pycom11g00320

Belongs to the peptidase C2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0007783
Physical Location & Seq
Forward (+)
260992 .. 266289
5298 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g00320.11

Sequence Viewer

Length: 2745 bp
ATGGCTTCTGCGATTGCTTTGATGGTCACTGGTCTACTGCCGATTGTTTCATGGTTTGCAACATACCGGTTCTCCCTGTCTTCTGCAGTGTGTGTTGGGATATTCACAGTTGTCCTTGTGGCATTTTGTGGTGCATCCTATATGGAGGTTGTGAAATCTAGAGATGACCAAGTTCCAACAGAGGGTGATTTCCTTGCTGCTTTACTTCCTTTAATTTGCAGTCCTGCATTGTTGTCTCTTTGCTCTGGGTTACATAAATGGAAGGATGATGATTGGAAACTCTCTCGGGGTGTTTATATCTTTGTCACAATTGGGCTGCTGCTTCTGCTTGGGGCAATATCAGCTGTTATAGTTGTAGTTAAACCATGGACGGTTGGGGTGGCGTTTCTTTTAGTTCTTCTCATGATTGTACTAGCTATTGGTGCTATCCATCATTGGGCTTCAAACAATTTCTATTTGACAAGGACCCAGACATTCTTTGTTTGTTTCCTTGCATTTTTGTTGGCATTGGCAGCATTTCTTGTTGGATGGTTTGAAGATAAAGCATTTGTGGGTGCTTCAGTTGGTTATTTTCTATTCTTATTTCTTCTTGCTGGGAGAGCATTGACTGTTCTTCTTTCACCTCCCATTGTTGTTTATTCTCCAAGAGTTCTCCCTGTCTATGTTTATGATGCTCATGCAGATTGTGGAAAGAATGTCAGTGTGGCATTTCTAGTGCTTTATGGGATTGCATTGGCAACTGAAGGCTGGGGTGTTGTTGCCAGTTTGAAAATTTATCCACCATTTGCTGGTGCCTCTGTGTCAGCAATAACTCTTGTTGTAGCCTTTGGGTTTGCTTTCTCTCGTCCCTGCTTGACTCTCAAGATGATGGAAGATGCAGTACACTTTCTTAGTAAGGAAACCGTTGTTCAAGCTATTGCTAGATCTGCTACCAAGACCAGAAATGCTTTGTCTGGAACTTATTCTGCTCCACAAAGGTCTGCCAGTTCAGCGGCTCTCTTGGTTGGAGATCCCACCATTATGCGTGATAGGGCAGGAAATTTTGTGCTTCCCAGAGCAGATGTCATGAAATTTAGAGATCGTTTAAGAAATGAGGAGTTAGTTGCAGGCTCATTCTTTTGCAGAAAGAGATATGGAAGAACTTTCCGCCATGAACCAACTAATGATGTTGATCATAGAAGAGAGATGTGTGCTCATGCACGAATATTGGCTTTGGAAGAGGCGATTGATACTGAATGGGTGTATATGTGGGATAAATTTGGTGGGTATCTGCTTCTTTTGCTTGGTTTGACTGCTAAGGCAGAGCGAGTACAGGATGAGGTACGTTTGAGACTCTTTCTTGATAGCATAGGGTTTGCTGACCTAAGTGCCAAGAAAATAAAAAAGTGGATGCCCGAGGACCGCAGGCGGTTTGAGATTATTCAAGAGAGTTATATAAGAGAAAAAGAGATGGAAGAGGAGCTCTTAATGCAAAGGCGTGAAGAGGAGGGGAAAGGCAAAGAGAGAAGAAAGGCTCTCCTGGAGAAGGAAGAACGCAAGTGGAAGGAGATAGAAGCTTCTCTGATTTCCTCCATTCCTAATGCTGGCAATAGGGAAGCAGCAGCTATGGCAGCTGCAGTGCGGGCAGTAGGGGGTGATTCTGTTCTTGATGATTCTTTTGCTCGAGAGAGAGTTTCAAGCATTGCGCGTCGCATACGAACAGCTCAGTTGGCTCGTCGAGCAGTTCAGACTGGAATTTCTGGGGCTGTATGTGTTCTAGATGATGAGCCTATAACAAGTGGCAGACACTGTGGCCAGATTGACCCCACCATTTGTCAAAGTCAAAAGATTAGCTTCTCCGTTGCAGTGATGATTCAACCTGTATCTGGGCCTGTTTGCTTATTTGGCACTGAGTTTCAAAAGAAAGTTTGCTGGGAAATATTGGTGGCCGGTTCTGAGCAAGGTATTGAAGCTGGACAAGTTGGGCTTAGATTGATCACAAAAGGTGATAGACAAACTACTGTTGCAAAGGAGTGGAGTATTAGTGCAACTAGTATAGCAGATGGAAGGTGGCATTTGGTAACAATGACGATTGATGCTGATTTAGGTGAGGCAACTTGCTATCTGGATGGTGGTTTTGATGGCTACCAGACCAGTTTACCATTGCATGTGGGTAATACTGTCTGGGAACAAGGAACAGAAGTTTGGGTTGGTGTTAGACCACCTACAGATGTGGATGCGTTTGGGAGGTCAGACAGTGAAGGGGCTGAGTCTAAGATGCATATTATGGATGTGTTCCTATGGGGAAGGTGCTTAACTGAAGATGATGTTGCTGCTCTTTATTCAGCTATTGGCTCAGCTGATTCTGATATGATAGATTTTCCTGAAGATAATTGGCAATGGGCAGATTCACCCTCTAGAGTTGATGAATGGGATAGTGATCCTGCAGATGTAGATCTGTATGATAGGGATGACGTTGATTGGGATGGGCAATATTCTAGTGGGAGGAAACGAAGGGCAGAACGTGATGCAGTGCTGGTTGATGTGGATTCATTTGCCAGGAGGTTTCGGAAACCTAGGATGGAAACACAAGAAGAAATCAACCAGCGAATGCTTTCAGTTGAATTGGCAGTCAAGGAAGCCCTCTCTGCAAGGGGAGAAATACATTTTACTGATCAGGAGTTTCCGCCAAATGATCAGTCATTATTTGTCGGATCGATCCAGAAAATCCCCCATCAAAGTTGCAGGTTGTTTCTGAGTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001558 GO:0001708 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0004198 GO:0005198 GO:0005488 GO:0005509 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0005829 GO:0005886 GO:0005887 GO:0006275 GO:0006355 GO:0006508 GO:0006807 GO:0006873 GO:0006874 GO:0006875 GO:0006950 GO:0006970 GO:0006996 GO:0007010 GO:0007204 GO:0007275 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0008307 GO:0009628 GO:0009651 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009934 GO:0009966 GO:0009987 GO:0010008 GO:0010035 GO:0010038 GO:0010154 GO:0010468 GO:0010522 GO:0010524 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010646 GO:0010927 GO:0010941 GO:0010959 GO:0012505 GO:0014717 GO:0014718 GO:0014823 GO:0014850 GO:0016020 GO:0016021 GO:0016043 GO:0016787 GO:0019219 GO:0019222 GO:0019538 GO:0019725 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0030003 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030036 GO:0030154 GO:0030162 GO:0030234 GO:0030239 GO:0030315 GO:0031032 GO:0031090 GO:0031224 GO:0031226 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031402 GO:0031410 GO:0031420 GO:0031432 GO:0031674 GO:0031982 GO:0031984 GO:0032101 GO:0032268 GO:0032269 GO:0032270 GO:0032501 GO:0032502 GO:0032875 GO:0032877 GO:0032879 GO:0032947 GO:0032989 GO:0032991 GO:0033036 GO:0033233 GO:0033234 GO:0033554 GO:0034613 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0040008 GO:0042127 GO:0042175 GO:0042221 GO:0042383 GO:0042592 GO:0042692 