Rh5DG572900

Belongs to the peptidase C2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007783
Physical Location & Seq
Reverse (-)
88012933 .. 88028150
15218 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG572900.1

Sequence Viewer

Length: 6483 bp
ATGGAAGGAGATGAGCGTCACGTGCTGCTCGCATGCGTCATTTCAGGAACCCTTTTCTCCGTTCTGGGTTCTGCTTCGTTTTCTATACTCTGGCTTGTGAATTGGAGGCCGTGGAGGATCTACAGTTGGATATTCGCCAGGAAATGGCCAGATATCTTACACGGACCCCAGCTGGATATAGTATGTGGTTTTCTGTCACTCTCTGCATGGATTCTTGTCATTTCTCCAGTTCTTGTACTAATCATATGGGGAAGCTGGTTGGTTGTAATATTGGATCGACATATAATTGGTCTGGCTGTTATTATGGCTGGTACTGCTCTTCTACTGGCTTTCTATTCAATCATGCTTTGGTGGCGAACGCAATGGCAGAGCTCAAGGGCTGTTGCTATTCTTCTTCTTCTTGCTGTTGCCCTTCTCTGTGCCTATGAACTCTGTGCTGTATATGTTACAGCTGGTTCAAAAGCATCTCAGCGGTATTCGCCTTCTGGTTTCTTTTTTGGTGTATCAGCAATTGCCTTGGCAATCAACATGCTCTTTATCTGTAGAATGGTGTTTAATGGAAATGGCTTAGATGTCGATGAGTATGTGAGGAAGGCATACAAATTTGCTTATTCTGATTGTATAGAAGTAGGTCCTGTGGCCTGTTTACCAGAACCACCAGATCCGAATGAGTTATATCCTCGACAATCTAGCAGGGCTTCACATCTTGGGCTTCTTTACCTTGGCTCACTTGTAGTTCTTCTCGTGTACTCAATCCTATATGGACTTACTGCAAAGGACGCACGTTGGCTTGGAGCCATTACATCAGCTGCTGTGATAATTCTTGATTGGAACATGGGAGCATGTTTATATGGATTCGAACTCCTTAATAGTCGTGTAGCAGCATTATTTGTTGCCGGCACATCTCGAATTTTTCTCATTTGCTTCGGCGTTCACTACTGGTATTTGGGGCACTGCATTAGTTATGCAGTTGTAGCATCTGTTTTATTAGGTGCTTCTGTTTCTCGTCATTTATCTGTAACAAACCCATTGGCTGCAAGGAGAGATGCCTTACAGAGTACTGTTATTCGCCTCAGAGAGGGTTTCCGCAAAAAAGAACAGAACAGTTCATCAAGCTCGTCTGAAGGTTGTGGCTCAAGCATGAAACGCAGTAGTAGTGTTGAAGCAGGTTGCCTCGGTAATGTTGTTGAAGCAAGTAACAGGAGCGCAACACAGAGCACGGTTGATGCTAATAACTGGAGTAATGTTCTATTACGAACTGCGAGTTCTCACGAGGGGATTAATAGTGATAAAAGCATAGATAGTGGGAGGCCAAGTATAGCATTGTGTAGCAGTTCCTGTCGTTCAGTAATCCAAGAACCTGAAGTGGGAACTTCCTTTACTGACAAAAATTGCGATCAAAATAGCGCATTGGTGGTTTGTTCTAGCAGTGGTCTTGAAAGCCAAGGCTGTGAATCCAGTGCATCAAATTCTGCAAGTCAACAAACTTTAGATTTGAATTTAGCTTTTGCATTGCAAGAAAGGTTGAATGACCCCAGGATAACATCTATGCTAAAGAAGAGGGGAAGACAAGGTGACCTTGAACTAGTTAATCTTTTGCAGGATAAAGGACTGGATCCTAACTTTGTTATGATGTTGAAGGAGAAGAGCTTGGATCCAACCATTCTGGCATTACTTCAAAGAAGTAGTTTGGATGCTGATAGGGATCATCGTGACAATACAGATATCACCATAGCTGACTCAAACAGTGTGGATAATGGCTTACCCAATCAAATCTCTTTATCAGAAGAACTGAGGCTTCACGGGCTTGAAAAATGGCTCCAATTATCTAGGATTGTTTTGCACCATGTAGTAGGTACCCCAGAGAGAGCATGGGTTCTCTTTAGTTTTGTTTTCATCCTCGAAACAATTGCTGTGGCAATTGTCCGTCCAAAGATAATCAAAATAATAAATGCGACACATCAACAGTTTGAATTTGGGTTTGCGGTGCTGCTGTTATCTCCTGTGGTCTGTTCAATCATGGCTTTTCTTCGATCTCTTCAAGCAGAAGAAATGGCCATGACCTCTAAGCCACGCAAGTATGGTTTTGTTGCTTGGCTGCTGAGCACCTGCGTTGGATTATTGCTTTCCTTCTTGAGCAAATCGTCAGTTCTTCTGGGATTGTCTTTGACTGTTCCCCTCATGGTGGCATGCCTTTCTGTTGCCATTCCTACATGGATCCGTAATGGCTACCAATTTTGGGTTCCTCAATTACATTGTGCAGGTCCTGCAGGGAACCATCAAATTCGGGGAACAAAAGAGGGTGTTATTCTTGTCTTCTGCACAACATTATTTACTGGATCTGTATTAGCTCTCGGTGCAATAGTGTCTGCAAAGCCCTTGGATGACTTAAATTACAAAGGATGGACGGGTGAACAGAAAAGCTTTACATCTCCTTATGCATCATCTGTGTACATTGGCTGGGCTATGGCTTCTGCCATTGCTTTACTGGTTACTGGTGTACTGCCAATTGTTTCATGGTTTGCATCTTACCGGTTCTCCATGTTTTCTGCTGTGTGTGTCGGGATATTTACAGCTGTCCTCGTGTCATTTTGTGGTGCATCCTATATTGAGGTTGTGAAATCTAGAGATGACCAAGTTCCAACAAAGGGTGATTTCCTTGCTGCTTTACTTCCTTTAATTTGCATTCCAGCATTTCTGTCTCTGTGCTCGGGGTTGTATAAATGGAAGGATGATAATTGGAAGCTTTCTCGGGGTGTTTATATCTTTGTAACCATCGGTCTTCTACTTCTGCTCGGAGCAATAGCAGCTGTTATAGTCGTAGTTACACCATGGACGATTGGGGTATCATTTCTTTTAGTTCTTCTCATGATTGTACTGGCTATTGGTGCTATACATCATTGGGCCTCAAACAACTTTTATTTGACAAGGACCCAGACATTCTTTGTTTGTTTCCTCGCTTTTCTTTTGGCACTGGCTGCACTTCTTGTTGGATGGTTTGAAGATAAACCATTTGTCGGTGCTTCTGTCGGCTACTTTTTGTTTTTGTTTCTTCTTGCCGGGAGGGCATTGACTGTTCTTCTTTCACCTCCTATAGTTGTTTATTCTCCAAGAGTTCTCCCTGTTTATGTTTATGATGCTCATGCAGACTGTGCAAAGAATGTCAGTGCTGCATTTCTTGTGCTTTATGGGATTGCATTGGCAACTGAGGGCTGGGGTGTTGTTGCGAGTTTGAAAATTTATCCACCATTTGCTGGTGCCGCTGTATCAGCAATTACTCTTGTTGCTGCCTTTGGGTTTGCTTTCTCTCGTCCCTGCTTGACTCTCAAGATGATGGAAGATGCAGTACACTTTCTTAGTAAAGAAACCGTTGTTCAAGCTATTGCTAGATCTGCCACCAAGACCAGAAATGCTTTATCTGGAACTTATTCGGCTCCACAAAGGTCTGCCAGTTCAGCTGCTCTTTTGGTTGGAGATCCCACGATAATGCGTGATAGGGCAGGAAATTTTGTGCTTCCCAGAGCAGATGTCATGAAATTGAGAGATCGCTTAAGAAATGAGGAGTTAGTTGCAGGATCATTCTTCGGAAGAATGAGATATGGAAGGACTTTCCGCCATGAGCCAACAAGTAGTATTGATCACCGAAGAGAGATGTGTGCTCATGCACGAATATTGGCTTTGGAAGAGGCGATTGATACTGAATGGGTATATATGTGGGATAAATTTGGTGGTTATCTGCTTCTTTTGCTTGGTTTGACTGCTAAGGCAGAACGAGTACAGGATGAGGTACGCTTGAGACTCTTTCTTGATAGCATAGGATTTGCAGACTTAAGTGCCAAGAAAATAAAAAAATGGATGCCAGAGGATCGTAGGCAGTTTGAGATTATTCAGGAGAGTTACATAAGAGAAAAGGAGATGGAAGAGGAACTCTTAATGCAAAGACGTGAAGAGGAGGGGAAAGGCAAAGAGAGAAGAAAGGCTCTCTTGGAGAAGGAAGAACGCAAATGGAAGGAGATAGAAGCGTCTTTAATTTCCTCCATTCCTAATGCTGGCAGCAGGGAAGCTGCAGCTGTGGCAGCTGCAGTGCGTGCAGTAGGGGGTGATTCTGTTCTTGATGATTCTTTTGCTCGAGAGAGAGTCTCAAGCATTGCGCGTCGCATACGCACAGCTCAGTTAACTCGGCGAGCACTGCAGACAGGAATTTCCGGTGCTGTATGCGTTCTAGATGATGAACCGACAACAAGTGGCAGGCACTGTGGCCAGATTGATTCTAGCATCTGTCAAAGTCAGAAGATCAGCTTTTCCATTGCAGTGATGATCCAACCTGTGTCTGGGCCTGTTTGCCTGTTAGGCACTGAGTTTCAAAAGAAAGTTTGTTGGGAAATATTGGTCGCCGGTTCTGAGCAAGGAATTGAAGCTGGACAAGTTGGGCTTAGATTGATCACTAAAGGCGACAGACAAACTACTGTTGCAAAGGAGTGGAGTATTAGTGCAACTAGTATTGCAGATGGAAGGTGGCATTTGGTAACAATGACAATTGATGCTGATTTAGGTGAGGCAACTTGCTATCTTGATGGTGGTTTTGATGGCTACCAGACTGGTTTACCATTGCATGTGGGTAATACTATTTGGGAGCAAGGAACAGAAGTTTGGGTAGGTGTTAGACCGCCTACAGATATGGATGCATTTGGGAGGTCAGACAGTGAAGGGGCTGAGTCCAAGATGCATATTATGGATGTGTTTCTATGGGGAAGGTGCTTAACTGAGGATGATATTGCTGCTCTCCATACAGCTGTTGGGTCAGCTGATACTAGTATGATAGATTTTCCTGAAGATGCTTGGCAGTGGGCAGATTCACCTTCTAGACTTGACGAATGGGATAGTGATCCTGCCGATGTAGATCTCTATGATAGGGATGAAGTTGATTGGGATGGGCAATATTCTAGTGGAAGGAAAAGAAGATCGGAGCGTGATGGAGTTCTGGTTGATTTGGATTCCTTTGCCAGGAGGTTTAGGAAACCTAGGATGGAAACGCAAGAAGAAATTAATCAACGAATGCTTTCTGTTGAATTGGCTGTCAAAGAAGCTCTCTGTGCAAGAGGAGAAACAAATTTTACTGATCAGGAGTTTCCTCCAAATGATCAATCACTATTTGTAGATTCGGAAAATCCCCCTTCAAAGTTGCAGGTCGTTTCAGAATGGATGAGGCCTGCTGATATTGTAAAAGAGAGCAGGCTTGCTACTCGGCCGTGCTTATTTTCTGGCACTGTGAATCCTTCTGATGTTTGTCAGGGTCGGCTGGGTGATTGTTGGTTTTTGAGTGCTGTTGCTGTTCTGACGGAAGTTTCTCGAATATCAGAAGTTATTATTACTCCAGAGTATAATGAGGAAGGAATCTACACTGTCCGCTTCTGTATTCAGGGTGAGTGGGTTCCTGTTGTTGTTGATGATTGGATTCCATGTGAATCACCGGGTAAACCAGCATTTGCTACTAGTAGAAAAGGTAACGAGTTGTGGGTCTCTTTACTGGAGAAGGCATATGCCAAATTGCATGGCTCCTATGAAGCATTAGAAGGTGGACTTGTCCAAGATGCTCTTGTAGACCTCACTGGAGGTGCAGGTGAAGAAATTGATATGAGGAGTGCTCAGGCCCAAATTGATCTTGCGAGTGGTCGATTATGGTCTCAATTGTTGCGCTTCAAACAAGAGGGATTTCTTCTGGGTGCTGGAAGTCCATCTGGTTCAGATGTACATATTTCCTCCAGTGGCATTGTGCAAGGTCATGCTTACTCGTTATTGCAGGTGAGAGAGGTGGATGGTCACAAGCTTATTCAGATTCGCAATCCATGGGCCAATGAAGTTGAGTGGAATGGCCCCTGGTCTGACTCATCGCCTGAATGGACTGATAGGATGAAGCATAAGCTGAAGCATATTCCACAGTCAAAAGATGGCATATTCTGGATGTCCTGGCAAGACTTCCAAATTCATTTTCGATCAATATATGTTTGCCGTATCTATCCCCCAGAGATGCGCTACTCTGTTCATGGCCAATGGCGAAGTTATAGTGCTGGTGGCTGCCAAGACTATGAGACATGGCATCAAAATCCACAATTCCGATTGAGGGCCACAGGGCCAGATGCCTCATTTCCAATTCATGTATTCATTACGTTGACACAGGGTGTGAGCTTCTCAAGAACAGTTGCTGGTTTCAGAAACTATCAATCCAGTCATGATTCAATGATGTTCTATATTGGGATGAGGATACTCAAAACTCGAGGCCGCCGAGCTGCTTACAATATATACCTACATGAATCAGTCGGCGGGACAGATTATGTTAATTCACGAGAAATATCTTGTGAAATGGTTTTGGATCCAGATCCAAAAGGTTACACAATAGTGCCGACGACTATACACCCTGGCGAAGAAGCTCCATTTGTTCTTTCTGTTTTCACAAAAGCATCAATAACCTTGGAAGCCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001558 GO:0001708 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0004198 GO:0005198 GO:0005488 GO:0005509 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0005829 GO:0005886 GO:0005887 GO:0006275 GO:0006355 GO:0006508 GO:0006807 GO:0006873 GO:0006874 GO:0006875 GO:0006950 GO:0006970 GO:0006996 GO:0007010 GO:0007204 GO:0007275 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0008307 GO:0009628 GO:0009651 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009934 GO:0009966 GO:0009987 GO:0010008 GO:0010035 GO:0010038 GO:0010154 GO:0010468 GO:0010522 GO:0010524 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010646 GO:0010927 GO:0010941 GO:0010959 GO:0012505 GO:0014717 GO:0014718 GO:0014823 GO:0014850 GO:0016020 GO:0016021 GO:0016043 GO:0016787 GO:0019219 GO:0019222 GO:0019538 GO:0019725 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0030003 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030036 GO:0030154 GO:0030162 GO:0030234 GO:0030239 GO:0030315 GO:0031032 GO:0031090 GO:0031224 GO:0031226 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031402 GO:0031410 GO:0031420 GO:0031432 GO:0031674 GO:0031982 GO:0031984 GO:0032101 GO:0032268 GO:0032269 GO:0032270 GO:0032501 GO:0032502 GO:0032875 GO:0032877 GO:0032879 GO:0032947 GO:0032989 GO:0032991 GO:0033036 GO:0033233 GO:0033234 GO:0033554 GO:0034613 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0040008 GO:0042127 GO:0042175 GO:0042221 GO:0042383 GO:0042592 GO:0042692 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043269 GO:0043270 GO:0043292 GO:0043415 GO:0043416 GO:0043933 GO:0044085 GO:0044093 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044449 GO:0044459 GO:0044464 GO:0045165 GO:0045214 GO:0045740 GO:0045787 GO:0045862 GO:0045892 GO:0045893 GO:0045927 GO:0045934 GO:0045935 GO:0046872 GO:0048316 GO:0048468 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048583 GO:0048584 GO:0048608 GO:0048638 GO:0048639 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050801 GO:0050865 GO:0050867 GO:0050896 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051146 