RchiOBHm_Chr2g0134941

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007750
Physical Location & Seq
Reverse (-)
52052897 .. 52055122
2226 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50592

Sequence Viewer

Length: 2214 bp
ATGGCTCCCTCTTCTTCAACCTTCATGAAAATGGTCTCTGTGGCAGCTTCGGCTCCAACCCACTTGGTTACACATACCCAGTTTCATTCTTTAGAAAACCCACCAGTTACACTCTTTGAAAATTGCAAATCCATGGACCAACTGAAGCAAATGCATGCTCAAACCATTAAAACAGGTTTCGCATCGGACCCGGCCGCGCTCAACAGGATCATAGTCTTCTGTTGTACCCATGAATTTGGTGACATGAAGTACGCACGCCAGGTGTTTGTTAGAATTCCTGAACCAAGTGTTTTTATTTGCAACGCCATGATCAAAGGCTATAGTAGGACACATTGCTCACATTATGGGATATCTATGTATTCAGCAATGCGGAAGATGGGTGTGAAGCCCGATTGCTATACGTTCCCCTTCTTGTTAAGGGGATTTTCTCGTGAGATTGCGTTGAAATGGGGGAGAGGGTTCCATGCTCATGTGTTGAAGTATGGTTTCGATTCTAATGTTTTTGCTCACAATGCGTTGGTGCATATGTACTCTCTTTGTGGTCAAATTGATATGGCTCGTGGGGTTTTCGATATGGTCAGTGACAAGGATGTTGCTACTTGGAATGCAATGATCTCCGGGTACAATAAGATCAAGAAGTTCAATGAGTCATGGAGGCTTTTCCGTGACATGGAGAAGAAGGGAGTGTTTCCCACTTCTGTTACCCTTGTCTCAGTACTTTCAGCTTGCTCCAAATTGAAGGATTTAGATGCTGGGAAGCAGGTTCACAACTACGTCAAGGAATGTGTGGTCGAACCTAATCTAGTTTTGGAGAATGCTTTGATTGACATGTATGCTGCTTGCGGGAAGATGGATGTTGCAATTGAGATTTTTGAGAATATGAAAAGGAGGGATGTAATTTCTTGGACTACCATTGTTAAGGGGTTTTGCAATTCAGGACAAGTTGATTTAGCTCGAGATTACTTTGATCAGATGCCAGACAGAGACTATATTTCATGGACTGCAATAATTGATGGGTACGTAAGGGTGAACCGGTTCAAAGAGGTTTTGGAGCTCTTCCGTGAGATGCAGAGTTCAAATATAAAACCAGATGAGTATACCATGGTAAGCATTCTAACAGCTTGTGCACATCTTGGAGCACTAGAACTCGGAGAATGGATAAGAACTTACATTGACAAAAACAAGATCAAGAATGACACATTTATGCGAAATGCTTTAATAGACATGTATTTCAAGTGTGGAAATGTTGAAAAAGCTGTGAGGGTATTCAATGAAATGCTTCAAAGGGACAAATTTACCTGGACAGCTATGATTGTTGGCCTTGCTATTAACGGACACGGTAAGGAATCTCTTGACATGTTCAGTCAAATGCTGAAATCTACAGTATCTCCGGACAAGATAACATACCTGGGTGTTCTTTGTGCGTGTACTCACTCTGGCATGGTCGAAGAAGGTAGAATGTTTTTCTCTAGTATGATCACCCTACATGGGATTGAGCCTACTGTTGAGCATTACGGGTGCATGGTGGATCTTCTTGGTCGAGCAGGGCGTCTTAAAGAAGCTTATGAGGTTATACAGAACATGCCAATAGAACCAAACTCAGTTGTTTGGGGAACTCTTCTCGGTGCTTGTAGAATGCATAAAGATGCAGAACTGGCTGAAGTGGTGGCAAAACAGATACTAGATTTGGATCCCGAAAACAGCGCTGTTTATGTTCTCTTATGCAATATATATGCAGCTTGCAACAAATGGGAAAGATTCCGCGAGGTAAGGCAAACGATTATGGATAGGGGGATCAAGAAGACTCCTGGTTGTAGTTTGATAGAGGTGAATGGTGATGTTCATGAGTTTGTAGCCGGGGATGTCTCACATCCTCAATCCGAAAAGATATATTCCAAGTTGGATGAGATGATTAAAGACTTGAAATCTGCAGGGTATTCACCTGATACGTCCGAGGTTTTTCTTGATGTAGGGGAAGAGAATAAGGAGAGTGCAGTTTATAAGCACAGTGAGAAGTTAGCAATTGCGTTTGGATTGATTAGTTTAGGACCTGGGGTGACTATTAGAGTCATGAAGAACCTCAGAATCTGCGTGGATTGTCACAGCATGGCAAAGCTGGTTTCCAAGGTGTATGATAGAGAAGTGATTGTTAGGGATGGAACTCGGTTCCACCATTTCAGACATGGCTCATGTTCATGTAAAGATTATTGGTAA