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043269 GO:0043270 GO:0043292 GO:0043415 GO:0043416 GO:0043933 GO:0044085 GO:0044093 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044449 GO:0044459 GO:0044464 GO:0045165 GO:0045214 GO:0045740 GO:0045787 GO:0045862 GO:0045892 GO:0045893 GO:0045927 GO:0045934 GO:0045935 GO:0046872 GO:0048316 GO:0048468 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048583 GO:0048584 GO:0048608 GO:0048638 GO:0048639 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050801 GO:0050865 GO:0050867 GO:0050896 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051146 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051239 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051279 GO:0051281 GO:0051282 GO:0051480 GO:0051592 GO:0051641 GO:0051716 GO:0051726 GO:0051924 GO:0051928 GO:0055001 GO:0055002 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055103 GO:0060255 GO:0060341 GO:0060548 GO:0061041 GO:0061061 GO:0061458 GO:0065003 GO:0065007 GO:0065008 GO:0065009 GO:0070011 GO:0070727 GO:0070887 GO:0070925 GO:0071214 GO:0071241 GO:0071248 GO:0071277 GO:0071470 GO:0071472 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072657 GO:0080050 GO:0080090 GO:0080134 GO:0090068 GO:0090303 GO:0090329 GO:0090392 GO:0090627 GO:0090628 GO:0097264 GO:0097435 GO:0097708 GO:0098588 GO:0098771 GO:0098772 GO:0098805 GO:0098827 GO:0099080 GO:0099081 GO:0099512 GO:0104004 GO:0140096 GO:1901564 GO:1902531 GO:1902679 GO:1902680 GO:1903034 GO:1903036 GO:1903169 GO:1903320 GO:1903321 GO:1903506 GO:1903507 GO:1903508 GO:1904062 GO:1904064 GO:1904427 GO:2000011 GO:2000014 GO:2000024 GO:2000026 GO:2000105 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calpain_III Peptidase_C2

Protein Analysis

915

Amino Acids

101.52

Weight (kDa)

5.56

Isoelectric Point (pI)

37.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2715
AccB1I GGYRCC 1 cut(s) 791
AccB7I CCANNNNNTGG 1 cut(s) 788
AccI GTMKAC 1 cut(s) 34
AccII CGCG 1 cut(s) 1687
AciI CCGC 6 cut(s) 992, 1147, 1402, 1408, 1621, 2666
AclWI GGATC 4 cut(s) 1004, 2414, 2692, 2701
AcoI YGGCCR 2 cut(s) 1792, 1926
AcsI RAATTY 5 cut(s) 771, 1039, 1070, 1256, 1734
AcuI CTGAAG 4 cut(s) 543, 762, 2319, 2385
AfaI GTAC 4 cut(s) 411, 882, 1311, 1323
