Rw5G050130

Belongs to the peptidase C2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007783
Physical Location & Seq
Reverse (-)
86696093 .. 86711171
15079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G050130.1

Sequence Viewer

Length: 6417 bp
ATGGAAGGAGATGAGCGTCACGTGCTGCTCGCATGCGTCATTTCAGGAACCCTTTTCTCCGTTCTGGGTTCTGCTTCATTTTCTATACTCTGGCTTGTGAATTGGAGGCCGTGGAGGATCTACAGTTGGATATTCGCCAGGAAATGGCCAGATATCTTACACGGACCCCAGCTGGATATAGTATGTGGTTTTCTGTCACTCTCTGCATGGATTCTTGTCATTTCTCCAGTTCTTGTACTAATCATATGGGGAAGCTGGTTGGTTGTAATATTGGATCGACATATAATTGGTCTGGCTGTTATTATGGCTGGTACTGCTCTTCTACTGGCTTTCTATTCAATCATGCTTTGGTGGCGAACGCAATGGCAGAGCTCAAGGGCTGTTGCTATTCTTCTTCTTCTTGCTGTTGCCCTTCTCTGTGCCTATGAACTCTGTGCTGTATATGTTACAGCTGGTTCAAAAGCATCTCAGCGGTATTCGCCTTCTGGTTTCTTTTTTGGTGTATCAGCAATTGCCTTGGCAATCAACATGCTCTTTATCTGTAGAATGGTGTTTAATGGAAATGGCTTAGATGTCGATGAGTATGTGAGGAAGGCATACAAATTTGCTTATTCTGATTGTATAGAAGTAGGTCCTGTGGCCTGTTTACCAGAACCACCAGATCCGAATGAGTTATATCCTCGACAATCTAGCAGGGCTTCACATCTTGGGCTTCTTTACCTTGGCTCACTTGTAGTTCTTCTCGTGTACTCAATCCTATATGGACTTACTGCAAAGGACGCACGTTGGCTTGGAGCCATTACATCAGCTGCTGTGATAATTCTTGATTGGAACATGGGAGCATGTTTATATGGATTCGAACTCCTTAATAGTCGTGTAGCAGCATTATTTGTTGCCGGCACATCTCGAATTTTTCTCATTTGCTTCGGCGTTCACTACTGGTATTTGGGGCACTGCATTAGTTATGCAGTTGTAGCATCTGTTTTATTAGGTGCTTCTGTTTCTCGTCATTTATCTGTAACAAACCCATTGGCTGCAAGGAGAGATGCCTTACAGAGTACTGTTATTCGCCTCAGAGAGGGTTTCCGCAAAAAAGAACAGAACAGTTCATCAAGCTCATCTGAAGGTTGTGGCTCAAGCATGAAACGCAGTAGTAGTGTTGAAGCAGGTTGCCTTGGTAATGTTGTTGAAGCAAGTCACAGGAGCGCAACACACAGCACGGTTGATGCTAATAACTGGAGTAATGTTCTATTTCGAACTGCGAGTTCTCACGAGGGGATTAATAGTGATAAAAGCATAGATAGTGGGAGGCCAAGTATAGCATTGTGTAGCAGTTCCTGTCGTTCAGTAATCCAAGAGCCTGAAGTGGGAACTTCCTTTACTGACAAAAATTGCGATCAAAATAGCGCATTGGTGGTTTGTTCTAGCAGTGGTCTTGAAAGCCAAGGCTGTGAATCCAGTGCATCAAATTCTGCAAGTCAACAAACTTTAGATTTGAATTTAGCTTTTGCATTGCAAGAAAGGTTGAATGACCCCAGGATAACATCTATGCTAAAGAAGAGGGGAAGACAAAGTGACCTTGAACTAGTTAATCTTTTGCAGGATAAAGGACTGGATCCTAACTTTGTTATGATGTTGAAGGAGAAGAGCTTGGATCCAACCATTCTGGCATTACTTCAAAGAAGTAGTTTGGATGCTGATAGAGATCATCGTGACAATACAGATATCACCATAGCTGACTCAAACAGTGTGGATAATGGTTTACCCAATCAAATCTCTTTATCAGAAGAACTGAGGCTTCACGGGCTTGAAAAATGGCTCAAATTATCTAGGCTTGTTTTGCACCATGTAGTAGGTACCCCAGAGAGAGCATGGGTTCTCTTTAGTTTTGTTTTCATCCTCGAAACAATTGCTGTGGCAATTGTCCGTCCAAAGATAATCAAAATAATAAATGCGACACATCAACAGTTTGAATTTGGGTTTGCGGTGCTGCTGTTATCTCCTGTGGTCTGTTCAATCATGGCTTTTCTTCGATCTCTTCAAGCAGAAGAAATGGCCATGACCTCTAAGCCACGCAAGTATGGTTTTGTTGCTTGGCTGCTGAGCACCGTTGGATTATTGCTTTCCTTCTTGAGTAAATCATCAGTTCTTCTGGGATTGTCTTTGACTGTTCCCCTCATGGTGGCATGCCTTTCTGTTGCCATTCCTACATGGATCCGTAATGGCTACCAATTTTGGGTTCCTCAATTACATTGTGCAGGTCCTGCAGGGAACCATCAAATTCGGGGAACAAAAGAGGGTGTTATTCTTGTCTTCTGCACAACATTATTTACTGGATCTGTATTAGCTCTTGGTGCAATAGTGTCTGCAAAGCCCTTGGATGACTTAAATTACAAAGGATGGACGGGTGAACAGAAAAGCTTTACATCTCCTTATGCATCATCTGTGTACATTGGCTGGGCTATGGCTTCTGCCATTGCTTTAGTGGTTACTGGTGTACTGCCAATTGTTTCATGGTTTGCATCTTACCGGTTCTCCATGTTTTCTGCTGTGTGTGTCGGGATATTTACAGCTGTCCTCGTGTCATTTTGTGGTGCATCCTATATTGAGGTTGTGAAATCTAGAGATGACCAAGTTCCAACAAAGGGTGATTTCCTTGCTGCTTTACTTCCTTTAATTTGCATTCCAGCATTTCTGTCTCTGTGCTCGGGGTTGTATAAATGGAAGGATGATAATTGGAAGCTTTCTCGGGGTGTTTATATCTTTGTAACCATCGGTCTTCTACTTCTGCTCGGAGCAATATCAGCTGTTATAGTCGTAGTTACACCATGGACGGTTGGGGTATCATTTCTTTTAGTTCTTCTCATGATTGTACTGGCTATTGGTGCTATACATCATTGGGCCTCAAACAACTTTTATTTGACAAGGACCCAGACATTCTTTGTCTGTTTCCTCGCTTTTCTTTTGGCACTGGCTGCACTTCTTGTTGGATGGTTTGAAGATAAACCATTTGTCGGTGCTTCTGTCGGCTACTTTTTGTTTTTGTTTCTTCTTGCCGGGAGGGCATTGACTGTTCTTCTTTCACCTCCTATAGTTGTTTATTCTCCAAGAGTTCTCCCTGTTTATGTTTATGATGCTCATGCAGACTGTGCAAAGAATGTCAGTGCTGCATTTCTTGTGCTTTATGGGATTGCATTGGCAACTGAGGGCTGGGGTGTTGTTGCGAGTTTGAAAATTTATCCACCATTTGCTGGTGCCGCTGTATCAGCAATTACTCTTGTTGCTGCCTTTGGGTTTGCTTTCTCTCGTCCCTGCTTGACTCTCAAGATGATGGAAGATGCGGTACACTTTCTTAGTAAAGAAACCGTTGTTCAAGCTATTGCTAGATCTGCCACCAAGACCAGAAATGCTTTATCTGGAACTTATTCGGCTCCACAAAGGTCTGCCAGTTCAGCTCGTGATAGGGCAGGAAATTTTGTGCTTCCCAGAGCAGATGTCATGAAATTGAGAGATCGCTTAAGAAATGAGGAATATGGAAGGACTTTCCGCCATGAGCCAACAAGTAGTATTGATCACCGAAGAGAGATGTGTGCTCATGCACGAATATTGGCTTTGGAAGAGGCGATTGATACAGAATGGGTATATATGTGGGATAAATTTGGTGGTTATTTGCTTCTTTTGCTTGGTTTGACTGCTAAGGCAGAGCGAGTACAGGATGAGGTACGCTTGAGACTATTTCTTGATAGCATAGGATTTGCAGACTTAAGTGCCAAGAAAATAAAAAAATGGATGCCAGAGGATCGTAGGCAGTTTGAGATTATTCAGGAGAGTTACATAAGAGAAAAGGAGATGGAAGAGGAACTCTTAATGCAAAGACGTGAAGAGGAGGGGAAAGGCAAAGAGAGAAGAAAGGCTCTCTTGGAGAAGGAAGAACGCAAATGGAAGGAGATAGAAGCGTCTTTAATTTCCTCCATTCCTAATGCTGGCAGCAGGGAAGCTGCAGCTGTAGCAGCTGCAGTGCGTGCAGTAGGGGGTGATTCTGTTCTTGATGATTCTTTTGCTCGAGAGAGAGTCTCAAGCATTGCGCGTCGCATACGCACAGCTCAGTTAACTCGGCGAGCACTGCAGACAGGAATTTCCGGAGCTGTATGCGTTCTAGATGATGAGCCGACAACAAGTGGCAGGCACTGTGGCCAGATTGATTCTAGCATCTGTCAAAGTCAGAAGATCAGCTTTTCCATTGCAGTGATGATCCAACCTGTGTCTGGGCCTGTTTGCCTGTTAGGCACTGAGTTTCAAAAGAAAGTTTGTTGGGAAATATTGGTCGCCGGTTCTGAGCAAGGAATTGAAGCTGGACAAGTTGGGCTTAGATTGATCACTAAAGGCGACAGACAAACTACTGTTGCAAAGGAGTGGAGTATTAGTGCAACTAGTATTGCAGATGGAAGGTGGCATTTGGTAACAATGACAATTGATGCTGATTTAGGTGAGGCAACTTGCTATCTCGATGGTGGTTTTGATGGCTACCAGACTGGTTTACCATTGCATGTGGGTAATACTATTTGGGAGCAAGGAACAGAAGTTTGGGTAGGTGTTAGACCGCCTACAGATATGGATGCATTTGGGAGGTCAGACAGTGAAGGGGCTGAGTCCAAGATGCATATTATGGATGTGTTTCTATGGGGAAGGTGCTTAACTGAGGATGATATTGCTGCTCTCCATACAGCTGTTGGGTCAGCTGATACTAGTATGATAGATTTTCCTGAAGATGCTTGGCAGTGGGCAGATTCACCTTCTAGACTTGATGAATGGGATAGTGATCCTGCCGATGTAGATCTATATGATAGGGATGAAGTTGATTGGGATGGGCAATATTCTAGTGGAAGGAAAAGAAGATCAGAGCGTGATGGAGTTCTGGTTGATTTGGATTCCTTTGCCAGGAGGTTTAGGAAACCTAGGATGGAAACGCAAGAAGAAATTAATCAACGAATGCTTTCTGTTGAATTGGCCGTCAAAGAAGCTCTCTGTGCAAGAGGAGAAACAAATTTTACTGATCAGGAGTTTCCTCCAAATGATCAATCACTATTTGTAGATTCGGAAAATCCCCCTTCAAAGTTGCAGGTCGTTTCAGAATGGATGAGGCCTGCTGATATTGTAAAAGAGAGCAGGCTTGCTACTCGGCCGTGCTTATTTTCTGGCACTGTGAATCCTTCTGATGTTTGTCAGGGTCGGCTGGGGGATTGTTGGTTTTTGAGTGCTGTTGCTGTTCTGACGGAAGTTTCTCGAATATCAGAAGTTATTATTACTCCAGAGTATAATGAGGAAGGAATCTACACTGTCCGCTTCTGTATTCAGGGTGAGTGGGTTCCTGTTGTTGTTGATGATTGGATTCCGTGTGAATCACCGGGTAAACCAGCATTTGCTACTAGTAGAAAAGGTAACGAGTTGTGGGTCTCTTTACTGGAGAAGGCATATGCCAAATTGCATGGCTCCTATGAAGCATTAGAAGGTGGACTTGTCCAAGATGCTCTTGTAGACCTCACTGGAGGTGCAGGTGAAGAAATTGATATGAGGAGTGCTCAGGCCCAAATTGATCTTGCGAGTGGTCGATTATGGTCTCAATTGTTGCGCTTCAAACAAGAGGGATTTCTTCTGGGTGCTGGAAGTCCATCTGGTTCAGATGTACATATTTCCTCCAGTGGCATTGTGCAAGGTCATGCTTACTCGTTATTGCAGGTGAGAGAGGTGGATGGTCACAAGCTTATTCAGATTCGCAATCCATGGGCCAATGAAGTTGAGTGGAATGGCCCCTGGTCTGACTCATCGCCTGAATGGACTGATAGGATGAAGCATAAGCTGAAGCATATTCCACAGTCAAAAGATGGCATATTCTGGATGTCCTGGCAAGACTTCCAAATTCATTTTCGATCAATATATGTTTGCCGTATCTATCCCCCTGAGATGCGCTACTCTGTTCATGGCCAATGGCGAAGTTATAGTGCTGGTGGCTGCCAAGACTATGAGACATGGCATCAAAATCCACAATTCCGATTGAGGGCCACAGGGCCAGATGCCTCATTTCCAATTCATGTATTCATTACGTTGACACAGGGTGTGAGCTTCTCAAGAACAGTTGCTGGTTTCAGAAACTATCAATCCAGTCATGATTCAATGATGTTCTATATTGGGATGAGGATACTCAAAACTCGAGGTCGCCGAGCTGCTTACAATATATACCTACATGAATCAGTCGGCGGGACAGATTATGTTAATTCACGAGAAATATCTTGTGAAATGGTTTTGGATCCAGATCCAAAAGGTTACACAATAGTGCCGACAACTATACACCCTGGCGAAGAAGCTCCATTTGTTCTTTCTGTTTTCACAAAAGCATCAATAACCTTGGAAGCCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001558 GO:0001708 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0004198 GO:0005198 GO:0005488 GO:0005509 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0005829 GO:0005886 GO:0005887 GO:0006275 GO:0006355 GO:0006508 GO:0006807 GO:0006873 GO:0006874 GO:0006875 GO:0006950 GO:0006970 GO:0006996 GO:0007010 GO:0007204 GO:0007275 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0008307 GO:0009628 GO:0009651 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009934 GO:0009966 GO:0009987 GO:0010008 GO:0010035 GO:0010038 GO:0010154 GO:0010468 GO:0010522 GO:0010524 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010646 GO:0010927 GO:0010941 GO:0010959 GO:0012505 GO:0014717 GO:0014718 GO:0014823 GO:0014850 GO:0016020 GO:0016021 GO:0016043 GO:0016787 GO:0019219 GO:0019222 GO:0019538 GO:0019725 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0030003 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030036 GO:0030154 GO:0030162 GO:0030234 GO:0030239 GO:0030315 GO:0031032 GO:0031090 GO:0031224 GO:0031226 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031402 GO:0031410 GO:0031420 GO:0031432 GO:0031674 GO:0031982 GO:0031984 GO:0032101 GO:0032268 GO:0032269 GO:0032270 GO:0032501 GO:0032502 GO:0032875 GO:0032877 GO:0032879 GO:0032947 GO:0032989 GO:0032991 GO:0033036 GO:0033233 GO:0033234 GO:0033554 GO:0034613 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0040008 GO:0042127 GO:0042175 GO:0042221 GO:0042383 GO:0042592 GO:0042692 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043269 GO:0043270 GO:0043292 GO:0043415 GO:0043416 GO:0043933 GO:0044085 GO:0044093 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044449 GO:0044459 GO:0044464 GO:0045165 GO:0045214 GO:0045740 GO:0045787 GO:0045862 GO:0045892 GO:0045893 GO:0045927 GO:0045934 GO:0045935 GO:0046872 GO:0048316 GO:0048468 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048583 GO:0048584 GO:0048608 GO:0048638 GO:0048639 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050801 GO:0050865 GO:0050867 GO:0050896 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051146 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051239 