MD11G1003500.v1.1

Belongs to the peptidase C2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007783
Physical Location & Seq
Forward (+)
281712 .. 297306
15595 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1003500.v1.1.491

Sequence Viewer

Length: 6483 bp
ATGGAAGGAGATGAGCGTCGCCTGCTGCTAGCATGCGTGATTTCTGGGACCCTTTTCTCCGTTTTGGGTTCTGCTTCATTTTCTATACTGTGGCTTGTGAATTGGAGGCCCTGGAGGATCTACAGTTGGATCTTTGCCAGGAAATGGCCAGGTATCTTCCATGGACCTCAGCTGGATATAGTATGTGGCTTTCTGTCTCTTTCCGCATGGATACTTGTCATTTCTCCGGTCCTAGTACTGATCATATGGGGAAGCTGGTTGGTTGTAATATTGGATCGACATATAGTTGGTCTGGCGGTAATAATGGCTGGCACTGCTCTCCTACTGTCTTTCTATTCGATCATGCTTTGGTGGCGAACGCAATGGCAGAGCTCAAGGGCTGTTGCAATTCTTCTTCTTCTGGCTGTTGCCCTTCTATGTGCGTACGAACTTTGTGCTGTGTATGTTACAGCTGGTTCAAAAGCATCTCAGCGGTATTCACCGTCTGGTTTCTTTTTTGGCGTGTCAGCAGTTGCCTTGGCAATCAACATGCTCTTTATTTGCAGAATGGTGTTTAATGGGAATGGCTTAGATGTTGATGAATATGTGAGGAAGGCATATAAATTTGCTTATTCTGATTGTATAGAAGTAGGTCCTGTGGCATGTTTACCAGAACCACCAGACCCTAATGAATTATATCCTCGACAATCTAGCAGGGCTTCACATCTTGGGCTTCTTTACCTTGGCTCACTTGTAGTCCTTCTTGTGTACTCAATCCTCTATGGTTTGACTGCAAAGGAGTCACGTTGGCTTGGAGCCATTACATCAGCTGCTGTCATCATTCTTGATTGGAACATGGGTGCATGCTTGTATGGATTCGAACTCCTTCAAAGTCGTGTTGCAGCATTATTTGTTGCTGGAACATCTCGGATCTTTCTCATTTGCTTTGGGGTCCACTACTGGTATTTTGGGCACTGCATTAGTTATGCAGTTGTAGCTTCTGTTTTATTGGGCGCTTCTGTTTCTCGTCATTTATCTGCAATGAACCCGTTGGCTGCAAGGAGAGATGCCTTACAGAGTACCGTGATTCGCCTCAGAGAGGGATTTCATAAAAAAGAGCAAAACAGTTCATCTAGCTCATCTGAAGGTTGTGGTTCAAGCATGAAGCGCAGTAGTAGTGTTGAAGCAGGTTGCCTGGGTAATGTGGTTGAAGCAAGCAATAGGAGCACAACACAGTGCACACTTGATGCCAATAACTGGACTAACACTCTATTACGAGCTGCAAGTTCTCGTGAGGGGATTAATAGTGATAAGAGCATCGATAGTGGAAGGCCAAGTTTAGCATTGCGTAGCAGTTCTTGTCGTTCAGTAATCCAAGAGCCTGAAGTGGGCACATCCTATACCGACAAAAATTTTGATCACAATAACACATTGATGGTTTGTTCTAGTAGTGGTCTGGAAAGCCAAGGCTGTGAATCTAGTACATCAAATTCTGCAAACCAACAAACTTTGGATTTGAACTTAGCTTTTGCATTGCAAGAAAGGTTGAGTGACCCGAGGATTACATCTATGTTAAAGAAGAGGGCAAGACAGGGTGATCTTGAATTGGTTAATCTATTGCAGGATAAAGGATTGGATCCTAACTTTGCTATGATGTTAAAGGAGAAGAGCTTGGATCCTACTATTCTGGCATTACTTCAAAGAAGTAGCTTGGACGCTGATAGAGATCACCGTGACAACACAGATATCACCGTAGTTGACTCAAATAGTGTTGGTCATGGTTTACCCAATCAAATCTCTTTGTCAGAAGAACTGAGGCTTCATGGGCTTGAGAAATGGCTTCAATTATCGAGACTTGTTTTGCATCATGTAGTAGGCACCCCAGAACGGGCATGGGTTCTCTTTAGTTTAGTTTTCATTCTAGAAACAATTGCTGTGGCAATTGTTCGTCCAAGGACAATAAAAATAATAAATGCCACACATCAACAGTTTGAATTTGGCTTTGCGGTGCTGCTGTTATCTCCTGTCGTCTGTTCAATCATGGCTTTCCTTCGATCTCTTCAAGCAGAGGATATGGCCATGACCTCAAAACCCCGCAAGTATGGCTTTGTTGCTTGGCTGCTCAGCACTTCTGTTGGATTGCTGCTTTCCTTCTTGAGCAAATCCTCAGTTCTTCTTGGATTGTCCTTGACTGTCCCACTCATGGTGGCATGCCTTTCTGTTGCAATTCCTATATGGATCCGTAATGGTTACCAGTTTAGGGTTCCTCAATTACAATGTGCAGGTCCTGCAGGAAACCATCAAATTCGTGGAACAAAAGAGGGTGTTATTCTTGTCTTAAGTACAATATTATTTGCCGGATCTGTGTTAGCTCTTGGTGCGATAGTGTCTGCAAAGCCCTTGGATGACTTAAAGTACAAAGGCTGGACTGGTGAACAGAAAAGCTTTAACTCTCCATATGCGTCATCCGTATACATTGGGTGGGCTATGGCTTCTGCGATTGCTTTGATGGTCACTGGTCTACTGCCGATTGTTTCATGGTTTGCAACATACCGGTTCTCCCTGTCTTCTGCAGTGTGTGTTGGGATATTCACAGTTGTCCTTGTGGCATTTTGTGGTGCATCCTATATGGAGGTTGTGAAATCTAGAGATGACCAAGTTCCAACAGAGGGTGATTTCCTTGCTGCTTTACTTCCTTTAATTTGCAGTCCTGCATTGTTGTCTCTTTGCTCTGGGTTACATAAATGGAAGGATGATGATTGGAAACTCTCTCGGGGTGTTTATATCTTTGTCACAATTGGGCTGCTGCTTCTGCTTGGGGCAATATCAGCTGTTATAGTTGTAGTTAAACCATGGACGGTTGGGGTGGCGTTTCTTTTAGTTCTTCTCATGATTGTACTAGCTATTGGTGCTATCCATCATTGGGCTTCAAACAATTTCTATTTGACAAGGACCCAGACATTCTTTGTTTGTTTCCTTGCATTTTTGTTGGCATTGGCAGCATTTCTTGTTGGATGGTTTGAAGATAAAGCATTTGTGGGTGCTTCAGTTGGTTATTTTCTATTCTTATTTCTTCTTGCTGGGAGAGCATTGACTGTTCTTCTTTCACCTCCTATTGTTGTTTATTCTCCAAGAGTTCTCCCAGTCTATGTTTATGATGCTCATGCAGATTGCGGAAAGAATGTCAGTATGGCATTTCTAGTGCTTTATGGGATTGCATTGGCAACTGAAGGCTGGGGTGTTGTTGCCAGTTTGAAAATTTATCCACCATTTGCTGGTGCCTCTGTGTCAGCAATCACTCTTGTTGTAGCCTTTGGGTTTGCTTTCTCTCGTCCCTGCTTGACTCTCAAGATGATGGAAGATGCAGTACACTTTCTCAGTAAGGAAACCGTTGTTCAAGCTATTGCTAGATCTGCTACCAAGACCAGAAATGCTTTGTCTGGAACTTATTCTGCTCCACAAAGGTCTGCTAGTTCAGCGGCTCTCTTGGTTGGAGATCCCACCATTATGCGTGATAGGGCAGGAAATTTTGTGCTTCCCAGAGCAGATGTCATGAAATTAAGAGATCGTTTAAGAAATGAGGAGTTAGTTGCAGGCTCATTCTTCTGCAGAAAGAGATATGGAAGAACTTTCCGCCATGAACCAACTAATGATGTTGATCATAGAAGAGAGATGTGTGCTCATGCACGAATATTGGCTTTGGAAGAGGCGATTGATACTGAATGGGTGTATATGTGGGATAAATTTGGTGGGTATCTGCTTCTTTTGCTTGGTTTGACTGCTAAGGCAGAGCGAGTACAGGATGAGGTACGTTTGAGACTCTTTCTCGATAGCATAGGGTTTGCTGACCTAAGTGCCAAGAAAATAAAAAAGTGGATGCCCGAGGACCGCAGGCGGTTTGAGATTATTCAAGAGAGTTATATAAGAGAAAAAGAGATGGAAGAGGAGCTCTTAATGCAAAGGCGCGAAGAGGAGGGGAAAGGCAAAGAGAGAAGAAAGGCTCTCCTGGAGAAGGAAGAACGCAAGTGGAAGGAGATAGAAGCTTCTCTGATTTCCTCCATTCCTAATGCTGGCAACAGGGAAGCAGCAGCTATGGCAGCTGCAGTGCGGGCAGTAGGGGGTGATTCTGTTCTTGATGATTCTTTTGCTCGAGAGAGAGTTTCAAGCATTGCGCGTCGCATACGAACAGCTCAGTTGGCTCGTCGAGCAGTTCAGACTGGAATTTCTGGGGCTGTATGTGTTCTAGATGATGAGCCTATAACAAGTGGCAGACACTGTGGCCAGATTCACCCCACCATTTGTCAAAGTCAAAAGATTAGCTTCTCCGTTGCAGTGATGATTCAACCTGTATCTGGGCCTGTTTGCTTATTTGGCACTGAGTTTCAAAAGAAAGTTTGCTGGGAAATATTGGTGGCCGGTTCTGAGCAAGGTATTGAAGCTGGACAAGTTGGGCTTAGATTGATCACAAAAGGTGATAGACAAACTACTGTTGCAAAGGAGTGGAGTATTAGTGCAACTAGTATAGCAGATGGAAGGTGGCATTTGGTAACAATGACGATTGATGCTGATTTAGGTGAGGCAACTTGCTATCTGGATGGTGGTTTTGATGGCTACCAGACCGGTTTACCATTGCATGTGGGTAATACTGTCTGGGAAGAAGGAACAGAAGTTTGGGTTGGTGTTAGACCACCTACAGATGTGGATGCGTTTGGGAGGTCAGACAGTGAAGGGGCTGAGTTTAAGATGCATATTATGGATGTGTTCCTATGGGGAAGGTGCTTAACTGAAGATGATGTTGCTGCTCTTCAGTCAGCTATTGGCTCAGCTGATTCTGATATGATAGATTTTCCTGAAGATAATTGGCAATGGGCAGATTCACCCTCTAGAGTTGATGAATGGGATAGTGATCCTGCAGATGTAGATCTATATGATAGGGATGATGTTGATTGGGATGGGCAATACTCCAGTGGGAGGAAACGAAGGGCAGAACGTGATGCAGTGCTGGTTGATGTGGATTCATTTGCCAGGAGGTTTCGGAAACCTAGGATGGAAACACAAGAAGAAATCAACCAGCGAATGCTTTCAGTTGAATTGGCAGTCAAGGAAGCCCTCTCTGCAAGGGGAGAAATACATTTTACTGATCAGGAGTTTCCTCCAAATGATCAGTCATTATTTGTAGATCCAGAAAATCCCCCATCAAAGTTGCAGGTTGTTTCTGAGTGGGTGAGGCCTGCTGATATTGTAAAAGAGAGTCGGCTGGATGCTCGTCCATGCTTATTTTCTGGCACTGTCAATCCTTCTGATGTTTGTCAGGGCCGACTTGGTGATTGCTGGTTTTTGAGTGCCGTTGCTGTTCTGACGGAGGTTTCACGAATATCGGAAGTGATAATTACCCCAGAGTATAATGAGGAAGGAATCTACACTGTTCGCTTCTGTATTCAGGGTGAGTGGGTTCCTGTTGTTGTGGATGATTGGATTCCATGTGAGTCACCGGGGAAACCAGCGTTTGCTACTAGTAGGAAAGGTAACGAGCTATGGGTTTCTTTATTGGAGAAGGCGTATGCTAAATTGCATGGCTCCTATGAAGCATTAGAAGGTGGGCTTGTCCAAGATGCTCTTGTCGACCTTACTGGAGGTGCTGGCGAGGAGATTGACATGAGGAGTGCGCAGGCCCAAATTGATCTTGCAAGTGGTCGACTATGGTCTCAATTGCTGCGCTTCAAACAAGAAGGCTTTCTTCTTGGTGCTGGCAGTCCATCAGGTTCAGATGTTCATGTTTCCTCCAGTGGCATTGTGCAAGGTCATGCCTACTCGTTATTGCAGGTGAGAGAGGTGGATGGCCACAAGCTTATTCAGATTCGGAATCCATGGGCTAATGAAGTTGAGTGGAATGGCCCTTGGTCAGACTCATCATCTCTTTGGACTGATAGGATGAAGCATAAGTTGAAGCATGTTCCTCAGTCAAAGGACGGCATATTCTGGATGTCCTGGCAAGACTTCCAAATTCATTTTCGATCAATATATGTTTGTCGTGTCTACCCCCCCGAGATGCGCTATTCTGTTCATGGCCAATGGCGAGGTTATAGTGCAGGTGGCTGCCAAGATTATGAGACATGGCATCAAAATCCACAGTTCCGACTGAGGGCCACTGGCCCCGATGCCGCGCTTCCAATTCACGTATTCATTACGTTGACACAGGGTGTGAGCTTCTCAAGAACGGTTGCTGGTTTCAGAAACTATCAATCCAGTCATGATTCAATGATGTTCTACATTGGGATGAGGATACTCAAAACTCGAGGCCGTCGTGCTGCTTACAATATATACCTACATGAATCAGTTGGTGGGACAGATTATGTCAACTCTCGAGAAATATCTTGTGAAATGGTTTTGGATCCAGATCCAAAAGGTTACACAATTGTGCCTACAACTATACATCCAGGGGAAGAAGCTCCATTTGTTCTTTCTGTTTTCACAAAAGCATCAATTACCTTGGAAGCCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001558 GO:0001708 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0004198 GO:0005198 GO:0005488 GO:0005509 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0005829 GO:0005886 GO:0005887 GO:0006275 GO:0006355 GO:0006508 GO:0006807 GO:0006873 GO:0006874 GO:0006875 GO:0006950 GO:0006970 GO:0006996 GO:0007010 GO:0007204 GO:0007275 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0008307 GO:0009628 GO:0009651 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009934 GO:0009966 GO:0009987 GO:0010008 GO:0010035 GO:0010038 GO:0010154 GO:0010468 GO:0010522 GO:0010524 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010646 GO:0010927 GO:0010941 GO:0010959 GO:0012505 GO:0014717 GO:0014718 GO:0014823 GO:0014850 GO:0016020 GO:0016021 GO:0016043 GO:0016787 GO:0019219 GO:0019222 GO:0019538 GO:0019725 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0030003 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030036 GO:0030154 GO:0030162 GO:0030234 GO:0030239 GO:0030315 GO:0031032 GO:0031090 GO:0031224 GO:0031226 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031402 GO:0031410 GO:0031420 GO:0031432 GO:0031674 GO:0031982 GO:0031984 GO:0032101 GO:0032268 GO:0032269 GO:0032270 GO:0032501 GO:0032502 GO:0032875 GO:0032877 GO:0032879 GO:0032947 GO:0032989 GO:0032991 GO:0033036 GO:0033233 GO:0033234 GO:0033554 GO:0034613 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0040008 GO:0042127 GO:0042175 GO:0042221 GO:0042383 GO:0042592 GO:0042692 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043269 GO:0043270 GO:0043292 GO:0043415 GO:0043416 GO:0043933 GO:0044085 GO:0044093 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044449 GO:0044459 GO:0044464 GO:0045165 GO:0045214 GO:0045740 GO:0045787 GO:0045862 GO:0045892 GO:0045893 GO:0045927 GO:0045934 GO:0045935 GO:0046872 GO:0048316 GO:0048468 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048583 GO:0048584 GO:0048608 GO:0048638 GO:0048639 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050801 GO:0050865 GO:0050867 GO:0050896 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051146 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051239 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051279 