Rmu_sc0001287.1_g000035

Belongs to the peptidase C2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001287.1
OG0007783
Physical Location & Seq
Reverse (-)
167271 .. 182159
14889 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001287.1_g000035.1.cds

Sequence Viewer

Length: 5691 bp
atggaaggagatgagcgtcacgtgctgctcgcatgcgtcatttcaggaacccttttctccgttctgggttctgcttcattttctatactctggcttgtgaattggaggccgtggaggatctacagttggatattcgccaggaaatggccagatatcttacacggaccccagctggatatagtatgtggttttctgtcactctctgcatggattcttgtcatttctccagttcttgtactaatcatatggggaagctggttggttgtaatattggatcgacatataattggtctggctgttattatggctggtactgctcttctactggctttctattcaatcatgctttggtggcgaacgcaatggcagagctcaagggctgttgctattcttcttcttcttgctgttgcccttctctgtgcctatgaactctgtgctgtatatgttacagctggttcaaaagcatctcagcggtattcgccttctggtttcttttttggtgtatcagcaattgccttggcaatcaacatgctctttatctgtagaatggtgtttaatggaaatggcttagatgtcgatgagtatgtgaggaaggcatacaaatttgcttattctgattgtatagaagtaggtcctgtggcctgtttaccagaaccaccagatccgaatgagttatatcctcgacaatctagcagggcttcacatcttgggcttctttaccttggctcacttgtagttcttctcgtgtactcaatcctatatggacttactgcaaaggacgcacgttggcttggagccattacatcagctgctgtgataattcttgattggaacatgggagcatgtttatatggattcgaactccttaatagtcgtgtagcagcattatttgttgccggcacatctcgaatttttctcatttgcttcggcgttcactactggtatttggggcactgcattagttatgcagttgtagcatctgttttattaggtgcttctgtttctcgtcatttatctgtaacaaacccattggctgcaaggagagatgccttacagagtactgttattcgcctcagagagggtttccgcaaaaaagaacagaacagttcatcaagctcatctgaaggttgtggctcaagcatgaaacgcagtagtagtgttgaagcaggttgccttggtaatgttgttgaagcaagtcacaggagcgcaacacacagcacggttgatgctaataactggagtaatgttctatttcgaactgcgagttctcacgaggggattaatagtgataaaagcatagatagtgggaggccaagtatagcattgtgtagcagttcctgtcgttcagtaatccaagagcctgaagtgggaacttcctttactgacaaaaattgcgatcaaaatagcgcattggtggtttgttctagcagtggtcttgaaagccaaggctgtgaatccagtgcatcaaattctgcaagtcaacaaactttagatttgaatttagcttttgcattgcaagaaaggttgaatgaccccaggataacatctatgctaaagaagaggggaagacaaagtgaccttgaactagttaatcttttgcaggataaaggactggatcctaactttgttatgatgttgaaggagaagagcttggatccaaccattctggcattacttcaaagaagtagtttggatgctgatagagatcatcgtgacaatacagatatcaccatagctgactcaaacagtgtggataatggtttacccaatcaaatctctttatcagaagaactgaggcttcatgggcttgaaaaatggctccaattatctaggcttgttttgcaccatgtagtaggtaccccagagagagcatgggttctctttagttttgttttcatcctcgaaacaattgctgtggcaattgtccgtccaaagataatcaaaataataaatgcgacacatcaacagtttgaatttgggtttgcggtgctgctgttatctcctgtggtctgttcaatcatggcttttcttcgatctcttcaagcagaagaaatggccatgacctctaagccacgcaagtatggttttgttgcttggctgctgagcacctgcgttggattattgctttccttcttgagcaaatcatcagttcttctgggattgtctttgactgttcccctcatggtggcatgcctttctgttgccattcctacatggatccgtaatggctaccaattttgggttcctcaattacattgtgcaggtcccgcagggaaccatcaaattcggggaacaaaagagggtgttattcttgtcttctgcacaacattatttactggatctgtattagctctcggtgtaatagtgtctgcaaagcccttggatgacttaaattacaaaggatggacgggtgaacagaaaagctttacatctccttatgcatcatctgtgtacattggctgggctatggcttctgccattgctttagtggttactggtgtactgccaattgtttcatggtttgcatcttaccggttctccatgttttctgctgtgtgtgtcgggatatttacagctgtcctcgtgtcattttgtggtgcatcctatattgaggttgtgaaatctagagatgaccaagttccaacaaagggtgatttccttgctgctttacttcctttaatttgcattccagcatttctgtctctgtgctcggggttgtataaatggaaggatgataattggaagctttctcggggtgtttatatctttgtaaccatcggtcttctacttctgctcggagcaatatcagctgttatagtcgtagttacaccatggacggttggggtatcatttcttttagttcttctcatgattgtactggctattggtgctatacatcattgggcctcaaacaacttttatttgacaaggacccagacattctttgtctgtttcctcgcttttcttttggcactggctgcacttcttgttggatggtttgaagaaaaaccatttgtcggtgcttctgtcggctactttttgtttttgtttcttcttgccgggagggcattgactgttcttctttcacctcctatagttgtttattctccaagagttctccctgtttatgtttatgatgctcatgcagactgtgcaaagaatgtcagtgctgcatttcttgtgctttatgggattgcattggcaactgagggctggggtgttgttgcgagtttgaaaatttatccaccatttgctggtgccgctgtatcagcaattactcttgttgctgcctttgggtttgctttctctcgtccctgcttgactctcaagatgatggaagatgcggtacactttcttagtaaagaaaccgttgttcaagctattgctagatctgccaccaagacaggaatttccggagctgtatgcgttctagatgatgaaccgacaacaagtggcaggcactgtggccagattgattctagcatctgtcaaagtcagaagatcagcttttccattgcagtgatgatccaacctgtgtctgggcctgtttgcctgttaggcactgagtttcaaaagaaagtttgttgggaaatattggtcgcaggttctgagcaaggaattgaagctggacaagttgggcttagattgatcactaaaggcgacagacaaactactgttgcaaaggagtggagtattagtgcaactagtattgcagatggaaggtggcatttggtaacaatgacaattgatgctgatttaggtgaggcaacttgctatctcgatggtggttttgatggctaccagactggtttaccattgcatgtgggtaatactatttgggagcaaggaacagaagtttgggtaggtgttagaccgcctacagatatggatgcatttgggaggtcagacagtgaaggggctgagtccaagatgcatattatggatgtgtttctatggggaaggtgcttaactgaggatgatattgctgctctccatacagctgttgggtcagctgatactagtatgatagattttcctgaagatgcttggcagtgggcagattcaccttctagacttgatgaatgggatagtgatcctgccgatgtagatctatatgatagggatgaagttgattgggatgggcaatattctagtggaaggaaaagaagatcagagcgtgatggagttctggttgatttggattcctttgccaggaggtttaggaaacctaggatggaaacgcaagaagaaattaatcaacgaatgctttctgttgaattggccgtcaaagaagctctctgtgcaagaggagaaacaaattttactgatcaggagtttcctccaaatgatcaatcactatttgtagattcggaaaatcccccttcaaagttgcaggtcgtttcagaatggatgaggcctgctgatattgtaaaagagagcaggcttgctactcggccgtgcttattttctggcactgtgaatccttctgatgtttgtcagggtcggctgggtgattgttggtttttgagtgctgttgctgttctgacggaagtttctcgaatatcagaagttattattactccagagtataatgaggaaggaatctacactgtccgcttctgtattcagggtgagtgggttcctgttgttgttgatgattggattccgtgtgaatcaccgggtaaaccagcatttgctactagtagaaaaggtaacgagttgtgggtctctttactggagaaggcatatgccaaattgcatggctcctatgaagcattagaaggtggacttgtccaagatgctcttgtagacctcactggaggtgcaggtgaagaaattgatatgaggagtgctcaggcccaaattgatcttgcgagtggtcgattatggtctcaattgttgcgcttcaaacaagagggatttcttctgggtgctggaagtccatctggttcagatgtacatatttcctccagtggcattgtgcaaggtcatgcttactcgttattgcaggtgagagaggtggatggtcacaagcttattcagattcgcaatccatgggccaatgaagttgagtggaatggcccctggtctgactcatcgcctgaatggactgataggatgaagcataagctgaagcatattccacagtcaaaagatggcatattctggatgtcctggcaagacttccaaattcattttcgatcaatatatgtttgccgtatctatccccctgagatgcgctactctgttcatggccaatggcgaagttatagtgctggtgggtgccaagactatgagacatggcatcaaaatccacaattccgattgagggccacagggcgagatgcctcatttccaattcatgtattcattacgttgacacagggtgtgagcttctcaagaacagttgctggtttcagaaactatcaatccagtcatgattcaatgatgttctatattgggatgaggatactcaaaactcgaggtcgccgagctgcttacaatatatacctacatgaatcagtcggcgggacagattatgttaattcacgagaaatatcttgtgaaatggttttggatccagatccaaaaggttacacaatagtgccgacaactatacaccctggcgaagaagctccatttgttctttctgttttcacaaaagcatcaataaccttggaagccttgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001558 GO:0001708 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0004198 GO:0005198 GO:0005488 GO:0005509 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0005829 GO:0005886 GO:0005887 GO:0006275 GO:0006355 GO:0006508 GO:0006807 GO:0006873 GO:0006874 GO:0006875 GO:0006950 GO:0006970 GO:0006996 GO:0007010 GO:0007204 GO:0007275 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0008307 GO:0009628 GO:0009651 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009934 GO:0009966 GO:0009987 GO:0010008 GO:0010035 GO:0010038 GO:0010154 GO:0010468 GO:0010522 GO:0010524 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010646 GO:0010927 GO:0010941 GO:0010959 GO:0012505 GO:0014717 GO:0014718 GO:0014823 GO:0014850 GO:0016020 GO:0016021 GO:0016043 GO:0016787 GO:0019219 GO:0019222 GO:0019538 GO:0019725 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0030003 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030036 GO:0030154 GO:0030162 GO:0030234 GO:0030239 GO:0030315 GO:0031032 GO:0031090 GO:0031224 GO:0031226 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031402 GO:0031410 GO:0031420 GO:0031432 GO:0031674 GO:0031982 GO:0031984 GO:0032101 GO:0032268 GO:0032269 GO:0032270 GO:0032501 GO:0032502 GO:0032875 GO:0032877 GO:0032879 GO:0032947 GO:0032989 GO:0032991 GO:0033036 GO:0033233 GO:0033234 GO:0033554 GO:0034613 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0040008 GO:0042127 GO:0042175 GO:0042221 GO:0042383 GO:0042592 GO:0042692 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043269 GO:0043270 GO:0043292 GO:0043415 GO:0043416 GO:0043933 GO:0044085 GO:0044093 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044449 GO:0044459 GO:0044464 GO:0045165 GO:0045214 GO:0045740 GO:0045787 GO:0045862 GO:0045892 GO:0045893 GO:0045927 GO:0045934 GO:0045935 GO:0046872 GO:0048316 GO:0048468 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048583 GO:0048584 GO:0048608 GO:0048638 GO:0048639 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050801 GO:0050865 GO:0050867 GO:0050896 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051146 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051239 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051279 GO:0051281 GO:0051282 GO:0051480 GO:0051592 GO:0051641 GO:0051716 GO:0051726 GO:0051924 GO:0051928 