AfiI CCNNNNNNNGG 6 cut(s) 182, 436, 788, 1525, 1583, 1865
AgeI ACCGGT 1 cut(s) 66
AhlI ACTAGT 1 cut(s) 2030
AjnI CCWGG 2 cut(s) 1518, 2537
AjuI GAANNNNNNNTTGG 4 cut(s) 2167, 2172, 2199, 2204
Alw21I GWGCWC 2 cut(s) 1195, 1464
Alw26I GTCTC 2 cut(s) 240, 1324
AlwI GGATC 4 cut(s) 1004, 2414, 2692, 2701
AlwNI CAGNNNCTG 1 cut(s) 1788
Ama87I CYCGRG 3 cut(s) 285, 1394, 1662
AoxI GGCC 3 cut(s) 1792, 1868, 1926
ApeKI GCWGC 9 cut(s) 197, 316, 319, 512, 1598, 1601, 1610, 1613, 2312
ApoI RAATTY 5 cut(s) 771, 1039, 1070, 1256, 1734
ArsI GACNNNNNNTTYG 2 cut(s) 2663, 2695
AsiGI ACCGGT 1 cut(s) 66
Asp700I GAANNNNTTC 2 cut(s) 961, 2593
AspA2I CCTAGG 1 cut(s) 2555
AspLEI GCGC 1 cut(s) 1687
AspS9I GGNCC 3 cut(s) 465, 1399, 1868
AsuHPI GGTGA 6 cut(s) 197, 612, 1646, 1997, 2099, 2382
AvaI CYCGRG 3 cut(s) 285, 1394, 1662
AvaII GGWCC 2 cut(s) 465, 1399
AvrII CCTAGG 1 cut(s) 2555
BalI TGGCCA 1 cut(s) 1794
BanI GGYRCC 1 cut(s) 791
BanII GRGCYC 1 cut(s) 1464
BarI GAAGNNNNNNTAC 2 cut(s) 864, 896
BbsI GAAGAC 1 cut(s) 72
Bbv12I GWGCWC 2 cut(s) 1195, 1464
BbvI GCAGC 9 cut(s) 184, 303, 306, 524, 1600, 1610, 1613, 1622, 2299
BciT130I CCWGG 2 cut(s) 1520, 2539
BclI TGATCA 4 cut(s) 1171, 1974, 2653, 2674
BcoDI GTCTC 2 cut(s) 240, 1324
BcuI ACTAGT 1 cut(s) 2030
BfaI CTAG 9 cut(s) 159, 413, 713, 921, 1757, 2031, 2397, 2478, 2556
BfmI CTRYAG 4 cut(s) 84, 1614, 2205, 2424
BfuAI ACCTGC 1 cut(s) 2715
BglII AGATCT 2 cut(s) 923, 2434
BlnI CCTAGG 1 cut(s) 2555
BlpI GCTNAGC 1 cut(s) 2335
Bme1390I CCNGG 2 cut(s) 1520, 2539
Bme18I GGWCC 2 cut(s) 465, 1399
BmeT110I CYCGRG 3 cut(s) 285, 1394, 1662
BmgT120I GGNCC 3 cut(s) 465, 1399, 1868
BmiI GGNNCC 2 cut(s) 467, 793
BmrFI CCNGG 2 cut(s) 1520, 2539
BmsI GCATC 8 cut(s) 143, 661, 865, 1380, 2065, 2206, 2247, 2497
BpiI GAAGAC 1 cut(s) 72
BpmI CTGGAG 1 cut(s) 1541
Bpu10I CCTNAGC 1 cut(s) 1296
Bpu1102I GCTNAGC 1 cut(s) 2335
BpuEI CTTGAG 1 cut(s) 845
Bsa29I ATCGAT 1 cut(s) 2696
BsaBI GATNNNNATC 3 cut(s) 1170, 2418, 2433
BsaJI CCNNGG 3 cut(s) 365, 1395, 2555
BsaWI WCCGGW 1 cut(s) 66
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 2008, 2038
Bsc4I CCNNNNNNNGG 6 cut(s) 182, 436, 788, 1525, 1583, 1865
Bse118I RCCGGY 2 cut(s) 66, 1928
Bse1I ACTGG 5 cut(s) 34, 762, 984, 1735, 2133
Bse3DI GCAATG 3 cut(s) 1680, 2141, 2384
Bse8I GATNNNNATC 3 cut(s) 1170, 2418, 2433
