Rroxscaffold_1G00005580

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007750
Physical Location & Seq
Reverse (-)
7535969 .. 7537852
1884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00005580.1

Sequence Viewer

Length: 1884 bp
ATGCTTTCAACTCTTTCCCTCCATCTTCCTCCTCCTCCTCTGAACCACAAGTCCCCAACTTCACGAAACCTCACACCCCATCATCATCATCTTCTCCACACCTTCACCTCCCCATTTGAGCTCAAACAACTCCATGCCCACCTCATCAAAACCAACACTCCCCTCTCTTCCCTCCCTCCCACCCGAATCGCCTTCGCTTGCTCTCTCACTCCCAGCTTCTCCTACGCCCGGAAACTCTTCCAACACCTCGAATACCCCGATATCCCTGCCTGGAATTCCTGCCTGAAGGCCTTCGCCGAAGGCGATGAGCCCATTGATGCAATACTGCTCTTCCATCAGCTGCATTGCTTCAATGTAATTCCTGACAGTTTCACTCTGTCTTTTGTTCTAAAGGCCTGTACCCGCTTGTTGGATTTTCACACTGGTAGACTGCTACATGGCTATGTGGAGAAGCTTGGGTTTCAGTCCAATCTGTTTCTGATGAATATGATTCTGAATTTGTATGCATTGTGTGAGGAAATTAGGGATGCACGGCTGATGTTTGATAAAATGCCGCAGAGGGATGTTGTCACGTGGAATATAATGATGACTCTGCTGGTGAAGAGAGGTGATATAGAGCAAGCTTATGATTTGTTTTCGGGGATGCCGGATAGAAGTGTGAGGTCATGGACGCTGATGATTTCGGGGTTTGGTCAGTGCAGTAAGCCTAAGGAGGCGATTCGTGTGTTTTTGGAGATGGAGGAGGCTGGTGTGAGGGCAAATGAGGTGACTGTAGTGGCGGTTCTTGCGGCTTGTGCTGATTTGGGTGACTTGGATTTGGGTAGGAGAGTTCATGAGTACTCGAGCCAAAGTGGGTTTGGAAGAAATGTTTGGATTTGTAACACGCTGATTGAGATGTATGTCAAATGTGGGTGTTTGGAGGATGCTTGTAGAGTTTTTGATGCGATGGAAGAACGAACGGTTGTGTCGTGGTCAGCAATGATTTCAGGGCTGGCCATGCATGGACAAGCTGAGGAAGCTTTGAGACTTTTCTCTAACATGCTTCAGATAGGGATGGAGCCAAATTATGTAACTTTTGTTGGACTCCTGCATGCTTGTAGCCACATGGGGTTTGTGGACAAGGGTCGTGAGTTCTTCGCCAGAATGACTGCAGATTATGGTATAGTTCCGAAAATTGAGCATTATGGTTGTATGGTTGATCTCTTAAGCCGCGCAGGGCTCCTTCAAGAGGCTCACGAGTTTATCATGAACATGCCTATCAAACCAAATGGTGTTGTATGGGGAGCTCTCCTTGGTGGATGCAAAGTTCACAGAAATATTGAACTGGCTGAAGAAGCAACAAAGCACCTTTCTGAACTGGATCCGCTAAATGATGGATACTACATTGTTTTGTCAAACATTTATGCAGAAGCACAACGGTGGGAAGACGTGGCGAGAGTGAGAAGACTAATGAGGGATCGAGGGGTGAAGAAGACACCTGGCTGGAGTTCAATCACGGTAGATGGAGTGCTTCATGAGTTTGTTGCTGGGGATGAAGCCCATCCTGAAGCCGAGGAGATCGACCAAATATGGGAGAAACTACTTGAAAGAATGAAGATGAAGGGTTATGTACCCAACACTTCAGTTGTTCTACTAGACATGGAAGAGGATCAAAAGGAGAAATTTCTCAATCGCCATAGCGAGAAATTAGCGCTGGTTTTTGGGCTGATCAAAACGAAACCTGGAACACCAATTCGAATTATGAAGAATCTTCGTGTTTGTGAGGACTGTCATGCTGCTTTGAAACTTGTATCAGAAATTGCCGACAGAGAGATAGTTGTGCGTGACCGAAATCGGTTCCATTGTTTCAAAAAAGGTTATTGTTCCTGCAGGGATTACTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

