FvH4_4g06290

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014710
Physical Location & Seq
Forward (+)
5608728 .. 5609906
1179 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g06290.t1

Sequence Viewer

Length: 1179 bp
ATGGTTAGTAGAGGAAGAAAAAGCTCCAAACTCATAGCTTCTTCTCGTGCAGCTGAAGAAGTGGTTAAGTCAACAGATCTCTTGAGTGAGATCCTACTGCGCCTCCCAGCCAAGCCAGTTCTCAAGTTCAAATTAGTTTCAAAACAATGGTGCTCCATAATCTGCACCCCAGAATTTACCGTCCTTTATGCCAAAAGAAACCCAAACCCTCACCTAGGGTTTATGTTGCACGCTCGACTTGACTTCCAACCACAACCTCGTGCTATCTTCCTTGATCTGGGTGGCTATCAATATGATAAACTCCCAAATTTTCTTAACATCTCAAAAATGGAGATTCACCACGTCTGTAATGGCTTGTTGTGCTATTCCAGACCTCATCTGAGTTCCCCTAACATCACTTCATATTATATTTGTAATCATTCATCAGGGCAATCCGAATTCCAAAGGGTCCTTGTAAGTCCAGACAATGAAACAGTGAAGGCTTTCAATGTGGCTTTTGATCCCAAGCTATCACCACATTACAGGCTCGTTTTTGTGAGAGAATTGCCTGTGTTAGACGAGACCCGTCTCATCAAAATTGATATCTACTCGTCTAAGACCAAGTCTTGGAGAGCCTCCAGGAAGGGGATTTACATTTTGCAATACGATATAGGGTTTCATGTTTCAGTTTACTGGAAGGATGCAATGTACTGGTACAATGAGAAGAATCAGTCTTTGGCATATTTCGATTTCAATGACGACACCGTGGAGATTTTGCCAAAGCCAAAAGTTACTGGAGGAACCATGAAATGTTACTATCTCGGCGAATCGGGCGGGCATTTGCATCTTGTGGGAGCCTGGAGACCTTGGACTAGTTTGTTGACTGTCTTCGAACTGAAGGAAGATAGGTCTGGGTGGAATGTGAAGTATCATGGTGATTTTCGTGCTTTAGCTAATTCCTTTCCGAAGATGCTTACTAAGAATCCAAGAAGGCCTTATATGTTTTCTGTGGTGAACCTGGTAAGAGGTGAAAGAGAAGGGGAGTTGATCATTGTGATGATAATCCCTGAGGGTGTGATTTCTTATAATATCCGGGAGAGGAGCTTGAAGAAACTCTTGGATCTGGCTCTGAGTGAAACTTACATGGAACAGTTGACGTTTAGGTCTCATCAATACATTGAGACCCTTGCTTCGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

393

Amino Acids

45.34

Weight (kDa)

