RchiOBHm_Chr5g0083811

Belongs to the peptidase C2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007783
Physical Location & Seq
Reverse (-)
89497659 .. 89514276
16618 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35792

Sequence Viewer

Length: 6450 bp
ATGGAAGGAGATGAGCGTCACGTGCTGCTCGCATGCGTCATTTCAGGAACCCTTTTCTCCGTTCTGGGTTCTGCTTCATTTTCTATACTCTGGCTTGTGAATTGGAGGCCGTGGAGGATCTACAGTTGGATATTCGCCAGGAAATGGCCAGATATCTTACACGGACCCCAGCTGGATATAGTATGTGGTTTTCTGTCACTCTCTGCATGGATTCTTGTCATTTCTCCAGTTCTTGTACTAATCGTATGGGGAAGCTGGTTGGTTGTAATATTGGATCGACATATAATTGGTCTGGCTGTTATTATGGCTGGTACTGCTCTTCTACTGGCTTTCTATTCAATCATGCTTTGGTGGCGAACGCAATGGCAGAGCTCAAGGGCTGTTGCTATTCTTCTTCTTCTTGCTGTTGCCCTTCTCTGTGCCTATGAACTCTGTGCTGTATATGTTACAGCTGGTTCAAAAGCATCTCAGCGGTATTCGCCTTCTGGTTTCTTTTTTGGTGTATCAGCAATTGCCTTGGCAATCAACATGCTCTTTATCTGTAGAATGGTGTTTAATGGAAATGGCTTAGATGTCGATGAGTATGTGAGGAAGGCATACAAATTTGCTTATTCTGATTGTATAGAAGTAGGTCCTGTGGCCTGTTTACCAGAACCACCAGATCCGAATGAGTTATATCCTCGACAATCTAGCAGGGCTTCACATCTTGGGCTTCTTTACCTTGGCTCACTTGTAGTTCTTCTCGTGTACTCAATCCTATATGGACTTACTGCAAAGGACGCACGTTGGCTTGGAGCCATTACATCAGCTGCTGTGATAATTCTTGATTGGAACATGGGAGCATGTTTATATGGTTTCGAACTCCTTAATAGTCGTGTAGCAGCATTATTTGTTGCCGGCACATCTCGAATTTTTCTCATTTGCTTCGGCGTTCACTACTGGTATTTGGGGCACTGCATTAGTTATGCAGTTGTAGCATCTGTTTTATTAGGTGCTTCTGTTTCTCGTCATTTATCTGTAACAAACCCATTGGCTGCAAGGAGAGATGCCTTACAGAGTACTGTTATTCGCCTCAGAGAGGGTTTCCGCAAAAAAGAACAGAACAGTTCATCAAGCTCGTCTGAAGGTTGTGGCTCAAGCATGAAACGCAGTAGTAGTGTTGAAGCAGGTTGCCTTGGTAATGTTGTTGAAGCAAGTCACAGGAGCGCAACACAGAGCACGGTTGATGCTAATAACTGGAGTAATGTTCTATTACGAACTGCGAGTTCTCACGAGGGGATTAATAGTGATAAAAGCATAGATAGTGGGAGGCCAAGTATAGCATTGTGTAGCAGTTCCTGTCGTTCAGTAATCCAAGAGCCTGAAGTGGGAACTTCCTTGGTGGTTTGTTCTAGCAGTGGTCTTGAAAGCCAAGGCTGTGAATCCAGTGCATCAAATTCTGCAAGTCAACAAACTTTAGATCTGAATTTAGCTTTTGCATTGCAAGAAAGGTTGAATGACCCCAGGATAACATCTATGCTAAAGAAGAGGGGAAGACAAAGTGACCTTGAACTAGTTAATCTTTTGCAGGATAAAGGACTGGATCCTAACTTTGTTATGATGTTGAAGGAGAAGAGCTTGGATCCAACCATTCTGGCATTACTTCAAAGAAGTAGTTTGGATGCTGATAGAGATCATCGTGACAATACAGATATCACCATAGCTGACTCAAACAGTGTGGATAATGGTTTACCCAATCAAATCTCTTTATCAGAAGAACTGAGGCTTCACGGGCTTGAAAAATGGCTCCAATTATCTAGGCTTGTTTTGCACCATGTAGTAGGTACCCCAGAGAGAGCATGGGTTCTCTTTAGTTTTGTTTTCATCCTCGAAACAATTGCTGTGGCAATTGTCCGTCCAAAGATAATCAAAATAATAAATGCGACACATCAACAGTTTGAATTTGGGTTTGCGGTGCTGCTGTTATCTCCTGTGGTCTGTTCAATCATGGCTTTTCTTCGATCTCTTCAAGCAGAAGAAATGGCCATGACCTCTAAGCCACGCAAGTATGGTTTTGTTGCTTGGCTGCTGAGCACCTGCGTTGGATTATTGCTTTCCTTCTTGAGCAAATCATCAGTTCTTCTGGGATTGTCTTTGACTGTTCCCCTCATGGTGGCATGCCTTTCTGTTGCCATTCCTACATGGATCCGTAATGGCTACCAATTTTGGGTTCCTCAATTACATTGTGCAGGTCCTGCAGGGAACCATCAAATTCGGGGAACAAAAGAGGGTGTTATTCTTGTCTTCTGCACAACATTATTTACTGGATCTGTATTAGCTCTCGGTGCAATAGTGTCTGCAAAGCCCTTGGATGACTTAAATTACAAAGGATGGACTGGTGAACAGAAAAGCTTTACATCTCCTTATGCATCATCTGTGTACATTGGCTGGGCTATGGCTTCTGCCATTGCTTTAGTGGTTACTGGTGTACTGCCAATTGTTTCATGGTTTGCATCTTACCGGTTCTCCATGTTTTCTGCTGTGTGTGTCGGGATATTTACAGCTGTCCTCGTGTCATTTTGTGGTGCATCCTATATTGAGGTTGTGAAATCTAGAGATGACCAAGTTCCAACAAAGGGTGATTTCCTTGCTGCGTTACTTCCTTTAATTTGCATTCCAGCATTTCTGTCTCTGTGCTCGGGGTTGTATAAATGGAAGGATGATAATTGGAAGCTTTCTCGGGGTGTCTATATCTTTGTAACCATCGGTCTTCTACTTCTACTCGGAGCAATATCAGCTGTTATAGTCGTAGTTACACCATGGACGGTTGGGGTATCATTTCTTTTAGTTCTTCTCATGATTGTACTGGCTATTGGTGCTATACATCATTGGGCCTCAAACAACTTTTATTTGACAAGGACCCAGACATTCTTTGTCTGTTTCCTCGCTTTTCTTTTGGCACTGGCTGCACTTCTTGTTGGATGGTTTGAAGATAAACCATTTGTCGGTGCTTCTGTCGGCTACTTTTTGTTTTTGTTTCTTCTTGCCGGGAGGGCATTGACTGTTCTTCTTTCACCTCCTATAGTTGTTTATTCTCCAAGAGTTCTCCCTGTTTATGTTTATGATGCTCATGCAGACTGTGCAAAGAATGTCAGTGCTGCATTTCTTGTGCTTTATGGGATTGCATTGGCAACTGAGGGCTGGGGTGTTGTTGCGAGTTTGAAAATTTATCCACCATTTGCTGGTGCCGCTGTATCAGCAATTACTCTTGTTGCTGCCTTTGGGTTTGCTTTCTCTCGTCCCTGCTTGACTCTCAAGATGATGGAAGATGCGGTACACTTTCTTAGTAAAGAAACCGTTGTTCAAGCTATTGCTAGATCTGCCACCAAGACCAGAAATGCTTTATCTGGAACTTATTCGGCTCCACAAAGGTCTGCCAGTTCAGCTGCTCTTTTGGTTGGAGATCCCACGATAATGCGTGATAGGGCAGGAAATTTTGTGCTTCCCAGAGCAGATGTCATGAAATTGAGAGATCGCTTAAGAAATGAGGAGTTAGTTGCAGGATCATTCTTCGGAAGAATGAGATATGGAAGGACTTTCCGCCATGAGCCAACAAGTAGTATTAATCACCGAAGAGAGATGTGTGCTCATGCACGAATATTGGCTTTGGAAGAGGCGATTGATACAGAATGGGTATATATGTGGGATAAATTTGGTGGTTATCTGCTTCTTTTGCTTGGTTTGACTGCTAAGGCAGAGCGAGTACAGGATGAGGTACGCTTGAGACTCTTTCTTGATAGCATAGGATTTGCAGACTTAAGTGCCAAGAAAATAAAAAAATGGATGCCAGAGGATCGTAGGCAGTTTGAGATTATTCAGGAGAGTTACATAAGAGAAAAGGAGATGGAAGAGGAACTCTTAATGCAAAGACGTGAAGAGGAGGGGAAAGGCAAAGAGAGAAGAAAGGCTCTCTTGGAGAAGGAAGAACGCAAATGGAAGGAGATAGAAGCGTCTTTAATTTCCTCCATTCCTAATGCTGGCAGCAGGGAAGCTGCAGCTGTGGCAGCTGCAGTGCGTGCAGTAGGGGGTGATTCTGTTCTTGATGATTCTTTTGCTCGAGAGAGAGTCTCAAGCATTGCGCGTCGCATACGCACAGCTCAGTTAACTCGGCGAGCACTGCAGACAGGAATTTCCGGAGCTGTATGCGTTCTTGATGATGAACCGACAACAAGTGGCAGGCACTGTGGCCAGATTGATTCTAGCATCTGTCAAAGTCAGAAGATCAGCTTTTCCATTGCAGTGATGATCCAACCTGTGTCTGGGCCTGTTTGCCTATTAGGCACTGAGTTTCAAAAGAAAGTTTGTTGGGAAATATTGGTCGCCGGTTCTGAGCAAGGAATTGAAGCTGGACAAGTTGGGCTTAGATTGATCACTAAAGGCGACAGACAAACTACTGTTGCAAAGGAGTGGAGTATTAGTGCAACTAGTATTGCAGATGGAAGGTGGCATTTGGTAACAATGACAATTGATGCTGATTTAGGTGAGGCAACTTGCTATCTCGATGGTGGTTTTGATGGCTACCAGACTGGTTTACCATTGCATGTGGGTAATACTATTTGGGAGCAAGGAACAGAAGTTTGGGTAGGTGTTAGACCGCCTACAGATATGGATGCATTTGGGAGGTCAGACAGTGAAGGGGCTGAGTCCAAGATGCATATTATGGATGTGTTTCTATGGGGAAGGTGCTTAACTGAGGATGATATTGCTGCTCTCCATACAGCTGTTGGGTCAGCTGATACTAGTATGATAGATTTTCCTGAAGATGCTTGGCAGTGGGCAGATTCACCTTCTAGACTTGATGAATGGGATAGTGATCCTGCCGATGTAGATCTATATGATAGGGATGAAGTTGATTGGGATGGGCAATATTCTAGTGGAAGGAAAAGAAGATCAGAGCGTGATGGAGTTCTGGTTGATTTGGATTCCTTTGCCAGGAGGTTTAGGAAACCTAGGATGGAAACGCAAGAAGAAATTAATCAACGAATGCTTTCTGTTGAATTGGCCGTCAAAGAAGCTCTCTGTGCAAGAGGAGAAACAAATTTTACTGATCAGGAGTTTCCTCCAAATGATCAATCACTATTTGTAGATTCGGAAAATCCCCCTTCAAAGTTGCAGGTCGTTTCAGAATGGATGAGGCCTGCTGATATTGTAAAAGAGAGCAGGCTTGCTACTCGGCCGTGCTTATTTTCTGGCACTGTGAATCCTTCTGATGTTTGTCAGGGTCGGCTGGGTGATTGTTGGTTTTTGAGTGCTGTTGCTGTTCTGACGGAAGTTTCTCGAATATCAGAAGTTATTATTACTCCAGAGTATAATGAGGAAGGAATCTACACTGTCCGCTTCTGTATTCAGGGTGAGTGGGTTCCTGTTGTTGTTGATGATTGGATTCCCTGTGAATCACCGGGTAAACCAGCATTTGCTACTAGTAGAAAAGGTAACGAGTTGTGGGTCTCTTTACTGGAGAAGGCATATGCCAAATTGCATGGCTCCTATGAAGCATTAGAAGGTGGACTTGTCCAAGATGCTCTTGTAGACCTCACTGGAGGTGCAGGTGAAGAAATTGATATGAGGAGTGCTCAGGCCCAAATTGATCTTGCGAGTGGTCGATTATGGTCTCAATTGTTGCGCTTCAAACAAGAGGGATTTCTTCTGGGTGCTGGAAGTCCATCTGGTTCAGATGTACATATTTCCTCCAGTGGCATTGTGCAAGGTCATGCCTACTCGTTATTGCAGGTGAGAGAGGTGGATGGTCACAAGCTTATTCAGATTCGCAATCCATGGGCCAATGAAGTTGAGTGGAATGGCCCCTGGTCTGACTCATCGCCTGAATGGACTGATAGGATGAAGCATAAGCTGAAGCATATTCCACAGTCAAAAGATGGCATATTCTGGATGTCCTGGCAAGACTTCCAAATTCATTTTCGATCAATATATGTTTGCCGTATCTATCCCCCTGAGATGCGCTACTCTGTTCATGGCCAATGGCGAAGTTATAGTGCTGGTGGCTGCCAAGACTATGAGACATGGCATCAAAATCCACAATTCCGATTGAGGGCCACAGGGCCAGATGCTTCATTTCCAATTCATGTATTCATTACGTTGACACAGGGTGTGAGCTTCTCAAGAACAGTTGCTGGTTTCAGAAACTATCAATCCAGTCATGATTCAATGATGTTCTATATTGGGATGAGGATACTCAAAACTCGAGGTCGCCGAGCTGCTTACAATATATACCTACATGAATCAGTCGGCGGGACAGATTATGTTAATTCACGAGAAATATCTTGTGAAATGGTTTTGGATCCAGATCCAAAAGGTTACACAATAGTGCCGACAACCATACACCCTGGCGAAGAAGCTCCATTTGTTCTTTCTGTTTTCACAAAAGCATCAATAACCTTGGAAGCCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001558 GO:0001708 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0004198 GO:0005198 GO:0005488 GO:0005509 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0005829 GO:0005886 GO:0005887 GO:0006275 GO:0006355 GO:0006508 GO:0006807 GO:0006873 GO:0006874 GO:0006875 GO:0006950 GO:0006970 GO:0006996 GO:0007010 GO:0007204 GO:0007275 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0008307 GO:0009628 GO:0009651 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009934 GO:0009966 GO:0009987 GO:0010008 GO:0010035 GO:0010038 GO:0010154 GO:0010468 GO:0010522 GO:0010524 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010646 GO:0010927 GO:0010941 GO:0010959 GO:0012505 GO:0014717 GO:0014718 GO:0014823 GO:0014850 GO:0016020 GO:0016021 GO:0016043 GO:0016787 GO:0019219 GO:0019222 GO:0019538 GO:0019725 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0030003 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030036 GO:0030154 GO:0030162 GO:0030234 GO:0030239 GO:0030315 GO:0031032 GO:0031090 GO:0031224 GO:0031226 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031402 GO:0031410 GO:0031420 GO:0031432 GO:0031674 GO:0031982 GO:0031984 GO:0032101 GO:0032268 GO:0032269 GO:0032270 GO:0032501 GO:0032502 GO:0032875 GO:0032877 GO:0032879 GO:0032947 GO:0032989 GO:0032991 GO:0033036 GO:0033233 GO:0033234 GO:0033554 GO:0034613 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0040008 GO:0042127 GO:0042175 GO:0042221 GO:0042383 GO:0042592 GO:0042692 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043269 GO:0043270 GO:0043292 GO:0043415 GO:0043416 GO:0043933 GO:0044085 GO:0044093 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044449 GO:0044459 GO:0044464 GO:0045165 GO:0045214 GO:0045740 GO:0045787 GO:0045862 GO:0045892 GO:0045893 GO:0045927 GO:0045934 GO:0045935 GO:0046872 GO:0048316 GO:0048468 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048583 GO:0048584 GO:0048608 GO:0048638 GO:0048639 