627

Amino Acids

71.44

Weight (kDa)

6.39

Isoelectric Point (pI)

44.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 187 - 220 8.7e-08 PPR repeat family
PPR_2 PF13041 219 - 266 9.6e-08 PPR repeat family
PPR PF01535 222 - 251 7e-07 PPR repeat
PPR_2 PF13041 320 - 367 1.4e-10 PPR repeat family
PPR PF01535 322 - 351 7.6e-07 PPR repeat
E_motif PF20431 437 - 498 5.1e-22 E motif
DYW_deaminase PF14432 535 - 627 2.5e-40 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 427
AccII CGCG 1 cut(s) 1212
AciI CCGC 6 cut(s) 403, 554, 779, 788, 1210, 1364
AclWI GGATC 4 cut(s) 1355, 1368, 1464, 1656
AcoI YGGCCR 1 cut(s) 993
AcsI RAATTY 3 cut(s) 274, 496, 1661
AcuI CTGAAG 5 cut(s) 305, 1028, 1350, 1566, 1605
AcvI CACGTG 1 cut(s) 573
AfaI GTAC 3 cut(s) 400, 839, 1611
AfeI AGCGCT 1 cut(s) 1692
AfiI CCNNNNNNNGG 4 cut(s) 228, 409, 1228, 1570
AflII CTTAAG 1 cut(s) 1204
AgsI TTSAA 8 cut(s) 9, 352, 1226, 1322, 1491, 1586, 1783, 1849
AjiI CACGTC 1 cut(s) 1429
AjnI CCWGG 3 cut(s) 269, 1477, 1720
AjuI GAANNNNNNNTTGG 2 cut(s) 853, 885
AleI CACNNNNGTG 1 cut(s) 1417
AloI GAACNNNNNNTCC 4 cut(s) 35, 67, 1290, 1322
AluBI AGCT 8 cut(s) 121, 216, 340, 454, 623, 1010, 1019, 1286
AluI AGCT 8 cut(s) 121, 216, 340, 454, 623, 1010, 1019, 1286
Alw21I GWGCWC 2 cut(s) 123, 1288
Alw26I GTCTC 1 cut(s) 1018
AlwI GGATC 4 cut(s) 1355, 1368, 1464, 1656
Ama87I CYCGRG 1 cut(s) 841
Aor51HI AGCGCT 1 cut(s) 1692
AoxI GGCC 3 cut(s) 288, 393, 993
ApeKI GCWGC 2 cut(s) 340, 1775
ApoI RAATTY 3 cut(s) 274, 496, 1661
Asp700I GAANNNNTTC 2 cut(s) 236, 290
AspLEI GCGC 2 cut(s) 1214, 1693
AsuC2I CCSGG 1 cut(s) 229
AsuHPI GGTGA 6 cut(s) 97, 610, 620, 778, 818, 1477
AsuII TTCGAA 1 cut(s) 1735
AvaI CYCGRG 1 cut(s) 841
AxyI CCTNAGG 1 cut(s) 708
BalI TGGCCA 1 cut(s) 995
BamHI GGATCC 1 cut(s) 1360
BanII GRGCYC 4 cut(s) 123, 312, 1221, 1288
BauI CACGAG 1 cut(s) 1235
BbrPI CACGTG 1 cut(s) 573
BbsI GAAGAC 3 cut(s) 1431, 1450, 1478
Bbv12I GWGCWC 2 cut(s) 123, 1288
BbvCI CCTCAGC 1 cut(s) 1011
BbvI GCAGC 2 cut(s) 327, 1762
BccI CCATC 9 cut(s) 30, 87, 342, 730, 940, 1048, 1367, 1496, 1548
