pycom03g00370

Belongs to the peptidase C2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007783
Physical Location & Seq
Forward (+)
305860 .. 314880
9021 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g00370.22

Sequence Viewer

Length: 4464 bp
ATGGCTTTCCTTCGATCTCTTCAAGCAGAGGAAATGGCCATGACCTCAAAACCCCGCAAGTATGGCTTTGTTGCTTGGCTGCTCAGTACTTCTGTTGGATTGCTGCTTTCCTTCTTAAGCAAATCATCAGTTCTTCTTGGATTGTCCTTGACTGTCCCACTCATGGTGGCATGCCTCTCTGTTGCAATTCCTATATGGATACGTAATGGCTACCAGTTTAGGGTTCCTCAATTACAATGTGCAGGTCCTGTAGGGAACCATCAAATTCGAGGAACAAAAGAGGGTGTTATTCTTGTCTTAAGTACAATATTATTTGCTGGATCTGTGTTAGCTCTTGGTGCGATAGTGTCTGCAAAGCCCTTAGATGACTTAAAATACAAAGGCTGGACTGGTGAACAGAAAAGCTTTACCTCTCCTTATGCGTCATCTGTGTACATTGGCTGGGCTATGGCTTCTGCCATTGCTTTGGTGGTCACTGGTCTACTGCCAATTGTTTCATGGTTTGCAACATACCGGTTCTCCCTGTCTTCTGCAGTGTGTGTTGGGATATTCACAGTTGTCCTTGTGGCATTTTGTGGTGCATCCTATATGGAGGTGGTGAAATCTAGAGATGACCAAGTTCCAACAGAGGGTGATTTCCTTGCTGCTTTACTTCCTTTAATTTGCAGTCCTGCATTGCTGTCTCTTTGCTCTGGGTTACATAAATGGAAGGATGATGATTGGAAACTCTCTCGGGGTGTTTATATCTTTGTCACCATTGGGCTTCTTCTTCTGCTTGGAGCAATATCAGCTGTTATAGTTGTAGTTAGACCATGGACAATTGGGGTAGCATTTCTTTTAGTTCTTCTCATGATTGTACTAGCTATTGGTGCTATCCATCATTGGGCGTCAAACAATTTCTATTTAACAAGGACCCAGACATTCTTTGTTTGTTTCCTTGCTTTTTTGTTGGCATTGGCGGCATTTCTTGTTGGATGGTTTGAAGATAAGGCATTTGTGGGTGCTTCTGTTGGTTATTTTCTGTTCTTATTTCTTCTAGCTGGGAGAGCATTGACTGTTCTTCTTTCACCTCCTATTGTTGTTTATTCTCCGAGAGTTCTCCCTGTCTATGTTTATGATGCTCATGCAGATTGTGGAAAGAATGTCAGTGCGACATTTCTATTGCTTTATGGGATTGCATTGGCAACTGAAGGCTGGGGTGTTGTTGCGAGTCTGAAAATTTATCCACCATTTGCCGGTGCCTCTGTGTCAGCACTCACTCTTGTTGTAGCCTTTGGGTTTGCTTTCTCTCGTCCCTGCTTGACCCTCAAGATGATGGAAGATGCAGTACACTTTCTTAGTAAGGAAACTGTTGTTCAAGCTATTGCTAGATCTGCTACCAAGACCAGAAATGCTTTGTCTGGAACTTATTCTGCTCCACAGAGGTCTGCCAGTTCAGCGGCTCTCTTGGTTGGAGATCCCACCATTACGCGTGATAGGGCAGGAAATTTTGTGCTTCCCAGAGCAGATGTAGTGAAATTGAGAGATCGTTTAAGAAATGAGGAGTTAGTTGCAGGCTCATTCTTCTGCAGAAAGAGATATGGAAGAACTTTCCGCCATGAACCAACTAATGATGTTAATCATAGAAGAGAGATGTGTGCTCATGCACGAATATTGGCTTTGGAAGAGGCGATTGATACTGAATGGGTGTATATGTGGGATAAATTTGGTGGGTATCTGCTTCTTTTGCTTGGTTTGACTGCTAAGGCTGAGCGAGTACAGGATGAGGTACGTTTGAGACTCTTTCTCGATAGCATAGGGTTTGCTGACCTAAGTGCCAAGAAAATAAAAAAGTGGATGCCAGAGGACCGCAGGCAGTTTGAGATTATTCAAGAGAGTTATATAAGAGAAAAAGAGATGGAAGAGGAGCTCTTAGTGCAAAGGCGCGAAGAGGAGGGCAAAGGCAAAGTGAGAAGAAAGGCTCTTCTGGAGAAGGAAGAACGCAAGTGGAAGGAGATAGAAGCTTCTCTGATTTCCTACATTCCTAATGCTGGCAACAGGGAAGCAGCAGCTATGGCAGCTGCAGTGCGTGCAGTAGGAGGTGATTCTGTTCTTGATGATTCTTTTGCTCGAGAGAGAGTTTCAAGCATTGCGCGTCGCATACGCACAGCTCAGTTGGCTCGTCGAGCAGTCCAGACCGGAATTTCTGGGGCTGTATGCGTTCTTGATGATGAGCCAACCACAAGTGGGAGACACTGTGGCCACATTGATCCCACCATTTGTCAAAGTCAAAAGATTAGCTTCTCCGTTACAGTGATGATTCAACCTGTATCTGGGCCTGTTTGCTTATTTGGCACTGAGTTTCAAAAGAAAGATTGCTGGGAAATATTGGTGGCAGGTTCTGAGCAAGGTATTGAAGCTGGACAAGTTGGGCTTAGATTGATAACGAAAGGTGATAGACAAACTACTGTTGCAAAGGAGTGGAGTATTAGTGCAACTAGTATAGCAGATGGAAGGTGGCATTTGGTAACAATGACAATTGATGCTGATTTAGGTGAGGCAACTTGCTATCTGGACGGTGGTTTTGATGGCTACCAGACAGGTTTACCATTGCAAGTGGGTAATACTATTTGGGAAGAAGGAACCGAAGTTTGGGTTGGTGTTAGACCACCGACAGATGTGGATGCGTTTGGGAGGTCAGACAGTGAAGGGGCTGAGTCTAAGATGCATATTATGGATGTGTTCCTATGGGGAAGGTGCTTAACTGAAGATGATGTTGCTGCTCTTCATTCAGCTATTGGTTCAGCTGATTCCGATATGATAGATTTTCCTGAAGATAATTGGCAGTGGGCAGATTCACCCTCTAGAGTTGATGAATGGGATAGTGATCCTGCGGATGTAGATCTGTATGATAGGGATGACGTTGATTGGGACGGGCAATATTCTAGTGGGAGGAAACGAAGGTCAGAACGTGATGCAGTGCTGGTTGATGTGGATTCAATTGCCAGGAGGTTTCGGAAACCTAGGATGGAAACACAAGACGAAATCAACCAGCGAATGCTTTCAGTTGAATTGGCAGTCAAGGAAGCCCTCTCTGCAAGGGGAGAAATACATTTTACCGATCAGGAGTTTCCTCCAAATGATCAGTCATTATTTGTAGATCCGGAAAATCCCCCATCAAAGTTGCAGGTTGTTTCTGAATGGGTGAGGCCTGCTGATATTGTAAAAGAGAGTCGGCTGGATGCTCGTCCATGCTTATTTTCTGGAACCGTCAATCCTTCTGATGTTTGTCAGGGCCGACTTGGTGATTGCTGGTTTTTGAGTGCCGTTGCTGTTCTGACGGAGGTTTCACGAATATCGGAAGTGATCATTACCCCAGAGTATAATGAGGAAGGAATCTACACTGTTCGCTTCTGTATTCAGGGTGAGTGGGTTCCTGTTGTTGTTGATGATTGGATTCCATGTGAGTCACCGGGGAAACCAGCGTTTGCTACTAGTAGGAAAGGTAACGAGCTATGGGTTTCTTTAGTGGAGAAGGCGTATGCTAAATTGCATGGCTCCTATGAAGCATTAGAAGGTGGGCTTGTCCAAGATGCTCTTGTCGACCTTACTGGAGGTGCTGGTGAGGAGATTGACATGAGGAGTGCTCAGGCCCAAATTGATCTTGCAAGTGGTCGACTATGGTCTCAATTGCTGCGTTTCAAACAAGAAGGCTTTCTTCTTGGTGCTGGCAGTCCATCAGGTTCAGATGTACATGTTTCCTCCAGTGGCATTGTGCAAGGTCATGCCTACTCGTTATTGCAGGTGAGAGAGGTGGATGGCCACAAGCTTATTCAGATTCGGAATCCATGGGCTAATGAAGTTGAGTGGAATGGCCCTTGGTCGGACTCATCACCTCTTTGGACTGATAGGATGAAGCATAAGTTGAAGCATGTTCAGCAGTCAAAAGACGGCATATTCTGGATGTCCTGGCAAGACTTCCAAATTCATTTTCGATCAATATATGTTTGTCGTATCTACCCCCCTGAGATGCGCTATTCTGTTCATGGTCAATGGCGAGGTTATAGTGCTGGTGGCTGCCAAGATTATGAGACATGGCATCAAAATCCACAGTTCCGATTGAGGGCCACTGGGCCAGATGCTTCGCTTCCAATTCATGTATTCATTACGTTGACACAGGGTGTGAGCTTCTCAAGAACAGTTGCTGGTTTCAGAAATTATCAATCCAGTCATGATTCAATGATGTTCTACATTGGGATGAGGATACTCAAAACTCGAGGCCGTCGCGCTGCTTACAATATATACCTACATGAATCAGTTGATGGGACAGATTACGTCAATTCTCGAGAAATATCTTGTGAAATTGTTTTGGATCGAGATCCAAAAGGTTACACAATAGTGCCTACAACTATACATCCAGGGGAAGAAGCTCCATTTGTTCTTTCTGTTTTCACAAAAGCATCAATTACCTTGGAAGCCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001558 GO:0001708 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0004198 GO:0005198 GO:0005488 GO:0005509 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0005829 GO:0005886 GO:0005887 GO:0006275 GO:0006355 GO:0006508 GO:0006807 GO:0006873 GO:0006874 GO:0006875 GO:0006950 GO:0006970 GO:0006996 GO:0007010 GO:0007204 GO:0007275 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0008307 GO:0009628 GO:0009651 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009934 GO:0009966 GO:0009987 GO:0010008 GO:0010035 GO:0010038 GO:0010154 GO:0010468 GO:0010522 GO:0010524 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010646 GO:0010927 GO:0010941 GO:0010959 GO:0012505 GO:0014717 GO:0014718 