FvH4_4g01200

Vegetative cell wall protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0007445
Physical Location & Seq
Forward (+)
1098638 .. 1101549
2912 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g01200.t2

Sequence Viewer

Length: 1485 bp
ATGGCATCACAACCAGCTCGTCCTTGGTTTAGACTAAGCTCCTTGGCGCGTCCAGCAGGGCCTGGTCCAACCCCCACTCCTGCACCATCCCCGGCACCAATCGCTGCCCCTCCCCCTGCAGCTCTTGTTCGACCTACATTGAGGCCACTAGCTCCTCCTCCTCCTGCTGAACCCAATCCACCACCACCAGTGGCCCGAGCTCCACCACCGCCACAAGCAGCAGCCCCAACTCCATCTCCCCCGCAACCAGCAGCCCCAACTCCAACTCCCCCAGCACCTGCTACCGCTGCACCACCCCCACCAGTTGCCGCTGCTCCAGCACCACCACCACCACCTCCTGCTGTGATTAGTACTACTGCATCTGTTCCATCATCACCAGCTCGTGGCATTATTTCTGCTCCCTCAGCAGCATCCCCTCCTGCTACTGCAATTGCCATCACATCTTCCGCTTCATCTTCAGTACCGCCATCTCCTGGAAACAAGGTCACCCCATCATCAACATCAGGGGTGCCATCTCCTAGAAACAGGGTCACTACTTCACAGCCCAACCCTCCAGCCTCCAGAGCAGTACCACCACCACAGAATTCTCCACCAAAACCCACAGCCACTGCAAATTTAGTGCCACCACAAACTTATTCTCCTCCTAAACCAAGGAGTACAACCATTTCATCAGCCAGTTCTGCTCGGGAGCCTAGTCCCAATTACAATCCATCTCCCAGAACAATTAAGCCGGTGAACAATACCCCGCCTCAGTCACCTAACAAGGCCAAACCTACTGCCCCGCCACCCTCTCCTCTCAACCTGCCACCATCTATGTTAAGGTCCAGCAATGCAGATCAAACTGATCAAAGGATTCCATTAGAGGCTGAGCAAAAGACAGTGCTGGTCCAAAAGACCATTGATCAAAAGTCCAATTCCTGGTTCAGTAGTGGCGGTTCAAAGCAGGATCATGACTTTAATAGTGAGTCCCAAAAGCCTAATCATTATGGCCACCATGCAAAACATGAAGAGAAAATCAACCGGAAGAAGTTATCATCTGATTCTGAGGAGGCTCTAGGCAAGAGGGTCATAACTATTGCAGGTGAAAACAAAGGGGCTTATATGGAACTCATTCGATCCCCAAATAAACATCATGAGCAACATGGCGAGCACTCACATTTTCTTCACAGGAAAGGCAACAATGGTAAGTCGGTGCAGACAGTTGGGAGTCATCAATCTGATAAAAGCTCCAGCAGCAGCAGCAGTGGCGAGGAAGGCAAGCCAAAAATGAAGGACAGAATTCACCGCAAAGGACTCAAGGCAGGGAACTCATCACAGCTGCCACTGAGTTCATTCACAAATAGCAATGTTCAAGGTATCAACAACTCCATTATGTACAACAGTTCCTGCACTCACCACGACCCAGGAGTGCACCTTGCTCTCACTAGAAAGCCCAGTGGGGATGGATTCGACGTCACACACCATGTCAATGGCCGCCACAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

495

Amino Acids

51.4

Weight (kDa)

10.32

Isoelectric Point (pI)

79.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 286, 1070
AatII GACGTC 1 cut(s) 1455
Acc36I ACCTGC 3 cut(s) 286, 810, 1070
AccB1I GGYRCC 2 cut(s) 94, 508
AccB7I CCANNNNNTGG 3 cut(s) 383, 473, 918
AccII CGCG 1 cut(s) 49
AclWI GGATC 2 cut(s) 954, 1110
AcoI YGGCCR 2 cut(s) 988, 1471
