Rw4G006860

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014710
Physical Location & Seq
Forward (+)
14338317 .. 14339786
1470 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G006860.1

Sequence Viewer

Length: 1179 bp
ATGGCAAGTAGAGGAGGAAGAAAAGGGTCCAACATCAGAGCTTCTTCCAGTGCAGCTGAAGTGGGCAATTCCGAAGACCTCTTGAGCGAGATTCTAGTTCGCCTCCCAGCCATACCAATTCTCAGGTTCAAATTAGTTTCAAAACAATGGTACTCCATAATCTCTACCCAAAAATTCACAGTCACTTACGCCAAAAGAAACCCAAACCCTCGCTTGGGGTTTTTGTTGCATACTCGGCTTAATTTCCAACCATACCCACCTACTCTCTTCCTTTCTTTGGGTGGCTATCAATATGGCAAACACCCAAATTTCCTTGAAATCCCAAAACTTGAAATCCATCATGTCTGTAATGGGTTGTTATGCTGTTCTAAGCCTCATGTGCATGCTCCTGAAATCACTTCTTATTATGTTTGTAATCATTCTTCTGGGCAGTCAAAATGGCTTCGGGTTCTTACAAGTCCATGCATTGAACGTGTAAAGGCTTTTAATTTGGCCTTTGATCCTTTGAAATCACCTCATTACAGGCTCTTTTTTGTTAGTGAATTGTCTTCGTTAGATGAGTACCATTTGATCAAAATTGATATTTACTTATCCGAGACCGAGTCTTGGAGACCCTCCAAGAAGGGGGTTTACATTTTGCAATACGACATAGGGTTTCACATTGCCGTTTACTGGAAGGATGCAATTTACTGGTACAATCAGAAGAATCAGTCTTTGGCGTATTTCGATTTCGATGACGACAGCGTGGAGAGTTTGCCAAAGCCGCAAGTTTCTGCTGGAACCATGACTTATTACTATTTTGGCGAGTCGGGTGGGCATTTGCATCTTGTTGGAGCTTGTAGACCTTGGACTACATTGTTCACTGTCTTCGAAATGAAGGAAGATAGGTCTGGGTGGTTTGTGAAGTATAATGGTGATTTTGGTGCTTTAGCTGCTGCATTTCCGAAGATGGTTACTAATAAACCAAATAGGCCTTATGTGTTTTCTCTGGTGAACCTATTAAGAGGTGAAAAAGAAGAGGAGTTGATTATTGTGTTAACAATTCCCGAAGGTGTGATTGGGTATAATGTCCAGGATAAGACCTTTGAGAAGCTGTGCAATCTATCTTTGAGTGAACGTAAAATGCAGCAAATGACATTTAATGCTCATCAATATATTGAGACCGTTGCTTCAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

392

Amino Acids

44.92

Weight (kDa)