Protein Analysis

737

Amino Acids

83.53

Weight (kDa)

7.51

Isoelectric Point (pI)

33.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 95 - 143 3.2e-09 PPR repeat family
PPR_2 PF13041 196 - 245 7.6e-13 PPR repeat family
PPR PF01535 200 - 229 1.2e-06 PPR repeat
PPR_3 PF13812 219 - 278 7.4e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 298 - 325 7.2e-06 PPR repeat
PPR_2 PF13041 329 - 377 6.3e-13 PPR repeat family
PPR PF01535 331 - 361 3.1e-07 PPR repeat
PPR_2 PF13041 399 - 429 2.8e-06 PPR repeat family
PPR PF01535 404 - 429 3.3e-06 PPR repeat
PPR_2 PF13041 430 - 476 1.1e-07 PPR repeat family
E_motif PF20431 547 - 609 1.8e-15 E motif
Eplus_motif PF20430 614 - 639 9.5e-08 E+ motif
DYW_deaminase PF14432 645 - 737 4.8e-37 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2001
Acc36I ACCTGC 1 cut(s) 751
AccI GTMKAC 1 cut(s) 1097
AccII CGCG 2 cut(s) 197, 1764
AccIII TCCGGA 1 cut(s) 1390
AciI CCGC 4 cut(s) 195, 370, 843, 1762
AclWI GGATC 5 cut(s) 215, 1536, 1685, 1698, 1802
AcoI YGGCCR 1 cut(s) 192
AcsI RAATTY 3 cut(s) 233, 273, 1292
AcuI CTGAAG 2 cut(s) 164, 1680
AcyI GRCGYC 1 cut(s) 1549
AfaI GTAC 7 cut(s) 226, 251, 530, 623, 717, 1019, 1429
AfeI AGCGCT 1 cut(s) 1705
AfiI CCNNNNNNNGG 2 cut(s) 670, 1488
AflIII ACRYGT 3 cut(s) 828, 1224, 1356
AgeI ACCGGT 1 cut(s) 1032
AjnI CCWGG 5 cut(s) 258, 1298, 1407, 1807, 2050
Alw21I GWGCWC 3 cut(s) 1056, 1129, 1141
Alw26I GTCTC 4 cut(s) 40, 715, 978, 1870
Alw44I GTGCAC 1 cut(s) 1125
AlwI GGATC 5 cut(s) 215, 1536, 1685, 1698, 1802
AlwNI CAGNNNCTG 1 cut(s) 2088
Ama87I CYCGRG 1 cut(s) 954
Aor13HI TCCGGA 1 cut(s) 1390
Aor51HI AGCGCT 1 cut(s) 1705
AoxI GGCC 2 cut(s) 192, 1318
ApaLI GTGCAC 1 cut(s) 1125
ApeKI GCWGC 3 cut(s) 44, 836, 1736
ApoI RAATTY 3 cut(s) 233, 273, 1292
AsiGI ACCGGT 1 cut(s) 1032
Asp700I GAANNNNTTC 2 cut(s) 1034, 1278
AspLEI GCGC 2 cut(s) 199, 1706
AspS9I GGNCC 3 cut(s) 136, 187, 2048
AsuC2I CCSGG 3 cut(s) 191, 619, 1858
AsuHPI GGTGA 7 cut(s) 251, 1039, 1471, 1840, 1847, 1932, 2068
AvaI CYCGRG 1 cut(s) 954
AvaII GGWCC 3 cut(s) 136, 187, 2048
BaeGI GKGCMC 1 cut(s) 1129
BamHI GGATCC 1 cut(s) 1690
BanII GRGCYC 1 cut(s) 1056
BauI CACGAG 2 cut(s) 429, 558