GO:0051281 GO:0051282 GO:0051480 GO:0051592 GO:0051641 GO:0051716 GO:0051726 GO:0051924 GO:0051928 GO:0055001 GO:0055002 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055103 GO:0060255 GO:0060341 GO:0060548 GO:0061041 GO:0061061 GO:0061458 GO:0065003 GO:0065007 GO:0065008 GO:0065009 GO:0070011 GO:0070727 GO:0070887 GO:0070925 GO:0071214 GO:0071241 GO:0071248 GO:0071277 GO:0071470 GO:0071472 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072657 GO:0080050 GO:0080090 GO:0080134 GO:0090068 GO:0090303 GO:0090329 GO:0090392 GO:0090627 GO:0090628 GO:0097264 GO:0097435 GO:0097708 GO:0098588 GO:0098771 GO:0098772 GO:0098805 GO:0098827 GO:0099080 GO:0099081 GO:0099512 GO:0104004 GO:0140096 GO:1901564 GO:1902531 GO:1902679 GO:1902680 GO:1903034 GO:1903036 GO:1903169 GO:1903320 GO:1903321 GO:1903506 GO:1903507 GO:1903508 GO:1904062 GO:1904064 GO:1904427 GO:2000011 GO:2000014 GO:2000024 GO:2000026 GO:2000105 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calpain_III Peptidase_C2

Protein Analysis

2161

Amino Acids

239.31

Weight (kDa)

6.21

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C2 PF00648 1705 - 2004 3.2e-88 Calpain family cysteine protease
Calpain_III PF01067 2015 - 2150 6.2e-16 Calpain large subunit, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 5791, 6059
Acc16I TGCGCA 1 cut(s) 5646
Acc36I ACCTGC 5 cut(s) 1157, 2252, 5176, 5791, 6059
AccB1I GGYRCC 2 cut(s) 1856, 3257
AccB7I CCANNNNNTGG 3 cut(s) 144, 1236, 3254
AccI GTMKAC 5 cut(s) 2451, 2500, 5601, 5674, 6015
AccII CGCG 3 cut(s) 3945, 4153, 6143
AcoI YGGCCR 6 cut(s) 146, 2055, 4258, 4392, 5818, 6046
AcuI CTGAAG 7 cut(s) 1143, 1383, 3009, 3228, 4769, 4785, 4851
AdeI CACNNNGTG 1 cut(s) 6179
AflII CTTAAG 1 cut(s) 2317
AgeI ACCGGT 2 cut(s) 2532, 4598
AhlI ACTAGT 2 cut(s) 4496, 5492
AjnI CCWGG 8 cut(s) 110, 137, 148, 1173, 3984, 5003, 5966, 6415
AjuI GAANNNNNNNTTGG 6 cut(s) 4633, 4638, 4665, 4670, 5973, 6005
AleI CACNNNNGTG 1 cut(s) 6395
Alw21I GWGCWC 5 cut(s) 374, 1208, 1220, 3661, 3930
Alw26I GTCTC 6 cut(s) 201, 1825, 2706, 3790, 5688, 6083
Alw44I GTGCAC 1 cut(s) 1216
AlwNI CAGNNNCTG 3 cut(s) 812, 2267, 4254
Ama87I CYCGRG 7 cut(s) 1534, 2751, 3860, 4128, 6023, 6273, 6342
ApaLI GTGCAC 1 cut(s) 1216
ArsI GACNNNNNNTTYG 4 cut(s) 1376, 1408, 5129, 5161
AseI ATTAAT 1 cut(s) 1281
AsiGI ACCGGT 2 cut(s) 2532, 4598
Asp700I GAANNNNTTC 4 cut(s) 864, 3427, 5059, 5712
AspA2I CCTAGG 1 cut(s) 5021
AspLEI GCGC 8 cut(s) 995, 1149, 3945, 4153, 5647, 5697, 6033, 6145
AsuC2I CCSGG 1 cut(s) 5472
AsuII TTCGAA 1 cut(s) 858
AsuNHI GCTAGC 1 cut(s) 28
AvaI CYCGRG 7 cut(s) 1534, 2751, 3860, 4128, 6023, 6273, 6342
AvaII GGWCC 8 cut(s) 48, 164, 229, 632, 931, 2264, 2931, 3865
AvrII CCTAGG 1 cut(s) 5021
BaeGI GKGCMC 3 cut(s) 954, 1220, 1373
BalI TGGCCA 5 cut(s) 148, 2057, 4260, 5820, 6048
BamHI GGATCC 4 cut(s) 1615, 1654, 2217, 6370
BanI GGYRCC 2 cut(s) 1856, 3257
BanII GRGCYC 2 cut(s) 374, 3930
BarI GAAGNNNNNNTAC 2 cut(s) 3330, 3362
BauI CACGAG 1 cut(s) 1269
BbsI GAAGAC 1 cut(s) 2538
