RLG00000019432

DYW family of nucleic acid deaminases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007750
Physical Location & Seq
Reverse (-)
51973349 .. 51980350
7002 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019432

Sequence Viewer

Length: 3732 bp
ATGGACATAAGTAATAGGTCAAATTTGTCCATCGGAAGGATGGTCCTCGGTGTTGCCCCTATGCTACATGCCACCTTCTCTGATTTCAGAGTAGGAGGGAGAGGCCAATTCCTCATCCTCGGGGCTGCGATTCTAGGGACTGCACTTGCTTTCTTGGAGATGGAAACTCCTACTACCCCAACTGATAATAATGGGATACCCGGTTCAGAAAGCCAGCCGACTAAGCGCAGAAGAAAGAAGTCTATTGTCTGGGAGCACTTCACAATCGAAACTGTTGGGGCCGGATGTACTAGGGCTTGCTGTAACCAGTGCAAAAGATCTTTTGCATATATTACTGGTTCAAGGATGGCAGGCACAAGCCACTTAAAGCGACATATTTCATTGGGAATTTGCCCAGTAAGCCGTCAGAAAAACCAGATGACTCCTTATACACCTGGCTCCAAAACTGATGCTACTGAGAACCTGGGCTATGTTTTCTTAACAGGACATTTTGTTGATAGTGACTGGAATTCATACAGTCAGATACTCAATGTGGTTATGGTACCATCTCCTGATTCAGGTGACACCTTTAGTCAAGCTATTTTGACTTGCTTGTCCGATTGGCATGTTGAGGGCCGGTTGTTCACCATCACACTTGACCAATCCCTCTCCAATGAAACTATAATTGGAAATCTGAAAGGGCTCCTTTCTGTCAAGAACCCACACATGCTCAACAGTCAATTATTGATAAGAAATTGCTATGCTCGTGTTCTTGGTTGTTTGGCACAAGATATGCTAGGGGCATTGAGTGAAATAATCATGAAAGTTCGCGAAAGCGTTAAGTTTGTGAAGACTTCAGAATCGCATGAAGAAAAATTTGTTCAGCTGAAGCAACAGCTTCAAGTTCCTAGCACAAAAAAGCTTTCAATCGATGATCGTACTAAATGGGACACAACCTACCATATGCTGGTGTCTGCTTGTGAAATGAGGGAAGTATTTGCTTGCTTCGATACATATGATTCTGATTACAAGATAATTATATCATTGGAAGAATGGAGTCAGGTAGAAACTCTATGTACATATGTGAAGTACTTGTTTGATGCAGCTAACGTTGTGGCTGAGCCAGCATACCCAACTGCAAATGTGTTCTTCCCAATAATATCGCAAATTCAAGTTGAACTGATGCAGGCAGCCGTGAGTGAGGACTCATTTGTTAGCTCTTTGATCAGACCTCTGTATGAAAGGTTTGACAGGTATTGGGAAAATTGCTGCCTTGTTTTAGCAATTGCTGTGATTATGGATCCGAGGTACAAAAAACAATTTGTAGATTTCACCTTCTCCAAAATTTATGGGGAGAACGCTGAGACTTGGATCAGATTAGTTGACGATGGACTTCATGAACTCTTCCTCGAGTATATGATGCGAATGCTTAGTCTACCGGAAACATTAATGGTGGAAGGGAATGAGGACATCATCAGATCTGACCCACCTGAAGCAGCAGAATACCTGCAGTATCGAATGTCATTCTCGCTTGCCTCCAAATGGCTCCACTCTTTAACCTTCATGAAAATGGTCTCTGTGGCAGCTTCGGCTCCAACCCACGTGGGTACACTGACCCAGTTTCATTCTTTAGAAAACCCGCCAATTACACACTTTGAAAATTGCAAATCCATGGATCAACTGAAGCAAATGCATGCTCAAACCATTAAAACAGGTTTCGCATCGGACCCGGCCGCGCTCAACAGAGTCATAGTCTTCTGTTGTACCCATGAATTTGGTGACATGAAGTATGCACGCCAGGTGTTTGATAGAATTCCTGAACCAAGTGTTTTCATTTGTAACACCATGATCAGAGGCTATAGTAGGATACATTGCCCCCGTTATGGGGTATCTATGTATTCAGCAATGCAGAGGATGGGTGTGAAGCCCGATTGCTATACATTTCCGTTCTTATTGAGGGGATTTTCTCCTGAGATTGCATTGGAATGGGGGAGAGGCTTCCATGCTCATGTGTTGAAGTATGGTTTCGATTCTAATGTTTTCGTTCACAATGCGTTGGTGCAGATGTACTCATTTTGTGGTCAAGTTGATATGGCACGTGGGGTTTTTGACATGGTCAGTGAGAAGGATGTTGCTACTTGGAATGCAATGATCTCCGGGTACAACAAGATCAGGGAGTTCGATGAGTCATGGAAGCTTTTCTGTGACATGGAGAAGAAGGGAGTATTGCCCACTTCTGTTACCCTTGTCTCGGTACTTTCAGCTTGCTCTAAATTGAAGGATTTAGATGCTGGGAAGCAGGTTCACAACTATCTCAAGGAGTGCGTGATCGAACCTAATCTAGTTTTGGAGAATGCTTTGATTGACATATATGCTGCTTGTGGGAAGACGGATGTTGCACTTGAGATTTTTGAGAATATGAAAAGGAGGGATGTAATTTCTTGGACTATCATTGTTAAGGGGTATTGCAATTCAGGACAAGTTGATTTAGCTCGAAACTACTTTGATCAGATGCCAGACAGAGACTATATTTCATGGACCGCAATAATTGATGGGTACGTAAGGGTGAACCGGTTCAAAGAGGTTTTGGAACTCTTCCGTGAGATGCAGACTTCAAATATAAAACCAGATGAGTATACCATGGTAAGCATTCTAACAGCTTGTGCACATCTTGGAGCACTAGAACTCGGAGAATGGATCGAAACTTACATTGACAAAAACAAGATCAAGAATGACACGTTTGTGCGAAATGCTTTAGTAGACATGTATTTCAAGTGTGGAAATGTTGAAAAAGCTGTCAGGGTATTCAATGCAATGCTTCAAAGAGACAAATTTACCTGGACAGCTATGATTGTTGGCCTTGCTATTAATGGACATGGTAAGGAAGCTCTTGACATGTTCAGTCAAATGCTGAAATCTGCAGTAGCTCCGGACAAGATAACATACCTGGGTGTTCTTTGTGCGTGTACTCACTCTGGCATGGTTGATGAAGGTAGAAAGTTTTTCTCTAGTATGATCACCCTACATGGGATTAATCCTACTGTTGAGCATTACGGGTGCATGGTGGATCTTCTTGGTCGAGCAGGGCATCTTAAAGAAGCTTATGAGGTTATACAGAACATGCCAATAGAGCCAAACTCAGTTGTATGGGGAACTCTTCTCGGTGCTTGTAGAATGCATAAAGATGCAGAACTGGCTGAAGTGGTGGCAAAACAGATACTAGATTTGGATCCTGAAAACAGCGCTGTTTATGTTCTCTTATGCAATATATATGCAGCTTGCAACAAATGGGAAAGTTTCCGCGAGGTAAGGCAAACGATTATGGATAGGGGGATCAAGAAGACTCCTGGTTGTAGTTTGATAGAGGTGAATGGTGATGTTCATGAGTTTGTAGCCGGAGATGGCTCACATCCTCAATCCGAAAAGATATATTCCAAGTTGGATGAGATGATTAAAGACTTGAAATCTGCAGGGTATTCACCTGATACGTCCGAGGTTTTTCTTGATGTAGGGGAAGAGAATAAAGAGAGTGCAGTTTATAAGCACAGTGAGAAGTTAGCAATTGCGTTTGGATTGATTAGTTTAGGACCTGGGGTGACTATTAGAGTCATGAAGAACCTCAGAATCTGTGTGGATTGTCACAGCATGGCAAAGCTGGTTTCCAAGGTGTATGATAGAGAAGTGATTGTTAGGGATGGAACTCGGTTTCACCATTTCAGACATGGCTTCTGCTCATGTAAAGATTATTGGTAA

Protein Analysis

1244

Amino Acids

140.58

Weight (kDa)

6.15

Isoelectric Point (pI)

34.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
hAT-like_RNase-H PF14372 368 - 465 5.8e-32 hAT-like transposase, RNase-H fold
PPR_2 PF13041 601 - 648 1.3e-08 PPR repeat family
PPR_2 PF13041 702 - 751 2.3e-12 PPR repeat family
PPR PF01535 706 - 735 6.4e-07 PPR repeat
PPR_2 PF13041 835 - 883 2.8e-12 PPR repeat family
PPR PF01535 837 - 867 1.3e-06 PPR repeat
PPR_2 PF13041 936 - 982 4.4e-09 PPR repeat family
E_motif PF20431 1053 - 1115 2.2e-15 E motif
Eplus_motif PF20430 1120 - 1145 2e-07 E+ motif
DYW_deaminase PF14432 1151 - 1243 1.7e-36 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3519
AasI GACNNNNNNGTC 1 cut(s) 592
Acc36I ACCTGC 2 cut(s) 1494, 2269
Acc65I GGTACC 1 cut(s) 541
AccB1I GGYRCC 1 cut(s) 541
AccB7I CCANNNNNTGG 1 cut(s) 946
AccI GTMKAC 3 cut(s) 1414, 2615, 2739
