Rh5BG564500

Belongs to the peptidase C2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0007783
Physical Location & Seq
Reverse (-)
89434363 .. 89450943
16581 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG564500.1

Sequence Viewer

Length: 6180 bp
ATGGCTGGTACTGCTCTTCTACTGGCTTTCTATTCAATCATGCTTTGGTGGCGAACGCAATGGCAGAGCTCAAGGGCTGTTGCTATTCTTCTTCTTCTTGCTGTTGCCCTTCTCTGTGCCTATGAACTCTGTGCTGTATATGTTACAGCTGGTTCAAAAGCATCTCAGCGGTATTCGCCTTCTGGTTTCTTTTTTGGTGTATCAGCAATTGCCTTGGCAATCAACATGCTCTTTATCTGTAGAATGGTGTTTAATGGAAATGGCTTAGATGTCGATGAGTATGTGAGGAAGGCATACAAATTTGCTTATTCTGATTGTATAGAAGTAGGTCCTGTGGCCTGTTTACCAGAACCACCAGATCCGAATGAGTTATATCCTCGACAATCTAGCAGGGCTTCACATCTTGGGCTTCTTTACCTTGGCTCACTTGTAGTTCTTCTCGTGTACTCAATCCTATATGGACTTACTGCAAAGGACGCACGTTGGCTTGGAGCCATTACATCAGCTGCTGTGATAATTCTTGATTGGAACATGGGAGCATGTTTATACGGATTCGAACTCCTTAATAGTCGTGTAGCAGCATTATTTGTTGCCGGCACATCTCGAATTTTTCTCATTTGCTTCGGCGTTCACTACTGGTATTTGGGGCACTGCATTAGTTATGCAGTTGTAGCATCTGTTTTATTAGGTGCTTCTGTTTCTCGTCATTTATCTGTAACAAACCCATTGGCTGCAAGGAGAGATGCCTTACAGAGTACTGTTATTCGCCTCAGAGAGGGTTTCCGCAAAAAAGAACAGAACAGTTCATCAAGCTCATCTGAAGGTTGTGGCTCAAGCATGAAACGCAGTAGTAGTGTTGAAGCAGGTTGCCTTGGTAATGTTGTTGAAGCAAGTCACAGGAGCGCAACACACAGCACGGTTGATGCTAATAACTGGAGTAATGTTCTATTTCGAACTGCGAGTTCTCACGAGGGGATTAATAGTGATAAAAGCATAGATAGTGGGAGGCCAAGTATAGCATTGTGTAGCAGTTCCTGTCGTTCAGTAATCCAAGAGCCTGAAGTGGGAACTTCCTTTACTGACAAAAATTGCGATCAAAATAGCGCATTGGTGGTTTGTTCTAGCAGTGGTCTTGAAAGCCAAGGCTGTGAATCCAGTGCATCAAATTCTGCAAGTCAACAAACTTTAGATTTGAATTTAGCTTTTGCATTGCAAGAAAGGTTGAATGACCCCAGGATAACATCTATGCTAAAGAAGAGGGGAAGACAAAGTGACCTTGAACTAGTTAATCTTTTGCAGGATAAAGGACTGGATCCTAACTTTGTTATGATGTTGAAGGAGAAGAGCTTGGATCCAACCATTCTGGCATTACTTCAAAGAAGTAGTTTGGATGCTGATAGAGATCATCGTGACAATACAGATATCACCATAGCTGACTCAAACAGTGTGGATAATGGTTTACCCAATCAAATCTCTTTATCAGAAGAACTGAGGCTTCACGGGCTTGAAAAATGGCTCAAATTATCTAGGCTTGTTTTGCACCATGTAGTAGGTACCCCAGAGAGAGCATGGGTTCTCTTTAGTTTTGTTTTCATCCTCGAAACAATTGCTGTGGCAATTGTCCGTCCAAAGATAATCAAAATAATAAATGCGACACATCAACAGTTTGAATTTGGGTTTGCGGTGCTGCTGTTATCTCCTGTGGTCTGTTCAATCATGGCTTTTCTTCGATCTCTTCAAGCAGAAGAAATGGCCATGACCTCTAAGCCACGCAAGTATGGTTTTGTTGCTTGGCTGCTGAGCACCTGCGTTGGATTATTGCTTTCCTTCTTGAGTAAATCATCAGTTCTTCTGGGATTGTCTTTGACTGTTCCCCTCATGGTGGCATGCCTTTCTGTTGCCATTCCTACATGGATCCGTAATGGCTACCAATTTTGGGTTCCTCAATTACATTGTGCAGGTCCTGCAGGGAACCATCAAATTCGGGGAACAAAAGAGGGTGTTATTCTTGTCTTCTGCACAACATTATTTACTGGATCTGTATTAGCTCTCGGTGCAATAGTGTCTGCAAAGCCCTTGGATGACTTAAATTACAAAGGATGGACGGGTGAACAGAAAAGCTTTACATCTCCTTATGCATCATCTGTGTACATTGGCTGGGCTATGGCTTCTGCCATTGCTTTAGTGGTTACTGGTGTACTGCCAATTGTTTCATGGTTTGCATCTTACCGGTTCTCCATGTTTTCTGCTGTGTGTGTCGGGATATTTACAGCTGTCCTCGTGTCATTTTGTGGTGCATCCTATATTGAGGTTGTGAAATCTAGAGATGACCAAGTTCCAACAAAGGGTGATTTCCTTGCTGCTTTACTTCCTTTAATTTGCATTCCAGCATTTCTGTCTCTGTGCTCGGGGTTGTATAAATGGAAGGATGATAATTGGAAGCTTTCTCGGGGTGTTTATATCTTTGTAACCATCGGTCTTCTACTTCTGCTCGGAGCAATATCAGCTGTTATAGTCGTAGTTACACCATGGACGGTTGGGGTATCATTTCTTTTAGTTCTTCTCATGATTGTACTGGCTATTGGTGCTATACATCATTGGGCCTCAAACAACTTTTATTTGACAAGGACCCAGACATTCTTTGTCTGTTTCCTCGCTTTTCTTTTGGCACTGGCTGCACTTCTTGTTGGATGGTTTGAAGATAAACCATTTGTCGGTGCTTCTGTCGGCTACTTTTTGTTTTTGTTTCTTCTTGCCGGGAGGGCATTGACTGTTCTTCTTTCACCTCCTATAGTTGTTTATTCTCCAAGAGTTCTCCCTGTTTATGTTTATGATGCTCATGCAGACTGTGCAAAGAATGTCAGTGCTGCATTTCTTGTGCTTTATGGGATTGCATTGGCAACTGAGGGCTGGGGTGTTGTTGCGAGTTTGAAAATTTATCCACCATTTGCTGGTGCCGCTGTATCAGCAATTACTCTTGTTGCTGCCTTTGGGTTTGCTTTCTCTCGTCCCTGCTTGACTCTCAAGATGATGGAAGATGCGGTACACTTTCTTAGTAAAGAAACCGTTGTTCAAGCTATTGCTAGATCTGCCACCAAGACCAGAAATGCTTTATCTGGAACTTATTCGGCTCCACAAAGGTCTGCCAGTTCAGCTGCTCTTTTGGTTGGAGATCCCACGATTATGCGTGATAGGGCAGGAAATTTTGTGCTTCCCAGAGCAGATGTCATGAAATTGAGAGATCGCTTAAGAAATGAGGAGTTAGTTGCAGGATCATTCTTCGGAAGAATGAGATATGGAAGGACTTTCCGCCATGAGCCAACAAGTAGTATTGATCACCGAAGAGAGATGTGTGCTCATGCACGAATATTGGCTTTGGAAGAGGCGATTGATACAGAATGGGTATATATGTGGGATAAATTTGGTGGTTATCTGCTTCTTTTGCTTGGTTTGACTGCTAAGGCAGAGCGGGTACAGGATGAGGTACGCTTGAGACTCTTTCTTGATAGCATAGGATTTGCAGACTTAAGTGCCAAGAAAATAAAAAAATGGATGCCAGAGGATCGTAGGCAGTTTGAGATTATTCAGGAGAGTTACATAAGAGAAAAGGAGATGGAAGAGGAACTCTTAATGCAAAGACGTGAAGAGGAGGGGAAAGGCAAAGAGAGAAGAAAGGCTCTCTTGGAGAAGGAAGAACGCAAATGGAAGGAGATAGAAGCGTCTTTAATTTCCTCCATTCCTAATGCTGGCAGCAGGGAAGCTGCAGCTGTGGCAGCTGCAGTGCGTGCAGTAGGGGGTGATTCTGTTCTTGATGATTCTTTTGCTCGAGAGAGAGTCTCAAGCATTGCGCGTCGCATACGCACAGCTCAGTTAACTCGGCGAGCACTGCAGACAGGAATTTCCGGAGCTGTATGCGTTCTAGATGATGAACCGACAACAAGTGGCAGGCACTGTGGCCAGATTGATTCTAGCATCTGTCAAAGTCAGAAGATCAGCTTTTCCATTGCAGTGATGATCCAACCTGTGTCTGGGCCTGTTTGCCTATTAGGCACTGAGTTTCAAAAGAAAGTTTGTTGGGAAATATTGGTCGCCGGTTCTGAGCAAGGAATTGAAGCTGGACAAGTTGGGCTTAGATTGATCACTAAAGGCGACAGACAAACTACTGTTGCAAAGGAGTGGAGTATTAGTGCAACTAGTATTGCAGATGGAAGGTGGCATTTGGTAACAATGACAATTGATGCTGATTTAGGTGAGGCAACTTGCTATCTCGATGGTGGTTTTGATGGCTACCAGACTGGTTTACCATTGCATGTGGGTAATACTATTTGGGAGCAAGGAACAGAAGTTTGGGTAGGTGTTAGACCGCCTACAGATATGGATGCATTTGGGAGGTCAGACAGTGAAGGGGCTGAGTCCAAGATGCATATTATGGATGTGTTTCTATGGGGAAGGTGCTTAACTGAGGATGATATTGCTGCTCTCCATACAGCTGTTGGGTCAGCTGATACTAGTATGATAGATTTTCCTGAAGATGCTTGGCAGTGGGCAGATTCACCTTCTAGACTTGATGAATGGGATAGTGATCCTGCCGATGTAGATCTATATGATAGGGATGAAGTTGATTGGGATGGGCAATATTCTAGTGGAAGGAAAAGAAGATCAGAGCGTGATGGAGTTCTGGTTGATTTGGATTCCTTTGCCAGGAGGTTTAGGAAACCTAGGATGGAAACGCAAGAAGAAATTAATCAACGAATGCTTTCTGTTGAATTGGCCGTCAAAGAAGCTCTCTGTGCAAGAGGAGAAACAAATTTTACTGATCAGGAGTTTCCTCCAAATGATCAATCACTATTTGTAGATTCGGAAAATCCCCCTTCAAAGTTGCAGGTCGTTTCAGAATGGATGAGGCCTGCTGATATTGTAAAAGAGAGCAGGCTTGCTACTCGGCCGTGCTTATTTTCTGGCACTGTGAATCCTTCTGATGTTTGTCAGGGTCGGCTGGGTGATTGTTGGTTTTTGAGTGCTGTTGCTGTTCTGACGGAAGTTTCTCGAATATCAGAAGTTATTATTACTCCAGAGTATAATGAGGAAGGAATCTACACTGTCCGCTTCTGTATTCAGGGTGAGTGGGTTCCTGTTGTTGTTGATGATTGGATTCCGTGTGAATCACCGGGTAAACCAGCATTTGCTACTAGTAGAAAAGGTAACGAGTTGTGGGTCTCTTTACTGGAGAAGGCATATGCCAAATTGCATGGCTCCTATGAAGCATTAGAAGGTGGACTTGTCCAAGATGCTCTTGTAGACCTCACTGGAGGTGCAGGTGAAGAAATTGATATGAGGAGTGCTCAGGCCCAAATTGATCTTGCGAGTGGTCGATTATGGTCTCAATTGTTGCGCTTCAAACAAGAGGGATTTCTTCTGGGTGCTGGAAGTCCATCTGGTTCAGATGTACATATTTCCTCCAGTGGCATTGTGCAAGGTCATGCTTACTCGTTATTGCAGGTGAGAGAGGTGGATGGTCACAAGCTTATTCAGATTCGCAATCCATGGGCCAATGAAGTTGAGTGGAATGGCCCCTGGTCTGACTCATCGCCTGAATGGACTGATAGGATGAAGCATAAGCTGAAGCATATTCCACAGTCAAAAGATGGCATATTCTGGATGTCCTGGCAAGACTTCCAAATTCATTTTCGATCAATATATGTTTGCCGTATCTATCCCCCTGAGATGCGCTACTCTGTTCATGGCCAATGGCGAAGTTATAGTGCTGGTGGCTGCCAAGACTATGAGACATGGCATCAAAATCCACAATTCCGATTGAGGGCCACAGGGCCAGATGCCTCATTTCCAATTCATGTATTCATTACGTTGACACAGGGTGTGAGCTTCTCAAGAACAGTTGCTGGTTTCAGAAACTATCAATCCAGTCATGATTCAATGATGTTCTATATTGGGATGAGGATACTCAAAACTCGAGGTCGCCGAGCTGCTTACAATATATACCTACATGAATCCGTCGGTGGGACAGATTATGTTAATTCACGAGAAATATCTTGTGAAATGGTTTTGGATCCAGATCCAAAAGGTTACACAATAGTGCCGACAACTATACACCCTGGCGAAGAAGCTCCATTTGTTCTTTCTGTTTTCACAAAAGCATCAATAACCTTGGAAGCCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001558 GO:0001708 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0004198 GO:0005198 GO:0005488 GO:0005509 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0005829 GO:0005886 GO:0005887 GO:0006275 GO:0006355 GO:0006508 GO:0006807 GO:0006873 GO:0006874 GO:0006875 GO:0006950 GO:0006970 GO:0006996 GO:0007010 GO:0007204 GO:0007275 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0008307 GO:0009628 GO:0009651 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009934 GO:0009966 GO:0009987 GO:0010008 GO:0010035 GO:0010038 GO:0010154 GO:0010468 GO:0010522 GO:0010524 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010646 GO:0010927 GO:0010941 GO:0010959 GO:0012505 GO:0014717 GO:0014718 GO:0014823 GO:0014850 GO:0016020 GO:0016021 GO:0016043 GO:0016787 GO:0019219 GO:0019222 GO:0019538 GO:0019725 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0030003 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030036 GO:0030154 GO:0030162 GO:0030234 GO:0030239 GO:0030315 GO:0031032 GO:0031090 GO:0031224 GO:0031226 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031402 GO:0031410 GO:0031420 GO:0031432 GO:0031674 GO:0031982 GO:0031984 GO:0032101 GO:0032268 GO:0032269 GO:0032270 GO:0032501 GO:0032502 GO:0032875 GO:0032877 GO:0032879 GO:0032947 GO:0032989 GO:0032991 GO:0033036 GO:0033233 GO:0033234 GO:0033554 GO:0034613 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0040008 GO:0042127 GO:0042175 GO:0042221 GO:0042383 GO:0042592 GO:0042692 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043269 GO:0043270 GO:0043292 GO:0043415 GO:0043416 GO:0043933 GO:0044085 GO:0044093 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044449 GO:0044459 GO:0044464 GO:0045165 GO:0045214 GO:0045740 GO:0045787 GO:0045862 GO:0045892 GO:0045893 GO:0045927 GO:0045934 GO:0045935 GO:0046872 GO:0048316 GO:0048468 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048583 GO:0048584 GO:0048608 GO:0048638 GO:0048639 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050801 GO:0050865 GO:0050867 GO:0050896 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051146 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051239 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051279 GO:0051281 GO:0051282 GO:0051480 GO:0051592 GO:0051641 GO:0051716 GO:0051726 GO:0051924 GO:0051928 GO:0055001 GO:0055002 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055103 GO:0060255 GO:0060341 GO:0060548 GO:0061041 GO:0061061 GO:0061458 GO:0065003 GO:0065007 GO:0065008 GO:0065009 GO:0070011 GO:0070727 GO:0070887 GO:0070925 GO:0071214 GO:0071241 GO:0071248 GO:0071277 GO:0071470 GO:0071472 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072657 GO:0080050 GO:0080090 GO:0080134 GO:0090068 GO:0090303 GO:0090329 GO:0090392 GO:0090627 GO:0090628 GO:0097264 GO:0097435 GO:0097708 GO:0098588 GO:0098771 GO:0098772 GO:0098805 GO:0098827 GO:0099080 GO:0099081 GO:0099512 GO:0104004 GO:0140096 GO:1901564 GO:1902531 GO:1902679 GO:1902680 GO:1903034 GO:1903036 GO:1903169 GO:1903320 GO:1903321 GO:1903506 GO:1903507 GO:1903508 GO:1904062 GO:1904064 GO:1904427 GO:2000011 GO:2000014 GO:2000024 GO:2000026 GO:2000105 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calpain_III Peptidase_C2

Protein Analysis

2059

Amino Acids

227.62

Weight (kDa)

6.07

Isoelectric Point (pI)

39.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C2 PF00648 1604 - 1903 4.3e-89 Calpain family cysteine protease
Calpain_III PF01067 1914 - 2049 1e-15 Calpain large subunit, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 1814, 5306, 5488
Acc36I ACCTGC 6 cut(s) 854, 1814, 1949, 4873, 5306, 5488
Acc65I GGTACC 1 cut(s) 1553
AccB1I GGYRCC 2 cut(s) 1553, 2954
AccB7I CCANNNNNTGG 1 cut(s) 2951
AccBSI CCGCTC 1 cut(s) 3469
AccI GTMKAC 1 cut(s) 5298
AccII CGCG 1 cut(s) 3850
AccIII TCCGGA 1 cut(s) 3902
AciI CCGC 9 cut(s) 169, 784, 1682, 2958, 3041, 3310, 3469, 4364, 5104
AcoI YGGCCR 5 cut(s) 1752, 3955, 4770, 4943, 5743
AcuI CTGAAG 4 cut(s) 840, 1080, 4548, 5642
AdeI CACNNNGTG 1 cut(s) 5876
AflII CTTAAG 2 cut(s) 3247, 3526
AgeI ACCGGT 1 cut(s) 2229
AhlI ACTAGT 4 cut(s) 1282, 4193, 4508, 5189
AjiI CACGTC 1 cut(s) 3641
AjnI CCWGG 5 cut(s) 1232, 4700, 5573, 5663, 6112
AjuI GAANNNNNNNTTGG 6 cut(s) 1923, 1955, 4330, 4362, 5670, 5702
AleI CACNNNNGTG 1 cut(s) 6092
Alw21I GWGCWC 6 cut(s) 71, 1805, 2408, 3358, 3886, 5344
Alw26I GTCTC 6 cut(s) 2403, 3487, 3841, 5221, 5385, 5780
AlwNI CAGNNNCTG 5 cut(s) 509, 1035, 1964, 3951, 5900
Ama87I CYCGRG 4 cut(s) 2407, 2448, 3825, 5970
Aor13HI TCCGGA 1 cut(s) 3902
AseI ATTAAT 2 cut(s) 978, 4743
AsiGI ACCGGT 1 cut(s) 2229
Asp700I GAANNNNTTC 2 cut(s) 3124, 4756
Asp718I GGTACC 1 cut(s) 1553
AspA2I CCTAGG 1 cut(s) 4718
AspLEI GCGC 5 cut(s) 905, 1106, 3850, 5394, 5730
AsuC2I CCSGG 2 cut(s) 2758, 5169
AsuII TTCGAA 2 cut(s) 555, 952
AvaI CYCGRG 4 cut(s) 2407, 2448, 3825, 5970
AvaII GGWCC 3 cut(s) 329, 1961, 2628
AvrII CCTAGG 1 cut(s) 4718
BaeGI GKGCMC 1 cut(s) 651
BalI TGGCCA 3 cut(s) 1754, 3957, 5745
BamHI GGATCC 4 cut(s) 1312, 1351, 1914, 6067
BanI GGYRCC 2 cut(s) 1553, 2954
BanII GRGCYC 1 cut(s) 71
BarI GAAGNNNNNNTAC 2 cut(s) 3027, 3059