GO:0055001 GO:0055002 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055103 GO:0060255 GO:0060341 GO:0060548 GO:0061041 GO:0061061 GO:0061458 GO:0065003 GO:0065007 GO:0065008 GO:0065009 GO:0070011 GO:0070727 GO:0070887 GO:0070925 GO:0071214 GO:0071241 GO:0071248 GO:0071277 GO:0071470 GO:0071472 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072657 GO:0080050 GO:0080090 GO:0080134 GO:0090068 GO:0090303 GO:0090329 GO:0090392 GO:0090627 GO:0090628 GO:0097264 GO:0097435 GO:0097708 GO:0098588 GO:0098771 GO:0098772 GO:0098805 GO:0098827 GO:0099080 GO:0099081 GO:0099512 GO:0104004 GO:0140096 GO:1901564 GO:1902531 GO:1902679 GO:1902680 GO:1903034 GO:1903036 GO:1903169 GO:1903320 GO:1903321 GO:1903506 GO:1903507 GO:1903508 GO:1904062 GO:1904064 GO:1904427 GO:2000011 GO:2000014 GO:2000024 GO:2000026 GO:2000105 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calpain_III Peptidase_C2

Protein Analysis

1896

Amino Acids

208.93

Weight (kDa)

5.83

Isoelectric Point (pI)

38.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 2117, 4817, 4999
Acc36I ACCTGC 7 cut(s) 1157, 2117, 2252, 3593, 4384, 4817, 4999
Acc65I GGTACC 1 cut(s) 1856
AccB1I GGYRCC 3 cut(s) 1856, 3257, 5283
AccB7I CCANNNNNTGG 2 cut(s) 144, 3254
AccI GTMKAC 1 cut(s) 4809
AccIII TCCGGA 1 cut(s) 3413
AciI CCGC 9 cut(s) 472, 1087, 1985, 2268, 3261, 3344, 3875, 4615, 5529
AcoI YGGCCR 6 cut(s) 146, 2055, 3466, 4281, 4454, 5254
AcuI CTGAAG 4 cut(s) 1143, 1383, 4059, 5153
AcvI CACGTG 1 cut(s) 22
AdeI CACNNNGTG 1 cut(s) 5387
AgeI ACCGGT 1 cut(s) 2532
AhlI ACTAGT 4 cut(s) 1585, 3704, 4019, 4700
AjnI CCWGG 6 cut(s) 137, 1535, 4211, 5084, 5174, 5623
AjuI GAANNNNNNNTTGG 6 cut(s) 2226, 2258, 3841, 3873, 5181, 5213
AleI CACNNNNGTG 1 cut(s) 5603
Alw21I GWGCWC 4 cut(s) 374, 2108, 2711, 4855
Alw26I GTCTC 4 cut(s) 2706, 4732, 4896, 5291
AlwNI CAGNNNCTG 5 cut(s) 812, 1338, 3462, 3608, 5411
Ama87I CYCGRG 3 cut(s) 2710, 2751, 5481
Aor13HI TCCGGA 1 cut(s) 3413
AseI ATTAAT 2 cut(s) 1281, 4254
AsiGI ACCGGT 1 cut(s) 2532
Asp700I GAANNNNTTC 1 cut(s) 4267
Asp718I GGTACC 1 cut(s) 1856
AspA2I CCTAGG 1 cut(s) 4229
AspLEI GCGC 4 cut(s) 1208, 1409, 4905, 5241
AsuC2I CCSGG 2 cut(s) 3061, 4680
AsuII TTCGAA 2 cut(s) 858, 1255
AvaI CYCGRG 3 cut(s) 2710, 2751, 5481
AvaII GGWCC 4 cut(s) 164, 632, 2264, 2931
AvrII CCTAGG 1 cut(s) 4229
BaeGI GKGCMC 1 cut(s) 954
BalI TGGCCA 4 cut(s) 148, 2057, 3468, 5256
BamHI GGATCC 4 cut(s) 1615, 1654, 2217, 5578
BanI GGYRCC 3 cut(s) 1856, 3257, 5283
BanII GRGCYC 1 cut(s) 374
BarI GAAGNNNNNNTAC 2 cut(s) 3330, 3362
BauI CACGAG 4 cut(s) 743, 1271, 2582, 5550
BbrPI CACGTG 1 cut(s) 22
BbsI GAAGAC 3 cut(s) 1573, 2308, 2774
Bbv12I GWGCWC 4 cut(s) 374, 2108, 2711, 4855
BceAI ACGGC 4 cut(s) 94, 4268, 4441, 5202
BcgI CGANNNNNNTGC 2 cut(s) 1892, 1926