AccII CGCG 3 cut(s) 810, 1715, 3282
AccIII TCCGGA 1 cut(s) 2908
AciI CCGC 4 cut(s) 1619, 1713, 2520, 3280
AclI AACGTT 1 cut(s) 1089
AclWI GGATC 9 cut(s) 1274, 1287, 1358, 1662, 2684, 3054, 3203, 3216, 3320
AcoI YGGCCR 1 cut(s) 1710
AcsI RAATTY 9 cut(s) 22, 387, 508, 854, 1146, 1323, 1751, 1791, 2810
AcuI CTGAAG 5 cut(s) 819, 887, 1491, 1682, 3198
AcvI CACGTG 2 cut(s) 1582, 2078
AfeI AGCGCT 1 cut(s) 3223
AfiI CCNNNNNNNGG 9 cut(s) 36, 557, 946, 1521, 1862, 1863, 1864, 2230, 3006
AflIII ACRYGT 3 cut(s) 2715, 2742, 2874
AgeI ACCGGT 1 cut(s) 2550
AjnI CCWGG 7 cut(s) 433, 462, 1776, 2816, 2925, 3325, 3568
AjuI GAANNNNNNNTTGG 2 cut(s) 648, 680
AleI CACNNNNGTG 1 cut(s) 2720
AloI GAACNNNNNNTCC 2 cut(s) 187, 219
Alw21I GWGCWC 3 cut(s) 258, 2647, 2659
Alw26I GTCTC 5 cut(s) 1337, 1558, 2233, 2496, 2799
Alw44I GTGCAC 1 cut(s) 2643
AlwI GGATC 9 cut(s) 1274, 1287, 1358, 1662, 2684, 3054, 3203, 3216, 3320
AlwNI CAGNNNCTG 1 cut(s) 3606
Ama87I CYCGRG 2 cut(s) 119, 1388
Aor13HI TCCGGA 1 cut(s) 2908
Aor51HI AGCGCT 1 cut(s) 3223
AoxI GGCC 5 cut(s) 103, 279, 613, 1710, 2836
ApaLI GTGCAC 1 cut(s) 2643
ApeKI GCWGC 8 cut(s) 125, 1082, 1169, 1248, 1475, 1562, 2354, 3254
ApoI RAATTY 9 cut(s) 22, 387, 508, 854, 1146, 1323, 1751, 1791, 2810
ArsI GACNNNNNNTTYG 2 cut(s) 1396, 1428
AseI ATTAAT 3 cut(s) 1427, 2847, 3012
AsiGI ACCGGT 1 cut(s) 2550
Asp700I GAANNNNTTC 2 cut(s) 2177, 2552
Asp718I GGTACC 1 cut(s) 541
AspLEI GCGC 3 cut(s) 228, 1717, 3224
AspS9I GGNCC 6 cut(s) 43, 279, 613, 1705, 2517, 3566
AsuC2I CCSGG 3 cut(s) 201, 1709, 2137
AvaI CYCGRG 2 cut(s) 119, 1388
AvaII GGWCC 4 cut(s) 43, 1705, 2517, 3566
BaeGI GKGCMC 1 cut(s) 2647
BamHI GGATCC 2 cut(s) 1279, 3208
BanI GGYRCC 1 cut(s) 541
BanII GRGCYC 1 cut(s) 684
BauI CACGAG 1 cut(s) 744
BbrPI CACGTG 2 cut(s) 1582, 2078
BbsI GAAGAC 4 cut(s) 836, 1726, 2372, 3326
Bbv12I GWGCWC 3 cut(s) 258, 2647, 2659
BbvI GCAGC 8 cut(s) 112, 1094, 1181, 1235, 1487, 1574, 2341, 3266
BceAI ACGGC 2 cut(s) 387, 1157
BciT130I CCWGG 7 cut(s) 435, 464, 1778, 2818, 2927, 3327, 3570
BciVI GTATCC 2 cut(s) 189, 1839
BclI TGATCA 4 cut(s) 1203, 1827, 2485, 2994
BcnI CCSGG 3 cut(s) 201, 1709, 2137
BcoDI GTCTC 5 cut(s) 1337, 1558, 2233, 2496, 2799
BfaI CTAG 8 cut(s) 134, 291, 776, 888, 2321, 2660, 2988, 3200
BfmI CTRYAG 4 cut(s) 1487, 1837, 2898, 3447
BfoI RGCGCY 1 cut(s) 3225
BfuAI ACCTGC 2 cut(s) 1494, 2269
BfuI GTATCC 2 cut(s) 189, 1839
BglII AGATCT 2 cut(s) 317, 1457
BisI GCNGC 9 cut(s) 126, 1083, 1170, 1249, 1476, 1563, 1713, 2355, 3255