AcsI RAATTY 3 cut(s) 583, 613, 1278
AcuI CTGAAG 1 cut(s) 441
AcyI GRCGYC 1 cut(s) 1452
AfaI GTAC 5 cut(s) 352, 462, 570, 658, 1376
AfiI CCNNNNNNNGG 3 cut(s) 383, 473, 918
AgsI TTSAA 2 cut(s) 939, 1352
AjnI CCWGG 4 cut(s) 61, 472, 917, 1402
AjuI GAANNNNNNNTTGG 2 cut(s) 905, 937
AluBI AGCT 8 cut(s) 17, 39, 122, 152, 200, 380, 1227, 1318
AluI AGCT 8 cut(s) 17, 39, 122, 152, 200, 380, 1227, 1318
Alw21I GWGCWC 3 cut(s) 202, 1152, 1413
Alw44I GTGCAC 1 cut(s) 1409
AlwI GGATC 2 cut(s) 954, 1110
AlwNI CAGNNNCTG 4 cut(s) 62, 278, 608, 1386
Ama87I CYCGRG 2 cut(s) 195, 684
AoxI GGCC 6 cut(s) 59, 143, 192, 765, 988, 1471
ApaLI GTGCAC 1 cut(s) 1409
ApoI RAATTY 3 cut(s) 583, 613, 1278
Asp700I GAANNNNTTC 1 cut(s) 1110
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 5 cut(s) 59, 65, 193, 822, 886
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 7 cut(s) 366, 478, 745, 747, 1094, 1274, 1385
AvaI CYCGRG 2 cut(s) 195, 684
AvaII GGWCC 3 cut(s) 65, 822, 886
BaeGI GKGCMC 1 cut(s) 1413
BalI TGGCCA 1 cut(s) 990
BanI GGYRCC 2 cut(s) 94, 508
BanII GRGCYC 1 cut(s) 202
BauI CACGAG 1 cut(s) 381
Bbv12I GWGCWC 3 cut(s) 202, 1152, 1413
BbvCI CCTCAGC 1 cut(s) 403
BciT130I CCWGG 4 cut(s) 63, 474, 919, 1404
BclI TGATCA 2 cut(s) 844, 901
BcnI CCSGG 1 cut(s) 92
BfaI CTAG 5 cut(s) 149, 519, 693, 1055, 1425
BfmI CTRYAG 1 cut(s) 117
BfuAI ACCTGC 3 cut(s) 286, 810, 1070
BlpI GCTNAGC 1 cut(s) 867
BmcAI AGTACT 1 cut(s) 352
Bme1390I CCNGG 5 cut(s) 63, 92, 474, 919, 1404
Bme18I GGWCC 3 cut(s) 65, 822, 886
BmeT110I CYCGRG 2 cut(s) 195, 684
BmgT120I GGNCC 5 cut(s) 59, 65, 193, 822, 886
BmiI GGNNCC 3 cut(s) 96, 510, 690
BmrFI CCNGG 5 cut(s) 63, 92, 474, 919, 1404
BmrI ACTGGG 1 cut(s) 1428
BmsI GCATC 3 cut(s) 14, 368, 419
BmuI ACTGGG 1 cut(s) 1428
BpmI CTGGAG 4 cut(s) 300, 537, 544, 1213
Bpu10I CCTNAGC 1 cut(s) 403
Bpu1102I GCTNAGC 1 cut(s) 867
BpuEI CTTGAG 1 cut(s) 1280
BpuMI CCSGG 1 cut(s) 92
BsaHI GRCGYC 1 cut(s) 1452
BsaJI CCNNGG 5 cut(s) 23, 42, 90, 650, 1402
BsaWI WCCGGW 1 cut(s) 1020
BsaXI ACNNNNNCTCC 8 cut(s) 61, 91, 220, 250, 250, 280, 622, 652
Bsc4I CCNNNNNNNGG 3 cut(s) 383, 473, 918
Bse118I RCCGGY 1 cut(s) 730
