MD03G1003600.v1.1

Belongs to the peptidase C2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007783
Physical Location & Seq
Forward (+)
260470 .. 274849
14380 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1003600.v1.1.491

Sequence Viewer

Length: 6483 bp
ATGGAAGGGGATGAGCGTCACCTGCTGCTGGCATGCGTGATTTCGGGGACCCTTTTCTCGGTTTTGGGTTCTGCTTCATTTTCTATACTGTGGCTTGTGAATTGGAGGCCCTGGAGGATCTACAGTTGGATCTTTGCCAGGAAATGGCCGAGTATCTTCCATGGACCTCAGCTGGATTTAGTATGTGGCTTTCTGTCTCTTTCCGCATGGCTACTCGTTATTTCTCCAGTTCTAGTACTGATCATATGGGGAAGCTGGTTGGTTGTAATATTGGATCGACATATAGTTGGTCTGGCTGTAATAATGGCTGGCACTGCTCTTCTACTGTCTTTCTATTCAATCATGCTTTGGTGGCGAACACAATGGCAGAGCTCGAGGGCTGTTGCTATTCTTCTTCTTCTGGCTGTTGCCCTTCTATGTGTGTACGAACTTTGTGCTGTGTATGTTACAGCTGGTTCAAAAGCATCTCAGCAGTATTCGCCGTCTGGTTTCTTTTTTGGCGTCTCAGCCGTTGCCTTGGCAATCAACATGCTCTTTATTTGCAGAATGGTGTTTAATGGGAATGGCTTAGATGTCGATGAATATGTGAGGAAGGCATATAAGTTTGCTTATTCTGATTGTATAGAAGTAGGTCCTGTGGCATGTTTACCAGAACCACCAGACCCTAATGAATTATATCCTCGACAATTTAGCAGGGCTTCACATCTTGGGCTTCTTTACCTTGGCTCACTTGTAGTCCTTCTTGTGTACTCAATCCTCTATGGTTTGACTGCTAAGGAGTCACGTTGGCTTGGAGCCATTACATCAGCTGCTGTTATCATTCTTGATTGGAACATGGGTGCATGCCTGTATGGTTTTGAACTCCTTCAAAGTCGTGTTGCAGCGTTATTTGTTGCTGGAACATCTCGGATCTTTCTCATTTGCTTTGGGGTCCACTACTGGTATTTTGGGCATTGCATTAGTTATGCAGTTGTAGCGTCTGTTTTATTGGGTGCTTCTGTTTCTCGCCATTTATCTGCAACAAACCCATTGGTTGCAAGGAGAGATGCCTTACAGAGTACCGTGACACGCCTCAGAGAGGGATTTCGCAAAAAAGAGCAAAACAGTTCATCAAGCTCATCTGAAGGTTGTGGTTCAAGCATGAAGCACAGTAGTAGTGTTGAAGCAGGTTGCCTTGGTAGTGTGGTTGAAGCAAGTAATAGGAGCACAGCACAGTGCACACTTGATGCCAATAACTGGACTAACACTCTCTTACGAACTGCAAGTTCTCGTGAGGGGATTAATAGTGATAAGAGCATAGATAGTGGAAGGCCAAGTTTAGCATTGCGTAGCAGTTCTTGTCGTTCAGTAATCCAGGAGCCTGAAGTGGGAACATCCTATACTGACAAAATTTTTGATCACAATAACACACTGATGATTTGTTCTAGTAGTGGTCTGGAAAGCCAAGGCTGTGAATCTAGTACGTCAAATTCTGCAAACCAACGAACTTTGGATTTGAATTTAGCTTTTGCATTGCAAGAAAGGTTGAGTGACCCGAGGATTACATCTATGTTAAAGAAGAGGGCAAGACAAGGTGATCTTGAATTGGTTAATCTATTGCAGGATAAAGGATTGGATCCTAACTTTGCTATGATGTTAAAGGAGAAGAGCTTGGATCCAACTATTCTGGCATTACTTCAAAGAAGTAGCTTGGATGCTGATAGAGATCACCGTGACAATACAGATATCACCATAGTTGACTCAAATAGTGTTGATAATGGTTTACCCAATCAAATCTCTTTGTCTGAAGAGCTAAGGCTTCATGGGCTTGAGAAGTGGCTTCAGTTATCTAGACTTGTTTTGCATCATGTGGTAGGCACCCCAGAACGGGCATGGGTTCTCTTTAGTTTTGTTTTCATCCTAGAAACTATTGCTGTGGCAATTTTTCGTCCAAGGACAATTAAAATAATAAATGCCACACATCAACAGTTTGAATTTGGCTTTGCAGTGCTGCTGTTATCTCCTGTTGTCTGTTCAATCATGGCTTTCCTTCGATCTCTTCAAGCAGAGGAAATGGCCATGACCTCAAAACCCCGCAAGTATGGCTTTGTTGCTTGGCTGCTCAGTACTTCTGTTGGATTGCTGCTTTCCTTCTTAAGCAAATCATCGGTTCTTCTTGGATTGTCCTTGACTGTCCCACTCATGGTGGCATGCCTCTCTGTTGCTATTCCTATATGGATACGTAATGGCTACCAGTTTAGGGTTCCTCAATTACAATGTGCAGGTCCTGCAGGGAACCATCAAATTCGAGGAACAAAAGAGGGTGTTATTCTTGTCTTAAGTACAATATTATTTGCTGGATCTGTGTTAGCCCTTGGTGCGATAGTGTCTGCAAAGCCCTTAGATGACTTAAAATACAAGGGCTGGACTGGTGAACAGAAAAGCTTTACCTCTCCTTATGCGTCATCTGTGTACATTGGCTGGGCTATGGCTTCTGTCATTGCTTTGGTGGTCACTGGTCTACTGCCAATTGTTTCTTGGTTTGCAACATACCGGTTCTCCCTGTCTTCTGCAGTGTGTGTTGGGATATTCACAGCTGTCCTTGTGGCATTTTGTGGTGCATCCTATATGGAGGTTGTGAAATCTAGAGATGACCAAGTTCCAACAGAGGGTGATTTCCTCGCTGCTTTACTTCCTTTAATTTGCAGTCCTGCATTGCTGTCTCTTTGCTCTGGGTTACATAAATGGAAGGATGATGATTGGAAACTCTCTCGGGGTGTTTATATCTTTGTCACCATTGGGCTTCTTCTTCTGCTTGGAGCAATATCAGCTGTTATAGTTGTAGTTATACCATGGACAATTGGGGTAGCATTTCTTTTAGTTCTTCTCATGCTTGTACTAGCTATTGGTGCTATCCATCATTGGGCTTCAAACAATTTCTATTTAACAAGGACCCAGACATTCTTTGTTTGTTTCCTTGCTTTTTTGTTGGCATTGGCGGCGTTTCTTGTTGGATGGTTTGAAGATAAAGCATTTGTAGGTGCTTCTGTTGGTTATTTTCTGTTCTTATTTCTTCTCGCTGGGAGAGCATTGACTGTTCTTCTTTCACCTCCTATTGTTGTTTATTCTCCGAGAGTTCTCCCTGTCTATGTTTATGATGCTCATGCAGATTGTGGAAAGAATGTCAGTGCGACATTTCTATTGCTTTATGGGATTGCATTGGCAACTGAAGGCTGGGGTGTTGTTGCGAGTCTGAAAATTTATCCACCATTTGCCGGTGCCTCTGTGTCAGCACTCACTCTTGTTGTAGCCTTTGGGTTTGCTTTCTCTCGTCCCTGCTTGACTCTCAAGATGATGGAAGATGCAGTACACTTTCTTAGTAAGGAAACCGTTGTTCAAGCTATTGCTAGATCTGCTACCAAGACCAGAAATGCTTTGTCTGGAACTTATTCTGCTCCACAGAGGTCTGCCAGTTCAGCGGCTCTCTTGGTTGGAGATCCCACCATTACACGTGATAGGGCAGGAAATTTTGTGCTTCCCAGAGCAGATGTTGTGAAATTGAGAGATCGTTTAAGAAATGAGGAGTTAGTTGCAGGCTCATTCTTCTGCAGAAAGAGATATGGAAGAACTTTCCGCCATGAGCCAACTAATGATGTTAATCATAGAAGAGAGATGTGTGCTCATGCACGAATATTGGCTTTGGAAGAGGCGATTGATACTGAATGGGTGTATATGTGGGATAAATTTGGTGGGTATCTGCTTCTTTTGCTTGGTTTGACTGCTAAGGCGGAGCGAGTACAGGATGAGGTACGTTTGAGACTCTTTCTTGATAGCATAGGGTTTGCTGACCTAAGTGCCAAGAAAATAAAAAAGTGGATGCCAGAGGACCGCAGGCAGTTTGAGATTATTCAAGAGAGTTATATAAGAGAAAAAGAGATGGAAGAGGAGCTCTTAGTGCAAAGGCGCGAAGAGGAGGGCAAAGGCAAAGTGAGAAGAAAGGCTCTTCTGGAGAAGGAAGAACGCAAGTGGAAGGAGATAGAAGCTTCTCTGATTTCCTACATTCCTAATGCTGGCAACAGGGAAGCAGCAGCTATGGCAGCTGCAGTGCGTGCAGTAGGAGGTGATTGTGTTCTTGATAATTCTTTTGCTCGAGAGAGAGTTTCAAGCATTGCGCGTCGCATACGCACAGCTCAGTTGGCTCGTCGAGCAGTCCAGACCGGAATTTCTGGGGCTGTATGCGTTCTTGATGATGAGCCAACCACAAGTGGCAGACACTGTGGCCAAATTGATCCCACCATTTGTCAAAGTCAAAAGATTAGCTTCTCTGTTACAGTGATGATTCAACCTGTATCTGGGCCTGTTTGCTTATTTGGCACTGAGTTTCAAAAGAAAGATTGCTGGGAAATATTGGTGGCAGGTTCCGAGCAAGGTATTGAAGCTGGACAAGTTGGGCTTAGATTGATAACGAAAGGTGATAGACAAACTACTGTTGCAAAGGAGTGGAGTATTAGTGCAACTAGTATAGCAGATGGAAGGTGGCATTTGGTAACAATGACAATTGATGCTGATTTAGGTGAGGCAACTTGCTATCTGGATGGTGGTTTTGATGGCTACCAGACTGGTTTACCATTGCAAGTGGGTAATACCATTTGGGAAGAAGGAACCGAAGTCTGGGTTGGTGTTAGACCACCGACAGATGTGGATGCGTTTGGGAGGTCAGACAGTGAAGGGGCTGAGTCTAAGATGCATATTATGGATGTGTTCCTATGGGGAAGGTGCTTAACTGAAGATGATGTTGCTGCTCTTCATTCAGCTATTGGTACAGCTGATTCCGATATGATAGATTTTCCTGAAGATAATTGGCAGTGGGCAGATTCACCCTCTAGAGTTGATGAATGGGATAGTGATCCTGCAGATGTAGATCTGTATGATAGGGATGACGTTGATTGGGACGGGCAATATTCTAGTGGGAGGAAACGAAGGTCAGAACGTGATACAGTGCTGGTTGATGTGGATTCAATAGCCAGGAGGTTTCGGAAACCTAGGATGGAAACACAAGACGAAATCAACCAGCGAATGCTTTCAGTTGAATTGGCAGTCAAGGAAGCCCTCTCTGCAAGGGGAGAGATACATTTTACCGATCAGGAGTTTCCTCCAAATGATCAGTCATTATTTGTAGATCCGGAAAATCCCCCATCAAAGTTGCAGGTTGTTTCTGAATGGGTGAGGCCTGCTGATATTGTAAAAGAGAGTCGGCTAGATGCTCGTCCGTGCTTATTTTCTGGAACTGTCAATCCTTCTGATGTTTGTCAGGGCCGACTTGGTGATTGCTGGTTTTTGAGTGCTGTTGCTGTTCTGACGGAGGTTTCACGAATATCGGAAGTGATCATTACCCCAGAGTATAATGAGGAAGGAGTCTACACTGTTCGCTTCTGTATTCAGGGTGAGTGGGTTCCTGTTGTTGTTGATGATTGGATTCCATGTGAGTCACCGGGGAAACCAGCGTTTGCTACTAGTAGGAAAGGTAACGAGCTATGGGTTTCTTTAGTGGAGAAGGCGTATGCTAAATTGCATGGCTCCTATGAAGCATTAGAAGGTGGGCTTGTCCAAGATGCTCTTGTCGACCTTACTGGAGGTGCTGGTGAGGAGATTGACATGAGGAGTGCTCAGGCCCAAATTGATCTTGCGAGTGGTCGACTGTGGTCTCAATTGCTGCGTTTCAAACAAGAAGGCTTTCTTCTTGGTGCTGGCAGTCCATCAGGTTCAGATGTACATGTTTCCTCCAGTGGCATTGTGCAAGGTCATGCCTACTCGTTATTGCAGGTGAGAGAGGTGGATGGCCACAAGCTTATTCAGATTCGGAATCCATGGGCTAATGAAGTTGAGTGGAATGGCCCTTGGTCGGACTCATCACCTCTTTGGACTGATAGGATGAAGCATAAGTTGAAGCATGTTCCGCAGTCAAAAGACGGCATATTCTGGATGTCCTGGCAAGACTTCCAAATTCATTTTCGATCAATATATGTTTGTTGTATCTACCCCCCTGAGATGCGCTATTCTGTTCATGGTCAATGGCGAGGTTATAGTGCCGGGGGCTGCCAAGATTATGAGACATGGCATCAAAATCCACAGTTCCGATTGAGGGCCACTGGGCCAGATGCTTCCCTTCCAATTCATGTATTCATTACGTTGACACAGGGTGTGAGCTTCTCAAGAACAGTTGCTGGTTTCAGAAATTATCAATCCAGTCATGATTCAATGATGTTCTACATTGGGATGAGGATACTCAAAACTCGAGGCCGTCGCGCTGCTTACAATATATATCTACATGAATCAGTTGATGGGACAGATTATGTCAATTCCCGAGAAATATCTTGTGAAATTGTTTTGGATCCAGATCCAAAAGGTTACACAATAGTGCCTACAACTATACATCCAGGGGAAGAAGCTCCATTTGTTCTTTCTGTTTTCACAAAAGCATCAATTACCTTGGAAGCCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001558 GO:0001708 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0004198 GO:0005198 GO:0005488 GO:0005509 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0005829 GO:0005886 GO:0005887 GO:0006275 GO:0006355 GO:0006508 GO:0006807 GO:0006873 GO:0006874 GO:0006875 GO:0006950 GO:0006970 GO:0006996 GO:0007010 GO:0007204 GO:0007275 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0008307 GO:0009628 GO:0009651 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009934 GO:0009966 GO:0009987 GO:0010008 GO:0010035 GO:0010038 GO:0010154 GO:0010468 GO:0010522 GO:0010524 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010646 GO:0010927 GO:0010941 GO:0010959 GO:0012505 GO:0014717 GO:0014718 GO:0014823 GO:0014850 GO:0016020 GO:0016021 GO:0016043 GO:0016787 GO:0019219 GO:0019222 GO:0019538 GO:0019725 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0030003 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030036 GO:0030154 GO:0030162 GO:0030234 GO:0030239 GO:0030315 GO:0031032 GO:0031090 GO:0031224 GO:0031226 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031402 GO:0031410 GO:0031420 GO:0031432 GO:0031674 GO:0031982 GO:0031984 GO:0032101 GO:0032268 GO:0032269 GO:0032270 GO:0032501 GO:0032502 GO:0032875 GO:0032877 GO:0032879 GO:0032947 GO:0032989 GO:0032991 GO:0033036 GO:0033233 GO:0033234 GO:0033554 GO:0034613 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0040008 GO:0042127 GO:0042175 GO:0042221 GO:0042383 GO:0042592 GO:0042692 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043269 GO:0043270 GO:0043292 GO:0043415 GO:0043416 GO:0043933 GO:0044085 GO:0044093 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044449 GO:0044459 GO:0044464 GO:0045165 GO:0045214 GO:0045740 GO:0045787 GO:0045862 GO:0045892 GO:0045893 GO:0045927 GO:0045934 GO:0045935 GO:0046872 GO:0048316 GO:0048468 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048583 GO:0048584 GO:0048608 