BlpI GCTNAGC 1 cut(s) 1098
BlsI GCNGC 9 cut(s) 127, 1084, 1171, 1250, 1477, 1564, 1714, 2356, 3256
BmcAI AGTACT 1 cut(s) 1070
Bme18I GGWCC 4 cut(s) 43, 1705, 2517, 3566
BmeT110I CYCGRG 2 cut(s) 119, 1388
BmgT120I GGNCC 6 cut(s) 43, 279, 613, 1705, 2517, 3566
BmiI GGNNCC 9 cut(s) 280, 439, 543, 683, 1281, 1526, 1572, 1707, 3210
BmrI ACTGGG 2 cut(s) 389, 1591
BmuI ACTGGG 2 cut(s) 389, 1591
BpiI GAAGAC 4 cut(s) 836, 1726, 2372, 3326
BplI GAGNNNNNCTC 2 cut(s) 3101, 3133
Bpu1102I GCTNAGC 1 cut(s) 1098
BpuEI CTTGAG 2 cut(s) 2279, 2402
BpuMI CCSGG 3 cut(s) 201, 1709, 2137
Bsa29I ATCGAT 1 cut(s) 909
BsaAI YACGTR 3 cut(s) 1582, 2078, 2539
BsaBI GATNNNNATC 1 cut(s) 3207
BsaI GGTCTC 1 cut(s) 1558
BsaWI WCCGGW 3 cut(s) 1417, 2550, 2908
Bsc4I CCNNNNNNNGG 9 cut(s) 36, 557, 946, 1521, 1862, 1863, 1864, 2230, 3006
Bse118I RCCGGY 2 cut(s) 615, 2550
Bse1I ACTGG 6 cut(s) 307, 340, 395, 509, 1597, 3177
Bse3DI GCAATG 4 cut(s) 1849, 1890, 2133, 2799
Bse8I GATNNNNATC 1 cut(s) 3207
BseAI TCCGGA 1 cut(s) 2908
BseBI CCWGG 7 cut(s) 435, 464, 1778, 2818, 2927, 3327, 3570
BseCI ATCGAT 1 cut(s) 909
BseJI GATNNNNATC 1 cut(s) 3207
BseLI CCNNNNNNNGG 9 cut(s) 36, 557, 946, 1521, 1862, 1863, 1864, 2230, 3006
BseMI GCAATG 4 cut(s) 1849, 1890, 2133, 2799
BseMII CTCAG 6 cut(s) 447, 1089, 1332, 1941, 3132, 3613
BseNI ACTGG 6 cut(s) 307, 340, 395, 509, 1597, 3177
BseSI GKGCMC 1 cut(s) 2647
BseX3I CGGCCG 1 cut(s) 1710
BseXI GCAGC 8 cut(s) 112, 1094, 1181, 1235, 1487, 1574, 2341, 3266
BseYI CCCAGC 1 cut(s) 2270
BsgI GTGCAG 3 cut(s) 126, 2060, 3531
Bsh1236I CGCG 3 cut(s) 810, 1715, 3282
Bsh1285I CGRYCG 1 cut(s) 1713
BshFI GGCC 5 cut(s) 105, 281, 615, 1712, 2838
BshNI GGYRCC 1 cut(s) 541
BshTI ACCGGT 1 cut(s) 2550
BshVI ATCGAT 1 cut(s) 909
BsiEI CGRYCG 1 cut(s) 1713
BsiHKAI GWGCWC 3 cut(s) 258, 2647, 2659
BsiHKCI CYCGRG 2 cut(s) 119, 1388
BsiSI CCGG 9 cut(s) 201, 282, 616, 1418, 1709, 2136, 2551, 2909, 3375
BslFI GGGAC 2 cut(s) 151, 941
BslI CCNNNNNNNGG 9 cut(s) 36, 557, 946, 1521, 1862, 1863, 1864, 2230, 3006
BsmAI GTCTC 5 cut(s) 1337, 1558, 2233, 2496, 2799
BsmFI GGGAC 2 cut(s) 151, 941
BsmI GAATGC 5 cut(s) 1410, 2128, 2338, 2628, 3159
BsnI GGCC 5 cut(s) 105, 281, 615, 1712, 2838
Bso31I GGTCTC 1 cut(s) 1558
BsoBI CYCGRG 2 cut(s) 119, 1388
Bsp1286I GDGCHC 4 cut(s) 258, 684, 2647, 2659
Bsp13I TCCGGA 1 cut(s) 2908
Bsp1407I TGTACA 1 cut(s) 1055
Bsp1720I GCTNAGC 1 cut(s) 1098
Bsp19I CCATGG 2 cut(s) 1650, 2619
Bsp68I TCGCGA 1 cut(s) 810