Bbv12I GWGCWC 5 cut(s) 374, 1208, 1220, 3661, 3930
BbvCI CCTCAGC 1 cut(s) 168
BceAI ACGGC 3 cut(s) 5309, 5965, 6264
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BciT130I CCWGG 8 cut(s) 112, 139, 150, 1175, 3986, 5005, 5968, 6417
BciVI GTATCC 2 cut(s) 204, 6255
BclI TGATCA 6 cut(s) 240, 1396, 3637, 4440, 5119, 5140
BcnI CCSGG 1 cut(s) 5472
BcoDI GTCTC 6 cut(s) 201, 1825, 2706, 3790, 5688, 6083
BcuI ACTAGT 2 cut(s) 4496, 5492
BfmI CTRYAG 7 cut(s) 121, 2268, 2550, 3586, 4080, 4671, 4890
BfoI RGCGCY 1 cut(s) 996
BfrI CTTAAG 1 cut(s) 2317
BfuAI ACCTGC 5 cut(s) 1157, 2252, 5176, 5791, 6059
BfuI GTATCC 2 cut(s) 204, 6255
BglII AGATCT 2 cut(s) 3389, 4900
BlnI CCTAGG 1 cut(s) 5021
BlpI GCTNAGC 2 cut(s) 2102, 4801
BmcAI AGTACT 1 cut(s) 237
Bme1390I CCNGG 9 cut(s) 112, 139, 150, 1175, 3986, 5005, 5472, 5968, 6417
Bme18I GGWCC 8 cut(s) 48, 164, 229, 632, 931, 2264, 2931, 3865
BmeT110I CYCGRG 7 cut(s) 1534, 2751, 3860, 4128, 6023, 6273, 6342
BmrFI CCNGG 9 cut(s) 112, 139, 150, 1175, 3986, 5005, 5472, 5968, 6417
BmrI ACTGGG 1 cut(s) 3116
BmtI GCTAGC 1 cut(s) 32
BmuI ACTGGG 1 cut(s) 3116
BoxI GACNNNNGTC 1 cut(s) 5680
BpiI GAAGAC 1 cut(s) 2538
BpmI CTGGAG 5 cut(s) 133, 4007, 4927, 5631, 5746
Bpu10I CCTNAGC 2 cut(s) 168, 3762
Bpu1102I GCTNAGC 2 cut(s) 2102, 4801
Bpu14I TTCGAA 1 cut(s) 858
BpuEI CTTGAG 5 cut(s) 358, 1829, 2155, 3311, 6175
BpuMI CCSGG 1 cut(s) 5472
Bsa29I ATCGAT 1 cut(s) 1299
BsaAI YACGTR 1 cut(s) 6157
BsaBI GATNNNNATC 5 cut(s) 1704, 3636, 4884, 4899, 6375
BsaI GGTCTC 1 cut(s) 5688
BsaWI WCCGGW 3 cut(s) 226, 2532, 4598
BsaXI ACNNNNNCTCC 6 cut(s) 2522, 2552, 4474, 4504, 5522, 5552
Bse118I RCCGGY 3 cut(s) 2532, 4394, 4598
Bse3DI GCAATG 7 cut(s) 368, 1026, 1322, 1511, 4146, 4607, 4850
Bse8I GATNNNNATC 5 cut(s) 1704, 3636, 4884, 4899, 6375
BseBI CCWGG 8 cut(s) 112, 139, 150, 1175, 3986, 5005, 5968, 6417
BseCI ATCGAT 1 cut(s) 1299
BseJI GATNNNNATC 5 cut(s) 1704, 3636, 4884, 4899, 6375
BseMI GCAATG 7 cut(s) 368, 1026, 1322, 1511, 4146, 4607, 4850
BseRI GAGGAG 5 cut(s) 3575, 3938, 3965, 5639, 5653
BseSI GKGCMC 3 cut(s) 954, 1220, 1373
BseYI CCCAGC 3 cut(s) 3059, 3213, 4377
BsgI GTGCAG 2 cut(s) 2280, 6087
Bsh1236I CGCG 3 cut(s) 3945, 4153, 6143
BshNI GGYRCC 2 cut(s) 1856, 3257
BshTI ACCGGT 2 cut(s) 2532, 4598
BshVI ATCGAT 1 cut(s) 1299
BsiHKAI GWGCWC 5 cut(s) 374, 1208, 1220, 3661, 3930
BsiHKCI CYCGRG 7 cut(s) 1534, 2751, 3860, 4128, 6023, 6273, 6342
BsiSI CCGG 6 cut(s) 227, 2337, 2533, 4395, 4599, 5471
BsiWI CGTACG 1 cut(s) 423
BslFI GGGAC 4 cut(s) 61, 2159, 3297, 6337
BsmAI GTCTC 6 cut(s) 201, 1825, 2706, 3790, 5688, 6083
BsmFI GGGAC 4 cut(s) 61, 2159, 3297, 6337
BsmI GAATGC 1 cut(s) 5061
Bso31I GGTCTC 1 cut(s) 5688
BsoBI CYCGRG 7 cut(s) 1534, 2751, 3860, 4128, 6023, 6273, 6342
Bsp119I TTCGAA 1 cut(s) 858
Bsp1286I GDGCHC 7 cut(s) 374, 954, 1208, 1220, 1373, 3661, 3930
Bsp1720I GCTNAGC 2 cut(s) 2102, 4801
Bsp19I CCATGG 3 cut(s) 160, 2831, 5846
BspDI ATCGAT 1 cut(s) 1299
BspFNI CGCG 3 cut(s) 3945, 4153, 6143
BspHI TCATGA 3 cut(s) 2868, 3531, 6229