GO:0048638 GO:0048639 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050801 GO:0050865 GO:0050867 GO:0050896 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051146 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051239 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051279 GO:0051281 GO:0051282 GO:0051480 GO:0051592 GO:0051641 GO:0051716 GO:0051726 GO:0051924 GO:0051928 GO:0055001 GO:0055002 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055103 GO:0060255 GO:0060341 GO:0060548 GO:0061041 GO:0061061 GO:0061458 GO:0065003 GO:0065007 GO:0065008 GO:0065009 GO:0070011 GO:0070727 GO:0070887 GO:0070925 GO:0071214 GO:0071241 GO:0071248 GO:0071277 GO:0071470 GO:0071472 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072657 GO:0080050 GO:0080090 GO:0080134 GO:0090068 GO:0090303 GO:0090329 GO:0090392 GO:0090627 GO:0090628 GO:0097264 GO:0097435 GO:0097708 GO:0098588 GO:0098771 GO:0098772 GO:0098805 GO:0098827 GO:0099080 GO:0099081 GO:0099512 GO:0104004 GO:0140096 GO:1901564 GO:1902531 GO:1902679 GO:1902680 GO:1903034 GO:1903036 GO:1903169 GO:1903320 GO:1903321 GO:1903506 GO:1903507 GO:1903508 GO:1904062 GO:1904064 GO:1904427 GO:2000011 GO:2000014 GO:2000024 GO:2000026 GO:2000105 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calpain_III Peptidase_C2

Protein Analysis

2161

Amino Acids

239.38

Weight (kDa)

6.04

Isoelectric Point (pI)

39.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C2 PF00648 1705 - 2003 1.8e-87 Calpain family cysteine protease
Calpain_III PF01067 2015 - 2150 4.8e-16 Calpain large subunit, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 30, 5791
Acc36I ACCTGC 6 cut(s) 30, 1157, 2252, 4385, 5176, 5791
AccB1I GGYRCC 2 cut(s) 1856, 3257
AccB7I CCANNNNNTGG 2 cut(s) 144, 1236
AccI GTMKAC 4 cut(s) 2500, 5397, 5601, 5674
AccII CGCG 3 cut(s) 3945, 4153, 6285
AccIII TCCGGA 1 cut(s) 5161
AciI CCGC 8 cut(s) 204, 2074, 2978, 3458, 3613, 3767, 3868, 5936
AcoI YGGCCR 4 cut(s) 146, 2055, 4258, 5818
AcuI CTGAAG 7 cut(s) 1143, 1383, 1805, 1806, 3228, 4785, 4851
AcvI CACGTG 1 cut(s) 3491
AcyI GRCGYC 1 cut(s) 501
AdeI CACNNNGTG 1 cut(s) 6179
AflII CTTAAG 2 cut(s) 2134, 2317
AflIII ACRYGT 2 cut(s) 3488, 5752
AgeI ACCGGT 1 cut(s) 2532
AhlI ACTAGT 2 cut(s) 4496, 5492
AjnI CCWGG 6 cut(s) 110, 137, 1353, 5003, 5966, 6415
AjuI GAANNNNNNNTTGG 4 cut(s) 4638, 4670, 5973, 6005
AleI CACNNNNGTG 1 cut(s) 6395
AloI GAACNNNNNNTCC 2 cut(s) 5257, 5289
Alw21I GWGCWC 6 cut(s) 374, 1208, 1220, 3661, 3930, 5647
Alw26I GTCTC 6 cut(s) 201, 508, 2706, 3790, 5688, 6083
Alw44I GTGCAC 1 cut(s) 1216
AlwNI CAGNNNCTG 4 cut(s) 812, 2267, 4254, 6203
Ama87I CYCGRG 6 cut(s) 373, 1534, 2751, 4128, 6273, 6342
Aor13HI TCCGGA 1 cut(s) 5161
ApaLI GTGCAC 1 cut(s) 1216
ArsI GACNNNNNNTTYG 4 cut(s) 1376, 1408, 5129, 5161
AseI ATTAAT 1 cut(s) 1281
AsiGI ACCGGT 1 cut(s) 2532
Asp700I GAANNNNTTC 4 cut(s) 864, 3427, 5059, 5712
AspA2I CCTAGG 1 cut(s) 5021