GO:0014823 GO:0014850 GO:0016020 GO:0016021 GO:0016043 GO:0016787 GO:0019219 GO:0019222 GO:0019538 GO:0019725 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0030003 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030036 GO:0030154 GO:0030162 GO:0030234 GO:0030239 GO:0030315 GO:0031032 GO:0031090 GO:0031224 GO:0031226 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031402 GO:0031410 GO:0031420 GO:0031432 GO:0031674 GO:0031982 GO:0031984 GO:0032101 GO:0032268 GO:0032269 GO:0032270 GO:0032501 GO:0032502 GO:0032875 GO:0032877 GO:0032879 GO:0032947 GO:0032989 GO:0032991 GO:0033036 GO:0033233 GO:0033234 GO:0033554 GO:0034613 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0040008 GO:0042127 GO:0042175 GO:0042221 GO:0042383 GO:0042592 GO:0042692 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043269 GO:0043270 GO:0043292 GO:0043415 GO:0043416 GO:0043933 GO:0044085 GO:0044093 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044449 GO:0044459 GO:0044464 GO:0045165 GO:0045214 GO:0045740 GO:0045787 GO:0045862 GO:0045892 GO:0045893 GO:0045927 GO:0045934 GO:0045935 GO:0046872 GO:0048316 GO:0048468 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048583 GO:0048584 GO:0048608 GO:0048638 GO:0048639 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050801 GO:0050865 GO:0050867 GO:0050896 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051146 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051239 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051279 GO:0051281 GO:0051282 GO:0051480 GO:0051592 GO:0051641 GO:0051716 GO:0051726 GO:0051924 GO:0051928 GO:0055001 GO:0055002 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055103 GO:0060255 GO:0060341 GO:0060548 GO:0061041 GO:0061061 GO:0061458 GO:0065003 GO:0065007 GO:0065008 GO:0065009 GO:0070011 GO:0070727 GO:0070887 GO:0070925 GO:0071214 GO:0071241 GO:0071248 GO:0071277 GO:0071470 GO:0071472 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072657 GO:0080050 GO:0080090 GO:0080134 GO:0090068 GO:0090303 GO:0090329 GO:0090392 GO:0090627 GO:0090628 GO:0097264 GO:0097435 GO:0097708 GO:0098588 GO:0098771 GO:0098772 GO:0098805 GO:0098827 GO:0099080 GO:0099081 GO:0099512 GO:0104004 GO:0140096 GO:1901564 GO:1902531 GO:1902679 GO:1902680 GO:1903034 GO:1903036 GO:1903169 GO:1903320 GO:1903321 GO:1903506 GO:1903507 GO:1903508 GO:1904062 GO:1904064 GO:1904427 GO:2000011 GO:2000014 GO:2000024 GO:2000026 GO:2000105 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calpain_III Peptidase_C2

Protein Analysis

1488

Amino Acids

165.19

Weight (kDa)

5.8

Isoelectric Point (pI)

38.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3772
Acc36I ACCTGC 4 cut(s) 233, 2366, 3157, 3772
AccB1I GGYRCC 1 cut(s) 1238