BseBI CCWGG 2 cut(s) 1520, 2539
BseCI ATCGAT 1 cut(s) 2696
BseDI CCNNGG 3 cut(s) 365, 1395, 2555
BseJI GATNNNNATC 3 cut(s) 1170, 2418, 2433
BseLI CCNNNNNNNGG 6 cut(s) 182, 436, 788, 1525, 1583, 1865
BseMI GCAATG 3 cut(s) 1680, 2141, 2384
BseMII CTCAG 6 cut(s) 1718, 1881, 1926, 2238, 2349, 2726
BseNI ACTGG 5 cut(s) 34, 762, 984, 1735, 2133
BseRI GAGGAG 3 cut(s) 1109, 1472, 1499
BseXI GCAGC 9 cut(s) 184, 303, 306, 524, 1600, 1610, 1613, 1622, 2299
BseYI CCCAGC 3 cut(s) 593, 747, 1911
Bsh1236I CGCG 1 cut(s) 1687
BshFI GGCC 3 cut(s) 1794, 1870, 1928
BshNI GGYRCC 1 cut(s) 791
BshTI ACCGGT 1 cut(s) 66
BshVI ATCGAT 1 cut(s) 2696
BsiHKAI GWGCWC 2 cut(s) 1195, 1464
BsiHKCI CYCGRG 3 cut(s) 285, 1394, 1662
BsiSI CCGG 2 cut(s) 67, 1929
BslFI GGGAC 1 cut(s) 831
BslI CCNNNNNNNGG 6 cut(s) 182, 436, 788, 1525, 1583, 1865
BsmAI GTCTC 2 cut(s) 240, 1324
BsmFI GGGAC 1 cut(s) 831
BsmI GAATGC 1 cut(s) 2595
BsnI GGCC 3 cut(s) 1794, 1870, 1928
BsoBI CYCGRG 3 cut(s) 285, 1394, 1662
Bsp1286I GDGCHC 2 cut(s) 1195, 1464
Bsp1720I GCTNAGC 1 cut(s) 2335
Bsp19I CCATGG 1 cut(s) 365
BspACI CCGC 6 cut(s) 992, 1147, 1402, 1408, 1621, 2666
BspANI GGCC 3 cut(s) 1794, 1870, 1928
BspCNI CTCAG 6 cut(s) 1717, 1882, 1927, 2239, 2348, 2727
BspDI ATCGAT 1 cut(s) 2696
BspFNI CGCG 1 cut(s) 1687
BspHI TCATGA 2 cut(s) 402, 1065
BspLI GGNNCC 2 cut(s) 467, 793
BspMAI CTGCAG 3 cut(s) 88, 1618, 2428
BspMI ACCTGC 1 cut(s) 2715
BspPI GGATC 4 cut(s) 1004, 2414, 2692, 2701
BspT107I GGYRCC 1 cut(s) 791
BsrDI GCAATG 3 cut(s) 1680, 2141, 2384
BsrFI RCCGGY 2 cut(s) 66, 1928
BsrI ACTGG 5 cut(s) 34, 762, 984, 1735, 2133
BssAI RCCGGY 2 cut(s) 66, 1928
BssECI CCNNGG 3 cut(s) 365, 1395, 2555
BssT1I CCWWGG 2 cut(s) 365, 2555
Bst2UI CCWGG 2 cut(s) 1520, 2539
Bst4CI ACNGT 8 cut(s) 109, 373, 610, 904, 1790, 2002, 2161, 2237
Bst6I CTCTTC 4 cut(s) 1174, 1212, 1449, 1476
BstC8I GCNNGC 4 cut(s) 1108, 1406, 1585, 1623
BstDSI CCRYGG 1 cut(s) 365
BstENI CCTNNNNNAGG 1 cut(s) 1523
BstFNI CGCG 1 cut(s) 1687
BstHHI GCGC 1 cut(s) 1687
BstMAI GTCTC 2 cut(s) 240, 1324
BstNI CCWGG 2 cut(s) 1520, 2539
BstNSI RCATGY 1 cut(s) 2150
BstSCI CCNGG 2 cut(s) 1518, 2537
BstSFI CTRYAG 4 cut(s) 84, 1614, 2205, 2424
BstUI CGCG 1 cut(s) 1687
BstV1I GCAGC 9 cut(s) 184, 303, 306, 524, 1600, 1610, 1613, 1622, 2299