Bse1I ACTGG 4 cut(s) 188, 302, 675, 1434
Bse3DI GCAATG 2 cut(s) 835, 1351
BseBI CCWGG 4 cut(s) 63, 474, 919, 1404
BseDI CCNNGG 5 cut(s) 23, 42, 90, 650, 1402
BseGI GGATG 3 cut(s) 86, 410, 1447
BseLI CCNNNNNNNGG 3 cut(s) 383, 473, 918
BseMI GCAATG 2 cut(s) 835, 1351
BseMII CTCAG 5 cut(s) 417, 764, 858, 1035, 1316
BseNI ACTGG 4 cut(s) 188, 302, 675, 1434
BseRI GAGGAG 6 cut(s) 144, 147, 150, 630, 783, 1061
BseSI GKGCMC 1 cut(s) 1413
BseYI CCCAGC 1 cut(s) 271
BsgI GTGCAG 4 cut(s) 66, 273, 1214, 1372
Bsh1236I CGCG 1 cut(s) 49
BshFI GGCC 6 cut(s) 61, 145, 194, 767, 990, 1473
BshNI GGYRCC 2 cut(s) 94, 508
BsiHKAI GWGCWC 3 cut(s) 202, 1152, 1413
BsiHKCI CYCGRG 2 cut(s) 195, 684
BsiSI CCGG 3 cut(s) 92, 731, 1021
BslFI GGGAC 2 cut(s) 681, 952
BslI CCNNNNNNNGG 3 cut(s) 383, 473, 918
BsmFI GGGAC 2 cut(s) 681, 952
BsnI GGCC 6 cut(s) 61, 145, 194, 767, 990, 1473
BsoBI CYCGRG 2 cut(s) 195, 684
Bsp1286I GDGCHC 3 cut(s) 202, 1152, 1413
Bsp1407I TGTACA 1 cut(s) 1374
Bsp143I GATC 5 cut(s) 835, 844, 901, 946, 1115
Bsp1720I GCTNAGC 1 cut(s) 867
BspANI GGCC 6 cut(s) 61, 145, 194, 767, 990, 1473
BspCNI CTCAG 5 cut(s) 416, 763, 859, 1036, 1317
BspFNI CGCG 1 cut(s) 49
BspHI TCATGA 2 cut(s) 949, 1132
BspLI GGNNCC 3 cut(s) 96, 510, 690
BspMAI CTGCAG 1 cut(s) 121
BspMI ACCTGC 3 cut(s) 286, 810, 1070
BspPI GGATC 2 cut(s) 954, 1110
BspT107I GGYRCC 2 cut(s) 94, 508
BsrDI GCAATG 2 cut(s) 835, 1351
BsrFI RCCGGY 1 cut(s) 730
BsrGI TGTACA 1 cut(s) 1374
BsrI ACTGG 4 cut(s) 188, 302, 675, 1434
BssAI RCCGGY 1 cut(s) 730
BssECI CCNNGG 5 cut(s) 23, 42, 90, 650, 1402
BssMI GATC 5 cut(s) 835, 844, 901, 946, 1115
BssNI GRCGYC 1 cut(s) 1452
BssSI CACGAG 1 cut(s) 381
BssT1I CCWWGG 3 cut(s) 23, 42, 650
Bst2BI CACGAG 1 cut(s) 381
Bst2UI CCWGG 4 cut(s) 63, 474, 919, 1404
Bst4CI ACNGT 4 cut(s) 880, 1201, 1382, 1481
Bst6I CTCTTC 1 cut(s) 1002
BstACI GRCGYC 1 cut(s) 1452
BstAUI TGTACA 1 cut(s) 1374
BstC8I GCNNGC 2 cut(s) 1148, 1259
BstDEI CTNAG 6 cut(s) 35, 403, 750, 867, 1044, 1325
BstEII GGTNACC 1 cut(s) 484
BstF5I GGATG 3 cut(s) 86, 410, 1447
BstFNI CGCG 1 cut(s) 49
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 5 cut(s) 838, 847, 904, 949, 1118
BstMBI GATC 5 cut(s) 835, 844, 901, 946, 1115
BstNI CCWGG 4 cut(s) 63, 474, 919, 1404
BstPI GGTNACC 1 cut(s) 484
BstSCI CCNGG 5 cut(s) 61, 90, 472, 917, 1402
BstSFI CTRYAG 1 cut(s) 117
BstSLI GKGCMC 1 cut(s) 1413
BstUI CGCG 1 cut(s) 49
BstXI CCANNNNNNTGG 1 cut(s) 1469
BsuRI GGCC 6 cut(s) 61, 145, 194, 767, 990, 1473
BtsCI GGATG 3 cut(s) 86, 410, 1447
BtsI GCAGTG 2 cut(s) 606, 1249
BtsIMutI CAGTG 6 cut(s) 195, 606, 885, 1249, 1322, 1441
BveI ACCTGC 3 cut(s) 286, 810, 1070
Cac8I GCNNGC 2 cut(s) 1148, 1259
CaiI CAGNNNCTG 4 cut(s) 62, 278, 608, 1386
CciI TCATGA 2 cut(s) 949, 1132
CfoI GCGC 1 cut(s) 49
Cfr10I RCCGGY 1 cut(s) 730
Cfr13I GGNCC 5 cut(s) 59, 65, 193, 822, 886
CseI GACGC 1 cut(s) 38
Csp6I GTAC 5 cut(s) 351, 461, 569, 657, 1375
CviAII CATG 6 cut(s) 950, 995, 1004, 1133, 1142, 1463
CviQI GTAC 5 cut(s) 351, 461, 569, 657, 1375
DdeI CTNAG 6 cut(s) 35, 403, 750, 867, 1044, 1325
DpnI GATC 5 cut(s) 837, 846, 903, 948, 1117
DpnII GATC 5 cut(s) 835, 844, 901, 946, 1115
EaeI YGGCCR 2 cut(s) 988, 1471
Eam1104I CTCTTC 1 cut(s) 1002
EarI CTCTTC 1 cut(s) 1002
Ecl136II GAGCTC 1 cut(s) 200
Eco130I CCWWGG 3 cut(s) 23, 42, 650
Eco24I GRGCYC 1 cut(s) 202
Eco47I GGWCC 3 cut(s) 65, 822, 886
Eco53kI GAGCTC 1 cut(s) 200
Eco57I CTGAAG 1 cut(s) 441
Eco88I CYCGRG 2 cut(s) 195, 684
Eco91I GGTNACC 1 cut(s) 484
EcoICRI GAGCTC 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 59
EcoO65I GGTNACC 1 cut(s) 484
EcoRI GAATTC 2 cut(s) 583, 1278
EcoRII CCWGG 4 cut(s) 61, 472, 917, 1402
EcoT14I CCWWGG 3 cut(s) 23, 42, 650
EcoT38I GRGCYC 1 cut(s) 202
ErhI CCWWGG 3 cut(s) 23, 42, 650
FaeI CATG 6 cut(s) 953, 998, 1007, 1136, 1145, 1466
FaqI GGGAC 2 cut(s) 681, 952
FatI CATG 6 cut(s) 949, 994, 1003, 1132, 1141, 1462
FauI CCCGC 3 cut(s) 249, 753, 789
FbaI TGATCA 2 cut(s) 844, 901
FokI GGATG 3 cut(s) 73, 397, 1454
FriOI GRGCYC 1 cut(s) 202
FspBI CTAG 5 cut(s) 149, 519, 693, 1055, 1425
GlaI GCGC 1 cut(s) 48
GsaI CCCAGC 1 cut(s) 275
GsuI CTGGAG 4 cut(s) 300, 537, 544, 1213
HaeIII GGCC 6 cut(s) 61, 145, 194, 767, 990, 1473
HapII CCGG 3 cut(s) 92, 731, 1021
HgaI GACGC 1 cut(s) 38
HhaI GCGC 1 cut(s) 49
Hin1I GRCGYC 1 cut(s) 1452