AccI GTMKAC 3 cut(s) 481, 3582, 3655
AccII CGCG 4 cut(s) 1471, 1926, 2134, 4266
AccIII TCCGGA 1 cut(s) 3142
AciI CCGC 6 cut(s) 55, 959, 1439, 1594, 1849, 2873
AclWI GGATC 7 cut(s) 328, 1451, 2243, 2861, 3134, 4352, 4359
AcoI YGGCCR 3 cut(s) 36, 2239, 3799
AcsI RAATTY 6 cut(s) 264, 1218, 1486, 1703, 2181, 3963
AcuI CTGAAG 3 cut(s) 1209, 2766, 2832
AcyI GRCGYC 1 cut(s) 887
AdeI CACNNNGTG 1 cut(s) 4160
AfaI GTAC 8 cut(s) 88, 304, 434, 858, 1329, 1758, 1770, 3732
AflII CTTAAG 2 cut(s) 115, 298
AflIII ACRYGT 2 cut(s) 1469, 3733
AgeI ACCGGT 1 cut(s) 513
AhlI ACTAGT 2 cut(s) 2477, 3473
AjnI CCWGG 3 cut(s) 2984, 3947, 4396
AjuI GAANNNNNNNTTGG 6 cut(s) 2614, 2619, 2646, 2651, 3954, 3986
AleI CACNNNNGTG 1 cut(s) 4376
AloI GAACNNNNNNTCC 2 cut(s) 3238, 3270
Alw21I GWGCWC 3 cut(s) 1642, 1911, 3628
Alw26I GTCTC 5 cut(s) 687, 1771, 2224, 3669, 4064
AlwI GGATC 7 cut(s) 328, 1451, 2243, 2861, 3134, 4352, 4359
AlwNI CAGNNNCTG 3 cut(s) 248, 2381, 4184
Ama87I CYCGRG 4 cut(s) 732, 2109, 4254, 4323
Aor13HI TCCGGA 1 cut(s) 3142
ApoI RAATTY 6 cut(s) 264, 1218, 1486, 1703, 2181, 3963
ArsI GACNNNNNNTTYG 4 cut(s) 2546, 2578, 3110, 3142
AsiGI ACCGGT 1 cut(s) 513
Asp700I GAANNNNTTC 3 cut(s) 1408, 3040, 3693
AspA2I CCTAGG 1 cut(s) 3002
AspLEI GCGC 4 cut(s) 1926, 2134, 4014, 4268
AspS9I GGNCC 9 cut(s) 245, 912, 1846, 2315, 3274, 3631, 3854, 4104, 4112
AsuC2I CCSGG 1 cut(s) 3453
AvaI CYCGRG 4 cut(s) 732, 2109, 4254, 4323
AvaII GGWCC 3 cut(s) 245, 912, 1846
AvrII CCTAGG 1 cut(s) 3002
BalI TGGCCA 3 cut(s) 38, 2241, 3801
BanI GGYRCC 1 cut(s) 1238
BanII GRGCYC 1 cut(s) 1911
BarI GAAGNNNNNNTAC 2 cut(s) 1311, 1343
BbsI GAAGAC 1 cut(s) 519
Bbv12I GWGCWC 3 cut(s) 1642, 1911, 3628
BceAI ACGGC 3 cut(s) 3290, 3946, 4245
BciT130I CCWGG 3 cut(s) 2986, 3949, 4398
BciVI GTATCC 2 cut(s) 192, 4236
BclI TGATCA 2 cut(s) 3121, 3345
BcnI CCSGG 1 cut(s) 3453
BcoDI GTCTC 5 cut(s) 687, 1771, 2224, 3669, 4064
BcuI ACTAGT 2 cut(s) 2477, 3473
BfaI CTAG 9 cut(s) 606, 860, 1037, 1368, 2478, 2844, 2925, 3003, 3474
BfmI CTRYAG 4 cut(s) 249, 531, 1567, 2061
BfrI CTTAAG 2 cut(s) 115, 298
BfuAI ACCTGC 4 cut(s) 233, 2366, 3157, 3772
BfuI GTATCC 2 cut(s) 192, 4236
BglII AGATCT 2 cut(s) 1370, 2881
BlnI CCTAGG 1 cut(s) 3002
BlpI GCTNAGC 1 cut(s) 1749
BmcAI AGTACT 1 cut(s) 88
Bme1390I CCNGG 4 cut(s) 2986, 3453, 3949, 4398
Bme18I GGWCC 3 cut(s) 245, 912, 1846
BmeT110I CYCGRG 4 cut(s) 732, 2109, 4254, 4323
BmgT120I GGNCC 9 cut(s) 245, 912, 1846, 2315, 3274, 3631, 3854, 4104, 4112
BmiI GGNNCC 8 cut(s) 225, 257, 914, 1240, 2623, 3247, 3414, 3538
BmrFI CCNGG 4 cut(s) 2986, 3453, 3949, 4398
BmrI ACTGGG 1 cut(s) 4119
BmuI ACTGGG 1 cut(s) 4119
BoxI GACNNNNGTC 1 cut(s) 3661
BpiI GAAGAC 1 cut(s) 519
BplI GAGNNNNNCTC 2 cut(s) 3610, 3642
BpmI CTGGAG 3 cut(s) 1988, 3612, 3727