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051239 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051279 GO:0051281 GO:0051282 GO:0051480 GO:0051592 GO:0051641 GO:0051716 GO:0051726 GO:0051924 GO:0051928 GO:0055001 GO:0055002 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055103 GO:0060255 GO:0060341 GO:0060548 GO:0061041 GO:0061061 GO:0061458 GO:0065003 GO:0065007 GO:0065008 GO:0065009 GO:0070011 GO:0070727 GO:0070887 GO:0070925 GO:0071214 GO:0071241 GO:0071248 GO:0071277 GO:0071470 GO:0071472 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072657 GO:0080050 GO:0080090 GO:0080134 GO:0090068 GO:0090303 GO:0090329 GO:0090392 GO:0090627 GO:0090628 GO:0097264 GO:0097435 GO:0097708 GO:0098588 GO:0098771 GO:0098772 GO:0098805 GO:0098827 GO:0099080 GO:0099081 GO:0099512 GO:0104004 GO:0140096 GO:1901564 GO:1902531 GO:1902679 GO:1902680 GO:1903034 GO:1903036 GO:1903169 GO:1903320 GO:1903321 GO:1903506 GO:1903507 GO:1903508 GO:1904062 GO:1904064 GO:1904427 GO:2000011 GO:2000014 GO:2000024 GO:2000026 GO:2000105 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calpain_III Peptidase_C2

Protein Analysis

2160

Amino Acids

239.0

Weight (kDa)

6.05

Isoelectric Point (pI)

39.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C2 PF00648 1705 - 2004 4.6e-89 Calpain family cysteine protease
Calpain_III PF01067 2015 - 2150 1.1e-15 Calpain large subunit, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 2117, 5609, 5791
Acc36I ACCTGC 6 cut(s) 1157, 2117, 2252, 5176, 5609, 5791
Acc65I GGTACC 1 cut(s) 1856
AccB1I GGYRCC 2 cut(s) 1856, 3257
AccB7I CCANNNNNTGG 2 cut(s) 144, 3254
AccI GTMKAC 1 cut(s) 5601
AccII CGCG 1 cut(s) 4153
AciI CCGC 9 cut(s) 472, 1087, 1985, 3261, 3613, 4667, 5407, 6280, 6321
AcoI YGGCCR 5 cut(s) 146, 2055, 4258, 5246, 6046
AcuI CTGAAG 4 cut(s) 1143, 1383, 4851, 5945
AcvI CACGTG 1 cut(s) 22
AdeI CACNNNGTG 1 cut(s) 6179
AflII CTTAAG 2 cut(s) 3550, 3829
AgeI ACCGGT 1 cut(s) 2532
AhlI ACTAGT 4 cut(s) 1585, 4496, 4811, 5492
AjiI CACGTC 1 cut(s) 3944
AjnI CCWGG 6 cut(s) 137, 1535, 5003, 5876, 5966, 6415
AjuI GAANNNNNNNTTGG 6 cut(s) 2226, 2258, 4633, 4665, 5973, 6005
AleI CACNNNNGTG 1 cut(s) 6395
Alw21I GWGCWC 7 cut(s) 374, 1220, 2108, 2711, 3661, 4189, 5647
Alw26I GTCTC 6 cut(s) 2706, 3790, 4144, 5524, 5688, 6083
AlwNI CAGNNNCTG 5 cut(s) 812, 1338, 2267, 4254, 6203
Ama87I CYCGRG 4 cut(s) 2710, 2751, 4128, 6273
AseI ATTAAT 2 cut(s) 1281, 5046
AsiGI ACCGGT 1 cut(s) 2532
Asp700I GAANNNNTTC 2 cut(s) 3427, 5059
Asp718I GGTACC 1 cut(s) 1856
AspA2I CCTAGG 1 cut(s) 5021
AspLEI GCGC 5 cut(s) 1208, 1409, 4153, 5697, 6033
AsuC2I CCSGG 2 cut(s) 3061, 5472
AsuII TTCGAA 1 cut(s) 858
AvaI CYCGRG 4 cut(s) 2710, 2751, 4128, 6273
AvaII GGWCC 4 cut(s) 164, 632, 2264, 2931
AvrII CCTAGG 1 cut(s) 5021
BaeGI GKGCMC 1 cut(s) 954
BalI TGGCCA 4 cut(s) 148, 2057, 4260, 6048
BamHI GGATCC 4 cut(s) 1615, 1654, 2217, 6370
BanI GGYRCC 2 cut(s) 1856, 3257
BanII GRGCYC 1 cut(s) 374