BbsI GAAGAC 2 cut(s) 208, 1808
Bbv12I GWGCWC 3 cut(s) 1056, 1129, 1141
BbvI GCAGC 3 cut(s) 56, 823, 1748
BccI CCATC 4 cut(s) 370, 844, 1007, 2150
BciT130I CCWGG 5 cut(s) 260, 1300, 1409, 1809, 2052
BclI TGATCA 3 cut(s) 309, 967, 1476
BcnI CCSGG 3 cut(s) 191, 619, 1858
BcoDI GTCTC 4 cut(s) 40, 715, 978, 1870
BfaI CTAG 4 cut(s) 803, 1142, 1470, 1682
BfmI CTRYAG 3 cut(s) 319, 1380, 1929
BfoI RGCGCY 1 cut(s) 1707
BfuAI ACCTGC 1 cut(s) 751
BisI GCNGC 4 cut(s) 45, 195, 837, 1737
BlsI GCNGC 4 cut(s) 46, 196, 838, 1738
BmcAI AGTACT 1 cut(s) 717
Bme1390I CCNGG 8 cut(s) 191, 260, 619, 1300, 1409, 1809, 1858, 2052
Bme18I GGWCC 3 cut(s) 136, 187, 2048
BmeT110I CYCGRG 1 cut(s) 954
BmgT120I GGNCC 3 cut(s) 136, 187, 2048
BmiI GGNNCC 6 cut(s) 6, 54, 189, 461, 1692, 2168
BmrFI CCNGG 8 cut(s) 191, 260, 619, 1300, 1409, 1809, 1858, 2052
BmrI ACTGGG 1 cut(s) 73
BmsI GCATC 5 cut(s) 191, 739, 963, 1056, 1636
BmuI ACTGGG 1 cut(s) 73
BpiI GAAGAC 2 cut(s) 208, 1808
BpuMI CCSGG 3 cut(s) 191, 619, 1858
BsaAI YACGTR 1 cut(s) 1021
BsaBI GATNNNNATC 1 cut(s) 1689
BsaHI GRCGYC 1 cut(s) 1549
BsaI GGTCTC 1 cut(s) 40
BsaJI CCNNGG 7 cut(s) 132, 1101, 1408, 1857, 1953, 2051, 2124
BsaWI WCCGGW 2 cut(s) 1032, 1390
BsaXI ACNNNNNCTCC 2 cut(s) 1372, 1402
Bsc4I CCNNNNNNNGG 2 cut(s) 670, 1488
Bse118I RCCGGY 1 cut(s) 1032
Bse1I ACTGG 3 cut(s) 79, 104, 1659
Bse3DI GCAATG 3 cut(s) 331, 372, 615
Bse8I GATNNNNATC 1 cut(s) 1689
BseAI TCCGGA 1 cut(s) 1390
BseBI CCWGG 5 cut(s) 260, 1300, 1409, 1809, 2052
BseDI CCNNGG 7 cut(s) 132, 1101, 1408, 1857, 1953, 2051, 2124
BseGI GGATG 7 cut(s) 595, 859, 898, 1867, 1870, 1909, 2161
BseJI GATNNNNATC 1 cut(s) 1689
BseLI CCNNNNNNNGG 2 cut(s) 670, 1488
BseMI GCAATG 3 cut(s) 331, 372, 615
BseMII CTCAG 3 cut(s) 726, 1614, 2095
BseNI ACTGG 3 cut(s) 79, 104, 1659
BseSI GKGCMC 1 cut(s) 1129
BseX3I CGGCCG 1 cut(s) 192
BseXI GCAGC 3 cut(s) 56, 823, 1748
BseYI CCCAGC 1 cut(s) 752
BsgI GTGCAG 1 cut(s) 2013
Bsh1236I CGCG 2 cut(s) 197, 1764
Bsh1285I CGRYCG 1 cut(s) 195
BshFI GGCC 2 cut(s) 194, 1320
BshTI ACCGGT 1 cut(s) 1032
BsiEI CGRYCG 1 cut(s) 195
BsiHKAI GWGCWC 3 cut(s) 1056, 1129, 1141
BsiHKCI CYCGRG 1 cut(s) 954
BsiSI CCGG 5 cut(s) 191, 618, 1033, 1391, 1857
BslFI GGGAC 1 cut(s) 1301
BslI CCNNNNNNNGG 2 cut(s) 670, 1488
BsmAI GTCTC 4 cut(s) 40, 715, 978, 1870
BsmFI GGGAC 1 cut(s) 1301