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051279 GO:0051281 GO:0051282 GO:0051480 GO:0051592 GO:0051641 GO:0051716 GO:0051726 GO:0051924 GO:0051928 GO:0055001 GO:0055002 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055103 GO:0060255 GO:0060341 GO:0060548 GO:0061041 GO:0061061 GO:0061458 GO:0065003 GO:0065007 GO:0065008 GO:0065009 GO:0070011 GO:0070727 GO:0070887 GO:0070925 GO:0071214 GO:0071241 GO:0071248 GO:0071277 GO:0071470 GO:0071472 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072657 GO:0080050 GO:0080090 GO:0080134 GO:0090068 GO:0090303 GO:0090329 GO:0090392 GO:0090627 GO:0090628 GO:0097264 GO:0097435 GO:0097708 GO:0098588 GO:0098771 GO:0098772 GO:0098805 GO:0098827 GO:0099080 GO:0099081 GO:0099512 GO:0104004 GO:0140096 GO:1901564 GO:1902531 GO:1902679 GO:1902680 GO:1903034 GO:1903036 GO:1903169 GO:1903320 GO:1903321 GO:1903506 GO:1903507 GO:1903508 GO:1904062 GO:1904064 GO:1904427 GO:2000011 GO:2000014 GO:2000024 GO:2000026 GO:2000105 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calpain_III Peptidase_C2

Protein Analysis

2138

Amino Acids

236.74

Weight (kDa)

6.08

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C2 PF00648 1683 - 1982 4.5e-89 Calpain family cysteine protease
Calpain_III PF01067 1993 - 2128 1.1e-15 Calpain large subunit, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 5543, 5725
Acc36I ACCTGC 5 cut(s) 1157, 2249, 5110, 5543, 5725
Acc65I GGTACC 1 cut(s) 1856
AccB1I GGYRCC 2 cut(s) 1856, 3254
AccB7I CCANNNNNTGG 2 cut(s) 144, 3251
AccI GTMKAC 1 cut(s) 5535
AccII CGCG 1 cut(s) 4087
AccIII TCCGGA 1 cut(s) 4139
AciI CCGC 9 cut(s) 472, 1087, 1985, 3258, 3341, 3547, 4601, 5341, 6255
AcoI YGGCCR 6 cut(s) 146, 2055, 4192, 5007, 5180, 5980
AcuI CTGAAG 4 cut(s) 1143, 1383, 4785, 5879
AcvI CACGTG 1 cut(s) 22
AdeI CACNNNGTG 1 cut(s) 6113
AflII CTTAAG 2 cut(s) 3517, 3763
AgeI ACCGGT 1 cut(s) 2529
AhlI ACTAGT 4 cut(s) 1585, 4430, 4745, 5426
AjiI CACGTC 1 cut(s) 3878
AjnI CCWGG 6 cut(s) 137, 1535, 4937, 5810, 5900, 6349
AjuI GAANNNNNNNTTGG 6 cut(s) 2223, 2255, 4567, 4599, 5907, 5939
AleI CACNNNNGTG 1 cut(s) 6329
Alw21I GWGCWC 6 cut(s) 374, 2108, 2708, 3595, 4123, 5581
Alw26I GTCTC 6 cut(s) 2703, 3724, 4078, 5458, 5622, 6017
AlwNI CAGNNNCTG 5 cut(s) 812, 1338, 2264, 4188, 6137
Ama87I CYCGRG 4 cut(s) 2707, 2748, 4062, 6207
Aor13HI TCCGGA 1 cut(s) 4139
AseI ATTAAT 2 cut(s) 1281, 4980
AsiGI ACCGGT 1 cut(s) 2529
Asp700I GAANNNNTTC 2 cut(s) 3424, 4993
Asp718I GGTACC 1 cut(s) 1856
AspA2I CCTAGG 1 cut(s) 4955
AspLEI GCGC 5 cut(s) 1208, 1409, 4087, 5631, 5967
AsuC2I CCSGG 2 cut(s) 3058, 5406
AsuII TTCGAA 2 cut(s) 858, 1255
AvaI CYCGRG 4 cut(s) 2707, 2748, 4062, 6207
AvaII GGWCC 4 cut(s) 164, 632, 2261, 2928
AvrII CCTAGG 1 cut(s) 4955
BaeGI GKGCMC 1 cut(s) 954
BalI TGGCCA 4 cut(s) 148, 2057, 4194, 5982
BamHI GGATCC 4 cut(s) 1615, 1654, 2214, 6304
BanI GGYRCC 2 cut(s) 1856, 3254
BanII GRGCYC 1 cut(s) 374
BarI GAAGNNNNNNTAC 2 cut(s) 3327, 3359
BauI CACGAG 5 cut(s) 743, 1271, 2579, 3456, 6276