BspACI CCGC 4 cut(s) 1619, 1713, 2520, 3280
BspANI GGCC 5 cut(s) 105, 281, 615, 1712, 2838
BspCNI CTCAG 6 cut(s) 448, 1090, 1333, 1942, 3131, 3612
BspDI ATCGAT 1 cut(s) 909
BspEI TCCGGA 1 cut(s) 2908
BspFNI CGCG 3 cut(s) 810, 1715, 3282
BspHI TCATGA 5 cut(s) 798, 1375, 1542, 3361, 3588
BspLI GGNNCC 9 cut(s) 280, 439, 543, 683, 1281, 1526, 1572, 1707, 3210
BspMAI CTGCAG 3 cut(s) 1491, 2902, 3451
BspMI ACCTGC 2 cut(s) 1494, 2269
BspPI GGATC 9 cut(s) 1274, 1287, 1358, 1662, 2684, 3054, 3203, 3216, 3320
BspT107I GGYRCC 1 cut(s) 541
BspTNI GGTCTC 1 cut(s) 1558
BsrDI GCAATG 4 cut(s) 1849, 1890, 2133, 2799
BsrFI RCCGGY 2 cut(s) 615, 2550
BsrGI TGTACA 1 cut(s) 1055
BsrI ACTGG 6 cut(s) 307, 340, 395, 509, 1597, 3177
BssAI RCCGGY 2 cut(s) 615, 2550
BssNAI GTATAC 1 cut(s) 2616
BssSI CACGAG 1 cut(s) 744
BssT1I CCWWGG 3 cut(s) 1650, 2619, 3642
Bst1107I GTATAC 1 cut(s) 2616
Bst2BI CACGAG 1 cut(s) 744
Bst2UI CCWGG 7 cut(s) 435, 464, 1778, 2818, 2927, 3327, 3570
Bst4CI ACNGT 5 cut(s) 274, 518, 716, 3022, 3527
Bst6I CTCTTC 4 cut(s) 1388, 2579, 3141, 3489
BstAUI TGTACA 1 cut(s) 1055
BstBAI YACGTR 3 cut(s) 1582, 2078, 2539
BstDEI CTNAG 8 cut(s) 222, 456, 1098, 1341, 1409, 1950, 3118, 3599
BstDSI CCRYGG 2 cut(s) 1650, 2619
BstENI CCTNNNNNAGG 1 cut(s) 555
BstFNI CGCG 3 cut(s) 810, 1715, 3282
BstH2I RGCGCY 1 cut(s) 3225
BstHHI GCGC 3 cut(s) 228, 1717, 3224
BstMAI GTCTC 5 cut(s) 1337, 1558, 2233, 2496, 2799
BstMCI CGRYCG 1 cut(s) 1713
BstMWI GCNNNNNNNGC 6 cut(s) 223, 399, 1103, 1568, 3109, 3173
BstNI CCWGG 7 cut(s) 435, 464, 1778, 2818, 2927, 3327, 3570
BstNSI RCATGY 7 cut(s) 71, 608, 709, 1676, 2746, 2878, 3103
BstSFI CTRYAG 4 cut(s) 1487, 1837, 2898, 3447
BstSLI GKGCMC 1 cut(s) 2647
BstSNI TACGTA 1 cut(s) 2539
BstUI CGCG 3 cut(s) 810, 1715, 3282
BstV1I GCAGC 8 cut(s) 112, 1094, 1181, 1235, 1487, 1574, 2341, 3266
BstV2I GAAGAC 4 cut(s) 836, 1726, 2372, 3326
BstX2I RGATCY 5 cut(s) 317, 1279, 1457, 3046, 3208
BstXI CCANNNNNNTGG 1 cut(s) 1754
BstYI RGATCY 5 cut(s) 317, 1279, 1457, 3046, 3208
BstZ17I GTATAC 1 cut(s) 2616
BstZI CGGCCG 1 cut(s) 1710
Bsu15I ATCGAT 1 cut(s) 909
BsuI GTATCC 2 cut(s) 189, 1839
BsuRI GGCC 5 cut(s) 105, 281, 615, 1712, 2838
BsuTUI ATCGAT 1 cut(s) 909
BtgI CCRYGG 2 cut(s) 1650, 2619
BtsIMutI CAGTG 4 cut(s) 314, 1589, 2104, 3532
BtuMI TCGCGA 1 cut(s) 810
BveI ACCTGC 2 cut(s) 1494, 2269
CaiI CAGNNNCTG 1 cut(s) 3606
CciI TCATGA 5 cut(s) 798, 1375, 1542, 3361, 3588
CfoI GCGC 3 cut(s) 228, 1717, 3224
