FvH4_3g45741

Belongs to the peptidase C2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007783
Physical Location & Seq
Reverse (-)
37942791 .. 37958527
15737 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g45741.t1

Sequence Viewer

Length: 5958 bp
ATGCTCTTTATCTGTAGAATGGTGTTCAATGGAAATGGCTTAGATGTAGATGAGTATGTGAGGAAGGCATACAAATTTGCTTATTCTGATTGTATAGAAGTGGGTCCTGTGGCTTGTTTACCAGAACCACCAGATCCAAATGAGTTATATCCTCGACAATCTAGCAGGGCTTCACATCTTGGGCTTCTTTACCTTGGCTCACTTGTAGTTCTTCTTGTGTACTCAATCCTGTATGGTCTTACTGCAAAGGACTCACGTTGGCTTGGAGCCATTACATCAGCTGCTGTGATAATTCTTGATTGGAACATGGGAGCGTGTTTATATGGGTTTGAACTCCTTAACAGTCGTGTTGCAGCGTTATTTGTTGCTGGAACATCTCGAATATTTCTCATTTGCTTCGGCGTTCACTACTGGTACTTGGGGCACTGCATTAGTTATGCAGTTGTAGCATCTGTTTTATTAGGTGCTTCTGTTTCTCGTCATTTATCTGTAACAAACCCATTGGCTGCAAGGAGAGATGCCTTACAGAGTACTGTGATACGACTGAGAGAGGGTTTCCGCAAAAAAGAACATAACAGTTCATCAAGCTCGTCTGAAGGTTGTGGCTCAAGCATGAAACGTAGTGGTAGTGTTGAAGCAGGTTGCCTCGGTAATGTTGTTGAAGCAAGTAACAGGAGCACAACACAGAGCACAGTTGATGCTAATAACTGGAGTAATGTTCTATTACGAACTGCGAGTTCTCATGAGGGGATTAATAGTGATAAAAGCATAGATAGTGGGAGGCCTAGTATAGCATTGTGTAGCAGCTCCTGTCGTTCAGTAATCCAAGAGCCGGAAGTGGGAACGTCCTTTACTGACAAAAATTGCGATCAAAGTAGCACCTTGGTGGTTTGTTCTAGCAGTGGTCTTGAAAGCCAAGGCTGTGAATCCAGTGCATCAAATTCTGCAAATCAACAAACTTTGGATTTGAATTTAGCTTTTGCTTTGCAAGAAAGGTTGAATGACCCCAGGATAACATCTATGCTAAAGAAGAGGGGAAGACAAGGTGACCTTGAACTAGTTAATCTTTTGCAGGATAAAGGATTGGATCCTAACTTTGCTATGATGTTGAAGGAGAAGAGCTTGGATCCAACCATTCTGGCATTACTTCAAAGAAGCAGTTTGGATGCTGATAGAGATCATCGTGACAATACAGATATCACCATAGCTGACTCAAATAGTGTGGATAATGGTTTACCCAATCAAATATCTTTATCAGAAGAACTGAGGCTTCATGGGCTTGAAAAATGGCTCCAATTATCTAGGCTTGTTTTGCACCATGTAGTAGGTACTCCAGAGAGAGCATGGGTTCTCTTTAGTTTTGTTTTCATCCTTGAAACAATTGCTGTGGCAATTGTCCGTCCAAAGATAATCAAAATAATAAATGCGACCCATCAACAGTTTGAATTTGGGTTTGCGGTGCTGCTGTTATCTCCTGTCGTCTGTTCAATCATGGCTTTTCTTCGATCGCTTCAAGCAGAGGAAATGGTCATGACCTCTAAGCCGCGCAAGTATGGTTTTGTTGCTTGGCTGCTGAGCACCTGTGTTGGATTATTGCTTTCCTTCTTGAGCAAATCATCAGTTCTTCTGGGACTGTCATTGACTGTTCCCGTCATGGTGGCATGCCTTTCTGTTGCCATTCCAACATGGAACCGTAATGGCTACCAATTTTGGGTTCCTCAATTACATTGTGCAGGTTCTGCAGGGAACCAACAAATTCGGGGAACAAAAGAGGGTGTTATTCTTGTCTTCTGCACAACATTATTTGCTGGATCTGTACTAGCTCTTGGCACAATAGTGTCTGCAAAGCCCTTGGATGACTTGGGATACAAAGGATGGACGGGTGAACAGAAAAGCTTTACATCTCCTTATGCATCATCTGTGTACATTGGCTGGGCTATGGCTTCTGCGATTGCTTTAGTGGTTACTGGTGTACTGCCGATTGTTTCATGGTTTGCATCCTACCGGTTCTCCCATTTTTCTGCTGTGTGTGTCGGGATATTTACGGCTGTCCTTGTGTCATTTTGCGGTGCATCTTACATCGAGGTTGTGAAATCTAGAGATGACCAAGTTCCTACAAAGGGTGACTTCCTTGCTGCTTTACTTCCTTTAATTTGCATTCCAGCATTTCTGTCTCTGTGCTCTGGGCTGTATAAATGGAAGGATGATAATTGGAAGCTTTCTCGGGGTGTGTATATCTTTGTAACCATAGGTCTTCTTCTTCTGCTCGGAGCAATATCAGCTGTTATAGTCGTAGTTACACCATGGACGATTGGGGTATCATTTCTTTTAGTTCTTCTCATGATTGTACTGGCTATTGGTGCTATACATCATTGGGCCTCAAACAACTTTTATTTGACAAGGACCCAGACATTCTTTGTTTGTTTCCTCGCTTTTCTTTTGGCACTGGCTGCACTTCTTGTTGGATGGTTTGAAGATAAACCATTTGTCGGTGCTTCTGTTGGCTACTTTTTGTTTTTGTTTCTTCTTGCCGGGAGAGCATTGACTGTTCTTCTTTCACCTCCTATTGTTGTTTATTCTCCAAGAGTTCTCCCTGTTTATGTTTATGATGCTCATGCAGATTGTGCAAAGAATGTCAGTGCTGCATTTCTTGTGCTTTATGGGATTGCATTGGCAACTGAGGGCTGGGGTGTTGTTGCGAGTTTGAAAATTTATCCACCATTTGCTGGTGCCGCTGTATCAGCAATTACTCTTGTTGTATCCTTTGGGTTTGCTTTCTCTCGTCCCTGCTTGACCCTCAAGATGATGGAAGATGCCGTACACTTTCTTAGTAAAGAAACCGTTGTTCAAGCGATTGCTAGATCTGCCACCAAGACCAGAAATGCTTTATCTGGAACTTATTCGGCTCCACAGAGGTCTGCCAGTTCAGCTGCTCTTTTAGTTGGAGATCCCACGATAATGCGTGATAGGGCAGGAAATTTTGTGCTTCCCAGAGCAGATGTCATGAAATTGAGAGATCGCTTAAGAAATGAGGAGCTAGTTGCAGGATCATTTTTCGGAAGAATGAGATATGGAAGGACATTCCGCCATGAGCCACCTAGTAGTATCGATCACAGAAGAGAGATGTGTGCTCATGCACGAATATTGGCTTTGGAAGAGGCGATTGATACTGAATGGGTATATATGTGGGATAAATTTGGTGGTTATCTGCTTCTTTTGCTTGGTTTGACTGCTAAGGCAGAGCGAGTACAGGATGAGGTACGCTTGAGACTCTTTCTTGATAGCATAGGATTTGCAGACTTAAGTGCCAAGAAAATAAAAAAGTGGATGCCAGAGGACCGTAGGCAGTTTGAGATTATTCAGGAGAGTTACCTAAGAGAAAAGGAGATGGAAGAGGAACTCTTGATGCAAAGACGTGAAGAGGAGGGGAAAGGCAAAGAGAGAAGAAAGGCTCTCTTGGAGAAGGAAGAACGCAAATGGAAGGAGATAGAAGCGTCTTTAATTTCTTCTATTCCTAATGCTGGCAGCAGGGAAGCTGCAGCTGTGGCAGCTGCAGTGCGTGCAGTAGGGGGTGATTCAGTTCTTGATGATTCTTTTGCTAGAGAGAGAGTTTCAAGCATTGCGCGTCGGATACGCACAGCTCAGCTAACTCGGCGAGCACTTCAGACTGGAATTTCGGGTGCTGTATGCGTTCTAGACGATGAACCGACAACAAGTGGCAGGCACTGTGGCCAGATTGAATCCAGCATCTGTCAAAGTCAGAAGATCAGCTTTTCCATTGCAGTGATGATTCAACCCGTGTCTGGGCCTGTTTGCCTATTAGGCACTGAGTTTCAAAAGAAAATTTGCTGGGAAATATTGGTTGCCGGTTCCGAGCAAGGAATTGAAGCTGGACAAGTTGGGCTTAGATTGATCACTAAAGGCGACAGACAAACTACTGTTGCAAAGGAATGGAGTATTGGTGCAACTAGTATTGCAGATGGAAGGTGGCATTTGGTAACAATGACAATTGATGCTGATTTAGGTGAGGCCACTTGCTATCTCGATGGTGGTTTTGATGGCTACCAGACCGGTTTACCATTGCATGTGGGCAATACTATTTGGGAGCTAGGAACCGAAGTTTGGGTAGGTGTTAGACCGCCTACAGATATGGATGCATTTGGGAGGTCAGATAGTGAAGGGGCTGAGTCTAAGATGCATATTATGGATGTGTTTTTATGGGGAAGGTGCTTAACGGAGGATGACATTGCTGCTCTCCATGCAGCTGTTGGGTCAGCTGACACTAGTATGATAGATTTTCCTGAAGATGCTTGGCAGTGGGCAGATTCACCTTCTAGAGTTGATGAATGGGATAGTGATCATGCCGAAGTAGAACTATATGATAGGGATGAAGTTGATTCGGATGGGCAATATTCAAGTGGGAGGAAAAGAAGGTCAGAACGTGATGGAGTTCTGGTTGATATGGATTCTTTTGCCAGGAGATTTAGGAAACCTAGGATGGAAACACAAGAAGAAATAAATCAACGTATGCTTTCTGTTGAATTGGCTGTCAAAGAAGCTCTCTGTGCAAGAGGAGAAACAAATTTTACTGATCAGGAGTTTCCTCCAAATGATCAATCATTATTTGTAGATTCAGAAAATCCCCCCTCAAAGTTGCAGGTCGTTTCAGAATGGATGAGGCCTGCTGATATTGTAAAAGAGAGCAGGCTTGGTGCTCGGCCCTGCTTATTTTCTGGCACTGTGAATCCTTCTGATGTTTGTCAGGGTCGACTGGGTGATTGTTGGTTTTTGAGTGCTGTTGCTGTTCTGACGGAAGTTTCTCGAATATCGGAAGTTATTATTACTCCAGAATATAACGAGGAAGGAATCTACACTGTCCGCTTCTGTATTCAGGGTGAGTGGGTTCCTGTTGTTGTTGATGATTGGATTCCATGCGAATCACCGGGTAAACCAGCATTTGCTACTAGCAGAAAAGGTAACGAGTTGTGGGTCTCTTTACTGGAGAAGGCATATGCCAAATTGCATGGCTCCTATGAAGCTTTAGAAGGTGGACTTGTCCAAGATGCTCTTGTAGACCTCACTGGAGGTGCGGGGGAAGAGATTGACATGAGGAGTGCTCAGGCCCAAATTGATCTTGCGAGTGGTCGATTATGGTCTCAGTTGTTGCGCTTCAAACAGGAGGGATTTCTTCTGGGTGCTGGAAGTCCATCTGGTTCAGATGTACATATTTCCTCCAGTGGCATTGTCCAAGGTCATGCTTACTCATTATTGCAGGTGAGAGAGGTGGATGGTCACAAGCTTATTCAGATTCGGAATCCATGGGCCAATGAAGTCGAGTGGAATGGCCCTTGGTCTGACTCATCGCCTGAATGGACTGATAGGATGAAGCACAAGCTGAAGCATATTCCACAGTCAAAAGATGGCATATTCTGGATGTCCTGGCAAGACTTCCAAATTCATTTTCGATCAATATATGTTTGCCGTATCTATCCCCCAGAGATGCGCTACTCTGTTCACGGCCAATGGCGAAGTTATAGTGCTGGTGGTTGCCAAGACTATGAGACATGGCATCAAAATCCACAATTCCGATTGCGGGCCACAGGGCCAGATGCCTCATTTCCAATTCATGTATTCATTACATTGACACAGGGTGTGAGCTTCTCAAGAACAGTTGCTGGTTTCAGAAACTATCAATCCAGCCATGATTCAATGATGTTCTATATTGGGATGAGGATACTCAAAACCCGAGGCCGCCGAGCTGCTTACAATATATACCTACATGAATCAGTTGGTGGGACAGATTATGTTAATTCACGAGAAATATCGTGTGAAATGGTTTTGGATCCAGATCCAAAAGGTTACACAATAGTCCCAACAACTATACACCCTGGCGAAGAAGCTCCATTTGTTCTTTCTGTTTTTACAAAAGCATCAATAACCTTGGAAGCCTTGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001558 GO:0001708 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0004198 GO:0005198 GO:0005488 GO:0005509 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0005829 GO:0005886 GO:0005887 GO:0006275 GO:0006355 GO:0006508 GO:0006807 GO:0006873 GO:0006874 GO:0006875 GO:0006950 GO:0006970 GO:0006996 GO:0007010 GO:0007204 GO:0007275 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0008307 GO:0009628 GO:0009651 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009934 GO:0009966 GO:0009987 GO:0010008 GO:0010035 GO:0010038 GO:0010154 GO:0010468 GO:0010522 GO:0010524 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010646 GO:0010927 GO:0010941 GO:0010959 GO:0012505 GO:0014717 GO:0014718 GO:0014823 GO:0014850 GO:0016020 GO:0016021 GO:0016043 GO:0016787 GO:0019219 GO:0019222 GO:0019538 GO:0019725 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0030003 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030036 GO:0030154 GO:0030162 GO:0030234 GO:0030239 GO:0030315 GO:0031032 GO:0031090 GO:0031224 GO:0031226 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031402 GO:0031410 GO:0031420 GO:0031432 GO:0031674 GO:0031982 GO:0031984 GO:0032101 GO:0032268 GO:0032269 GO:0032270 GO:0032501 GO:0032502 GO:0032875 GO:0032877 GO:0032879 GO:0032947 GO:0032989 GO:0032991 GO:0033036 GO:0033233 GO:0033234 GO:0033554 GO:0034613 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0040008 GO:0042127 GO:0042175 GO:0042221 GO:0042383 GO:0042592 GO:0042692 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043269 GO:0043270 GO:0043292 GO:0043415 GO:0043416 GO:0043933 GO:0044085 GO:0044093 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044449 GO:0044459 GO:0044464 GO:0045165 GO:0045214 GO:0045740 GO:0045787 GO:0045862 GO:0045892 GO:0045893 GO:0045927 GO:0045934 GO:0045935 GO:0046872 GO:0048316 GO:0048468 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048583 GO:0048584 GO:0048608 GO:0048638 GO:0048639 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050801 GO:0050865 GO:0050867 GO:0050896 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051146 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051239 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051279 GO:0051281 GO:0051282 GO:0051480 GO:0051592 GO:0051641 GO:0051716 GO:0051726 GO:0051924 GO:0051928 GO:0055001 GO:0055002 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055103 GO:0060255 GO:0060341 GO:0060548 GO:0061041 GO:0061061 GO:0061458 GO:0065003 GO:0065007 GO:0065008 GO:0065009 GO:0070011 GO:0070727 GO:0070887 GO:0070925 GO:0071214 GO:0071241 GO:0071248 GO:0071277 GO:0071470 GO:0071472 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072657 GO:0080050 GO:0080090 GO:0080134 GO:0090068 GO:0090303 GO:0090329 GO:0090392 GO:0090627 GO:0090628 GO:0097264 GO:0097435 GO:0097708 GO:0098588 GO:0098771 GO:0098772 GO:0098805 GO:0098827 GO:0099080 GO:0099081 GO:0099512 GO:0104004 GO:0140096 GO:1901564 GO:1902531 GO:1902679 GO:1902680 GO:1903034 GO:1903036 GO:1903169 GO:1903320 GO:1903321 GO:1903506 GO:1903507 GO:1903508 GO:1904062 GO:1904064 GO:1904427 GO:2000011 GO:2000014 GO:2000024 GO:2000026 GO:2000105 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calpain_III Peptidase_C2

Protein Analysis

1986

Amino Acids

219.45

Weight (kDa)

5.96

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C2 PF00648 1529 - 1828 4.3e-89 Calpain family cysteine protease
Calpain_III PF01067 1839 - 1974 9.9e-16 Calpain large subunit, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 5263
Acc36I ACCTGC 4 cut(s) 629, 1724, 4648, 5263
AccB1I GGYRCC 1 cut(s) 2729
AccB7I CCANNNNNTGG 1 cut(s) 2726
AccI GTMKAC 2 cut(s) 4768, 5073
AccII CGCG 2 cut(s) 1546, 3625
AcoI YGGCCR 2 cut(s) 3730, 5518
AcuI CTGAAG 4 cut(s) 615, 3647, 4323, 5417
AdeI CACNNNGTG 1 cut(s) 5651
AflII CTTAAG 2 cut(s) 3022, 3301
AgeI ACCGGT 2 cut(s) 2004, 4070
AhlI ACTAGT 3 cut(s) 1057, 3968, 4283
AjiI CACGTC 1 cut(s) 3416
AjnI CCWGG 4 cut(s) 1007, 4475, 5438, 5887
AjuI GAANNNNNNNTTGG 8 cut(s) 1698, 1730, 3942, 3974, 4105, 4137, 5445, 5477
AleI CACNNNNGTG 2 cut(s) 884, 1835
Alw21I GWGCWC 8 cut(s) 680, 692, 1580, 2183, 3133, 3661, 4717, 5119
Alw26I GTCTC 5 cut(s) 2178, 3262, 4996, 5160, 5555
AlwNI CAGNNNCTG 6 cut(s) 284, 810, 1739, 3726, 3750, 5675
Ama87I CYCGRG 2 cut(s) 2223, 5745
ArsI GACNNNNNNTTYG 2 cut(s) 3658, 3690
AseI ATTAAT 1 cut(s) 753
AsiGI ACCGGT 2 cut(s) 2004, 4070
Asp700I GAANNNNTTC 1 cut(s) 2899
AspA2I CCTAGG 1 cut(s) 4493
AspLEI GCGC 4 cut(s) 1548, 3625, 5169, 5505
AsuC2I CCSGG 2 cut(s) 2533, 4944
AvaI CYCGRG 2 cut(s) 2223, 5745