9.3

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 26 - 60 4.9e-07 F-box domain
FBA_1 PF07734 109 - 254 7.6e-08 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014710)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06290 FvH4_4g06330
malus_domestica MD13G1235000.v1.1
prunus_persica Prupe.1G066100_v2.0.a1 Prupe.1G066200_v2.0.a1
pyrus_communis pycom13g20750
rosa_chinensis RchiOBHm_Chr4g0398011
rosa_laevigata RLG00000014125
rosa_roxburghii Rroxscaffold_5G00342970
rosa_rugosa Rorug04G0005500 Rorug06G0022700
rosa_samantha Rh4AG082200 Rh4BG079700 Rh4DG075100 Rh6BG144300 Rh6DG128700
rosa_wichuraiana Rw4G006860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1065
AasI GACNNNNNNGTC 1 cut(s) 1141
AccB7I CCANNNNNTGG 1 cut(s) 606
AciI CCGC 1 cut(s) 813
AclWI GGATC 3 cut(s) 85, 494, 1107
AcsI RAATTY 3 cut(s) 173, 307, 437
AcuI CTGAAG 2 cut(s) 75, 896
AfaI GTAC 2 cut(s) 689, 695
AfiI CCNNNNNNNGG 4 cut(s) 215, 277, 606, 624
AgsI TTSAA 5 cut(s) 130, 141, 487, 733, 1087
AhlI ACTAGT 1 cut(s) 851
AjiI CACGTC 1 cut(s) 343
AjnI CCWGG 3 cut(s) 617, 836, 996
AjuI GAANNNNNNNTTGG 4 cut(s) 698, 730, 1079, 1111
AluBI AGCT 6 cut(s) 24, 38, 53, 508, 932, 1083
AluI AGCT 6 cut(s) 24, 38, 53, 508, 932, 1083
Alw21I GWGCWC 1 cut(s) 155
Alw26I GTCTC 5 cut(s) 554, 572, 835, 1149, 1154
AlwI GGATC 3 cut(s) 85, 494, 1107
AoxI GGCC 1 cut(s) 971
ApeKI GCWGC 1 cut(s) 50
ApoI RAATTY 3 cut(s) 173, 307, 437
ArsI GACNNNNNNTTYG 1 cut(s) 1153
Asp700I GAANNNNTTC 1 cut(s) 482
AspA2I CCTAGG 1 cut(s) 214
AspLEI GCGC 1 cut(s) 102
AspS9I GGNCC 1 cut(s) 448
AsuC2I CCSGG 1 cut(s) 1073
AsuHPI GGTGA 6 cut(s) 203, 329, 504, 926, 1003, 1019
AsuII TTCGAA 1 cut(s) 870
AvaII GGWCC 1 cut(s) 448
AvrII CCTAGG 1 cut(s) 214
AxyI CCTNAGG 1 cut(s) 1047
BarI GAAGNNNNNNTAC 2 cut(s) 614, 646
BauI CACGAG 2 cut(s) 45, 258
BbsI GAAGAC 1 cut(s) 859
Bbv12I GWGCWC 1 cut(s) 155
BbvI GCAGC 1 cut(s) 62
BciT130I CCWGG 3 cut(s) 619, 838, 998
BclI TGATCA 1 cut(s) 1026
BcnI CCSGG 1 cut(s) 1073
BcoDI GTCTC 5 cut(s) 554, 572, 835, 1149, 1154
BcuI ACTAGT 1 cut(s) 851
BfaI CTAG 2 cut(s) 215, 852
BglII AGATCT 1 cut(s) 76
BisI GCNGC 1 cut(s) 51
BlnI CCTAGG 1 cut(s) 214
BlsI GCNGC 1 cut(s) 52
Bme1390I CCNGG 4 cut(s) 619, 838, 998, 1073
Bme18I GGWCC 1 cut(s) 448
BmgBI CACGTC 1 cut(s) 343
BmgT120I GGNCC 1 cut(s) 448
BmiI GGNNCC 3 cut(s) 449, 781, 835
BmrFI CCNGG 4 cut(s) 619, 838, 998, 1073
BmsI GCATC 3 cut(s) 670, 832, 939
BpiI GAAGAC 1 cut(s) 859
BpmI CTGGAG 3 cut(s) 601, 795, 859
Bpu14I TTCGAA 1 cut(s) 870
BpuEI CTTGAG 2 cut(s) 103, 107
BpuMI CCSGG 1 cut(s) 1073