BceAI ACGGC 1 cut(s) 548
BcgI CGANNNNNNTGC 2 cut(s) 248, 282
BciT130I CCWGG 3 cut(s) 271, 1479, 1722
BciVI GTATCC 1 cut(s) 1370
BclI TGATCA 1 cut(s) 1707
BcnI CCSGG 1 cut(s) 229
BcoDI GTCTC 1 cut(s) 1018
BfaI CTAG 1 cut(s) 1634
BfmI CTRYAG 3 cut(s) 771, 1149, 1867
BfoI RGCGCY 1 cut(s) 1694
BfrI CTTAAG 1 cut(s) 1204
BfuI GTATCC 1 cut(s) 1370
BisI GCNGC 5 cut(s) 341, 554, 789, 1210, 1776
BlsI GCNGC 5 cut(s) 342, 555, 790, 1211, 1777
BmcAI AGTACT 1 cut(s) 839
Bme1390I CCNGG 4 cut(s) 229, 271, 1479, 1722
BmeT110I CYCGRG 1 cut(s) 841
BmgBI CACGTC 1 cut(s) 1429
BmiI GGNNCC 4 cut(s) 1059, 1220, 1362, 1838
BmrFI CCNGG 4 cut(s) 229, 271, 1479, 1722
BmsI GCATC 6 cut(s) 307, 517, 633, 913, 931, 1289
BoxI GACNNNNGTC 1 cut(s) 1122
BpiI GAAGAC 3 cut(s) 1431, 1450, 1478
BpmI CTGGAG 1 cut(s) 1504
Bpu10I CCTNAGC 1 cut(s) 1011
Bpu14I TTCGAA 1 cut(s) 1735
BpuMI CCSGG 1 cut(s) 229
BsaAI YACGTR 1 cut(s) 573
BsaJI CCNNGG 2 cut(s) 1291, 1551
BsaXI ACNNNNNCTCC 2 cut(s) 142, 172
Bsc4I CCNNNNNNNGG 4 cut(s) 228, 409, 1228, 1570
Bse1I ACTGG 4 cut(s) 427, 1329, 1362, 1883
Bse21I CCTNAGG 1 cut(s) 708
Bse3DI GCAATG 2 cut(s) 343, 984
BseBI CCWGG 3 cut(s) 271, 1479, 1722
BseDI CCNNGG 2 cut(s) 1291, 1551
BseGI GGATG 8 cut(s) 532, 568, 648, 928, 1059, 1304, 1537, 1540
BseLI CCNNNNNNNGG 4 cut(s) 228, 409, 1228, 1570
BseMI GCAATG 2 cut(s) 343, 984
BseMII CTCAG 1 cut(s) 1002
BseNI ACTGG 4 cut(s) 427, 1329, 1362, 1883
BseRI GAGGAG 5 cut(s) 21, 24, 27, 755, 1568
BseXI GCAGC 2 cut(s) 327, 1762
BseYI CCCAGC 2 cut(s) 212, 1526
BsgI GTGCAG 1 cut(s) 718
Bsh1236I CGCG 1 cut(s) 1212
BshFI GGCC 3 cut(s) 290, 395, 995
BsiHKAI GWGCWC 2 cut(s) 123, 1288
BsiHKCI CYCGRG 1 cut(s) 841
BsiSI CCGG 2 cut(s) 229, 647
BslFI GGGAC 1 cut(s) 37
BslI CCNNNNNNNGG 4 cut(s) 228, 409, 1228, 1570
BsmAI GTCTC 1 cut(s) 1018
BsmFI GGGAC 1 cut(s) 37
BsnI GGCC 3 cut(s) 290, 395, 995
BsoBI CYCGRG 1 cut(s) 841
Bsp119I TTCGAA 1 cut(s) 1735
Bsp1286I GDGCHC 4 cut(s) 123, 312, 1221, 1288
Bsp143I GATC 6 cut(s) 1198, 1360, 1456, 1557, 1648, 1707
BspACI CCGC 6 cut(s) 403, 554, 779, 788, 1210, 1364
BspANI GGCC 3 cut(s) 290, 395, 995
BspCNI CTCAG 1 cut(s) 1003