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 3 cut(s) 923, 1009, 2434
BstYI RGATCY 3 cut(s) 923, 1009, 2434
Bsu15I ATCGAT 1 cut(s) 2696
BsuRI GGCC 3 cut(s) 1794, 1870, 1928
BsuTUI ATCGAT 1 cut(s) 2696
BtgI CCRYGG 1 cut(s) 365
BtsI GCAGTG 4 cut(s) 93, 1623, 1851, 2517
BtsIMutI CAGTG 9 cut(s) 27, 93, 706, 1623, 1786, 1851, 1887, 2242, 2517
BveI ACCTGC 1 cut(s) 2715
Cac8I GCNNGC 4 cut(s) 1108, 1406, 1585, 1623
CaiI CAGNNNCTG 1 cut(s) 1788
CciI TCATGA 2 cut(s) 402, 1065
CfoI GCGC 1 cut(s) 1687
Cfr10I RCCGGY 2 cut(s) 66, 1928
Cfr13I GGNCC 3 cut(s) 465, 1399, 1868
ClaI ATCGAT 1 cut(s) 2696
CseI GACGC 1 cut(s) 1676
Csp6I GTAC 4 cut(s) 410, 881, 1310, 1322
CspAI ACCGGT 1 cut(s) 66
CviAII CATG 8 cut(s) 51, 366, 403, 677, 1066, 1151, 1196, 2147
CviQI GTAC 4 cut(s) 410, 881, 1310, 1322
EaeI YGGCCR 2 cut(s) 1792, 1926
Eam1104I CTCTTC 4 cut(s) 1174, 1212, 1449, 1476
EarI CTCTTC 4 cut(s) 1174, 1212, 1449, 1476
EciI GGCGGA 2 cut(s) 1136, 2655
Ecl136II GAGCTC 1 cut(s) 1462
Eco130I CCWWGG 2 cut(s) 365, 2555
Eco24I GRGCYC 1 cut(s) 1464
Eco47I GGWCC 2 cut(s) 465, 1399
Eco53kI GAGCTC 1 cut(s) 1462
Eco57I CTGAAG 4 cut(s) 543, 762, 2319, 2385
Eco88I CYCGRG 3 cut(s) 285, 1394, 1662
EcoICRI GAGCTC 1 cut(s) 1462
EcoNI CCTNNNNNAGG 1 cut(s) 1523
EcoO109I RGGNCCY 1 cut(s) 465
EcoRII CCWGG 2 cut(s) 1518, 2537
EcoT14I CCWWGG 2 cut(s) 365, 2555
EcoT22I ATGCAT 1 cut(s) 2262
EcoT38I GRGCYC 1 cut(s) 1464
ErhI CCWWGG 2 cut(s) 365, 2555
FaeI CATG 8 cut(s) 54, 369, 406, 680, 1069, 1154, 1199, 2150
FalI AAGNNNNNCTT 4 cut(s) 1817, 1849, 1950, 1982
FaqI GGGAC 1 cut(s) 831
FatI CATG 8 cut(s) 50, 365, 402, 676, 1065, 1150, 1195, 2146
FauI CCCGC 1 cut(s) 1614
FbaI TGATCA 4 cut(s) 1171, 1974, 2653, 2674
FblI GTMKAC 1 cut(s) 34
FriOI GRGCYC 1 cut(s) 1464
FspBI CTAG 9 cut(s) 159, 413, 713, 921, 1757, 2031, 2397, 2478, 2556
GlaI GCGC 1 cut(s) 1686
GsaI CCCAGC 3 cut(s) 597, 751, 1915
GsuI CTGGAG 1 cut(s) 1541
HaeIII GGCC 3 cut(s) 1794, 1870, 1928
HapII CCGG 2 cut(s) 67, 1929
HgaI GACGC 1 cut(s) 1676
HhaI GCGC 1 cut(s) 1687
Hin1II CATG 8 cut(s) 54, 369, 406, 680, 1069, 1154, 1199, 2150
Hin6I GCGC 1 cut(s) 1685
HinP1I GCGC 1 cut(s) 1685
HindIII AAGCTT 1 cut(s) 1554
HinfI GANTC 9 cut(s) 856, 1332, 1637, 1652, 1852, 2249, 2342, 2387, 2528
HpaII CCGG 2 cut(s) 67, 1929