BsaBI GATNNNNATC 1 cut(s) 1040
BsaI GGTCTC 4 cut(s) 554, 835, 1149, 1154
BsaJI CCNNGG 3 cut(s) 214, 744, 845
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bsc4I CCNNNNNNNGG 4 cut(s) 215, 277, 606, 624
Bse1I ACTGG 4 cut(s) 116, 677, 695, 778
Bse21I CCTNAGG 1 cut(s) 1047
Bse3DI GCAATG 1 cut(s) 690
Bse8I GATNNNNATC 1 cut(s) 1040
BseBI CCWGG 3 cut(s) 619, 838, 998
BseDI CCNNGG 3 cut(s) 214, 744, 845
BseGI GGATG 1 cut(s) 685
BseJI GATNNNNATC 1 cut(s) 1040
BseLI CCNNNNNNNGG 4 cut(s) 215, 277, 606, 624
BseMI GCAATG 1 cut(s) 690
BseMII CTCAG 3 cut(s) 371, 1038, 1100
BseNI ACTGG 4 cut(s) 116, 677, 695, 778
BseRI GAGGAG 1 cut(s) 1093
BseXI GCAGC 1 cut(s) 62
BseYI CCCAGC 1 cut(s) 106
BsgI GTGCAG 2 cut(s) 69, 148
BshFI GGCC 1 cut(s) 973
BsiHKAI GWGCWC 1 cut(s) 155
BsiSI CCGG 1 cut(s) 1072
BslI CCNNNNNNNGG 4 cut(s) 215, 277, 606, 624
BsmAI GTCTC 5 cut(s) 554, 572, 835, 1149, 1154
BsmBI CGTCTC 1 cut(s) 572
BsnI GGCC 1 cut(s) 973
Bso31I GGTCTC 4 cut(s) 554, 835, 1149, 1154
Bsp119I TTCGAA 1 cut(s) 870
Bsp1286I GDGCHC 1 cut(s) 155
Bsp143I GATC 6 cut(s) 76, 90, 274, 499, 1026, 1099
BspACI CCGC 1 cut(s) 813
BspANI GGCC 1 cut(s) 973
BspCNI CTCAG 3 cut(s) 372, 1039, 1101
BspLI GGNNCC 3 cut(s) 449, 781, 835
BspPI GGATC 3 cut(s) 85, 494, 1107
BspT104I TTCGAA 1 cut(s) 870
BspTNI GGTCTC 4 cut(s) 554, 835, 1149, 1154
BsrDI GCAATG 1 cut(s) 690
BsrI ACTGG 4 cut(s) 116, 677, 695, 778
BssECI CCNNGG 3 cut(s) 214, 744, 845
BssMI GATC 6 cut(s) 76, 90, 274, 499, 1026, 1099
BssSI CACGAG 2 cut(s) 45, 258
BssT1I CCWWGG 2 cut(s) 214, 845
Bst2BI CACGAG 2 cut(s) 45, 258
Bst2UI CCWGG 3 cut(s) 619, 838, 998
Bst4CI ACNGT 5 cut(s) 181, 475, 745, 865, 1131
BstBI TTCGAA 1 cut(s) 870
BstC8I GCNNGC 2 cut(s) 231, 815
BstDEI CTNAG 5 cut(s) 380, 594, 957, 1047, 1109
BstDSI CCRYGG 1 cut(s) 744
BstENI CCTNNNNNAGG 1 cut(s) 213
BstF5I GGATG 1 cut(s) 685
BstHHI GCGC 1 cut(s) 102
BstKTI GATC 6 cut(s) 79, 93, 277, 502, 1029, 1102
BstMAI GTCTC 5 cut(s) 554, 572, 835, 1149, 1154
BstMBI GATC 6 cut(s) 76, 90, 274, 499, 1026, 1099
BstMWI GCNNNNNNNGC 2 cut(s) 360, 810
BstNI CCWGG 3 cut(s) 619, 838, 998
BstSCI CCNGG 4 cut(s) 617, 836, 996, 1071
BstV1I GCAGC 1 cut(s) 62
BstV2I GAAGAC 1 cut(s) 859
BstX2I RGATCY 3 cut(s) 76, 90, 1099
BstYI RGATCY 3 cut(s) 76, 90, 1099
Bsu36I CCTNAGG 1 cut(s) 1047
BsuRI GGCC 1 cut(s) 973
BtgI CCRYGG 1 cut(s) 744
BtrI CACGTC 1 cut(s) 343
BtsCI GGATG 1 cut(s) 685
BtsIMutI CAGTG 1 cut(s) 480
Cac8I GCNNGC 2 cut(s) 231, 815
CfoI GCGC 1 cut(s) 102
Cfr13I GGNCC 1 cut(s) 448
CsiI ACCWGGT 1 cut(s) 996
Csp6I GTAC 2 cut(s) 688, 694