BauI CACGAG 4 cut(s) 440, 968, 2279, 6039
BbsI GAAGAC 3 cut(s) 1270, 2005, 2471
Bbv12I GWGCWC 6 cut(s) 71, 1805, 2408, 3358, 3886, 5344
BceAI ACGGC 3 cut(s) 4757, 4930, 5691
BcgI CGANNNNNNTGC 2 cut(s) 1589, 1623
BciT130I CCWGG 5 cut(s) 1234, 4702, 5575, 5665, 6114
BciVI GTATCC 1 cut(s) 5952
BclI TGATCA 4 cut(s) 3334, 4137, 4816, 4837
BcnI CCSGG 2 cut(s) 2758, 5169
BcoDI GTCTC 6 cut(s) 2403, 3487, 3841, 5221, 5385, 5780
BcuI ACTAGT 4 cut(s) 1282, 4193, 4508, 5189
BfmI CTRYAG 7 cut(s) 238, 1965, 2790, 3762, 3777, 3887, 4368
BfrI CTTAAG 2 cut(s) 3247, 3526
BfuAI ACCTGC 6 cut(s) 854, 1814, 1949, 4873, 5306, 5488
BfuI GTATCC 1 cut(s) 5952
BglI GCCNNNNNGGC 2 cut(s) 2762, 4047
BglII AGATCT 2 cut(s) 3086, 4597
BlnI CCTAGG 1 cut(s) 4718
BlpI GCTNAGC 1 cut(s) 1799
BmcAI AGTACT 1 cut(s) 757
Bme1390I CCNGG 7 cut(s) 1234, 2758, 4702, 5169, 5575, 5665, 6114
Bme18I GGWCC 3 cut(s) 329, 1961, 2628
BmeT110I CYCGRG 4 cut(s) 2407, 2448, 3825, 5970
BmgBI CACGTC 1 cut(s) 3641
BmrFI CCNGG 7 cut(s) 1234, 2758, 4702, 5169, 5575, 5665, 6114
BpiI GAAGAC 3 cut(s) 1270, 2005, 2471
BplI GAGNNNNNCTC 4 cut(s) 3821, 3853, 5326, 5358
BpmI CTGGAG 5 cut(s) 955, 5055, 5246, 5328, 5443
Bpu10I CCTNAGC 2 cut(s) 3459, 5343
Bpu1102I GCTNAGC 1 cut(s) 1799
Bpu14I TTCGAA 2 cut(s) 555, 952
BpuEI CTTGAG 7 cut(s) 55, 817, 1852, 3008, 3511, 3823, 5872
BpuMI CCSGG 2 cut(s) 2758, 5169
BsaBI GATNNNNATC 4 cut(s) 1401, 4581, 4596, 6072
BsaI GGTCTC 2 cut(s) 5221, 5385
BsaWI WCCGGW 2 cut(s) 2229, 3902
BsaXI ACNNNNNCTCC 8 cut(s) 997, 1027, 2219, 2249, 4171, 4201, 4665, 4695
Bse118I RCCGGY 3 cut(s) 593, 2229, 4091
Bse3DI GCAATG 6 cut(s) 65, 1208, 2175, 3843, 4002, 4304
Bse8I GATNNNNATC 4 cut(s) 1401, 4581, 4596, 6072
BseAI TCCGGA 1 cut(s) 3902
BseBI CCWGG 5 cut(s) 1234, 4702, 5575, 5665, 6114
BseJI GATNNNNATC 4 cut(s) 1401, 4581, 4596, 6072
BseMI GCAATG 6 cut(s) 65, 1208, 2175, 3843, 4002, 4304
BseRI GAGGAG 4 cut(s) 3272, 3662, 4812, 5350
BseSI GKGCMC 1 cut(s) 651
BseX3I CGGCCG 1 cut(s) 4943
BseYI CCCAGC 3 cut(s) 2157, 2910, 4996
BsgI GTGCAG 5 cut(s) 1977, 2002, 2661, 3807, 5334
Bsh1236I CGCG 1 cut(s) 3850
Bsh1285I CGRYCG 1 cut(s) 4946
BshNI GGYRCC 2 cut(s) 1553, 2954
BshTI ACCGGT 1 cut(s) 2229
BsiEI CGRYCG 1 cut(s) 4946
BsiHKAI GWGCWC 6 cut(s) 71, 1805, 2408, 3358, 3886, 5344
BsiHKCI CYCGRG 4 cut(s) 2407, 2448, 3825, 5970
BsiSI CCGG 6 cut(s) 594, 2230, 2757, 3903, 4092, 5168
BslFI GGGAC 2 cut(s) 2994, 6034
BsmAI GTCTC 6 cut(s) 2403, 3487, 3841, 5221, 5385, 5780
BsmFI GGGAC 2 cut(s) 2994, 6034
BsmI GAATGC 2 cut(s) 2382, 4758
Bso31I GGTCTC 2 cut(s) 5221, 5385
BsoBI CYCGRG 4 cut(s) 2407, 2448, 3825, 5970
Bsp119I TTCGAA 2 cut(s) 555, 952
Bsp1286I GDGCHC 7 cut(s) 71, 651, 1805, 2408, 3358, 3886, 5344
Bsp13I TCCGGA 1 cut(s) 3902
Bsp1407I TGTACA 2 cut(s) 2148, 5446
Bsp1720I GCTNAGC 1 cut(s) 1799
Bsp19I CCATGG 2 cut(s) 2528, 5543
BspACI CCGC 9 cut(s) 169, 784, 1682, 2958, 3041, 3310, 3469, 4364, 5104
BspEI TCCGGA 1 cut(s) 3902