BspFNI CGCG 1 cut(s) 1212
BspHI TCATGA 3 cut(s) 832, 1245, 1513
BspLI GGNNCC 4 cut(s) 1059, 1220, 1362, 1838
BspMAI CTGCAG 2 cut(s) 1153, 1871
BspPI GGATC 4 cut(s) 1355, 1368, 1464, 1656
BspQI GCTCTTC 1 cut(s) 335
BspT104I TTCGAA 1 cut(s) 1735
BspTI CTTAAG 1 cut(s) 1204
BsrDI GCAATG 2 cut(s) 343, 984
BsrI ACTGG 4 cut(s) 427, 1329, 1362, 1883
BssECI CCNNGG 2 cut(s) 1291, 1551
BssMI GATC 6 cut(s) 1198, 1360, 1456, 1557, 1648, 1707
BssSI CACGAG 1 cut(s) 1235
BssT1I CCWWGG 1 cut(s) 1291
Bst2BI CACGAG 1 cut(s) 1235
Bst2UI CCWGG 3 cut(s) 271, 1479, 1722
Bst4CI ACNGT 6 cut(s) 368, 772, 961, 1419, 1498, 1769
Bst6I CTCTTC 5 cut(s) 172, 242, 335, 596, 1638
BstAFI CTTAAG 1 cut(s) 1204
BstBAI YACGTR 1 cut(s) 573
BstBI TTCGAA 1 cut(s) 1735
BstC8I GCNNGC 4 cut(s) 199, 621, 993, 1092
BstDEI CTNAG 2 cut(s) 708, 1011
BstENI CCTNNNNNAGG 1 cut(s) 1226
BstF5I GGATG 8 cut(s) 532, 568, 648, 928, 1059, 1304, 1537, 1540
BstFNI CGCG 1 cut(s) 1212
BstH2I RGCGCY 1 cut(s) 1694
BstHHI GCGC 2 cut(s) 1214, 1693
BstKTI GATC 6 cut(s) 1201, 1363, 1459, 1560, 1651, 1710
BstMAI GTCTC 1 cut(s) 1018
BstMBI GATC 6 cut(s) 1198, 1360, 1456, 1557, 1648, 1707
BstMWI GCNNNNNNNGC 5 cut(s) 785, 794, 997, 1016, 1334
BstNI CCWGG 3 cut(s) 271, 1479, 1722
BstNSI RCATGY 3 cut(s) 1042, 1094, 1255
BstPAI GACNNNNGTC 1 cut(s) 1122
BstSCI CCNGG 4 cut(s) 227, 269, 1477, 1720
BstSFI CTRYAG 3 cut(s) 771, 1149, 1867
BstUI CGCG 1 cut(s) 1212
BstV1I GCAGC 2 cut(s) 327, 1762
BstV2I GAAGAC 3 cut(s) 1431, 1450, 1478
BstX2I RGATCY 1 cut(s) 1360
BstYI RGATCY 1 cut(s) 1360
Bsu36I CCTNAGG 1 cut(s) 708
BsuI GTATCC 1 cut(s) 1370
BsuRI GGCC 3 cut(s) 290, 395, 995
BtgZI GCGATG 2 cut(s) 318, 959
BtrI CACGTC 1 cut(s) 1429
BtsCI GGATG 8 cut(s) 532, 568, 648, 928, 1059, 1304, 1537, 1540
BtsIMutI CAGTG 2 cut(s) 420, 701
Cac8I GCNNGC 4 cut(s) 199, 621, 993, 1092
CciI TCATGA 3 cut(s) 832, 1245, 1513
CfoI GCGC 2 cut(s) 1214, 1693
CseI GACGC 1 cut(s) 679
Csp6I GTAC 3 cut(s) 399, 838, 1610
CviQI GTAC 3 cut(s) 399, 838, 1610
DdeI CTNAG 2 cut(s) 708, 1011
DpnI GATC 6 cut(s) 1200, 1362, 1458, 1559, 1650, 1709
DpnII GATC 6 cut(s) 1198, 1360, 1456, 1557, 1648, 1707
EaeI YGGCCR 1 cut(s) 993
