FvH4_3g42650

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007750
Physical Location & Seq
Forward (+)
35626112 .. 35627515
1404 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g42650.t1

Sequence Viewer

Length: 1404 bp
ATGAACATGATTCTGAATTTGTATGCGTTATGTGGGGAAGTTAGGGATGCACGGAAGATGTTCGATAAAATGCCGCAGAGAGATGTTGTGACGTGGAATACAATGATGACTGTGTTGGTGAAGAGAGGTGATGTAGAGGAAGCTTATGATTTGTTTTTGAGGATGCCGGAGAGGAATGTGAGGTCATGGACGTTGATGATTTCGGGGTTTGTGCAGCGCGGTAAGCCCAAGGAGGCGGTTCGTGTGTTTTTGGAGATGGAGGAGGCTGGTGTGAGGGCGAATGAGGTGACTGTGGTGGCGGTTCTCGCGGCGTGTGCTGATTTGGGGGACTTGGATTTGGGTAGGAGAGTTCATGAGTACTCGAGCCGGAGTGGGTTTGGAAGAAATGTTTGGATTTGCAATACTCTGATTGAGATGTATGTGAAATGTGGATGCTTGGAGGATGCACGTAGGGTGTTTGATGCGATGAAAGATCGAACGGTTGTGTCGTGGTCGGCTATGATTTCGGGGCTGGCCATGCATGGACAGGCTGAGGAAGCTTTGAGGCTTTTCTCTAACATGGTTGAGATAGGGATGGATCCAAATCATGTAACTTTTGTTGGACTCTTGCATGCTTGTAGCCACATGGGGTTTGTAGACAAGGGCCGTGAGTTCTTCGAAAGAATGACTGCAGATTATGGAATAGTTCCGAGAATTGAGCATTATGGTTGTATGGTTGATCTTTTAAGTCGGGCAGGGCTCCTTCAAGAGGCTTACGAGTTCATAATGAACATGCCTATAAAACCGAATGGTGTTGTGTGGGGAGCTCTCCTTGGTGGATGCAAAGTTCACAGAAACATTGAACTGGCTGAAGTGGCAACTAAGCACCTTGCTGAACTTGATCCACTGAATGATGGATACTACATTGTTTTGTCGAACATTTATGCAGAGGCACAACGGTGGGAAGAAGTGGCGAGTGTGAGAAAGCTAATGAGGGATCGAGGGGTGAAGAAGACACCTGGGTGGAGTTCAATCACAGTAGATGGGGTGGTTCATGAGTTTGTTGCTGGGGATGAAGCCCATCCTCACGCTGAGGAGATCAACCAAATGTGGGAGAAACTACTTGAAAGAATGAGGATGAAGGGTTATGTGCCCAATACTTCAGTTGTTCTACTAGACATGGAAGAGGAACAGAAGGAGAAATTTCTCAGTCGCCATAGCGAGAAATTAGCCCTGGTTTTTGGGCTGATCAAAACAAAGCCTGGAACACCAATTCGAATTATGAAGAATCTTCGTGTTTGTGAGGACTGTCATGCTGCTTTGAAACTTGTATCAGAAATTGCGGAGAGAGAGATAGTTGTGCGTGACCGAAACAGGTTCCATTGTTTCAAAAATGGTTATTGTTCCTGCAGGGATTACTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

468

Amino Acids

53.4

Weight (kDa)

6.42

Isoelectric Point (pI)