BspFNI CGCG 1 cut(s) 3850
BspHI TCATGA 3 cut(s) 2565, 3228, 5926
BspMAI CTGCAG 4 cut(s) 1969, 3766, 3781, 3891
BspMI ACCTGC 6 cut(s) 854, 1814, 1949, 4873, 5306, 5488
BspQI GCTCTTC 2 cut(s) 21, 1337
BspT104I TTCGAA 2 cut(s) 555, 952
BspT107I GGYRCC 2 cut(s) 1553, 2954
BspTI CTTAAG 2 cut(s) 3247, 3526
BspTNI GGTCTC 2 cut(s) 5221, 5385
BsrBI CCGCTC 1 cut(s) 3469
BsrDI GCAATG 6 cut(s) 65, 1208, 2175, 3843, 4002, 4304
BsrFI RCCGGY 3 cut(s) 593, 2229, 4091
BsrGI TGTACA 2 cut(s) 2148, 5446
BssAI RCCGGY 3 cut(s) 593, 2229, 4091
BssSI CACGAG 4 cut(s) 440, 968, 2279, 6039
BssT1I CCWWGG 9 cut(s) 213, 418, 871, 1141, 2076, 2528, 4718, 5543, 6165
Bst2BI CACGAG 4 cut(s) 440, 968, 2279, 6039
Bst2UI CCWGG 5 cut(s) 1234, 4702, 5575, 5665, 6114
Bst6I CTCTTC 8 cut(s) 21, 1250, 1337, 1740, 3337, 3375, 3612, 3639
BstAFI CTTAAG 2 cut(s) 3247, 3526
BstAPI GCANNNNNTGC 4 cut(s) 1964, 2675, 2849, 3785
BstAUI TGTACA 2 cut(s) 2148, 5446
BstBI TTCGAA 2 cut(s) 555, 952
BstC8I GCNNGC 9 cut(s) 595, 1888, 3748, 3786, 3882, 3947, 4908, 4931, 4935
BstDSI CCRYGG 2 cut(s) 2528, 5543
BstENI CCTNNNNNAGG 2 cut(s) 773, 4699
BstFNI CGCG 1 cut(s) 3850
BstHHI GCGC 5 cut(s) 905, 1106, 3850, 5394, 5730
BstMAI GTCTC 6 cut(s) 2403, 3487, 3841, 5221, 5385, 5780
BstMCI CGRYCG 1 cut(s) 4946
BstNI CCWGG 5 cut(s) 1234, 4702, 5575, 5665, 6114
BstNSI RCATGY 4 cut(s) 229, 543, 1890, 4313
BstSCI CCNGG 7 cut(s) 1232, 2756, 4700, 5167, 5573, 5663, 6112
BstSFI CTRYAG 7 cut(s) 238, 1965, 2790, 3762, 3777, 3887, 4368
BstSLI GKGCMC 1 cut(s) 651
BstUI CGCG 1 cut(s) 3850
BstV2I GAAGAC 3 cut(s) 1270, 2005, 2471
BstZI CGGCCG 1 cut(s) 4943
BsuI GTATCC 1 cut(s) 5952
BtgI CCRYGG 2 cut(s) 2528, 5543
BtgZI GCGATG 1 cut(s) 5571
BtrI CACGTC 1 cut(s) 3641
BtsI GCAGTG 6 cut(s) 649, 1132, 3786, 3884, 4014, 4547
BveI ACCTGC 6 cut(s) 854, 1814, 1949, 4873, 5306, 5488
Cac8I GCNNGC 9 cut(s) 595, 1888, 3748, 3786, 3882, 3947, 4908, 4931, 4935
CaiI CAGNNNCTG 5 cut(s) 509, 1035, 1964, 3951, 5900
CciI TCATGA 3 cut(s) 2565, 3228, 5926
CfoI GCGC 5 cut(s) 905, 1106, 3850, 5394, 5730
Cfr10I RCCGGY 3 cut(s) 593, 2229, 4091
CseI GACGC 3 cut(s) 485, 3708, 3839
CspAI ACCGGT 1 cut(s) 2229
CspCI CAANNNNNGTGG 4 cut(s) 1428, 1463, 1593, 1628
DraIII CACNNNGTG 1 cut(s) 5876
EaeI YGGCCR 5 cut(s) 1752, 3955, 4770, 4943, 5743
EagI CGGCCG 1 cut(s) 4943
Eam1104I CTCTTC 8 cut(s) 21, 1250, 1337, 1740, 3337, 3375, 3612, 3639
EarI CTCTTC 8 cut(s) 21, 1250, 1337, 1740, 3337, 3375, 3612, 3639
EciI GGCGGA 1 cut(s) 3299
Ecl136II GAGCTC 1 cut(s) 69
EclXI CGGCCG 1 cut(s) 4943
Eco130I CCWWGG 9 cut(s) 213, 418, 871, 1141, 2076, 2528, 4718, 5543, 6165
Eco147I AGGCCT 1 cut(s) 4906
Eco24I GRGCYC 1 cut(s) 71
Eco31I GGTCTC 2 cut(s) 5221, 5385
Eco32I GATATC 1 cut(s) 1423
Eco47I GGWCC 3 cut(s) 329, 1961, 2628
Eco52I CGGCCG 1 cut(s) 4943
Eco53kI GAGCTC 1 cut(s) 69
Eco57I CTGAAG 4 cut(s) 840, 1080, 4548, 5642
Eco88I CYCGRG 4 cut(s) 2407, 2448, 3825, 5970