Eam1104I CTCTTC 5 cut(s) 172, 242, 335, 596, 1638
EarI CTCTTC 5 cut(s) 172, 242, 335, 596, 1638
Ecl136II GAGCTC 2 cut(s) 121, 1286
Eco130I CCWWGG 1 cut(s) 1291
Eco147I AGGCCT 2 cut(s) 290, 395
Eco24I GRGCYC 4 cut(s) 123, 312, 1221, 1288
Eco32I GATATC 1 cut(s) 262
Eco47III AGCGCT 1 cut(s) 1692
Eco53kI GAGCTC 2 cut(s) 121, 1286
Eco57I CTGAAG 5 cut(s) 305, 1028, 1350, 1566, 1605
Eco72I CACGTG 1 cut(s) 573
Eco81I CCTNAGG 1 cut(s) 708
Eco88I CYCGRG 1 cut(s) 841
EcoICRI GAGCTC 2 cut(s) 121, 1286
EcoNI CCTNNNNNAGG 1 cut(s) 1226
EcoRI GAATTC 1 cut(s) 274
EcoRII CCWGG 3 cut(s) 269, 1477, 1720
EcoRV GATATC 1 cut(s) 262
EcoT14I CCWWGG 1 cut(s) 1291
EcoT22I ATGCAT 2 cut(s) 508, 1002
EcoT38I GRGCYC 4 cut(s) 123, 312, 1221, 1288
ErhI CCWWGG 1 cut(s) 1291
FaqI GGGAC 1 cut(s) 37
FauI CCCGC 1 cut(s) 410
FbaI TGATCA 1 cut(s) 1707
FblI GTMKAC 1 cut(s) 427
Fnu4HI GCNGC 5 cut(s) 341, 554, 789, 1210, 1776
FokI GGATG 8 cut(s) 539, 575, 655, 935, 1066, 1311, 1527, 1544
FriOI GRGCYC 4 cut(s) 123, 312, 1221, 1288
Fsp4HI GCNGC 5 cut(s) 341, 554, 789, 1210, 1776
FspBI CTAG 1 cut(s) 1634
GlaI GCGC 2 cut(s) 1213, 1692
GluI GCNGC 5 cut(s) 341, 554, 789, 1210, 1776
GsaI CCCAGC 2 cut(s) 216, 1530
GsuI CTGGAG 1 cut(s) 1504
HaeII RGCGCY 1 cut(s) 1694
HaeIII GGCC 3 cut(s) 290, 395, 995
HapII CCGG 2 cut(s) 229, 647
HgaI GACGC 1 cut(s) 679
HhaI GCGC 2 cut(s) 1214, 1693
Hin6I GCGC 2 cut(s) 1212, 1691
HinP1I GCGC 2 cut(s) 1212, 1691
HindIII AAGCTT 3 cut(s) 452, 621, 1017
HinfI GANTC 6 cut(s) 186, 490, 589, 718, 1083, 1747
HpaII CCGG 2 cut(s) 229, 647
HphI GGTGA 6 cut(s) 97, 610, 620, 778, 818, 1477
Hpy166II GTNNAC 3 cut(s) 428, 1117, 1309
Hpy188I TCNGA 7 cut(s) 42, 480, 495, 1047, 1170, 1354, 1795
Hpy188III TCNNGA 9 cut(s) 63, 362, 833, 1127, 1226, 1235, 1246, 1514, 1544
Hpy8I GTNNAC 3 cut(s) 428, 1117, 1309
HpyAV CCTTC 7 cut(s) 112, 202, 280, 293, 301, 1232, 1594
HpyCH4III ACNGT 6 cut(s) 368, 772, 961, 1419, 1498, 1769
HpyCH4IV ACGT 2 cut(s) 572, 1428
HpyF10VI GCNNNNNNNGC 5 cut(s) 785, 794, 997, 1016, 1334
HpyF3I CTNAG 2 cut(s) 708, 1011
HpySE526I ACGT 2 cut(s) 572, 1428
HspAI GCGC 2 cut(s) 1212, 1691