BbrPI CACGTG 1 cut(s) 22
BbsI GAAGAC 3 cut(s) 1573, 2305, 2771
Bbv12I GWGCWC 6 cut(s) 374, 2108, 2708, 3595, 4123, 5581
BceAI ACGGC 4 cut(s) 94, 4994, 5167, 5928
BcgI CGANNNNNNTGC 2 cut(s) 1892, 1926
BciT130I CCWGG 6 cut(s) 139, 1537, 4939, 5812, 5902, 6351
BciVI GTATCC 1 cut(s) 6189
BclI TGATCA 4 cut(s) 3571, 4374, 5053, 5074
BcnI CCSGG 2 cut(s) 3058, 5406
BcoDI GTCTC 6 cut(s) 2703, 3724, 4078, 5458, 5622, 6017
BcuI ACTAGT 4 cut(s) 1585, 4430, 4745, 5426
BfmI CTRYAG 9 cut(s) 121, 541, 2265, 3090, 3999, 4005, 4014, 4124, 4605
BfrI CTTAAG 2 cut(s) 3517, 3763
BfuAI ACCTGC 5 cut(s) 1157, 2249, 5110, 5543, 5725
BfuI GTATCC 1 cut(s) 6189
BglI GCCNNNNNGGC 2 cut(s) 3062, 4284
BglII AGATCT 2 cut(s) 3386, 4834
BlnI CCTAGG 1 cut(s) 4955
BlpI GCTNAGC 1 cut(s) 2102
BmcAI AGTACT 1 cut(s) 1060
Bme1390I CCNGG 8 cut(s) 139, 1537, 3058, 4939, 5406, 5812, 5902, 6351
Bme18I GGWCC 4 cut(s) 164, 632, 2261, 2928
BmeT110I CYCGRG 4 cut(s) 2707, 2748, 4062, 6207
BmgBI CACGTC 1 cut(s) 3878
BmrFI CCNGG 8 cut(s) 139, 1537, 3058, 4939, 5406, 5812, 5902, 6351
BpiI GAAGAC 3 cut(s) 1573, 2305, 2771
BplI GAGNNNNNCTC 4 cut(s) 4058, 4090, 5563, 5595
BpmI CTGGAG 6 cut(s) 210, 1258, 5292, 5483, 5565, 5680
Bpu10I CCTNAGC 2 cut(s) 3696, 5580
Bpu1102I GCTNAGC 1 cut(s) 2102
Bpu14I TTCGAA 2 cut(s) 858, 1255
BpuEI CTTGAG 7 cut(s) 358, 1120, 2152, 3308, 3748, 4060, 6109
BpuMI CCSGG 2 cut(s) 3058, 5406
BsaAI YACGTR 1 cut(s) 22
BsaBI GATNNNNATC 4 cut(s) 1704, 4818, 4833, 6309
BsaI GGTCTC 2 cut(s) 5458, 5622
BsaWI WCCGGW 2 cut(s) 2529, 4139
BsaXI ACNNNNNCTCC 8 cut(s) 1300, 1330, 2519, 2549, 4408, 4438, 4902, 4932
Bse118I RCCGGY 3 cut(s) 896, 2529, 4328
Bse3DI GCAATG 6 cut(s) 368, 1511, 2475, 4080, 4239, 4541
Bse8I GATNNNNATC 4 cut(s) 1704, 4818, 4833, 6309
BseAI TCCGGA 1 cut(s) 4139
BseBI CCWGG 6 cut(s) 139, 1537, 4939, 5812, 5902, 6351
BseJI GATNNNNATC 4 cut(s) 1704, 4818, 4833, 6309
BseMI GCAATG 6 cut(s) 368, 1511, 2475, 4080, 4239, 4541
BseRI GAGGAG 3 cut(s) 3899, 5049, 5587
BseSI GKGCMC 1 cut(s) 954
BseX3I CGGCCG 1 cut(s) 5180
BseYI CCCAGC 4 cut(s) 168, 2457, 3210, 5233
BsgI GTGCAG 5 cut(s) 2277, 2302, 2961, 4044, 5571
Bsh1236I CGCG 1 cut(s) 4087
Bsh1285I CGRYCG 1 cut(s) 5183
BshNI GGYRCC 2 cut(s) 1856, 3254
BshTI ACCGGT 1 cut(s) 2529
BsiEI CGRYCG 1 cut(s) 5183
BsiHKAI GWGCWC 6 cut(s) 374, 2108, 2708, 3595, 4123, 5581
BsiHKCI CYCGRG 4 cut(s) 2707, 2748, 4062, 6207
BsiSI CCGG 6 cut(s) 897, 2530, 3057, 4140, 4329, 5405
BslFI GGGAC 2 cut(s) 3294, 6271
BsmAI GTCTC 6 cut(s) 2703, 3724, 4078, 5458, 5622, 6017
BsmFI GGGAC 2 cut(s) 3294, 6271
BsmI GAATGC 2 cut(s) 2682, 4995
Bso31I GGTCTC 2 cut(s) 5458, 5622
BsoBI CYCGRG 4 cut(s) 2707, 2748, 4062, 6207
Bsp119I TTCGAA 2 cut(s) 858, 1255
Bsp1286I GDGCHC 7 cut(s) 374, 954, 2108, 2708, 3595, 4123, 5581
Bsp13I TCCGGA 1 cut(s) 4139
Bsp1407I TGTACA 2 cut(s) 2448, 5683
Bsp1720I GCTNAGC 1 cut(s) 2102
Bsp19I CCATGG 2 cut(s) 2828, 5780
BspACI CCGC 9 cut(s) 472, 1087, 1985, 3258, 3341, 3547, 4601, 5341, 6255
BspEI TCCGGA 1 cut(s) 4139