BspMAI CTGCAG 5 cut(s) 2272, 2554, 3590, 4084, 4894
BspMI ACCTGC 5 cut(s) 1157, 2252, 5176, 5791, 6059
BspOI GCTAGC 1 cut(s) 32
BspQI GCTCTTC 2 cut(s) 1640, 4788
BspT104I TTCGAA 1 cut(s) 858
BspT107I GGYRCC 2 cut(s) 1856, 3257
BspTI CTTAAG 1 cut(s) 2317
BspTNI GGTCTC 1 cut(s) 5688
BsrDI GCAATG 7 cut(s) 368, 1026, 1322, 1511, 4146, 4607, 4850
BsrFI RCCGGY 3 cut(s) 2532, 4394, 4598
BssAI RCCGGY 3 cut(s) 2532, 4394, 4598
BssNAI GTATAC 1 cut(s) 2452
BssSI CACGAG 1 cut(s) 1269
Bst1107I GTATAC 1 cut(s) 2452
Bst2BI CACGAG 1 cut(s) 1269
Bst2UI CCWGG 8 cut(s) 112, 139, 150, 1175, 3986, 5005, 5968, 6417
Bst6I CTCTTC 8 cut(s) 1553, 1640, 2043, 3640, 3678, 3915, 3942, 4788
BstAFI CTTAAG 1 cut(s) 2317
BstAPI GCANNNNNTGC 1 cut(s) 2267
BstBAI YACGTR 1 cut(s) 6157
BstBI TTCGAA 1 cut(s) 858
BstDSI CCRYGG 3 cut(s) 160, 2831, 5846
BstEII GGTNACC 1 cut(s) 2228
BstENI CCTNNNNNAGG 2 cut(s) 1076, 3989
BstFNI CGCG 3 cut(s) 3945, 4153, 6143
BstH2I RGCGCY 1 cut(s) 996
BstHHI GCGC 8 cut(s) 995, 1149, 3945, 4153, 5647, 5697, 6033, 6145
BstMAI GTCTC 6 cut(s) 201, 1825, 2706, 3790, 5688, 6083
BstNI CCWGG 8 cut(s) 112, 139, 150, 1175, 3986, 5005, 5968, 6417
BstNSI RCATGY 7 cut(s) 36, 532, 645, 846, 2193, 4616, 5933
BstPAI GACNNNNGTC 1 cut(s) 5680
BstPI GGTNACC 1 cut(s) 2228
BstSCI CCNGG 9 cut(s) 110, 137, 148, 1173, 3984, 5003, 5470, 5966, 6415
BstSFI CTRYAG 7 cut(s) 121, 2268, 2550, 3586, 4080, 4671, 4890
BstSLI GKGCMC 3 cut(s) 954, 1220, 1373
BstUI CGCG 3 cut(s) 3945, 4153, 6143
BstV2I GAAGAC 1 cut(s) 2538
BstZ17I GTATAC 1 cut(s) 2452
Bsu15I ATCGAT 1 cut(s) 1299
BsuI GTATCC 2 cut(s) 204, 6255
BsuTUI ATCGAT 1 cut(s) 1299
BtgI CCRYGG 3 cut(s) 160, 2831, 5846
BtsI GCAGTG 6 cut(s) 312, 952, 2559, 4089, 4317, 4983
BveI ACCTGC 5 cut(s) 1157, 2252, 5176, 5791, 6059
CaiI CAGNNNCTG 3 cut(s) 812, 2267, 4254
CciI TCATGA 3 cut(s) 2868, 3531, 6229
CfoI GCGC 8 cut(s) 995, 1149, 3945, 4153, 5647, 5697, 6033, 6145
Cfr10I RCCGGY 3 cut(s) 2532, 4394, 4598
ClaI ATCGAT 1 cut(s) 1299
CseI GACGC 4 cut(s) 5, 1703, 2430, 4142
CspAI ACCGGT 2 cut(s) 2532, 4598
CspCI CAANNNNNGTGG 2 cut(s) 1896, 1931
DraIII CACNNNGTG 1 cut(s) 6179
EaeI YGGCCR 6 cut(s) 146, 2055, 4258, 4392, 5818, 6046
Eam1104I CTCTTC 8 cut(s) 1553, 1640, 2043, 3640, 3678, 3915, 3942, 4788
EarI CTCTTC 8 cut(s) 1553, 1640, 2043, 3640, 3678, 3915, 3942, 4788
EciI GGCGGA 1 cut(s) 3602
Ecl136II GAGCTC 2 cut(s) 372, 3928
Eco147I AGGCCT 1 cut(s) 5209
Eco24I GRGCYC 2 cut(s) 374, 3930
Eco31I GGTCTC 1 cut(s) 5688
Eco32I GATATC 1 cut(s) 1726
Eco47I GGWCC 8 cut(s) 48, 164, 229, 632, 931, 2264, 2931, 3865
Eco53kI GAGCTC 2 cut(s) 372, 3928
Eco57I CTGAAG 7 cut(s) 1143, 1383, 3009, 3228, 4769, 4785, 4851
Eco88I CYCGRG 7 cut(s) 1534, 2751, 3860, 4128, 6023, 6273, 6342
Eco91I GGTNACC 1 cut(s) 2228
EcoICRI GAGCTC 2 cut(s) 372, 3928
EcoNI CCTNNNNNAGG 2 cut(s) 1076, 3989
EcoO109I RGGNCCY 5 cut(s) 48, 108, 632, 2264, 2931
EcoO65I GGTNACC 1 cut(s) 2228
EcoRII CCWGG 8 cut(s) 110, 137, 148, 1173, 3984, 5003, 5966, 6415
EcoRV GATATC 1 cut(s) 1726
EcoT22I ATGCAT 1 cut(s) 4728