Hin1II CATG 6 cut(s) 953, 998, 1007, 1136, 1145, 1466
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HinfI GANTC 6 cut(s) 853, 965, 1040, 1207, 1293, 1446
HpaII CCGG 3 cut(s) 92, 731, 1021
HphI GGTGA 7 cut(s) 366, 478, 745, 747, 1094, 1274, 1385
Hpy166II GTNNAC 2 cut(s) 736, 1411
Hpy188I TCNGA 3 cut(s) 1039, 1045, 1219
Hpy188III TCNNGA 4 cut(s) 561, 686, 950, 1133
Hpy8I GTNNAC 2 cut(s) 736, 1411
Hpy99I CGWCG 1 cut(s) 1454
HpyAV CCTTC 2 cut(s) 1247, 1264
HpyCH4III ACNGT 4 cut(s) 880, 1201, 1382, 1481
HpyCH4IV ACGT 1 cut(s) 1452
HpyF3I CTNAG 6 cut(s) 35, 403, 750, 867, 1044, 1325
HpySE526I ACGT 1 cut(s) 1452
Hsp92I GRCGYC 1 cut(s) 1452
Hsp92II CATG 6 cut(s) 953, 998, 1007, 1136, 1145, 1466
HspAI GCGC 1 cut(s) 47
Ksp22I TGATCA 2 cut(s) 844, 901
Kzo9I GATC 5 cut(s) 835, 844, 901, 946, 1115
LmnI GCTCC 7 cut(s) 44, 157, 205, 319, 403, 688, 1232
LweI GCATC 3 cut(s) 14, 368, 419
MaeI CTAG 5 cut(s) 149, 519, 693, 1055, 1425
MaeII ACGT 1 cut(s) 1452
MaeIII GTNAC 4 cut(s) 484, 529, 753, 1453
MalI GATC 5 cut(s) 837, 846, 903, 948, 1117
MboI GATC 5 cut(s) 835, 844, 901, 946, 1115
MboII GAAGA 5 cut(s) 435, 447, 1019, 1036, 1154
MfeI CAATTG 1 cut(s) 429
MhlI GDGCHC 3 cut(s) 202, 1152, 1413
MlsI TGGCCA 1 cut(s) 990
MluCI AATT 7 cut(s) 429, 583, 613, 700, 723, 913, 1278
MluNI TGGCCA 1 cut(s) 990
MlyI GAGTC 3 cut(s) 974, 1216, 1287
MmeI TCCRAC 2 cut(s) 92, 287
Mox20I TGGCCA 1 cut(s) 990
MroXI GAANNNNTTC 1 cut(s) 1110
MscI TGGCCA 1 cut(s) 990
MseI TTAA 4 cut(s) 726, 818, 957, 1483
MslI CAYNNNNRTG 1 cut(s) 1467
Msp20I TGGCCA 1 cut(s) 990
MspA1I CMGCKG 3 cut(s) 287, 311, 1318
MspI CCGG 3 cut(s) 92, 731, 1021
MspR9I CCNGG 5 cut(s) 63, 92, 474, 919, 1404
MunI CAATTG 1 cut(s) 429
MvaI CCWGG 4 cut(s) 63, 474, 919, 1404
MvnI CGCG 1 cut(s) 49
NciI CCSGG 1 cut(s) 92
NdeII GATC 5 cut(s) 835, 844, 901, 946, 1115
NlaIII CATG 6 cut(s) 953, 998, 1007, 1136, 1145, 1466
NlaIV GGNNCC 3 cut(s) 96, 510, 690
NmuCI GTSAC 4 cut(s) 484, 529, 753, 1453
PagI TCATGA 2 cut(s) 949, 1132
PaqCI CACCTGC 2 cut(s) 286, 1070
PdmI GAANNNNTTC 1 cut(s) 1110
PfeI GAWTC 3 cut(s) 853, 1040, 1446
PflMI CCANNNNNTGG 3 cut(s) 383, 473, 918
PfoI TCCNGGA 1 cut(s) 472