Ksp22I TGATCA 1 cut(s) 1707
Kzo9I GATC 6 cut(s) 1198, 1360, 1456, 1557, 1648, 1707
LguI GCTCTTC 1 cut(s) 335
LmnI GCTCC 3 cut(s) 1057, 1224, 1283
Lsp1109I GCAGC 2 cut(s) 327, 1762
LweI GCATC 6 cut(s) 307, 517, 633, 913, 931, 1289
MaeI CTAG 1 cut(s) 1634
MaeII ACGT 2 cut(s) 572, 1428
MaeIII GTNAC 6 cut(s) 568, 766, 806, 878, 1069, 1823
MalI GATC 6 cut(s) 1200, 1362, 1458, 1559, 1650, 1709
MboI GATC 6 cut(s) 1198, 1360, 1456, 1557, 1648, 1707
MflI RGATCY 1 cut(s) 1360
MhlI GDGCHC 4 cut(s) 123, 312, 1221, 1288
MlsI TGGCCA 1 cut(s) 995
MluNI TGGCCA 1 cut(s) 995
MlyI GAGTC 2 cut(s) 583, 1077
MmeI TCCRAC 3 cut(s) 265, 390, 1060
Mox20I TGGCCA 1 cut(s) 995
Mph1103I ATGCAT 2 cut(s) 508, 1002
MroXI GAANNNNTTC 2 cut(s) 236, 290
MscI TGGCCA 1 cut(s) 995
MseI TTAA 1 cut(s) 1205
MslI CAYNNNNRTG 3 cut(s) 441, 1250, 1417
Msp20I TGGCCA 1 cut(s) 995
MspA1I CMGCKG 1 cut(s) 340
MspCI CTTAAG 1 cut(s) 1204
MspI CCGG 2 cut(s) 229, 647
MspR9I CCNGG 4 cut(s) 229, 271, 1479, 1722
MvaI CCWGG 3 cut(s) 271, 1479, 1722
MvnI CGCG 1 cut(s) 1212
MwoI GCNNNNNNNGC 5 cut(s) 785, 794, 997, 1016, 1334
NciI CCSGG 1 cut(s) 229
NdeII GATC 6 cut(s) 1198, 1360, 1456, 1557, 1648, 1707
NlaIV GGNNCC 4 cut(s) 1059, 1220, 1362, 1838
NmeAIII GCCGAG 1 cut(s) 1576
NmuCI GTSAC 4 cut(s) 568, 766, 806, 1823
NsiI ATGCAT 2 cut(s) 508, 1002
NspI RCATGY 3 cut(s) 1042, 1094, 1255
NspV TTCGAA 1 cut(s) 1735
OliI CACNNNNGTG 1 cut(s) 1417
PaeI GCATGC 1 cut(s) 1094
PaeR7I CTCGAG 1 cut(s) 841
PagI TCATGA 3 cut(s) 832, 1245, 1513
PceI AGGCCT 2 cut(s) 290, 395
PciSI GCTCTTC 1 cut(s) 335
PcsI WCGNNNNNNNCGW 3 cut(s) 255, 300, 965
PdmI GAANNNNTTC 2 cut(s) 236, 290
PfeI GAWTC 4 cut(s) 186, 490, 718, 1747
PkrI GCNGC 5 cut(s) 342, 555, 790, 1211, 1777
PleI GAGTC 2 cut(s) 583, 1077
PmaCI CACGTG 1 cut(s) 573
PmlI CACGTG 1 cut(s) 573
PpsI GAGTC 2 cut(s) 583, 1077
Ppu21I YACGTR 1 cut(s) 573
PshAI GACNNNNGTC 1 cut(s) 1122
Psp124BI GAGCTC 2 cut(s) 123, 1288
Psp6I CCWGG 3 cut(s) 269, 1477, 1720
PspCI CACGTG 1 cut(s) 573
PspFI CCCAGC 2 cut(s) 212, 1526
PspGI CCWGG 3 cut(s) 269, 1477, 1720
PspN4I GGNNCC 4 cut(s) 1059, 1220, 1362, 1838
PspXI VCTCGAGB 1 cut(s) 841