EcoICRI GAGCTC 1 cut(s) 69
EcoNI CCTNNNNNAGG 2 cut(s) 773, 4699
EcoO109I RGGNCCY 3 cut(s) 329, 1961, 2628
EcoRII CCWGG 5 cut(s) 1232, 4700, 5573, 5663, 6112
EcoRV GATATC 1 cut(s) 1423
EcoT14I CCWWGG 9 cut(s) 213, 418, 871, 1141, 2076, 2528, 4718, 5543, 6165
EcoT22I ATGCAT 3 cut(s) 2140, 4384, 4425
EcoT38I GRGCYC 1 cut(s) 71
ErhI CCWWGG 9 cut(s) 213, 418, 871, 1141, 2076, 2528, 4718, 5543, 6165
FaqI GGGAC 2 cut(s) 2994, 6034
FauI CCCGC 1 cut(s) 3462
FauNDI CATATG 1 cut(s) 5236
FbaI TGATCA 4 cut(s) 3334, 4137, 4816, 4837
FblI GTMKAC 1 cut(s) 5298
FriOI GRGCYC 1 cut(s) 71
GlaI GCGC 5 cut(s) 904, 1105, 3849, 5393, 5729
GsaI CCCAGC 3 cut(s) 2161, 2914, 5000
GsuI CTGGAG 5 cut(s) 955, 5055, 5246, 5328, 5443
HapII CCGG 6 cut(s) 594, 2230, 2757, 3903, 4092, 5168
HgaI GACGC 3 cut(s) 485, 3708, 3839
HhaI GCGC 5 cut(s) 905, 1106, 3850, 5394, 5730
Hin6I GCGC 5 cut(s) 903, 1104, 3848, 5392, 5728
HinP1I GCGC 5 cut(s) 903, 1104, 3848, 5392, 5728
HincII GTYRAC 3 cut(s) 1178, 3873, 5868
HindII GTYRAC 3 cut(s) 1178, 3873, 5868
HindIII AAGCTT 3 cut(s) 2119, 2441, 5522
HpaI GTTAAC 1 cut(s) 3873
HpaII CCGG 6 cut(s) 594, 2230, 2757, 3903, 4092, 5168
Hpy99I CGWCG 2 cut(s) 3855, 6017
HpyCH4IV ACGT 3 cut(s) 481, 3640, 5864
HpySE526I ACGT 3 cut(s) 481, 3640, 5864
HspAI GCGC 5 cut(s) 903, 1104, 3848, 5392, 5728
Kpn2I TCCGGA 1 cut(s) 3902
KpnI GGTACC 1 cut(s) 1557
KroI GCCGGC 1 cut(s) 593
KroNI GCCGGC 1 cut(s) 595
Ksp22I TGATCA 4 cut(s) 3334, 4137, 4816, 4837
KspAI GTTAAC 1 cut(s) 3873
LguI GCTCTTC 2 cut(s) 21, 1337
LmnI GCTCC 9 cut(s) 491, 536, 900, 2495, 3136, 3905, 4330, 5258, 6130
MaeII ACGT 3 cut(s) 481, 3640, 5864
MbiI CCGCTC 1 cut(s) 3469
MfeI CAATTG 6 cut(s) 207, 1605, 1617, 2205, 4233, 5384
MhlI GDGCHC 7 cut(s) 71, 651, 1805, 2408, 3358, 3886, 5344
MlsI TGGCCA 3 cut(s) 1754, 3957, 5745
MluNI TGGCCA 3 cut(s) 1754, 3957, 5745
MlyI GAGTC 6 cut(s) 1430, 3013, 3489, 3843, 4421, 5576
MmeI TCCRAC 6 cut(s) 1379, 1792, 2363, 2668, 3148, 4042
Mox20I TGGCCA 3 cut(s) 1754, 3957, 5745
Mph1103I ATGCAT 3 cut(s) 2140, 4384, 4425
MroI TCCGGA 1 cut(s) 3902
MroNI GCCGGC 1 cut(s) 593
MroXI GAANNNNTTC 2 cut(s) 3124, 4756
MscI TGGCCA 3 cut(s) 1754, 3957, 5745
MslI CAYNNNNRTG 3 cut(s) 3182, 4007, 6092
Msp20I TGGCCA 3 cut(s) 1754, 3957, 5745
MspCI CTTAAG 2 cut(s) 3247, 3526
MspI CCGG 6 cut(s) 594, 2230, 2757, 3903, 4092, 5168
MspR9I CCNGG 7 cut(s) 1234, 2758, 4702, 5169, 5575, 5665, 6114
MunI CAATTG 6 cut(s) 207, 1605, 1617, 2205, 4233, 5384
Mva1269I GAATGC 2 cut(s) 2382, 4758
MvaI CCWGG 5 cut(s) 1234, 4702, 5575, 5665, 6114
MvnI CGCG 1 cut(s) 3850
NaeI GCCGGC 1 cut(s) 595
NciI CCSGG 2 cut(s) 2758, 5169
NcoI CCATGG 2 cut(s) 2528, 5543
NdeI CATATG 1 cut(s) 5236
NgoMIV GCCGGC 1 cut(s) 593
NmeAIII GCCGAG 3 cut(s) 3856, 4921, 6005
NmuCI GTSAC 4 cut(s) 893, 1271, 1409, 5516
NsiI ATGCAT 3 cut(s) 2140, 4384, 4425
NspI RCATGY 4 cut(s) 229, 543, 1890, 4313
NspV TTCGAA 2 cut(s) 555, 952
OliI CACNNNNGTG 1 cut(s) 6092