AvaII GGWCC 3 cut(s) 104, 2403, 3337
AvrII CCTAGG 1 cut(s) 4493
BaeGI GKGCMC 1 cut(s) 426
BalI TGGCCA 1 cut(s) 3732
BamHI GGATCC 3 cut(s) 1087, 1126, 5842
BanI GGYRCC 1 cut(s) 2729
BarI GAAGNNNNNNTAC 2 cut(s) 2802, 2834
BauI CACGAG 1 cut(s) 5814
BbsI GAAGAC 3 cut(s) 1045, 1780, 2246
Bbv12I GWGCWC 8 cut(s) 680, 692, 1580, 2183, 3133, 3661, 4717, 5119
BceAI ACGGC 4 cut(s) 2061, 2801, 5466, 5533
BciT130I CCWGG 4 cut(s) 1009, 4477, 5440, 5889
BciVI GTATCC 4 cut(s) 1859, 2770, 3624, 5727
BclI TGATCA 4 cut(s) 3912, 4357, 4591, 4612
BcnI CCSGG 2 cut(s) 2533, 4944
BcoDI GTCTC 5 cut(s) 2178, 3262, 4996, 5160, 5555
BcuI ACTAGT 3 cut(s) 1057, 3968, 4283
BfmI CTRYAG 5 cut(s) 13, 1740, 3537, 3552, 4143
BfrI CTTAAG 2 cut(s) 3022, 3301
BfuAI ACCTGC 4 cut(s) 629, 1724, 4648, 5263
BfuI GTATCC 4 cut(s) 1859, 2770, 3624, 5727
BglI GCCNNNNNGGC 1 cut(s) 3822
BglII AGATCT 1 cut(s) 2861
BlnI CCTAGG 1 cut(s) 4493
BlpI GCTNAGC 2 cut(s) 1574, 3642
BmcAI AGTACT 1 cut(s) 532
Bme1390I CCNGG 6 cut(s) 1009, 2533, 4477, 4944, 5440, 5889
Bme18I GGWCC 3 cut(s) 104, 2403, 3337
BmeT110I CYCGRG 2 cut(s) 2223, 5745
BmgBI CACGTC 1 cut(s) 3416
BmrFI CCNGG 6 cut(s) 1009, 2533, 4477, 4944, 5440, 5889
BmrI ACTGGG 1 cut(s) 4781
BmuI ACTGGG 1 cut(s) 4781
BpiI GAAGAC 3 cut(s) 1045, 1780, 2246
BplI GAGNNNNNCTC 2 cut(s) 5101, 5133
BpmI CTGGAG 6 cut(s) 730, 1317, 4830, 5021, 5103, 5218
Bpu10I CCTNAGC 2 cut(s) 3234, 5118
Bpu1102I GCTNAGC 2 cut(s) 1574, 3642
BpuEI CTTGAG 5 cut(s) 592, 1627, 2783, 3286, 5647
BpuMI CCSGG 2 cut(s) 2533, 4944
Bsa29I ATCGAT 1 cut(s) 3108
BsaBI GATNNNNATC 3 cut(s) 1176, 4356, 5847
BsaI GGTCTC 2 cut(s) 4996, 5160
BsaWI WCCGGW 2 cut(s) 2004, 4070
BsaXI ACNNNNNCTCC 8 cut(s) 772, 802, 1994, 2024, 3946, 3976, 4440, 4470
Bse118I RCCGGY 3 cut(s) 2004, 3866, 4070
Bse3DI GCAATG 4 cut(s) 3618, 3777, 4079, 4245
Bse8I GATNNNNATC 3 cut(s) 1176, 4356, 5847
BseBI CCWGG 4 cut(s) 1009, 4477, 5440, 5889
BseCI ATCGAT 1 cut(s) 3108
BseJI GATNNNNATC 3 cut(s) 1176, 4356, 5847
BseMI GCAATG 4 cut(s) 3618, 3777, 4079, 4245
BseMII CTCAG 9 cut(s) 536, 1256, 1565, 2670, 3656, 3819, 4176, 5132, 5171
BseRI GAGGAG 4 cut(s) 3047, 3437, 4587, 5125
BseSI GKGCMC 1 cut(s) 426
BseYI CCCAGC 3 cut(s) 1932, 2685, 3849
BsgI GTGCAG 4 cut(s) 1752, 1777, 2436, 3582
Bsh1236I CGCG 2 cut(s) 1546, 3625
Bsh1285I CGRYCG 1 cut(s) 1508
BshNI GGYRCC 1 cut(s) 2729
BshTI ACCGGT 2 cut(s) 2004, 4070
BshVI ATCGAT 1 cut(s) 3108
BsiEI CGRYCG 1 cut(s) 1508
BsiHKAI GWGCWC 8 cut(s) 680, 692, 1580, 2183, 3133, 3661, 4717, 5119
BsiHKCI CYCGRG 2 cut(s) 2223, 5745
BsiSI CCGG 6 cut(s) 833, 2005, 2532, 3867, 4071, 4943
BslFI GGGAC 4 cut(s) 1644, 2769, 5809, 5855
BsmAI GTCTC 5 cut(s) 2178, 3262, 4996, 5160, 5555