BciT130I CCWGG 6 cut(s) 139, 1537, 4213, 5086, 5176, 5625
BciVI GTATCC 1 cut(s) 5463
BclI TGATCA 3 cut(s) 3648, 4327, 4348
BcnI CCSGG 2 cut(s) 3061, 4680
BcoDI GTCTC 4 cut(s) 2706, 4732, 4896, 5291
BcuI ACTAGT 4 cut(s) 1585, 3704, 4019, 4700
BfmI CTRYAG 4 cut(s) 121, 541, 3093, 3879
BfuAI ACCTGC 7 cut(s) 1157, 2117, 2252, 3593, 4384, 4817, 4999
BfuI GTATCC 1 cut(s) 5463
BglI GCCNNNNNGGC 2 cut(s) 3065, 3558
BglII AGATCT 2 cut(s) 3389, 4108
BlnI CCTAGG 1 cut(s) 4229
BlpI GCTNAGC 1 cut(s) 2102
BmcAI AGTACT 1 cut(s) 1060
Bme1390I CCNGG 8 cut(s) 139, 1537, 3061, 4213, 4680, 5086, 5176, 5625
Bme18I GGWCC 4 cut(s) 164, 632, 2264, 2931
BmeT110I CYCGRG 3 cut(s) 2710, 2751, 5481
BmrFI CCNGG 8 cut(s) 139, 1537, 3061, 4213, 4680, 5086, 5176, 5625
BpiI GAAGAC 3 cut(s) 1573, 2308, 2774
BplI GAGNNNNNCTC 2 cut(s) 4837, 4869
BpmI CTGGAG 6 cut(s) 210, 1258, 4566, 4757, 4839, 4954
Bpu10I CCTNAGC 1 cut(s) 4854
Bpu1102I GCTNAGC 1 cut(s) 2102
Bpu14I TTCGAA 2 cut(s) 858, 1255
BpuEI CTTGAG 5 cut(s) 358, 1120, 2155, 3311, 5383
BpuMI CCSGG 2 cut(s) 3061, 4680
BsaAI YACGTR 1 cut(s) 22
BsaBI GATNNNNATC 4 cut(s) 1704, 4092, 4107, 5583
BsaI GGTCTC 2 cut(s) 4732, 4896
BsaWI WCCGGW 2 cut(s) 2532, 3413
BsaXI ACNNNNNCTCC 8 cut(s) 1300, 1330, 2522, 2552, 3682, 3712, 4176, 4206
Bse118I RCCGGY 2 cut(s) 896, 2532
Bse3DI GCAATG 5 cut(s) 368, 1511, 2478, 3513, 3815
Bse8I GATNNNNATC 4 cut(s) 1704, 4092, 4107, 5583
BseAI TCCGGA 1 cut(s) 3413
BseBI CCWGG 6 cut(s) 139, 1537, 4213, 5086, 5176, 5625
BseJI GATNNNNATC 4 cut(s) 1704, 4092, 4107, 5583
BseMI GCAATG 5 cut(s) 368, 1511, 2478, 3513, 3815
BseRI GAGGAG 2 cut(s) 4323, 4861
BseSI GKGCMC 1 cut(s) 954
BseX3I CGGCCG 1 cut(s) 4454
BseYI CCCAGC 4 cut(s) 168, 2460, 3213, 4507
BsgI GTGCAG 4 cut(s) 2280, 2305, 2964, 4845
Bsh1285I CGRYCG 1 cut(s) 4457
BshNI GGYRCC 3 cut(s) 1856, 3257, 5283
BshTI ACCGGT 1 cut(s) 2532
BsiEI CGRYCG 1 cut(s) 4457
BsiHKAI GWGCWC 4 cut(s) 374, 2108, 2711, 4855
BsiHKCI CYCGRG 3 cut(s) 2710, 2751, 5481
BsiSI CCGG 5 cut(s) 897, 2533, 3060, 3414, 4679
BslFI GGGAC 3 cut(s) 2250, 3297, 5545
BsmAI GTCTC 4 cut(s) 2706, 4732, 4896, 5291
BsmFI GGGAC 3 cut(s) 2250, 3297, 5545
BsmI GAATGC 2 cut(s) 2685, 4269
Bso31I GGTCTC 2 cut(s) 4732, 4896
BsoBI CYCGRG 3 cut(s) 2710, 2751, 5481
Bsp119I TTCGAA 2 cut(s) 858, 1255
Bsp1286I GDGCHC 5 cut(s) 374, 954, 2108, 2711, 4855
Bsp13I TCCGGA 1 cut(s) 3413
Bsp1407I TGTACA 2 cut(s) 2451, 4957
Bsp1720I GCTNAGC 1 cut(s) 2102
Bsp19I CCATGG 2 cut(s) 2831, 5054
BspACI CCGC 9 cut(s) 472, 1087, 1985, 2268, 3261, 3344, 3875, 4615, 5529
BspEI TCCGGA 1 cut(s) 3413
BspHI TCATGA 2 cut(s) 2868, 5437
BspMI ACCTGC 7 cut(s) 1157, 2117, 2252, 3593, 4384, 4817, 4999
BspQI GCTCTTC 2 cut(s) 324, 1640
BspT104I TTCGAA 2 cut(s) 858, 1255
BspT107I GGYRCC 3 cut(s) 1856, 3257, 5283
BspTNI GGTCTC 2 cut(s) 4732, 4896