8.75

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 25 - 60 1.3e-07 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014710)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06290 FvH4_4g06330
malus_domestica MD13G1235000.v1.1
prunus_persica Prupe.1G066100_v2.0.a1 Prupe.1G066200_v2.0.a1
pyrus_communis pycom13g20750
rosa_chinensis RchiOBHm_Chr4g0398011
rosa_laevigata RLG00000014125
rosa_roxburghii Rroxscaffold_5G00342970
rosa_rugosa Rorug04G0005500 Rorug06G0022700
rosa_samantha Rh4AG082200 Rh4BG079700 Rh4DG075100 Rh6BG144300 Rh6DG128700
rosa_wichuraiana Rw4G006860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 841
AciI CCGC 1 cut(s) 764
AclWI GGATC 1 cut(s) 494
AcsI RAATTY 2 cut(s) 173, 307
AcuI CTGAAG 1 cut(s) 78
AfaI GTAC 3 cut(s) 152, 563, 695
AfiI CCNNNNNNNGG 6 cut(s) 214, 215, 277, 606, 624, 672
AflIII ACRYGT 1 cut(s) 472
AgsI TTSAA 7 cut(s) 130, 141, 317, 332, 470, 508, 1173
AjnI CCWGG 1 cut(s) 1071
AjuI GAANNNNNNNTTGG 4 cut(s) 698, 730, 1041, 1073
AluBI AGCT 5 cut(s) 41, 56, 836, 932, 1093
AluI AGCT 5 cut(s) 41, 56, 836, 932, 1093
Alw26I GTCTC 3 cut(s) 590, 604, 1154
AlwI GGATC 1 cut(s) 494
AoxI GGCC 2 cut(s) 492, 971
ApeKI GCWGC 4 cut(s) 53, 932, 935, 1126
ApoI RAATTY 2 cut(s) 173, 307
AspS9I GGNCC 1 cut(s) 27
AsuHPI GGTGA 4 cut(s) 504, 926, 1003, 1019
AsuII TTCGAA 1 cut(s) 870
AvaII GGWCC 1 cut(s) 27
BarI GAAGNNNNNNTAC 4 cut(s) 406, 438, 614, 646
BbsI GAAGAC 3 cut(s) 81, 540, 859
BbvI GCAGC 4 cut(s) 65, 919, 922, 1138
BccI CCATC 2 cut(s) 345, 943
BceAI ACGGC 1 cut(s) 650
BciT130I CCWGG 1 cut(s) 1073
BclI TGATCA 1 cut(s) 570
BcoDI GTCTC 3 cut(s) 590, 604, 1154
BfaI CTAG 1 cut(s) 95
BisI GCNGC 5 cut(s) 54, 764, 933, 936, 1127
BlsI GCNGC 5 cut(s) 55, 765, 934, 937, 1128
Bme1390I CCNGG 1 cut(s) 1073
Bme18I GGWCC 1 cut(s) 27
BmgT120I GGNCC 1 cut(s) 27
BmiI GGNNCC 2 cut(s) 28, 781
BmrFI CCNGG 1 cut(s) 1073
BmsI GCATC 2 cut(s) 670, 832
BpiI GAAGAC 3 cut(s) 81, 540, 859
Bpu14I TTCGAA 1 cut(s) 870
BpuEI CTTGAG 1 cut(s) 103
BsaI GGTCTC 3 cut(s) 590, 604, 1154
BsaJI CCNNGG 1 cut(s) 845
Bsc4I CCNNNNNNNGG 6 cut(s) 214, 215, 277, 606, 624, 672
Bse1I ACTGG 3 cut(s) 48, 677, 695
Bse3DI GCAATG 1 cut(s) 660
BseBI CCWGG 1 cut(s) 1073
BseDI CCNNGG 1 cut(s) 845
BseGI GGATG 1 cut(s) 685
BseLI CCNNNNNNNGG 6 cut(s) 214, 215, 277, 606, 624, 672
BseMI GCAATG 1 cut(s) 660
BseMII CTCAG 1 cut(s) 136
BseNI ACTGG 3 cut(s) 48, 677, 695
BseRI GAGGAG 2 cut(s) 27, 1034
BseXI GCAGC 4 cut(s) 65, 919, 922, 1138
BseYI CCCAGC 1 cut(s) 106
BsgI GTGCAG 1 cut(s) 72
BshFI GGCC 2 cut(s) 494, 973
BslI CCNNNNNNNGG 6 cut(s) 214, 215, 277, 606, 624, 672
BsmAI GTCTC 3 cut(s) 590, 604, 1154
BsnI GGCC 2 cut(s) 494, 973