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050801 GO:0050865 GO:0050867 GO:0050896 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051146 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051239 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051279 GO:0051281 GO:0051282 GO:0051480 GO:0051592 GO:0051641 GO:0051716 GO:0051726 GO:0051924 GO:0051928 GO:0055001 GO:0055002 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055103 GO:0060255 GO:0060341 GO:0060548 GO:0061041 GO:0061061 GO:0061458 GO:0065003 GO:0065007 GO:0065008 GO:0065009 GO:0070011 GO:0070727 GO:0070887 GO:0070925 GO:0071214 GO:0071241 GO:0071248 GO:0071277 GO:0071470 GO:0071472 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072657 GO:0080050 GO:0080090 GO:0080134 GO:0090068 GO:0090303 GO:0090329 GO:0090392 GO:0090627 GO:0090628 GO:0097264 GO:0097435 GO:0097708 GO:0098588 GO:0098771 GO:0098772 GO:0098805 GO:0098827 GO:0099080 GO:0099081 GO:0099512 GO:0104004 GO:0140096 GO:1901564 GO:1902531 GO:1902679 GO:1902680 GO:1903034 GO:1903036 GO:1903169 GO:1903320 GO:1903321 GO:1903506 GO:1903507 GO:1903508 GO:1904062 GO:1904064 GO:1904427 GO:2000011 GO:2000014 GO:2000024 GO:2000026 GO:2000105 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calpain_III Peptidase_C2

Protein Analysis

2149

Amino Acids

237.8

Weight (kDa)

6.13

Isoelectric Point (pI)

39.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C2 PF00648 1694 - 1993 4.5e-89 Calpain family cysteine protease
Calpain_III PF01067 2004 - 2139 1.1e-15 Calpain large subunit, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 2084, 5576, 5758
Acc36I ACCTGC 6 cut(s) 1157, 2084, 2219, 5143, 5576, 5758
Acc65I GGTACC 1 cut(s) 1823
AccB1I GGYRCC 2 cut(s) 1823, 3224
AccB7I CCANNNNNTGG 2 cut(s) 144, 3221
AccI GTMKAC 1 cut(s) 5568
AccII CGCG 1 cut(s) 4120
AccIII TCCGGA 1 cut(s) 4172
AciI CCGC 9 cut(s) 472, 1087, 1952, 3228, 3311, 3580, 4634, 5374, 6288
AcoI YGGCCR 6 cut(s) 146, 2022, 4225, 5040, 5213, 6013
AcuI CTGAAG 4 cut(s) 1143, 1383, 4818, 5912
AcvI CACGTG 1 cut(s) 22
AdeI CACNNNGTG 1 cut(s) 6146
AflII CTTAAG 2 cut(s) 3517, 3796
AgeI ACCGGT 1 cut(s) 2499
AhlI ACTAGT 4 cut(s) 1552, 4463, 4778, 5459
AjiI CACGTC 1 cut(s) 3911
AjnI CCWGG 6 cut(s) 137, 1502, 4970, 5843, 5933, 6382
AjuI GAANNNNNNNTTGG 6 cut(s) 2193, 2225, 4600, 4632, 5940, 5972
AleI CACNNNNGTG 1 cut(s) 6362
Alw21I GWGCWC 7 cut(s) 374, 1220, 2075, 2678, 3628, 4156, 5614
Alw26I GTCTC 6 cut(s) 2673, 3757, 4111, 5491, 5655, 6050
AlwNI CAGNNNCTG 5 cut(s) 812, 1338, 2234, 4221, 6170
Ama87I CYCGRG 4 cut(s) 2677, 2718, 4095, 6240
Aor13HI TCCGGA 1 cut(s) 4172
AseI ATTAAT 3 cut(s) 1281, 3603, 5013
AsiGI ACCGGT 1 cut(s) 2499
Asp700I GAANNNNTTC 2 cut(s) 3394, 5026
Asp718I GGTACC 1 cut(s) 1823
AspA2I CCTAGG 1 cut(s) 4988
AspLEI GCGC 4 cut(s) 1208, 4120, 5664, 6000
AsuC2I CCSGG 2 cut(s) 3028, 5439
AsuII TTCGAA 1 cut(s) 858
AvaI CYCGRG 4 cut(s) 2677, 2718, 4095, 6240