CviAII CATG 4 cut(s) 659, 784, 911, 1123
CviQI GTAC 2 cut(s) 688, 694
DdeI CTNAG 5 cut(s) 380, 594, 957, 1047, 1109
DpnI GATC 6 cut(s) 78, 92, 276, 501, 1028, 1101
DpnII GATC 6 cut(s) 76, 90, 274, 499, 1026, 1099
DrdI GACNNNNNNGTC 1 cut(s) 1141
DseDI GACNNNNNNGTC 1 cut(s) 1141
Eco130I CCWWGG 2 cut(s) 214, 845
Eco147I AGGCCT 1 cut(s) 973
Eco31I GGTCTC 4 cut(s) 554, 835, 1149, 1154
Eco32I GATATC 1 cut(s) 583
Eco47I GGWCC 1 cut(s) 448
Eco57I CTGAAG 2 cut(s) 75, 896
Eco81I CCTNAGG 1 cut(s) 1047
EcoNI CCTNNNNNAGG 1 cut(s) 213
EcoO109I RGGNCCY 1 cut(s) 448
EcoRI GAATTC 1 cut(s) 437
EcoRII CCWGG 3 cut(s) 617, 836, 996
EcoRV GATATC 1 cut(s) 583
EcoT14I CCWWGG 2 cut(s) 214, 845
ErhI CCWWGG 2 cut(s) 214, 845
Esp3I CGTCTC 1 cut(s) 572
FaeI CATG 4 cut(s) 662, 787, 914, 1126
FalI AAGNNNNNCTT 4 cut(s) 958, 990, 1079, 1111
FatI CATG 4 cut(s) 658, 783, 910, 1122
FauI CCCGC 1 cut(s) 806
FbaI TGATCA 1 cut(s) 1026
Fnu4HI GCNGC 1 cut(s) 51
FokI GGATG 1 cut(s) 692
Fsp4HI GCNGC 1 cut(s) 51
FspBI CTAG 2 cut(s) 215, 852
GlaI GCGC 1 cut(s) 101
GluI GCNGC 1 cut(s) 51
GsaI CCCAGC 1 cut(s) 110
GsuI CTGGAG 3 cut(s) 601, 795, 859
HaeIII GGCC 1 cut(s) 973
HapII CCGG 1 cut(s) 1072
HhaI GCGC 1 cut(s) 102
Hin1II CATG 4 cut(s) 662, 787, 914, 1126
Hin6I GCGC 1 cut(s) 100
HinP1I GCGC 1 cut(s) 100
HincII GTYRAC 3 cut(s) 72, 861, 1134
HindII GTYRAC 3 cut(s) 72, 861, 1134
HinfI GANTC 4 cut(s) 334, 706, 806, 961
HpaII CCGG 1 cut(s) 1072
HphI GGTGA 6 cut(s) 203, 329, 504, 926, 1003, 1019
Hpy166II GTNNAC 5 cut(s) 72, 670, 861, 994, 1134
Hpy188I TCNGA 4 cut(s) 381, 436, 945, 1110
Hpy188III TCNNGA 3 cut(s) 82, 369, 461
Hpy8I GTNNAC 5 cut(s) 72, 670, 861, 994, 1134
HpyAV CCTTC 6 cut(s) 472, 616, 670, 871, 963, 1010
HpyCH4III ACNGT 5 cut(s) 181, 475, 745, 865, 1131
HpyCH4IV ACGT 2 cut(s) 342, 1136
HpyCH4V TGCA 6 cut(s) 50, 165, 229, 640, 683, 823
HpyF10VI GCNNNNNNNGC 2 cut(s) 360, 810
HpyF3I CTNAG 5 cut(s) 380, 594, 957, 1047, 1109
HpySE526I ACGT 2 cut(s) 342, 1136
Hsp92II CATG 4 cut(s) 662, 787, 914, 1126
HspAI GCGC 1 cut(s) 100
Ksp22I TGATCA 1 cut(s) 1026
Kzo9I GATC 6 cut(s) 76, 90, 274, 499, 1026, 1099
LmnI GCTCC 4 cut(s) 29, 158, 833, 1080
Lsp1109I GCAGC 1 cut(s) 62
LweI GCATC 3 cut(s) 670, 832, 939
MabI ACCWGGT 1 cut(s) 996
MaeI CTAG 2 cut(s) 215, 852
MaeII ACGT 2 cut(s) 342, 1136
MaeIII GTNAC 2 cut(s) 769, 791
MalI GATC 6 cut(s) 78, 92, 276, 501, 1028, 1101
MboI GATC 6 cut(s) 76, 90, 274, 499, 1026, 1099
MboII GAAGA 9 cut(s) 27, 33, 68, 259, 715, 859, 893, 958, 1099
MflI RGATCY 3 cut(s) 76, 90, 1099
MhlI GDGCHC 1 cut(s) 155
MluCI AATT 7 cut(s) 131, 173, 307, 437, 542, 576, 934