AspLEI GCGC 4 cut(s) 3945, 4153, 6033, 6287
AsuC2I CCSGG 2 cut(s) 5472, 6070
AvaI CYCGRG 6 cut(s) 373, 1534, 2751, 4128, 6273, 6342
AvaII GGWCC 7 cut(s) 48, 164, 632, 931, 2264, 2931, 3865
AvrII CCTAGG 1 cut(s) 5021
BaeGI GKGCMC 1 cut(s) 1220
BalI TGGCCA 3 cut(s) 2057, 4260, 5820
BamHI GGATCC 3 cut(s) 1615, 1654, 6370
BanI GGYRCC 2 cut(s) 1856, 3257
BanII GRGCYC 2 cut(s) 374, 3930
BarI GAAGNNNNNNTAC 2 cut(s) 3330, 3362
BauI CACGAG 1 cut(s) 1269
BbrPI CACGTG 1 cut(s) 3491
BbsI GAAGAC 1 cut(s) 2538
Bbv12I GWGCWC 6 cut(s) 374, 1208, 1220, 3661, 3930, 5647
BbvCI CCTCAGC 1 cut(s) 168
BceAI ACGGC 4 cut(s) 466, 494, 5965, 6264
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BciT130I CCWGG 6 cut(s) 112, 139, 1355, 5005, 5968, 6417
BciVI GTATCC 2 cut(s) 2211, 6255
BclI TGATCA 4 cut(s) 240, 1396, 5140, 5364
BcnI CCSGG 2 cut(s) 5472, 6070
BcoDI GTCTC 6 cut(s) 201, 508, 2706, 3790, 5688, 6083
BcuI ACTAGT 2 cut(s) 4496, 5492
BfmI CTRYAG 6 cut(s) 121, 2268, 2550, 3586, 4080, 4890
BfrI CTTAAG 2 cut(s) 2134, 2317
BfuAI ACCTGC 6 cut(s) 30, 1157, 2252, 4385, 5176, 5791
BfuI GTATCC 2 cut(s) 2211, 6255
BglII AGATCT 2 cut(s) 3389, 4900
BlnI CCTAGG 1 cut(s) 5021
BmcAI AGTACT 2 cut(s) 237, 2107
Bme1390I CCNGG 8 cut(s) 112, 139, 1355, 5005, 5472, 5968, 6070, 6417
Bme18I GGWCC 7 cut(s) 48, 164, 632, 931, 2264, 2931, 3865
BmeT110I CYCGRG 6 cut(s) 373, 1534, 2751, 4128, 6273, 6342
BmrFI CCNGG 8 cut(s) 112, 139, 1355, 5005, 5472, 5968, 6070, 6417
BmrI ACTGGG 1 cut(s) 6138
BmuI ACTGGG 1 cut(s) 6138
BoxI GACNNNNGTC 1 cut(s) 5680
BpiI GAAGAC 1 cut(s) 2538
BplI GAGNNNNNCTC 2 cut(s) 5629, 5661
BpmI CTGGAG 5 cut(s) 133, 210, 4007, 5631, 5746
Bpu10I CCTNAGC 5 cut(s) 168, 774, 1793, 3762, 5646
BpuEI CTTGAG 3 cut(s) 1829, 3311, 6175
BpuMI CCSGG 2 cut(s) 5472, 6070
BsaAI YACGTR 2 cut(s) 2222, 3491
BsaBI GATNNNNATC 5 cut(s) 1704, 3636, 4884, 4899, 6375
BsaHI GRCGYC 1 cut(s) 501
BsaI GGTCTC 1 cut(s) 5688
BsaWI WCCGGW 3 cut(s) 2532, 4196, 5161
BsaXI ACNNNNNCTCC 6 cut(s) 2522, 2552, 4474, 4504, 5522, 5552
Bse118I RCCGGY 2 cut(s) 2532, 3254
Bse3DI GCAATG 7 cut(s) 952, 1322, 1511, 2478, 2693, 4146, 4607
Bse8I GATNNNNATC 5 cut(s) 1704, 3636, 4884, 4899, 6375
BseAI TCCGGA 1 cut(s) 5161
BseBI CCWGG 6 cut(s) 112, 139, 1355, 5005, 5968, 6417
BseJI GATNNNNATC 5 cut(s) 1704, 3636, 4884, 4899, 6375
BseMI GCAATG 7 cut(s) 952, 1322, 1511, 2478, 2693, 4146, 4607
BseRI GAGGAG 5 cut(s) 3575, 3938, 3965, 5639, 5653
BseSI GKGCMC 1 cut(s) 1220
BseYI CCCAGC 4 cut(s) 2460, 3059, 3213, 4377
BsgI GTGCAG 2 cut(s) 2280, 4110
Bsh1236I CGCG 3 cut(s) 3945, 4153, 6285
BshNI GGYRCC 2 cut(s) 1856, 3257
BshTI ACCGGT 1 cut(s) 2532
BsiHKAI GWGCWC 6 cut(s) 374, 1208, 1220, 3661, 3930, 5647
BsiHKCI CYCGRG 6 cut(s) 373, 1534, 2751, 4128, 6273, 6342
BsiSI CCGG 6 cut(s) 2533, 3255, 4197, 5162, 5471, 6069
BslFI GGGAC 5 cut(s) 61, 2159, 3297, 4943, 6337
BsmAI GTCTC 6 cut(s) 201, 508, 2706, 3790, 5688, 6083
BsmBI CGTCTC 1 cut(s) 508