Bpu10I CCTNAGC 2 cut(s) 1743, 3627
Bpu1102I GCTNAGC 1 cut(s) 1749
BpuEI CTTGAG 2 cut(s) 1292, 4156
BpuMI CCSGG 1 cut(s) 3453
BsaAI YACGTR 1 cut(s) 203
BsaBI GATNNNNATC 4 cut(s) 1617, 2865, 2880, 4356
BsaHI GRCGYC 1 cut(s) 887
BsaI GGTCTC 1 cut(s) 3669
BsaJI CCNNGG 7 cut(s) 812, 3002, 3452, 3827, 3857, 4397, 4449
BsaWI WCCGGW 3 cut(s) 513, 2177, 3142
BsaXI ACNNNNNCTCC 8 cut(s) 503, 533, 2221, 2251, 2455, 2485, 3503, 3533
Bse118I RCCGGY 2 cut(s) 513, 1235
Bse1I ACTGG 8 cut(s) 214, 394, 481, 1431, 3595, 3744, 4114, 4206
Bse3DI GCAATG 4 cut(s) 459, 674, 2127, 2588
Bse8I GATNNNNATC 4 cut(s) 1617, 2865, 2880, 4356
BseAI TCCGGA 1 cut(s) 3142
BseBI CCWGG 3 cut(s) 2986, 3949, 4398
BseDI CCNNGG 7 cut(s) 812, 3002, 3452, 3827, 3857, 4397, 4449
BseJI GATNNNNATC 4 cut(s) 1617, 2865, 2880, 4356
BseMI GCAATG 4 cut(s) 459, 674, 2127, 2588
BseMII CTCAG 8 cut(s) 97, 1740, 2165, 2328, 2373, 2685, 3641, 3996
BseNI ACTGG 8 cut(s) 214, 394, 481, 1431, 3595, 3744, 4114, 4206
BseRI GAGGAG 5 cut(s) 1556, 1919, 1946, 3620, 3634
BseYI CCCAGC 4 cut(s) 441, 1040, 1194, 2358
BsgI GTGCAG 2 cut(s) 261, 2091
Bsh1236I CGCG 4 cut(s) 1471, 1926, 2134, 4266
BshNI GGYRCC 1 cut(s) 1238
BshTI ACCGGT 1 cut(s) 513
BsiHKAI GWGCWC 3 cut(s) 1642, 1911, 3628
BsiHKCI CYCGRG 4 cut(s) 732, 2109, 4254, 4323
BsiSI CCGG 5 cut(s) 514, 1236, 2178, 3143, 3452
BslFI GGGAC 4 cut(s) 140, 1278, 2924, 4318
BsmAI GTCTC 5 cut(s) 687, 1771, 2224, 3669, 4064
BsmFI GGGAC 4 cut(s) 140, 1278, 2924, 4318
BsmI GAATGC 1 cut(s) 3042
Bso31I GGTCTC 1 cut(s) 3669
BsoBI CYCGRG 4 cut(s) 732, 2109, 4254, 4323
Bsp1286I GDGCHC 3 cut(s) 1642, 1911, 3628
Bsp13I TCCGGA 1 cut(s) 3142
Bsp1407I TGTACA 2 cut(s) 432, 3730
Bsp1720I GCTNAGC 1 cut(s) 1749
Bsp19I CCATGG 2 cut(s) 812, 3827
BspACI CCGC 6 cut(s) 55, 959, 1439, 1594, 1849, 2873
BspCNI CTCAG 8 cut(s) 96, 1741, 2164, 2329, 2374, 2686, 3640, 3997
BspEI TCCGGA 1 cut(s) 3142
BspFNI CGCG 4 cut(s) 1471, 1926, 2134, 4266
BspHI TCATGA 2 cut(s) 849, 4210
BspLI GGNNCC 8 cut(s) 225, 257, 914, 1240, 2623, 3247, 3414, 3538
BspMAI CTGCAG 3 cut(s) 535, 1571, 2065
BspMI ACCTGC 4 cut(s) 233, 2366, 3157, 3772
BspPI GGATC 7 cut(s) 328, 1451, 2243, 2861, 3134, 4352, 4359
BspQI GCTCTTC 2 cut(s) 1968, 2769
BspT107I GGYRCC 1 cut(s) 1238
BspTI CTTAAG 2 cut(s) 115, 298
BspTNI GGTCTC 1 cut(s) 3669
BsrDI GCAATG 4 cut(s) 459, 674, 2127, 2588
BsrFI RCCGGY 2 cut(s) 513, 1235
BsrGI TGTACA 2 cut(s) 432, 3730
BsrI ACTGG 8 cut(s) 214, 394, 481, 1431, 3595, 3744, 4114, 4206
BssAI RCCGGY 2 cut(s) 513, 1235
BssECI CCNNGG 7 cut(s) 812, 3002, 3452, 3827, 3857, 4397, 4449
BssNI GRCGYC 1 cut(s) 887
BssT1I CCWWGG 5 cut(s) 812, 3002, 3827, 3857, 4449
Bst2UI CCWGG 3 cut(s) 2986, 3949, 4398
Bst6I CTCTTC 7 cut(s) 24, 1621, 1659, 1896, 1923, 1968, 2769
BstACI GRCGYC 1 cut(s) 887