BsmI GAATGC 4 cut(s) 610, 820, 1110, 1641
BsnI GGCC 2 cut(s) 194, 1320
Bso31I GGTCTC 1 cut(s) 40
BsoBI CYCGRG 1 cut(s) 954
Bsp1286I GDGCHC 3 cut(s) 1056, 1129, 1141
Bsp13I TCCGGA 1 cut(s) 1390
Bsp19I CCATGG 2 cut(s) 132, 1101
BspACI CCGC 4 cut(s) 195, 370, 843, 1762
BspANI GGCC 2 cut(s) 194, 1320
BspCNI CTCAG 3 cut(s) 725, 1613, 2094
BspEI TCCGGA 1 cut(s) 1390
BspFNI CGCG 2 cut(s) 197, 1764
BspHI TCATGA 3 cut(s) 24, 1843, 2070
BspLI GGNNCC 6 cut(s) 6, 54, 189, 461, 1692, 2168
BspMAI CTGCAG 1 cut(s) 1933
BspMI ACCTGC 1 cut(s) 751
BspPI GGATC 5 cut(s) 215, 1536, 1685, 1698, 1802
BspQI GCTCTTC 1 cut(s) 1061
BspTNI GGTCTC 1 cut(s) 40
BsrDI GCAATG 3 cut(s) 331, 372, 615
BsrFI RCCGGY 1 cut(s) 1032
BsrI ACTGG 3 cut(s) 79, 104, 1659
BssAI RCCGGY 1 cut(s) 1032
BssECI CCNNGG 7 cut(s) 132, 1101, 1408, 1857, 1953, 2051, 2124
BssNAI GTATAC 1 cut(s) 1098
BssNI GRCGYC 1 cut(s) 1549
BssSI CACGAG 2 cut(s) 429, 558
BssT1I CCWWGG 3 cut(s) 132, 1101, 2124
Bst1107I GTATAC 1 cut(s) 1098
Bst2BI CACGAG 2 cut(s) 429, 558
Bst2UI CCWGG 5 cut(s) 260, 1300, 1409, 1809, 2052
Bst4CI ACNGT 4 cut(s) 1340, 1384, 1504, 2009
Bst6I CTCTTC 4 cut(s) 16, 1061, 1623, 1971
BstACI GRCGYC 1 cut(s) 1549
BstBAI YACGTR 1 cut(s) 1021
BstC8I GCNNGC 5 cut(s) 156, 256, 727, 841, 1741
BstDEI CTNAG 3 cut(s) 712, 1600, 2081
BstDSI CCRYGG 2 cut(s) 132, 1101
BstF5I GGATG 7 cut(s) 595, 859, 898, 1867, 1870, 1909, 2161
BstFNI CGCG 2 cut(s) 197, 1764
BstH2I RGCGCY 1 cut(s) 1707
BstHHI GCGC 2 cut(s) 199, 1706
BstMAI GTCTC 4 cut(s) 40, 715, 978, 1870
BstMCI CGRYCG 1 cut(s) 195
BstMWI GCNNNNNNNGC 3 cut(s) 50, 512, 1655
BstNI CCWGG 5 cut(s) 260, 1300, 1409, 1809, 2052
BstNSI RCATGY 5 cut(s) 158, 832, 1228, 1360, 1585
BstSCI CCNGG 8 cut(s) 189, 258, 617, 1298, 1407, 1807, 1856, 2050
BstSFI CTRYAG 3 cut(s) 319, 1380, 1929
BstSLI GKGCMC 1 cut(s) 1129
BstSNI TACGTA 1 cut(s) 1021
BstUI CGCG 2 cut(s) 197, 1764
BstV1I GCAGC 3 cut(s) 56, 823, 1748
BstV2I GAAGAC 2 cut(s) 208, 1808
BstX2I RGATCY 2 cut(s) 1528, 1690
BstXI CCANNNNNNTGG 1 cut(s) 236
BstYI RGATCY 2 cut(s) 1528, 1690
BstZ17I GTATAC 1 cut(s) 1098
BstZI CGGCCG 1 cut(s) 192
BsuRI GGCC 2 cut(s) 194, 1320
BtgI CCRYGG 2 cut(s) 132, 1101
BtsCI GGATG 7 cut(s) 595, 859, 898, 1867, 1870, 1909, 2161
BtsIMutI CAGTG 2 cut(s) 586, 2014
BveI ACCTGC 1 cut(s) 751
Cac8I GCNNGC 5 cut(s) 156, 256, 727, 841, 1741