BsmFI GGGAC 5 cut(s) 61, 2159, 3297, 4943, 6337
BsmI GAATGC 1 cut(s) 5061
Bso31I GGTCTC 1 cut(s) 5688
BsoBI CYCGRG 6 cut(s) 373, 1534, 2751, 4128, 6273, 6342
Bsp1286I GDGCHC 6 cut(s) 374, 1208, 1220, 3661, 3930, 5647
Bsp13I TCCGGA 1 cut(s) 5161
Bsp1407I TGTACA 2 cut(s) 2451, 5749
Bsp19I CCATGG 3 cut(s) 160, 2831, 5846
BspACI CCGC 8 cut(s) 204, 2074, 2978, 3458, 3613, 3767, 3868, 5936
BspEI TCCGGA 1 cut(s) 5161
BspFNI CGCG 3 cut(s) 3945, 4153, 6285
BspHI TCATGA 1 cut(s) 6229
BspMAI CTGCAG 5 cut(s) 2272, 2554, 3590, 4084, 4894
BspMI ACCTGC 6 cut(s) 30, 1157, 2252, 4385, 5176, 5791
BspQI GCTCTTC 5 cut(s) 324, 1640, 1782, 3987, 4788
BspT107I GGYRCC 2 cut(s) 1856, 3257
BspTI CTTAAG 2 cut(s) 2134, 2317
BspTNI GGTCTC 1 cut(s) 5688
BsrDI GCAATG 7 cut(s) 952, 1322, 1511, 2478, 2693, 4146, 4607
BsrFI RCCGGY 2 cut(s) 2532, 3254
BsrGI TGTACA 2 cut(s) 2451, 5749
BssAI RCCGGY 2 cut(s) 2532, 3254
BssNI GRCGYC 1 cut(s) 501
BssSI CACGAG 1 cut(s) 1269
Bst2BI CACGAG 1 cut(s) 1269
Bst2UI CCWGG 6 cut(s) 112, 139, 1355, 5005, 5968, 6417
BstACI GRCGYC 1 cut(s) 501
BstAFI CTTAAG 2 cut(s) 2134, 2317
BstAPI GCANNNNNTGC 2 cut(s) 2267, 4088
BstAUI TGTACA 2 cut(s) 2451, 5749
BstBAI YACGTR 2 cut(s) 2222, 3491
BstDSI CCRYGG 3 cut(s) 160, 2831, 5846
BstENI CCTNNNNNAGG 1 cut(s) 1076
BstFNI CGCG 3 cut(s) 3945, 4153, 6285
BstHHI GCGC 4 cut(s) 3945, 4153, 6033, 6287
BstMAI GTCTC 6 cut(s) 201, 508, 2706, 3790, 5688, 6083
BstNI CCWGG 6 cut(s) 112, 139, 1355, 5005, 5968, 6417
BstNSI RCATGY 7 cut(s) 36, 532, 645, 846, 2193, 5756, 5933
BstPAI GACNNNNGTC 1 cut(s) 5680
BstSCI CCNGG 8 cut(s) 110, 137, 1353, 5003, 5470, 5966, 6068, 6415
BstSFI CTRYAG 6 cut(s) 121, 2268, 2550, 3586, 4080, 4890
BstSLI GKGCMC 1 cut(s) 1220
BstSNI TACGTA 1 cut(s) 2222
BstUI CGCG 3 cut(s) 3945, 4153, 6285
BstV2I GAAGAC 1 cut(s) 2538
BsuI GTATCC 2 cut(s) 2211, 6255
BtgI CCRYGG 3 cut(s) 160, 2831, 5846
BtsI GCAGTG 5 cut(s) 312, 1992, 2559, 4089, 4850
BveI ACCTGC 6 cut(s) 30, 1157, 2252, 4385, 5176, 5791
CaiI CAGNNNCTG 4 cut(s) 812, 2267, 4254, 6203
CciI TCATGA 1 cut(s) 6229
CfoI GCGC 4 cut(s) 3945, 4153, 6033, 6287
Cfr10I RCCGGY 2 cut(s) 2532, 3254
CseI GACGC 5 cut(s) 5, 490, 966, 2430, 4142
CspAI ACCGGT 1 cut(s) 2532
DraIII CACNNNGTG 1 cut(s) 6179
EaeI YGGCCR 4 cut(s) 146, 2055, 4258, 5818
EciI GGCGGA 2 cut(s) 3602, 3782
Ecl136II GAGCTC 2 cut(s) 372, 3928
Eco105I TACGTA 1 cut(s) 2222
Eco147I AGGCCT 1 cut(s) 5209
Eco24I GRGCYC 2 cut(s) 374, 3930
Eco31I GGTCTC 1 cut(s) 5688
Eco32I GATATC 1 cut(s) 1726
Eco47I GGWCC 7 cut(s) 48, 164, 632, 931, 2264, 2931, 3865
Eco53kI GAGCTC 2 cut(s) 372, 3928
Eco57I CTGAAG 7 cut(s) 1143, 1383, 1805, 1806, 3228, 4785, 4851
Eco72I CACGTG 1 cut(s) 3491
Eco88I CYCGRG 6 cut(s) 373, 1534, 2751, 4128, 6273, 6342
EcoICRI GAGCTC 2 cut(s) 372, 3928
EcoNI CCTNNNNNAGG 1 cut(s) 1076
EcoO109I RGGNCCY 5 cut(s) 48, 108, 632, 2264, 2931
EcoRII CCWGG 6 cut(s) 110, 137, 1353, 5003, 5966, 6415
EcoRV GATATC 1 cut(s) 1726