BsmFI GGGAC 4 cut(s) 1644, 2769, 5809, 5855
BsmI GAATGC 1 cut(s) 2157
Bso31I GGTCTC 2 cut(s) 4996, 5160
BsoBI CYCGRG 2 cut(s) 2223, 5745
Bsp1286I GDGCHC 9 cut(s) 426, 680, 692, 1580, 2183, 3133, 3661, 4717, 5119
Bsp1407I TGTACA 2 cut(s) 1923, 5221
Bsp1720I GCTNAGC 2 cut(s) 1574, 3642
Bsp19I CCATGG 2 cut(s) 2303, 5318
BspCNI CTCAG 9 cut(s) 537, 1257, 1566, 2671, 3655, 3820, 4177, 5131, 5170
BspDI ATCGAT 1 cut(s) 3108
BspFNI CGCG 2 cut(s) 1546, 3625
BspHI TCATGA 4 cut(s) 742, 1530, 2340, 3003
BspMAI CTGCAG 3 cut(s) 1744, 3541, 3556
BspMI ACCTGC 4 cut(s) 629, 1724, 4648, 5263
BspQI GCTCTTC 1 cut(s) 1112
BspT107I GGYRCC 1 cut(s) 2729
BspTI CTTAAG 2 cut(s) 3022, 3301
BspTNI GGTCTC 2 cut(s) 4996, 5160
BsrDI GCAATG 4 cut(s) 3618, 3777, 4079, 4245
BsrFI RCCGGY 3 cut(s) 2004, 3866, 4070
BsrGI TGTACA 2 cut(s) 1923, 5221
BssAI RCCGGY 3 cut(s) 2004, 3866, 4070
BssSI CACGAG 1 cut(s) 5814
Bst2BI CACGAG 1 cut(s) 5814
Bst2UI CCWGG 4 cut(s) 1009, 4477, 5440, 5889
Bst6I CTCTTC 7 cut(s) 1025, 1112, 3112, 3150, 3387, 3414, 5091
BstAFI CTTAAG 2 cut(s) 3022, 3301
BstAPI GCANNNNNTGC 4 cut(s) 1739, 2450, 2624, 3560
BstAUI TGTACA 2 cut(s) 1923, 5221
BstC8I GCNNGC 8 cut(s) 1663, 3523, 3561, 3657, 3722, 4683, 4706, 5595
BstDSI CCRYGG 2 cut(s) 2303, 5318
BstEII GGTNACC 1 cut(s) 1046
BstFNI CGCG 2 cut(s) 1546, 3625
BstHHI GCGC 4 cut(s) 1548, 3625, 5169, 5505
BstMAI GTCTC 5 cut(s) 2178, 3262, 4996, 5160, 5555
BstMCI CGRYCG 1 cut(s) 1508
BstNI CCWGG 4 cut(s) 1009, 4477, 5440, 5889
BstNSI RCATGY 2 cut(s) 1665, 4088
BstPI GGTNACC 1 cut(s) 1046
BstSCI CCNGG 6 cut(s) 1007, 2531, 4475, 4942, 5438, 5887
BstSFI CTRYAG 5 cut(s) 13, 1740, 3537, 3552, 4143
BstSLI GKGCMC 1 cut(s) 426
BstUI CGCG 2 cut(s) 1546, 3625
BstV2I GAAGAC 3 cut(s) 1045, 1780, 2246
BstX2I RGATCY 8 cut(s) 133, 1087, 1126, 1811, 2861, 2947, 5842, 5848
BstYI RGATCY 8 cut(s) 133, 1087, 1126, 1811, 2861, 2947, 5842, 5848
Bsu15I ATCGAT 1 cut(s) 3108
BsuI GTATCC 4 cut(s) 1859, 2770, 3624, 5727
BsuTUI ATCGAT 1 cut(s) 3108
BtgI CCRYGG 2 cut(s) 2303, 5318
BtgZI GCGATG 1 cut(s) 5346
BtrI CACGTC 1 cut(s) 3416
BtsI GCAGTG 5 cut(s) 424, 907, 3561, 3789, 4322
BveI ACCTGC 4 cut(s) 629, 1724, 4648, 5263
Cac8I GCNNGC 8 cut(s) 1663, 3523, 3561, 3657, 3722, 4683, 4706, 5595
CaiI CAGNNNCTG 6 cut(s) 284, 810, 1739, 3726, 3750, 5675
CciI TCATGA 4 cut(s) 742, 1530, 2340, 3003
CfoI GCGC 4 cut(s) 1548, 3625, 5169, 5505
Cfr10I RCCGGY 3 cut(s) 2004, 3866, 4070
ClaI ATCGAT 1 cut(s) 3108
CseI GACGC 2 cut(s) 3483, 3614
CspAI ACCGGT 2 cut(s) 2004, 4070
CspCI CAANNNNNGTGG 4 cut(s) 1203, 1238, 1368, 1403
DraIII CACNNNGTG 1 cut(s) 5651
EaeI YGGCCR 2 cut(s) 3730, 5518
Eam1104I CTCTTC 7 cut(s) 1025, 1112, 3112, 3150, 3387, 3414, 5091
EarI CTCTTC 7 cut(s) 1025, 1112, 3112, 3150, 3387, 3414, 5091