BarI GAAGNNNNNNTAC 2 cut(s) 3330, 3362
BauI CACGAG 4 cut(s) 743, 1271, 2582, 6342
BbrPI CACGTG 1 cut(s) 22
BbsI GAAGAC 3 cut(s) 1573, 2308, 2774
Bbv12I GWGCWC 7 cut(s) 374, 1220, 2108, 2711, 3661, 4189, 5647
BceAI ACGGC 3 cut(s) 94, 5233, 5994
BcgI CGANNNNNNTGC 2 cut(s) 1892, 1926
BciT130I CCWGG 6 cut(s) 139, 1537, 5005, 5878, 5968, 6417
BciVI GTATCC 1 cut(s) 6255
BclI TGATCA 4 cut(s) 3637, 4440, 5119, 5140
BcnI CCSGG 2 cut(s) 3061, 5472
BcoDI GTCTC 6 cut(s) 2706, 3790, 4144, 5524, 5688, 6083
BcuI ACTAGT 4 cut(s) 1585, 4496, 4811, 5492
BfmI CTRYAG 8 cut(s) 121, 541, 2268, 3093, 4065, 4080, 4190, 4671
BfrI CTTAAG 2 cut(s) 3550, 3829
BfuAI ACCTGC 6 cut(s) 1157, 2117, 2252, 5176, 5609, 5791
BfuI GTATCC 1 cut(s) 6255
BglI GCCNNNNNGGC 2 cut(s) 3065, 4350
BglII AGATCT 2 cut(s) 3389, 4900
BlnI CCTAGG 1 cut(s) 5021
BlpI GCTNAGC 1 cut(s) 2102
BmcAI AGTACT 1 cut(s) 1060
Bme1390I CCNGG 8 cut(s) 139, 1537, 3061, 5005, 5472, 5878, 5968, 6417
Bme18I GGWCC 4 cut(s) 164, 632, 2264, 2931
BmeT110I CYCGRG 4 cut(s) 2710, 2751, 4128, 6273
BmgBI CACGTC 1 cut(s) 3944
BmrFI CCNGG 8 cut(s) 139, 1537, 3061, 5005, 5472, 5878, 5968, 6417
BpiI GAAGAC 3 cut(s) 1573, 2308, 2774
BplI GAGNNNNNCTC 4 cut(s) 4124, 4156, 5629, 5661
BpmI CTGGAG 6 cut(s) 210, 1258, 5358, 5549, 5631, 5746
Bpu10I CCTNAGC 2 cut(s) 3762, 5646
Bpu1102I GCTNAGC 1 cut(s) 2102
Bpu14I TTCGAA 1 cut(s) 858
BpuEI CTTGAG 7 cut(s) 358, 1120, 2155, 3311, 3814, 4126, 6175
BpuMI CCSGG 2 cut(s) 3061, 5472
BsaAI YACGTR 1 cut(s) 22
BsaBI GATNNNNATC 4 cut(s) 1704, 4884, 4899, 6375
BsaI GGTCTC 2 cut(s) 5524, 5688
BsaWI WCCGGW 2 cut(s) 2532, 4205
BsaXI ACNNNNNCTCC 8 cut(s) 1300, 1330, 2522, 2552, 4474, 4504, 4968, 4998
Bse118I RCCGGY 3 cut(s) 896, 2532, 4394
Bse3DI GCAATG 6 cut(s) 368, 1511, 2478, 4146, 4305, 4607
Bse8I GATNNNNATC 4 cut(s) 1704, 4884, 4899, 6375
BseBI CCWGG 6 cut(s) 139, 1537, 5005, 5878, 5968, 6417
BseJI GATNNNNATC 4 cut(s) 1704, 4884, 4899, 6375
BseMI GCAATG 6 cut(s) 368, 1511, 2478, 4146, 4305, 4607
BseRI GAGGAG 4 cut(s) 3575, 3965, 5115, 5653
BseSI GKGCMC 1 cut(s) 954
BseX3I CGGCCG 1 cut(s) 5246
BseYI CCCAGC 4 cut(s) 168, 2460, 3213, 5299
BsgI GTGCAG 5 cut(s) 2280, 2305, 2964, 4110, 5637
Bsh1236I CGCG 1 cut(s) 4153
Bsh1285I CGRYCG 1 cut(s) 5249
BshNI GGYRCC 2 cut(s) 1856, 3257
BshTI ACCGGT 1 cut(s) 2532
BsiEI CGRYCG 1 cut(s) 5249
BsiHKAI GWGCWC 7 cut(s) 374, 1220, 2108, 2711, 3661, 4189, 5647
BsiHKCI CYCGRG 4 cut(s) 2710, 2751, 4128, 6273
BsiSI CCGG 6 cut(s) 897, 2533, 3060, 4206, 4395, 5471
BslFI GGGAC 2 cut(s) 3297, 6337
BsmAI GTCTC 6 cut(s) 2706, 3790, 4144, 5524, 5688, 6083
BsmFI GGGAC 2 cut(s) 3297, 6337
BsmI GAATGC 2 cut(s) 2685, 5061
Bso31I GGTCTC 2 cut(s) 5524, 5688
BsoBI CYCGRG 4 cut(s) 2710, 2751, 4128, 6273
Bsp119I TTCGAA 1 cut(s) 858
Bsp1286I GDGCHC 8 cut(s) 374, 954, 1220, 2108, 2711, 3661, 4189, 5647
Bsp1407I TGTACA 2 cut(s) 2451, 5749
Bsp1720I GCTNAGC 1 cut(s) 2102
Bsp19I CCATGG 2 cut(s) 2831, 5846
BspACI CCGC 9 cut(s) 472, 1087, 1985, 3261, 3613, 4667, 5407, 6280, 6321