HphI GGTGA 6 cut(s) 197, 612, 1646, 1997, 2099, 2382
Hpy166II GTNNAC 3 cut(s) 35, 883, 2138
Hpy188I TCNGA 8 cut(s) 1563, 1728, 1936, 2233, 2347, 2550, 2693, 2736
Hpy8I GTNNAC 3 cut(s) 35, 883, 2138
Hpy99I CGWCG 2 cut(s) 1692, 1719
HpyAV CCTTC 8 cut(s) 256, 737, 1519, 1537, 2040, 2234, 2280, 2487
HpyCH4III ACNGT 8 cut(s) 109, 373, 610, 904, 1790, 2002, 2161, 2237
HpyCH4IV ACGT 3 cut(s) 1324, 2454, 2503
HpySE526I ACGT 3 cut(s) 1324, 2454, 2503
Hsp92II CATG 8 cut(s) 54, 369, 406, 680, 1069, 1154, 1199, 2150
HspAI GCGC 1 cut(s) 1685
Ksp22I TGATCA 4 cut(s) 1171, 1974, 2653, 2674
LmnI GCTCC 2 cut(s) 973, 1459
Lsp1109I GCAGC 9 cut(s) 184, 303, 306, 524, 1600, 1610, 1613, 1622, 2299
LweI GCATC 8 cut(s) 143, 661, 865, 1380, 2065, 2206, 2247, 2497
MaeI CTAG 9 cut(s) 159, 413, 713, 921, 1757, 2031, 2397, 2478, 2556
MaeII ACGT 3 cut(s) 1324, 2454, 2503
MaeIII GTNAC 4 cut(s) 25, 249, 304, 2059
MfeI CAATTG 1 cut(s) 309
MflI RGATCY 3 cut(s) 923, 1009, 2434
MhlI GDGCHC 2 cut(s) 1195, 1464
MlsI TGGCCA 1 cut(s) 1794
MluNI TGGCCA 1 cut(s) 1794
MlyI GAGTC 3 cut(s) 850, 1326, 2258
MmeI TCCRAC 4 cut(s) 200, 505, 985, 2671
Mox20I TGGCCA 1 cut(s) 1794
Mph1103I ATGCAT 1 cut(s) 2262
MroXI GAANNNNTTC 2 cut(s) 961, 2593
MscI TGGCCA 1 cut(s) 1794
MseI TTAA 5 cut(s) 212, 360, 1085, 1466, 2294
MslI CAYNNNNRTG 1 cut(s) 1019
Msp20I TGGCCA 1 cut(s) 1794
MspA1I CMGCKG 4 cut(s) 344, 992, 1613, 2339
MspI CCGG 2 cut(s) 67, 1929
MspR9I CCNGG 2 cut(s) 1520, 2539
MunI CAATTG 1 cut(s) 309
Mva1269I GAATGC 1 cut(s) 2595
MvaI CCWGG 2 cut(s) 1520, 2539
MvnI CGCG 1 cut(s) 1687
NcoI CCATGG 1 cut(s) 365
NlaIII CATG 8 cut(s) 54, 369, 406, 680, 1069, 1154, 1199, 2150
NlaIV GGNNCC 2 cut(s) 467, 793
NmuCI GTSAC 2 cut(s) 25, 304
NsiI ATGCAT 1 cut(s) 2262
NspI RCATGY 1 cut(s) 2150
PaeR7I CTCGAG 1 cut(s) 1662
PagI TCATGA 2 cut(s) 402, 1065
PctI GAATGC 1 cut(s) 2595
PdmI GAANNNNTTC 2 cut(s) 961, 2593
PfeI GAWTC 6 cut(s) 1637, 1652, 1852, 2342, 2387, 2528
PflMI CCANNNNNTGG 1 cut(s) 788
PfoI TCCNGGA 1 cut(s) 1518
PinAI ACCGGT 1 cut(s) 66
PleI GAGTC 3 cut(s) 850, 1326, 2257
PpsI GAGTC 3 cut(s) 850, 1326, 2257
PpuMI RGGWCCY 1 cut(s) 465
Psp124BI GAGCTC 1 cut(s) 1464
Psp5II RGGWCCY 1 cut(s) 465
Psp6I CCWGG 2 cut(s) 1518, 2537
PspFI CCCAGC 3 cut(s) 593, 747, 1911
PspGI CCWGG 2 cut(s) 1518, 2537