PleI GAGTC 3 cut(s) 973, 1215, 1287
PpsI GAGTC 3 cut(s) 973, 1215, 1287
Psp124BI GAGCTC 1 cut(s) 202
Psp6I CCWGG 4 cut(s) 61, 472, 917, 1402
PspEI GGTNACC 1 cut(s) 484
PspFI CCCAGC 1 cut(s) 271
PspGI CCWGG 4 cut(s) 61, 472, 917, 1402
PspN4I GGNNCC 3 cut(s) 96, 510, 690
PspPI GGNCC 5 cut(s) 59, 65, 193, 822, 886
PsrI GAACNNNNNNTAC 2 cut(s) 919, 951
PstI CTGCAG 1 cut(s) 121
PstNI CAGNNNCTG 4 cut(s) 62, 278, 608, 1386
PvuII CAGCTG 1 cut(s) 1318
RsaI GTAC 5 cut(s) 352, 462, 570, 658, 1376
RsaNI GTAC 5 cut(s) 351, 461, 569, 657, 1375
RseI CAYNNNNRTG 1 cut(s) 1467
SacI GAGCTC 1 cut(s) 202
SaqAI TTAA 4 cut(s) 726, 818, 957, 1483
Sau3AI GATC 5 cut(s) 835, 844, 901, 946, 1115
Sau96I GGNCC 5 cut(s) 59, 65, 193, 822, 886
ScaI AGTACT 1 cut(s) 352
SchI GAGTC 3 cut(s) 974, 1216, 1287
ScrFI CCNGG 5 cut(s) 63, 92, 474, 919, 1404
SduI GDGCHC 3 cut(s) 202, 1152, 1413
SfaNI GCATC 3 cut(s) 14, 368, 419
SfcI CTRYAG 1 cut(s) 117
SinI GGWCC 3 cut(s) 65, 822, 886
SmiMI CAYNNNNRTG 1 cut(s) 1467
SmlI CTYRAG 1 cut(s) 1295
SmoI CTYRAG 1 cut(s) 1295
Sse9I AATT 7 cut(s) 429, 583, 613, 700, 723, 913, 1278
SspMI CTAG 5 cut(s) 149, 519, 693, 1055, 1425
SstI GAGCTC 1 cut(s) 202
StyD4I CCNGG 5 cut(s) 61, 90, 472, 917, 1402
StyI CCWWGG 3 cut(s) 23, 42, 650
TaaI ACNGT 4 cut(s) 880, 1201, 1382, 1481
TaiI ACGT 1 cut(s) 1455
TaqI TCGA 3 cut(s) 130, 1114, 1449
TasI AATT 7 cut(s) 429, 583, 613, 700, 723, 913, 1278
TatI WGTACW 3 cut(s) 350, 656, 1374
TauI GCSGC 2 cut(s) 311, 1476
TfiI GAWTC 3 cut(s) 853, 1040, 1446
Tru1I TTAA 4 cut(s) 726, 818, 957, 1483
Tru9I TTAA 4 cut(s) 726, 818, 957, 1483
TscAI CASTG 6 cut(s) 195, 613, 885, 1249, 1329, 1441
TseFI GTSAC 4 cut(s) 484, 529, 753, 1453
Tsp45I GTSAC 4 cut(s) 484, 529, 753, 1453
TspDTI ATGAA 5 cut(s) 441, 657, 1020, 1283, 1320
TspRI CASTG 6 cut(s) 195, 613, 885, 1249, 1329, 1441
Van91I CCANNNNNTGG 3 cut(s) 383, 473, 918
VneI GTGCAC 1 cut(s) 1409
VpaK11BI GGWCC 3 cut(s) 65, 822, 886
XapI RAATTY 3 cut(s) 583, 613, 1278
XcmI CCANNNNNNNNNTGG 1 cut(s) 21
XmnI GAANNNNTTC 1 cut(s) 1110
XspI CTAG 5 cut(s) 149, 519, 693, 1055, 1425
ZraI GACGTC 1 cut(s) 1453
ZrmI AGTACT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.