BsrDI GCAATG 5 cut(s) 368, 1511, 2478, 3513, 3815
BsrFI RCCGGY 2 cut(s) 896, 2532
BsrGI TGTACA 2 cut(s) 2451, 4957
BssAI RCCGGY 2 cut(s) 896, 2532
BssSI CACGAG 4 cut(s) 743, 1271, 2582, 5550
BssT1I CCWWGG 9 cut(s) 516, 721, 1174, 1444, 2379, 2831, 4229, 5054, 5676
Bst2BI CACGAG 4 cut(s) 743, 1271, 2582, 5550
Bst2UI CCWGG 6 cut(s) 139, 1537, 4213, 5086, 5176, 5625
Bst6I CTCTTC 4 cut(s) 324, 1553, 1640, 2043
BstAPI GCANNNNNTGC 2 cut(s) 2978, 3152
BstAUI TGTACA 2 cut(s) 2451, 4957
BstBAI YACGTR 1 cut(s) 22
BstBI TTCGAA 2 cut(s) 858, 1255
BstC8I GCNNGC 8 cut(s) 30, 34, 898, 2191, 3458, 4419, 4442, 4446
BstDSI CCRYGG 3 cut(s) 110, 2831, 5054
BstENI CCTNNNNNAGG 2 cut(s) 1076, 4210
BstHHI GCGC 4 cut(s) 1208, 1409, 4905, 5241
BstMAI GTCTC 4 cut(s) 2706, 4732, 4896, 5291
BstMCI CGRYCG 1 cut(s) 4457
BstNI CCWGG 6 cut(s) 139, 1537, 4213, 5086, 5176, 5625
BstNSI RCATGY 5 cut(s) 36, 532, 846, 2193, 3824
BstSCI CCNGG 8 cut(s) 137, 1535, 3059, 4211, 4678, 5084, 5174, 5623
BstSFI CTRYAG 4 cut(s) 121, 541, 3093, 3879
BstSLI GKGCMC 1 cut(s) 954
BstV2I GAAGAC 3 cut(s) 1573, 2308, 2774
BstZI CGGCCG 1 cut(s) 4454
BsuI GTATCC 1 cut(s) 5463
BtgI CCRYGG 3 cut(s) 110, 2831, 5054
BtgZI GCGATG 1 cut(s) 5082
BtsI GCAGTG 4 cut(s) 952, 1435, 3525, 4058
BveI ACCTGC 7 cut(s) 1157, 2117, 2252, 3593, 4384, 4817, 4999
Cac8I GCNNGC 8 cut(s) 30, 34, 898, 2191, 3458, 4419, 4442, 4446
CaiI CAGNNNCTG 5 cut(s) 812, 1338, 3462, 3608, 5411
CciI TCATGA 2 cut(s) 2868, 5437
CfoI GCGC 4 cut(s) 1208, 1409, 4905, 5241
Cfr10I RCCGGY 2 cut(s) 896, 2532
CseI GACGC 3 cut(s) 5, 25, 788
CspAI ACCGGT 1 cut(s) 2532
CspCI CAANNNNNGTGG 4 cut(s) 1731, 1766, 1896, 1931
DraIII CACNNNGTG 1 cut(s) 5387
EaeI YGGCCR 6 cut(s) 146, 2055, 3466, 4281, 4454, 5254
EagI CGGCCG 1 cut(s) 4454
Eam1104I CTCTTC 4 cut(s) 324, 1553, 1640, 2043
EarI CTCTTC 4 cut(s) 324, 1553, 1640, 2043
Ecl136II GAGCTC 1 cut(s) 372
EclXI CGGCCG 1 cut(s) 4454
Eco130I CCWWGG 9 cut(s) 516, 721, 1174, 1444, 2379, 2831, 4229, 5054, 5676
Eco147I AGGCCT 1 cut(s) 4417
Eco24I GRGCYC 1 cut(s) 374
Eco31I GGTCTC 2 cut(s) 4732, 4896
Eco32I GATATC 2 cut(s) 154, 1726
Eco47I GGWCC 4 cut(s) 164, 632, 2264, 2931
Eco52I CGGCCG 1 cut(s) 4454
Eco53kI GAGCTC 1 cut(s) 372
Eco57I CTGAAG 4 cut(s) 1143, 1383, 4059, 5153
Eco72I CACGTG 1 cut(s) 22
Eco88I CYCGRG 3 cut(s) 2710, 2751, 5481
EcoICRI GAGCTC 1 cut(s) 372
EcoNI CCTNNNNNAGG 2 cut(s) 1076, 4210
EcoO109I RGGNCCY 3 cut(s) 632, 2264, 2931
EcoRII CCWGG 6 cut(s) 137, 1535, 4211, 5084, 5174, 5623
EcoRV GATATC 2 cut(s) 154, 1726
EcoT14I CCWWGG 9 cut(s) 516, 721, 1174, 1444, 2379, 2831, 4229, 5054, 5676
EcoT22I ATGCAT 3 cut(s) 2443, 3895, 3936
EcoT38I GRGCYC 1 cut(s) 374
ErhI CCWWGG 9 cut(s) 516, 721, 1174, 1444, 2379, 2831, 4229, 5054, 5676
FalI AAGNNNNNCTT 8 cut(s) 3491, 3523, 3624, 3656, 4773, 4805, 4788, 4820