BstAFI CTTAAG 2 cut(s) 115, 298
BstAPI GCANNNNNTGC 1 cut(s) 2069
BstAUI TGTACA 2 cut(s) 432, 3730
BstBAI YACGTR 1 cut(s) 203
BstC8I GCNNGC 7 cut(s) 172, 1555, 1853, 2032, 2070, 3192, 3709
BstDSI CCRYGG 2 cut(s) 812, 3827
BstFNI CGCG 4 cut(s) 1471, 1926, 2134, 4266
BstHHI GCGC 4 cut(s) 1926, 2134, 4014, 4268
BstMAI GTCTC 5 cut(s) 687, 1771, 2224, 3669, 4064
BstNI CCWGG 3 cut(s) 2986, 3949, 4398
BstNSI RCATGY 3 cut(s) 174, 3737, 3914
BstPAI GACNNNNGTC 1 cut(s) 3661
BstSCI CCNGG 4 cut(s) 2984, 3451, 3947, 4396
BstSFI CTRYAG 4 cut(s) 249, 531, 1567, 2061
BstSNI TACGTA 1 cut(s) 203
BstUI CGCG 4 cut(s) 1471, 1926, 2134, 4266
BstV2I GAAGAC 1 cut(s) 519
BstX2I RGATCY 6 cut(s) 320, 1370, 1456, 2881, 3139, 4357
BstXI CCANNNNNNTGG 1 cut(s) 466
BstYI RGATCY 6 cut(s) 320, 1370, 1456, 2881, 3139, 4357
BsuI GTATCC 2 cut(s) 192, 4236
BtgI CCRYGG 2 cut(s) 812, 3827
BtsI GCAGTG 4 cut(s) 540, 2070, 2831, 2964
BveI ACCTGC 4 cut(s) 233, 2366, 3157, 3772
Cac8I GCNNGC 7 cut(s) 172, 1555, 1853, 2032, 2070, 3192, 3709
CaiI CAGNNNCTG 3 cut(s) 248, 2381, 4184
CciI TCATGA 2 cut(s) 849, 4210
CfoI GCGC 4 cut(s) 1926, 2134, 4014, 4268
Cfr10I RCCGGY 2 cut(s) 513, 1235
Cfr13I GGNCC 9 cut(s) 245, 912, 1846, 2315, 3274, 3631, 3854, 4104, 4112
CseI GACGC 3 cut(s) 411, 876, 2123
Csp6I GTAC 8 cut(s) 87, 303, 433, 857, 1328, 1757, 1769, 3731
CspAI ACCGGT 1 cut(s) 513
CviQI GTAC 8 cut(s) 87, 303, 433, 857, 1328, 1757, 1769, 3731
DraIII CACNNNGTG 1 cut(s) 4160
EaeI YGGCCR 3 cut(s) 36, 2239, 3799
Eam1104I CTCTTC 7 cut(s) 24, 1621, 1659, 1896, 1923, 1968, 2769
EarI CTCTTC 7 cut(s) 24, 1621, 1659, 1896, 1923, 1968, 2769
EciI GGCGGA 1 cut(s) 1583
Ecl136II GAGCTC 1 cut(s) 1909
Eco105I TACGTA 1 cut(s) 203
Eco130I CCWWGG 5 cut(s) 812, 3002, 3827, 3857, 4449
Eco147I AGGCCT 1 cut(s) 3190
Eco24I GRGCYC 1 cut(s) 1911
Eco31I GGTCTC 1 cut(s) 3669
Eco47I GGWCC 3 cut(s) 245, 912, 1846
Eco53kI GAGCTC 1 cut(s) 1909
Eco57I CTGAAG 3 cut(s) 1209, 2766, 2832
Eco88I CYCGRG 4 cut(s) 732, 2109, 4254, 4323
EcoICRI GAGCTC 1 cut(s) 1909
EcoO109I RGGNCCY 2 cut(s) 245, 912
EcoRII CCWGG 3 cut(s) 2984, 3947, 4396
EcoT14I CCWWGG 5 cut(s) 812, 3002, 3827, 3857, 4449
EcoT22I ATGCAT 1 cut(s) 2709
EcoT38I GRGCYC 1 cut(s) 1911
ErhI CCWWGG 5 cut(s) 812, 3002, 3827, 3857, 4449
FaqI GGGAC 4 cut(s) 140, 1278, 2924, 4318
FauI CCCGC 1 cut(s) 62
FbaI TGATCA 2 cut(s) 3121, 3345
FblI GTMKAC 3 cut(s) 481, 3582, 3655
FriOI GRGCYC 1 cut(s) 1911
FspBI CTAG 9 cut(s) 606, 860, 1037, 1368, 2478, 2844, 2925, 3003, 3474
GlaI GCGC 4 cut(s) 1925, 2133, 4013, 4267
GsaI CCCAGC 4 cut(s) 445, 1044, 1198, 2362
GsuI CTGGAG 3 cut(s) 1988, 3612, 3727
HapII CCGG 5 cut(s) 514, 1236, 2178, 3143, 3452
HgaI GACGC 3 cut(s) 411, 876, 2123
HhaI GCGC 4 cut(s) 1926, 2134, 4014, 4268