BspFNI CGCG 1 cut(s) 4153
BspHI TCATGA 3 cut(s) 2868, 3531, 6229
BspMAI CTGCAG 4 cut(s) 2272, 4069, 4084, 4194
BspMI ACCTGC 6 cut(s) 1157, 2117, 2252, 5176, 5609, 5791
BspQI GCTCTTC 2 cut(s) 324, 1640
BspT104I TTCGAA 1 cut(s) 858
BspT107I GGYRCC 2 cut(s) 1856, 3257
BspTI CTTAAG 2 cut(s) 3550, 3829
BspTNI GGTCTC 2 cut(s) 5524, 5688
BsrDI GCAATG 6 cut(s) 368, 1511, 2478, 4146, 4305, 4607
BsrFI RCCGGY 3 cut(s) 896, 2532, 4394
BsrGI TGTACA 2 cut(s) 2451, 5749
BssAI RCCGGY 3 cut(s) 896, 2532, 4394
BssSI CACGAG 4 cut(s) 743, 1271, 2582, 6342
BssT1I CCWWGG 8 cut(s) 516, 721, 1444, 2379, 2831, 5021, 5846, 6468
Bst2BI CACGAG 4 cut(s) 743, 1271, 2582, 6342
Bst2UI CCWGG 6 cut(s) 139, 1537, 5005, 5878, 5968, 6417
Bst6I CTCTTC 8 cut(s) 324, 1553, 1640, 2043, 3640, 3678, 3915, 3942
BstAFI CTTAAG 2 cut(s) 3550, 3829
BstAPI GCANNNNNTGC 4 cut(s) 2267, 2978, 3152, 4088
BstAUI TGTACA 2 cut(s) 2451, 5749
BstBAI YACGTR 1 cut(s) 22
BstBI TTCGAA 1 cut(s) 858
BstDSI CCRYGG 3 cut(s) 110, 2831, 5846
BstEII GGTNACC 1 cut(s) 1574
BstENI CCTNNNNNAGG 2 cut(s) 1076, 5002
BstFNI CGCG 1 cut(s) 4153
BstHHI GCGC 5 cut(s) 1208, 1409, 4153, 5697, 6033
BstMAI GTCTC 6 cut(s) 2706, 3790, 4144, 5524, 5688, 6083
BstMCI CGRYCG 1 cut(s) 5249
BstNI CCWGG 6 cut(s) 139, 1537, 5005, 5878, 5968, 6417
BstNSI RCATGY 5 cut(s) 36, 532, 846, 2193, 4616
BstPI GGTNACC 1 cut(s) 1574
BstSCI CCNGG 8 cut(s) 137, 1535, 3059, 5003, 5470, 5876, 5966, 6415
BstSFI CTRYAG 8 cut(s) 121, 541, 2268, 3093, 4065, 4080, 4190, 4671
BstSLI GKGCMC 1 cut(s) 954
BstUI CGCG 1 cut(s) 4153
BstV2I GAAGAC 3 cut(s) 1573, 2308, 2774
BstZI CGGCCG 1 cut(s) 5246
BsuI GTATCC 1 cut(s) 6255
BtgI CCRYGG 3 cut(s) 110, 2831, 5846
BtgZI GCGATG 1 cut(s) 5874
BtrI CACGTC 1 cut(s) 3944
BtsI GCAGTG 6 cut(s) 952, 1435, 4089, 4187, 4317, 4850
BveI ACCTGC 6 cut(s) 1157, 2117, 2252, 5176, 5609, 5791
CaiI CAGNNNCTG 5 cut(s) 812, 1338, 2267, 4254, 6203
CciI TCATGA 3 cut(s) 2868, 3531, 6229
CfoI GCGC 5 cut(s) 1208, 1409, 4153, 5697, 6033
Cfr10I RCCGGY 3 cut(s) 896, 2532, 4394
CseI GACGC 5 cut(s) 5, 25, 788, 4011, 4142
CspAI ACCGGT 1 cut(s) 2532
CspCI CAANNNNNGTGG 4 cut(s) 1731, 1766, 1896, 1931
DraIII CACNNNGTG 1 cut(s) 6179
EaeI YGGCCR 5 cut(s) 146, 2055, 4258, 5246, 6046
EagI CGGCCG 1 cut(s) 5246
Eam1104I CTCTTC 8 cut(s) 324, 1553, 1640, 2043, 3640, 3678, 3915, 3942
EarI CTCTTC 8 cut(s) 324, 1553, 1640, 2043, 3640, 3678, 3915, 3942
EciI GGCGGA 1 cut(s) 3602
Ecl136II GAGCTC 1 cut(s) 372
EclXI CGGCCG 1 cut(s) 5246
Eco130I CCWWGG 8 cut(s) 516, 721, 1444, 2379, 2831, 5021, 5846, 6468
Eco147I AGGCCT 1 cut(s) 5209
Eco24I GRGCYC 1 cut(s) 374
Eco31I GGTCTC 2 cut(s) 5524, 5688
Eco32I GATATC 2 cut(s) 154, 1726
Eco47I GGWCC 4 cut(s) 164, 632, 2264, 2931
Eco52I CGGCCG 1 cut(s) 5246
Eco53kI GAGCTC 1 cut(s) 372
Eco57I CTGAAG 4 cut(s) 1143, 1383, 4851, 5945
Eco72I CACGTG 1 cut(s) 22
Eco88I CYCGRG 4 cut(s) 2710, 2751, 4128, 6273
Eco91I GGTNACC 1 cut(s) 1574
EcoICRI GAGCTC 1 cut(s) 372