PspN4I GGNNCC 2 cut(s) 467, 793
PspPI GGNCC 3 cut(s) 465, 1399, 1868
PspPPI RGGWCCY 1 cut(s) 465
PstI CTGCAG 3 cut(s) 88, 1618, 2428
PstNI CAGNNNCTG 1 cut(s) 1788
PsuI RGATCY 3 cut(s) 923, 1009, 2434
PvuII CAGCTG 3 cut(s) 344, 1613, 2339
RsaI GTAC 4 cut(s) 411, 882, 1311, 1323
RsaNI GTAC 4 cut(s) 410, 881, 1310, 1322
RseI CAYNNNNRTG 1 cut(s) 1019
SacI GAGCTC 1 cut(s) 1464
SaqAI TTAA 5 cut(s) 212, 360, 1085, 1466, 2294
Sau96I GGNCC 3 cut(s) 465, 1399, 1868
SchI GAGTC 3 cut(s) 850, 1326, 2258
ScrFI CCNGG 2 cut(s) 1520, 2539
SduI GDGCHC 2 cut(s) 1195, 1464
SfaNI GCATC 8 cut(s) 143, 661, 865, 1380, 2065, 2206, 2247, 2497
SfcI CTRYAG 4 cut(s) 84, 1614, 2205, 2424
Sfr274I CTCGAG 1 cut(s) 1662
SinI GGWCC 2 cut(s) 465, 1399
SlaI CTCGAG 1 cut(s) 1662
SmiMI CAYNNNNRTG 1 cut(s) 1019
SmlI CTYRAG 2 cut(s) 860, 1662
SmoI CTYRAG 2 cut(s) 860, 1662
SpeI ACTAGT 1 cut(s) 2030
SsiI CCGC 6 cut(s) 992, 1147, 1402, 1408, 1621, 2666
SspI AATATT 3 cut(s) 1206, 1920, 2474
SspMI CTAG 9 cut(s) 159, 413, 713, 921, 1757, 2031, 2397, 2478, 2556
SstI GAGCTC 1 cut(s) 1464
StyD4I CCNGG 2 cut(s) 1518, 2537
StyI CCWWGG 2 cut(s) 365, 2555
TaaI ACNGT 8 cut(s) 109, 373, 610, 904, 1790, 2002, 2161, 2237
TaiI ACGT 3 cut(s) 1327, 2457, 2506
TaqI TCGA 3 cut(s) 1663, 1717, 2696
TatI WGTACW 3 cut(s) 409, 880, 1309
TauI GCSGC 1 cut(s) 995
TfiI GAWTC 6 cut(s) 1637, 1652, 1852, 2342, 2387, 2528
Tru1I TTAA 5 cut(s) 212, 360, 1085, 1466, 2294
Tru9I TTAA 5 cut(s) 212, 360, 1085, 1466, 2294
TscAI CASTG 9 cut(s) 34, 93, 706, 1623, 1793, 1851, 1894, 2242, 2517
TseFI GTSAC 2 cut(s) 25, 304
TseI GCWGC 9 cut(s) 197, 316, 319, 512, 1598, 1601, 1610, 1613, 2312
Tsp45I GTSAC 2 cut(s) 25, 304
TspDTI ATGAA 5 cut(s) 39, 1082, 1167, 2421, 2520
TspGWI ACGGA 1 cut(s) 1828
TspRI CASTG 9 cut(s) 34, 93, 706, 1623, 1793, 1851, 1894, 2242, 2517
Van91I CCANNNNNTGG 1 cut(s) 788
VpaK11BI GGWCC 2 cut(s) 465, 1399
XagI CCTNNNNNAGG 1 cut(s) 1523
XapI RAATTY 5 cut(s) 771, 1039, 1070, 1256, 1734
XbaI TCTAGA 3 cut(s) 158, 1756, 2396
XceI RCATGY 1 cut(s) 2150
XhoI CTCGAG 1 cut(s) 1662
XmaJI CCTAGG 1 cut(s) 2555
XmiI GTMKAC 1 cut(s) 34
XmnI GAANNNNTTC 2 cut(s) 961, 2593
XspI CTAG 9 cut(s) 159, 413, 713, 921, 1757, 2031, 2397, 2478, 2556
Zsp2I ATGCAT 1 cut(s) 2262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.