FaqI GGGAC 3 cut(s) 2250, 3297, 5545
FauI CCCGC 2 cut(s) 2275, 5522
FauNDI CATATG 2 cut(s) 245, 4747
FbaI TGATCA 3 cut(s) 3648, 4327, 4348
FblI GTMKAC 1 cut(s) 4809
FriOI GRGCYC 1 cut(s) 374
GlaI GCGC 4 cut(s) 1207, 1408, 4904, 5240
GsaI CCCAGC 4 cut(s) 172, 2464, 3217, 4511
GsuI CTGGAG 6 cut(s) 210, 1258, 4566, 4757, 4839, 4954
HapII CCGG 5 cut(s) 897, 2533, 3060, 3414, 4679
HgaI GACGC 3 cut(s) 5, 25, 788
HhaI GCGC 4 cut(s) 1208, 1409, 4905, 5241
Hin6I GCGC 4 cut(s) 1206, 1407, 4903, 5239
HinP1I GCGC 4 cut(s) 1206, 1407, 4903, 5239
HincII GTYRAC 2 cut(s) 1481, 5379
HindII GTYRAC 2 cut(s) 1481, 5379
HindIII AAGCTT 3 cut(s) 2422, 2744, 5033
HpaII CCGG 5 cut(s) 897, 2533, 3060, 3414, 4679
HpyCH4IV ACGT 3 cut(s) 21, 784, 5375
HpySE526I ACGT 3 cut(s) 21, 784, 5375
HspAI GCGC 4 cut(s) 1206, 1407, 4903, 5239
Kpn2I TCCGGA 1 cut(s) 3413
KpnI GGTACC 1 cut(s) 1860
KroI GCCGGC 1 cut(s) 896
KroNI GCCGGC 1 cut(s) 898
Ksp22I TGATCA 3 cut(s) 3648, 4327, 4348
LguI GCTCTTC 2 cut(s) 324, 1640
LmnI GCTCC 9 cut(s) 794, 839, 1203, 1824, 2798, 3416, 3841, 4769, 5641
MaeII ACGT 3 cut(s) 21, 784, 5375
MfeI CAATTG 6 cut(s) 510, 1908, 1920, 2508, 3744, 4895
MhlI GDGCHC 5 cut(s) 374, 954, 2108, 2711, 4855
MlsI TGGCCA 4 cut(s) 148, 2057, 3468, 5256
MluNI TGGCCA 4 cut(s) 148, 2057, 3468, 5256
MlyI GAGTC 4 cut(s) 1733, 3316, 3932, 5087
MmeI TCCRAC 6 cut(s) 107, 1682, 2095, 2666, 2971, 3553
Mox20I TGGCCA 4 cut(s) 148, 2057, 3468, 5256
Mph1103I ATGCAT 3 cut(s) 2443, 3895, 3936
MroI TCCGGA 1 cut(s) 3413
MroNI GCCGGC 1 cut(s) 896
MroXI GAANNNNTTC 1 cut(s) 4267
MscI TGGCCA 4 cut(s) 148, 2057, 3468, 5256
MseI TTAA 9 cut(s) 555, 867, 1281, 1590, 2390, 2678, 3968, 4254, 5544
MslI CAYNNNNRTG 2 cut(s) 3518, 5603
Msp20I TGGCCA 4 cut(s) 148, 2057, 3468, 5256
MspA1I CMGCKG 9 cut(s) 172, 452, 472, 809, 2576, 2810, 3263, 4001, 4013
MspI CCGG 5 cut(s) 897, 2533, 3060, 3414, 4679
MspR9I CCNGG 8 cut(s) 139, 1537, 3061, 4213, 4680, 5086, 5176, 5625
MunI CAATTG 6 cut(s) 510, 1908, 1920, 2508, 3744, 4895
Mva1269I GAATGC 2 cut(s) 2685, 4269
MvaI CCWGG 6 cut(s) 139, 1537, 4213, 5086, 5176, 5625
NaeI GCCGGC 1 cut(s) 898
NciI CCSGG 2 cut(s) 3061, 4680
NcoI CCATGG 2 cut(s) 2831, 5054
NdeI CATATG 2 cut(s) 245, 4747
NgoMIV GCCGGC 1 cut(s) 896
NmeAIII GCCGAG 2 cut(s) 4432, 5516
NmuCI GTSAC 6 cut(s) 17, 195, 1196, 1574, 1712, 5027
NsiI ATGCAT 3 cut(s) 2443, 3895, 3936
NspI RCATGY 5 cut(s) 36, 532, 846, 2193, 3824
NspV TTCGAA 2 cut(s) 858, 1255
OliI CACNNNNGTG 1 cut(s) 5603
PaeI GCATGC 2 cut(s) 36, 2193
PaeR7I CTCGAG 1 cut(s) 5481
PagI TCATGA 2 cut(s) 2868, 5437
PaqCI CACCTGC 3 cut(s) 2117, 4817, 4999
PceI AGGCCT 1 cut(s) 4417
PciSI GCTCTTC 2 cut(s) 324, 1640
PcsI WCGNNNNNNNCGW 1 cut(s) 5488
PctI GAATGC 2 cut(s) 2685, 4269
PdiI GCCGGC 1 cut(s) 898
PdmI GAANNNNTTC 1 cut(s) 4267
PflMI CCANNNNNTGG 2 cut(s) 144, 3254