39.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 27 - 61 4.4e-09 PPR repeat family
PPR PF01535 30 - 60 3.2e-08 PPR repeat
PPR_2 PF13041 59 - 106 1.3e-09 PPR repeat family
PPR PF01535 62 - 91 6.3e-08 PPR repeat
PPR_2 PF13041 160 - 207 2.4e-11 PPR repeat family
PPR PF01535 162 - 191 2.1e-07 PPR repeat
E_motif PF20431 277 - 338 1.9e-21 E motif
Eplus_motif PF20430 344 - 371 2.5e-06 E+ motif
DYW_deaminase PF14432 375 - 467 5e-41 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 636
AccII CGCG 2 cut(s) 219, 308
AciI CCGC 6 cut(s) 74, 219, 236, 299, 308, 1322
AclWI GGATC 4 cut(s) 572, 585, 875, 984
AcoI YGGCCR 1 cut(s) 513
AcsI RAATTY 2 cut(s) 16, 1181
AcuI CTGAAG 2 cut(s) 870, 1125
AfaI GTAC 1 cut(s) 359
AfiI CCNNNNNNNGG 2 cut(s) 748, 1090
AgsI TTSAA 6 cut(s) 746, 842, 1011, 1106, 1303, 1369
AjiI CACGTC 1 cut(s) 93
AjnI CCWGG 3 cut(s) 997, 1212, 1240
AjuI GAANNNNNNNTTGG 2 cut(s) 373, 405
AleI CACNNNNGTG 2 cut(s) 937, 1000
AloI GAACNNNNNNTCC 2 cut(s) 810, 842
AluBI AGCT 4 cut(s) 143, 539, 806, 967
AluI AGCT 4 cut(s) 143, 539, 806, 967
Alw21I GWGCWC 1 cut(s) 808
AlwI GGATC 4 cut(s) 572, 585, 875, 984
Ama87I CYCGRG 1 cut(s) 361
AoxI GGCC 2 cut(s) 513, 643
ApeKI GCWGC 2 cut(s) 214, 1295
ApoI RAATTY 2 cut(s) 16, 1181
Asp700I GAANNNNTTC 1 cut(s) 59
AspLEI GCGC 1 cut(s) 219
AspS9I GGNCC 1 cut(s) 643
AsuHPI GGTGA 4 cut(s) 130, 140, 298, 997
AsuII TTCGAA 2 cut(s) 657, 1255
AvaI CYCGRG 1 cut(s) 361
BaeGI GKGCMC 1 cut(s) 1134
BalI TGGCCA 1 cut(s) 515
BamHI GGATCC 1 cut(s) 577
BanII GRGCYC 2 cut(s) 741, 808
BbsI GAAGAC 1 cut(s) 998
Bbv12I GWGCWC 1 cut(s) 808
BbvCI CCTCAGC 2 cut(s) 531, 1071
BbvI GCAGC 2 cut(s) 226, 1282
BccI CCATC 5 cut(s) 250, 568, 887, 1016, 1068
BceAI ACGGC 1 cut(s) 630
BciT130I CCWGG 3 cut(s) 999, 1214, 1242
BciVI GTATCC 1 cut(s) 890
BclI TGATCA 1 cut(s) 1227
BfaI CTAG 1 cut(s) 1154
BfmI CTRYAG 2 cut(s) 669, 1387
BfuI GTATCC 1 cut(s) 890
BisI GCNGC 4 cut(s) 74, 215, 309, 1296
BlsI GCNGC 4 cut(s) 75, 216, 310, 1297
BmcAI AGTACT 1 cut(s) 359
Bme1390I CCNGG 3 cut(s) 999, 1214, 1242
BmeT110I CYCGRG 1 cut(s) 361
BmgBI CACGTC 1 cut(s) 93
BmgT120I GGNCC 1 cut(s) 643
BmiI GGNNCC 3 cut(s) 579, 740, 1358
BmrFI CCNGG 3 cut(s) 999, 1214, 1242
BmsI GCATC 6 cut(s) 37, 153, 422, 433, 451, 809
BpiI GAAGAC 1 cut(s) 998
Bpu10I CCTNAGC 2 cut(s) 531, 1071
Bpu14I TTCGAA 2 cut(s) 657, 1255
BsaAI YACGTR 1 cut(s) 449
BsaBI GATNNNNATC 1 cut(s) 582
BsaJI CCNNGG 4 cut(s) 228, 811, 998, 1212
Bsc4I CCNNNNNNNGG 2 cut(s) 748, 1090
Bse1I ACTGG 2 cut(s) 849, 1403
Bse8I GATNNNNATC 1 cut(s) 582
BseBI CCWGG 3 cut(s) 999, 1214, 1242
BseDI CCNNGG 4 cut(s) 228, 811, 998, 1212