CaiI CAGNNNCTG 1 cut(s) 2088
CciI TCATGA 3 cut(s) 24, 1843, 2070
CfoI GCGC 2 cut(s) 199, 1706
Cfr10I RCCGGY 1 cut(s) 1032
Cfr13I GGNCC 3 cut(s) 136, 187, 2048
CseI GACGC 1 cut(s) 1538
Csp6I GTAC 7 cut(s) 225, 250, 529, 622, 716, 1018, 1428
CspAI ACCGGT 1 cut(s) 1032
CviQI GTAC 7 cut(s) 225, 250, 529, 622, 716, 1018, 1428
DdeI CTNAG 3 cut(s) 712, 1600, 2081
EaeI YGGCCR 1 cut(s) 192
EagI CGGCCG 1 cut(s) 192
Eam1104I CTCTTC 4 cut(s) 16, 1061, 1623, 1971
EarI CTCTTC 4 cut(s) 16, 1061, 1623, 1971
Ecl136II GAGCTC 1 cut(s) 1054
EclXI CGGCCG 1 cut(s) 192
Eco105I TACGTA 1 cut(s) 1021
Eco130I CCWWGG 3 cut(s) 132, 1101, 2124
Eco24I GRGCYC 1 cut(s) 1056
Eco31I GGTCTC 1 cut(s) 40
Eco32I GATATC 1 cut(s) 351
Eco47I GGWCC 3 cut(s) 136, 187, 2048
Eco47III AGCGCT 1 cut(s) 1705
Eco52I CGGCCG 1 cut(s) 192
Eco53kI GAGCTC 1 cut(s) 1054
Eco57I CTGAAG 2 cut(s) 164, 1680
Eco88I CYCGRG 1 cut(s) 954
EcoICRI GAGCTC 1 cut(s) 1054
EcoO109I RGGNCCY 1 cut(s) 2048
EcoRI GAATTC 1 cut(s) 273
EcoRII CCWGG 5 cut(s) 258, 1298, 1407, 1807, 2050
EcoRV GATATC 1 cut(s) 351
EcoT14I CCWWGG 3 cut(s) 132, 1101, 2124
EcoT22I ATGCAT 2 cut(s) 156, 1641
EcoT38I GRGCYC 1 cut(s) 1056
ErhI CCWWGG 3 cut(s) 132, 1101, 2124
FaqI GGGAC 1 cut(s) 1301
FauI CCCGC 1 cut(s) 836
FauNDI CATATG 1 cut(s) 525
FbaI TGATCA 3 cut(s) 309, 967, 1476
FblI GTMKAC 1 cut(s) 1097
Fnu4HI GCNGC 4 cut(s) 45, 195, 837, 1737
FokI GGATG 7 cut(s) 602, 866, 905, 1857, 1874, 1916, 2168
FriOI GRGCYC 1 cut(s) 1056
Fsp4HI GCNGC 4 cut(s) 45, 195, 837, 1737
FspBI CTAG 4 cut(s) 803, 1142, 1470, 1682
GlaI GCGC 2 cut(s) 198, 1705
GluI GCNGC 4 cut(s) 45, 195, 837, 1737
GsaI CCCAGC 1 cut(s) 756
HaeII RGCGCY 1 cut(s) 1707
HaeIII GGCC 2 cut(s) 194, 1320
HapII CCGG 5 cut(s) 191, 618, 1033, 1391, 1857
HgaI GACGC 1 cut(s) 1538
HhaI GCGC 2 cut(s) 199, 1706
Hin1I GRCGYC 1 cut(s) 1549
Hin6I GCGC 2 cut(s) 197, 1704
HinP1I GCGC 2 cut(s) 197, 1704
HindIII AAGCTT 1 cut(s) 1560
HinfI GANTC 7 cut(s) 491, 647, 1346, 1758, 1804, 2067, 2085
HpaII CCGG 5 cut(s) 191, 618, 1033, 1391, 1857
HphI GGTGA 7 cut(s) 251, 1039, 1471, 1840, 1847, 1932, 2068
Hpy166II GTNNAC 5 cut(s) 766, 1030, 1098, 1127, 1428
Hpy188I TCNGA 7 cut(s) 187, 972, 1151, 1882, 1954, 2084, 2180
Hpy8I GTNNAC 5 cut(s) 766, 1030, 1098, 1127, 1428
HpyAV CCTTC 5 cut(s) 31, 418, 673, 733, 1445