PinAI ACCGGT 1 cut(s) 2532
PleI GAGTC 4 cut(s) 1733, 3316, 3931, 5087
PmaCI CACGTG 1 cut(s) 22
PmlI CACGTG 1 cut(s) 22
PpsI GAGTC 4 cut(s) 1733, 3316, 3931, 5087
Ppu21I YACGTR 1 cut(s) 22
PpuMI RGGWCCY 3 cut(s) 632, 2264, 2931
PshBI ATTAAT 2 cut(s) 1281, 4254
Psp124BI GAGCTC 1 cut(s) 374
Psp5II RGGWCCY 3 cut(s) 632, 2264, 2931
Psp6I CCWGG 6 cut(s) 137, 1535, 4211, 5084, 5174, 5623
PspCI CACGTG 1 cut(s) 22
PspFI CCCAGC 4 cut(s) 168, 2460, 3213, 4507
PspGI CCWGG 6 cut(s) 137, 1535, 4211, 5084, 5174, 5623
PspPPI RGGWCCY 3 cut(s) 632, 2264, 2931
PspXI VCTCGAGB 1 cut(s) 5481
PsrI GAACNNNNNNTAC 2 cut(s) 1363, 1395
PstNI CAGNNNCTG 5 cut(s) 812, 1338, 3462, 3608, 5411
PvuII CAGCTG 7 cut(s) 172, 452, 809, 2576, 2810, 4001, 4013
RseI CAYNNNNRTG 2 cut(s) 3518, 5603
SacI GAGCTC 1 cut(s) 374
SapI GCTCTTC 2 cut(s) 324, 1640
SaqAI TTAA 9 cut(s) 555, 867, 1281, 1590, 2390, 2678, 3968, 4254, 5544
ScaI AGTACT 1 cut(s) 1060
SchI GAGTC 4 cut(s) 1733, 3316, 3932, 5087
ScrFI CCNGG 8 cut(s) 139, 1537, 3061, 4213, 4680, 5086, 5176, 5625
SduI GDGCHC 5 cut(s) 374, 954, 2108, 2711, 4855
SfcI CTRYAG 4 cut(s) 121, 541, 3093, 3879
Sfr274I CTCGAG 1 cut(s) 5481
SfuI TTCGAA 2 cut(s) 858, 1255
SinI GGWCC 4 cut(s) 164, 632, 2264, 2931
SlaI CTCGAG 1 cut(s) 5481
SmiMI CAYNNNNRTG 2 cut(s) 3518, 5603
SmlI CTYRAG 6 cut(s) 373, 1135, 2134, 3326, 5398, 5481
SmoI CTYRAG 6 cut(s) 373, 1135, 2134, 3326, 5398, 5481
SpeI ACTAGT 4 cut(s) 1585, 3704, 4019, 4700
SphI GCATGC 2 cut(s) 36, 2193
SseBI AGGCCT 1 cut(s) 4417
SsiI CCGC 9 cut(s) 472, 1087, 1985, 2268, 3261, 3344, 3875, 4615, 5529
SspI AATATT 3 cut(s) 270, 3594, 4148
SstI GAGCTC 1 cut(s) 374
StuI AGGCCT 1 cut(s) 4417
StyD4I CCNGG 8 cut(s) 137, 1535, 3059, 4211, 4678, 5084, 5174, 5623
StyI CCWWGG 9 cut(s) 516, 721, 1174, 1444, 2379, 2831, 4229, 5054, 5676
TaiI ACGT 3 cut(s) 24, 787, 5378
TaqII GACCGA 1 cut(s) 2768
TatI WGTACW 7 cut(s) 235, 747, 1058, 2451, 2500, 2875, 4957
TauI GCSGC 1 cut(s) 3263
Tru1I TTAA 9 cut(s) 555, 867, 1281, 1590, 2390, 2678, 3968, 4254, 5544
Tru9I TTAA 9 cut(s) 555, 867, 1281, 1590, 2390, 2678, 3968, 4254, 5544
TseFI GTSAC 6 cut(s) 17, 195, 1196, 1574, 1712, 5027
Tsp45I GTSAC 6 cut(s) 17, 195, 1196, 1574, 1712, 5027
TspGWI ACGGA 6 cut(s) 49, 177, 1916, 2210, 4562, 4656
Van91I CCANNNNNTGG 2 cut(s) 144, 3254
VpaK11BI GGWCC 4 cut(s) 164, 632, 2264, 2931
VspI ATTAAT 2 cut(s) 1281, 4254
XagI CCTNNNNNAGG 2 cut(s) 1076, 4210
XbaI TCTAGA 3 cut(s) 2624, 3430, 4070
XceI RCATGY 5 cut(s) 36, 532, 846, 2193, 3824
XcmI CCANNNNNNNNNTGG 5 cut(s) 1869, 2485, 2514, 3536, 4001
XhoI CTCGAG 1 cut(s) 5481
XmaJI CCTAGG 1 cut(s) 4229
XmiI GTMKAC 1 cut(s) 4809
XmnI GAANNNNTTC 1 cut(s) 4267
ZrmI AGTACT 1 cut(s) 1060
Zsp2I ATGCAT 3 cut(s) 2443, 3895, 3936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.