EcoT38I GRGCYC 2 cut(s) 374, 3930
FaqI GGGAC 4 cut(s) 61, 2159, 3297, 6337
FauI CCCGC 2 cut(s) 2081, 4080
FauNDI CATATG 2 cut(s) 245, 2437
FbaI TGATCA 6 cut(s) 240, 1396, 3637, 4440, 5119, 5140
FblI GTMKAC 5 cut(s) 2451, 2500, 5601, 5674, 6015
FriOI GRGCYC 2 cut(s) 374, 3930
FspI TGCGCA 1 cut(s) 5646
GlaI GCGC 8 cut(s) 994, 1148, 3944, 4152, 5646, 5696, 6032, 6144
GsaI CCCAGC 3 cut(s) 3063, 3217, 4381
GsuI CTGGAG 5 cut(s) 133, 4007, 4927, 5631, 5746
HaeII RGCGCY 1 cut(s) 996
HapII CCGG 6 cut(s) 227, 2337, 2533, 4395, 4599, 5471
HgaI GACGC 4 cut(s) 5, 1703, 2430, 4142
HhaI GCGC 8 cut(s) 995, 1149, 3945, 4153, 5647, 5697, 6033, 6145
Hin6I GCGC 8 cut(s) 993, 1147, 3943, 4151, 5645, 5695, 6031, 6143
HinP1I GCGC 8 cut(s) 993, 1147, 3943, 4151, 5645, 5695, 6031, 6143
HincII GTYRAC 5 cut(s) 1738, 5602, 5675, 6171, 6337
HindII GTYRAC 5 cut(s) 1738, 5602, 5675, 6171, 6337
HindIII AAGCTT 3 cut(s) 2422, 4020, 5825
HpaII CCGG 6 cut(s) 227, 2337, 2533, 4395, 4599, 5471
Hpy99I CGWCG 4 cut(s) 21, 4158, 4185, 6285
HpyCH4IV ACGT 5 cut(s) 784, 3790, 4969, 6156, 6167
HpySE526I ACGT 5 cut(s) 784, 3790, 4969, 6156, 6167
HspAI GCGC 8 cut(s) 993, 1147, 3943, 4151, 5645, 5695, 6031, 6143
KflI GGGWCCC 1 cut(s) 48
Ksp22I TGATCA 6 cut(s) 240, 1396, 3637, 4440, 5119, 5140
LguI GCTCTTC 2 cut(s) 1640, 4788
LmnI GCTCC 6 cut(s) 794, 1203, 3439, 3925, 5561, 6433
MaeII ACGT 5 cut(s) 784, 3790, 4969, 6156, 6167
MfeI CAATTG 5 cut(s) 1908, 1920, 2775, 5687, 6393
MhlI GDGCHC 7 cut(s) 374, 954, 1208, 1220, 1373, 3661, 3930
MlsI TGGCCA 5 cut(s) 148, 2057, 4260, 5820, 6048
MluNI TGGCCA 5 cut(s) 148, 2057, 4260, 5820, 6048
MlyI GAGTC 7 cut(s) 788, 1733, 3316, 3792, 5239, 5474, 5879
MmeI TCCRAC 6 cut(s) 107, 2095, 2666, 2971, 3451, 6139
Mox20I TGGCCA 5 cut(s) 148, 2057, 4260, 5820, 6048
Mph1103I ATGCAT 1 cut(s) 4728
MroXI GAANNNNTTC 4 cut(s) 864, 3427, 5059, 5712
MscI TGGCCA 5 cut(s) 148, 2057, 4260, 5820, 6048
MslI CAYNNNNRTG 4 cut(s) 1412, 3485, 6254, 6395
Msp20I TGGCCA 5 cut(s) 148, 2057, 4260, 5820, 6048
MspA1I CMGCKG 8 cut(s) 172, 452, 472, 809, 2810, 3458, 4079, 4805
MspCI CTTAAG 1 cut(s) 2317
MspI CCGG 6 cut(s) 227, 2337, 2533, 4395, 4599, 5471
MspR9I CCNGG 9 cut(s) 112, 139, 150, 1175, 3986, 5005, 5472, 5968, 6417
MunI CAATTG 5 cut(s) 1908, 1920, 2775, 5687, 6393
Mva1269I GAATGC 1 cut(s) 5061
MvaI CCWGG 8 cut(s) 112, 139, 150, 1175, 3986, 5005, 5968, 6417
MvnI CGCG 3 cut(s) 3945, 4153, 6143
NciI CCSGG 1 cut(s) 5472
NcoI CCATGG 3 cut(s) 160, 2831, 5846
NdeI CATATG 2 cut(s) 245, 2437
NheI GCTAGC 1 cut(s) 28
NmuCI GTSAC 6 cut(s) 780, 1529, 1712, 2491, 2770, 5466
NsbI TGCGCA 1 cut(s) 5646
NsiI ATGCAT 1 cut(s) 4728
NspI RCATGY 7 cut(s) 36, 532, 645, 846, 2193, 4616, 5933
NspV TTCGAA 1 cut(s) 858
OliI CACNNNNGTG 1 cut(s) 6395
PaeI GCATGC 3 cut(s) 36, 846, 2193
PaeR7I CTCGAG 3 cut(s) 4128, 6273, 6342
PagI TCATGA 3 cut(s) 2868, 3531, 6229
PaqCI CACCTGC 2 cut(s) 5791, 6059
PceI AGGCCT 1 cut(s) 5209
PciSI GCTCTTC 2 cut(s) 1640, 4788
PcsI WCGNNNNNNNCGW 1 cut(s) 6280
PctI GAATGC 1 cut(s) 5061