EciI GGCGGA 1 cut(s) 3074
Eco147I AGGCCT 2 cut(s) 784, 4681
Eco31I GGTCTC 2 cut(s) 4996, 5160
Eco32I GATATC 1 cut(s) 1198
Eco47I GGWCC 3 cut(s) 104, 2403, 3337
Eco57I CTGAAG 4 cut(s) 615, 3647, 4323, 5417
Eco88I CYCGRG 2 cut(s) 2223, 5745
Eco91I GGTNACC 1 cut(s) 1046
EcoO109I RGGNCCY 2 cut(s) 104, 2403
EcoO65I GGTNACC 1 cut(s) 1046
EcoRII CCWGG 4 cut(s) 1007, 4475, 5438, 5887
EcoRV GATATC 1 cut(s) 1198
EcoT22I ATGCAT 3 cut(s) 1915, 4159, 4200
FaqI GGGAC 4 cut(s) 1644, 2769, 5809, 5855
FauI CCCGC 2 cut(s) 5083, 5586
FauNDI CATATG 1 cut(s) 5011
FbaI TGATCA 4 cut(s) 3912, 4357, 4591, 4612
FblI GTMKAC 2 cut(s) 4768, 5073
GlaI GCGC 4 cut(s) 1547, 3624, 5168, 5504
GsaI CCCAGC 3 cut(s) 1936, 2689, 3853
GsuI CTGGAG 6 cut(s) 730, 1317, 4830, 5021, 5103, 5218
HapII CCGG 6 cut(s) 833, 2005, 2532, 3867, 4071, 4943
HgaI GACGC 2 cut(s) 3483, 3614
HhaI GCGC 4 cut(s) 1548, 3625, 5169, 5505
Hin6I GCGC 4 cut(s) 1546, 3623, 5167, 5503
HinP1I GCGC 4 cut(s) 1546, 3623, 5167, 5503
HincII GTYRAC 1 cut(s) 4769
HindII GTYRAC 1 cut(s) 4769
HindIII AAGCTT 4 cut(s) 1894, 2216, 5037, 5297
HpaII CCGG 6 cut(s) 833, 2005, 2532, 3867, 4071, 4943
Hpy99I CGWCG 1 cut(s) 3630
HpyCH4IV ACGT 6 cut(s) 256, 619, 845, 3415, 4441, 4525
HpySE526I ACGT 6 cut(s) 256, 619, 845, 3415, 4441, 4525
HspAI GCGC 4 cut(s) 1546, 3623, 5167, 5503
Ksp22I TGATCA 4 cut(s) 3912, 4357, 4591, 4612
LguI GCTCTTC 1 cut(s) 1112
MaeII ACGT 6 cut(s) 256, 619, 845, 3415, 4441, 4525
MfeI CAATTG 3 cut(s) 1380, 1392, 4008
MflI RGATCY 8 cut(s) 133, 1087, 1126, 1811, 2861, 2947, 5842, 5848
MhlI GDGCHC 9 cut(s) 426, 680, 692, 1580, 2183, 3133, 3661, 4717, 5119
MlsI TGGCCA 1 cut(s) 3732
MluNI TGGCCA 1 cut(s) 3732
MlyI GAGTC 5 cut(s) 245, 1205, 3264, 4196, 5351
MmeI TCCRAC 6 cut(s) 1154, 1567, 1706, 2443, 2923, 3608
Mox20I TGGCCA 1 cut(s) 3732
Mph1103I ATGCAT 3 cut(s) 1915, 4159, 4200
MroXI GAANNNNTTC 1 cut(s) 2899
MscI TGGCCA 1 cut(s) 3732
MseI TTAA 9 cut(s) 339, 753, 1062, 2150, 3023, 3302, 3500, 4232, 5808
MslI CAYNNNNRTG 5 cut(s) 884, 1835, 2957, 3782, 4286
Msp20I TGGCCA 1 cut(s) 3732
MspA1I CMGCKG 8 cut(s) 281, 2282, 2735, 2930, 3542, 3551, 4265, 4277
MspCI CTTAAG 2 cut(s) 3022, 3301
MspI CCGG 6 cut(s) 833, 2005, 2532, 3867, 4071, 4943
MspR9I CCNGG 6 cut(s) 1009, 2533, 4477, 4944, 5440, 5889
MunI CAATTG 3 cut(s) 1380, 1392, 4008
Mva1269I GAATGC 1 cut(s) 2157
MvaI CCWGG 4 cut(s) 1009, 4477, 5440, 5889
MvnI CGCG 2 cut(s) 1546, 3625
NciI CCSGG 2 cut(s) 2533, 4944
NcoI CCATGG 2 cut(s) 2303, 5318
NdeI CATATG 1 cut(s) 5011
NmeAIII GCCGAG 3 cut(s) 3631, 4696, 5780
NmuCI GTSAC 4 cut(s) 1046, 1184, 2123, 5291
NsiI ATGCAT 3 cut(s) 1915, 4159, 4200
NspI RCATGY 2 cut(s) 1665, 4088
OliI CACNNNNGTG 2 cut(s) 884, 1835