Cfr10I RCCGGY 2 cut(s) 615, 2550
Cfr13I GGNCC 6 cut(s) 43, 279, 613, 1705, 2517, 3566
ClaI ATCGAT 1 cut(s) 909
CspAI ACCGGT 1 cut(s) 2550
CspCI CAANNNNNGTGG 2 cut(s) 1563, 1598
DdeI CTNAG 8 cut(s) 222, 456, 1098, 1341, 1409, 1950, 3118, 3599
DrdI GACNNNNNNGTC 1 cut(s) 592
DseDI GACNNNNNNGTC 1 cut(s) 592
EaeI YGGCCR 1 cut(s) 1710
EagI CGGCCG 1 cut(s) 1710
Eam1104I CTCTTC 4 cut(s) 1388, 2579, 3141, 3489
EarI CTCTTC 4 cut(s) 1388, 2579, 3141, 3489
EclXI CGGCCG 1 cut(s) 1710
Eco105I TACGTA 1 cut(s) 2539
Eco130I CCWWGG 3 cut(s) 1650, 2619, 3642
Eco24I GRGCYC 1 cut(s) 684
Eco31I GGTCTC 1 cut(s) 1558
Eco47I GGWCC 4 cut(s) 43, 1705, 2517, 3566
Eco47III AGCGCT 1 cut(s) 3223
Eco52I CGGCCG 1 cut(s) 1710
Eco57I CTGAAG 5 cut(s) 819, 887, 1491, 1682, 3198
Eco72I CACGTG 2 cut(s) 1582, 2078
Eco88I CYCGRG 2 cut(s) 119, 1388
EcoNI CCTNNNNNAGG 1 cut(s) 555
EcoO109I RGGNCCY 1 cut(s) 3566
EcoRI GAATTC 2 cut(s) 508, 1791
EcoRII CCWGG 7 cut(s) 433, 462, 1776, 2816, 2925, 3325, 3568
EcoT14I CCWWGG 3 cut(s) 1650, 2619, 3642
EcoT22I ATGCAT 2 cut(s) 1674, 3159
EcoT38I GRGCYC 1 cut(s) 684
ErhI CCWWGG 3 cut(s) 1650, 2619, 3642
FalI AAGNNNNNCTT 2 cut(s) 669, 701
FaqI GGGAC 2 cut(s) 151, 941
FauI CCCGC 1 cut(s) 1626
FauNDI CATATG 3 cut(s) 942, 994, 1060
FbaI TGATCA 4 cut(s) 1203, 1827, 2485, 2994
FblI GTMKAC 3 cut(s) 1414, 2615, 2739
Fnu4HI GCNGC 9 cut(s) 126, 1083, 1170, 1249, 1476, 1563, 1713, 2355, 3255
FriOI GRGCYC 1 cut(s) 684
Fsp4HI GCNGC 9 cut(s) 126, 1083, 1170, 1249, 1476, 1563, 1713, 2355, 3255
FspBI CTAG 8 cut(s) 134, 291, 776, 888, 2321, 2660, 2988, 3200
GlaI GCGC 3 cut(s) 227, 1716, 3223
GluI GCNGC 9 cut(s) 126, 1083, 1170, 1249, 1476, 1563, 1713, 2355, 3255
GsaI CCCAGC 1 cut(s) 2274
HaeII RGCGCY 1 cut(s) 3225
HaeIII GGCC 5 cut(s) 105, 281, 615, 1712, 2838
HapII CCGG 9 cut(s) 201, 282, 616, 1418, 1709, 2136, 2551, 2909, 3375
HhaI GCGC 3 cut(s) 228, 1717, 3224
Hin6I GCGC 3 cut(s) 226, 1715, 3222
HinP1I GCGC 3 cut(s) 226, 1715, 3222
HincII GTYRAC 1 cut(s) 1363
HindII GTYRAC 1 cut(s) 1363
HindIII AAGCTT 3 cut(s) 899, 2174, 3078
HpaII CCGG 9 cut(s) 201, 282, 616, 1418, 1709, 2136, 2551, 2909, 3375
HpyAV CCTTC 9 cut(s) 30, 85, 1324, 1430, 1549, 2098, 2191, 2251, 2963
HpyCH4III ACNGT 5 cut(s) 274, 518, 716, 3022, 3527
HpyCH4IV ACGT 6 cut(s) 1089, 1581, 2077, 2538, 2717, 3467
HpyF10VI GCNNNNNNNGC 6 cut(s) 223, 399, 1103, 1568, 3109, 3173
HpyF3I CTNAG 8 cut(s) 222, 456, 1098, 1341, 1409, 1950, 3118, 3599
HpySE526I ACGT 6 cut(s) 1089, 1581, 2077, 2538, 2717, 3467