BseGI GGATG 9 cut(s) 52, 168, 437, 448, 579, 824, 1057, 1060, 1122
BseJI GATNNNNATC 1 cut(s) 582
BseLI CCNNNNNNNGG 2 cut(s) 748, 1090
BseMII CTCAG 3 cut(s) 522, 1062, 1201
BseNI ACTGG 2 cut(s) 849, 1403
BseRI GAGGAG 2 cut(s) 275, 1088
BseSI GKGCMC 1 cut(s) 1134
BseXI GCAGC 2 cut(s) 226, 1282
BseYI CCCAGC 1 cut(s) 1046
BsgI GTGCAG 1 cut(s) 233
Bsh1236I CGCG 2 cut(s) 219, 308
BshFI GGCC 2 cut(s) 515, 645
BsiHKAI GWGCWC 1 cut(s) 808
BsiHKCI CYCGRG 1 cut(s) 361
BsiSI CCGG 2 cut(s) 167, 367
BslFI GGGAC 1 cut(s) 341
BslI CCNNNNNNNGG 2 cut(s) 748, 1090
BsmFI GGGAC 1 cut(s) 341
BsnI GGCC 2 cut(s) 515, 645
BsoBI CYCGRG 1 cut(s) 361
Bsp119I TTCGAA 2 cut(s) 657, 1255
Bsp1286I GDGCHC 3 cut(s) 741, 808, 1134
Bsp143I GATC 7 cut(s) 472, 577, 718, 880, 976, 1077, 1227
BspACI CCGC 6 cut(s) 74, 219, 236, 299, 308, 1322
BspANI GGCC 2 cut(s) 515, 645
BspCNI CTCAG 3 cut(s) 523, 1063, 1200
BspFNI CGCG 2 cut(s) 219, 308
BspHI TCATGA 2 cut(s) 352, 1033
BspLI GGNNCC 3 cut(s) 579, 740, 1358
BspMAI CTGCAG 2 cut(s) 673, 1391
BspPI GGATC 4 cut(s) 572, 585, 875, 984
BspT104I TTCGAA 2 cut(s) 657, 1255
BsrI ACTGG 2 cut(s) 849, 1403
BssECI CCNNGG 4 cut(s) 228, 811, 998, 1212
BssMI GATC 7 cut(s) 472, 577, 718, 880, 976, 1077, 1227
BssT1I CCWWGG 2 cut(s) 228, 811
Bst2UI CCWGG 3 cut(s) 999, 1214, 1242
Bst4CI ACNGT 6 cut(s) 112, 292, 481, 939, 1018, 1289
Bst6I CTCTTC 2 cut(s) 116, 1158
BstBAI YACGTR 1 cut(s) 449
BstBI TTCGAA 2 cut(s) 657, 1255
BstC8I GCNNGC 2 cut(s) 513, 612
BstDEI CTNAG 4 cut(s) 531, 861, 1071, 1187
BstENI CCTNNNNNAGG 1 cut(s) 746
BstF5I GGATG 9 cut(s) 52, 168, 437, 448, 579, 824, 1057, 1060, 1122
BstFNI CGCG 2 cut(s) 219, 308
BstHHI GCGC 1 cut(s) 219
BstKTI GATC 7 cut(s) 475, 580, 721, 883, 979, 1080, 1230
BstMBI GATC 7 cut(s) 472, 577, 718, 880, 976, 1077, 1227
BstMWI GCNNNNNNNGC 6 cut(s) 223, 305, 314, 517, 536, 854
BstNI CCWGG 3 cut(s) 999, 1214, 1242
BstNSI RCATGY 2 cut(s) 614, 775
BstSCI CCNGG 3 cut(s) 997, 1212, 1240
BstSFI CTRYAG 2 cut(s) 669, 1387
BstSLI GKGCMC 1 cut(s) 1134
BstUI CGCG 2 cut(s) 219, 308
BstV1I GCAGC 2 cut(s) 226, 1282
BstV2I GAAGAC 1 cut(s) 998
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
BsuI GTATCC 1 cut(s) 890
BsuRI GGCC 2 cut(s) 515, 645
BtgZI GCGATG 1 cut(s) 479
BtrI CACGTC 1 cut(s) 93
BtsCI GGATG 9 cut(s) 52, 168, 437, 448, 579, 824, 1057, 1060, 1122
BtsIMutI CAGTG 1 cut(s) 884
Cac8I GCNNGC 2 cut(s) 513, 612
CciI TCATGA 2 cut(s) 352, 1033
CfoI GCGC 1 cut(s) 219
Cfr13I GGNCC 1 cut(s) 643
Csp6I GTAC 1 cut(s) 358
CviQI GTAC 1 cut(s) 358
DdeI CTNAG 4 cut(s) 531, 861, 1071, 1187
DpnI GATC 7 cut(s) 474, 579, 720, 882, 978, 1079, 1229
DpnII GATC 7 cut(s) 472, 577, 718, 880, 976, 1077, 1227