PaeI GCATGC 1 cut(s) 1665
PagI TCATGA 4 cut(s) 742, 1530, 2340, 3003
PaqCI CACCTGC 1 cut(s) 5263
PceI AGGCCT 2 cut(s) 784, 4681
PciSI GCTCTTC 1 cut(s) 1112
PctI GAATGC 1 cut(s) 2157
PdmI GAANNNNTTC 1 cut(s) 2899
PflMI CCANNNNNTGG 1 cut(s) 2726
PinAI ACCGGT 2 cut(s) 2004, 4070
Ple19I CGATCG 1 cut(s) 1508
PleI GAGTC 5 cut(s) 245, 1205, 3264, 4195, 5351
PpsI GAGTC 5 cut(s) 245, 1205, 3264, 4195, 5351
PpuMI RGGWCCY 2 cut(s) 104, 2403
PshBI ATTAAT 1 cut(s) 753
Psp5II RGGWCCY 2 cut(s) 104, 2403
Psp6I CCWGG 4 cut(s) 1007, 4475, 5438, 5887
PspEI GGTNACC 1 cut(s) 1046
PspFI CCCAGC 3 cut(s) 1932, 2685, 3849
PspGI CCWGG 4 cut(s) 1007, 4475, 5438, 5887
PspPPI RGGWCCY 2 cut(s) 104, 2403
PsrI GAACNNNNNNTAC 2 cut(s) 835, 867
PstI CTGCAG 3 cut(s) 1744, 3541, 3556
PstNI CAGNNNCTG 6 cut(s) 284, 810, 1739, 3726, 3750, 5675
PsuI RGATCY 8 cut(s) 133, 1087, 1126, 1811, 2861, 2947, 5842, 5848
PvuI CGATCG 1 cut(s) 1508
PvuII CAGCTG 7 cut(s) 281, 2282, 2930, 3542, 3551, 4265, 4277
RseI CAYNNNNRTG 5 cut(s) 884, 1835, 2957, 3782, 4286
SalI GTCGAC 1 cut(s) 4767
SapI GCTCTTC 1 cut(s) 1112
SaqAI TTAA 9 cut(s) 339, 753, 1062, 2150, 3023, 3302, 3500, 4232, 5808
ScaI AGTACT 1 cut(s) 532
SchI GAGTC 5 cut(s) 245, 1205, 3264, 4196, 5351
ScrFI CCNGG 6 cut(s) 1009, 2533, 4477, 4944, 5440, 5889
SduI GDGCHC 9 cut(s) 426, 680, 692, 1580, 2183, 3133, 3661, 4717, 5119
SfcI CTRYAG 5 cut(s) 13, 1740, 3537, 3552, 4143
SinI GGWCC 3 cut(s) 104, 2403, 3337
SmiMI CAYNNNNRTG 5 cut(s) 884, 1835, 2957, 3782, 4286
SmlI CTYRAG 7 cut(s) 607, 1606, 2798, 3022, 3265, 3301, 5662
SmoI CTYRAG 7 cut(s) 607, 1606, 2798, 3022, 3265, 3301, 5662
SpeI ACTAGT 3 cut(s) 1057, 3968, 4283
SphI GCATGC 1 cut(s) 1665
SseBI AGGCCT 2 cut(s) 784, 4681
SspI AATATT 4 cut(s) 384, 3144, 3858, 4412
StuI AGGCCT 2 cut(s) 784, 4681
StyD4I CCNGG 6 cut(s) 1007, 2531, 4475, 4942, 5438, 5887
TaiI ACGT 6 cut(s) 259, 622, 848, 3418, 4444, 4528
TatI WGTACW 8 cut(s) 219, 530, 1816, 1923, 1972, 2347, 3247, 5221
TauI GCSGC 3 cut(s) 1546, 2735, 5754
Tru1I TTAA 9 cut(s) 339, 753, 1062, 2150, 3023, 3302, 3500, 4232, 5808
Tru9I TTAA 9 cut(s) 339, 753, 1062, 2150, 3023, 3302, 3500, 4232, 5808
TseFI GTSAC 4 cut(s) 1046, 1184, 2123, 5291
Tsp45I GTSAC 4 cut(s) 1046, 1184, 2123, 5291
TspGWI ACGGA 3 cut(s) 1388, 4250, 4826
Van91I CCANNNNNTGG 1 cut(s) 2726
Vha464I CTTAAG 2 cut(s) 3022, 3301
VpaK11BI GGWCC 3 cut(s) 104, 2403, 3337
VspI ATTAAT 1 cut(s) 753
XbaI TCTAGA 3 cut(s) 2096, 3694, 4334
XceI RCATGY 2 cut(s) 1665, 4088
XcmI CCANNNNNNNNNTGG 2 cut(s) 1341, 4265
XmaJI CCTAGG 1 cut(s) 4493
XmiI GTMKAC 2 cut(s) 4768, 5073
XmnI GAANNNNTTC 1 cut(s) 2899
ZrmI AGTACT 1 cut(s) 532
Zsp2I ATGCAT 3 cut(s) 1915, 4159, 4200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.