BspFNI CGCG 1 cut(s) 4087
BspHI TCATGA 3 cut(s) 2865, 3498, 6163
BspMAI CTGCAG 4 cut(s) 2269, 4003, 4018, 4128
BspMI ACCTGC 5 cut(s) 1157, 2249, 5110, 5543, 5725
BspQI GCTCTTC 2 cut(s) 324, 1640
BspT104I TTCGAA 2 cut(s) 858, 1255
BspT107I GGYRCC 2 cut(s) 1856, 3254
BspTI CTTAAG 2 cut(s) 3517, 3763
BspTNI GGTCTC 2 cut(s) 5458, 5622
BsrDI GCAATG 6 cut(s) 368, 1511, 2475, 4080, 4239, 4541
BsrFI RCCGGY 3 cut(s) 896, 2529, 4328
BsrGI TGTACA 2 cut(s) 2448, 5683
BssAI RCCGGY 3 cut(s) 896, 2529, 4328
BssSI CACGAG 5 cut(s) 743, 1271, 2579, 3456, 6276
BssT1I CCWWGG 9 cut(s) 516, 721, 1174, 1444, 2376, 2828, 4955, 5780, 6402
Bst2BI CACGAG 5 cut(s) 743, 1271, 2579, 3456, 6276
Bst2UI CCWGG 6 cut(s) 139, 1537, 4939, 5812, 5902, 6351
Bst6I CTCTTC 8 cut(s) 324, 1553, 1640, 2043, 3574, 3612, 3849, 3876
BstAFI CTTAAG 2 cut(s) 3517, 3763
BstAPI GCANNNNNTGC 4 cut(s) 2264, 2975, 3149, 4022
BstAUI TGTACA 2 cut(s) 2448, 5683
BstBAI YACGTR 1 cut(s) 22
BstBI TTCGAA 2 cut(s) 858, 1255
BstDSI CCRYGG 3 cut(s) 110, 2828, 5780
BstENI CCTNNNNNAGG 2 cut(s) 1076, 4936
BstFNI CGCG 1 cut(s) 4087
BstHHI GCGC 5 cut(s) 1208, 1409, 4087, 5631, 5967
BstMAI GTCTC 6 cut(s) 2703, 3724, 4078, 5458, 5622, 6017
BstMCI CGRYCG 1 cut(s) 5183
BstNI CCWGG 6 cut(s) 139, 1537, 4939, 5812, 5902, 6351
BstNSI RCATGY 5 cut(s) 36, 532, 846, 2190, 4550
BstSCI CCNGG 8 cut(s) 137, 1535, 3056, 4937, 5404, 5810, 5900, 6349
BstSFI CTRYAG 9 cut(s) 121, 541, 2265, 3090, 3999, 4005, 4014, 4124, 4605
BstSLI GKGCMC 1 cut(s) 954
BstUI CGCG 1 cut(s) 4087
BstV2I GAAGAC 3 cut(s) 1573, 2305, 2771
BstZI CGGCCG 1 cut(s) 5180
BsuI GTATCC 1 cut(s) 6189
BtgI CCRYGG 3 cut(s) 110, 2828, 5780
BtgZI GCGATG 1 cut(s) 5808
BtrI CACGTC 1 cut(s) 3878
BtsI GCAGTG 6 cut(s) 952, 1435, 4023, 4121, 4251, 4784
BveI ACCTGC 5 cut(s) 1157, 2249, 5110, 5543, 5725
CaiI CAGNNNCTG 5 cut(s) 812, 1338, 2264, 4188, 6137
CciI TCATGA 3 cut(s) 2865, 3498, 6163
CfoI GCGC 5 cut(s) 1208, 1409, 4087, 5631, 5967
Cfr10I RCCGGY 3 cut(s) 896, 2529, 4328
CseI GACGC 5 cut(s) 5, 25, 788, 3945, 4076
CspAI ACCGGT 1 cut(s) 2529
CspCI CAANNNNNGTGG 4 cut(s) 1731, 1766, 1896, 1931
DraIII CACNNNGTG 1 cut(s) 6113
EaeI YGGCCR 6 cut(s) 146, 2055, 4192, 5007, 5180, 5980
EagI CGGCCG 1 cut(s) 5180
Eam1104I CTCTTC 8 cut(s) 324, 1553, 1640, 2043, 3574, 3612, 3849, 3876
EarI CTCTTC 8 cut(s) 324, 1553, 1640, 2043, 3574, 3612, 3849, 3876
EciI GGCGGA 1 cut(s) 3536
Ecl136II GAGCTC 1 cut(s) 372
EclXI CGGCCG 1 cut(s) 5180
Eco130I CCWWGG 9 cut(s) 516, 721, 1174, 1444, 2376, 2828, 4955, 5780, 6402
Eco147I AGGCCT 1 cut(s) 5143
Eco24I GRGCYC 1 cut(s) 374
Eco31I GGTCTC 2 cut(s) 5458, 5622
Eco32I GATATC 2 cut(s) 154, 1726
Eco47I GGWCC 4 cut(s) 164, 632, 2261, 2928
Eco52I CGGCCG 1 cut(s) 5180
Eco53kI GAGCTC 1 cut(s) 372
Eco57I CTGAAG 4 cut(s) 1143, 1383, 4785, 5879
Eco72I CACGTG 1 cut(s) 22
Eco88I CYCGRG 4 cut(s) 2707, 2748, 4062, 6207
EcoICRI GAGCTC 1 cut(s) 372
EcoNI CCTNNNNNAGG 2 cut(s) 1076, 4936
EcoO109I RGGNCCY 3 cut(s) 632, 2261, 2928