EaeI YGGCCR 1 cut(s) 513
Eam1104I CTCTTC 2 cut(s) 116, 1158
EarI CTCTTC 2 cut(s) 116, 1158
Ecl136II GAGCTC 1 cut(s) 806
Eco130I CCWWGG 2 cut(s) 228, 811
Eco24I GRGCYC 2 cut(s) 741, 808
Eco53kI GAGCTC 1 cut(s) 806
Eco57I CTGAAG 2 cut(s) 870, 1125
Eco88I CYCGRG 1 cut(s) 361
EcoICRI GAGCTC 1 cut(s) 806
EcoNI CCTNNNNNAGG 1 cut(s) 746
EcoRII CCWGG 3 cut(s) 997, 1212, 1240
EcoT14I CCWWGG 2 cut(s) 228, 811
EcoT22I ATGCAT 1 cut(s) 522
EcoT38I GRGCYC 2 cut(s) 741, 808
ErhI CCWWGG 2 cut(s) 228, 811
FaqI GGGAC 1 cut(s) 341
FbaI TGATCA 1 cut(s) 1227
FblI GTMKAC 1 cut(s) 636
Fnu4HI GCNGC 4 cut(s) 74, 215, 309, 1296
FokI GGATG 9 cut(s) 59, 175, 444, 455, 586, 831, 1047, 1064, 1129
FriOI GRGCYC 2 cut(s) 741, 808
Fsp4HI GCNGC 4 cut(s) 74, 215, 309, 1296
FspBI CTAG 1 cut(s) 1154
GlaI GCGC 1 cut(s) 218
GluI GCNGC 4 cut(s) 74, 215, 309, 1296
GsaI CCCAGC 1 cut(s) 1050
HaeIII GGCC 2 cut(s) 515, 645
HapII CCGG 2 cut(s) 167, 367
HhaI GCGC 1 cut(s) 219
Hin6I GCGC 1 cut(s) 217
HinP1I GCGC 1 cut(s) 217
HindIII AAGCTT 2 cut(s) 141, 537
HinfI GANTC 3 cut(s) 10, 603, 1267
HpaII CCGG 2 cut(s) 167, 367
HphI GGTGA 4 cut(s) 130, 140, 298, 997
Hpy166II GTNNAC 2 cut(s) 637, 829
Hpy188I TCNGA 4 cut(s) 15, 408, 690, 1315
Hpy188III TCNNGA 3 cut(s) 353, 746, 1034
Hpy8I GTNNAC 2 cut(s) 637, 829
HpyAV CCTTC 3 cut(s) 752, 1114, 1168
HpyCH4III ACNGT 6 cut(s) 112, 292, 481, 939, 1018, 1289
HpyCH4IV ACGT 3 cut(s) 92, 191, 448
HpyF10VI GCNNNNNNNGC 6 cut(s) 223, 305, 314, 517, 536, 854
HpyF3I CTNAG 4 cut(s) 531, 861, 1071, 1187
HpySE526I ACGT 3 cut(s) 92, 191, 448
HspAI GCGC 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 1227
Kzo9I GATC 7 cut(s) 472, 577, 718, 880, 976, 1077, 1227
LmnI GCTCC 2 cut(s) 744, 803
Lsp1109I GCAGC 2 cut(s) 226, 1282
LweI GCATC 6 cut(s) 37, 153, 422, 433, 451, 809
MaeI CTAG 1 cut(s) 1154
MaeII ACGT 3 cut(s) 92, 191, 448
MaeIII GTNAC 4 cut(s) 88, 286, 589, 1343
MalI GATC 7 cut(s) 474, 579, 720, 882, 978, 1079, 1229
MboI GATC 7 cut(s) 472, 577, 718, 880, 976, 1077, 1227
MflI RGATCY 1 cut(s) 577
MhlI GDGCHC 3 cut(s) 741, 808, 1134
MlsI TGGCCA 1 cut(s) 515
MluCI AATT 7 cut(s) 16, 693, 1181, 1205, 1251, 1257, 1317
MluNI TGGCCA 1 cut(s) 515
MlyI GAGTC 1 cut(s) 597
MmeI TCCRAC 1 cut(s) 580
Mox20I TGGCCA 1 cut(s) 515
Mph1103I ATGCAT 1 cut(s) 522
MroXI GAANNNNTTC 1 cut(s) 59
MscI TGGCCA 1 cut(s) 515
MseI TTAA 1 cut(s) 725
MslI CAYNNNNRTG 2 cut(s) 937, 1000
Msp20I TGGCCA 1 cut(s) 515
MspI CCGG 2 cut(s) 167, 367
MspR9I CCNGG 3 cut(s) 999, 1214, 1242
MvaI CCWGG 3 cut(s) 999, 1214, 1242
MvnI CGCG 2 cut(s) 219, 308
MwoI GCNNNNNNNGC 6 cut(s) 223, 305, 314, 517, 536, 854
NdeII GATC 7 cut(s) 472, 577, 718, 880, 976, 1077, 1227