PaeI GCATGC 1 cut(s) 1890
PaeR7I CTCGAG 2 cut(s) 3825, 5970
PagI TCATGA 3 cut(s) 2565, 3228, 5926
PaqCI CACCTGC 3 cut(s) 1814, 5306, 5488
PceI AGGCCT 1 cut(s) 4906
PciSI GCTCTTC 2 cut(s) 21, 1337
PcsI WCGNNNNNNNCGW 1 cut(s) 5977
PctI GAATGC 2 cut(s) 2382, 4758
PdiI GCCGGC 1 cut(s) 595
PdmI GAANNNNTTC 2 cut(s) 3124, 4756
PflMI CCANNNNNTGG 1 cut(s) 2951
PinAI ACCGGT 1 cut(s) 2229
PleI GAGTC 6 cut(s) 1430, 3013, 3489, 3842, 4420, 5576
PpsI GAGTC 6 cut(s) 1430, 3013, 3489, 3842, 4420, 5576
PpuMI RGGWCCY 3 cut(s) 329, 1961, 2628
PshBI ATTAAT 2 cut(s) 978, 4743
Psp124BI GAGCTC 1 cut(s) 71
Psp5II RGGWCCY 3 cut(s) 329, 1961, 2628
Psp6I CCWGG 5 cut(s) 1232, 4700, 5573, 5663, 6112
PspFI CCCAGC 3 cut(s) 2157, 2910, 4996
PspGI CCWGG 5 cut(s) 1232, 4700, 5573, 5663, 6112
PspPPI RGGWCCY 3 cut(s) 329, 1961, 2628
PspXI VCTCGAGB 1 cut(s) 5970
PsrI GAACNNNNNNTAC 2 cut(s) 1060, 1092
PstI CTGCAG 4 cut(s) 1969, 3766, 3781, 3891
PstNI CAGNNNCTG 5 cut(s) 509, 1035, 1964, 3951, 5900
PvuII CAGCTG 9 cut(s) 149, 506, 2273, 2507, 3155, 3767, 3776, 4490, 4502
RseI CAYNNNNRTG 3 cut(s) 3182, 4007, 6092
SacI GAGCTC 1 cut(s) 71
SapI GCTCTTC 2 cut(s) 21, 1337
SbfI CCTGCAGG 1 cut(s) 1969
ScaI AGTACT 1 cut(s) 757
SchI GAGTC 6 cut(s) 1430, 3013, 3489, 3843, 4421, 5576
ScrFI CCNGG 7 cut(s) 1234, 2758, 4702, 5169, 5575, 5665, 6114
SdaI CCTGCAGG 1 cut(s) 1969
SduI GDGCHC 7 cut(s) 71, 651, 1805, 2408, 3358, 3886, 5344
SfcI CTRYAG 7 cut(s) 238, 1965, 2790, 3762, 3777, 3887, 4368
Sfr274I CTCGAG 2 cut(s) 3825, 5970
SfuI TTCGAA 2 cut(s) 555, 952
SinI GGWCC 3 cut(s) 329, 1961, 2628
SlaI CTCGAG 2 cut(s) 3825, 5970
SmiMI CAYNNNNRTG 3 cut(s) 3182, 4007, 6092
SpeI ACTAGT 4 cut(s) 1282, 4193, 4508, 5189
SphI GCATGC 1 cut(s) 1890
Sse8387I CCTGCAGG 1 cut(s) 1969
SseBI AGGCCT 1 cut(s) 4906
SsiI CCGC 9 cut(s) 169, 784, 1682, 2958, 3041, 3310, 3469, 4364, 5104
SspI AATATT 3 cut(s) 3369, 4083, 4637
SstI GAGCTC 1 cut(s) 71
StuI AGGCCT 1 cut(s) 4906
StyD4I CCNGG 7 cut(s) 1232, 2756, 4700, 5167, 5573, 5663, 6112
StyI CCWWGG 9 cut(s) 213, 418, 871, 1141, 2076, 2528, 4718, 5543, 6165
TaiI ACGT 3 cut(s) 484, 3643, 5867
TaqII GACCGA 1 cut(s) 2465
TatI WGTACW 6 cut(s) 444, 755, 2148, 2197, 2572, 5446
TauI GCSGC 1 cut(s) 2960
TseFI GTSAC 4 cut(s) 893, 1271, 1409, 5516
Tsp45I GTSAC 4 cut(s) 893, 1271, 1409, 5516
TspGWI ACGGA 6 cut(s) 564, 1613, 1907, 5051, 5145, 6001
Van91I CCANNNNNTGG 1 cut(s) 2951
Vha464I CTTAAG 2 cut(s) 3247, 3526
VpaK11BI GGWCC 3 cut(s) 329, 1961, 2628
VspI ATTAAT 2 cut(s) 978, 4743
XagI CCTNNNNNAGG 2 cut(s) 773, 4699
XbaI TCTAGA 3 cut(s) 2321, 3919, 4559
XceI RCATGY 4 cut(s) 229, 543, 1890, 4313
XcmI CCANNNNNNNNNTGG 5 cut(s) 1566, 2182, 2211, 4025, 4490
XhoI CTCGAG 2 cut(s) 3825, 5970
XmaJI CCTAGG 1 cut(s) 4718
XmiI GTMKAC 1 cut(s) 5298
XmnI GAANNNNTTC 2 cut(s) 3124, 4756
ZrmI AGTACT 1 cut(s) 757
Zsp2I ATGCAT 3 cut(s) 2140, 4384, 4425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.