EcoT22I ATGCAT 1 cut(s) 4728
EcoT38I GRGCYC 2 cut(s) 374, 3930
Esp3I CGTCTC 1 cut(s) 508
FaqI GGGAC 5 cut(s) 61, 2159, 3297, 4943, 6337
FauI CCCGC 1 cut(s) 2081
FauNDI CATATG 1 cut(s) 245
FbaI TGATCA 4 cut(s) 240, 1396, 5140, 5364
FblI GTMKAC 4 cut(s) 2500, 5397, 5601, 5674
FriOI GRGCYC 2 cut(s) 374, 3930
GlaI GCGC 4 cut(s) 3944, 4152, 6032, 6286
GsaI CCCAGC 4 cut(s) 2464, 3063, 3217, 4381
GsuI CTGGAG 5 cut(s) 133, 210, 4007, 5631, 5746
HapII CCGG 6 cut(s) 2533, 3255, 4197, 5162, 5471, 6069
HgaI GACGC 5 cut(s) 5, 490, 966, 2430, 4142
HhaI GCGC 4 cut(s) 3945, 4153, 6033, 6287
Hin1I GRCGYC 1 cut(s) 501
Hin6I GCGC 4 cut(s) 3943, 4151, 6031, 6285
HinP1I GCGC 4 cut(s) 3943, 4151, 6031, 6285
HincII GTYRAC 4 cut(s) 1738, 5602, 5675, 6171
HindII GTYRAC 4 cut(s) 1738, 5602, 5675, 6171
HindIII AAGCTT 3 cut(s) 2422, 4020, 5825
HpaII CCGG 6 cut(s) 2533, 3255, 4197, 5162, 5471, 6069
Hpy99I CGWCG 3 cut(s) 4158, 4185, 6285
HpyCH4IV ACGT 8 cut(s) 784, 1463, 2221, 3490, 3790, 4920, 4969, 6167
HpySE526I ACGT 8 cut(s) 784, 1463, 2221, 3490, 3790, 4920, 4969, 6167
Hsp92I GRCGYC 1 cut(s) 501
HspAI GCGC 4 cut(s) 3943, 4151, 6031, 6285
KflI GGGWCCC 1 cut(s) 48
Kpn2I TCCGGA 1 cut(s) 5161
Ksp22I TGATCA 4 cut(s) 240, 1396, 5140, 5364
LguI GCTCTTC 5 cut(s) 324, 1640, 1782, 3987, 4788
LmnI GCTCC 9 cut(s) 794, 1203, 1357, 2798, 3439, 3769, 3925, 5561, 6433
MaeII ACGT 8 cut(s) 784, 1463, 2221, 3490, 3790, 4920, 4969, 6167
MfeI CAATTG 4 cut(s) 2508, 2838, 4536, 5687
MhlI GDGCHC 6 cut(s) 374, 1208, 1220, 3661, 3930, 5647
MlsI TGGCCA 3 cut(s) 2057, 4260, 5820
MluNI TGGCCA 3 cut(s) 2057, 4260, 5820
MmeI TCCRAC 7 cut(s) 107, 1682, 2095, 2666, 2971, 3451, 5862
Mox20I TGGCCA 3 cut(s) 2057, 4260, 5820
Mph1103I ATGCAT 1 cut(s) 4728
MroI TCCGGA 1 cut(s) 5161
MroXI GAANNNNTTC 4 cut(s) 864, 3427, 5059, 5712
MscI TGGCCA 3 cut(s) 2057, 4260, 5820
MslI CAYNNNNRTG 3 cut(s) 1412, 6254, 6395
Msp20I TGGCCA 3 cut(s) 2057, 4260, 5820
MspA1I CMGCKG 8 cut(s) 172, 452, 809, 2576, 2810, 3458, 4079, 4805
MspCI CTTAAG 2 cut(s) 2134, 2317
MspI CCGG 6 cut(s) 2533, 3255, 4197, 5162, 5471, 6069
MspR9I CCNGG 8 cut(s) 112, 139, 1355, 5005, 5472, 5968, 6070, 6417
MunI CAATTG 4 cut(s) 2508, 2838, 4536, 5687
Mva1269I GAATGC 1 cut(s) 5061
MvaI CCWGG 6 cut(s) 112, 139, 1355, 5005, 5968, 6417
MvnI CGCG 3 cut(s) 3945, 4153, 6285
NciI CCSGG 2 cut(s) 5472, 6070
NcoI CCATGG 3 cut(s) 160, 2831, 5846
NdeI CATATG 1 cut(s) 245
NmeAIII GCCGAG 1 cut(s) 174
NmuCI GTSAC 8 cut(s) 17, 780, 1063, 1529, 1712, 2491, 2770, 5466
NsiI ATGCAT 1 cut(s) 4728
NspI RCATGY 7 cut(s) 36, 532, 645, 846, 2193, 5756, 5933
OliI CACNNNNGTG 1 cut(s) 6395
PaeI GCATGC 3 cut(s) 36, 846, 2193
PaeR7I CTCGAG 3 cut(s) 373, 4128, 6273
PagI TCATGA 1 cut(s) 6229
PaqCI CACCTGC 2 cut(s) 30, 5791
PceI AGGCCT 1 cut(s) 5209
PciI ACATGT 1 cut(s) 5752
PciSI GCTCTTC 5 cut(s) 324, 1640, 1782, 3987, 4788
PctI GAATGC 1 cut(s) 5061
PdmI GAANNNNTTC 4 cut(s) 864, 3427, 5059, 5712