HpyCH4III ACNGT 4 cut(s) 1340, 1384, 1504, 2009
HpyCH4IV ACGT 4 cut(s) 401, 774, 1020, 1949
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 512, 1655
HpyF3I CTNAG 3 cut(s) 712, 1600, 2081
HpySE526I ACGT 4 cut(s) 401, 774, 1020, 1949
Hsp92I GRCGYC 1 cut(s) 1549
HspAI GCGC 2 cut(s) 197, 1704
Kpn2I TCCGGA 1 cut(s) 1390
Ksp22I TGATCA 3 cut(s) 309, 967, 1476
LguI GCTCTTC 1 cut(s) 1061
LmnI GCTCC 5 cut(s) 10, 58, 734, 1051, 1136
Lsp1109I GCAGC 3 cut(s) 56, 823, 1748
LweI GCATC 5 cut(s) 191, 739, 963, 1056, 1636
MaeI CTAG 4 cut(s) 803, 1142, 1470, 1682
MaeII ACGT 4 cut(s) 401, 774, 1020, 1949
MaeIII GTNAC 8 cut(s) 67, 106, 239, 581, 665, 700, 2056, 2099
MfeI CAATTG 2 cut(s) 861, 2022
MflI RGATCY 2 cut(s) 1528, 1690
MhlI GDGCHC 3 cut(s) 1056, 1129, 1141
MlyI GAGTC 3 cut(s) 656, 1798, 2076
MmeI TCCRAC 2 cut(s) 80, 1881
Mph1103I ATGCAT 2 cut(s) 156, 1641
MroI TCCGGA 1 cut(s) 1390
MroXI GAANNNNTTC 2 cut(s) 1034, 1278
MseI TTAA 7 cut(s) 168, 416, 918, 1217, 1329, 1554, 1914
MslI CAYNNNNRTG 5 cut(s) 29, 468, 1202, 1644, 2194
MspI CCGG 5 cut(s) 191, 618, 1033, 1391, 1857
MspR9I CCNGG 8 cut(s) 191, 260, 619, 1300, 1409, 1809, 1858, 2052
MunI CAATTG 2 cut(s) 861, 2022
Mva1269I GAATGC 4 cut(s) 610, 820, 1110, 1641
MvaI CCWGG 5 cut(s) 260, 1300, 1409, 1809, 2052
MvnI CGCG 2 cut(s) 197, 1764
MwoI GCNNNNNNNGC 3 cut(s) 50, 512, 1655
NciI CCSGG 3 cut(s) 191, 619, 1858
NcoI CCATGG 2 cut(s) 132, 1101
NdeI CATATG 1 cut(s) 525
NlaIV GGNNCC 6 cut(s) 6, 54, 189, 461, 1692, 2168
NmuCI GTSAC 5 cut(s) 239, 581, 665, 2056, 2099
NsiI ATGCAT 2 cut(s) 156, 1641
NspI RCATGY 5 cut(s) 158, 832, 1228, 1360, 1585
PaeI GCATGC 1 cut(s) 158
PaeR7I CTCGAG 1 cut(s) 954
PagI TCATGA 3 cut(s) 24, 1843, 2070
PciI ACATGT 3 cut(s) 828, 1224, 1356
PciSI GCTCTTC 1 cut(s) 1061
PcsI WCGNNNNNNNCGW 1 cut(s) 1546
PctI GAATGC 4 cut(s) 610, 820, 1110, 1641
PdmI GAANNNNTTC 2 cut(s) 1034, 1278
PfeI GAWTC 4 cut(s) 491, 1346, 1758, 2085
PinAI ACCGGT 1 cut(s) 1032
PkrI GCNGC 4 cut(s) 46, 196, 838, 1738
PleI GAGTC 3 cut(s) 655, 1798, 2075
PpsI GAGTC 3 cut(s) 655, 1798, 2075
Ppu21I YACGTR 1 cut(s) 1021
PpuMI RGGWCCY 1 cut(s) 2048
PscI ACATGT 3 cut(s) 828, 1224, 1356
PsiI TTATAA 1 cut(s) 2001
Psp124BI GAGCTC 1 cut(s) 1056
Psp5II RGGWCCY 1 cut(s) 2048
Psp6I CCWGG 5 cut(s) 258, 1298, 1407, 1807, 2050
PspFI CCCAGC 1 cut(s) 752