AvaII GGWCC 4 cut(s) 164, 632, 2231, 2898
AvrII CCTAGG 1 cut(s) 4988
BaeGI GKGCMC 1 cut(s) 954
BalI TGGCCA 4 cut(s) 148, 2024, 4227, 6015
BamHI GGATCC 4 cut(s) 1582, 1621, 2184, 6337
BanI GGYRCC 2 cut(s) 1823, 3224
BanII GRGCYC 1 cut(s) 374
BarI GAAGNNNNNNTAC 2 cut(s) 3297, 3329
BauI CACGAG 4 cut(s) 743, 1271, 2549, 6309
BbrPI CACGTG 1 cut(s) 22
BbsI GAAGAC 3 cut(s) 1540, 2275, 2741
Bbv12I GWGCWC 7 cut(s) 374, 1220, 2075, 2678, 3628, 4156, 5614
BceAI ACGGC 4 cut(s) 94, 5027, 5200, 5961
BcgI CGANNNNNNTGC 2 cut(s) 1859, 1893
BciT130I CCWGG 6 cut(s) 139, 1504, 4972, 5845, 5935, 6384
BciVI GTATCC 1 cut(s) 6222
BclI TGATCA 3 cut(s) 4407, 5086, 5107
BcnI CCSGG 2 cut(s) 3028, 5439
BcoDI GTCTC 6 cut(s) 2673, 3757, 4111, 5491, 5655, 6050
BcuI ACTAGT 4 cut(s) 1552, 4463, 4778, 5459
BfmI CTRYAG 8 cut(s) 121, 541, 2235, 3060, 4032, 4047, 4157, 4638
BfrI CTTAAG 2 cut(s) 3517, 3796
BfuAI ACCTGC 6 cut(s) 1157, 2084, 2219, 5143, 5576, 5758
BfuI GTATCC 1 cut(s) 6222
BglI GCCNNNNNGGC 2 cut(s) 3032, 4317
BglII AGATCT 3 cut(s) 1459, 3356, 4867
BlnI CCTAGG 1 cut(s) 4988
BlpI GCTNAGC 1 cut(s) 2069
BmcAI AGTACT 1 cut(s) 1060
Bme1390I CCNGG 8 cut(s) 139, 1504, 3028, 4972, 5439, 5845, 5935, 6384
Bme18I GGWCC 4 cut(s) 164, 632, 2231, 2898
BmeT110I CYCGRG 4 cut(s) 2677, 2718, 4095, 6240
BmgBI CACGTC 1 cut(s) 3911
BmrFI CCNGG 8 cut(s) 139, 1504, 3028, 4972, 5439, 5845, 5935, 6384
BpiI GAAGAC 3 cut(s) 1540, 2275, 2741
BplI GAGNNNNNCTC 4 cut(s) 4091, 4123, 5596, 5628
BpmI CTGGAG 6 cut(s) 210, 1258, 5325, 5516, 5598, 5713
Bpu10I CCTNAGC 2 cut(s) 3729, 5613
Bpu1102I GCTNAGC 1 cut(s) 2069
Bpu14I TTCGAA 1 cut(s) 858
BpuEI CTTGAG 7 cut(s) 358, 1120, 2122, 3278, 3781, 4093, 6142
BpuMI CCSGG 2 cut(s) 3028, 5439
BsaAI YACGTR 1 cut(s) 22
BsaBI GATNNNNATC 4 cut(s) 1671, 4851, 4866, 6342
BsaI GGTCTC 2 cut(s) 5491, 5655
BsaWI WCCGGW 2 cut(s) 2499, 4172
BsaXI ACNNNNNCTCC 8 cut(s) 1300, 1330, 2489, 2519, 4441, 4471, 4935, 4965
Bse118I RCCGGY 3 cut(s) 896, 2499, 4361
Bse3DI GCAATG 6 cut(s) 368, 1478, 2445, 4113, 4272, 4574
Bse8I GATNNNNATC 4 cut(s) 1671, 4851, 4866, 6342
BseAI TCCGGA 1 cut(s) 4172
BseBI CCWGG 6 cut(s) 139, 1504, 4972, 5845, 5935, 6384
BseJI GATNNNNATC 4 cut(s) 1671, 4851, 4866, 6342
BseMI GCAATG 6 cut(s) 368, 1478, 2445, 4113, 4272, 4574
BseRI GAGGAG 4 cut(s) 3542, 3932, 5082, 5620
BseSI GKGCMC 1 cut(s) 954
BseX3I CGGCCG 1 cut(s) 5213
BseYI CCCAGC 4 cut(s) 168, 2427, 3180, 5266
BsgI GTGCAG 5 cut(s) 2247, 2272, 2931, 4077, 5604
Bsh1236I CGCG 1 cut(s) 4120
Bsh1285I CGRYCG 1 cut(s) 5216
BshNI GGYRCC 2 cut(s) 1823, 3224
BshTI ACCGGT 1 cut(s) 2499
BsiEI CGRYCG 1 cut(s) 5216
BsiHKAI GWGCWC 7 cut(s) 374, 1220, 2075, 2678, 3628, 4156, 5614
BsiHKCI CYCGRG 4 cut(s) 2677, 2718, 4095, 6240
BsiSI CCGG 6 cut(s) 897, 2500, 3027, 4173, 4362, 5438
BslFI GGGAC 2 cut(s) 3264, 6304
BsmAI GTCTC 6 cut(s) 2673, 3757, 4111, 5491, 5655, 6050
BsmFI GGGAC 2 cut(s) 3264, 6304
BsmI GAATGC 2 cut(s) 2652, 5028