HspAI GCGC 3 cut(s) 226, 1715, 3222
Kpn2I TCCGGA 1 cut(s) 2908
KpnI GGTACC 1 cut(s) 545
Ksp22I TGATCA 4 cut(s) 1203, 1827, 2485, 2994
LmnI GCTCC 7 cut(s) 253, 443, 687, 1530, 1576, 2654, 2911
Lsp1109I GCAGC 8 cut(s) 112, 1094, 1181, 1235, 1487, 1574, 2341, 3266
MaeI CTAG 8 cut(s) 134, 291, 776, 888, 2321, 2660, 2988, 3200
MaeII ACGT 6 cut(s) 1089, 1581, 2077, 2538, 2717, 3467
MaeIII GTNAC 9 cut(s) 302, 500, 560, 1757, 1817, 2183, 2218, 3574, 3617
MfeI CAATTG 2 cut(s) 1263, 3540
MflI RGATCY 5 cut(s) 317, 1279, 1457, 3046, 3208
MhlI GDGCHC 4 cut(s) 258, 684, 2647, 2659
MlyI GAGTC 7 cut(s) 415, 1045, 1178, 1734, 2174, 3316, 3594
MmeI TCCRAC 2 cut(s) 1598, 3399
Mph1103I ATGCAT 2 cut(s) 1674, 3159
MroI TCCGGA 1 cut(s) 2908
MroXI GAANNNNTTC 2 cut(s) 2177, 2552
MslI CAYNNNNRTG 5 cut(s) 1547, 1963, 1986, 2720, 3162
MspA1I CMGCKG 1 cut(s) 865
MspI CCGG 9 cut(s) 201, 282, 616, 1418, 1709, 2136, 2551, 2909, 3375
MunI CAATTG 2 cut(s) 1263, 3540
Mva1269I GAATGC 5 cut(s) 1410, 2128, 2338, 2628, 3159
MvaI CCWGG 7 cut(s) 435, 464, 1778, 2818, 2927, 3327, 3570
MvnI CGCG 3 cut(s) 810, 1715, 3282
MwoI GCNNNNNNNGC 6 cut(s) 223, 399, 1103, 1568, 3109, 3173
NciI CCSGG 3 cut(s) 201, 1709, 2137
NcoI CCATGG 2 cut(s) 1650, 2619
NdeI CATATG 3 cut(s) 942, 994, 1060
NlaIV GGNNCC 9 cut(s) 280, 439, 543, 683, 1281, 1526, 1572, 1707, 3210
NmuCI GTSAC 6 cut(s) 500, 560, 1757, 2183, 3574, 3617
NruI TCGCGA 1 cut(s) 810
NsiI ATGCAT 2 cut(s) 1674, 3159
NspI RCATGY 7 cut(s) 71, 608, 709, 1676, 2746, 2878, 3103
OliI CACNNNNGTG 1 cut(s) 2720
PaeI GCATGC 1 cut(s) 1676
PaeR7I CTCGAG 1 cut(s) 1388
PagI TCATGA 5 cut(s) 798, 1375, 1542, 3361, 3588
PciI ACATGT 2 cut(s) 2742, 2874
PctI GAATGC 5 cut(s) 1410, 2128, 2338, 2628, 3159
PdmI GAANNNNTTC 2 cut(s) 2177, 2552
PfeI GAWTC 6 cut(s) 130, 554, 839, 998, 2009, 3603
PflFI GACNNNGTC 1 cut(s) 2093
PflMI CCANNNNNTGG 1 cut(s) 946
PinAI ACCGGT 1 cut(s) 2550
PkrI GCNGC 9 cut(s) 127, 1084, 1171, 1250, 1477, 1564, 1714, 2356, 3256
PleI GAGTC 7 cut(s) 415, 1044, 1178, 1733, 2173, 3316, 3593
PmaCI CACGTG 2 cut(s) 1582, 2078
PmlI CACGTG 2 cut(s) 1582, 2078
PpsI GAGTC 7 cut(s) 415, 1044, 1178, 1733, 2173, 3316, 3593
Ppu21I YACGTR 3 cut(s) 1582, 2078, 2539
PpuMI RGGWCCY 1 cut(s) 3566
PscI ACATGT 2 cut(s) 2742, 2874
PshBI ATTAAT 3 cut(s) 1427, 2847, 3012
PsiI TTATAA 1 cut(s) 3519
Psp1406I AACGTT 1 cut(s) 1089
Psp5II RGGWCCY 1 cut(s) 3566
Psp6I CCWGG 7 cut(s) 433, 462, 1776, 2816, 2925, 3325, 3568