Bso31I GGTCTC 3 cut(s) 590, 604, 1154
Bsp119I TTCGAA 1 cut(s) 870
Bsp143I GATC 2 cut(s) 499, 570
BspACI CCGC 1 cut(s) 764
BspANI GGCC 2 cut(s) 494, 973
BspCNI CTCAG 1 cut(s) 135
BspLI GGNNCC 2 cut(s) 28, 781
BspPI GGATC 1 cut(s) 494
BspT104I TTCGAA 1 cut(s) 870
BspTNI GGTCTC 3 cut(s) 590, 604, 1154
BsrDI GCAATG 1 cut(s) 660
BsrI ACTGG 3 cut(s) 48, 677, 695
BssECI CCNNGG 1 cut(s) 845
BssMI GATC 2 cut(s) 499, 570
BssT1I CCWWGG 1 cut(s) 845
Bst2UI CCWGG 1 cut(s) 1073
Bst4CI ACNGT 3 cut(s) 181, 865, 1165
Bst6I CTCTTC 2 cut(s) 272, 1011
BstBI TTCGAA 1 cut(s) 870
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 2 cut(s) 122, 369
BstF5I GGATG 1 cut(s) 685
BstKTI GATC 2 cut(s) 502, 573
BstMAI GTCTC 3 cut(s) 590, 604, 1154
BstMBI GATC 2 cut(s) 499, 570
BstMWI GCNNNNNNNGC 4 cut(s) 235, 379, 763, 932
BstNI CCWGG 1 cut(s) 1073
BstNSI RCATGY 1 cut(s) 386
BstSCI CCNGG 1 cut(s) 1071
BstV1I GCAGC 4 cut(s) 65, 919, 922, 1138
BstV2I GAAGAC 3 cut(s) 81, 540, 859
BsuRI GGCC 2 cut(s) 494, 973
BtsCI GGATG 1 cut(s) 685
BtsIMutI CAGTG 2 cut(s) 55, 861
Cac8I GCNNGC 1 cut(s) 384
Cfr13I GGNCC 1 cut(s) 27
Csp6I GTAC 3 cut(s) 151, 562, 694
CviAII CATG 5 cut(s) 341, 377, 383, 462, 784
CviQI GTAC 3 cut(s) 151, 562, 694
DdeI CTNAG 2 cut(s) 122, 369
DpnI GATC 2 cut(s) 501, 572
DpnII GATC 2 cut(s) 499, 570
Eam1104I CTCTTC 2 cut(s) 272, 1011
EarI CTCTTC 2 cut(s) 272, 1011
Eco130I CCWWGG 1 cut(s) 845
Eco147I AGGCCT 1 cut(s) 973
Eco31I GGTCTC 3 cut(s) 590, 604, 1154
Eco47I GGWCC 1 cut(s) 27
Eco57I CTGAAG 1 cut(s) 78
EcoRII CCWGG 1 cut(s) 1071
EcoT14I CCWWGG 1 cut(s) 845
EcoT22I ATGCAT 1 cut(s) 467
ErhI CCWWGG 1 cut(s) 845
FaeI CATG 5 cut(s) 344, 380, 386, 465, 787
FatI CATG 5 cut(s) 340, 376, 382, 461, 783
FbaI TGATCA 1 cut(s) 570
FblI GTMKAC 1 cut(s) 841
Fnu4HI GCNGC 5 cut(s) 54, 764, 933, 936, 1127
FokI GGATG 1 cut(s) 692
Fsp4HI GCNGC 5 cut(s) 54, 764, 933, 936, 1127
FspBI CTAG 1 cut(s) 95
GluI GCNGC 5 cut(s) 54, 764, 933, 936, 1127
GsaI CCCAGC 1 cut(s) 110
HaeIII GGCC 2 cut(s) 494, 973
Hin1II CATG 5 cut(s) 344, 380, 386, 465, 787
HincII GTYRAC 1 cut(s) 1038
HindII GTYRAC 1 cut(s) 1038
HinfI GANTC 4 cut(s) 91, 602, 706, 806
HpaI GTTAAC 1 cut(s) 1038
HphI GGTGA 4 cut(s) 504, 926, 1003, 1019
Hpy166II GTNNAC 7 cut(s) 631, 670, 842, 861, 994, 1038, 1115
Hpy188I TCNGA 5 cut(s) 38, 73, 595, 702, 945
Hpy188III TCNNGA 3 cut(s) 82, 389, 1046
Hpy8I GTNNAC 7 cut(s) 631, 670, 842, 861, 994, 1038, 1115
HpyAV CCTTC 4 cut(s) 616, 670, 871, 1043
HpyCH4III ACNGT 3 cut(s) 181, 865, 1165
HpyCH4IV ACGT 2 cut(s) 472, 1117
HpyF10VI GCNNNNNNNGC 4 cut(s) 235, 379, 763, 932
HpyF3I CTNAG 2 cut(s) 122, 369
HpySE526I ACGT 2 cut(s) 472, 1117
