2023
Hpy188III	TCNNGA	6 cut(s)	128, 722, 1107, 1538, 1637, 2082
Hpy8I	GTNNAC	4 cut(s)	112, 683, 787, 1059
Hpy99I	CGWCG	1 cut(s)	1372
HpyAV	CCTTC	5 cut(s)	90, 461, 503, 1142, 1685
HpyCH4IV	ACGT	4 cut(s)	1055, 1424, 1599, 1959
HpyF10VI	GCNNNNNNNGC	5 cut(s)	187, 698, 860, 869, 1610
HpyF3I	CTNAG	6 cut(s)	6, 167, 450, 486, 825, 1571
HpySE526I	ACGT	4 cut(s)	1055, 1424, 1599, 1959
KflI	GGGWCCC	1 cut(s)	1357
LguI	GCTCTTC	1 cut(s)	1311
LmnI	GCTCC	3 cut(s)	14, 1784, 2084
LweI	GCATC	3 cut(s)	617, 757, 1090
MaeI	CTAG	5 cut(s)	656, 978, 1034, 1130, 1134
MaeII	ACGT	4 cut(s)	1055, 1424, 1599, 1959
MaeIII	GTNAC	3 cut(s)	1960, 2007, 2091
MboII	GAAGA	9 cut(s)	31, 40, 289, 308, 889, 1210, 1266, 1328, 1553
MfeI	CAATTG	3 cut(s)	612, 771, 855
MflI	RGATCY	2 cut(s)	1226, 1738
MhlI	GDGCHC	3 cut(s)	553, 1044, 1483
MlyI	GAGTC	2 cut(s)	563, 634
Mph1103I	ATGCAT	1 cut(s)	2065
MroXI	GAANNNNTTC	2 cut(s)	1055, 1808
MslI	CAYNNNNRTG	1 cut(s)	326
MspA1I	CMGCKG	2 cut(s)	83, 1613
MspI	CCGG	5 cut(s)	621, 848, 906, 994, 1605
MspR9I	CCNGG	9 cut(s)	622, 848, 1157, 1215, 1296, 1354, 1485, 1606, 2054
MunI	CAATTG	3 cut(s)	612, 771, 855
MvaI	CCWGG	6 cut(s)	1157, 1215, 1296, 1354, 1485, 2054
MwoI	GCNNNNNNNGC	5 cut(s)	187, 698, 860, 869, 1610
NciI	CCSGG	3 cut(s)	622, 848, 1606
NcoI	CCATGG	1 cut(s)	956
NdeI	CATATG	2 cut(s)	1618, 2065
NheI	GCTAGC	1 cut(s)	1129
NlaIV	GGNNCC	9 cut(s)	101, 333, 862, 910, 1161, 1358, 1359, 1579, 1911
NmuCI	GTSAC	2 cut(s)	1960, 2007
NsiI	ATGCAT	1 cut(s)	2065
NspI	RCATGY	2 cut(s)	1556, 1731
PaqCI	CACCTGC	1 cut(s)	536
PciSI	GCTCTTC	1 cut(s)	1311
PdmI	GAANNNNTTC	2 cut(s)	1055, 1808
PfeI	GAWTC	2 cut(s)	136, 696
PflMI	CCANNNNNTGG	2 cut(s)	1295, 2088
PfoI	TCCNGGA	1 cut(s)	1294
PleI	GAGTC	2 cut(s)	563, 633
PpsI	GAGTC	2 cut(s)	563, 633
PpuMI	RGGWCCY	1 cut(s)	1357
PshAI	GACNNNNGTC	1 cut(s)	1679
PsiI	TTATAA	1 cut(s)	228
Psp5II	RGGWCCY	1 cut(s)	1357
Psp6I	CCWGG	6 cut(s)	1155, 1213, 1294, 1352, 1483, 2052
PspGI	CCWGG	6 cut(s)	1155, 1213, 1294, 1352, 1483, 2052
PspN4I	GGNNCC	9 cut(s)	101, 333, 862, 910, 1161, 1358, 1359, 1579, 1911
PspPI	GGNCC	6 cut(s)	100, 476, 935, 1159, 1357, 1436
PspPPI	RGGWCCY	1 cut(s)	1357
PstI	CTGCAG	1 cut(s)	673
PstNI	CAGNNNCTG	1 cut(s)	572
PsuI	RGATCY	2 cut(s)	1226, 1738
PvuII	CAGCTG	1 cut(s)	1613
RsaI	GTAC	8 cut(s)	113, 146, 667, 684, 982, 1452, 1562, 2029
RsaNI	GTAC	8 cut(s)	112, 145, 666, 683, 981, 1451, 1561, 2028
RseI	CAYNNNNRTG	1 cut(s)	326
SapI	GCTCTTC	1 cut(s)	1311
Sau96I	GGNCC	6 cut(s)	100, 476, 935, 1159, 1357, 1436
SchI	GAGTC	2 cut(s)	563, 634
ScrFI	CCNGG	9 cut(s)	622, 848, 1157, 1215, 1296, 1354, 1485, 1606, 2054
SduI	GDGCHC	3 cut(s)	553, 1044, 1483
SfaNI	GCATC	3 cut(s)	617, 757, 1090
SfcI	CTRYAG	3 cut(s)	176, 649, 669
SinI	GGWCC	6 cut(s)	100, 476, 935, 1159, 1357, 1436
SmiMI	CAYNNNNRTG	1 cut(s)	326
SmlI	CTYRAG	2 cut(s)	1278, 1362
SmoI	CTYRAG	2 cut(s)	1278, 1362
SpeI	ACTAGT	1 cut(s)	1033
SsiI	CCGC	3 cut(s)	81, 190, 513
SspMI	CTAG	5 cut(s)	656, 978, 1034, 1130, 1134
StyD4I	CCNGG	9 cut(s)	620, 846, 1155, 1213, 1294, 1352, 1483, 1604, 2052
StyI	CCWWGG	1 cut(s)	956
TaiI	ACGT	4 cut(s)	1058, 1427, 1602, 1962
TaqI	TCGA	4 cut(s)	369, 385, 553, 1990
TaqII	GACCGA	1 cut(s)	1453
TatI	WGTACW	2 cut(s)	111, 1560
TauI	GCSGC	2 cut(s)	83, 516
TfiI	GAWTC	2 cut(s)	136, 696
TscAI	CASTG	6 cut(s)	268, 436, 608, 1015, 1210, 2016
TseFI	GTSAC	2 cut(s)	1960, 2007
Tsp45I	GTSAC	2 cut(s)	1960, 2007
TspDTI	ATGAA	9 cut(s)	480, 795, 857, 890, 1277, 1358, 1704, 1814, 1817
TspGWI	ACGGA	2 cut(s)	1038, 1573
TspRI	CASTG	6 cut(s)	268, 436, 608, 1015, 1210, 2016
Van91I	CCANNNNNTGG	2 cut(s)	1295, 2088
VpaK11BI	GGWCC	6 cut(s)	100, 476, 935, 1159, 1357, 1436
XapI	RAATTY	8 cut(s)	435, 520, 799, 1075, 1209, 1303, 1441, 1494
XceI	RCATGY	2 cut(s)	1556, 1731
XmnI	GAANNNNTTC	2 cut(s)	1055, 1808
XspI	CTAG	5 cut(s)	656, 978, 1034, 1130, 1134
Zsp2I	ATGCAT	1 cut(s)	2065

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.