EcoRII CCWGG 6 cut(s) 137, 1535, 4937, 5810, 5900, 6349
EcoRV GATATC 2 cut(s) 154, 1726
EcoT14I CCWWGG 9 cut(s) 516, 721, 1174, 1444, 2376, 2828, 4955, 5780, 6402
EcoT22I ATGCAT 3 cut(s) 2440, 4621, 4662
EcoT38I GRGCYC 1 cut(s) 374
ErhI CCWWGG 9 cut(s) 516, 721, 1174, 1444, 2376, 2828, 4955, 5780, 6402
FaqI GGGAC 2 cut(s) 3294, 6271
FauI CCCGC 1 cut(s) 6248
FauNDI CATATG 2 cut(s) 245, 5473
FbaI TGATCA 4 cut(s) 3571, 4374, 5053, 5074
FblI GTMKAC 1 cut(s) 5535
FriOI GRGCYC 1 cut(s) 374
GlaI GCGC 5 cut(s) 1207, 1408, 4086, 5630, 5966
GsaI CCCAGC 4 cut(s) 172, 2461, 3214, 5237
GsuI CTGGAG 6 cut(s) 210, 1258, 5292, 5483, 5565, 5680
HapII CCGG 6 cut(s) 897, 2530, 3057, 4140, 4329, 5405
HgaI GACGC 5 cut(s) 5, 25, 788, 3945, 4076
HhaI GCGC 5 cut(s) 1208, 1409, 4087, 5631, 5967
Hin6I GCGC 5 cut(s) 1206, 1407, 4085, 5629, 5965
HinP1I GCGC 5 cut(s) 1206, 1407, 4085, 5629, 5965
HincII GTYRAC 3 cut(s) 1481, 4110, 6105
HindII GTYRAC 3 cut(s) 1481, 4110, 6105
HindIII AAGCTT 3 cut(s) 2419, 2741, 5759
HpaI GTTAAC 1 cut(s) 4110
HpaII CCGG 6 cut(s) 897, 2530, 3057, 4140, 4329, 5405
Hpy99I CGWCG 1 cut(s) 4092
HpyCH4IV ACGT 4 cut(s) 21, 784, 3877, 6101
HpySE526I ACGT 4 cut(s) 21, 784, 3877, 6101
HspAI GCGC 5 cut(s) 1206, 1407, 4085, 5629, 5965
Kpn2I TCCGGA 1 cut(s) 4139
KpnI GGTACC 1 cut(s) 1860
KroI GCCGGC 1 cut(s) 896
KroNI GCCGGC 1 cut(s) 898
Ksp22I TGATCA 4 cut(s) 3571, 4374, 5053, 5074
KspAI GTTAAC 1 cut(s) 4110
LguI GCTCTTC 2 cut(s) 324, 1640
LmnI GCTCC 9 cut(s) 794, 839, 1203, 2795, 3436, 4142, 4567, 5495, 6367
MaeII ACGT 4 cut(s) 21, 784, 3877, 6101
MfeI CAATTG 6 cut(s) 510, 1908, 1920, 2505, 4470, 5621
MhlI GDGCHC 7 cut(s) 374, 954, 2108, 2708, 3595, 4123, 5581
MlsI TGGCCA 4 cut(s) 148, 2057, 4194, 5982
MluNI TGGCCA 4 cut(s) 148, 2057, 4194, 5982
MlyI GAGTC 5 cut(s) 1733, 3313, 4080, 4658, 5813
MmeI TCCRAC 6 cut(s) 107, 1682, 2092, 2663, 2968, 4279
Mox20I TGGCCA 4 cut(s) 148, 2057, 4194, 5982
Mph1103I ATGCAT 3 cut(s) 2440, 4621, 4662
MroI TCCGGA 1 cut(s) 4139
MroNI GCCGGC 1 cut(s) 896
MroXI GAANNNNTTC 2 cut(s) 3424, 4993
MscI TGGCCA 4 cut(s) 148, 2057, 4194, 5982
MslI CAYNNNNRTG 2 cut(s) 4244, 6329
Msp20I TGGCCA 4 cut(s) 148, 2057, 4194, 5982
MspCI CTTAAG 2 cut(s) 3517, 3763
MspI CCGG 6 cut(s) 897, 2530, 3057, 4140, 4329, 5405
MspR9I CCNGG 8 cut(s) 139, 1537, 3058, 4939, 5406, 5812, 5902, 6351
MunI CAATTG 6 cut(s) 510, 1908, 1920, 2505, 4470, 5621
Mva1269I GAATGC 2 cut(s) 2682, 4995
MvaI CCWGG 6 cut(s) 139, 1537, 4939, 5812, 5902, 6351
MvnI CGCG 1 cut(s) 4087
NaeI GCCGGC 1 cut(s) 898
NciI CCSGG 2 cut(s) 3058, 5406
NcoI CCATGG 2 cut(s) 2828, 5780
NdeI CATATG 2 cut(s) 245, 5473
NgoMIV GCCGGC 1 cut(s) 896
NmeAIII GCCGAG 3 cut(s) 4093, 5158, 6242
NmuCI GTSAC 6 cut(s) 17, 195, 1196, 1574, 1712, 5753
NsiI ATGCAT 3 cut(s) 2440, 4621, 4662
NspI RCATGY 5 cut(s) 36, 532, 846, 2190, 4550
NspV TTCGAA 2 cut(s) 858, 1255
OliI CACNNNNGTG 1 cut(s) 6329
PaeI GCATGC 2 cut(s) 36, 2190
PaeR7I CTCGAG 2 cut(s) 4062, 6207
PagI TCATGA 3 cut(s) 2865, 3498, 6163