NlaIV GGNNCC 3 cut(s) 579, 740, 1358
NmuCI GTSAC 3 cut(s) 88, 286, 1343
NsiI ATGCAT 1 cut(s) 522
NspI RCATGY 2 cut(s) 614, 775
NspV TTCGAA 2 cut(s) 657, 1255
OliI CACNNNNGTG 2 cut(s) 937, 1000
PaeI GCATGC 1 cut(s) 614
PaeR7I CTCGAG 1 cut(s) 361
PagI TCATGA 2 cut(s) 352, 1033
PcsI WCGNNNNNNNCGW 1 cut(s) 485
PdmI GAANNNNTTC 1 cut(s) 59
PfeI GAWTC 2 cut(s) 10, 1267
PkrI GCNGC 4 cut(s) 75, 216, 310, 1297
PleI GAGTC 1 cut(s) 597
PpsI GAGTC 1 cut(s) 597
Ppu21I YACGTR 1 cut(s) 449
Psp124BI GAGCTC 1 cut(s) 808
Psp6I CCWGG 3 cut(s) 997, 1212, 1240
PspFI CCCAGC 1 cut(s) 1046
PspGI CCWGG 3 cut(s) 997, 1212, 1240
PspN4I GGNNCC 3 cut(s) 579, 740, 1358
PspPI GGNCC 1 cut(s) 643
PspXI VCTCGAGB 1 cut(s) 361
PstI CTGCAG 2 cut(s) 673, 1391
PsuI RGATCY 1 cut(s) 577
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
RseI CAYNNNNRTG 2 cut(s) 937, 1000
SacI GAGCTC 1 cut(s) 808
SaqAI TTAA 1 cut(s) 725
SatI GCNGC 4 cut(s) 74, 215, 309, 1296
Sau3AI GATC 7 cut(s) 472, 577, 718, 880, 976, 1077, 1227
Sau96I GGNCC 1 cut(s) 643
SbfI CCTGCAGG 1 cut(s) 1391
ScaI AGTACT 1 cut(s) 359
SchI GAGTC 1 cut(s) 597
ScrFI CCNGG 3 cut(s) 999, 1214, 1242
SdaI CCTGCAGG 1 cut(s) 1391
SduI GDGCHC 3 cut(s) 741, 808, 1134
SfaNI GCATC 6 cut(s) 37, 153, 422, 433, 451, 809
SfcI CTRYAG 2 cut(s) 669, 1387
Sfr274I CTCGAG 1 cut(s) 361
SfuI TTCGAA 2 cut(s) 657, 1255
SlaI CTCGAG 1 cut(s) 361
SmiMI CAYNNNNRTG 2 cut(s) 937, 1000
SmlI CTYRAG 1 cut(s) 361
SmoI CTYRAG 1 cut(s) 361
SphI GCATGC 1 cut(s) 614
Sse8387I CCTGCAGG 1 cut(s) 1391
Sse9I AATT 7 cut(s) 16, 693, 1181, 1205, 1251, 1257, 1317
SsiI CCGC 6 cut(s) 74, 219, 236, 299, 308, 1322
SspMI CTAG 1 cut(s) 1154
SstI GAGCTC 1 cut(s) 808
StyD4I CCNGG 3 cut(s) 997, 1212, 1240
StyI CCWWGG 2 cut(s) 228, 811
TaaI ACNGT 6 cut(s) 112, 292, 481, 939, 1018, 1289
TaiI ACGT 3 cut(s) 95, 194, 451
TaqI TCGA 7 cut(s) 63, 362, 475, 657, 914, 979, 1255
TaqII GACCGA 1 cut(s) 1362
TasI AATT 7 cut(s) 16, 693, 1181, 1205, 1251, 1257, 1317
TatI WGTACW 1 cut(s) 357
TauI GCSGC 2 cut(s) 76, 311
TfiI GAWTC 2 cut(s) 10, 1267
Tru1I TTAA 1 cut(s) 725
Tru9I TTAA 1 cut(s) 725
TscAI CASTG 1 cut(s) 891
TseFI GTSAC 3 cut(s) 88, 286, 1343
TseI GCWGC 2 cut(s) 214, 1295
Tsp45I GTSAC 3 cut(s) 88, 286, 1343
TspDTI ATGAA 9 cut(s) 17, 341, 482, 751, 782, 1022, 1068, 1133, 1277
TspGWI ACGGA 1 cut(s) 67
TspRI CASTG 1 cut(s) 891
XagI CCTNNNNNAGG 1 cut(s) 746
XapI RAATTY 2 cut(s) 16, 1181
XceI RCATGY 2 cut(s) 614, 775
XhoI CTCGAG 1 cut(s) 361
XmiI GTMKAC 1 cut(s) 636
XmnI GAANNNNTTC 1 cut(s) 59
XspI CTAG 1 cut(s) 1154
ZrmI AGTACT 1 cut(s) 359
Zsp2I ATGCAT 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.