Hin1I GRCGYC 1 cut(s) 887
Hin6I GCGC 4 cut(s) 1924, 2132, 4012, 4266
HinP1I GCGC 4 cut(s) 1924, 2132, 4012, 4266
HincII GTYRAC 3 cut(s) 3583, 3656, 4152
HindII GTYRAC 3 cut(s) 3583, 3656, 4152
HindIII AAGCTT 3 cut(s) 403, 2001, 3806
HpaII CCGG 5 cut(s) 514, 1236, 2178, 3143, 3452
Hpy166II GTNNAC 8 cut(s) 395, 433, 482, 1330, 2585, 3583, 3656, 4152
Hpy8I GTNNAC 8 cut(s) 395, 433, 482, 1330, 2585, 3583, 3656, 4152
Hpy99I CGWCG 3 cut(s) 2139, 2166, 4266
HpyCH4IV ACGT 6 cut(s) 202, 1771, 2901, 2950, 4148, 4314
HpySE526I ACGT 6 cut(s) 202, 1771, 2901, 2950, 4148, 4314
Hsp92I GRCGYC 1 cut(s) 887
HspAI GCGC 4 cut(s) 1924, 2132, 4012, 4266
Kpn2I TCCGGA 1 cut(s) 3142
Ksp22I TGATCA 2 cut(s) 3121, 3345
LguI GCTCTTC 2 cut(s) 1968, 2769
LmnI GCTCC 5 cut(s) 779, 1420, 1906, 3542, 4414
MaeI CTAG 9 cut(s) 606, 860, 1037, 1368, 2478, 2844, 2925, 3003, 3474
MaeII ACGT 6 cut(s) 202, 1771, 2901, 2950, 4148, 4314
MaeIII GTNAC 8 cut(s) 472, 696, 751, 2287, 2506, 3447, 3485, 4367
MfeI CAATTG 5 cut(s) 489, 819, 2517, 2979, 3668
MflI RGATCY 6 cut(s) 320, 1370, 1456, 2881, 3139, 4357
MhlI GDGCHC 3 cut(s) 1642, 1911, 3628
MlsI TGGCCA 3 cut(s) 38, 2241, 3801
MluI ACGCGT 1 cut(s) 1469
MluNI TGGCCA 3 cut(s) 38, 2241, 3801
MlyI GAGTC 6 cut(s) 1219, 1773, 2705, 3220, 3455, 3860
MmeI TCCRAC 5 cut(s) 76, 647, 952, 1432, 3843
Mox20I TGGCCA 3 cut(s) 38, 2241, 3801
Mph1103I ATGCAT 1 cut(s) 2709
MroI TCCGGA 1 cut(s) 3142
MroXI GAANNNNTTC 3 cut(s) 1408, 3040, 3693
MscI TGGCCA 3 cut(s) 38, 2241, 3801
MseI TTAA 8 cut(s) 116, 299, 371, 659, 905, 1532, 1617, 2741
MslI CAYNNNNRTG 2 cut(s) 4235, 4376
Msp20I TGGCCA 3 cut(s) 38, 2241, 3801
MspA1I CMGCKG 4 cut(s) 791, 1439, 2060, 2786
MspCI CTTAAG 2 cut(s) 115, 298
MspI CCGG 5 cut(s) 514, 1236, 2178, 3143, 3452
MspR9I CCNGG 4 cut(s) 2986, 3453, 3949, 4398
MunI CAATTG 5 cut(s) 489, 819, 2517, 2979, 3668
Mva1269I GAATGC 1 cut(s) 3042
MvaI CCWGG 3 cut(s) 2986, 3949, 4398
MvnI CGCG 4 cut(s) 1471, 1926, 2134, 4266
NciI CCSGG 1 cut(s) 3453
NcoI CCATGG 2 cut(s) 812, 3827
NlaIV GGNNCC 8 cut(s) 225, 257, 914, 1240, 2623, 3247, 3414, 3538
NmuCI GTSAC 3 cut(s) 472, 751, 3447
NsiI ATGCAT 1 cut(s) 2709
NspI RCATGY 3 cut(s) 174, 3737, 3914
OliI CACNNNNGTG 1 cut(s) 4376
PaeI GCATGC 1 cut(s) 174
PaeR7I CTCGAG 3 cut(s) 2109, 4254, 4323
PagI TCATGA 2 cut(s) 849, 4210
PaqCI CACCTGC 1 cut(s) 3772
PceI AGGCCT 1 cut(s) 3190
PciI ACATGT 1 cut(s) 3733
PciSI GCTCTTC 2 cut(s) 1968, 2769
PctI GAATGC 1 cut(s) 3042
PdmI GAANNNNTTC 3 cut(s) 1408, 3040, 3693
PinAI ACCGGT 1 cut(s) 513
PleI GAGTC 6 cut(s) 1218, 1773, 2704, 3219, 3454, 3860
PpsI GAGTC 6 cut(s) 1218, 1773, 2704, 3219, 3454, 3860
Ppu21I YACGTR 1 cut(s) 203
PpuMI RGGWCCY 2 cut(s) 245, 912
PscI ACATGT 1 cut(s) 3733
PshAI GACNNNNGTC 1 cut(s) 3661
Psp124BI GAGCTC 1 cut(s) 1911