PaqCI CACCTGC 2 cut(s) 5543, 5725
PceI AGGCCT 1 cut(s) 5143
PciSI GCTCTTC 2 cut(s) 324, 1640
PcsI WCGNNNNNNNCGW 1 cut(s) 6214
PctI GAATGC 2 cut(s) 2682, 4995
PdiI GCCGGC 1 cut(s) 898
PdmI GAANNNNTTC 2 cut(s) 3424, 4993
PflMI CCANNNNNTGG 2 cut(s) 144, 3251
PinAI ACCGGT 1 cut(s) 2529
PleI GAGTC 5 cut(s) 1733, 3313, 4079, 4657, 5813
PmaCI CACGTG 1 cut(s) 22
PmlI CACGTG 1 cut(s) 22
PpsI GAGTC 5 cut(s) 1733, 3313, 4079, 4657, 5813
Ppu21I YACGTR 1 cut(s) 22
PpuMI RGGWCCY 3 cut(s) 632, 2261, 2928
PshBI ATTAAT 2 cut(s) 1281, 4980
Psp124BI GAGCTC 1 cut(s) 374
Psp5II RGGWCCY 3 cut(s) 632, 2261, 2928
Psp6I CCWGG 6 cut(s) 137, 1535, 4937, 5810, 5900, 6349
PspCI CACGTG 1 cut(s) 22
PspFI CCCAGC 4 cut(s) 168, 2457, 3210, 5233
PspGI CCWGG 6 cut(s) 137, 1535, 4937, 5810, 5900, 6349
PspPPI RGGWCCY 3 cut(s) 632, 2261, 2928
PspXI VCTCGAGB 1 cut(s) 6207
PsrI GAACNNNNNNTAC 2 cut(s) 1363, 1395
PstI CTGCAG 4 cut(s) 2269, 4003, 4018, 4128
PstNI CAGNNNCTG 5 cut(s) 812, 1338, 2264, 4188, 6137
PvuII CAGCTG 9 cut(s) 172, 452, 809, 2573, 2807, 4004, 4013, 4727, 4739
RseI CAYNNNNRTG 2 cut(s) 4244, 6329
SacI GAGCTC 1 cut(s) 374
SapI GCTCTTC 2 cut(s) 324, 1640
SbfI CCTGCAGG 1 cut(s) 2269
ScaI AGTACT 1 cut(s) 1060
SchI GAGTC 5 cut(s) 1733, 3313, 4080, 4658, 5813
ScrFI CCNGG 8 cut(s) 139, 1537, 3058, 4939, 5406, 5812, 5902, 6351
SdaI CCTGCAGG 1 cut(s) 2269
SduI GDGCHC 7 cut(s) 374, 954, 2108, 2708, 3595, 4123, 5581
SfcI CTRYAG 9 cut(s) 121, 541, 2265, 3090, 3999, 4005, 4014, 4124, 4605
Sfr274I CTCGAG 2 cut(s) 4062, 6207
SfuI TTCGAA 2 cut(s) 858, 1255
SinI GGWCC 4 cut(s) 164, 632, 2261, 2928
SlaI CTCGAG 2 cut(s) 4062, 6207
SmiMI CAYNNNNRTG 2 cut(s) 4244, 6329
SpeI ACTAGT 4 cut(s) 1585, 4430, 4745, 5426
SphI GCATGC 2 cut(s) 36, 2190
Sse8387I CCTGCAGG 1 cut(s) 2269
SseBI AGGCCT 1 cut(s) 5143
SsiI CCGC 9 cut(s) 472, 1087, 1985, 3258, 3341, 3547, 4601, 5341, 6255
SspI AATATT 4 cut(s) 270, 3606, 4320, 4874
SstI GAGCTC 1 cut(s) 374
StuI AGGCCT 1 cut(s) 5143
StyD4I CCNGG 8 cut(s) 137, 1535, 3056, 4937, 5404, 5810, 5900, 6349
StyI CCWWGG 9 cut(s) 516, 721, 1174, 1444, 2376, 2828, 4955, 5780, 6402
TaiI ACGT 4 cut(s) 24, 787, 3880, 6104
TaqII GACCGA 1 cut(s) 2765
TatI WGTACW 8 cut(s) 235, 747, 1058, 2448, 2497, 2872, 3709, 5683
TauI GCSGC 1 cut(s) 3260
TseFI GTSAC 6 cut(s) 17, 195, 1196, 1574, 1712, 5753
Tsp45I GTSAC 6 cut(s) 17, 195, 1196, 1574, 1712, 5753
TspGWI ACGGA 6 cut(s) 49, 177, 1916, 2207, 5288, 5382
Van91I CCANNNNNTGG 2 cut(s) 144, 3251
Vha464I CTTAAG 2 cut(s) 3517, 3763
VpaK11BI GGWCC 4 cut(s) 164, 632, 2261, 2928
VspI ATTAAT 2 cut(s) 1281, 4980
XagI CCTNNNNNAGG 2 cut(s) 1076, 4936
XbaI TCTAGA 3 cut(s) 2621, 4156, 4796
XceI RCATGY 5 cut(s) 36, 532, 846, 2190, 4550
XcmI CCANNNNNNNNNTGG 5 cut(s) 1869, 2482, 2511, 4262, 4727
XhoI CTCGAG 2 cut(s) 4062, 6207
XmaJI CCTAGG 1 cut(s) 4955
XmiI GTMKAC 1 cut(s) 5535
XmnI GAANNNNTTC 2 cut(s) 3424, 4993
ZrmI AGTACT 1 cut(s) 1060
Zsp2I ATGCAT 3 cut(s) 2440, 4621, 4662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.