Bso31I GGTCTC 2 cut(s) 5491, 5655
BsoBI CYCGRG 4 cut(s) 2677, 2718, 4095, 6240
Bsp119I TTCGAA 1 cut(s) 858
Bsp1286I GDGCHC 8 cut(s) 374, 954, 1220, 2075, 2678, 3628, 4156, 5614
Bsp13I TCCGGA 1 cut(s) 4172
Bsp1407I TGTACA 2 cut(s) 2418, 5716
Bsp1720I GCTNAGC 1 cut(s) 2069
Bsp19I CCATGG 2 cut(s) 2798, 5813
BspACI CCGC 9 cut(s) 472, 1087, 1952, 3228, 3311, 3580, 4634, 5374, 6288
BspEI TCCGGA 1 cut(s) 4172
BspFNI CGCG 1 cut(s) 4120
BspHI TCATGA 3 cut(s) 2835, 3498, 6196
BspMAI CTGCAG 4 cut(s) 2239, 4036, 4051, 4161
BspMI ACCTGC 6 cut(s) 1157, 2084, 2219, 5143, 5576, 5758
BspQI GCTCTTC 2 cut(s) 324, 1607
BspT104I TTCGAA 1 cut(s) 858
BspT107I GGYRCC 2 cut(s) 1823, 3224
BspTI CTTAAG 2 cut(s) 3517, 3796
BspTNI GGTCTC 2 cut(s) 5491, 5655
BsrDI GCAATG 6 cut(s) 368, 1478, 2445, 4113, 4272, 4574
BsrFI RCCGGY 3 cut(s) 896, 2499, 4361
BsrGI TGTACA 2 cut(s) 2418, 5716
BssAI RCCGGY 3 cut(s) 896, 2499, 4361
BssSI CACGAG 4 cut(s) 743, 1271, 2549, 6309
Bst2BI CACGAG 4 cut(s) 743, 1271, 2549, 6309
Bst2UI CCWGG 6 cut(s) 139, 1504, 4972, 5845, 5935, 6384
Bst6I CTCTTC 8 cut(s) 324, 1520, 1607, 2010, 3607, 3645, 3882, 3909
BstAFI CTTAAG 2 cut(s) 3517, 3796
BstAPI GCANNNNNTGC 4 cut(s) 2234, 2945, 3119, 4055
BstAUI TGTACA 2 cut(s) 2418, 5716
BstBAI YACGTR 1 cut(s) 22
BstBI TTCGAA 1 cut(s) 858
BstDSI CCRYGG 3 cut(s) 110, 2798, 5813
BstENI CCTNNNNNAGG 2 cut(s) 1076, 4969
BstFNI CGCG 1 cut(s) 4120
BstHHI GCGC 4 cut(s) 1208, 4120, 5664, 6000
BstMAI GTCTC 6 cut(s) 2673, 3757, 4111, 5491, 5655, 6050
BstMCI CGRYCG 1 cut(s) 5216
BstNI CCWGG 6 cut(s) 139, 1504, 4972, 5845, 5935, 6384
BstNSI RCATGY 5 cut(s) 36, 532, 846, 2160, 4583
BstSCI CCNGG 8 cut(s) 137, 1502, 3026, 4970, 5437, 5843, 5933, 6382
BstSFI CTRYAG 8 cut(s) 121, 541, 2235, 3060, 4032, 4047, 4157, 4638
BstSLI GKGCMC 1 cut(s) 954
BstUI CGCG 1 cut(s) 4120
BstV2I GAAGAC 3 cut(s) 1540, 2275, 2741
BstZI CGGCCG 1 cut(s) 5213
BsuI GTATCC 1 cut(s) 6222
BtgI CCRYGG 3 cut(s) 110, 2798, 5813
BtgZI GCGATG 1 cut(s) 5841
BtrI CACGTC 1 cut(s) 3911
BtsI GCAGTG 6 cut(s) 952, 1402, 4056, 4154, 4284, 4817
BveI ACCTGC 6 cut(s) 1157, 2084, 2219, 5143, 5576, 5758
CaiI CAGNNNCTG 5 cut(s) 812, 1338, 2234, 4221, 6170
CciI TCATGA 3 cut(s) 2835, 3498, 6196
CfoI GCGC 4 cut(s) 1208, 4120, 5664, 6000
Cfr10I RCCGGY 3 cut(s) 896, 2499, 4361
CseI GACGC 5 cut(s) 5, 25, 788, 3978, 4109
CspAI ACCGGT 1 cut(s) 2499
CspCI CAANNNNNGTGG 4 cut(s) 1698, 1733, 1863, 1898
DraIII CACNNNGTG 1 cut(s) 6146
EaeI YGGCCR 6 cut(s) 146, 2022, 4225, 5040, 5213, 6013
EagI CGGCCG 1 cut(s) 5213
Eam1104I CTCTTC 8 cut(s) 324, 1520, 1607, 2010, 3607, 3645, 3882, 3909
EarI CTCTTC 8 cut(s) 324, 1520, 1607, 2010, 3607, 3645, 3882, 3909
EciI GGCGGA 1 cut(s) 3569
Ecl136II GAGCTC 1 cut(s) 372
EclXI CGGCCG 1 cut(s) 5213
Eco147I AGGCCT 1 cut(s) 5176
Eco24I GRGCYC 1 cut(s) 374
Eco31I GGTCTC 2 cut(s) 5491, 5655
Eco32I GATATC 2 cut(s) 154, 1693
Eco47I GGWCC 4 cut(s) 164, 632, 2231, 2898
Eco52I CGGCCG 1 cut(s) 5213