PflMI CCANNNNNTGG 2 cut(s) 144, 1236
PfoI TCCNGGA 1 cut(s) 1353
PinAI ACCGGT 1 cut(s) 2532
PmaCI CACGTG 1 cut(s) 3491
PmlI CACGTG 1 cut(s) 3491
Ppu21I YACGTR 2 cut(s) 2222, 3491
PpuMI RGGWCCY 4 cut(s) 48, 632, 2264, 2931
PscI ACATGT 1 cut(s) 5752
PshAI GACNNNNGTC 1 cut(s) 5680
PshBI ATTAAT 1 cut(s) 1281
Psp124BI GAGCTC 2 cut(s) 374, 3930
Psp5II RGGWCCY 4 cut(s) 48, 632, 2264, 2931
Psp6I CCWGG 6 cut(s) 110, 137, 1353, 5003, 5966, 6415
PspCI CACGTG 1 cut(s) 3491
PspFI CCCAGC 4 cut(s) 2460, 3059, 3213, 4377
PspGI CCWGG 6 cut(s) 110, 137, 1353, 5003, 5966, 6415
PspPPI RGGWCCY 4 cut(s) 48, 632, 2264, 2931
PspXI VCTCGAGB 2 cut(s) 373, 6273
PsrI GAACNNNNNNTAC 2 cut(s) 1363, 1395
PstI CTGCAG 5 cut(s) 2272, 2554, 3590, 4084, 4894
PstNI CAGNNNCTG 4 cut(s) 812, 2267, 4254, 6203
PvuII CAGCTG 7 cut(s) 172, 452, 809, 2576, 2810, 4079, 4805
RseI CAYNNNNRTG 3 cut(s) 1412, 6254, 6395
SacI GAGCTC 2 cut(s) 374, 3930
SalI GTCGAC 2 cut(s) 5600, 5673
SapI GCTCTTC 5 cut(s) 324, 1640, 1782, 3987, 4788
SbfI CCTGCAGG 1 cut(s) 2272
ScaI AGTACT 2 cut(s) 237, 2107
ScrFI CCNGG 8 cut(s) 112, 139, 1355, 5005, 5472, 5968, 6070, 6417
SdaI CCTGCAGG 1 cut(s) 2272
SduI GDGCHC 6 cut(s) 374, 1208, 1220, 3661, 3930, 5647
SfcI CTRYAG 6 cut(s) 121, 2268, 2550, 3586, 4080, 4890
Sfr274I CTCGAG 3 cut(s) 373, 4128, 6273
SinI GGWCC 7 cut(s) 48, 164, 632, 931, 2264, 2931, 3865
SlaI CTCGAG 3 cut(s) 373, 4128, 6273
SmiMI CAYNNNNRTG 3 cut(s) 1412, 6254, 6395
SmlI CTYRAG 8 cut(s) 373, 1808, 2134, 2317, 3326, 4128, 6190, 6273
SmoI CTYRAG 8 cut(s) 373, 1808, 2134, 2317, 3326, 4128, 6190, 6273
SnaBI TACGTA 1 cut(s) 2222
SpeI ACTAGT 2 cut(s) 4496, 5492
SphI GCATGC 3 cut(s) 36, 846, 2193
Sse8387I CCTGCAGG 1 cut(s) 2272
SseBI AGGCCT 1 cut(s) 5209
SsiI CCGC 8 cut(s) 204, 2074, 2978, 3458, 3613, 3767, 3868, 5936
SspI AATATT 5 cut(s) 270, 2328, 3672, 4386, 4940
SstI GAGCTC 2 cut(s) 374, 3930
StuI AGGCCT 1 cut(s) 5209
StyD4I CCNGG 8 cut(s) 110, 137, 1353, 5003, 5470, 5966, 6068, 6415
TaiI ACGT 8 cut(s) 787, 1466, 2224, 3493, 3793, 4923, 4972, 6170
TatI WGTACW 9 cut(s) 235, 747, 2105, 2321, 2451, 2875, 3346, 3775, 5749
TauI GCSGC 2 cut(s) 2981, 3461
TseFI GTSAC 8 cut(s) 17, 780, 1063, 1529, 1712, 2491, 2770, 5466
Tsp45I GTSAC 8 cut(s) 17, 780, 1063, 1529, 1712, 2491, 2770, 5466
TspGWI ACGGA 2 cut(s) 5238, 5354
Van91I CCANNNNNTGG 2 cut(s) 144, 1236
Vha464I CTTAAG 2 cut(s) 2134, 2317
VneI GTGCAC 1 cut(s) 1216
VpaK11BI GGWCC 7 cut(s) 48, 164, 632, 931, 2264, 2931, 3865
VspI ATTAAT 1 cut(s) 1281
XagI CCTNNNNNAGG 1 cut(s) 1076
XbaI TCTAGA 3 cut(s) 1829, 2624, 4862
XceI RCATGY 7 cut(s) 36, 532, 645, 846, 2193, 5756, 5933
XcmI CCANNNNNNNNNTGG 2 cut(s) 1869, 2514
XhoI CTCGAG 3 cut(s) 373, 4128, 6273
XmaJI CCTAGG 1 cut(s) 5021
XmiI GTMKAC 4 cut(s) 2500, 5397, 5601, 5674
XmnI GAANNNNTTC 4 cut(s) 864, 3427, 5059, 5712
ZrmI AGTACT 2 cut(s) 237, 2107
Zsp2I ATGCAT 1 cut(s) 4728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.