PspCI CACGTG 2 cut(s) 1582, 2078
PspFI CCCAGC 1 cut(s) 2270
PspGI CCWGG 7 cut(s) 433, 462, 1776, 2816, 2925, 3325, 3568
PspN4I GGNNCC 9 cut(s) 280, 439, 543, 683, 1281, 1526, 1572, 1707, 3210
PspPI GGNCC 6 cut(s) 43, 279, 613, 1705, 2517, 3566
PspPPI RGGWCCY 1 cut(s) 3566
PspXI VCTCGAGB 1 cut(s) 1388
PstI CTGCAG 3 cut(s) 1491, 2902, 3451
PstNI CAGNNNCTG 1 cut(s) 3606
PsuI RGATCY 5 cut(s) 317, 1279, 1457, 3046, 3208
PsyI GACNNNGTC 1 cut(s) 2093
PvuII CAGCTG 1 cut(s) 865
RruI TCGCGA 1 cut(s) 810
RseI CAYNNNNRTG 5 cut(s) 1547, 1963, 1986, 2720, 3162
SatI GCNGC 9 cut(s) 126, 1083, 1170, 1249, 1476, 1563, 1713, 2355, 3255
Sau96I GGNCC 6 cut(s) 43, 279, 613, 1705, 2517, 3566
ScaI AGTACT 1 cut(s) 1070
SchI GAGTC 7 cut(s) 415, 1045, 1178, 1734, 2174, 3316, 3594
SduI GDGCHC 4 cut(s) 258, 684, 2647, 2659
SfcI CTRYAG 4 cut(s) 1487, 1837, 2898, 3447
Sfr274I CTCGAG 1 cut(s) 1388
SinI GGWCC 4 cut(s) 43, 1705, 2517, 3566
SlaI CTCGAG 1 cut(s) 1388
SmiMI CAYNNNNRTG 5 cut(s) 1547, 1963, 1986, 2720, 3162
SmlI CTYRAG 3 cut(s) 1388, 2294, 2381
SmoI CTYRAG 3 cut(s) 1388, 2294, 2381
SnaBI TACGTA 1 cut(s) 2539
SphI GCATGC 1 cut(s) 1676
SsiI CCGC 4 cut(s) 1619, 1713, 2520, 3280
SspMI CTAG 8 cut(s) 134, 291, 776, 888, 2321, 2660, 2988, 3200
StyI CCWWGG 3 cut(s) 1650, 2619, 3642
TaaI ACNGT 5 cut(s) 274, 518, 716, 3022, 3527
TaiI ACGT 6 cut(s) 1092, 1584, 2080, 2541, 2720, 3470
TatI WGTACW 5 cut(s) 287, 1055, 1068, 2046, 2945
TauI GCSGC 1 cut(s) 1715
TfiI GAWTC 6 cut(s) 130, 554, 839, 998, 2009, 3603
TscAI CASTG 4 cut(s) 314, 1596, 2104, 3532
TseFI GTSAC 6 cut(s) 500, 560, 1757, 2183, 3574, 3617
TseI GCWGC 8 cut(s) 125, 1082, 1169, 1248, 1475, 1562, 2354, 3254
Tsp45I GTSAC 6 cut(s) 500, 560, 1757, 2183, 3574, 3617
TspGWI ACGGA 3 cut(s) 1914, 2384, 2567
TspRI CASTG 4 cut(s) 314, 1596, 2104, 3532
Tth111I GACNNNGTC 1 cut(s) 2093
Van91I CCANNNNNTGG 1 cut(s) 946
VneI GTGCAC 1 cut(s) 2643
VpaK11BI GGWCC 4 cut(s) 43, 1705, 2517, 3566
VspI ATTAAT 3 cut(s) 1427, 2847, 3012
XagI CCTNNNNNAGG 1 cut(s) 555
XapI RAATTY 9 cut(s) 22, 387, 508, 854, 1146, 1323, 1751, 1791, 2810
XceI RCATGY 7 cut(s) 71, 608, 709, 1676, 2746, 2878, 3103
XcmI CCANNNNNNNNNTGG 2 cut(s) 37, 3698
XhoI CTCGAG 1 cut(s) 1388
XmiI GTMKAC 3 cut(s) 1414, 2615, 2739
XmnI GAANNNNTTC 2 cut(s) 2177, 2552
XspI CTAG 8 cut(s) 134, 291, 776, 888, 2321, 2660, 2988, 3200
ZrmI AGTACT 1 cut(s) 1070
Zsp2I ATGCAT 2 cut(s) 1674, 3159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.