Eco53kI GAGCTC 1 cut(s) 372
Eco57I CTGAAG 4 cut(s) 1143, 1383, 4818, 5912
Eco72I CACGTG 1 cut(s) 22
Eco88I CYCGRG 4 cut(s) 2677, 2718, 4095, 6240
EcoICRI GAGCTC 1 cut(s) 372
EcoNI CCTNNNNNAGG 2 cut(s) 1076, 4969
EcoO109I RGGNCCY 3 cut(s) 632, 2231, 2898
EcoRII CCWGG 6 cut(s) 137, 1502, 4970, 5843, 5933, 6382
EcoRV GATATC 2 cut(s) 154, 1693
EcoT22I ATGCAT 3 cut(s) 2410, 4654, 4695
EcoT38I GRGCYC 1 cut(s) 374
FaqI GGGAC 2 cut(s) 3264, 6304
FauI CCCGC 1 cut(s) 6281
FauNDI CATATG 1 cut(s) 5506
FbaI TGATCA 3 cut(s) 4407, 5086, 5107
FblI GTMKAC 1 cut(s) 5568
FriOI GRGCYC 1 cut(s) 374
GlaI GCGC 4 cut(s) 1207, 4119, 5663, 5999
GsaI CCCAGC 4 cut(s) 172, 2431, 3184, 5270
GsuI CTGGAG 6 cut(s) 210, 1258, 5325, 5516, 5598, 5713
HapII CCGG 6 cut(s) 897, 2500, 3027, 4173, 4362, 5438
HgaI GACGC 5 cut(s) 5, 25, 788, 3978, 4109
HhaI GCGC 4 cut(s) 1208, 4120, 5664, 6000
Hin6I GCGC 4 cut(s) 1206, 4118, 5662, 5998
HinP1I GCGC 4 cut(s) 1206, 4118, 5662, 5998
HincII GTYRAC 3 cut(s) 1448, 4143, 6138
HindII GTYRAC 3 cut(s) 1448, 4143, 6138
HindIII AAGCTT 3 cut(s) 2389, 2711, 5792
HpaI GTTAAC 1 cut(s) 4143
HpaII CCGG 6 cut(s) 897, 2500, 3027, 4173, 4362, 5438
Hpy99I CGWCG 1 cut(s) 4125
HpyCH4IV ACGT 4 cut(s) 21, 784, 3910, 6134
HpySE526I ACGT 4 cut(s) 21, 784, 3910, 6134
HspAI GCGC 4 cut(s) 1206, 4118, 5662, 5998
Kpn2I TCCGGA 1 cut(s) 4172
KpnI GGTACC 1 cut(s) 1827
KroI GCCGGC 1 cut(s) 896
KroNI GCCGGC 1 cut(s) 898
Ksp22I TGATCA 3 cut(s) 4407, 5086, 5107
KspAI GTTAAC 1 cut(s) 4143
LguI GCTCTTC 2 cut(s) 324, 1607
MaeII ACGT 4 cut(s) 21, 784, 3910, 6134
MfeI CAATTG 6 cut(s) 510, 1875, 1887, 2475, 4503, 5654
MhlI GDGCHC 8 cut(s) 374, 954, 1220, 2075, 2678, 3628, 4156, 5614
MlsI TGGCCA 4 cut(s) 148, 2024, 4227, 6015
MluNI TGGCCA 4 cut(s) 148, 2024, 4227, 6015
MlyI GAGTC 6 cut(s) 1700, 3283, 3759, 4113, 4691, 5846
MmeI TCCRAC 7 cut(s) 107, 1649, 2062, 2633, 2938, 3418, 4312
Mox20I TGGCCA 4 cut(s) 148, 2024, 4227, 6015
Mph1103I ATGCAT 3 cut(s) 2410, 4654, 4695
MroI TCCGGA 1 cut(s) 4172
MroNI GCCGGC 1 cut(s) 896
MroXI GAANNNNTTC 2 cut(s) 3394, 5026
MscI TGGCCA 4 cut(s) 148, 2024, 4227, 6015
MslI CAYNNNNRTG 3 cut(s) 3452, 4277, 6362
Msp20I TGGCCA 4 cut(s) 148, 2024, 4227, 6015
MspCI CTTAAG 2 cut(s) 3517, 3796
MspI CCGG 6 cut(s) 897, 2500, 3027, 4173, 4362, 5438
MspR9I CCNGG 8 cut(s) 139, 1504, 3028, 4972, 5439, 5845, 5935, 6384
MunI CAATTG 6 cut(s) 510, 1875, 1887, 2475, 4503, 5654
Mva1269I GAATGC 2 cut(s) 2652, 5028
MvaI CCWGG 6 cut(s) 139, 1504, 4972, 5845, 5935, 6384
MvnI CGCG 1 cut(s) 4120
NaeI GCCGGC 1 cut(s) 898
NciI CCSGG 2 cut(s) 3028, 5439
NcoI CCATGG 2 cut(s) 2798, 5813
NdeI CATATG 1 cut(s) 5506
NgoMIV GCCGGC 1 cut(s) 896
NmeAIII GCCGAG 3 cut(s) 4126, 5191, 6275
NmuCI GTSAC 6 cut(s) 17, 195, 1196, 1541, 1679, 5786
NsiI ATGCAT 3 cut(s) 2410, 4654, 4695
NspI RCATGY 5 cut(s) 36, 532, 846, 2160, 4583
NspV TTCGAA 1 cut(s) 858
OliI CACNNNNGTG 1 cut(s) 6362
PaeI GCATGC 2 cut(s) 36, 2160