MmeI TCCRAC 1 cut(s) 271
MroXI GAANNNNTTC 1 cut(s) 482
MseI TTAA 2 cut(s) 66, 315
MslI CAYNNNNRTG 1 cut(s) 1034
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 1 cut(s) 1072
MspR9I CCNGG 4 cut(s) 619, 838, 998, 1073
MvaI CCWGG 3 cut(s) 619, 838, 998
MwoI GCNNNNNNNGC 2 cut(s) 360, 810
NciI CCSGG 1 cut(s) 1073
NdeII GATC 6 cut(s) 76, 90, 274, 499, 1026, 1099
NlaIII CATG 4 cut(s) 662, 787, 914, 1126
NlaIV GGNNCC 3 cut(s) 449, 781, 835
NmeAIII GCCGAG 1 cut(s) 780
NspV TTCGAA 1 cut(s) 870
PceI AGGCCT 1 cut(s) 973
PdmI GAANNNNTTC 1 cut(s) 482
PfeI GAWTC 4 cut(s) 334, 706, 806, 961
PflFI GACNNNGTC 1 cut(s) 601
PflMI CCANNNNNTGG 1 cut(s) 606
PfoI TCCNGGA 2 cut(s) 617, 1071
PkrI GCNGC 1 cut(s) 52
PpuMI RGGWCCY 1 cut(s) 448
PsiI TTATAA 1 cut(s) 1065
Psp5II RGGWCCY 1 cut(s) 448
Psp6I CCWGG 3 cut(s) 617, 836, 996
PspFI CCCAGC 1 cut(s) 106
PspGI CCWGG 3 cut(s) 617, 836, 996
PspN4I GGNNCC 3 cut(s) 449, 781, 835
PspPI GGNCC 1 cut(s) 448
PspPPI RGGWCCY 1 cut(s) 448
PsuI RGATCY 3 cut(s) 76, 90, 1099
PsyI GACNNNGTC 1 cut(s) 601
PvuII CAGCTG 1 cut(s) 53
RsaI GTAC 2 cut(s) 689, 695
RsaNI GTAC 2 cut(s) 688, 694
RseI CAYNNNNRTG 1 cut(s) 1034
SaqAI TTAA 2 cut(s) 66, 315
SatI GCNGC 1 cut(s) 51
Sau3AI GATC 6 cut(s) 76, 90, 274, 499, 1026, 1099
Sau96I GGNCC 1 cut(s) 448
ScrFI CCNGG 4 cut(s) 619, 838, 998, 1073
SduI GDGCHC 1 cut(s) 155
SexAI ACCWGGT 1 cut(s) 996
SfaNI GCATC 3 cut(s) 670, 832, 939
SfuI TTCGAA 1 cut(s) 870
SinI GGWCC 1 cut(s) 448
SmiMI CAYNNNNRTG 1 cut(s) 1034
SmlI CTYRAG 2 cut(s) 82, 122
SmoI CTYRAG 2 cut(s) 82, 122
SpeI ACTAGT 1 cut(s) 851
Sse9I AATT 7 cut(s) 131, 173, 307, 437, 542, 576, 934
SseBI AGGCCT 1 cut(s) 973
SsiI CCGC 1 cut(s) 813
SspMI CTAG 2 cut(s) 215, 852
StuI AGGCCT 1 cut(s) 973
StyD4I CCNGG 4 cut(s) 617, 836, 996, 1071
StyI CCWWGG 2 cut(s) 214, 845
TaaI ACNGT 5 cut(s) 181, 475, 745, 865, 1131
TaiI ACGT 2 cut(s) 345, 1139
TaqI TCGA 4 cut(s) 235, 726, 870, 1172
TasI AATT 7 cut(s) 131, 173, 307, 437, 542, 576, 934
TatI WGTACW 1 cut(s) 687
TfiI GAWTC 4 cut(s) 334, 706, 806, 961
Tru1I TTAA 2 cut(s) 66, 315
Tru9I TTAA 2 cut(s) 66, 315
TscAI CASTG 1 cut(s) 480
TseI GCWGC 1 cut(s) 50
TspDTI ATGAA 5 cut(s) 390, 411, 483, 647, 800
TspRI CASTG 1 cut(s) 480
Tth111I GACNNNGTC 1 cut(s) 601
Van91I CCANNNNNTGG 1 cut(s) 606
VpaK11BI GGWCC 1 cut(s) 448
XagI CCTNNNNNAGG 1 cut(s) 213
XapI RAATTY 3 cut(s) 173, 307, 437
XcmI CCANNNNNNNNNTGG 1 cut(s) 347
XmaJI CCTAGG 1 cut(s) 214
XmnI GAANNNNTTC 1 cut(s) 482
XspI CTAG 2 cut(s) 215, 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.