PdmI GAANNNNTTC 4 cut(s) 864, 3427, 5059, 5712
Pfl23II CGTACG 1 cut(s) 423
PflMI CCANNNNNTGG 3 cut(s) 144, 1236, 3254
PfoI TCCNGGA 1 cut(s) 3984
PinAI ACCGGT 2 cut(s) 2532, 4598
PleI GAGTC 7 cut(s) 787, 1733, 3316, 3792, 5238, 5473, 5879
PpsI GAGTC 7 cut(s) 787, 1733, 3316, 3792, 5238, 5473, 5879
Ppu21I YACGTR 1 cut(s) 6157
PpuMI RGGWCCY 4 cut(s) 48, 632, 2264, 2931
PshAI GACNNNNGTC 1 cut(s) 5680
PshBI ATTAAT 1 cut(s) 1281
Psp124BI GAGCTC 2 cut(s) 374, 3930
Psp5II RGGWCCY 4 cut(s) 48, 632, 2264, 2931
Psp6I CCWGG 8 cut(s) 110, 137, 148, 1173, 3984, 5003, 5966, 6415
PspEI GGTNACC 1 cut(s) 2228
PspFI CCCAGC 3 cut(s) 3059, 3213, 4377
PspGI CCWGG 8 cut(s) 110, 137, 148, 1173, 3984, 5003, 5966, 6415
PspLI CGTACG 1 cut(s) 423
PspPPI RGGWCCY 4 cut(s) 48, 632, 2264, 2931
PspXI VCTCGAGB 1 cut(s) 6273
PstI CTGCAG 5 cut(s) 2272, 2554, 3590, 4084, 4894
PstNI CAGNNNCTG 3 cut(s) 812, 2267, 4254
PvuII CAGCTG 6 cut(s) 172, 452, 809, 2810, 4079, 4805
RseI CAYNNNNRTG 4 cut(s) 1412, 3485, 6254, 6395
SacI GAGCTC 2 cut(s) 374, 3930
SalI GTCGAC 2 cut(s) 5600, 5673
SapI GCTCTTC 2 cut(s) 1640, 4788
SbfI CCTGCAGG 1 cut(s) 2272
ScaI AGTACT 1 cut(s) 237
SchI GAGTC 7 cut(s) 788, 1733, 3316, 3792, 5239, 5474, 5879
ScrFI CCNGG 9 cut(s) 112, 139, 150, 1175, 3986, 5005, 5472, 5968, 6417
SdaI CCTGCAGG 1 cut(s) 2272
SduI GDGCHC 7 cut(s) 374, 954, 1208, 1220, 1373, 3661, 3930
SfcI CTRYAG 7 cut(s) 121, 2268, 2550, 3586, 4080, 4671, 4890
Sfr274I CTCGAG 3 cut(s) 4128, 6273, 6342
SfuI TTCGAA 1 cut(s) 858
SinI GGWCC 8 cut(s) 48, 164, 229, 632, 931, 2264, 2931, 3865
SlaI CTCGAG 3 cut(s) 4128, 6273, 6342
SmiMI CAYNNNNRTG 4 cut(s) 1412, 3485, 6254, 6395
SmlI CTYRAG 9 cut(s) 373, 1808, 2134, 2317, 3326, 4128, 6190, 6273, 6342
SmoI CTYRAG 9 cut(s) 373, 1808, 2134, 2317, 3326, 4128, 6190, 6273, 6342
SpeI ACTAGT 2 cut(s) 4496, 5492
SphI GCATGC 3 cut(s) 36, 846, 2193
Sse8387I CCTGCAGG 1 cut(s) 2272
SseBI AGGCCT 1 cut(s) 5209
SspI AATATT 4 cut(s) 270, 2328, 3672, 4386
SstI GAGCTC 2 cut(s) 374, 3930
StuI AGGCCT 1 cut(s) 5209
StyD4I CCNGG 9 cut(s) 110, 137, 148, 1173, 3984, 5003, 5470, 5966, 6415
TaiI ACGT 5 cut(s) 787, 3793, 4972, 6159, 6170
TatI WGTACW 8 cut(s) 235, 747, 1460, 2321, 2394, 2875, 3346, 3775
TauI GCSGC 2 cut(s) 3461, 6143
TseFI GTSAC 6 cut(s) 780, 1529, 1712, 2491, 2770, 5466
Tsp45I GTSAC 6 cut(s) 780, 1529, 1712, 2491, 2770, 5466
TspGWI ACGGA 5 cut(s) 49, 2210, 2437, 4294, 5354
Van91I CCANNNNNTGG 3 cut(s) 144, 1236, 3254
Vha464I CTTAAG 1 cut(s) 2317
VneI GTGCAC 1 cut(s) 1216
VpaK11BI GGWCC 8 cut(s) 48, 164, 229, 632, 931, 2264, 2931, 3865
VspI ATTAAT 1 cut(s) 1281
XagI CCTNNNNNAGG 2 cut(s) 1076, 3989
XbaI TCTAGA 4 cut(s) 1900, 2624, 4222, 4862
XceI RCATGY 7 cut(s) 36, 532, 645, 846, 2193, 4616, 5933
XcmI CCANNNNNNNNNTGG 2 cut(s) 1869, 2285
XhoI CTCGAG 3 cut(s) 4128, 6273, 6342
XmaJI CCTAGG 1 cut(s) 5021
XmiI GTMKAC 5 cut(s) 2451, 2500, 5601, 5674, 6015
XmnI GAANNNNTTC 4 cut(s) 864, 3427, 5059, 5712
ZrmI AGTACT 1 cut(s) 237
Zsp2I ATGCAT 1 cut(s) 4728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.