EcoNI CCTNNNNNAGG 2 cut(s) 1076, 5002
EcoO109I RGGNCCY 3 cut(s) 632, 2264, 2931
EcoO65I GGTNACC 1 cut(s) 1574
EcoRII CCWGG 6 cut(s) 137, 1535, 5003, 5876, 5966, 6415
EcoRV GATATC 2 cut(s) 154, 1726
EcoT14I CCWWGG 8 cut(s) 516, 721, 1444, 2379, 2831, 5021, 5846, 6468
EcoT22I ATGCAT 3 cut(s) 2443, 4687, 4728
EcoT38I GRGCYC 1 cut(s) 374
ErhI CCWWGG 8 cut(s) 516, 721, 1444, 2379, 2831, 5021, 5846, 6468
FaqI GGGAC 2 cut(s) 3297, 6337
FauI CCCGC 1 cut(s) 6314
FauNDI CATATG 2 cut(s) 245, 5539
FbaI TGATCA 4 cut(s) 3637, 4440, 5119, 5140
FblI GTMKAC 1 cut(s) 5601
FriOI GRGCYC 1 cut(s) 374
GlaI GCGC 5 cut(s) 1207, 1408, 4152, 5696, 6032
GsaI CCCAGC 4 cut(s) 172, 2464, 3217, 5303
GsuI CTGGAG 6 cut(s) 210, 1258, 5358, 5549, 5631, 5746
HapII CCGG 6 cut(s) 897, 2533, 3060, 4206, 4395, 5471
HgaI GACGC 5 cut(s) 5, 25, 788, 4011, 4142
HhaI GCGC 5 cut(s) 1208, 1409, 4153, 5697, 6033
Hin6I GCGC 5 cut(s) 1206, 1407, 4151, 5695, 6031
HinP1I GCGC 5 cut(s) 1206, 1407, 4151, 5695, 6031
HincII GTYRAC 3 cut(s) 1481, 4176, 6171
HindII GTYRAC 3 cut(s) 1481, 4176, 6171
HindIII AAGCTT 3 cut(s) 2422, 2744, 5825
HpaI GTTAAC 1 cut(s) 4176
HpaII CCGG 6 cut(s) 897, 2533, 3060, 4206, 4395, 5471
Hpy99I CGWCG 2 cut(s) 4158, 6406
HpyCH4IV ACGT 4 cut(s) 21, 784, 3943, 6167
HpySE526I ACGT 4 cut(s) 21, 784, 3943, 6167
HspAI GCGC 5 cut(s) 1206, 1407, 4151, 5695, 6031
KpnI GGTACC 1 cut(s) 1860
KroI GCCGGC 1 cut(s) 896
KroNI GCCGGC 1 cut(s) 898
Ksp22I TGATCA 4 cut(s) 3637, 4440, 5119, 5140
KspAI GTTAAC 1 cut(s) 4176
LguI GCTCTTC 2 cut(s) 324, 1640
MaeII ACGT 4 cut(s) 21, 784, 3943, 6167
MfeI CAATTG 6 cut(s) 510, 1908, 1920, 2508, 4536, 5687
MhlI GDGCHC 8 cut(s) 374, 954, 1220, 2108, 2711, 3661, 4189, 5647
MlsI TGGCCA 4 cut(s) 148, 2057, 4260, 6048
MluNI TGGCCA 4 cut(s) 148, 2057, 4260, 6048
MlyI GAGTC 6 cut(s) 1733, 3316, 3792, 4146, 4724, 5879
MmeI TCCRAC 7 cut(s) 107, 1682, 2095, 2666, 2971, 3451, 4345
Mox20I TGGCCA 4 cut(s) 148, 2057, 4260, 6048
Mph1103I ATGCAT 3 cut(s) 2443, 4687, 4728
MroNI GCCGGC 1 cut(s) 896
MroXI GAANNNNTTC 2 cut(s) 3427, 5059
MscI TGGCCA 4 cut(s) 148, 2057, 4260, 6048
MslI CAYNNNNRTG 3 cut(s) 3485, 4310, 6395
Msp20I TGGCCA 4 cut(s) 148, 2057, 4260, 6048
MspCI CTTAAG 2 cut(s) 3550, 3829
MspI CCGG 6 cut(s) 897, 2533, 3060, 4206, 4395, 5471
MspR9I CCNGG 8 cut(s) 139, 1537, 3061, 5005, 5472, 5878, 5968, 6417
MunI CAATTG 6 cut(s) 510, 1908, 1920, 2508, 4536, 5687
Mva1269I GAATGC 2 cut(s) 2685, 5061
MvaI CCWGG 6 cut(s) 139, 1537, 5005, 5878, 5968, 6417
MvnI CGCG 1 cut(s) 4153
NaeI GCCGGC 1 cut(s) 898
NciI CCSGG 2 cut(s) 3061, 5472
NcoI CCATGG 2 cut(s) 2831, 5846
NdeI CATATG 2 cut(s) 245, 5539
NgoMIV GCCGGC 1 cut(s) 896
NmeAIII GCCGAG 3 cut(s) 4159, 5224, 6308
NmuCI GTSAC 5 cut(s) 17, 195, 1574, 1712, 5819
NsiI ATGCAT 3 cut(s) 2443, 4687, 4728
NspI RCATGY 5 cut(s) 36, 532, 846, 2193, 4616
NspV TTCGAA 1 cut(s) 858
OliI CACNNNNGTG 1 cut(s) 6395
PaeI GCATGC 2 cut(s) 36, 2193
PaeR7I CTCGAG 2 cut(s) 4128, 6273
PagI TCATGA 3 cut(s) 2868, 3531, 6229