PaeR7I CTCGAG 2 cut(s) 4095, 6240
PagI TCATGA 3 cut(s) 2835, 3498, 6196
PaqCI CACCTGC 3 cut(s) 2084, 5576, 5758
PceI AGGCCT 1 cut(s) 5176
PciSI GCTCTTC 2 cut(s) 324, 1607
PcsI WCGNNNNNNNCGW 1 cut(s) 6247
PctI GAATGC 2 cut(s) 2652, 5028
PdiI GCCGGC 1 cut(s) 898
PdmI GAANNNNTTC 2 cut(s) 3394, 5026
PflMI CCANNNNNTGG 2 cut(s) 144, 3221
PinAI ACCGGT 1 cut(s) 2499
PleI GAGTC 6 cut(s) 1700, 3283, 3759, 4112, 4690, 5846
PmaCI CACGTG 1 cut(s) 22
PmlI CACGTG 1 cut(s) 22
PpsI GAGTC 6 cut(s) 1700, 3283, 3759, 4112, 4690, 5846
Ppu21I YACGTR 1 cut(s) 22
PpuMI RGGWCCY 3 cut(s) 632, 2231, 2898
PshBI ATTAAT 3 cut(s) 1281, 3603, 5013
Psp124BI GAGCTC 1 cut(s) 374
Psp5II RGGWCCY 3 cut(s) 632, 2231, 2898
Psp6I CCWGG 6 cut(s) 137, 1502, 4970, 5843, 5933, 6382
PspCI CACGTG 1 cut(s) 22
PspFI CCCAGC 4 cut(s) 168, 2427, 3180, 5266
PspGI CCWGG 6 cut(s) 137, 1502, 4970, 5843, 5933, 6382
PspPPI RGGWCCY 3 cut(s) 632, 2231, 2898
PspXI VCTCGAGB 1 cut(s) 6240
PstI CTGCAG 4 cut(s) 2239, 4036, 4051, 4161
PstNI CAGNNNCTG 5 cut(s) 812, 1338, 2234, 4221, 6170
RseI CAYNNNNRTG 3 cut(s) 3452, 4277, 6362
SacI GAGCTC 1 cut(s) 374
SapI GCTCTTC 2 cut(s) 324, 1607
SbfI CCTGCAGG 1 cut(s) 2239
ScaI AGTACT 1 cut(s) 1060
SchI GAGTC 6 cut(s) 1700, 3283, 3759, 4113, 4691, 5846
ScrFI CCNGG 8 cut(s) 139, 1504, 3028, 4972, 5439, 5845, 5935, 6384
SdaI CCTGCAGG 1 cut(s) 2239
SduI GDGCHC 8 cut(s) 374, 954, 1220, 2075, 2678, 3628, 4156, 5614
SfcI CTRYAG 8 cut(s) 121, 541, 2235, 3060, 4032, 4047, 4157, 4638
Sfr274I CTCGAG 2 cut(s) 4095, 6240
SfuI TTCGAA 1 cut(s) 858
SinI GGWCC 4 cut(s) 164, 632, 2231, 2898
SlaI CTCGAG 2 cut(s) 4095, 6240
SmiMI CAYNNNNRTG 3 cut(s) 3452, 4277, 6362
SpeI ACTAGT 4 cut(s) 1552, 4463, 4778, 5459
SphI GCATGC 2 cut(s) 36, 2160
Sse8387I CCTGCAGG 1 cut(s) 2239
SseBI AGGCCT 1 cut(s) 5176
SsiI CCGC 9 cut(s) 472, 1087, 1952, 3228, 3311, 3580, 4634, 5374, 6288
SspI AATATT 4 cut(s) 270, 3639, 4353, 4907
SstI GAGCTC 1 cut(s) 374
StuI AGGCCT 1 cut(s) 5176
StyD4I CCNGG 8 cut(s) 137, 1502, 3026, 4970, 5437, 5843, 5933, 6382
TaiI ACGT 4 cut(s) 24, 787, 3913, 6137
TaqII GACCGA 1 cut(s) 2735
TatI WGTACW 8 cut(s) 235, 747, 1058, 2418, 2467, 2842, 3742, 5716
TauI GCSGC 1 cut(s) 3230
TseFI GTSAC 6 cut(s) 17, 195, 1196, 1541, 1679, 5786
Tsp45I GTSAC 6 cut(s) 17, 195, 1196, 1541, 1679, 5786
TspGWI ACGGA 5 cut(s) 49, 177, 1883, 2177, 5321
Van91I CCANNNNNTGG 2 cut(s) 144, 3221
Vha464I CTTAAG 2 cut(s) 3517, 3796
VpaK11BI GGWCC 4 cut(s) 164, 632, 2231, 2898
VspI ATTAAT 3 cut(s) 1281, 3603, 5013
XagI CCTNNNNNAGG 2 cut(s) 1076, 4969
XbaI TCTAGA 2 cut(s) 2591, 4829
XceI RCATGY 5 cut(s) 36, 532, 846, 2160, 4583
XcmI CCANNNNNNNNNTGG 5 cut(s) 1836, 2452, 2481, 4295, 4760
XhoI CTCGAG 2 cut(s) 4095, 6240
XmaJI CCTAGG 1 cut(s) 4988
XmiI GTMKAC 1 cut(s) 5568
XmnI GAANNNNTTC 2 cut(s) 3394, 5026
ZrmI AGTACT 1 cut(s) 1060
Zsp2I ATGCAT 3 cut(s) 2410, 4654, 4695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.