PsrI GAACNNNNNNTAC 2 cut(s) 765, 797
PstI CTGCAG 2 cut(s) 1153, 1871
PsuI RGATCY 1 cut(s) 1360
PvuII CAGCTG 1 cut(s) 340
RsaI GTAC 3 cut(s) 400, 839, 1611
RsaNI GTAC 3 cut(s) 399, 838, 1610
RseI CAYNNNNRTG 3 cut(s) 441, 1250, 1417
SacI GAGCTC 2 cut(s) 123, 1288
SapI GCTCTTC 1 cut(s) 335
SaqAI TTAA 1 cut(s) 1205
SatI GCNGC 5 cut(s) 341, 554, 789, 1210, 1776
Sau3AI GATC 6 cut(s) 1198, 1360, 1456, 1557, 1648, 1707
SbfI CCTGCAGG 1 cut(s) 1871
ScaI AGTACT 1 cut(s) 839
SchI GAGTC 2 cut(s) 583, 1077
ScrFI CCNGG 4 cut(s) 229, 271, 1479, 1722
SdaI CCTGCAGG 1 cut(s) 1871
SduI GDGCHC 4 cut(s) 123, 312, 1221, 1288
SfaNI GCATC 6 cut(s) 307, 517, 633, 913, 931, 1289
SfcI CTRYAG 3 cut(s) 771, 1149, 1867
Sfr274I CTCGAG 1 cut(s) 841
SfuI TTCGAA 1 cut(s) 1735
SlaI CTCGAG 1 cut(s) 841
SmiMI CAYNNNNRTG 3 cut(s) 441, 1250, 1417
SmlI CTYRAG 2 cut(s) 841, 1204
SmoI CTYRAG 2 cut(s) 841, 1204
SphI GCATGC 1 cut(s) 1094
Sse8387I CCTGCAGG 1 cut(s) 1871
SseBI AGGCCT 2 cut(s) 290, 395
SsiI CCGC 6 cut(s) 403, 554, 779, 788, 1210, 1364
SspI AATATT 1 cut(s) 1318
SspMI CTAG 1 cut(s) 1634
SstI GAGCTC 2 cut(s) 123, 1288
StuI AGGCCT 2 cut(s) 290, 395
StyD4I CCNGG 4 cut(s) 227, 269, 1477, 1720
StyI CCWWGG 1 cut(s) 1291
TaaI ACNGT 6 cut(s) 368, 772, 961, 1419, 1498, 1769
TaiI ACGT 2 cut(s) 575, 1431
TaqI TCGA 5 cut(s) 249, 842, 1459, 1560, 1735
TaqII GACCGA 1 cut(s) 1842
TatI WGTACW 1 cut(s) 837
TauI GCSGC 3 cut(s) 556, 791, 1212
TfiI GAWTC 4 cut(s) 186, 490, 718, 1747
Tru1I TTAA 1 cut(s) 1205
Tru9I TTAA 1 cut(s) 1205
TscAI CASTG 2 cut(s) 427, 701
TseFI GTSAC 4 cut(s) 568, 766, 806, 1823
TseI GCWGC 2 cut(s) 340, 1775
Tsp45I GTSAC 4 cut(s) 568, 766, 806, 1823
TspDTI ATGAA 8 cut(s) 497, 821, 1262, 1502, 1548, 1607, 1613, 1757
TspRI CASTG 2 cut(s) 427, 701
Vha464I CTTAAG 1 cut(s) 1204
XagI CCTNNNNNAGG 1 cut(s) 1226
XapI RAATTY 3 cut(s) 274, 496, 1661
XceI RCATGY 3 cut(s) 1042, 1094, 1255
XcmI CCANNNNNNNNNTGG 1 cut(s) 854
XhoI CTCGAG 1 cut(s) 841
XmiI GTMKAC 1 cut(s) 427
XmnI GAANNNNTTC 2 cut(s) 236, 290
XspI CTAG 1 cut(s) 1634
ZrmI AGTACT 1 cut(s) 839
Zsp2I ATGCAT 2 cut(s) 508, 1002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.