PaqCI CACCTGC 3 cut(s) 2117, 5609, 5791
PceI AGGCCT 1 cut(s) 5209
PciSI GCTCTTC 2 cut(s) 324, 1640
PcsI WCGNNNNNNNCGW 1 cut(s) 6280
PctI GAATGC 2 cut(s) 2685, 5061
PdiI GCCGGC 1 cut(s) 898
PdmI GAANNNNTTC 2 cut(s) 3427, 5059
PflMI CCANNNNNTGG 2 cut(s) 144, 3254
PinAI ACCGGT 1 cut(s) 2532
PleI GAGTC 6 cut(s) 1733, 3316, 3792, 4145, 4723, 5879
PmaCI CACGTG 1 cut(s) 22
PmlI CACGTG 1 cut(s) 22
PpsI GAGTC 6 cut(s) 1733, 3316, 3792, 4145, 4723, 5879
Ppu21I YACGTR 1 cut(s) 22
PpuMI RGGWCCY 3 cut(s) 632, 2264, 2931
PshBI ATTAAT 2 cut(s) 1281, 5046
Psp124BI GAGCTC 1 cut(s) 374
Psp5II RGGWCCY 3 cut(s) 632, 2264, 2931
Psp6I CCWGG 6 cut(s) 137, 1535, 5003, 5876, 5966, 6415
PspCI CACGTG 1 cut(s) 22
PspEI GGTNACC 1 cut(s) 1574
PspFI CCCAGC 4 cut(s) 168, 2460, 3213, 5299
PspGI CCWGG 6 cut(s) 137, 1535, 5003, 5876, 5966, 6415
PspPPI RGGWCCY 3 cut(s) 632, 2264, 2931
PspXI VCTCGAGB 1 cut(s) 6273
PsrI GAACNNNNNNTAC 2 cut(s) 1363, 1395
PstI CTGCAG 4 cut(s) 2272, 4069, 4084, 4194
PstNI CAGNNNCTG 5 cut(s) 812, 1338, 2267, 4254, 6203
RseI CAYNNNNRTG 3 cut(s) 3485, 4310, 6395
SacI GAGCTC 1 cut(s) 374
SapI GCTCTTC 2 cut(s) 324, 1640
SbfI CCTGCAGG 1 cut(s) 2272
ScaI AGTACT 1 cut(s) 1060
SchI GAGTC 6 cut(s) 1733, 3316, 3792, 4146, 4724, 5879
ScrFI CCNGG 8 cut(s) 139, 1537, 3061, 5005, 5472, 5878, 5968, 6417
SdaI CCTGCAGG 1 cut(s) 2272
SduI GDGCHC 8 cut(s) 374, 954, 1220, 2108, 2711, 3661, 4189, 5647
SfcI CTRYAG 8 cut(s) 121, 541, 2268, 3093, 4065, 4080, 4190, 4671
Sfr274I CTCGAG 2 cut(s) 4128, 6273
SfuI TTCGAA 1 cut(s) 858
SinI GGWCC 4 cut(s) 164, 632, 2264, 2931
SlaI CTCGAG 2 cut(s) 4128, 6273
SmiMI CAYNNNNRTG 3 cut(s) 3485, 4310, 6395
SpeI ACTAGT 4 cut(s) 1585, 4496, 4811, 5492
SphI GCATGC 2 cut(s) 36, 2193
Sse8387I CCTGCAGG 1 cut(s) 2272
SseBI AGGCCT 1 cut(s) 5209
SsiI CCGC 9 cut(s) 472, 1087, 1985, 3261, 3613, 4667, 5407, 6280, 6321
SspI AATATT 4 cut(s) 270, 3672, 4386, 4940
SstI GAGCTC 1 cut(s) 374
StuI AGGCCT 1 cut(s) 5209
StyD4I CCNGG 8 cut(s) 137, 1535, 3059, 5003, 5470, 5876, 5966, 6415
StyI CCWWGG 8 cut(s) 516, 721, 1444, 2379, 2831, 5021, 5846, 6468
TaiI ACGT 4 cut(s) 24, 787, 3946, 6170
TaqII GACCGA 1 cut(s) 2768
TatI WGTACW 9 cut(s) 235, 747, 1058, 2451, 2500, 2875, 3346, 3775, 5749
TauI GCSGC 2 cut(s) 3263, 6282
TseFI GTSAC 5 cut(s) 17, 195, 1574, 1712, 5819
Tsp45I GTSAC 5 cut(s) 17, 195, 1574, 1712, 5819
TspGWI ACGGA 5 cut(s) 49, 177, 1916, 2210, 5354
Van91I CCANNNNNTGG 2 cut(s) 144, 3254
Vha464I CTTAAG 2 cut(s) 3550, 3829
VpaK11BI GGWCC 4 cut(s) 164, 632, 2264, 2931
VspI ATTAAT 2 cut(s) 1281, 5046
XagI CCTNNNNNAGG 2 cut(s) 1076, 5002
XbaI TCTAGA 3 cut(s) 2624, 4222, 4862
XceI RCATGY 5 cut(s) 36, 532, 846, 2193, 4616
XcmI CCANNNNNNNNNTGG 5 cut(s) 1869, 2485, 2514, 4328, 4793
XhoI CTCGAG 2 cut(s) 4128, 6273
XmaJI CCTAGG 1 cut(s) 5021
XmiI GTMKAC 1 cut(s) 5601
XmnI GAANNNNTTC 2 cut(s) 3427, 5059
ZrmI AGTACT 1 cut(s) 1060
Zsp2I ATGCAT 3 cut(s) 2443, 4687, 4728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.