Psp5II RGGWCCY 2 cut(s) 245, 912
Psp6I CCWGG 3 cut(s) 2984, 3947, 4396
PspFI CCCAGC 4 cut(s) 441, 1040, 1194, 2358
PspGI CCWGG 3 cut(s) 2984, 3947, 4396
PspN4I GGNNCC 8 cut(s) 225, 257, 914, 1240, 2623, 3247, 3414, 3538
PspPI GGNCC 9 cut(s) 245, 912, 1846, 2315, 3274, 3631, 3854, 4104, 4112
PspPPI RGGWCCY 2 cut(s) 245, 912
PspXI VCTCGAGB 1 cut(s) 4254
PstI CTGCAG 3 cut(s) 535, 1571, 2065
PstNI CAGNNNCTG 3 cut(s) 248, 2381, 4184
PsuI RGATCY 6 cut(s) 320, 1370, 1456, 2881, 3139, 4357
PvuII CAGCTG 3 cut(s) 791, 2060, 2786
RsaI GTAC 8 cut(s) 88, 304, 434, 858, 1329, 1758, 1770, 3732
RsaNI GTAC 8 cut(s) 87, 303, 433, 857, 1328, 1757, 1769, 3731
RseI CAYNNNNRTG 2 cut(s) 4235, 4376
SacI GAGCTC 1 cut(s) 1911
SalI GTCGAC 2 cut(s) 3581, 3654
SapI GCTCTTC 2 cut(s) 1968, 2769
SaqAI TTAA 8 cut(s) 116, 299, 371, 659, 905, 1532, 1617, 2741
Sau96I GGNCC 9 cut(s) 245, 912, 1846, 2315, 3274, 3631, 3854, 4104, 4112
ScaI AGTACT 1 cut(s) 88
SchI GAGTC 6 cut(s) 1219, 1773, 2705, 3220, 3455, 3860
ScrFI CCNGG 4 cut(s) 2986, 3453, 3949, 4398
SduI GDGCHC 3 cut(s) 1642, 1911, 3628
SfcI CTRYAG 4 cut(s) 249, 531, 1567, 2061
Sfr274I CTCGAG 3 cut(s) 2109, 4254, 4323
SinI GGWCC 3 cut(s) 245, 912, 1846
SlaI CTCGAG 3 cut(s) 2109, 4254, 4323
SmiMI CAYNNNNRTG 2 cut(s) 4235, 4376
SmlI CTYRAG 7 cut(s) 115, 298, 1307, 2109, 4171, 4254, 4323
SmoI CTYRAG 7 cut(s) 115, 298, 1307, 2109, 4171, 4254, 4323
SnaBI TACGTA 1 cut(s) 203
SpeI ACTAGT 2 cut(s) 2477, 3473
SphI GCATGC 1 cut(s) 174
SseBI AGGCCT 1 cut(s) 3190
SsiI CCGC 6 cut(s) 55, 959, 1439, 1594, 1849, 2873
SspI AATATT 4 cut(s) 309, 1653, 2367, 2921
SspMI CTAG 9 cut(s) 606, 860, 1037, 1368, 2478, 2844, 2925, 3003, 3474
SstI GAGCTC 1 cut(s) 1911
StuI AGGCCT 1 cut(s) 3190
StyD4I CCNGG 4 cut(s) 2984, 3451, 3947, 4396
StyI CCWWGG 5 cut(s) 812, 3002, 3827, 3857, 4449
TaiI ACGT 6 cut(s) 205, 1774, 2904, 2953, 4151, 4317
TatI WGTACW 7 cut(s) 86, 302, 432, 856, 1327, 1756, 3730
TauI GCSGC 2 cut(s) 962, 1442
Tru1I TTAA 8 cut(s) 116, 299, 371, 659, 905, 1532, 1617, 2741
Tru9I TTAA 8 cut(s) 116, 299, 371, 659, 905, 1532, 1617, 2741
TseFI GTSAC 3 cut(s) 472, 751, 3447
Tsp45I GTSAC 3 cut(s) 472, 751, 3447
TspGWI ACGGA 2 cut(s) 2275, 3335
Vha464I CTTAAG 2 cut(s) 115, 298
VpaK11BI GGWCC 3 cut(s) 245, 912, 1846
XapI RAATTY 6 cut(s) 264, 1218, 1486, 1703, 2181, 3963
XbaI TCTAGA 2 cut(s) 605, 2843
XceI RCATGY 3 cut(s) 174, 3737, 3914
XcmI CCANNNNNNNNNTGG 2 cut(s) 466, 495
XhoI CTCGAG 3 cut(s) 2109, 4254, 4323
XmaJI CCTAGG 1 cut(s) 3002
XmiI GTMKAC 3 cut(s) 481, 3582, 3655
XmnI GAANNNNTTC 3 cut(s) 1408, 3040, 3693
XspI CTAG 9 cut(s) 606, 860, 1037, 1368, 2478, 2844, 2925, 3003, 3474
ZrmI AGTACT 1 cut(s) 88
Zsp2I ATGCAT 1 cut(s) 2709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.