PspGI CCWGG 5 cut(s) 258, 1298, 1407, 1807, 2050
PspN4I GGNNCC 6 cut(s) 6, 54, 189, 461, 1692, 2168
PspPI GGNCC 3 cut(s) 136, 187, 2048
PspPPI RGGWCCY 1 cut(s) 2048
PstI CTGCAG 1 cut(s) 1933
PstNI CAGNNNCTG 1 cut(s) 2088
PsuI RGATCY 2 cut(s) 1528, 1690
RsaI GTAC 7 cut(s) 226, 251, 530, 623, 717, 1019, 1429
RsaNI GTAC 7 cut(s) 225, 250, 529, 622, 716, 1018, 1428
RseI CAYNNNNRTG 5 cut(s) 29, 468, 1202, 1644, 2194
SacI GAGCTC 1 cut(s) 1056
SapI GCTCTTC 1 cut(s) 1061
SaqAI TTAA 7 cut(s) 168, 416, 918, 1217, 1329, 1554, 1914
SatI GCNGC 4 cut(s) 45, 195, 837, 1737
Sau96I GGNCC 3 cut(s) 136, 187, 2048
ScaI AGTACT 1 cut(s) 717
SchI GAGTC 3 cut(s) 656, 1798, 2076
ScrFI CCNGG 8 cut(s) 191, 260, 619, 1300, 1409, 1809, 1858, 2052
SduI GDGCHC 3 cut(s) 1056, 1129, 1141
SfaNI GCATC 5 cut(s) 191, 739, 963, 1056, 1636
SfcI CTRYAG 3 cut(s) 319, 1380, 1929
Sfr274I CTCGAG 1 cut(s) 954
SinI GGWCC 3 cut(s) 136, 187, 2048
SlaI CTCGAG 1 cut(s) 954
SmiMI CAYNNNNRTG 5 cut(s) 29, 468, 1202, 1644, 2194
SmlI CTYRAG 1 cut(s) 954
SmoI CTYRAG 1 cut(s) 954
SnaBI TACGTA 1 cut(s) 1021
SphI GCATGC 1 cut(s) 158
SsiI CCGC 4 cut(s) 195, 370, 843, 1762
SspMI CTAG 4 cut(s) 803, 1142, 1470, 1682
SstI GAGCTC 1 cut(s) 1056
StyD4I CCNGG 8 cut(s) 189, 258, 617, 1298, 1407, 1807, 1856, 2050
StyI CCWWGG 3 cut(s) 132, 1101, 2124
TaaI ACNGT 4 cut(s) 1340, 1384, 1504, 2009
TaiI ACGT 4 cut(s) 404, 777, 1023, 1952
TaqI TCGA 6 cut(s) 489, 570, 792, 955, 1446, 1540
TatI WGTACW 3 cut(s) 528, 715, 1427
TauI GCSGC 1 cut(s) 197
TfiI GAWTC 4 cut(s) 491, 1346, 1758, 2085
Tru1I TTAA 7 cut(s) 168, 416, 918, 1217, 1329, 1554, 1914
Tru9I TTAA 7 cut(s) 168, 416, 918, 1217, 1329, 1554, 1914
TscAI CASTG 2 cut(s) 586, 2014
TseFI GTSAC 5 cut(s) 239, 581, 665, 2056, 2099
TseI GCWGC 3 cut(s) 44, 836, 1736
Tsp45I GTSAC 5 cut(s) 239, 581, 665, 2056, 2099
TspGWI ACGGA 3 cut(s) 653, 1049, 1347
TspRI CASTG 2 cut(s) 586, 2014
VneI GTGCAC 1 cut(s) 1125
VpaK11BI GGWCC 3 cut(s) 136, 187, 2048
XapI RAATTY 3 cut(s) 233, 273, 1292
XceI RCATGY 5 cut(s) 158, 832, 1228, 1360, 1585
XcmI CCANNNNNNNNNTGG 1 cut(s) 2180
XhoI CTCGAG 1 cut(s) 954
XmiI GTMKAC 1 cut(s) 1097
XmnI GAANNNNTTC 2 cut(s) 1034, 1278
XspI CTAG 4 cut(s) 803, 1142, 1470, 1682
ZrmI AGTACT 1 cut(s) 717
Zsp2I ATGCAT 2 cut(s) 156, 1641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.