Hsp92II CATG 5 cut(s) 344, 380, 386, 465, 787
Ksp22I TGATCA 1 cut(s) 570
KspAI GTTAAC 1 cut(s) 1038
Kzo9I GATC 2 cut(s) 499, 570
LmnI GCTCC 2 cut(s) 391, 833
Lsp1109I GCAGC 4 cut(s) 65, 919, 922, 1138
LweI GCATC 2 cut(s) 670, 832
MaeI CTAG 1 cut(s) 95
MaeII ACGT 2 cut(s) 472, 1117
MaeIII GTNAC 2 cut(s) 181, 952
MalI GATC 2 cut(s) 501, 572
MboI GATC 2 cut(s) 499, 570
MlyI GAGTC 2 cut(s) 611, 815
MmeI TCCRAC 3 cut(s) 54, 271, 811
Mph1103I ATGCAT 1 cut(s) 467
MseI TTAA 5 cut(s) 240, 486, 1001, 1037, 1140
MslI CAYNNNNRTG 1 cut(s) 381
MspA1I CMGCKG 1 cut(s) 56
MspR9I CCNGG 1 cut(s) 1073
MvaI CCWGG 1 cut(s) 1073
MwoI GCNNNNNNNGC 4 cut(s) 235, 379, 763, 932
NdeII GATC 2 cut(s) 499, 570
NlaIII CATG 5 cut(s) 344, 380, 386, 465, 787
NlaIV GGNNCC 2 cut(s) 28, 781
NmeAIII GCCGAG 1 cut(s) 214
NmuCI GTSAC 1 cut(s) 181
NsiI ATGCAT 1 cut(s) 467
NspI RCATGY 1 cut(s) 386
NspV TTCGAA 1 cut(s) 870
PaeI GCATGC 1 cut(s) 386
PceI AGGCCT 1 cut(s) 973
PfeI GAWTC 2 cut(s) 91, 706
PflFI GACNNNGTC 1 cut(s) 601
PfoI TCCNGGA 1 cut(s) 1071
PkrI GCNGC 5 cut(s) 55, 765, 934, 937, 1128
PleI GAGTC 2 cut(s) 610, 814
PpsI GAGTC 2 cut(s) 610, 814
Psp6I CCWGG 1 cut(s) 1071
PspFI CCCAGC 1 cut(s) 106
PspGI CCWGG 1 cut(s) 1071
PspN4I GGNNCC 2 cut(s) 28, 781
PspPI GGNCC 1 cut(s) 27
PsyI GACNNNGTC 1 cut(s) 601
PvuII CAGCTG 1 cut(s) 56
RsaI GTAC 3 cut(s) 152, 563, 695
RsaNI GTAC 3 cut(s) 151, 562, 694
RseI CAYNNNNRTG 1 cut(s) 381
SaqAI TTAA 5 cut(s) 240, 486, 1001, 1037, 1140
SatI GCNGC 5 cut(s) 54, 764, 933, 936, 1127
Sau3AI GATC 2 cut(s) 499, 570
Sau96I GGNCC 1 cut(s) 27
SchI GAGTC 2 cut(s) 611, 815
ScrFI CCNGG 1 cut(s) 1073
SfaNI GCATC 2 cut(s) 670, 832
SfuI TTCGAA 1 cut(s) 870
SinI GGWCC 1 cut(s) 27
SmiMI CAYNNNNRTG 1 cut(s) 381
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
SphI GCATGC 1 cut(s) 386
SseBI AGGCCT 1 cut(s) 973
SsiI CCGC 1 cut(s) 764
SspMI CTAG 1 cut(s) 95
StuI AGGCCT 1 cut(s) 973
StyD4I CCNGG 1 cut(s) 1071
StyI CCWWGG 1 cut(s) 845
TaaI ACNGT 3 cut(s) 181, 865, 1165
TaiI ACGT 2 cut(s) 475, 1120
TaqI TCGA 3 cut(s) 726, 732, 870
TaqII GACCGA 1 cut(s) 614
TauI GCSGC 1 cut(s) 766
TfiI GAWTC 2 cut(s) 91, 706
Tru1I TTAA 5 cut(s) 240, 486, 1001, 1037, 1140
Tru9I TTAA 5 cut(s) 240, 486, 1001, 1037, 1140
TscAI CASTG 2 cut(s) 55, 868
TseFI GTSAC 1 cut(s) 181
TseI GCWGC 4 cut(s) 53, 932, 935, 1126
Tsp45I GTSAC 1 cut(s) 181
TspDTI ATGAA 1 cut(s) 890
TspRI CASTG 2 cut(s) 55, 868
Tth111I GACNNNGTC 1 cut(s) 601
VpaK11BI GGWCC 1 cut(s) 27
XapI RAATTY 2 cut(s) 173, 307
XceI RCATGY 1 cut(s) 386
XmiI GTMKAC 1 cut(s) 841
XspI CTAG 1 cut(s) 95
Zsp2I ATGCAT 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.